MS014266 (gene) Bitter gourd (TR) v1

Overview
NameMS014266
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncyclin-dependent protein kinase inhibitor SMR14-like
Locationscaffold5: 1773147 .. 1773458 (+)
RNA-Seq ExpressionMS014266
SyntenyMS014266
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGGAAGAAGATCGGTTGAAAGACAAGTTTGATATTTTACTGTCCACTTTGGGAGTACGGTTGCCGCCGTCCGTAGGCGAGGAAAATGAAGATCGGCCCGAGGATTTGAAGACTCCCACATCCTCGGAGGCTGCGGCGGTTCTTCAGTGTCCGCCGCCGCCGAGAAAGCCGAAAAGGCTGCCGTCGATGAAACGGAAGGCGCGTGGGCGGTCAATTGTGATGCCCCATAATTTGTTCGTCGAAATGGAGTTGATGTTTCCTCCGCATGATCTCCTCGGCGGTAAGGTTGTTAAGAAAGTTAAACGACCGGCG

mRNA sequence

GAGGAAGAAGATCGGTTGAAAGACAAGTTTGATATTTTACTGTCCACTTTGGGAGTACGGTTGCCGCCGTCCGTAGGCGAGGAAAATGAAGATCGGCCCGAGGATTTGAAGACTCCCACATCCTCGGAGGCTGCGGCGGTTCTTCAGTGTCCGCCGCCGCCGAGAAAGCCGAAAAGGCTGCCGTCGATGAAACGGAAGGCGCGTGGGCGGTCAATTGTGATGCCCCATAATTTGTTCGTCGAAATGGAGTTGATGTTTCCTCCGCATGATCTCCTCGGCGGTAAGGTTGTTAAGAAAGTTAAACGACCGGCG

Coding sequence (CDS)

GAGGAAGAAGATCGGTTGAAAGACAAGTTTGATATTTTACTGTCCACTTTGGGAGTACGGTTGCCGCCGTCCGTAGGCGAGGAAAATGAAGATCGGCCCGAGGATTTGAAGACTCCCACATCCTCGGAGGCTGCGGCGGTTCTTCAGTGTCCGCCGCCGCCGAGAAAGCCGAAAAGGCTGCCGTCGATGAAACGGAAGGCGCGTGGGCGGTCAATTGTGATGCCCCATAATTTGTTCGTCGAAATGGAGTTGATGTTTCCTCCGCATGATCTCCTCGGCGGTAAGGTTGTTAAGAAAGTTAAACGACCGGCG

Protein sequence

EEEDRLKDKFDILLSTLGVRLPPSVGEENEDRPEDLKTPTSSEAAAVLQCPPPPRKPKRLPSMKRKARGRSIVMPHNLFVEMELMFPPHDLLGGKVVKKVKRPA
Homology
BLAST of MS014266 vs. NCBI nr
Match: XP_023006801.1 (cyclin-dependent protein kinase inhibitor SMR14-like [Cucurbita maxima])

HSP 1 Score: 104.8 bits (260), Expect = 4.7e-19
Identity = 63/114 (55.26%), Postives = 78/114 (68.42%), Query Frame = 0

Query: 1   EEEDRLKDKFDILLSTLGVRLPPSV-----GEENED-RPEDLKTPTSSEAAAVLQCPPPP 60
           E   + +DKF+ILLSTL ++L  S       EE ED R + +KTP S E A VLQCPPPP
Sbjct: 2   EHHPQKEDKFEILLSTLELKLQSSCFRHEDEEEEEDCRDDFVKTPRSLEPAVVLQCPPPP 61

Query: 61  RKPKRLPSMKRKAR-GRSIVMPHN-----LFVEMELMFPPHDLLGGKVVKKVKR 103
           RKP+RLPS KRKA  GRSI M HN     + V++EL+FP + L GG++VKKVKR
Sbjct: 62  RKPRRLPSAKRKAGVGRSIFMSHNCLFREMEVDLELLFPSNQLYGGRIVKKVKR 115

BLAST of MS014266 vs. NCBI nr
Match: XP_023532326.1 (cyclin-dependent protein kinase inhibitor SMR14 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 99.8 bits (247), Expect = 1.5e-17
Identity = 60/109 (55.05%), Postives = 77/109 (70.64%), Query Frame = 0

Query: 7   KDKFDILLSTLGVRLPPSV------GEENED-RPEDLKTPTSSEAAAVLQCPPPPRKPKR 66
           +DKF+ILLSTL ++L  S        EE ED R + +KTP S +  AVL+CPPPPRKP+R
Sbjct: 7   EDKFEILLSTLELKLQSSCFRHEDEEEEEEDCRDDFVKTPRSLKPPAVLECPPPPRKPRR 66

