MS014219 (gene) Bitter gourd (TR) v1

Overview
NameMS014219
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionJosephin-like protein
Locationscaffold5: 1463078 .. 1463395 (-)
RNA-Seq ExpressionMS014219
SyntenyMS014219
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGACAACGGCAAAGCAGAGGATTGAGAAACAGAACTCGACGAGCAACGGAACAGGGCGAAACGGAGGTTCGTCTTTTGGGAAGTGCAGATTTAAACCCCCCCTTAAACGGTTGCAATTCTCTCCTCTGAGCTTTCTGAAGCACCTGGCCGATAAAGTGACCCGAGCTTTGCATTTGATGTCCATGAAGAGAAGACCCTGCCATGAACAACCCGCACCAGCCGTGCCCGCCGCCAGTTCAAAGCCAGTCTCCGCCATCGATTCACATCGAACAGAGGCTGTGGAGGACTGCATCAAATACATCAACTCTTCTTCT

mRNA sequence

ATGACGACAACGGCAAAGCAGAGGATTGAGAAACAGAACTCGACGAGCAACGGAACAGGGCGAAACGGAGGTTCGTCTTTTGGGAAGTGCAGATTTAAACCCCCCCTTAAACGGTTGCAATTCTCTCCTCTGAGCTTTCTGAAGCACCTGGCCGATAAAGTGACCCGAGCTTTGCATTTGATGTCCATGAAGAGAAGACCCTGCCATGAACAACCCGCACCAGCCGTGCCCGCCGCCAGTTCAAAGCCAGTCTCCGCCATCGATTCACATCGAACAGAGGCTGTGGAGGACTGCATCAAATACATCAACTCTTCTTCT

Coding sequence (CDS)

ATGACGACAACGGCAAAGCAGAGGATTGAGAAACAGAACTCGACGAGCAACGGAACAGGGCGAAACGGAGGTTCGTCTTTTGGGAAGTGCAGATTTAAACCCCCCCTTAAACGGTTGCAATTCTCTCCTCTGAGCTTTCTGAAGCACCTGGCCGATAAAGTGACCCGAGCTTTGCATTTGATGTCCATGAAGAGAAGACCCTGCCATGAACAACCCGCACCAGCCGTGCCCGCCGCCAGTTCAAAGCCAGTCTCCGCCATCGATTCACATCGAACAGAGGCTGTGGAGGACTGCATCAAATACATCAACTCTTCTTCT

Protein sequence

MTTTAKQRIEKQNSTSNGTGRNGGSSFGKCRFKPPLKRLQFSPLSFLKHLADKVTRALHLMSMKRRPCHEQPAPAVPAASSKPVSAIDSHRTEAVEDCIKYINSSS
Homology
BLAST of MS014219 vs. NCBI nr
Match: XP_038902310.1 (uncharacterized protein LOC120088944 [Benincasa hispida])

HSP 1 Score: 140.2 bits (352), Expect = 1.0e-29
Identity = 80/110 (72.73%), Postives = 91/110 (82.73%), Query Frame = 0

Query: 1   MTTTAKQRIEKQNSTSNGTGRNGGSSFGKCRFKPP--LKRLQFSPLSFLKHLADKVTRAL 60
           MTTT +  I+KQNS+SN T R G  +F KCRF+ P  LK LQFSPLSFLK LAD+V RAL
Sbjct: 1   MTTTLE--IDKQNSSSNKTER-GRGAFQKCRFRSPKSLKGLQFSPLSFLKCLADRVARAL 60

Query: 61  HLMSMKRRPCHEQPAPAVPAASSKPV--SAIDSHRTEAVEDCIKYINSSS 107
           HLM+MKRRPCH+QPAPAVP  SSKP+  SAIDSHRTEAVEDCIKYIN+S+
Sbjct: 61  HLMAMKRRPCHKQPAPAVPVVSSKPIQASAIDSHRTEAVEDCIKYINAST 107

BLAST of MS014219 vs. NCBI nr
Match: KAG6570666.1 (hypothetical protein SDJN03_29581, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 135.2 bits (339), Expect = 3.3e-28
Identity = 80/108 (74.07%), Postives = 89/108 (82.41%), Query Frame = 0

