MS014220 (gene) Bitter gourd (TR) v1

Overview


Name	MS014220
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ubiquitinyl hydrolase 1
Location	scaffold5: 1464974 .. 1465816 (-)
RNA-Seq Expression	MS014220
Synteny	MS014220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCATGGCGAGCGAGGATTGCCGAGTTTATCACGAGAGGCAAAAGTTACAGTTCTGCCTCTTGCACGCTCTCAATAACCTCTTTCAGGTACCTTTTCATCCGCGAACCTTTGGCTGTGGTGACCGCTCTCTGATTGTTTGAAATGGAAAATTTTGTTAAATCTTGAAATTTATGATTTACGGTGTAATTAAGATGCAGAGAAATGGAAATATTCTTTTTCTCATCACTAAGGAAGAATTATGGTGCTGGGGATCTGAGAAATCGAACTTATCCTCTTACTATTTCACGATTCGTTGCATTCATGCTGTATTTCGATGTCCTCTCACCATTAATACGTTTTCTTTACATCTCAATCAAATACCCCTTGTACAACAGGAAAATGATGCATTCACTCGAGCAAACTTAAATGCAATTGCTGAAAATCTGGTGCTCAACGAGCCAAACAAAGAACCTTGGACGCCTTTATCATTTGTCTTTAAGCCTCATCACAACACGGTAACTGGAAATTATGACATAAATGTTCTAATTTCAGCCCTGGAAGAGAAAGGGAAGAGTGTGGTGTGGCATGACCGTCGGAATGGAGCTTCTTCAATCGATTTAGATGGGCCTGAGGATACTCATAAACTTATGGGAATTGTGCTTAACGTCTCAGTTAGCAGGTTTGGGGGCATTTGGAAAAGTAGACACTGGATTGCACTGAGAAAGATCAACGGATTATGGTATAATTTGGACAGTGATCTGCCCTCTCCTCGCCCATTTAAGGATACTGATGAAGTCAGGAAATTTTTGGATTACGAGATCGGTCATGATGGGGAAATTTTGCTTGTTATGAATGATGGAAAC

mRNA sequence

ACCATGGCGAGCGAGGATTGCCGAGTTTATCACGAGAGGCAAAAGTTACAGTTCTGCCTCTTGCACGCTCTCAATAACCTCTTTCAGGAAAATGATGCATTCACTCGAGCAAACTTAAATGCAATTGCTGAAAATCTGGTGCTCAACGAGCCAAACAAAGAACCTTGGACGCCTTTATCATTTGTCTTTAAGCCTCATCACAACACGGTAACTGGAAATTATGACATAAATGTTCTAATTTCAGCCCTGGAAGAGAAAGGGAAGAGTGTGGTGTGGCATGACCGTCGGAATGGAGCTTCTTCAATCGATTTAGATGGGCCTGAGGATACTCATAAACTTATGGGAATTGTGCTTAACGTCTCAGTTAGCAGGTTTGGGGGCATTTGGAAAAGTAGACACTGGATTGCACTGAGAAAGATCAACGGATTATGGTATAATTTGGACAGTGATCTGCCCTCTCCTCGCCCATTTAAGGATACTGATGAAGTCAGGAAATTTTTGGATTACGAGATCGGTCATGATGGGGAAATTTTGCTTGTTATGAATGATGGAAAC

Coding sequence (CDS)

ACCATGGCGAGCGAGGATTGCCGAGTTTATCACGAGAGGCAAAAGTTACAGTTCTGCCTCTTGCACGCTCTCAATAACCTCTTTCAGGAAAATGATGCATTCACTCGAGCAAACTTAAATGCAATTGCTGAAAATCTGGTGCTCAACGAGCCAAACAAAGAACCTTGGACGCCTTTATCATTTGTCTTTAAGCCTCATCACAACACGGTAACTGGAAATTATGACATAAATGTTCTAATTTCAGCCCTGGAAGAGAAAGGGAAGAGTGTGGTGTGGCATGACCGTCGGAATGGAGCTTCTTCAATCGATTTAGATGGGCCTGAGGATACTCATAAACTTATGGGAATTGTGCTTAACGTCTCAGTTAGCAGGTTTGGGGGCATTTGGAAAAGTAGACACTGGATTGCACTGAGAAAGATCAACGGATTATGGTATAATTTGGACAGTGATCTGCCCTCTCCTCGCCCATTTAAGGATACTGATGAAGTCAGGAAATTTTTGGATTACGAGATCGGTCATGATGGGGAAATTTTGCTTGTTATGAATGATGGAAAC