Query: 67  LPSMKRKA-RGRSIVMPHN-----LFVEMELMFPPHDLLGGKVVKKVKR 103
           LPSMKRKA  GRSI M H+     + V++EL+FP + L GG++VKKVKR
Sbjct: 67  LPSMKRKAGGGRSIFMSHHCLFGEMEVDLELLFPSNQLHGGRIVKKVKR 115

BLAST of MS014266 vs. NCBI nr
Match: KAG6605463.1 (hypothetical protein SDJN03_02780, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 96.3 bits (238), Expect = 1.7e-16
Identity = 58/115 (50.43%), Postives = 77/115 (66.96%), Query Frame = 0

Query: 2   EEDRLKDKFDILLSTLGVRLPPSV---GEENEDRPED-----LKTPTSSEAAAVLQCPPP 61
           E  + +DKF+ILLSTL ++L  S     +E+E+  ED     +KTP S E  AVL+CPPP
Sbjct: 2   EHHQKEDKFEILLSTLELKLQSSCFRHEDEDEEEEEDCRDDFVKTPRSLEPPAVLECPPP 61

Query: 62  PRKPKRLPSMKRKA-RGRSIVMPHN-----LFVEMELMFPPHDLLGGKVVKKVKR 103
           PRKP+R PS KRKA   RSI M H+     + V++EL+FP + L GG++VKKVKR
Sbjct: 62  PRKPRRFPSAKRKAGDSRSIFMSHHCLFGEMEVDLELLFPSNQLHGGRIVKKVKR 116

BLAST of MS014266 vs. NCBI nr
Match: XP_022947800.1 (cyclin-dependent protein kinase inhibitor SMR10-like [Cucurbita moschata])

HSP 1 Score: 95.5 bits (236), Expect = 2.9e-16
Identity = 57/115 (49.57%), Postives = 75/115 (65.22%), Query Frame = 0

Query: 2   EEDRLKDKFDILLSTLGVRLPPSV--------GEENEDRPEDLKTPTSSEAAAVLQCPPP 61
           E  + +DKF+ILLSTL ++L  S          EE + R + +KTP S E  AVL+CPPP
Sbjct: 2   EHHQKEDKFEILLSTLELKLQSSCFRREDEDEKEEEDCRDDFVKTPRSLEPPAVLECPPP 61

Query: 62  PRKPKRLPSMKRKA-RGRSIVMPHN-----LFVEMELMFPPHDLLGGKVVKKVKR 103
           PRKP+R PS KRKA   RSI M H+     + V++EL+FP + L GG++VKKVKR
Sbjct: 62  PRKPRRFPSAKRKAGDSRSIFMSHHCLFGEMEVDLELLFPSNQLHGGRIVKKVKR 116

BLAST of MS014266 vs. NCBI nr
Match: KAG6570710.1 (hypothetical protein SDJN03_29625, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 89.0 bits (219), Expect = 2.7e-14
Identity = 56/99 (56.57%), Postives = 71/99 (71.72%), Query Frame = 0

Query: 3   EDRLKDKFDILLSTLGVRLP-------PSVGEENED-RPEDL-KTPTSSEAAAVLQCPPP 62
           ED  +DKF+ILLSTL +RLP       P   E+N++ RP+DL KTP S E AA+L+CPPP
Sbjct: 5   EDPKEDKFEILLSTLELRLPLSSFCLRPEEEEDNDEARPDDLVKTPRSLEPAAILECPPP 64

Query: 63  PRKPKRLPSM-KRKARGRSIVMPHN-LFVEM---ELMFP 88
           PRKP+RLPS+ KRKA GR   MPH+ +F EM   E++FP
Sbjct: 65  PRKPRRLPSVRKRKAGGRLTFMPHDYVFGEMKSLEVLFP 103

BLAST of MS014266 vs. ExPASy Swiss-Prot
Match: Q9LTJ2 (Cyclin-dependent protein kinase inhibitor SMR14 OS=Arabidopsis thaliana OX=3702 GN=SMR14 PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 7.6e-04
Identity = 34/79 (43.04%), Postives = 46/79 (58.23%), Query Frame = 0

Query: 28  ENEDRPEDLKTP-TSSEAAAVLQCPP-PPRKPKRLPSMKRKAR--GRSIVMPHNLFVEME 87
           E +D    +K P T   + +V  CPP PPRKPK +PSMKR+     RS+V   ++  E+E
Sbjct: 76  ECKDEESPVKIPETPRNSNSVCYCPPRPPRKPKAIPSMKRRGMWVKRSVVF-LDVAREVE 135