Query: 2   TTTAKQRIEKQNSTSNGTGRNGGSSFGKCRFKPPLKRLQFSPLSFLKHLADKVTRALHLM 61
           T T +QRIEK+N +SN T R+ G SF K RFK  LK LQFSPLSFLKHLA+KVTRA HLM
Sbjct: 3   TATMEQRIEKRNMSSNATRRSTG-SFRKLRFK-SLKGLQFSPLSFLKHLANKVTRAWHLM 62

Query: 62  SMKRRPCHE-QPAPAVPAASSK--PVSAIDSHRTEAVEDCIKYINSSS 107
           +MKRRP HE QP PA+P A+SK  PVSA+DSHRTEAVEDCIKYINSSS
Sbjct: 63  AMKRRPHHEQQPVPAMPVATSKPLPVSAVDSHRTEAVEDCIKYINSSS 108

BLAST of MS014219 vs. NCBI nr
Match: KAG7010513.1 (Josephin-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 130.6 bits (327), Expect = 8.2e-27
Identity = 77/102 (75.49%), Postives = 85/102 (83.33%), Query Frame = 0

Query: 8   RIEKQNSTSNGTGRNGGSSFGKCRFKPPLKRLQFSPLSFLKHLADKVTRALHLMSMKRRP 67
           RIEK+N +SN T R+ G SF K RFK  LK LQFSPLSFLKHLA+KVTRA HLM+MKRRP
Sbjct: 195 RIEKRNMSSNATRRSTG-SFRKLRFK-SLKGLQFSPLSFLKHLANKVTRAWHLMAMKRRP 254

Query: 68  CHE-QPAPAVPAASSK--PVSAIDSHRTEAVEDCIKYINSSS 107
            HE QP PA+P A+SK  PVSA+DSHRTEAVEDCIKYINSSS
Sbjct: 255 HHEQQPVPAMPVATSKPLPVSAVDSHRTEAVEDCIKYINSSS 294

BLAST of MS014219 vs. NCBI nr
Match: XP_023512266.1 (uncharacterized protein LOC111777056 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 130.6 bits (327), Expect = 8.2e-27
Identity = 77/102 (75.49%), Postives = 85/102 (83.33%), Query Frame = 0

Query: 8   RIEKQNSTSNGTGRNGGSSFGKCRFKPPLKRLQFSPLSFLKHLADKVTRALHLMSMKRRP 67
           RIEK+N +SN T R+ G SF K RFK  LK LQFSPLSFLKHLA+KVTRA HLM+MKRRP
Sbjct: 266 RIEKRNMSSNATRRSTG-SFRKLRFK-SLKGLQFSPLSFLKHLANKVTRAWHLMAMKRRP 325

Query: 68  CHE-QPAPAVPAASSK--PVSAIDSHRTEAVEDCIKYINSSS 107
            HE QP PA+P A+SK  PVSA+DSHRTEAVEDCIKYINSSS
Sbjct: 326 HHEQQPVPAMPVATSKPLPVSAVDSHRTEAVEDCIKYINSSS 365

BLAST of MS014219 vs. NCBI nr
Match: KAA0061847.1 (josephin-like protein [Cucumis melo var. makuwa] >TYK15409.1 josephin-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 129.0 bits (323), Expect = 2.4e-26
Identity = 73/111 (65.77%), Postives = 87/111 (78.38%), Query Frame = 0

Query: 2   TTTAKQRIEKQNSTSNGTGRNGGSSFGKCRFKPP--LKRLQFSPLSFLKHLADKVTRALH 61
           TTT +Q+++K NS+SN   R G S+F KCRF+ P  LK LQFSPL FLKHLADKVTRALH
Sbjct: 3   TTTLEQKVKKPNSSSN-NNRTGRSTFQKCRFRSPKSLKGLQFSPLCFLKHLADKVTRALH 62