Protein sequence

TMASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSFVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVSVSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVMNDGN

Homology

[Close]

BLAST of MS014220 vs. NCBI nr
Match: XP_022148354.1 (josephin-like protein [Momordica charantia] >XP_022148355.1 josephin-like protein [Momordica charantia] >XP_022148357.1 josephin-like protein [Momordica charantia] >XP_022148358.1 josephin-like protein [Momordica charantia])

HSP 1 Score: 390.2 bits (1001), Expect = 1.0e-104
Identity = 183/184 (99.46%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 61
           MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 60

Query: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 121
           VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS
Sbjct: 61  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 120

Query: 122 VSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 181
           V RFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM
Sbjct: 121 VRRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 180

Query: 182 NDGN 186
           NDGN
Sbjct: 181 NDGN 184

[Close]

BLAST of MS014220 vs. NCBI nr
Match: XP_038902688.1 (josephin-like protein [Benincasa hispida] >XP_038902689.1 josephin-like protein [Benincasa hispida])

HSP 1 Score: 356.7 bits (914), Expect = 1.2e-94
Identity = 166/184 (90.22%), Postives = 175/184 (95.11%), Query Frame = 0


Query: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 61
           MASEDCRVYHERQKLQFCLLHALNNLFQE DAFTRANLNAIAE LVL+EPN+E WTPLSF
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSF 60

Query: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 121
           VFKPHHNTVTGNYDINVLISALEEKGK VVWHD RNGASSIDLDGPEDTHKLMGIVLNVS
Sbjct: 61  VFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 120

Query: 122 VSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 181
           V RFGGIWKSRHW+ALRKINGLWYNLDSDLP+P PFKDTDEVR+FLD+ IG++GEILL+M
Sbjct: 121 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIM 180

Query: 182 NDGN 186
           ND N
Sbjct: 181 NDRN 184

[Close]

BLAST of MS014220 vs. NCBI nr
Match: XP_022986948.1 (josephin-like protein [Cucurbita maxima])

HSP 1 Score: 345.5 bits (885), Expect = 2.8e-91
Identity = 163/184 (88.59%), Postives = 171/184 (92.93%), Query Frame = 0


Query: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 61
           MASEDCRVYHERQKLQFCLLHALNNLFQEN+AFTRA+LNAIAE LVL+EPNKEPWTPLSF
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQENNAFTRAHLNAIAEKLVLDEPNKEPWTPLSF 60

Query: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 121
           VFKPHHN+VTGNYDINVLISALEEKGKSVVWHD RNGASSIDLDGPE THKLMGIVLNV 
Sbjct: 61  VFKPHHNSVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEATHKLMGIVLNVP 120

Query: 122 VSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 181
           V RFGGIWKSRHW+ALRKIN LWYNLDSDLP+P  FKDTDEVR+FLD  I  +GEILLVM
Sbjct: 121 VRRFGGIWKSRHWVALRKINELWYNLDSDLPTPHTFKDTDEVRQFLDNVINDNGEILLVM 180

Query: 182 NDGN 186
           ND N
Sbjct: 181 NDRN 184

[Close]

BLAST of MS014220 vs. NCBI nr
Match: KAG6570667.1 (Josephin-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 341.7 bits (875), Expect = 4.1e-90
Identity = 161/184 (87.50%), Postives = 170/184 (92.39%), Query Frame = 0


Query: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 61
           MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRA+LNAI+E LVL+EPN+EPWTPLSF
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRAHLNAISEKLVLDEPNREPWTPLSF 60

Query: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 121
           VFKPHHN+VTGNYDINVLISALEEKGKSVVWHD RNGASSIDLDGPE THKLMGIVLNV 
Sbjct: 61  VFKPHHNSVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEATHKLMGIVLNVP 120

Query: 122 VSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 181
           V RFGGIWKSRHW+ALRKIN LWYNLDSDL +P  FKDTDEVR+FLD  I  +GEILLVM
Sbjct: 121 VRRFGGIWKSRHWVALRKINELWYNLDSDLHTPHTFKDTDEVRQFLDNVINDNGEILLVM 180

Query: 182 NDGN 186
           ND N
Sbjct: 181 NDRN 184

[Close]

BLAST of MS014220 vs. NCBI nr
Match: XP_023512266.1 (uncharacterized protein LOC111777056 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 341.7 bits (875), Expect = 4.1e-90
Identity = 161/184 (87.50%), Postives = 170/184 (92.39%), Query Frame = 0


