MS015015 (gene) Bitter gourd (TR) v1

BLAST of MS015015 vs. NCBI nr
Match: XP_022151810.1 (chloroplast sensor kinase, chloroplastic [Momordica charantia])

HSP 1 Score: 1204.9 bits (3116), Expect = 0.0e+00
Identity = 626/640 (97.81%), Postives = 627/640 (97.97%), Query Frame = 0

Query: 1   MLLSAPALQNPLYYANSSSSFLPFFSNNALKLHSFIRLRPLTFASASSSLTDSSSIRNPS 60
           MLLSAPALQNPLYYANSSSSFLPF SNNALKLHSFIRLRPLTFASASSSLTDSSSIRNPS
Sbjct: 1   MLLSAPALQNPLYYANSSSSFLPFLSNNALKLHSFIRLRPLTFASASSSLTDSSSIRNPS 60

Query: 61  LNPNLLRHVAHTVRDDEEDGPMVSSASAVASAILRASTSPVEFVHRIEKSHNKGLVLPSP 120
           LNPNLLRHVAHTVRDDEEDGPMVSSA AVASAILRASTSPVEFVHRIEKSHNKGLVLPSP
Sbjct: 61  LNPNLLRHVAHTVRDDEEDGPMVSSACAVASAILRASTSPVEFVHRIEKSHNKGLVLPSP 120

Query: 121 DFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVGN 180
           DFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVGN
Sbjct: 121 DFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVGN 180

Query: 181 FSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQSS 240
           FSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQSS
Sbjct: 181 FSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQSS 240

Query: 241 DHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAYV 300
           DHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAYV
Sbjct: 241 DHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAYV 300

Query: 301 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILLQ 360
           MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILLQ
Sbjct: 301 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILLQ 360

Query: 361 GDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKSSNPLSESVKNQLD 420
           GDHMRDTLRQLQDAVSLTK           ANIVHYNEETLKKMYKSSNPLSESVKNQLD
Sbjct: 361 GDHMRDTLRQLQDAVSLTK-----------ANIVHYNEETLKKMYKSSNPLSESVKNQLD 420

Query: 421 NFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIRSCNVSDVLIDLVEAVKP 480
           NFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQR+GIRSCNVSDVLIDLVEAVKP
Sbjct: 421 NFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRYGIRSCNVSDVLIDLVEAVKP 480

Query: 481 LACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGGA 540
           LACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGGA
Sbjct: 481 LACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGGA 540

Query: 541 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR 600
           LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR
Sbjct: 541 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR 600

Query: 601 VISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA 641
           VISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA
Sbjct: 601 VISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA 629

BLAST of MS015015 vs. NCBI nr
Match: KAA0034265.1 (chloroplast sensor kinase [Cucumis melo var. makuwa])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.7e-300
Identity = 543/640 (84.84%), Postives = 579/640 (90.47%), Query Frame = 0

Query: 1   MLLSAPALQNPLYYANSSSSFLPFFSNNALKLHSFIRLRPLTFASASSSLTDSSSIRNPS 60
           +L S+P+LQNPL+ +NSSS F PFFS NALKLHSFIRL+PLTF   SSSLTD+SSI +P+
Sbjct: 2   LLSSSPSLQNPLHCSNSSSPFFPFFSYNALKLHSFIRLKPLTFNCTSSSLTDTSSIPDPT 61

Query: 61  LNPNLLRHVAHTVRDDEEDGPMVSSASAVASAILRASTSPVEFVHRIEKSHNKGLVLPSP 120
               LLRHV+HTVRD  +DG MV SASAVASAIL+ASTSPVEFVHRIE S N GLVLPSP
Sbjct: 62  ----LLRHVSHTVRDTHDDGSMVPSASAVASAILKASTSPVEFVHRIENSQNTGLVLPSP 121

Query: 121 DFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVGN 180
           DF+RLC+EQLDLFRRIVDP+ALLSVYVRPAGSYVMDRLELR VAS+PGVNVTDVV+LVGN
Sbjct: 122 DFQRLCIEQLDLFRRIVDPDALLSVYVRPAGSYVMDRLELRRVASFPGVNVTDVVILVGN 181

Query: 181 FSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQSS 240
           FSVPTGLRAAEA FSSQ+VEVI EH AIVFPMVKHPFVVGFLVAELPNLEME C+ MQS+
Sbjct: 182 FSVPTGLRAAEATFSSQQVEVISEHKAIVFPMVKHPFVVGFLVAELPNLEMETCLDMQSA 241

Query: 241 DHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAYV 300
           D D W YSSP+EA AL+ GS  S  G    TNG LRTY  N DRQ NAFHISRSLAMAYV
Sbjct: 242 DRDPWSYSSPHEAGALVAGSGISAHGFHDATNGSLRTYMFNADRQENAFHISRSLAMAYV 301

Query: 301 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILLQ 360
           MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKK+EIAYEILEDILLQ
Sbjct: 302 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKNEIAYEILEDILLQ 361

Query: 361 GDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKSSNPLSESVKNQLD 420
           GD+MRDTL+QLQDAV LTKALDLS ++   ANIVHYNEETLKKMYKSSNPLSES KNQLD
Sbjct: 362 GDYMRDTLQQLQDAVYLTKALDLSLAI---ANIVHYNEETLKKMYKSSNPLSESAKNQLD 421

Query: 421 NFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIRSCNVSDVLIDLVEAVKP 480
           NFP D S+ RM+GGLVSSN TV+D+EMPMPP +LAPVQR GIRSCNVSDVLIDLVEAVKP
Sbjct: 422 NFPTDASNARMKGGLVSSNNTVRDMEMPMPPTILAPVQRQGIRSCNVSDVLIDLVEAVKP 481

Query: 481 LACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGGA 540
           LA KQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGG+VEI+ST APAGGA
Sbjct: 482 LARKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGKVEIISTAAPAGGA 541

Query: 541 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR 600
           LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILES+GCVVR
Sbjct: 542 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESFGCVVR 601