Query: 88  LMFPPHDLLG-GKVVKKVK 102
            MFPP  L   GK +KK +
Sbjct: 136 SMFPPSVLQDFGKKIKKAR 153

BLAST of MS014266 vs. ExPASy TrEMBL
Match: A0A6J1KYS4 (cyclin-dependent protein kinase inhibitor SMR14-like OS=Cucurbita maxima OX=3661 GN=LOC111499465 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.3e-19
Identity = 63/114 (55.26%), Postives = 78/114 (68.42%), Query Frame = 0

Query: 1   EEEDRLKDKFDILLSTLGVRLPPSV-----GEENED-RPEDLKTPTSSEAAAVLQCPPPP 60
           E   + +DKF+ILLSTL ++L  S       EE ED R + +KTP S E A VLQCPPPP
Sbjct: 2   EHHPQKEDKFEILLSTLELKLQSSCFRHEDEEEEEDCRDDFVKTPRSLEPAVVLQCPPPP 61

Query: 61  RKPKRLPSMKRKAR-GRSIVMPHN-----LFVEMELMFPPHDLLGGKVVKKVKR 103
           RKP+RLPS KRKA  GRSI M HN     + V++EL+FP + L GG++VKKVKR
Sbjct: 62  RKPRRLPSAKRKAGVGRSIFMSHNCLFREMEVDLELLFPSNQLYGGRIVKKVKR 115

BLAST of MS014266 vs. ExPASy TrEMBL
Match: A0A6J1G7X1 (cyclin-dependent protein kinase inhibitor SMR10-like OS=Cucurbita moschata OX=3662 GN=LOC111451555 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.4e-16
Identity = 57/115 (49.57%), Postives = 75/115 (65.22%), Query Frame = 0

Query: 2   EEDRLKDKFDILLSTLGVRLPPSV--------GEENEDRPEDLKTPTSSEAAAVLQCPPP 61
           E  + +DKF+ILLSTL ++L  S          EE + R + +KTP S E  AVL+CPPP
Sbjct: 2   EHHQKEDKFEILLSTLELKLQSSCFRREDEDEKEEEDCRDDFVKTPRSLEPPAVLECPPP 61

Query: 62  PRKPKRLPSMKRKA-RGRSIVMPHN-----LFVEMELMFPPHDLLGGKVVKKVKR 103
           PRKP+R PS KRKA   RSI M H+     + V++EL+FP + L GG++VKKVKR
Sbjct: 62  PRKPRRFPSAKRKAGDSRSIFMSHHCLFGEMEVDLELLFPSNQLHGGRIVKKVKR 116

BLAST of MS014266 vs. ExPASy TrEMBL
Match: A0A0A0KHB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G190470 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 8.4e-14
Identity = 60/117 (51.28%), Postives = 79/117 (67.52%), Query Frame = 0

Query: 2   EEDRLKDKFDILLSTLGVRLP---PSV-----GEENEDRPEDL-KTPTSSE-AAAVLQCP 61
           E+ +  +KF+ILLSTL +RLP   PS       E+  DR +DL KTP S E  + VLQCP
Sbjct: 7   EDGKKLEKFEILLSTLELRLPLPLPSFCCLGDKEKEVDRGDDLMKTPRSLEPTSVVLQCP 66

Query: 62  PPPRKPKRLPS-MKRKA---RGRSIVMPHNLF---VEMELMFPPHDLLGGKVVKKVK 102
           PPPRKP+R PS  KRKA    GRSI+M H+ F   VE++ +F P +++GGK+VKK+K
Sbjct: 67  PPPRKPRRFPSAAKRKAGGGGGRSILMSHDYFFRDVELDWLF-PSNVVGGKIVKKIK 122

BLAST of MS014266 vs. ExPASy TrEMBL
Match: A0A6P3Z9D7 (cyclin-dependent protein kinase inhibitor SMR10 OS=Ziziphus jujuba OX=326968 GN=LOC107411099 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 2.3e-11
Identity = 49/111 (44.14%), Postives = 68/111 (61.26%), Query Frame = 0

Query: 8   DKFDILLSTLGVRLP---------PSVGEENEDRPEDLKTPTSSE--AAAVLQCPPPPRK 67
           DKFD+L+S+L +++P             ++N+D  +  KTPTSS+    A L CPP PRK
Sbjct: 75  DKFDVLVSSLKLKIPSVQEFRVKEDDKDDDNKDNNDGFKTPTSSDQKIPATLHCPPAPRK 134

Query: 68  PKRLP--SMKRKARGRSIVMPHNLFVEMELMFPP---HDLLGGKVVKKVKR 103
           PK LP  SMKRKA GR   +  ++  E+E +FPP    DL GGK+ KKV++
Sbjct: 135 PKSLPLSSMKRKAHGRHRQVLLDMSNEIESLFPPVLLADLGGGKIKKKVRK 185