Query: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 61
           MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRA+LNAI+E LVL+EPN+EPWTPLSF
Sbjct: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRAHLNAISEKLVLDEPNREPWTPLSF 61

Query: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 121
           VFKPHHN+VTGNYDINVLISALEEKGKSVVWHD RNGASSIDLDGPE THKLMGIVLNV 
Sbjct: 62  VFKPHHNSVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEATHKLMGIVLNVP 121

Query: 122 VSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 181
           V RFGGIWKSRHW+ALRKIN LWYNLDSDL +P  FKDTDEVR+FLD  I  +GEILLVM
Sbjct: 122 VRRFGGIWKSRHWVALRKINELWYNLDSDLHTPHTFKDTDEVRQFLDNVINDNGEILLVM 181

Query: 182 NDGN 186
           ND N
Sbjct: 182 NDRN 185

[Close]

BLAST of MS014220 vs. ExPASy Swiss-Prot
Match: O82391 (Josephin-like protein OS=Arabidopsis thaliana OX=3702 GN=At2g29640 PE=2 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 1.8e-69
Identity = 119/173 (68.79%), Postives = 142/173 (82.08%), Query Frame = 0


Query: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 61
           MA  + ++YHERQ+LQFCLLH LNNLFQ+ DAFT+ +LN+IAE L  N+PNKE WTPLSF
Sbjct: 1   MADSESKIYHERQRLQFCLLHCLNNLFQDKDAFTKESLNSIAEKLETNDPNKETWTPLSF 60

Query: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 121
           V KPHHNT+TGNYD+NV+I+ALE KGKSVVWHD+R GASSIDLD   D   LMGIVLNV 
Sbjct: 61  VLKPHHNTITGNYDVNVMITALEGKGKSVVWHDKRIGASSIDLD---DADTLMGIVLNVP 120

Query: 122 VSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHD 175
           V R+GG+W+SRHW+ +RKING+WYNLDSDL  P+ F+D DEVR FLD  +  D
Sbjct: 121 VKRYGGLWRSRHWVVVRKINGVWYNLDSDLVVPQLFRDDDEVRGFLDQNLSLD 170

[Close]

BLAST of MS014220 vs. ExPASy Swiss-Prot
Match: Q5BJY4 (Josephin-1 OS=Rattus norvegicus OX=10116 GN=Josd1 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 5.1e-27
Identity = 70/181 (38.67%), Postives = 102/181 (56.35%), Query Frame = 0


Query: 8   RVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSFVFKPHH 67
           ++YHE+Q+ + C LHALNN+FQ+++AFTR  L  I + L    PN         V  PH 
Sbjct: 25  QIYHEKQRRELCALHALNNVFQDSNAFTRETLQEIFQRL---SPNT--------VVTPHK 84

Query: 68  NTV--TGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVSVSRF 127
            ++   GNYD+NV+++AL+ KG   VW D+R     I L        +MG ++N+  S  
Sbjct: 85  KSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIAL------ANVMGFIMNLPSSLC 144

Query: 128 GG----IWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEI-GHDGEILLV 182
            G      K +HWI +R++ G +YNLDS L  P       E+RKFL Y + G + E+LLV
Sbjct: 145 WGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKYHLRGKNCELLLV 188

[Close]

BLAST of MS014220 vs. ExPASy Swiss-Prot
Match: Q9DBJ6 (Josephin-1 OS=Mus musculus OX=10090 GN=Josd1 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 8.7e-27
Identity = 69/181 (38.12%), Postives = 102/181 (56.35%), Query Frame = 0


Query: 8   RVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSFVFKPHH 67
           ++YHE+Q+ + C LHALNN+FQ+++AFTR  L  I + L    PN         +  PH 
Sbjct: 25  QIYHEKQRRELCALHALNNVFQDSNAFTRETLQEIFQRL---SPNT--------MVTPHK 84

Query: 68  NTV--TGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVSVSRF 127
            ++   GNYD+NV+++AL+ KG   VW D+R     I L        +MG ++N+  S  
Sbjct: 85  KSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIALT------NVMGFIMNLPSSLC 144

Query: 128 GG----IWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEI-GHDGEILLV 182
            G      K +HWI +R++ G +YNLDS L  P       E+RKFL Y + G + E+LLV
Sbjct: 145 WGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKYHLRGKNCELLLV 188

[Close]

