MS014175 (gene) Bitter gourd (TR) v1

Overview
NameMS014175
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionF-box/LRR-repeat protein 3-like
Locationscaffold5: 1167125 .. 1170052 (-)
RNA-Seq ExpressionMS014175
SyntenyMS014175
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCTCCAACTTTGATTCTGCTCCATCTCTGAAAAACCTCAAGACCATTGACATGGATTGCCCATCTTCCGGCCCCTTTGATCATCTCACGGAGGAGATCGTGTTCTTGATTGTTGGCCATCTTCACGACGATCCCTTTTCTCGGAAATCCTTCTCTCTCGTCTGCAAGTCCTTCCACGCCATCGAATCGCGCCAGCGGAAATCTCTCAGGCCGCTGCGCTCCGATTTGACTCCAAGTATAGCCCCGCGCTTCCCTTCCATTTCGAAGCTCGACCTTTCCCTTTGCCATCACGTGGAGGATACATTTCTGATTTCGATATCGCGTGCTTGGAAAATGACGCTGCGTTCTGTGGATCTATCCCGCTCTAGATCGTTTACCACTGTTGGATTGTCGAGCTTGGTTTCGAATTGTGCTGGACTGGTTGAGATTGACTTGTCGAACGGGGTGGCTCTGACGGACTCCGCGTTGAAGGTCATAGCAGAGGCCAAAAACTTGGAGAAATTGTGGTTGGCGAGGTGCAAATCGATTACGGATATGGGGATTGGTTGTGTGGCTGTTGGATGTAGAAAGTTGAAACACTTGTGCTTGAATTGGTGCCTTCGTATTACTGATTTGGGGGTTGATTTGATTGGAAGGAAGTGCCATGAGCTAAGGAGTTTGGACCTCTCTTTCTTACCGGTACTAAATTCCTTCCTCCTCCTCTCATGTTCTTCCCTCTCTTTCGAAGCTAATGAATATTTTTGCTATAGATCACTGAGAAATGTCTTTCGACTATTCTTCAACTGCAACAACTTGAGGAGCTAGTCCTCGAGGGTTGCCATGGAATCGATGATGGACTTGCAGTCCTAGAACGTCACTGCAAGAGCAACTCATTGAAGGTACATTCTCTCGGTACCTATGTTATACTGTCAGTATGACCTTTTCCTCTGATGGAGCAATATTCCTCTGCAGTTTAAACGTAATTTCTATTGACATAAGTGCAAAATTGTTGGCAGTTGCTTAATCTGTCTCACTGTAAAAGTATAAGTCATATTGGTTTGTCTTCTTTGATAAATGGCGCTCAAGACCTTCAACAGCTAATCTTATCATATGGCTCTTCAGTAAGTGTGGAATACTGTTATGGTTGTATTTGGTGATTATTTGAGATTCTTCTGCTTATATAGGTTATTGTTGCCTTAGGTCACAACAGACGTGGCAAAATGTCTGCATAGTTTTTCGGGTTTGCAATCGATTAAATTAGATTGTTGTTCACTCACCTCTTCTGGGGTTAGAGCCATTGCAAATTGGCGTGCATCACTCAAGGAGCTGAGCTTAAGCAAGTGCTCGGGCGTGATGGACGAGTGCCTTTCCATCCTTGTGCAAAAACACAAGCAGTTGAGAAAATTAGACATCACGTGCTGCCGTAAGATAACTTTTGCATCCATTAACAGCATTACAAGTTCGTGTTCCTCCCTCGTTTCTCTTAAGATGGAATCCTGTAGCCTTGTTCCTAGAGAGGCTTATGTCTTGATAGGAGAACGCTGTCCATTCTTGGAGGAACTTGATGTCACTGACAATGAGATTGACAATGAAGGTTAAACAGGATTTCAGCTCTCCTTTTACATTACATAATGCATGAAGCTCTTCTGTTAATAACATTAAAGTTGCTTCTTTTGTTTAGGTTTGAAGTCAGTCTCAAAATGTTCAAGGCTTAGTGTTCTAAAGCTGGGGATTTGCTCGAATATAAATGATGATGGTCTATTGCACATTGCAAGTGGTTGTCCAAAAATCAAAGAGCTTGATTTGTACAGGTTTGATGTCAACCTTCAGAATCCTCTGTTTGAGTTTTAGGGAGCTCGAGGGAATTAGCTTCCTACCTTGATAGAAGTTTAGTAAGGGAGCATCTGCTTTTGGGATTCACATATTCAAGATTGTTTGTCAAGAATGAATAAAATTTCTTCACTATCACCTGTTATTGCTTATCTCAGTCTTCGAGTGCGCCCCTATAGATATTTTGCTCTTAAAAAGCTTCTGCATCTTTTCACATTTTCTAAGCTGAATAGTCACTAATGTAGATCTATAGATTGATGTTTTGATTGTAAACTGCAGGCTGGTTATTTAATCATTACCCAAAAGAAAATTTTTAAGTACTATAACGTTGTAGGAGCTAGAATAAAGACTAGAAATGTACTCCAACTGAATTGTCTATTGTGCTGGGTTAAGCCCTTTTTGAAGATTTAGAACATGTTTTTCCTGTAATAATGTTTAAGTTATGTCTTTTTCCTTTTTTTTTTTTTTGGGTTAGGTCAACCGGAATAACAGACAGAGGCATTGCAGCAATCGCTGGTGGTTGCCCTGCTCTTGAGATGATTAACATTGCTTATAACATTAAAGTTACAGATTCTTCGTTAATATCATTGTCAAAATGTTTGAGGTTAAAAGCACTTGAAATTCGAGGCTGTTGTTGCATTTCATCAGTTGGCCTCTCCGCCATAGCAATGGGATGTAAGCAACTTACTGTGCTGGATATAAAGAAATGTGTCAATGTAAATGATGATGGGATGCTTCCACTTGCCCGATTCTCCCACAACCTCAAGCAGGTATAGTCCTATATTCTCCATTGATGAGCAAAATGTGGTTGGTGTAGATCATACCGTTAAAGATTTTCCTCTTTTGGTGCAGATAAACTTGTCGTACTGTTCAGTTACAGATGTTGGGCTATTGTCGCTAGCAAGCATTAATTGCCTGAGGAACATGACCATCTTGCATTTGGCTGGCTTGACTCCGAATGGCCTCACTGCCGCCCTGCTGGTGTGTGGTACTCTACGAAAGGTAAAGCTGCATCTGTCATTCAAATCCAAGCTTCCTCCATCTTTCTCCAAGTACATGGAATCCCATGGATGCGTATTGTACTGGAGGGACAAAGCATTCCAGGTACTCATTTTC

mRNA sequence

ATGTTCTCCAACTTTGATTCTGCTCCATCTCTGAAAAACCTCAAGACCATTGACATGGATTGCCCATCTTCCGGCCCCTTTGATCATCTCACGGAGGAGATCGTGTTCTTGATTGTTGGCCATCTTCACGACGATCCCTTTTCTCGGAAATCCTTCTCTCTCGTCTGCAAGTCCTTCCACGCCATCGAATCGCGCCAGCGGAAATCTCTCAGGCCGCTGCGCTCCGATTTGACTCCAAGTATAGCCCCGCGCTTCCCTTCCATTTCGAAGCTCGACCTTTCCCTTTGCCATCACGTGGAGGATACATTTCTGATTTCGATATCGCGTGCTTGGAAAATGACGCTGCGTTCTGTGGATCTATCCCGCTCTAGATCGTTTACCACTGTTGGATTGTCGAGCTTGGTTTCGAATTGTGCTGGACTGGTTGAGATTGACTTGTCGAACGGGGTGGCTCTGACGGACTCCGCGTTGAAGGTCATAGCAGAGGCCAAAAACTTGGAGAAATTGTGGTTGGCGAGGTGCAAATCGATTACGGATATGGGGATTGGTTGTGTGGCTGTTGGATGTAGAAAGTTGAAACACTTGTGCTTGAATTGGTGCCTTCGTATTACTGATTTGGGGGTTGATTTGATTGGAAGGAAGTGCCATGAGCTAAGGAGTTTGGACCTCTCTTTCTTACCGATCACTGAGAAATGTCTTTCGACTATTCTTCAACTGCAACAACTTGAGGAGCTAGTCCTCGAGGGTTGCCATGGAATCGATGATGGACTTGCAGTCCTAGAACGTCACTGCAAGAGCAACTCATTGAAGTTGCTTAATCTGTCTCACTGTAAAAGTATAAGTCATATTGGTTTGTCTTCTTTGATAAATGGCGCTCAAGACCTTCAACAGCTAATCTTATCATATGGCTCTTCAGTCACAACAGACGTGGCAAAATGTCTGCATAGTTTTTCGGGTTTGCAATCGATTAAATTAGATTGTTGTTCACTCACCTCTTCTGGGGTTAGAGCCATTGCAAATTGGCGTGCATCACTCAAGGAGCTGAGCTTAAGCAAGTGCTCGGGCGTGATGGACGAGTGCCTTTCCATCCTTGTGCAAAAACACAAGCAGTTGAGAAAATTAGACATCACGTGCTGCCGTAAGATAACTTTTGCATCCATTAACAGCATTACAAGTTCGTGTTCCTCCCTCGTTTCTCTTAAGATGGAATCCTGTAGCCTTGTTCCTAGAGAGGCTTATGTCTTGATAGGAGAACGCTGTCCATTCTTGGAGGAACTTGATGTCACTGACAATGAGATTGACAATGAAGGTTTGAAGTCAGTCTCAAAATGTTCAAGGCTTAGTGTTCTAAAGCTGGGGATTTGCTCGAATATAAATGATGATGGTCTATTGCACATTGCAAGTGGTTGTCCAAAAATCAAAGAGCTTGATTTGTACAGGTCAACCGGAATAACAGACAGAGGCATTGCAGCAATCGCTGGTGGTTGCCCTGCTCTTGAGATGATTAACATTGCTTATAACATTAAAGTTACAGATTCTTCGTTAATATCATTGTCAAAATGTTTGAGGTTAAAAGCACTTGAAATTCGAGGCTGTTGTTGCATTTCATCAGTTGGCCTCTCCGCCATAGCAATGGGATGTAAGCAACTTACTGTGCTGGATATAAAGAAATGTGTCAATATAAACTTGTCGTACTGTTCAGTTACAGATGTTGGGCTATTGTCGCTAGCAAGCATTAATTGCCTGAGGAACATGACCATCTTGCATTTGGCTGGCTTGACTCCGAATGGCCTCACTGCCGCCCTGCTGGTGTGTGGTACTCTACGAAAGGTAAAGCTGCATCTGTCATTCAAATCCAAGCTTCCTCCATCTTTCTCCAAGTACATGGAATCCCATGGATGCGTATTGTACTGGAGGGACAAAGCATTCCAGGTACTCATTTTC