Query: 601 VISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA 641
           VISPRCSDAALGSGGTRLELWLPS PGTTLHN +IPTQ A
Sbjct: 602 VISPRCSDAALGSGGTRLELWLPSAPGTTLHNLEIPTQGA 634

BLAST of MS015015 vs. NCBI nr
Match: XP_038892932.1 (chloroplast sensor kinase, chloroplastic [Benincasa hispida])

HSP 1 Score: 1040.8 bits (2690), Expect = 4.9e-300
Identity = 548/640 (85.62%), Postives = 577/640 (90.16%), Query Frame = 0

Query: 1   MLLSAPALQNPLYYANSSSSFLPFFSNNALKLHSFIRLRPLTFASASSSLTDSSSIRNPS 60
           MLLS+P+LQNP +Y+NSSS    FFS NALKLHSFIR +PLTF   SSSLTD+SSI NPS
Sbjct: 5   MLLSSPSLQNPHHYSNSSSL---FFSYNALKLHSFIRFKPLTFNCTSSSLTDTSSIPNPS 64

Query: 61  LNPNLLRHVAHTVRDDEEDGPMVSSASAVASAILRASTSPVEFVHRIEKSHNKGLVLPSP 120
           LNP LLRHV HTVRD+++DG MV SASAVASAIL+ASTSPVEFVHR+E S   GLVLPSP
Sbjct: 65  LNPTLLRHVTHTVRDNDDDGSMVPSASAVASAILKASTSPVEFVHRVENSQKTGLVLPSP 124

Query: 121 DFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVGN 180
           DF+RLCVEQLDLFRRIVDP+ALLSVYVRPAGSYVMDRLELR VAS+PGVNVTDVV+LVGN
Sbjct: 125 DFQRLCVEQLDLFRRIVDPDALLSVYVRPAGSYVMDRLELRRVASFPGVNVTDVVILVGN 184

Query: 181 FSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQSS 240
           FSVPTGLRAAEAAFSSQ+VEVI EH AIVFPMVKHPFVVGFLVAELPNLEME+ M MQS 
Sbjct: 185 FSVPTGLRAAEAAFSSQQVEVISEHKAIVFPMVKHPFVVGFLVAELPNLEMEKSMDMQSV 244

Query: 241 DHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAYV 300
           D DLWPYSSP+EA AL+ GS TSTQGIQ+T NG LR YK N DRQ+NAFHISRSLAMAYV
Sbjct: 245 DCDLWPYSSPHEAGALVAGSGTSTQGIQNTANGSLRAYKFNADRQANAFHISRSLAMAYV 304

Query: 301 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILLQ 360
           MDQKAMLLQQSSWQNNLRM NLVDQIRGSLSSIQSLSKMLSVHMKK+EIAYEILEDILLQ
Sbjct: 305 MDQKAMLLQQSSWQNNLRMGNLVDQIRGSLSSIQSLSKMLSVHMKKNEIAYEILEDILLQ 364

Query: 361 GDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKSSNPLSESVKNQLD 420
           G +MRDTL+QLQDAV LTK           ANIVHYNEETLKKMYKSSNPLSESVK QLD
Sbjct: 365 GGYMRDTLQQLQDAVYLTK-----------ANIVHYNEETLKKMYKSSNPLSESVKTQLD 424

Query: 421 NFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIRSCNVSDVLIDLVEAVKP 480
            FP D S+ RMQ GLVSSN  VKD+EMPMPP VLAPVQR GIRSCNVSDVLIDLVEAVKP
Sbjct: 425 YFPTDASNARMQEGLVSSNNMVKDIEMPMPPPVLAPVQRQGIRSCNVSDVLIDLVEAVKP 484

Query: 481 LACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGGA 540
           LA KQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGG+V+I+STTAPAGG 
Sbjct: 485 LARKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGKVDIISTTAPAGGV 544

Query: 541 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR 600
           LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR
Sbjct: 545 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR 604

Query: 601 VISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA 641
           VISPRCSDAALGSGGTRLELWLPS PGTTLHN DIPTQ A
Sbjct: 605 VISPRCSDAALGSGGTRLELWLPSTPGTTLHNLDIPTQGA 630

BLAST of MS015015 vs. NCBI nr
Match: XP_008446148.1 (PREDICTED: chloroplast sensor kinase, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 1028.9 bits (2659), Expect = 1.9e-296
Identity = 538/640 (84.06%), Postives = 572/640 (89.38%), Query Frame = 0

Query: 1   MLLSAPALQNPLYYANSSSSFLPFFSNNALKLHSFIRLRPLTFASASSSLTDSSSIRNPS 60
           +L S+P+LQNPL+ +NSSS F PFFS NALKLHSFIRL+PLTF   SSSLTD+SSI +P+
Sbjct: 2   LLSSSPSLQNPLHCSNSSSPFFPFFSYNALKLHSFIRLKPLTFNCTSSSLTDTSSIPDPT 61

Query: 61  LNPNLLRHVAHTVRDDEEDGPMVSSASAVASAILRASTSPVEFVHRIEKSHNKGLVLPSP 120
               LLRHV+HTVRD  +DG MV SASAVASAIL+ASTSPVEFVHRIE S N GLVLPSP
Sbjct: 62  ----LLRHVSHTVRDTHDDGSMVPSASAVASAILKASTSPVEFVHRIENSQNTGLVLPSP 121

Query: 121 DFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVGN 180
           DF+RLC+EQLDLFRRIVDP+ALLSVYVRPAGSYVMDRLELR VAS+PGVNVTDVV+LVGN
Sbjct: 122 DFQRLCIEQLDLFRRIVDPDALLSVYVRPAGSYVMDRLELRRVASFPGVNVTDVVILVGN 181

Query: 181 FSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQSS 240
           FSVPTGLRAAEA FSSQ+VEVI EH AIVFPMVKHPFVVGFLVAELPNLEME C+ MQS+
Sbjct: 182 FSVPTGLRAAEATFSSQQVEVISEHKAIVFPMVKHPFVVGFLVAELPNLEMETCLDMQSA 241