Query: 62  LMSMKR-RPCHEQPAPAVPAASSKPV---SAIDSHRTEAVEDCIKYINSSS 107
            M+MKR RP  +QP PAVP ASSK +   + +DSHRTEAVEDCIKYIN+S+
Sbjct: 63  FMAMKRNRPLQKQPVPAVPLASSKAIQVPATVDSHRTEAVEDCIKYINAST 112

BLAST of MS014219 vs. ExPASy TrEMBL
Match: A0A5A7V4L5 (Josephin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00520 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.2e-26
Identity = 73/111 (65.77%), Postives = 87/111 (78.38%), Query Frame = 0

Query: 2   TTTAKQRIEKQNSTSNGTGRNGGSSFGKCRFKPP--LKRLQFSPLSFLKHLADKVTRALH 61
           TTT +Q+++K NS+SN   R G S+F KCRF+ P  LK LQFSPL FLKHLADKVTRALH
Sbjct: 3   TTTLEQKVKKPNSSSN-NNRTGRSTFQKCRFRSPKSLKGLQFSPLCFLKHLADKVTRALH 62

Query: 62  LMSMKR-RPCHEQPAPAVPAASSKPV---SAIDSHRTEAVEDCIKYINSSS 107
            M+MKR RP  +QP PAVP ASSK +   + +DSHRTEAVEDCIKYIN+S+
Sbjct: 63  FMAMKRNRPLQKQPVPAVPLASSKAIQVPATVDSHRTEAVEDCIKYINAST 112

BLAST of MS014219 vs. ExPASy TrEMBL
Match: A0A0A0KE45 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G187920 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.3e-25
Identity = 76/113 (67.26%), Postives = 87/113 (76.99%), Query Frame = 0

Query: 1   MTTTAKQRIEKQNSTSNGTGRNGGSSFGKCRFKPP--LKRLQFSPLSFLKHLADKVTRAL 60
           MTTT +Q+++K NS+SN   R G S+F KCRF  P  LKRLQFSPL FLKHLADKVTRAL
Sbjct: 1   MTTTLEQKVKKPNSSSN--NRTGRSTFQKCRFPSPKSLKRLQFSPLCFLKHLADKVTRAL 60

Query: 61  HLMSMKR-RPCHEQPAP-AVPAASSKPV---SAIDSHRTEAVEDCIKYINSSS 107
           H M+MKR RP  +Q  P AVP ASSK V   + IDSHRTEAVEDCIKYIN+S+
Sbjct: 61  HFMAMKRNRPLQKQHVPAAVPVASSKAVQVPATIDSHRTEAVEDCIKYINAST 111

BLAST of MS014219 vs. ExPASy TrEMBL
Match: A0A6A3B894 (Uncharacterized protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00110290pilonHSYRG00003 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 5.7e-10
Identity = 44/96 (45.83%), Postives = 61/96 (63.54%), Query Frame = 0

Query: 12  QNSTSNGTGRNGGSSFGKCRFKPPLKRLQFSPLSFLKHLADKVTRALHLMSMKRRPCHEQ 71
           Q S +   G  G S    C F    KRL+FSP+ FL+HL  KV++ LH +SMK RP H+ 
Sbjct: 19  QKSNTGAAGNKGISGSWSCGFM-LRKRLKFSPVRFLRHLGGKVSKVLHEVSMKIRPSHKV 78

Query: 72  PAPAVPAASSKP-VSAIDSHRTEAVEDCIKYINSSS 107
            +    +  SKP V+ +D+HRT A+EDCI++INSS+
Sbjct: 79  SS---SSGRSKPFVTTVDAHRTAAIEDCIEFINSSA 110

BLAST of MS014219 vs. ExPASy TrEMBL
Match: A0A6A1VD78 (Uncharacterized protein OS=Morella rubra OX=262757 GN=CJ030_MR6G009091 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 8.3e-09
Identity = 44/97 (45.36%), Postives = 59/97 (60.82%), Query Frame = 0

Query: 12  QNSTSNGTGRNGGSSFGKCRFKPPLKRLQFSPLSFLKHLADKVTRALHLMSMKRRPCHEQ 71
           + + S     N G +   CRFK P  +    P+ FLKH+ADK+ RALH  S++R     Q
Sbjct: 11  ERNYSKRVSANSGFARQYCRFKLP--KGPELPIRFLKHIADKIARALHSASLRR----SQ 70