Coding sequence (CDS)

ATGTTCTCCAACTTTGATTCTGCTCCATCTCTGAAAAACCTCAAGACCATTGACATGGATTGCCCATCTTCCGGCCCCTTTGATCATCTCACGGAGGAGATCGTGTTCTTGATTGTTGGCCATCTTCACGACGATCCCTTTTCTCGGAAATCCTTCTCTCTCGTCTGCAAGTCCTTCCACGCCATCGAATCGCGCCAGCGGAAATCTCTCAGGCCGCTGCGCTCCGATTTGACTCCAAGTATAGCCCCGCGCTTCCCTTCCATTTCGAAGCTCGACCTTTCCCTTTGCCATCACGTGGAGGATACATTTCTGATTTCGATATCGCGTGCTTGGAAAATGACGCTGCGTTCTGTGGATCTATCCCGCTCTAGATCGTTTACCACTGTTGGATTGTCGAGCTTGGTTTCGAATTGTGCTGGACTGGTTGAGATTGACTTGTCGAACGGGGTGGCTCTGACGGACTCCGCGTTGAAGGTCATAGCAGAGGCCAAAAACTTGGAGAAATTGTGGTTGGCGAGGTGCAAATCGATTACGGATATGGGGATTGGTTGTGTGGCTGTTGGATGTAGAAAGTTGAAACACTTGTGCTTGAATTGGTGCCTTCGTATTACTGATTTGGGGGTTGATTTGATTGGAAGGAAGTGCCATGAGCTAAGGAGTTTGGACCTCTCTTTCTTACCGATCACTGAGAAATGTCTTTCGACTATTCTTCAACTGCAACAACTTGAGGAGCTAGTCCTCGAGGGTTGCCATGGAATCGATGATGGACTTGCAGTCCTAGAACGTCACTGCAAGAGCAACTCATTGAAGTTGCTTAATCTGTCTCACTGTAAAAGTATAAGTCATATTGGTTTGTCTTCTTTGATAAATGGCGCTCAAGACCTTCAACAGCTAATCTTATCATATGGCTCTTCAGTCACAACAGACGTGGCAAAATGTCTGCATAGTTTTTCGGGTTTGCAATCGATTAAATTAGATTGTTGTTCACTCACCTCTTCTGGGGTTAGAGCCATTGCAAATTGGCGTGCATCACTCAAGGAGCTGAGCTTAAGCAAGTGCTCGGGCGTGATGGACGAGTGCCTTTCCATCCTTGTGCAAAAACACAAGCAGTTGAGAAAATTAGACATCACGTGCTGCCGTAAGATAACTTTTGCATCCATTAACAGCATTACAAGTTCGTGTTCCTCCCTCGTTTCTCTTAAGATGGAATCCTGTAGCCTTGTTCCTAGAGAGGCTTATGTCTTGATAGGAGAACGCTGTCCATTCTTGGAGGAACTTGATGTCACTGACAATGAGATTGACAATGAAGGTTTGAAGTCAGTCTCAAAATGTTCAAGGCTTAGTGTTCTAAAGCTGGGGATTTGCTCGAATATAAATGATGATGGTCTATTGCACATTGCAAGTGGTTGTCCAAAAATCAAAGAGCTTGATTTGTACAGGTCAACCGGAATAACAGACAGAGGCATTGCAGCAATCGCTGGTGGTTGCCCTGCTCTTGAGATGATTAACATTGCTTATAACATTAAAGTTACAGATTCTTCGTTAATATCATTGTCAAAATGTTTGAGGTTAAAAGCACTTGAAATTCGAGGCTGTTGTTGCATTTCATCAGTTGGCCTCTCCGCCATAGCAATGGGATGTAAGCAACTTACTGTGCTGGATATAAAGAAATGTGTCAATATAAACTTGTCGTACTGTTCAGTTACAGATGTTGGGCTATTGTCGCTAGCAAGCATTAATTGCCTGAGGAACATGACCATCTTGCATTTGGCTGGCTTGACTCCGAATGGCCTCACTGCCGCCCTGCTGGTGTGTGGTACTCTACGAAAGGTAAAGCTGCATCTGTCATTCAAATCCAAGCTTCCTCCATCTTTCTCCAAGTACATGGAATCCCATGGATGCGTATTGTACTGGAGGGACAAAGCATTCCAGGTACTCATTTTC

Protein sequence

MFSNFDSAPSLKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKSFHAIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSVDLSRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSITDMGIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQLQQLEELVLEGCHGIDDGLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLILSYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMDECLSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIGERCPFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELDLYRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSVGLSAIAMGCKQLTVLDIKKCVNINLSYCSVTDVGLLSLASINCLRNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWRDKAFQVLIF
Homology
BLAST of MS014175 vs. NCBI nr
Match: XP_022149653.1 (F-box/LRR-repeat protein 3-like [Momordica charantia])

HSP 1 Score: 1256.5 bits (3250), Expect = 0.0e+00
Identity = 643/663 (96.98%), Postives = 644/663 (97.13%), Query Frame = 0

Query: 1   MFSNFDSAPSLKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKSFH 60
           MFSNFDSAPSLKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKSFH
Sbjct: 1   MFSNFDSAPSLKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKSFH 60

Query: 61  AIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSVDL 120
           AIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSVDL
Sbjct: 61  AIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSVDL 120

Query: 121 SRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSITDM 180
           SRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSITDM
Sbjct: 121 SRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSITDM 180

Query: 181 GIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQLQ 240
           GIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQLQ
Sbjct: 181 GIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQLQ 240

Query: 241 QLEELVLEGCHGIDDGLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLIL 300
           +LEELVLEGCHGIDDGLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLIL
Sbjct: 241 KLEELVLEGCHGIDDGLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLIL 300

Query: 301 SYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMDEC 360
           SYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMDEC
Sbjct: 301 SYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMDEC 360

Query: 361 LSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIGERC 420
           LSILVQKHKQLRKLDI CCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIGERC
Sbjct: 361 LSILVQKHKQLRKLDIMCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIGERC 420

Query: 421 PFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELDLYR 480
           PFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELDLYR
Sbjct: 421 PFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELDLYR 480

Query: 481 STGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSVGL 540
           STGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSVGL
Sbjct: 481 STGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSVGL 540

Query: 541 SAIAMGCKQLTVLDIKKCVN------------------INLSYCSVTDVGLLSLASINCL 600
           SAIAMGCKQLTVLDIKKCVN                  INLSYCSVTDVGLLSLASINCL
Sbjct: 541 SAIAMGCKQLTVLDIKKCVNVNDDGMLPLARFSHNLKQINLSYCSVTDVGLLSLASINCL 600

Query: 601 RNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWRDKA 646
           RNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWRDKA
Sbjct: 601 RNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWRDKA 660

BLAST of MS014175 vs. NCBI nr
Match: XP_038902182.1 (F-box/LRR-repeat protein 3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1090.5 bits (2819), Expect = 0.0e+00
Identity = 559/665 (84.06%), Postives = 600/665 (90.23%), Query Frame = 0

Query: 1   MFSNFDSAPSL-KNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKSF 60
           MFSNF+S PSL K LKT +M+ P SGPFDHLTEEI+F I+ HLHDDPFSRKSFSLVCKSF
Sbjct: 1   MFSNFESTPSLKKKLKTTEMNNPFSGPFDHLTEEIIFAILDHLHDDPFSRKSFSLVCKSF 60

Query: 61  HAIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSVD 120
           +A ES  RKSLRPLRSDL  +I+PR+PSISKLDLSLC HVED+FLIS+S AWK TLRS+D
Sbjct: 61  YAAESLHRKSLRPLRSDLIRTISPRYPSISKLDLSLCSHVEDSFLISVSSAWKTTLRSID 120

Query: 121 LSRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSITD 180
           LSRSRSF+ VGLS+LV+NC GLVEI+LSNGVALTDSALKV+AEAKNLEKLWL+RCKSITD
Sbjct: 121 LSRSRSFSNVGLSNLVTNCTGLVEINLSNGVALTDSALKVLAEAKNLEKLWLSRCKSITD 180