Query: 241 DHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAYV 300
           D D W YSSP+EA AL+ GS  S  G    TNG LRTY  N DRQ NAFHISRSLAMAYV
Sbjct: 242 DRDPWSYSSPHEAGALVAGSGISAHGFHDATNGSLRTYMFNADRQENAFHISRSLAMAYV 301

Query: 301 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILLQ 360
           MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKK+EIAYEILEDILLQ
Sbjct: 302 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKNEIAYEILEDILLQ 361

Query: 361 GDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKSSNPLSESVKNQLD 420
           GD+MRDTL+QLQDAV LTK           ANIVHYNEETLKKMYKSSNPLSES KNQLD
Sbjct: 362 GDYMRDTLQQLQDAVYLTK-----------ANIVHYNEETLKKMYKSSNPLSESAKNQLD 421

Query: 421 NFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIRSCNVSDVLIDLVEAVKP 480
           NFP D S+ RM+GGLVSSN TV+D+EMPMPP +LAPVQR GIRSCNVSDVLIDLVEAVKP
Sbjct: 422 NFPTDASNARMKGGLVSSNNTVRDMEMPMPPTILAPVQRQGIRSCNVSDVLIDLVEAVKP 481

Query: 481 LACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGGA 540
           LA KQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGG+VEI+ST APAGGA
Sbjct: 482 LARKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGKVEIISTAAPAGGA 541

Query: 541 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR 600
           LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILES+GCVVR
Sbjct: 542 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESFGCVVR 601

Query: 601 VISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA 641
           VISPRCSDAALGSGGTRLELWLPS PGTTLHN +IPTQ A
Sbjct: 602 VISPRCSDAALGSGGTRLELWLPSAPGTTLHNLEIPTQGA 626

BLAST of MS015015 vs. NCBI nr
Match: XP_004135277.1 (chloroplast sensor kinase, chloroplastic isoform X1 [Cucumis sativus] >KGN51745.1 hypothetical protein Csa_009079 [Cucumis sativus])

HSP 1 Score: 1024.2 bits (2647), Expect = 4.7e-295
Identity = 535/641 (83.46%), Postives = 576/641 (89.86%), Query Frame = 0

Query: 1   MLLSAPALQNPLYYANSSSS-FLPFFSNNALKLHSFIRLRPLTFASASSSLTDSSSIRNP 60
           +L S+P+LQNPL+ +NSSSS F PFFS+NALKLHSFIRL+P TF   SSSLTD+SSI +P
Sbjct: 2   LLSSSPSLQNPLHSSNSSSSLFFPFFSHNALKLHSFIRLKPFTFNCTSSSLTDTSSIPDP 61

Query: 61  SLNPNLLRHVAHTVRDDEEDGPMVSSASAVASAILRASTSPVEFVHRIEKSHNKGLVLPS 120
           +    LLRHV+HTVRD  +DG MV SASAVASAIL+ASTSPV+FVHRIE S N GLVLPS
Sbjct: 62  T----LLRHVSHTVRDTHDDGSMVPSASAVASAILKASTSPVDFVHRIENSQNTGLVLPS 121

Query: 121 PDFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVG 180
           PDF+RLC+EQLDLFRRIVDP+ALLSVYVRPAGSYVMDRLELR VAS+PGVNVTDVV+LVG
Sbjct: 122 PDFQRLCIEQLDLFRRIVDPDALLSVYVRPAGSYVMDRLELRRVASFPGVNVTDVVILVG 181

Query: 181 NFSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQS 240
           NFSVPTGLRAAEAAFSSQ+VEVI EH AIVFPMVKHPFVVGFLVAELPNLEME C+ MQS
Sbjct: 182 NFSVPTGLRAAEAAFSSQQVEVISEHKAIVFPMVKHPFVVGFLVAELPNLEMETCLDMQS 241

Query: 241 SDHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAY 300
           +D D W YSSP+EA AL+ GS  ST G  + TNG L+TY  N D Q NAFHISRSLAMAY
Sbjct: 242 ADRDPWSYSSPHEAGALVAGSGISTHGFHNATNGSLKTYMFNADSQENAFHISRSLAMAY 301

Query: 301 VMDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILL 360
           VMDQKAMLLQQSSWQNNLRM+NLVDQIRGSLSSIQSLSKMLSVHMKK+EIAYEILEDILL
Sbjct: 302 VMDQKAMLLQQSSWQNNLRMTNLVDQIRGSLSSIQSLSKMLSVHMKKNEIAYEILEDILL 361

Query: 361 QGDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKSSNPLSESVKNQL 420
           QGD+MR+TL+QLQDAV LTK           ANIVHYNEETLKKMYKSSNPLSESVKNQL
Sbjct: 362 QGDYMRNTLQQLQDAVYLTK-----------ANIVHYNEETLKKMYKSSNPLSESVKNQL 421

Query: 421 DNFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIRSCNVSDVLIDLVEAVK 480
           DNFP D S+ RM+GGLVSSN TV+D+EMPMPP +LAP+QR GIRSCNVSDVLIDLVEAVK
Sbjct: 422 DNFPTDASNPRMKGGLVSSNNTVRDMEMPMPPTILAPIQRQGIRSCNVSDVLIDLVEAVK 481

Query: 481 PLACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGG 540
           PLA KQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGG+VEI+ST APAGG
Sbjct: 482 PLARKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGKVEIISTAAPAGG 541

Query: 541 ALIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVV 600
           ALIV+DDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVV
Sbjct: 542 ALIVVDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVV 601

Query: 601 RVISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA 641
           RVISPRCSDAALGSGGTRLELWLPS PGTTLHN +IPTQ A
Sbjct: 602 RVISPRCSDAALGSGGTRLELWLPSTPGTTLHNLEIPTQSA 627

BLAST of MS015015 vs. ExPASy Swiss-Prot
Match: F4HVG8 (Chloroplast sensor kinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CSK PE=1 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 1.2e-184
Identity = 375/640 (58.59%), Postives = 467/640 (72.97%), Query Frame = 0