Query: 72  PAPAVPAASSKPVSA--IDSHRTEAVEDCIKYINSSS 107
            A      SSKP+ A  +DSHRTEA+EDCI+++NSSS
Sbjct: 71  SAKDSSFGSSKPIVAMTVDSHRTEAIEDCIEFLNSSS 101

BLAST of MS014219 vs. ExPASy TrEMBL
Match: A0A7J9KLA2 (Uncharacterized protein OS=Gossypium schwendimanii OX=34291 GN=Goshw_013875 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.1e-08
Identity = 46/107 (42.99%), Postives = 64/107 (59.81%), Query Frame = 0

Query: 4   TAKQRIEKQNST---SNGTGRNGGSSFGKCRFKPPLKRLQFSPLSFLKHLADKVTRALHL 63
           T    I K N++   + G  R+    F +C      KR +FSP+ FLKHL  K+ R LH+
Sbjct: 14  TGSTIIHKSNTSAAGNKGVPRSRSYGFMRC------KRSKFSPVRFLKHLGGKLARGLHV 73

Query: 64  MSMKRRPCHEQPAPAVPAASSKP-VSAIDSHRTEAVEDCIKYINSSS 107
           +SMK R   + P  +  +  SKP V+ +DSHRT AVEDCI++INSS+
Sbjct: 74  VSMKIR---QSPKVSSSSGRSKPFVTPVDSHRTAAVEDCIEFINSSA 111

BLAST of MS014219 vs. TAIR 10
Match: AT1G07300.1 (josephin protein-related )

HSP 1 Score: 43.1 bits (100), Expect = 1.6e-04
Identity = 32/99 (32.32%), Postives = 52/99 (52.53%), Query Frame = 0

Query: 11  KQNSTSNGTGRNGGSSFGKCRFKPPLKRLQFSPLSFLKHLADKVTRALHLMSM-KRRPCH 70
           KQ +     G++ G +   C  + P ++ + +    +KHL  K  + L L+ M K++   
Sbjct: 23  KQTAIKGPYGKSPGCT-TSCGLRLP-RKTEVTAAKLIKHLGCKFAKGLRLVVMRKKKRSP 82

Query: 71  EQPAPAVPAASSKPVSAI--DSHRTEAVEDCIKYINSSS 107
                +    S  P+  I  DSHR+EA+EDCI++INSSS
Sbjct: 83  PSKVSSFSGRSQPPIIPINNDSHRSEAIEDCIQFINSSS 119

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902310.11.0e-2972.73uncharacterized protein LOC120088944 [Benincasa hispida][more]
KAG6570666.13.3e-2874.07hypothetical protein SDJN03_29581, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7010513.18.2e-2775.49Josephin-like protein [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023512266.18.2e-2775.49uncharacterized protein LOC111777056 [Cucurbita pepo subsp. pepo][more]
KAA0061847.12.4e-2665.77josephin-like protein [Cucumis melo var. makuwa] >TYK15409.1 josephin-like prote... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7V4L51.2e-2665.77Josephin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold57... [more]
A0A0A0KE451.3e-2567.26Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G187920 PE=4 SV=1[more]
A0A6A3B8945.7e-1045.83Uncharacterized protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00110290pilon... [more]
A0A6A1VD788.3e-0945.36Uncharacterized protein OS=Morella rubra OX=262757 GN=CJ030_MR6G009091 PE=4 SV=1[more]
A0A7J9KLA21.1e-0842.99Uncharacterized protein OS=Gossypium schwendimanii OX=34291 GN=Goshw_013875 PE=4... [more]
Match NameE-valueIdentityDescription
AT1G07300.11.6e-0432.32josephin protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..88
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availablePANTHERPTHR34355JOSEPHIN-LIKE PROTEINcoord: 5..106
NoneNo IPR availablePANTHERPTHR34355:SF7JOSEPHIN PROTEIN-LIKE PROTEINcoord: 5..106

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014219.1MS014219.1mRNA