Query: 181 MGIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQL 240
           MGIGCVAVGCRKLK LCLNWCL ITDLGV LI  KC ELRSLDLSFLPITEKCL +ILQL
Sbjct: 181 MGIGCVAVGCRKLKLLCLNWCLHITDLGVGLIATKCKELRSLDLSFLPITEKCLPSILQL 240

Query: 241 QQLEELVLEGCHGIDD-GLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQL 300
           Q LEELVLE CHGIDD GL  L+R+CK NSLK+LNLS C+SISH GLSSLING++DLQ+L
Sbjct: 241 QHLEELVLEECHGIDDEGLEALKRNCKRNSLKILNLSRCQSISHSGLSSLINGSEDLQKL 300

Query: 301 ILSYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMD 360
            LSYGSS+TTD+AKCLH+ SGLQSIKLDCCSLT+SGV+AIANWRASL+ELSLSKC+GV D
Sbjct: 301 NLSYGSSITTDMAKCLHNISGLQSIKLDCCSLTTSGVKAIANWRASLQELSLSKCTGVTD 360

Query: 361 ECLSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIGE 420
           ECLSI+VQKHKQLRKLDITCCRKIT+ASIN ITSSCSSLVSLKMESCSLVP EAYVLIGE
Sbjct: 361 ECLSIIVQKHKQLRKLDITCCRKITYASINWITSSCSSLVSLKMESCSLVPSEAYVLIGE 420

Query: 421 RCPFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELDL 480
           RCP+LEELDVTDNEIDNEGLKS+SKCSRLSVLKLGIC NINDDGLLHIASGCPKIKELDL
Sbjct: 421 RCPYLEELDVTDNEIDNEGLKSISKCSRLSVLKLGICVNINDDGLLHIASGCPKIKELDL 480

Query: 481 YRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSV 540
           YR TGITDRGIAA AGGCPALEMINIAYN K+TDSSLISLSKC RLKALEIRGCCCISSV
Sbjct: 481 YRLTGITDRGIAATAGGCPALEMINIAYNDKITDSSLISLSKCSRLKALEIRGCCCISSV 540

Query: 541 GLSAIAMGCKQLTVLDIKKCVN------------------INLSYCSVTDVGLLSLASIN 600
           GLSAIAMGCKQLTVLDIKKCVN                  INLSYCSVTDVGLLSLASIN
Sbjct: 541 GLSAIAMGCKQLTVLDIKKCVNVNDDGMLPLAQFSHNLKQINLSYCSVTDVGLLSLASIN 600

Query: 601 CLRNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWRD 646
           CLRN+TILHLAGLTP+GLTAALLVCG LRKVKLHLSFKS LPPSF KYME+ GCVLYWRD
Sbjct: 601 CLRNVTILHLAGLTPDGLTAALLVCGGLRKVKLHLSFKSLLPPSFRKYMETRGCVLYWRD 660

BLAST of MS014175 vs. NCBI nr
Match: XP_038902183.1 (F-box/LRR-repeat protein 3-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 558/664 (84.04%), Postives = 599/664 (90.21%), Query Frame = 0

Query: 1   MFSNFDSAPSL-KNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKSF 60
           MFSNF+S PSL K LKT +M+ P SGPFDHLTEEI+F I+ HLHDDPFSRKSFSLVCKSF
Sbjct: 1   MFSNFESTPSLKKKLKTTEMNNPFSGPFDHLTEEIIFAILDHLHDDPFSRKSFSLVCKSF 60

Query: 61  HAIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSVD 120
           +A ES  RKSLRPLRSDL  +I+PR+PSISKLDLSLC HVED+FLIS+S AWK TLRS+D
Sbjct: 61  YAAESLHRKSLRPLRSDLIRTISPRYPSISKLDLSLCSHVEDSFLISVSSAWKTTLRSID 120

Query: 121 LSRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSITD 180
           LSRSRSF+ VGLS+LV+NC GLVEI+LSNGVALTDSALKV+AEAKNLEKLWL+RCKSITD
Sbjct: 121 LSRSRSFSNVGLSNLVTNCTGLVEINLSNGVALTDSALKVLAEAKNLEKLWLSRCKSITD 180

Query: 181 MGIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQL 240
           MGIGCVAVGCRKLK LCLNWCL ITDLGV LI  KC ELRSLDLSFLPITEKCL +ILQL
Sbjct: 181 MGIGCVAVGCRKLKLLCLNWCLHITDLGVGLIATKCKELRSLDLSFLPITEKCLPSILQL 240

Query: 241 QQLEELVLEGCHGIDD-GLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQL 300
           Q LEELVLE CHGIDD GL  L+R+CK NSLK+LNLS C+SISH GLSSLING++DLQ+L
Sbjct: 241 QHLEELVLEECHGIDDEGLEALKRNCKRNSLKILNLSRCQSISHSGLSSLINGSEDLQKL 300

Query: 301 ILSYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMD 360
            LSYGSS+TTD+AKCLH+ SGLQSIKLDCCSLT+SGV+AIANWRASL+ELSLSKC+GV D
Sbjct: 301 NLSYGSSITTDMAKCLHNISGLQSIKLDCCSLTTSGVKAIANWRASLQELSLSKCTGVTD 360

Query: 361 ECLSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIGE 420
           ECLSI+VQKHKQLRKLDITCCRKIT+ASIN ITSSCSSLVSLKMESCSLVP EAYVLIGE
Sbjct: 361 ECLSIIVQKHKQLRKLDITCCRKITYASINWITSSCSSLVSLKMESCSLVPSEAYVLIGE 420

Query: 421 RCPFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELDL 480
           RCP+LEELDVTDNEIDNEGLKS+SKCSRLSVLKLGIC NINDDGLLHIASGCPKIKELDL
Sbjct: 421 RCPYLEELDVTDNEIDNEGLKSISKCSRLSVLKLGICVNINDDGLLHIASGCPKIKELDL 480

Query: 481 YRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSV 540
           YR TGITDRGIAA AGGCPALEMINIAYN K+TDSSLISLSKC RLKALEIRGCCCISSV
Sbjct: 481 YRLTGITDRGIAATAGGCPALEMINIAYNDKITDSSLISLSKCSRLKALEIRGCCCISSV 540

Query: 541 GLSAIAMGCKQLTVLDIKKCVN------------------INLSYCSVTDVGLLSLASIN 600
           GLSAIAMGCKQLTVLDIKKCVN                  INLSYCSVTDVGLLSLASIN
Sbjct: 541 GLSAIAMGCKQLTVLDIKKCVNVNDDGMLPLAQFSHNLKQINLSYCSVTDVGLLSLASIN 600

Query: 601 CLRNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWRD 645
           CLRN+TILHLAGLTP+GLTAALLVCG LRKVKLHLSFKS LPPSF KYME+ GCVLYWRD
Sbjct: 601 CLRNVTILHLAGLTPDGLTAALLVCGGLRKVKLHLSFKSLLPPSFRKYMETRGCVLYWRD 660

BLAST of MS014175 vs. NCBI nr
Match: XP_022986786.1 (F-box/LRR-repeat protein 3-like isoform X1 [Cucurbita maxima] >XP_022986788.1 F-box/LRR-repeat protein 3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 555/666 (83.33%), Postives = 595/666 (89.34%), Query Frame = 0

Query: 1   MFSNFDSAPS--LKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKS 60
           MFS FDS PS   K LKTIDMD   S PFDHLTEEI+F+I+ HLHDDPFSRKSFSLVCKS
Sbjct: 1   MFSKFDSGPSPLKKKLKTIDMDDAFSRPFDHLTEEIIFVILDHLHDDPFSRKSFSLVCKS 60

Query: 61  FHAIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSV 120
           F++ ES+ RKSLRPLRSDL  SIA R+PSISKLDLSLC HVED+FL +IS AWKMTL S+
Sbjct: 61  FYSTESQHRKSLRPLRSDLIRSIARRYPSISKLDLSLCLHVEDSFLNAISCAWKMTLFSI 120

Query: 121 DLSRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSIT 180
           DLSRSRSF+  GLS+LV+NCAGLVEI+LSNGVALTDSALKVIAEAKNLEKLWLARC+SIT
Sbjct: 121 DLSRSRSFSNAGLSNLVTNCAGLVEINLSNGVALTDSALKVIAEAKNLEKLWLARCRSIT 180

Query: 181 DMGIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQ 240
           DMGIGCVAVGCRKLK LCL WC+ ITD+GV LI  KC ELRSLDLSFLPITEKCL  ILQ
Sbjct: 181 DMGIGCVAVGCRKLKVLCLKWCVHITDMGVGLIATKCKELRSLDLSFLPITEKCLQPILQ 240

Query: 241 LQQLEELVLEGCHGIDD-GLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQ 300
           LQ LEELVLE CHGIDD GLA LER+CK NSLKLLNL+ C+SI H GLSSLING++DLQQ
Sbjct: 241 LQHLEELVLEECHGIDDEGLAALERYCKRNSLKLLNLTRCQSIGHSGLSSLINGSEDLQQ 300

Query: 301 LILSYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVM 360
           LILSYGSS+TTD+AKCLH+FSGLQS+KLDCCSLT+SG+RAIANWRASLKELSLSKC+GV 
Sbjct: 301 LILSYGSSITTDMAKCLHNFSGLQSLKLDCCSLTTSGLRAIANWRASLKELSLSKCAGVT 360