Query: 1   MLLSAPALQ-----NP-LYYANSSSSFLPFFSNNALKLHSFIRLRPLTFASASSSLTDSS 60
           MLLSA A Q     NP L+++NS  +  P  SN +LKL +         AS+SSS + SS
Sbjct: 1   MLLSAIASQTLLSSNPNLHFSNSIPNPRP--SNPSLKLLN---------ASSSSSSSSSS 60

Query: 61  SIRNPSLNPNLLRHVAHTVRDDEED----GPMVSSASAVASAILRASTSPVEFVHRIEKS 120
           SI         LR+V HTV ++E +      MV+SASA+ASAI  AST+PVEF   IEK 
Sbjct: 61  SIFTRG-----LRYVNHTVSNEESEPGGGETMVASASAIASAIRGASTTPVEFTQMIEKD 120

Query: 121 HNK-GLVLPSPDFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGV 180
           H K  ++LPSPDF+RLC+EQLDLFR+IVDP A+LS+YVRPAGSYVMDRLELR V  YP V
Sbjct: 121 HLKTKIILPSPDFQRLCLEQLDLFRQIVDPNAVLSIYVRPAGSYVMDRLELRRVTCYPSV 180

Query: 181 NVTDVVLLVGNFSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELP-N 240
           N  DVV+LVGNF +P GLRAAEA+ SSQ+VE++ +H A VFPMVKHPFVVGFLVAELP  
Sbjct: 181 NAGDVVILVGNFGIPAGLRAAEASLSSQQVELVSKHRAAVFPMVKHPFVVGFLVAELPVE 240

Query: 241 LEMERCMGMQSSDHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKL-NQDRQSN 300
            E E     +   H +  + SP EA AL   + T +  ++      L + K+  ++++S 
Sbjct: 241 AEEEEEEEEEEKPHGVNQFLSPEEAYALPASANTKSPRVK------LPSVKVFTEEQRSY 300

Query: 301 AFHISRSLAMAYVMDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKS 360
           A +ISR+LAMAYVMDQK MLLQQSSWQNN+RMS LV+QIRG LS++++LSKMLS H K++
Sbjct: 301 AINISRTLAMAYVMDQKTMLLQQSSWQNNVRMSKLVEQIRGPLSTMRTLSKMLSTHTKRN 360

Query: 361 EIAYEILEDILLQGDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKS 420
           +I+++I+ED+++QGD ++DTL +LQDAV LTK           ANIV +NEE LKK+ K+
Sbjct: 361 QISHDIVEDLIVQGDQIKDTLEELQDAVHLTK-----------ANIVRHNEEALKKINKT 420

Query: 421 SNPLSESVKNQLDNFP-KDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIRSCN 480
            N    S     D      +SS R+     S  + + D EMPMPP  LAP+Q H IR C+
Sbjct: 421 HNETRRSKYEHKDPIDGSQISSTRL-----SLGSGLDDSEMPMPPLALAPLQMHSIRPCD 480

Query: 481 VSDVLIDLVEAVKPLACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGG 540
           +S+VL+D+VE V+PLA  QQR+VEL E + S+Q+AVEE +LRQALSNLIEGALLRT VGG
Sbjct: 481 ISNVLLDMVETVRPLALTQQRVVELGENSASLQVAVEEPALRQALSNLIEGALLRTHVGG 540

Query: 541 RVEIVSTTAPAGGALIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLT 600
           +VEI+ST APAGG+L+VIDDDGPDM YMTQMHSLTPFGA+LLS+  VEDNMTWNFVAGLT
Sbjct: 541 KVEILSTRAPAGGSLVVIDDDGPDMRYMTQMHSLTPFGAELLSENMVEDNMTWNFVAGLT 600

Query: 601 VACEILESYGCVVRVISPRCSDAALGSGGTRLELWLPSIP 627
           VA EILESYGCV+RVISPR SDAALG+GGTR+ELWLP+ P
Sbjct: 601 VAREILESYGCVIRVISPRSSDAALGAGGTRVELWLPAFP 602

BLAST of MS015015 vs. ExPASy TrEMBL
Match: A0A6J1DC84 (chloroplast sensor kinase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019704 PE=4 SV=1)

HSP 1 Score: 1204.9 bits (3116), Expect = 0.0e+00
Identity = 626/640 (97.81%), Postives = 627/640 (97.97%), Query Frame = 0

Query: 1   MLLSAPALQNPLYYANSSSSFLPFFSNNALKLHSFIRLRPLTFASASSSLTDSSSIRNPS 60
           MLLSAPALQNPLYYANSSSSFLPF SNNALKLHSFIRLRPLTFASASSSLTDSSSIRNPS
Sbjct: 1   MLLSAPALQNPLYYANSSSSFLPFLSNNALKLHSFIRLRPLTFASASSSLTDSSSIRNPS 60

Query: 61  LNPNLLRHVAHTVRDDEEDGPMVSSASAVASAILRASTSPVEFVHRIEKSHNKGLVLPSP 120
           LNPNLLRHVAHTVRDDEEDGPMVSSA AVASAILRASTSPVEFVHRIEKSHNKGLVLPSP
Sbjct: 61  LNPNLLRHVAHTVRDDEEDGPMVSSACAVASAILRASTSPVEFVHRIEKSHNKGLVLPSP 120

Query: 121 DFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVGN 180
           DFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVGN
Sbjct: 121 DFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVGN 180

Query: 181 FSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQSS 240
           FSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQSS
Sbjct: 181 FSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQSS 240

Query: 241 DHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAYV 300
           DHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAYV
Sbjct: 241 DHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAYV 300

Query: 301 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILLQ 360
           MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILLQ
Sbjct: 301 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILLQ 360

Query: 361 GDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKSSNPLSESVKNQLD 420
           GDHMRDTLRQLQDAVSLTK           ANIVHYNEETLKKMYKSSNPLSESVKNQLD
Sbjct: 361 GDHMRDTLRQLQDAVSLTK-----------ANIVHYNEETLKKMYKSSNPLSESVKNQLD 420

Query: 421 NFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIRSCNVSDVLIDLVEAVKP 480
           NFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQR+GIRSCNVSDVLIDLVEAVKP
Sbjct: 421 NFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRYGIRSCNVSDVLIDLVEAVKP 480