BLAST of MS014266 vs. ExPASy TrEMBL
Match: A0A5N5LFK3 (Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_013780 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.1e-08
Identity = 43/109 (39.45%), Postives = 66/109 (60.55%), Query Frame = 0

Query: 1   EEEDRLKDKFDILLSTLGVRLPP----SVGEENEDRPEDLKTPTS--SEAAAVLQCPPPP 60
           EEE+  +D  ++ + TL ++LP      + ++ +D  +  KTPTS   +   + QCPP P
Sbjct: 252 EEEEEQEDDCEVSVPTLKIKLPSVEAFQIEDDKDDDDDGFKTPTSLDRKIPVIFQCPPAP 311

Query: 61  RKPKRLPSMKRKARGRSIVMPHNLFVEMELMFPPHDL--LGGKVVKKVK 102
           RKPK LPS KRK+  R +++  +L  E+E +FPP     LGGK +KKV+
Sbjct: 312 RKPKSLPSTKRKSAQRRVLL--DLSNEIESLFPPAPAGDLGGK-IKKVR 357

BLAST of MS014266 vs. TAIR 10
Match: AT5G59360.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 44.7 bits (104), Expect = 5.4e-05
Identity = 34/79 (43.04%), Postives = 46/79 (58.23%), Query Frame = 0

Query: 28  ENEDRPEDLKTP-TSSEAAAVLQCPP-PPRKPKRLPSMKRKAR--GRSIVMPHNLFVEME 87
           E +D    +K P T   + +V  CPP PPRKPK +PSMKR+     RS+V   ++  E+E
Sbjct: 76  ECKDEESPVKIPETPRNSNSVCYCPPRPPRKPKAIPSMKRRGMWVKRSVVF-LDVAREVE 135

Query: 88  LMFPPHDLLG-GKVVKKVK 102
            MFPP  L   GK +KK +
Sbjct: 136 SMFPPSVLQDFGKKIKKAR 153

BLAST of MS014266 vs. TAIR 10
Match: AT2G28870.1 (unknown protein; Has 34 Blast hits to 34 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 34; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 42.0 bits (97), Expect = 3.5e-04
Identity = 29/76 (38.16%), Postives = 42/76 (55.26%), Query Frame = 0

Query: 24  SVGEENEDRPEDLKTPTSSE---AAAVLQCPPPPRKPKRLPSMKRKARG-----RSIVMP 83
           +V +E E+  +  KTPT S+   +A    CPP PRKPKR+PS   K R      R I++ 
Sbjct: 73  TVKKEEEEEDDYCKTPTRSDQILSAMPRICPPAPRKPKRVPSRSLKVRNSYRSKRMIIL- 132

Query: 84  HNLFVEMELMFPPHDL 92
            N+  E++ +F P  L
Sbjct: 133 -NVSREIDCLFNPTSL 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023006801.14.7e-1955.26cyclin-dependent protein kinase inhibitor SMR14-like [Cucurbita maxima][more]
XP_023532326.11.5e-1755.05cyclin-dependent protein kinase inhibitor SMR14 [Cucurbita pepo subsp. pepo][more]
KAG6605463.11.7e-1650.43hypothetical protein SDJN03_02780, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022947800.12.9e-1649.57cyclin-dependent protein kinase inhibitor SMR10-like [Cucurbita moschata][more]
KAG6570710.12.7e-1456.57hypothetical protein SDJN03_29625, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q9LTJ27.6e-0443.04Cyclin-dependent protein kinase inhibitor SMR14 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1KYS42.3e-1955.26cyclin-dependent protein kinase inhibitor SMR14-like OS=Cucurbita maxima OX=3661... [more]
A0A6J1G7X11.4e-1649.57cyclin-dependent protein kinase inhibitor SMR10-like OS=Cucurbita moschata OX=36... [more]
A0A0A0KHB18.4e-1451.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G190470 PE=4 SV=1[more]
A0A6P3Z9D72.3e-1144.14cyclin-dependent protein kinase inhibitor SMR10 OS=Ziziphus jujuba OX=326968 GN=... [more]
A0A5N5LFK31.1e-0839.45Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_013780 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59360.15.4e-0543.04unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0... [more]
AT2G28870.13.5e-0438.16unknown protein; Has 34 Blast hits to 34 proteins in 9 species: Archae - 0; Bact... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..68
NoneNo IPR availablePANTHERPTHR33142:SF66CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR2-LIKEcoord: 6..102
IPR040389Cyclin-dependent protein kinase inhibitor SMRPANTHERPTHR33142CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR13coord: 6..102

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014266.1MS014266.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032875 regulation of DNA endoreduplication