Query: 361 DECLSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIG 420
           DEC+S LVQKH QLRKLDITCCRKIT+ASINSITSSCSSLVSLKMESCSLVPREAYVLIG
Sbjct: 361 DECVSTLVQKHDQLRKLDITCCRKITYASINSITSSCSSLVSLKMESCSLVPREAYVLIG 420

Query: 421 ERCPFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELD 480
           + CP+LEELDVTDNEIDNEGLKSVSKCSRLSVLKLGIC NINDDGL HIA+GCPKIKELD
Sbjct: 421 KHCPYLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICLNINDDGLSHIANGCPKIKELD 480

Query: 481 LYRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISS 540
           LYRSTGITDRGIAA AGGCPALEMINIAYN K+TDSSLISLSKCLRLKALEIRGCC IS 
Sbjct: 481 LYRSTGITDRGIAATAGGCPALEMINIAYNNKITDSSLISLSKCLRLKALEIRGCCRISP 540

Query: 541 VGLSAIAMGCKQLTVLDIKKCVN------------------INLSYCSVTDVGLLSLASI 600
           +GL+AIAMGCKQLTVLDIKKCVN                  INLSYCSVTDVGLLSLASI
Sbjct: 541 IGLAAIAMGCKQLTVLDIKKCVNVNDDGMLPLAQFSHNLKQINLSYCSVTDVGLLSLASI 600

Query: 601 NCLRNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWR 646
           NCLRNMTILHLAGLTP+GLTAALLVCG LRKVKLHLSFKS LPPSF KY+E+ GCVLYWR
Sbjct: 601 NCLRNMTILHLAGLTPDGLTAALLVCGGLRKVKLHLSFKSLLPPSFRKYLETRGCVLYWR 660

BLAST of MS014175 vs. NCBI nr
Match: XP_022986789.1 (F-box/LRR-repeat protein 3-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1074.7 bits (2778), Expect = 5.0e-310
Identity = 554/665 (83.31%), Postives = 594/665 (89.32%), Query Frame = 0

Query: 1   MFSNFDSAPS--LKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKS 60
           MFS FDS PS   K LKTIDMD   S PFDHLTEEI+F+I+ HLHDDPFSRKSFSLVCKS
Sbjct: 1   MFSKFDSGPSPLKKKLKTIDMDDAFSRPFDHLTEEIIFVILDHLHDDPFSRKSFSLVCKS 60

Query: 61  FHAIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSV 120
           F++ ES+ RKSLRPLRSDL  SIA R+PSISKLDLSLC HVED+FL +IS AWKMTL S+
Sbjct: 61  FYSTESQHRKSLRPLRSDLIRSIARRYPSISKLDLSLCLHVEDSFLNAISCAWKMTLFSI 120

Query: 121 DLSRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSIT 180
           DLSRSRSF+  GLS+LV+NCAGLVEI+LSNGVALTDSALKVIAEAKNLEKLWLARC+SIT
Sbjct: 121 DLSRSRSFSNAGLSNLVTNCAGLVEINLSNGVALTDSALKVIAEAKNLEKLWLARCRSIT 180

Query: 181 DMGIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQ 240
           DMGIGCVAVGCRKLK LCL WC+ ITD+GV LI  KC ELRSLDLSFLPITEKCL  ILQ
Sbjct: 181 DMGIGCVAVGCRKLKVLCLKWCVHITDMGVGLIATKCKELRSLDLSFLPITEKCLQPILQ 240

Query: 241 LQQLEELVLEGCHGIDD-GLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQ 300
           LQ LEELVLE CHGIDD GLA LER+CK NSLKLLNL+ C+SI H GLSSLING++DLQQ
Sbjct: 241 LQHLEELVLEECHGIDDEGLAALERYCKRNSLKLLNLTRCQSIGHSGLSSLINGSEDLQQ 300

Query: 301 LILSYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVM 360
           LILSYGSS+TTD+AKCLH+FSGLQS+KLDCCSLT+SG+RAIANWRASLKELSLSKC+GV 
Sbjct: 301 LILSYGSSITTDMAKCLHNFSGLQSLKLDCCSLTTSGLRAIANWRASLKELSLSKCAGVT 360

Query: 361 DECLSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIG 420
           DEC+S LVQKH QLRKLDITCCRKIT+ASINSITSSCSSLVSLKMESCSLVPREAYVLIG
Sbjct: 361 DECVSTLVQKHDQLRKLDITCCRKITYASINSITSSCSSLVSLKMESCSLVPREAYVLIG 420

Query: 421 ERCPFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELD 480
           + CP+LEELDVTDNEIDNEGLKSVSKCSRLSVLKLGIC NINDDGL HIA+GCPKIKELD
Sbjct: 421 KHCPYLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICLNINDDGLSHIANGCPKIKELD 480

Query: 481 LYRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISS 540
           LYRSTGITDRGIAA AGGCPALEMINIAYN K+TDSSLISLSKCLRLKALEIRGCC IS 
Sbjct: 481 LYRSTGITDRGIAATAGGCPALEMINIAYNNKITDSSLISLSKCLRLKALEIRGCCRISP 540

Query: 541 VGLSAIAMGCKQLTVLDIKKCVN------------------INLSYCSVTDVGLLSLASI 600
           +GL+AIAMGCKQLTVLDIKKCVN                  INLSYCSVTDVGLLSLASI
Sbjct: 541 IGLAAIAMGCKQLTVLDIKKCVNVNDDGMLPLAQFSHNLKQINLSYCSVTDVGLLSLASI 600

Query: 601 NCLRNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWR 645
           NCLRNMTILHLAGLTP+GLTAALLVCG LRKVKLHLSFKS LPPSF KY+E+ GCVLYWR
Sbjct: 601 NCLRNMTILHLAGLTPDGLTAALLVCGGLRKVKLHLSFKSLLPPSFRKYLETRGCVLYWR 660

BLAST of MS014175 vs. ExPASy Swiss-Prot
Match: Q8RWU5 (F-box/LRR-repeat protein 3 OS=Arabidopsis thaliana OX=3702 GN=FBL3 PE=2 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 5.9e-179
Identity = 346/639 (54.15%), Postives = 440/639 (68.86%), Query Frame = 0

Query: 26  PFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKSFHAIESRQRKSLRPLRSDLTPSIAPRF 85
           PFD L+EE+VF+I+  +  +P   KSFSL CKSF+ +ES+ R SL+PLRSD  P I  R+
Sbjct: 12  PFDLLSEELVFIILDLISPNPSDLKSFSLTCKSFYQLESKHRGSLKPLRSDYLPRILTRY 71

Query: 86  PSISKLDLSLCHHVEDTFLISISRAWKMTLRSVDLSRSRSFTTVGLSSLVSNCAGLVEID 145
            + + LDL+ C  V D  L  +      TLRS+DLSRS SF+  GL  L   C  LVEID
Sbjct: 72  RNTTDLDLTFCPRVTDYALSVVGCLSGPTLRSLDLSRSGSFSAAGLLRLALKCVNLVEID 131

Query: 146 LSNGVALTDSALKVIAEAKNLEKLWLARCKSITDMGIGCVAVGCRKLKHLCLNWCLRITD 205
           LSN   + D+   V+AEA++LE+L L RCK +TDMGIGC+AVGC+KL  + L WC+ + D
Sbjct: 132 LSNATEMRDADAAVVAEARSLERLKLGRCKMLTDMGIGCIAVGCKKLNTVSLKWCVGVGD 191

Query: 206 LGVDLIGRKCHELRSLDLSFLPITEKCLSTILQLQQLEELVLEGCHGI-DDGLAVLERHC 265
           LGV L+  KC ++R+LDLS+LPIT KCL  IL+LQ LEEL+LEGC G+ DD L  L   C
Sbjct: 192 LGVGLLAVKCKDIRTLDLSYLPITGKCLHDILKLQHLEELLLEGCFGVDDDSLKSLRHDC 251

Query: 266 KSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLILSYGSSV-TTDVAKCLHSFSGLQSI 325
           K  SLK L+ S C++++H GL+SL++GA  LQ+L LS+ SSV + D A  L   S LQSI
Sbjct: 252 K--SLKKLDASSCQNLTHRGLTSLLSGAGYLQRLDLSHCSSVISLDFASSLKKVSALQSI 311

Query: 326 KLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMDECLSILVQKHKQLRKLDITCCRKIT 385
           +LD CS+T  G++AI     SLKE+SLSKC  V DE LS LV K K LRKLDITCCRK++
Sbjct: 312 RLDGCSVTPDGLKAIGTLCNSLKEVSLSKCVSVTDEGLSSLVMKLKDLRKLDITCCRKLS 371

Query: 386 FASINSITSSCSSLVSLKMESCSLVPREAYVLIGERCPFLEELDVTDNEIDNEGLKSVSK 445
             SI  I +SC  LVSLKMESCSLV REA+ LIG++C  LEELD+TDNEID+EGLKS+S 
Sbjct: 372 RVSITQIANSCPLLVSLKMESCSLVSREAFWLIGQKCRLLEELDLTDNEIDDEGLKSISS 431

Query: 446 CSRLSVLKLGICSNINDDGLLHIASGCPKIKELDLYRSTGITDRGIAAIAGGCPALEMIN 505
           C  LS LKLGIC NI D GL +I  GC  ++ELDLYRS GITD GI+ IA GC  LE IN
Sbjct: 432 CLSLSSLKLGICLNITDKGLSYIGMGCSNLRELDLYRSVGITDVGISTIAQGCIHLETIN 491