Query: 481 LACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGGA 540
           LACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGGA
Sbjct: 481 LACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGGA 540

Query: 541 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR 600
           LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR
Sbjct: 541 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR 600

Query: 601 VISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA 641
           VISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA
Sbjct: 601 VISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA 629

BLAST of MS015015 vs. ExPASy TrEMBL
Match: A0A5A7SYI9 (Chloroplast sensor kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G002640 PE=4 SV=1)

HSP 1 Score: 1041.2 bits (2691), Expect = 1.8e-300
Identity = 543/640 (84.84%), Postives = 579/640 (90.47%), Query Frame = 0

Query: 1   MLLSAPALQNPLYYANSSSSFLPFFSNNALKLHSFIRLRPLTFASASSSLTDSSSIRNPS 60
           +L S+P+LQNPL+ +NSSS F PFFS NALKLHSFIRL+PLTF   SSSLTD+SSI +P+
Sbjct: 2   LLSSSPSLQNPLHCSNSSSPFFPFFSYNALKLHSFIRLKPLTFNCTSSSLTDTSSIPDPT 61

Query: 61  LNPNLLRHVAHTVRDDEEDGPMVSSASAVASAILRASTSPVEFVHRIEKSHNKGLVLPSP 120
               LLRHV+HTVRD  +DG MV SASAVASAIL+ASTSPVEFVHRIE S N GLVLPSP
Sbjct: 62  ----LLRHVSHTVRDTHDDGSMVPSASAVASAILKASTSPVEFVHRIENSQNTGLVLPSP 121

Query: 121 DFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVGN 180
           DF+RLC+EQLDLFRRIVDP+ALLSVYVRPAGSYVMDRLELR VAS+PGVNVTDVV+LVGN
Sbjct: 122 DFQRLCIEQLDLFRRIVDPDALLSVYVRPAGSYVMDRLELRRVASFPGVNVTDVVILVGN 181

Query: 181 FSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQSS 240
           FSVPTGLRAAEA FSSQ+VEVI EH AIVFPMVKHPFVVGFLVAELPNLEME C+ MQS+
Sbjct: 182 FSVPTGLRAAEATFSSQQVEVISEHKAIVFPMVKHPFVVGFLVAELPNLEMETCLDMQSA 241

Query: 241 DHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAYV 300
           D D W YSSP+EA AL+ GS  S  G    TNG LRTY  N DRQ NAFHISRSLAMAYV
Sbjct: 242 DRDPWSYSSPHEAGALVAGSGISAHGFHDATNGSLRTYMFNADRQENAFHISRSLAMAYV 301

Query: 301 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILLQ 360
           MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKK+EIAYEILEDILLQ
Sbjct: 302 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKNEIAYEILEDILLQ 361

Query: 361 GDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKSSNPLSESVKNQLD 420
           GD+MRDTL+QLQDAV LTKALDLS ++   ANIVHYNEETLKKMYKSSNPLSES KNQLD
Sbjct: 362 GDYMRDTLQQLQDAVYLTKALDLSLAI---ANIVHYNEETLKKMYKSSNPLSESAKNQLD 421

Query: 421 NFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIRSCNVSDVLIDLVEAVKP 480
           NFP D S+ RM+GGLVSSN TV+D+EMPMPP +LAPVQR GIRSCNVSDVLIDLVEAVKP
Sbjct: 422 NFPTDASNARMKGGLVSSNNTVRDMEMPMPPTILAPVQRQGIRSCNVSDVLIDLVEAVKP 481

Query: 481 LACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGGA 540
           LA KQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGG+VEI+ST APAGGA
Sbjct: 482 LARKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGKVEIISTAAPAGGA 541

Query: 541 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR 600
           LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILES+GCVVR
Sbjct: 542 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESFGCVVR 601

Query: 601 VISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA 641
           VISPRCSDAALGSGGTRLELWLPS PGTTLHN +IPTQ A
Sbjct: 602 VISPRCSDAALGSGGTRLELWLPSAPGTTLHNLEIPTQGA 634

BLAST of MS015015 vs. ExPASy TrEMBL
Match: A0A1S3BF75 (chloroplast sensor kinase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488961 PE=4 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 9.3e-297
Identity = 538/640 (84.06%), Postives = 572/640 (89.38%), Query Frame = 0

Query: 1   MLLSAPALQNPLYYANSSSSFLPFFSNNALKLHSFIRLRPLTFASASSSLTDSSSIRNPS 60
           +L S+P+LQNPL+ +NSSS F PFFS NALKLHSFIRL+PLTF   SSSLTD+SSI +P+
Sbjct: 2   LLSSSPSLQNPLHCSNSSSPFFPFFSYNALKLHSFIRLKPLTFNCTSSSLTDTSSIPDPT 61

Query: 61  LNPNLLRHVAHTVRDDEEDGPMVSSASAVASAILRASTSPVEFVHRIEKSHNKGLVLPSP 120
               LLRHV+HTVRD  +DG MV SASAVASAIL+ASTSPVEFVHRIE S N GLVLPSP
Sbjct: 62  ----LLRHVSHTVRDTHDDGSMVPSASAVASAILKASTSPVEFVHRIENSQNTGLVLPSP 121

Query: 121 DFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVGN 180
           DF+RLC+EQLDLFRRIVDP+ALLSVYVRPAGSYVMDRLELR VAS+PGVNVTDVV+LVGN
Sbjct: 122 DFQRLCIEQLDLFRRIVDPDALLSVYVRPAGSYVMDRLELRRVASFPGVNVTDVVILVGN 181

Query: 181 FSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQSS 240
           FSVPTGLRAAEA FSSQ+VEVI EH AIVFPMVKHPFVVGFLVAELPNLEME C+ MQS+
Sbjct: 182 FSVPTGLRAAEATFSSQQVEVISEHKAIVFPMVKHPFVVGFLVAELPNLEMETCLDMQSA 241

Query: 241 DHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAYV 300
           D D W YSSP+EA AL+ GS  S  G    TNG LRTY  N DRQ NAFHISRSLAMAYV
Sbjct: 242 DRDPWSYSSPHEAGALVAGSGISAHGFHDATNGSLRTYMFNADRQENAFHISRSLAMAYV 301