BLAST of MS014220 vs. ExPASy Swiss-Prot
Match: Q5EAE5 (Josephin-1 OS=Bos taurus OX=9913 GN=JOSD1 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-26
Identity = 68/181 (37.57%), Postives = 103/181 (56.91%), Query Frame = 0


Query: 8   RVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSFVFKPHH 67
           ++YHE+Q+ + C LHALNN+FQ+++AFTR  L  I + L    PN         +  PH 
Sbjct: 25  QIYHEKQRRELCALHALNNVFQDSNAFTRETLQEIFQRL---SPNT--------MVTPHK 84

Query: 68  NTV--TGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVSVSRF 127
            ++   GNYD+NV+++AL+ KG   VW D+R    +I L        +MG ++N+  S  
Sbjct: 85  KSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGAIALT------NVMGFIMNLPSSLC 144

Query: 128 GG----IWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEI-GHDGEILLV 182
            G      K +HWI +R++ G +YNLDS L  P       E+RKFL + + G + E+LLV
Sbjct: 145 WGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGKSELRKFLKHHLRGKNCELLLV 188

[Close]

BLAST of MS014220 vs. ExPASy Swiss-Prot
Match: Q15040 (Josephin-1 OS=Homo sapiens OX=9606 GN=JOSD1 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 4.3e-26
Identity = 68/181 (37.57%), Postives = 102/181 (56.35%), Query Frame = 0


Query: 8   RVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSFVFKPHH 67
           ++YHE+Q+ + C LHALNN+FQ+++AFTR  L  I + L    PN         +  PH 
Sbjct: 25  QIYHEKQRRELCALHALNNVFQDSNAFTRDTLQEIFQRL---SPNT--------MVTPHK 84

Query: 68  NTV--TGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVSVSRF 127
            ++   GNYD+NV+++AL+ KG   VW D+R     I L        +MG ++N+  S  
Sbjct: 85  KSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIALT------NVMGFIMNLPSSLC 144

Query: 128 GG----IWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEI-GHDGEILLV 182
            G      K +HWI +R++ G +YNLDS L  P       E+RKFL + + G + E+LLV
Sbjct: 145 WGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKHHLRGKNCELLLV 188

[Close]

BLAST of MS014220 vs. ExPASy TrEMBL
Match: A0A6J1D2P2 (Ubiquitinyl hydrolase 1 OS=Momordica charantia OX=3673 GN=LOC111017028 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 4.8e-105
Identity = 183/184 (99.46%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 61
           MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 60

Query: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 121
           VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS
Sbjct: 61  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 120

Query: 122 VSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 181
           V RFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM
Sbjct: 121 VRRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 180

Query: 182 NDGN 186
           NDGN
Sbjct: 181 NDGN 184

[Close]

BLAST of MS014220 vs. ExPASy TrEMBL
Match: A0A6J1JI12 (Ubiquitinyl hydrolase 1 OS=Cucurbita maxima OX=3661 GN=LOC111484533 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.4e-91
Identity = 163/184 (88.59%), Postives = 171/184 (92.93%), Query Frame = 0


Query: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 61
           MASEDCRVYHERQKLQFCLLHALNNLFQEN+AFTRA+LNAIAE LVL+EPNKEPWTPLSF
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQENNAFTRAHLNAIAEKLVLDEPNKEPWTPLSF 60

Query: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 121
           VFKPHHN+VTGNYDINVLISALEEKGKSVVWHD RNGASSIDLDGPE THKLMGIVLNV 
Sbjct: 61  VFKPHHNSVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEATHKLMGIVLNVP 120

Query: 122 VSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 181
           V RFGGIWKSRHW+ALRKIN LWYNLDSDLP+P  FKDTDEVR+FLD  I  +GEILLVM
Sbjct: 121 VRRFGGIWKSRHWVALRKINELWYNLDSDLPTPHTFKDTDEVRQFLDNVINDNGEILLVM 180

Query: 182 NDGN 186
           ND N
Sbjct: 181 NDRN 184

[Close]

BLAST of MS014220 vs. ExPASy TrEMBL
Match: A0A6J1FYW5 (Ubiquitinyl hydrolase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449101 PE=4 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.7e-89
Identity = 159/184 (86.41%), Postives = 169/184 (91.85%), Query Frame = 0


Query: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 61
           MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRA+LNAI+E LVL+EPN+EPWTPLSF
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRAHLNAISEKLVLDEPNREPWTPLSF 60