Query: 506 IAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSVGLSAIAMGCKQLTVLDIKKCVN--- 565
           I+Y   +TD SL+SLSKC  L+  E RGC  I+S GL+AIA+ CK+L  +D+KKC +   
Sbjct: 492 ISYCQDITDKSLVSLSKCSLLQTFESRGCPNITSQGLAAIAVRCKRLAKVDLKKCPSIND 551

Query: 566 ---------------INLSYCSVTDVGLLSLASINCLRNMTILHLAGLTPNGLTAALLVC 625
                          IN+S  +VT+VGLLSLA+I CL+N+ +++ +GL P+G+ AALL C
Sbjct: 552 AGLLALAHFSQNLKQINVSDTAVTEVGLLSLANIGCLQNIAVVNSSGLRPSGVAAALLGC 611

Query: 626 GTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWRDKAFQ 645
           G LRK KLH S +S LP S   ++E+ GC   W+D   Q
Sbjct: 612 GGLRKAKLHASLRSLLPLSLIHHLEARGCAFLWKDNTLQ 648

BLAST of MS014175 vs. ExPASy Swiss-Prot
Match: Q9C5D2 (F-box/LRR-repeat protein 4 OS=Arabidopsis thaliana OX=3702 GN=FBL4 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 1.1e-44
Identity = 169/610 (27.70%), Postives = 282/610 (46.23%), Query Frame = 0

Query: 30  LTEEIVFLIVGHLHDDPFSRKSFSLVCKSFHAIESRQRKSLRPLRS----DLTPSIAPRF 89
           L EE++  I   L   P +R + SLVCK + ++E   R +LR   S    D    ++ RF
Sbjct: 11  LPEELILEIFRRLESKP-NRDACSLVCKRWLSLERFSRTTLRIGASFSPDDFISLLSRRF 70

Query: 90  PSISKLDLSLCHHVEDTFLISISRAWKMTLRSVDLSRSRSFTTVGLSSLVSNCAGLVEID 149
             I+ +      HV++   +S+         S    R R   +   SS            
Sbjct: 71  LYITSI------HVDERISVSLPSL------SPSPKRKRGRDSSSPSS------------ 130

Query: 150 LSNGVALTDSALKVIAEAKNLEKLWLARCKSITDMGIGCVAVGCRKLKHLCLNWCLRITD 209
            S    LTD   K  + A+N+E        S+TD G+  +A G  ++++L L WC  ++ 
Sbjct: 131 -SKRKKLTD---KTHSGAENVES------SSLTDTGLTALANGFPRIENLSLIWCPNVSS 190

Query: 210 LGVDLIGRKCHELRSLDLSFLPITEKCLSTILQL-QQLEELVLEGCHGIDD-GLAVLERH 269
           +G+  + +KC  L+SLDL    + ++ L+ + +  +QLEEL L  C G+ D G+  L   
Sbjct: 191 VGLCSLAQKCTSLKSLDLQGCYVGDQGLAAVGKFCKQLEELNLRFCEGLTDVGVIDLVVG 250

Query: 270 CKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLILSYGSSVTTDVAKCLHSFSGLQSI 329
           C S SLK + ++    I+ + L ++ +  + L+ L L         +         L+++
Sbjct: 251 C-SKSLKSIGVAASAKITDLSLEAVGSHCKLLEVLYLDSEYIHDKGLIAVAQGCHRLKNL 310

Query: 330 KLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMDECLSILVQKHKQLRKLDITCCRKIT 389
           KL C S+T     A+     SL+ L+L       D+ +  + +  K+L+ L ++ C  ++
Sbjct: 311 KLQCVSVTDVAFAAVGELCTSLERLALYSFQHFTDKGMRAIGKGSKKLKDLTLSDCYFVS 370

Query: 390 FASINSITSSCSSLVSLKMESCSLVPREAYVLIGERCPFLEELDVT-DNEIDNEGLKSVS 449
              + +I   C  L  +++  C  +       IG+ CP L+EL +     I N  L+ + 
Sbjct: 371 CKGLEAIAHGCKELERVEINGCHNIGTRGIEAIGKSCPRLKELALLYCQRIGNSALQEIG 430

Query: 450 K-CSRLSVLKLGICSNINDDGLLHIASGCPKIKELDLYRSTGITDRGIAAIAGGCPALEM 509
           K C  L +L L  CS I D  +  IA GC  +K+L + R   I ++GI +I   C +L  
Sbjct: 431 KGCKSLEILHLVDCSGIGDIAMCSIAKGCRNLKKLHIRRCYEIGNKGIISIGKHCKSLTE 490

Query: 510 INIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSVGLSAIAMGCKQLTVLDIKKCVNI 569
           +++ +  KV + +LI++ K   L+ L + GC  IS  G++AIA GC QLT LDI    NI
Sbjct: 491 LSLRFCDKVGNKALIAIGKGCSLQQLNVSGCNQISDAGITAIARGCPQLTHLDISVLQNI 550

Query: 570 N------------------LSYC-SVTDVGLLSLA-SINCLRNMTILHLAGLTPNGLTAA 612
                              LS+C  +TD GL  L      L    +++  G+T  G+   
Sbjct: 551 GDMPLAELGEGCPMLKDLVLSHCHHITDNGLNHLVQKCKLLETCHMVYCPGITSAGVATV 584

BLAST of MS014175 vs. ExPASy Swiss-Prot
Match: Q8NEE6 (Dynein regulatory complex subunit 6 OS=Homo sapiens OX=9606 GN=FBXL13 PE=2 SV=3)

HSP 1 Score: 133.3 bits (334), Expect = 1.0e-29
Identity = 113/456 (24.78%), Postives = 217/456 (47.59%), Query Frame = 0

Query: 115 LRSVDLSRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVI-AEAKNLEKLWLAR 174
           L+ +++S   +FT   +  +   C G++ ++LSN   +T+  ++++     NL+ L LA 
Sbjct: 247 LQELNVSDCPTFTDESMRHISEGCPGVLCLNLSN-TTITNRTMRLLPRHFHNLQNLSLAY 306

Query: 175 CKSITDMGIGCVAV--GCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLP-ITE 234
           C+  TD G+  + +  GC KL +L L+ C +I+  G   I   C  +  L ++ +P +T+
Sbjct: 307 CRRFTDKGLQYLNLGNGCHKLIYLDLSGCTQISVQGFRYIANSCTGIMHLTINDMPTLTD 366

Query: 235 KCLSTILQ-LQQLEELVLEGCHGIDDGLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLI 294
            C+  +++   ++  LV  G   I D        CK   L+ +     K ++      + 
Sbjct: 367 NCVKALVEKCSRITSLVFTGAPHISDCTFRALSACK---LRKIRFEGNKRVTDASFKFID 426

Query: 295 NGAQDLQQLILSYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELS 354
               +L  + ++                        DC  +T S +R+++  +  L  L+
Sbjct: 427 KNYPNLSHIYMA------------------------DCKGITDSSLRSLSPLK-QLTVLN 486

Query: 355 LSKCSGVMDECLSILVQ--KHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMESCSL 414
           L+ C  + D  L   +      ++R+L+++ C +++ AS+  ++  C +L  L + +C  
Sbjct: 487 LANCVRIGDMGLKQFLDGPASMRIRELNLSNCVRLSDASVMKLSERCPNLNYLSLRNCEH 546

Query: 415 VPREAYVLIGERCPFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIA 474
           +  +    I      L  +D++  +I NEGL  +S+  +L  L +  C  I DDG+    
Sbjct: 547 LTAQGIGYI-VNIFSLVSIDLSGTDISNEGLNVLSRHKKLKELSVSECYRITDDGIQAFC 606

Query: 475 SGCPKIKELDLYRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLS-KCLRLKA 534
                ++ LD+   + ++D  I A+A  C  L  ++IA   K+TDS++  LS KC  L  
Sbjct: 607 KSSLILEHLDVSYCSQLSDMIIKALAIYCINLTSLSIAGCPKITDSAMEMLSAKCHYLHI 666

Query: 535 LEIRGCCCISSVGLSAIAMGCKQLTVLDIKKCVNIN 563
           L+I GC  ++   L  + +GCKQL +L ++ C NI+
Sbjct: 667 LDISGCVLLTDQILEDLQIGCKQLRILKMQYCTNIS 672

BLAST of MS014175 vs. ExPASy Swiss-Prot
Match: Q58DG6 (F-box/LRR-repeat protein 20 OS=Bos taurus OX=9913 GN=FBXL20 PE=2 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 6.1e-27
Identity = 80/259 (30.89%), Postives = 141/259 (54.44%), Query Frame = 0

Query: 345 LKELSLSKCSGVMDECLSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMES 404
           L++LSL  C GV D  L    Q  + +  L++  C K T A+  S++  CS L  L + S
Sbjct: 93  LRKLSLRGCLGVGDNALRTFAQNCRNIEVLNLNGCTKTTDATCTSLSKFCSKLRHLDLAS 152

Query: 405 CSLVPREAYVLIGERCPFLEELDVT-DNEIDNEGLKS-VSKCSRLSVLKLGICSNINDDG 464
           C+ +   +   + E CP LE+L+++  +++  +G+++ V  C  L  L L  C+ + D+ 
Sbjct: 153 CTSITNMSLKALSEGCPLLEQLNISWCDQVTKDGIQALVRGCGGLKALFLKGCTQLEDEA 212