Query: 301 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILLQ 360
           MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKK+EIAYEILEDILLQ
Sbjct: 302 MDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKNEIAYEILEDILLQ 361

Query: 361 GDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKSSNPLSESVKNQLD 420
           GD+MRDTL+QLQDAV LTK           ANIVHYNEETLKKMYKSSNPLSES KNQLD
Sbjct: 362 GDYMRDTLQQLQDAVYLTK-----------ANIVHYNEETLKKMYKSSNPLSESAKNQLD 421

Query: 421 NFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIRSCNVSDVLIDLVEAVKP 480
           NFP D S+ RM+GGLVSSN TV+D+EMPMPP +LAPVQR GIRSCNVSDVLIDLVEAVKP
Sbjct: 422 NFPTDASNARMKGGLVSSNNTVRDMEMPMPPTILAPVQRQGIRSCNVSDVLIDLVEAVKP 481

Query: 481 LACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGGA 540
           LA KQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGG+VEI+ST APAGGA
Sbjct: 482 LARKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGKVEIISTAAPAGGA 541

Query: 541 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVVR 600
           LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILES+GCVVR
Sbjct: 542 LIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESFGCVVR 601

Query: 601 VISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA 641
           VISPRCSDAALGSGGTRLELWLPS PGTTLHN +IPTQ A
Sbjct: 602 VISPRCSDAALGSGGTRLELWLPSAPGTTLHNLEIPTQGA 626

BLAST of MS015015 vs. ExPASy TrEMBL
Match: A0A0A0KQ24 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G598040 PE=4 SV=1)

HSP 1 Score: 1024.2 bits (2647), Expect = 2.3e-295
Identity = 535/641 (83.46%), Postives = 576/641 (89.86%), Query Frame = 0

Query: 1   MLLSAPALQNPLYYANSSSS-FLPFFSNNALKLHSFIRLRPLTFASASSSLTDSSSIRNP 60
           +L S+P+LQNPL+ +NSSSS F PFFS+NALKLHSFIRL+P TF   SSSLTD+SSI +P
Sbjct: 2   LLSSSPSLQNPLHSSNSSSSLFFPFFSHNALKLHSFIRLKPFTFNCTSSSLTDTSSIPDP 61

Query: 61  SLNPNLLRHVAHTVRDDEEDGPMVSSASAVASAILRASTSPVEFVHRIEKSHNKGLVLPS 120
           +    LLRHV+HTVRD  +DG MV SASAVASAIL+ASTSPV+FVHRIE S N GLVLPS
Sbjct: 62  T----LLRHVSHTVRDTHDDGSMVPSASAVASAILKASTSPVDFVHRIENSQNTGLVLPS 121

Query: 121 PDFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVVLLVG 180
           PDF+RLC+EQLDLFRRIVDP+ALLSVYVRPAGSYVMDRLELR VAS+PGVNVTDVV+LVG
Sbjct: 122 PDFQRLCIEQLDLFRRIVDPDALLSVYVRPAGSYVMDRLELRRVASFPGVNVTDVVILVG 181

Query: 181 NFSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCMGMQS 240
           NFSVPTGLRAAEAAFSSQ+VEVI EH AIVFPMVKHPFVVGFLVAELPNLEME C+ MQS
Sbjct: 182 NFSVPTGLRAAEAAFSSQQVEVISEHKAIVFPMVKHPFVVGFLVAELPNLEMETCLDMQS 241

Query: 241 SDHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSLAMAY 300
           +D D W YSSP+EA AL+ GS  ST G  + TNG L+TY  N D Q NAFHISRSLAMAY
Sbjct: 242 ADRDPWSYSSPHEAGALVAGSGISTHGFHNATNGSLKTYMFNADSQENAFHISRSLAMAY 301

Query: 301 VMDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILEDILL 360
           VMDQKAMLLQQSSWQNNLRM+NLVDQIRGSLSSIQSLSKMLSVHMKK+EIAYEILEDILL
Sbjct: 302 VMDQKAMLLQQSSWQNNLRMTNLVDQIRGSLSSIQSLSKMLSVHMKKNEIAYEILEDILL 361

Query: 361 QGDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKSSNPLSESVKNQL 420
           QGD+MR+TL+QLQDAV LTK           ANIVHYNEETLKKMYKSSNPLSESVKNQL
Sbjct: 362 QGDYMRNTLQQLQDAVYLTK-----------ANIVHYNEETLKKMYKSSNPLSESVKNQL 421

Query: 421 DNFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIRSCNVSDVLIDLVEAVK 480
           DNFP D S+ RM+GGLVSSN TV+D+EMPMPP +LAP+QR GIRSCNVSDVLIDLVEAVK
Sbjct: 422 DNFPTDASNPRMKGGLVSSNNTVRDMEMPMPPTILAPIQRQGIRSCNVSDVLIDLVEAVK 481

Query: 481 PLACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTAPAGG 540
           PLA KQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGG+VEI+ST APAGG
Sbjct: 482 PLARKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGKVEIISTAAPAGG 541

Query: 541 ALIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVV 600
           ALIV+DDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVV
Sbjct: 542 ALIVVDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESYGCVV 601

Query: 601 RVISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA 641
           RVISPRCSDAALGSGGTRLELWLPS PGTTLHN +IPTQ A
Sbjct: 602 RVISPRCSDAALGSGGTRLELWLPSTPGTTLHNLEIPTQSA 627

BLAST of MS015015 vs. ExPASy TrEMBL
Match: A0A6J1JJC3 (chloroplast sensor kinase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111485623 PE=4 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 6.4e-290
Identity = 541/645 (83.88%), Postives = 569/645 (88.22%), Query Frame = 0

Query: 1   MLLSAPALQNPLYYANSSSSFLPFFSNNALKLH-SFIRLRPLTFASASSSLTDSSSIRNP 60
           MLLS P L+NPL+Y+NSSS  LPFFS NALKLH SFIRL+PLTF SASSS  ++SSI NP
Sbjct: 1   MLLSWPPLKNPLFYSNSSSPILPFFSYNALKLHNSFIRLKPLTFTSASSSF-NTSSISNP 60