Query: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 121
           VFKPHHN+VTGNYDINVLISA+EEKGKSVVWHD RNGASSIDLDGPE THKLMGIVLNV 
Sbjct: 61  VFKPHHNSVTGNYDINVLISAMEEKGKSVVWHDHRNGASSIDLDGPEATHKLMGIVLNVP 120

Query: 122 VSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 181
           V RFGGIWKSRHW+ALRKIN  WYNLDSDL +P  FKDTDEVR+FLD  I  +GEILLVM
Sbjct: 121 VRRFGGIWKSRHWVALRKINESWYNLDSDLHTPHTFKDTDEVRQFLDNVINDNGEILLVM 180

Query: 182 NDGN 186
           ND N
Sbjct: 181 NDRN 184

[Close]

BLAST of MS014220 vs. ExPASy TrEMBL
Match: A0A1S4E4I3 (Ubiquitinyl hydrolase 1 OS=Cucumis melo OX=3656 GN=LOC103502098 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.4e-88
Identity = 156/184 (84.78%), Postives = 167/184 (90.76%), Query Frame = 0


Query: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 61
           M  EDC VYHERQKLQ CLLHALNNLFQE DAFTRANLNAIAE LV +EPN+E WTPLS 
Sbjct: 2   MVREDCGVYHERQKLQLCLLHALNNLFQEKDAFTRANLNAIAEKLVRDEPNQETWTPLSI 61

Query: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 121
           VFKPHHNTVTGNYDINVLISALEEKGKSVVWHD RNGAS+IDLDGP+DT KLMGIVLNVS
Sbjct: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASTIDLDGPDDTRKLMGIVLNVS 121

Query: 122 VSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 181
           V RFGGIWKSRHW+ALRKI+GLWYNLDSDLP+P PFKDT+EVR+FLD+ I  +GEILLVM
Sbjct: 122 VRRFGGIWKSRHWVALRKIDGLWYNLDSDLPAPHPFKDTEEVREFLDHVINDNGEILLVM 181

Query: 182 NDGN 186
           ND N
Sbjct: 182 NDRN 185

[Close]

BLAST of MS014220 vs. ExPASy TrEMBL
Match: A0A5D3CVY1 (Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00510 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.4e-88
Identity = 156/184 (84.78%), Postives = 167/184 (90.76%), Query Frame = 0


Query: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 61
           M  EDC VYHERQKLQ CLLHALNNLFQE DAFTRANLNAIAE LV +EPN+E WTPLS 
Sbjct: 2   MVREDCGVYHERQKLQLCLLHALNNLFQEKDAFTRANLNAIAEKLVRDEPNQETWTPLSI 61

Query: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 121
           VFKPHHNTVTGNYDINVLISALEEKGKSVVWHD RNGAS+IDLDGP+DT KLMGIVLNVS
Sbjct: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASTIDLDGPDDTRKLMGIVLNVS 121

Query: 122 VSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 181
           V RFGGIWKSRHW+ALRKI+GLWYNLDSDLP+P PFKDT+EVR+FLD+ I  +GEILLVM
Sbjct: 122 VRRFGGIWKSRHWVALRKIDGLWYNLDSDLPAPHPFKDTEEVREFLDHVINDNGEILLVM 181

Query: 182 NDGN 186
           ND N
Sbjct: 182 NDRN 185

[Close]

BLAST of MS014220 vs. TAIR 10
Match: AT2G29640.1 (JOSEPHIN-like protein )

HSP 1 Score: 263.5 bits (672), Expect = 1.3e-70
Identity = 119/173 (68.79%), Postives = 142/173 (82.08%), Query Frame = 0


Query: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 61
           MA  + ++YHERQ+LQFCLLH LNNLFQ+ DAFT+ +LN+IAE L  N+PNKE WTPLSF
Sbjct: 1   MADSESKIYHERQRLQFCLLHCLNNLFQDKDAFTKESLNSIAEKLETNDPNKETWTPLSF 60

Query: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 121
           V KPHHNT+TGNYD+NV+I+ALE KGKSVVWHD+R GASSIDLD   D   LMGIVLNV 
Sbjct: 61  VLKPHHNTITGNYDVNVMITALEGKGKSVVWHDKRIGASSIDLD---DADTLMGIVLNVP 120

Query: 122 VSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHD 175
           V R+GG+W+SRHW+ +RKING+WYNLDSDL  P+ F+D DEVR FLD  +  D
Sbjct: 121 VKRYGGLWRSRHWVVVRKINGVWYNLDSDLVVPQLFRDDDEVRGFLDQNLSLD 170