Query: 465 LLHIASGCPKIKELDLYRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSK-C 524
           L +I + CP++  L+L     ITD G+  I  GC  L+ +  +    +TD+ L +L + C
Sbjct: 213 LKYIGAHCPELVTLNLQTCLQITDEGLITICRGCHKLQSLCASGCSNITDAILNALGQNC 272

Query: 525 LRLKALEIRGCCCISSVGLSAIAMGCKQLTVLDIKKCVNINLSYCSVTDVGLLSLASINC 584
            RL+ LE+  C  ++ VG + +A  C +L  +D+++CV I       TD  L+ L SI+C
Sbjct: 273 PRLRILEVARCSQLTDVGFTTLARNCHELEKMDLEECVQI-------TDSTLIQL-SIHC 332

Query: 585 --LRNMTILHLAGLTPNGL 599
             L+ +++ H   +T +G+
Sbjct: 333 PRLQVLSLSHCELITDDGI 343

BLAST of MS014175 vs. ExPASy Swiss-Prot
Match: Q96IG2 (F-box/LRR-repeat protein 20 OS=Homo sapiens OX=9606 GN=FBXL20 PE=1 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 6.1e-27
Identity = 80/259 (30.89%), Postives = 141/259 (54.44%), Query Frame = 0

Query: 345 LKELSLSKCSGVMDECLSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMES 404
           L++LSL  C GV D  L    Q  + +  L++  C K T A+  S++  CS L  L + S
Sbjct: 93  LRKLSLRGCLGVGDNALRTFAQNCRNIEVLNLNGCTKTTDATCTSLSKFCSKLRHLDLAS 152

Query: 405 CSLVPREAYVLIGERCPFLEELDVT-DNEIDNEGLKS-VSKCSRLSVLKLGICSNINDDG 464
           C+ +   +   + E CP LE+L+++  +++  +G+++ V  C  L  L L  C+ + D+ 
Sbjct: 153 CTSITNMSLKALSEGCPLLEQLNISWCDQVTKDGIQALVRGCGGLKALFLKGCTQLEDEA 212

Query: 465 LLHIASGCPKIKELDLYRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSK-C 524
           L +I + CP++  L+L     ITD G+  I  GC  L+ +  +    +TD+ L +L + C
Sbjct: 213 LKYIGAHCPELVTLNLQTCLQITDEGLITICRGCHKLQSLCASGCSNITDAILNALGQNC 272

Query: 525 LRLKALEIRGCCCISSVGLSAIAMGCKQLTVLDIKKCVNINLSYCSVTDVGLLSLASINC 584
            RL+ LE+  C  ++ VG + +A  C +L  +D+++CV I       TD  L+ L SI+C
Sbjct: 273 PRLRILEVARCSQLTDVGFTTLARNCHELEKMDLEECVQI-------TDSTLIQL-SIHC 332

Query: 585 --LRNMTILHLAGLTPNGL 599
             L+ +++ H   +T +G+
Sbjct: 333 PRLQVLSLSHCELITDDGI 343

BLAST of MS014175 vs. ExPASy TrEMBL
Match: A0A6J1D7C8 (F-box/LRR-repeat protein 3-like OS=Momordica charantia OX=3673 GN=LOC111018032 PE=4 SV=1)

HSP 1 Score: 1256.5 bits (3250), Expect = 0.0e+00
Identity = 643/663 (96.98%), Postives = 644/663 (97.13%), Query Frame = 0

Query: 1   MFSNFDSAPSLKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKSFH 60
           MFSNFDSAPSLKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKSFH
Sbjct: 1   MFSNFDSAPSLKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKSFH 60

Query: 61  AIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSVDL 120
           AIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSVDL
Sbjct: 61  AIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSVDL 120

Query: 121 SRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSITDM 180
           SRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSITDM
Sbjct: 121 SRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSITDM 180

Query: 181 GIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQLQ 240
           GIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQLQ
Sbjct: 181 GIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQLQ 240

Query: 241 QLEELVLEGCHGIDDGLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLIL 300
           +LEELVLEGCHGIDDGLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLIL
Sbjct: 241 KLEELVLEGCHGIDDGLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLIL 300

Query: 301 SYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMDEC 360
           SYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMDEC
Sbjct: 301 SYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMDEC 360

Query: 361 LSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIGERC 420
           LSILVQKHKQLRKLDI CCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIGERC
Sbjct: 361 LSILVQKHKQLRKLDIMCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIGERC 420

Query: 421 PFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELDLYR 480
           PFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELDLYR
Sbjct: 421 PFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELDLYR 480

Query: 481 STGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSVGL 540
           STGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSVGL
Sbjct: 481 STGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSVGL 540

Query: 541 SAIAMGCKQLTVLDIKKCVN------------------INLSYCSVTDVGLLSLASINCL 600
           SAIAMGCKQLTVLDIKKCVN                  INLSYCSVTDVGLLSLASINCL
Sbjct: 541 SAIAMGCKQLTVLDIKKCVNVNDDGMLPLARFSHNLKQINLSYCSVTDVGLLSLASINCL 600

Query: 601 RNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWRDKA 646
           RNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWRDKA
Sbjct: 601 RNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWRDKA 660

BLAST of MS014175 vs. ExPASy TrEMBL
Match: A0A6J1JHJ8 (F-box/LRR-repeat protein 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484436 PE=4 SV=1)

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 555/666 (83.33%), Postives = 595/666 (89.34%), Query Frame = 0

Query: 1   MFSNFDSAPS--LKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKS 60
           MFS FDS PS   K LKTIDMD   S PFDHLTEEI+F+I+ HLHDDPFSRKSFSLVCKS
Sbjct: 1   MFSKFDSGPSPLKKKLKTIDMDDAFSRPFDHLTEEIIFVILDHLHDDPFSRKSFSLVCKS 60

Query: 61  FHAIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSV 120
           F++ ES+ RKSLRPLRSDL  SIA R+PSISKLDLSLC HVED+FL +IS AWKMTL S+
Sbjct: 61  FYSTESQHRKSLRPLRSDLIRSIARRYPSISKLDLSLCLHVEDSFLNAISCAWKMTLFSI 120

Query: 121 DLSRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSIT 180
           DLSRSRSF+  GLS+LV+NCAGLVEI+LSNGVALTDSALKVIAEAKNLEKLWLARC+SIT
Sbjct: 121 DLSRSRSFSNAGLSNLVTNCAGLVEINLSNGVALTDSALKVIAEAKNLEKLWLARCRSIT 180

Query: 181 DMGIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQ 240
           DMGIGCVAVGCRKLK LCL WC+ ITD+GV LI  KC ELRSLDLSFLPITEKCL  ILQ
Sbjct: 181 DMGIGCVAVGCRKLKVLCLKWCVHITDMGVGLIATKCKELRSLDLSFLPITEKCLQPILQ 240

Query: 241 LQQLEELVLEGCHGIDD-GLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQ 300
           LQ LEELVLE CHGIDD GLA LER+CK NSLKLLNL+ C+SI H GLSSLING++DLQQ
Sbjct: 241 LQHLEELVLEECHGIDDEGLAALERYCKRNSLKLLNLTRCQSIGHSGLSSLINGSEDLQQ 300

Query: 301 LILSYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVM 360
           LILSYGSS+TTD+AKCLH+FSGLQS+KLDCCSLT+SG+RAIANWRASLKELSLSKC+GV 
Sbjct: 301 LILSYGSSITTDMAKCLHNFSGLQSLKLDCCSLTTSGLRAIANWRASLKELSLSKCAGVT 360

Query: 361 DECLSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIG 420
           DEC+S LVQKH QLRKLDITCCRKIT+ASINSITSSCSSLVSLKMESCSLVPREAYVLIG
Sbjct: 361 DECVSTLVQKHDQLRKLDITCCRKITYASINSITSSCSSLVSLKMESCSLVPREAYVLIG 420

Query: 421 ERCPFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELD 480
           + CP+LEELDVTDNEIDNEGLKSVSKCSRLSVLKLGIC NINDDGL HIA+GCPKIKELD
Sbjct: 421 KHCPYLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICLNINDDGLSHIANGCPKIKELD 480

Query: 481 LYRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISS 540
           LYRSTGITDRGIAA AGGCPALEMINIAYN K+TDSSLISLSKCLRLKALEIRGCC IS 
Sbjct: 481 LYRSTGITDRGIAATAGGCPALEMINIAYNNKITDSSLISLSKCLRLKALEIRGCCRISP 540

Query: 541 VGLSAIAMGCKQLTVLDIKKCVN------------------INLSYCSVTDVGLLSLASI 600
           +GL+AIAMGCKQLTVLDIKKCVN                  INLSYCSVTDVGLLSLASI
Sbjct: 541 IGLAAIAMGCKQLTVLDIKKCVNVNDDGMLPLAQFSHNLKQINLSYCSVTDVGLLSLASI 600

Query: 601 NCLRNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWR 646
           NCLRNMTILHLAGLTP+GLTAALLVCG LRKVKLHLSFKS LPPSF KY+E+ GCVLYWR
Sbjct: 601 NCLRNMTILHLAGLTPDGLTAALLVCGGLRKVKLHLSFKSLLPPSFRKYLETRGCVLYWR 660

BLAST of MS014175 vs. ExPASy TrEMBL
Match: A0A6J1JH24 (F-box/LRR-repeat protein 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484436 PE=4 SV=1)

HSP 1 Score: 1074.7 bits (2778), Expect = 2.4e-310
Identity = 554/665 (83.31%), Postives = 594/665 (89.32%), Query Frame = 0