Query: 61  SLNPNLLRHVAHTV----RDDEEDGPMVSSASAVASAILRASTSPVEFVHRIEKSHNKGL 120
           S    LLRHV HTV     DD++DG MV SASAVAS ILRASTSPVEFVHRIE     GL
Sbjct: 61  S----LLRHVTHTVCDDDDDDDDDGSMVPSASAVASVILRASTSPVEFVHRIENRQTSGL 120

Query: 121 VLPSPDFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGVNVTDVV 180
           VLPSPDF+RLCVEQLDLFRRIVDPEALLSVYVRPAGS+VMDRLELR V S+PGVN TDVV
Sbjct: 121 VLPSPDFQRLCVEQLDLFRRIVDPEALLSVYVRPAGSFVMDRLELRRVVSFPGVNATDVV 180

Query: 181 LLVGNFSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELPNLEMERCM 240
           +LVGNFSVPTGLRAAEA FSSQ+VEVIPEH AIVFPMVKHPFVVGFLVAELPNLEM+   
Sbjct: 181 ILVGNFSVPTGLRAAEATFSSQQVEVIPEHKAIVFPMVKHPFVVGFLVAELPNLEMD--- 240

Query: 241 GMQSSDHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKLNQDRQSNAFHISRSL 300
            MQS+D D WPYSSP+EA ALL G  T+TQGIQSTTNG LRTYKLN DRQ+NAFHISRSL
Sbjct: 241 -MQSADCDPWPYSSPHEAGALLAGFGTNTQGIQSTTNGSLRTYKLNADRQANAFHISRSL 300

Query: 301 AMAYVMDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKSEIAYEILE 360
           AMAYVMDQKAMLLQQSSWQNNLRM+NLVDQIR SLSSIQ+LSKMLSVHMKKSEIAYEILE
Sbjct: 301 AMAYVMDQKAMLLQQSSWQNNLRMTNLVDQIRNSLSSIQTLSKMLSVHMKKSEIAYEILE 360

Query: 361 DILLQGDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKSSNPLSESV 420
           DIL+QG+HMRDTL+QLQDAV LTK           ANIVHYNEETLKKMY SSNPLSESV
Sbjct: 361 DILVQGNHMRDTLQQLQDAVYLTK-----------ANIVHYNEETLKKMYMSSNPLSESV 420

Query: 421 KNQLDNFPKDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIRSCNVSDVLIDLV 480
           KNQLDN+P D SS R QGGLVSS   ++DLEMPMPP VLAPVQR GIRSCNVSDVL DLV
Sbjct: 421 KNQLDNYPTDASSSRTQGGLVSSKNMIEDLEMPMPPTVLAPVQRQGIRSCNVSDVLNDLV 480

Query: 481 EAVKPLACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGGRVEIVSTTA 540
           EAVKPLA KQQRIVELSEQ CSMQIAVEESSLRQALSNLIEGALLRTRVGG+VEIVSTTA
Sbjct: 481 EAVKPLASKQQRIVELSEQVCSMQIAVEESSLRQALSNLIEGALLRTRVGGKVEIVSTTA 540

Query: 541 PAGGALIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLTVACEILESY 600
           PAGGALIVIDDDGPDMHYMTQMHSLT FGADLLSKERVEDNMTWNFVAGLTVACEILESY
Sbjct: 541 PAGGALIVIDDDGPDMHYMTQMHSLTAFGADLLSKERVEDNMTWNFVAGLTVACEILESY 600

Query: 601 GCVVRVISPRCSDAALGSGGTRLELWLPSIPGTTLHNFDIPTQEA 641
           GCVVRVISPRCSDAALGSGGTRLELWLPS PGT+LHN DIPT  A
Sbjct: 601 GCVVRVISPRCSDAALGSGGTRLELWLPSTPGTSLHNLDIPTSGA 625

BLAST of MS015015 vs. TAIR 10
Match: AT1G67840.1 (chloroplast sensor kinase )

HSP 1 Score: 647.9 bits (1670), Expect = 8.6e-186
Identity = 375/640 (58.59%), Postives = 467/640 (72.97%), Query Frame = 0

Query: 1   MLLSAPALQ-----NP-LYYANSSSSFLPFFSNNALKLHSFIRLRPLTFASASSSLTDSS 60
           MLLSA A Q     NP L+++NS  +  P  SN +LKL +         AS+SSS + SS
Sbjct: 1   MLLSAIASQTLLSSNPNLHFSNSIPNPRP--SNPSLKLLN---------ASSSSSSSSSS 60

Query: 61  SIRNPSLNPNLLRHVAHTVRDDEED----GPMVSSASAVASAILRASTSPVEFVHRIEKS 120
           SI         LR+V HTV ++E +      MV+SASA+ASAI  AST+PVEF   IEK 
Sbjct: 61  SIFTRG-----LRYVNHTVSNEESEPGGGETMVASASAIASAIRGASTTPVEFTQMIEKD 120

Query: 121 HNK-GLVLPSPDFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGV 180
           H K  ++LPSPDF+RLC+EQLDLFR+IVDP A+LS+YVRPAGSYVMDRLELR V  YP V
Sbjct: 121 HLKTKIILPSPDFQRLCLEQLDLFRQIVDPNAVLSIYVRPAGSYVMDRLELRRVTCYPSV 180

Query: 181 NVTDVVLLVGNFSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELP-N 240
           N  DVV+LVGNF +P GLRAAEA+ SSQ+VE++ +H A VFPMVKHPFVVGFLVAELP  
Sbjct: 181 NAGDVVILVGNFGIPAGLRAAEASLSSQQVELVSKHRAAVFPMVKHPFVVGFLVAELPVE 240

Query: 241 LEMERCMGMQSSDHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKL-NQDRQSN 300
            E E     +   H +  + SP EA AL   + T +  ++      L + K+  ++++S 
Sbjct: 241 AEEEEEEEEEEKPHGVNQFLSPEEAYALPASANTKSPRVK------LPSVKVFTEEQRSY 300

Query: 301 AFHISRSLAMAYVMDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKS 360
           A +ISR+LAMAYVMDQK MLLQQSSWQNN+RMS LV+QIRG LS++++LSKMLS H K++
Sbjct: 301 AINISRTLAMAYVMDQKTMLLQQSSWQNNVRMSKLVEQIRGPLSTMRTLSKMLSTHTKRN 360

Query: 361 EIAYEILEDILLQGDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKS 420
           +I+++I+ED+++QGD ++DTL +LQDAV LTK           ANIV +NEE LKK+ K+
Sbjct: 361 QISHDIVEDLIVQGDQIKDTLEELQDAVHLTK-----------ANIVRHNEEALKKINKT 420

Query: 421 SNPLSESVKNQLDNFP-KDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIRSCN 480
            N    S     D      +SS R+     S  + + D EMPMPP  LAP+Q H IR C+
Sbjct: 421 HNETRRSKYEHKDPIDGSQISSTRL-----SLGSGLDDSEMPMPPLALAPLQMHSIRPCD 480

Query: 481 VSDVLIDLVEAVKPLACKQQRIVELSEQACSMQIAVEESSLRQALSNLIEGALLRTRVGG 540
           +S+VL+D+VE V+PLA  QQR+VEL E + S+Q+AVEE +LRQALSNLIEGALLRT VGG
Sbjct: 481 ISNVLLDMVETVRPLALTQQRVVELGENSASLQVAVEEPALRQALSNLIEGALLRTHVGG 540

Query: 541 RVEIVSTTAPAGGALIVIDDDGPDMHYMTQMHSLTPFGADLLSKERVEDNMTWNFVAGLT 600
           +VEI+ST APAGG+L+VIDDDGPDM YMTQMHSLTPFGA+LLS+  VEDNMTWNFVAGLT
Sbjct: 541 KVEILSTRAPAGGSLVVIDDDGPDMRYMTQMHSLTPFGAELLSENMVEDNMTWNFVAGLT 600

Query: 601 VACEILESYGCVVRVISPRCSDAALGSGGTRLELWLPSIP 627
           VA EILESYGCV+RVISPR SDAALG+GGTR+ELWLP+ P
Sbjct: 601 VAREILESYGCVIRVISPRSSDAALGAGGTRVELWLPAFP 602

BLAST of MS015015 vs. TAIR 10
Match: AT1G67840.2 (chloroplast sensor kinase )

HSP 1 Score: 392.1 bits (1006), Expect = 8.5e-109
Identity = 249/477 (52.20%), Postives = 320/477 (67.09%), Query Frame = 0

Query: 1   MLLSAPALQ-----NP-LYYANSSSSFLPFFSNNALKLHSFIRLRPLTFASASSSLTDSS 60
           MLLSA A Q     NP L+++NS  +  P  SN +LKL +         AS+SSS + SS
Sbjct: 1   MLLSAIASQTLLSSNPNLHFSNSIPNPRP--SNPSLKLLN---------ASSSSSSSSSS 60

Query: 61  SIRNPSLNPNLLRHVAHTVRDDEED----GPMVSSASAVASAILRASTSPVEFVHRIEKS 120
           SI         LR+V HTV ++E +      MV+SASA+ASAI  AST+PVEF   IEK 
Sbjct: 61  SIFTRG-----LRYVNHTVSNEESEPGGGETMVASASAIASAIRGASTTPVEFTQMIEKD 120

Query: 121 HNK-GLVLPSPDFRRLCVEQLDLFRRIVDPEALLSVYVRPAGSYVMDRLELRCVASYPGV 180
           H K  ++LPSPDF+RLC+EQLDLFR+IVDP A+LS+YVRPAGSYVMDRLELR V  YP V
Sbjct: 121 HLKTKIILPSPDFQRLCLEQLDLFRQIVDPNAVLSIYVRPAGSYVMDRLELRRVTCYPSV 180

Query: 181 NVTDVVLLVGNFSVPTGLRAAEAAFSSQRVEVIPEHNAIVFPMVKHPFVVGFLVAELP-N 240
           N  DVV+LVGNF +P GLRAAEA+ SSQ+VE++ +H A VFPMVKHPFVVGFLVAELP  
Sbjct: 181 NAGDVVILVGNFGIPAGLRAAEASLSSQQVELVSKHRAAVFPMVKHPFVVGFLVAELPVE 240

Query: 241 LEMERCMGMQSSDHDLWPYSSPYEACALLTGSETSTQGIQSTTNGPLRTYKL-NQDRQSN 300
            E E     +   H +  + SP EA AL   + T +  ++      L + K+  ++++S 
Sbjct: 241 AEEEEEEEEEEKPHGVNQFLSPEEAYALPASANTKSPRVK------LPSVKVFTEEQRSY 300

Query: 301 AFHISRSLAMAYVMDQKAMLLQQSSWQNNLRMSNLVDQIRGSLSSIQSLSKMLSVHMKKS 360
           A +ISR+LAMAYVMDQK MLLQQSSWQNN+RMS LV+QIRG LS++++LSKMLS H K++
Sbjct: 301 AINISRTLAMAYVMDQKTMLLQQSSWQNNVRMSKLVEQIRGPLSTMRTLSKMLSTHTKRN 360

Query: 361 EIAYEILEDILLQGDHMRDTLRQLQDAVSLTKALDLSFSLAITANIVHYNEETLKKMYKS 420
           +I+++I+ED+++QGD ++DTL +LQDAV LTK           ANIV +NEE LKK+ K+
Sbjct: 361 QISHDIVEDLIVQGDQIKDTLEELQDAVHLTK-----------ANIVRHNEEALKKINKT 420

Query: 421 SNPLSESVKNQLDNFP-KDVSSLRMQGGLVSSNTTVKDLEMPMPPAVLAPVQRHGIR 464
            N    S     D      +SS R+     S  + + D EMPMPP  LAP+Q H IR
Sbjct: 421 HNETRRSKYEHKDPIDGSQISSTRL-----SLGSGLDDSEMPMPPLALAPLQMHSIR 439