[Close]

BLAST of MS014220 vs. TAIR 10
Match: AT3G54130.1 (Josephin family protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.6e-07
Identity = 42/155 (27.10%), Postives = 69/155 (44.52%), Query Frame = 0


Query: 9   VYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENL-------VLNEPNKEPWTPLSF 68
           +YHE Q+   C +H +N + Q    F+  +L A+A +L       +L       + P  F
Sbjct: 10  LYHEVQESNLCAVHCVNTVLQ-GPFFSEFDLAAVAADLDGKERQVMLEGAAVGGFAPGDF 69

Query: 69  VFKPHHN-TVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNV 128
           + +  HN ++ G++ I VL  ALE      VW  +    +  D +  +   +L    +  
Sbjct: 70  LAEESHNVSLGGDFSIQVLQKALE------VWDLQVIPLNCPDAEPAQIDPELESAFI-- 129

Query: 129 SVSRFGGIWKSRHWIALRKINGLWYNLDSDLPSPR 156
                       HW  +RK+NG WYN DS L +P+
Sbjct: 130 -------CHLHDHWFCIRKVNGEWYNFDSLLAAPQ 148

The following BLAST results are available for this feature:

BLAST of MS014220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148354.1	1.0e-104	99.46	josephin-like protein [Momordica charantia] >XP_022148355.1 josephin-like protei...	[more]
XP_038902688.1	1.2e-94	90.22	josephin-like protein [Benincasa hispida] >XP_038902689.1 josephin-like protein ...	[more]
XP_022986948.1	2.8e-91	88.59	josephin-like protein [Cucurbita maxima]	[more]
KAG6570667.1	4.1e-90	87.50	Josephin-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023512266.1	4.1e-90	87.50	uncharacterized protein LOC111777056 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS014220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82391	1.8e-69	68.79	Josephin-like protein OS=Arabidopsis thaliana OX=3702 GN=At2g29640 PE=2 SV=1	[more]
Q5BJY4	5.1e-27	38.67	Josephin-1 OS=Rattus norvegicus OX=10116 GN=Josd1 PE=2 SV=1	[more]
Q9DBJ6	8.7e-27	38.12	Josephin-1 OS=Mus musculus OX=10090 GN=Josd1 PE=1 SV=1	[more]
Q5EAE5	1.5e-26	37.57	Josephin-1 OS=Bos taurus OX=9913 GN=JOSD1 PE=2 SV=1	[more]
Q15040	4.3e-26	37.57	Josephin-1 OS=Homo sapiens OX=9606 GN=JOSD1 PE=1 SV=1	[more]

BLAST of MS014220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2P2	4.8e-105	99.46	Ubiquitinyl hydrolase 1 OS=Momordica charantia OX=3673 GN=LOC111017028 PE=4 SV=1	[more]
A0A6J1JI12	1.4e-91	88.59	Ubiquitinyl hydrolase 1 OS=Cucurbita maxima OX=3661 GN=LOC111484533 PE=4 SV=1	[more]
A0A6J1FYW5	1.7e-89	86.41	Ubiquitinyl hydrolase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449101 PE=4 SV=1	[more]
A0A1S4E4I3	2.4e-88	84.78	Ubiquitinyl hydrolase 1 OS=Cucumis melo OX=3656 GN=LOC103502098 PE=4 SV=1	[more]
A0A5D3CVY1	2.4e-88	84.78	Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of MS014220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29640.1	1.3e-70	68.79	JOSEPHIN-like protein	[more]
AT3G54130.1	1.6e-07	27.10	Josephin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006155	Josephin domain	SMART	SM01246	Josephin_2	coord: 13..183 e-value: 6.7E-66 score: 234.9
IPR006155	Josephin domain	PFAM	PF02099	Josephin	coord: 14..180 e-value: 9.9E-36 score: 123.2
IPR006155	Josephin domain	PROSITE	PS50957	JOSEPHIN	coord: 6..185 score: 48.342297
None	No IPR available	GENE3D	3.90.70.40		coord: 18..185 e-value: 4.7E-25 score: 89.7
None	No IPR available	PANTHER	PTHR13291:SF0	JOSEPHIN-LIKE PROTEIN	coord: 2..183
IPR040053	Josephin-1/2	PANTHER	PTHR13291	JOSEPHIN 1, 2	coord: 2..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS014220.1	MS014220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016579	protein deubiquitination
molecular_function	GO:0004843	thiol-dependent deubiquitinase