Query: 1   MFSNFDSAPS--LKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKS 60
           MFS FDS PS   K LKTIDMD   S PFDHLTEEI+F+I+ HLHDDPFSRKSFSLVCKS
Sbjct: 1   MFSKFDSGPSPLKKKLKTIDMDDAFSRPFDHLTEEIIFVILDHLHDDPFSRKSFSLVCKS 60

Query: 61  FHAIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSV 120
           F++ ES+ RKSLRPLRSDL  SIA R+PSISKLDLSLC HVED+FL +IS AWKMTL S+
Sbjct: 61  FYSTESQHRKSLRPLRSDLIRSIARRYPSISKLDLSLCLHVEDSFLNAISCAWKMTLFSI 120

Query: 121 DLSRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSIT 180
           DLSRSRSF+  GLS+LV+NCAGLVEI+LSNGVALTDSALKVIAEAKNLEKLWLARC+SIT
Sbjct: 121 DLSRSRSFSNAGLSNLVTNCAGLVEINLSNGVALTDSALKVIAEAKNLEKLWLARCRSIT 180

Query: 181 DMGIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQ 240
           DMGIGCVAVGCRKLK LCL WC+ ITD+GV LI  KC ELRSLDLSFLPITEKCL  ILQ
Sbjct: 181 DMGIGCVAVGCRKLKVLCLKWCVHITDMGVGLIATKCKELRSLDLSFLPITEKCLQPILQ 240

Query: 241 LQQLEELVLEGCHGIDD-GLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQ 300
           LQ LEELVLE CHGIDD GLA LER+CK NSLKLLNL+ C+SI H GLSSLING++DLQQ
Sbjct: 241 LQHLEELVLEECHGIDDEGLAALERYCKRNSLKLLNLTRCQSIGHSGLSSLINGSEDLQQ 300

Query: 301 LILSYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVM 360
           LILSYGSS+TTD+AKCLH+FSGLQS+KLDCCSLT+SG+RAIANWRASLKELSLSKC+GV 
Sbjct: 301 LILSYGSSITTDMAKCLHNFSGLQSLKLDCCSLTTSGLRAIANWRASLKELSLSKCAGVT 360

Query: 361 DECLSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIG 420
           DEC+S LVQKH QLRKLDITCCRKIT+ASINSITSSCSSLVSLKMESCSLVPREAYVLIG
Sbjct: 361 DECVSTLVQKHDQLRKLDITCCRKITYASINSITSSCSSLVSLKMESCSLVPREAYVLIG 420

Query: 421 ERCPFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELD 480
           + CP+LEELDVTDNEIDNEGLKSVSKCSRLSVLKLGIC NINDDGL HIA+GCPKIKELD
Sbjct: 421 KHCPYLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICLNINDDGLSHIANGCPKIKELD 480

Query: 481 LYRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISS 540
           LYRSTGITDRGIAA AGGCPALEMINIAYN K+TDSSLISLSKCLRLKALEIRGCC IS 
Sbjct: 481 LYRSTGITDRGIAATAGGCPALEMINIAYNNKITDSSLISLSKCLRLKALEIRGCCRISP 540

Query: 541 VGLSAIAMGCKQLTVLDIKKCVN------------------INLSYCSVTDVGLLSLASI 600
           +GL+AIAMGCKQLTVLDIKKCVN                  INLSYCSVTDVGLLSLASI
Sbjct: 541 IGLAAIAMGCKQLTVLDIKKCVNVNDDGMLPLAQFSHNLKQINLSYCSVTDVGLLSLASI 600

Query: 601 NCLRNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWR 645
           NCLRNMTILHLAGLTP+GLTAALLVCG LRKVKLHLSFKS LPPSF KY+E+ GCVLYWR
Sbjct: 601 NCLRNMTILHLAGLTPDGLTAALLVCGGLRKVKLHLSFKSLLPPSFRKYLETRGCVLYWR 660

BLAST of MS014175 vs. ExPASy TrEMBL
Match: A0A6J1FYI6 (F-box/LRR-repeat protein 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448713 PE=4 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 3.1e-308
Identity = 550/666 (82.58%), Postives = 591/666 (88.74%), Query Frame = 0

Query: 1   MFSNFDSAPS--LKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKS 60
           MFS  DSAPS   K +KTIDMD   S PFDHLTEEI+F+I+ HLHDDPFSRKSFSLVCKS
Sbjct: 1   MFSKVDSAPSPLKKKIKTIDMDDAFSRPFDHLTEEIIFVILDHLHDDPFSRKSFSLVCKS 60

Query: 61  FHAIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSV 120
           F++ ES  RKSLRPLRSDL  SIA R+PSISKLDLSLC HVED+FL +IS AWK TL S+
Sbjct: 61  FYSAESYHRKSLRPLRSDLIRSIARRYPSISKLDLSLCLHVEDSFLNAISCAWKTTLFSI 120

Query: 121 DLSRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSIT 180
           DLSRSRSF+  GLS+LV+NCAGLVEI+LSNGVALTDSALKVIAEAKNLEKLWLARC+SIT
Sbjct: 121 DLSRSRSFSNAGLSNLVTNCAGLVEINLSNGVALTDSALKVIAEAKNLEKLWLARCRSIT 180

Query: 181 DMGIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQ 240
           DMGIGCVAVGCRKLK LCL WC+ ITD+GV LI  KC ELRSLDLSFLPITEKCL  ILQ
Sbjct: 181 DMGIGCVAVGCRKLKVLCLKWCVHITDMGVGLIATKCKELRSLDLSFLPITEKCLQPILQ 240

Query: 241 LQQLEELVLEGCHGIDD-GLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQ 300
           LQ LEELVLE CHGIDD GLA  ER+CK NSLKLLNL+ C+SISH GLSSLING++DLQQ
Sbjct: 241 LQHLEELVLEECHGIDDEGLAAFERYCKRNSLKLLNLTRCQSISHSGLSSLINGSEDLQQ 300

Query: 301 LILSYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVM 360
           LILSYGSS+TTD+AKCLH+FSGLQS+KLDCCSLT+SGVRAIANWRASLKELSLSKC+GV 
Sbjct: 301 LILSYGSSITTDMAKCLHNFSGLQSLKLDCCSLTTSGVRAIANWRASLKELSLSKCAGVT 360

Query: 361 DECLSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIG 420
           DEC+S LVQKH QLRKLDITCCRKIT+ASINSITSSCSSLVSLKMESCSLVPREAYVLIG
Sbjct: 361 DECVSTLVQKHNQLRKLDITCCRKITYASINSITSSCSSLVSLKMESCSLVPREAYVLIG 420

Query: 421 ERCPFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELD 480
           + CP+LEELDVTDNEIDNEGLKS+SKCSRLSVLKLGIC NINDDGL HIA+GCPKIKELD
Sbjct: 421 KHCPYLEELDVTDNEIDNEGLKSISKCSRLSVLKLGICLNINDDGLSHIANGCPKIKELD 480

Query: 481 LYRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISS 540
           LYRSTGITDRGIAA A GCP LEMINIAYN K+TDSSL SLSKCLRLKALEIRGCC ISS
Sbjct: 481 LYRSTGITDRGIAATAEGCPVLEMINIAYNNKITDSSLKSLSKCLRLKALEIRGCCSISS 540

Query: 541 VGLSAIAMGCKQLTVLDIKKCVN------------------INLSYCSVTDVGLLSLASI 600
           +GL+A+AMGCKQLTVLDIKKCVN                  INLSYCSVTDVGLLSLASI
Sbjct: 541 IGLAAVAMGCKQLTVLDIKKCVNVNDDGMLPLAQFSHNLKQINLSYCSVTDVGLLSLASI 600

Query: 601 NCLRNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWR 646
           NCLRNMTILHLAGLTP+GLTAALLVCG LRKVKLHLSFKS LPPSF KY+E+ GCVLYWR
Sbjct: 601 NCLRNMTILHLAGLTPDGLTAALLVCGGLRKVKLHLSFKSLLPPSFRKYLETRGCVLYWR 660

BLAST of MS014175 vs. ExPASy TrEMBL
Match: A0A6J1FV45 (F-box/LRR-repeat protein 3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448713 PE=4 SV=1)

HSP 1 Score: 1065.4 bits (2754), Expect = 9.0e-308
Identity = 549/665 (82.56%), Postives = 590/665 (88.72%), Query Frame = 0

Query: 1   MFSNFDSAPS--LKNLKTIDMDCPSSGPFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKS 60
           MFS  DSAPS   K +KTIDMD   S PFDHLTEEI+F+I+ HLHDDPFSRKSFSLVCKS
Sbjct: 1   MFSKVDSAPSPLKKKIKTIDMDDAFSRPFDHLTEEIIFVILDHLHDDPFSRKSFSLVCKS 60

Query: 61  FHAIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSV 120
           F++ ES  RKSLRPLRSDL  SIA R+PSISKLDLSLC HVED+FL +IS AWK TL S+
Sbjct: 61  FYSAESYHRKSLRPLRSDLIRSIARRYPSISKLDLSLCLHVEDSFLNAISCAWKTTLFSI 120

Query: 121 DLSRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSIT 180
           DLSRSRSF+  GLS+LV+NCAGLVEI+LSNGVALTDSALKVIAEAKNLEKLWLARC+SIT
Sbjct: 121 DLSRSRSFSNAGLSNLVTNCAGLVEINLSNGVALTDSALKVIAEAKNLEKLWLARCRSIT 180

Query: 181 DMGIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQ 240
           DMGIGCVAVGCRKLK LCL WC+ ITD+GV LI  KC ELRSLDLSFLPITEKCL  ILQ
Sbjct: 181 DMGIGCVAVGCRKLKVLCLKWCVHITDMGVGLIATKCKELRSLDLSFLPITEKCLQPILQ 240

Query: 241 LQQLEELVLEGCHGIDD-GLAVLERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQ 300
           LQ LEELVLE CHGIDD GLA  ER+CK NSLKLLNL+ C+SISH GLSSLING++DLQQ
Sbjct: 241 LQHLEELVLEECHGIDDEGLAAFERYCKRNSLKLLNLTRCQSISHSGLSSLINGSEDLQQ 300

Query: 301 LILSYGSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIANWRASLKELSLSKCSGVM 360
           LILSYGSS+TTD+AKCLH+FSGLQS+KLDCCSLT+SGVRAIANWRASLKELSLSKC+GV 
Sbjct: 301 LILSYGSSITTDMAKCLHNFSGLQSLKLDCCSLTTSGVRAIANWRASLKELSLSKCAGVT 360

Query: 361 DECLSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVSLKMESCSLVPREAYVLIG 420
           DEC+S LVQKH QLRKLDITCCRKIT+ASINSITSSCSSLVSLKMESCSLVPREAYVLIG
Sbjct: 361 DECVSTLVQKHNQLRKLDITCCRKITYASINSITSSCSSLVSLKMESCSLVPREAYVLIG 420

Query: 421 ERCPFLEELDVTDNEIDNEGLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELD 480
           + CP+LEELDVTDNEIDNEGLKS+SKCSRLSVLKLGIC NINDDGL HIA+GCPKIKELD
Sbjct: 421 KHCPYLEELDVTDNEIDNEGLKSISKCSRLSVLKLGICLNINDDGLSHIANGCPKIKELD 480

Query: 481 LYRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISS 540
           LYRSTGITDRGIAA A GCP LEMINIAYN K+TDSSL SLSKCLRLKALEIRGCC ISS
Sbjct: 481 LYRSTGITDRGIAATAEGCPVLEMINIAYNNKITDSSLKSLSKCLRLKALEIRGCCSISS 540

Query: 541 VGLSAIAMGCKQLTVLDIKKCVN------------------INLSYCSVTDVGLLSLASI 600
           +GL+A+AMGCKQLTVLDIKKCVN                  INLSYCSVTDVGLLSLASI
Sbjct: 541 IGLAAVAMGCKQLTVLDIKKCVNVNDDGMLPLAQFSHNLKQINLSYCSVTDVGLLSLASI 600

Query: 601 NCLRNMTILHLAGLTPNGLTAALLVCGTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWR 645
           NCLRNMTILHLAGLTP+GLTAALLVCG LRKVKLHLSFKS LPPSF KY+E+ GCVLYWR
Sbjct: 601 NCLRNMTILHLAGLTPDGLTAALLVCGGLRKVKLHLSFKSLLPPSFRKYLETRGCVLYWR 660

BLAST of MS014175 vs. TAIR 10
Match: AT5G01720.1 (RNI-like superfamily protein )

HSP 1 Score: 629.0 bits (1621), Expect = 4.2e-180
Identity = 346/639 (54.15%), Postives = 440/639 (68.86%), Query Frame = 0

Query: 26  PFDHLTEEIVFLIVGHLHDDPFSRKSFSLVCKSFHAIESRQRKSLRPLRSDLTPSIAPRF 85
           PFD L+EE+VF+I+  +  +P   KSFSL CKSF+ +ES+ R SL+PLRSD  P I  R+
Sbjct: 12  PFDLLSEELVFIILDLISPNPSDLKSFSLTCKSFYQLESKHRGSLKPLRSDYLPRILTRY 71

Query: 86  PSISKLDLSLCHHVEDTFLISISRAWKMTLRSVDLSRSRSFTTVGLSSLVSNCAGLVEID 145
            + + LDL+ C  V D  L  +      TLRS+DLSRS SF+  GL  L   C  LVEID
Sbjct: 72  RNTTDLDLTFCPRVTDYALSVVGCLSGPTLRSLDLSRSGSFSAAGLLRLALKCVNLVEID 131

Query: 146 LSNGVALTDSALKVIAEAKNLEKLWLARCKSITDMGIGCVAVGCRKLKHLCLNWCLRITD 205
           LSN   + D+   V+AEA++LE+L L RCK +TDMGIGC+AVGC+KL  + L WC+ + D
Sbjct: 132 LSNATEMRDADAAVVAEARSLERLKLGRCKMLTDMGIGCIAVGCKKLNTVSLKWCVGVGD 191

Query: 206 LGVDLIGRKCHELRSLDLSFLPITEKCLSTILQLQQLEELVLEGCHGI-DDGLAVLERHC 265
           LGV L+  KC ++R+LDLS+LPIT KCL  IL+LQ LEEL+LEGC G+ DD L  L   C
Sbjct: 192 LGVGLLAVKCKDIRTLDLSYLPITGKCLHDILKLQHLEELLLEGCFGVDDDSLKSLRHDC 251

Query: 266 KSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLILSYGSSV-TTDVAKCLHSFSGLQSI 325
           K  SLK L+ S C++++H GL+SL++GA  LQ+L LS+ SSV + D A  L   S LQSI
Sbjct: 252 K--SLKKLDASSCQNLTHRGLTSLLSGAGYLQRLDLSHCSSVISLDFASSLKKVSALQSI 311

Query: 326 KLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMDECLSILVQKHKQLRKLDITCCRKIT 385
           +LD CS+T  G++AI     SLKE+SLSKC  V DE LS LV K K LRKLDITCCRK++
Sbjct: 312 RLDGCSVTPDGLKAIGTLCNSLKEVSLSKCVSVTDEGLSSLVMKLKDLRKLDITCCRKLS 371

Query: 386 FASINSITSSCSSLVSLKMESCSLVPREAYVLIGERCPFLEELDVTDNEIDNEGLKSVSK 445
             SI  I +SC  LVSLKMESCSLV REA+ LIG++C  LEELD+TDNEID+EGLKS+S 
Sbjct: 372 RVSITQIANSCPLLVSLKMESCSLVSREAFWLIGQKCRLLEELDLTDNEIDDEGLKSISS 431

Query: 446 CSRLSVLKLGICSNINDDGLLHIASGCPKIKELDLYRSTGITDRGIAAIAGGCPALEMIN 505
           C  LS LKLGIC NI D GL +I  GC  ++ELDLYRS GITD GI+ IA GC  LE IN
Sbjct: 432 CLSLSSLKLGICLNITDKGLSYIGMGCSNLRELDLYRSVGITDVGISTIAQGCIHLETIN 491

Query: 506 IAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSVGLSAIAMGCKQLTVLDIKKCVN--- 565
           I+Y   +TD SL+SLSKC  L+  E RGC  I+S GL+AIA+ CK+L  +D+KKC +   
Sbjct: 492 ISYCQDITDKSLVSLSKCSLLQTFESRGCPNITSQGLAAIAVRCKRLAKVDLKKCPSIND 551

Query: 566 ---------------INLSYCSVTDVGLLSLASINCLRNMTILHLAGLTPNGLTAALLVC 625
                          IN+S  +VT+VGLLSLA+I CL+N+ +++ +GL P+G+ AALL C
Sbjct: 552 AGLLALAHFSQNLKQINVSDTAVTEVGLLSLANIGCLQNIAVVNSSGLRPSGVAAALLGC 611

Query: 626 GTLRKVKLHLSFKSKLPPSFSKYMESHGCVLYWRDKAFQ 645
           G LRK KLH S +S LP S   ++E+ GC   W+D   Q
Sbjct: 612 GGLRKAKLHASLRSLLPLSLIHHLEARGCAFLWKDNTLQ 648

BLAST of MS014175 vs. TAIR 10
Match: AT5G27920.1 (F-box family protein )

HSP 1 Score: 342.8 bits (878), Expect = 6.0e-94
Identity = 210/620 (33.87%), Postives = 354/620 (57.10%), Query Frame = 0

Query: 45  DPFSRKSFSLVCKSFHAIESRQRKSLRPLRSDLTPSIAPRFPSISKLDLSLCHHVEDTFL 104
           DP  RK++ L+ K F  ++S  R ++R LR +  P++  ++P++S LDLS+C  ++D  +
Sbjct: 24  DPPCRKTWRLISKDFLRVDSLTRTTIRILRVEFLPTLLFKYPNLSSLDLSVCPKLDDDVV 83

Query: 105 ISISRAWKMT---LRSVDLSRSRSFTTVGLSSLVSNCAGLVEIDLSNGVALTDSALKVIA 164
           + ++    ++   ++S++LSRS +    GL +L   C  L  +D+S+     D     ++
Sbjct: 84  LRLALDGAISTLGIKSLNLSRSTAVRARGLETLARMCHALERVDVSHCWGFGDREAAALS 143

Query: 165 EAKNLEKLWLARCKSITDMGIGCVAVGCRKLKHLCLNWCLRITDLGVDLIGRKCHELRSL 224
            A  L +L + +C S++D+G+  + VGC  L  + L WC+ I+DLG+DL+ + C  L+SL
Sbjct: 144 SATGLRELKMDKCLSLSDVGLARIVVGCSNLNKISLKWCMEISDLGIDLLCKICKGLKSL 203

Query: 225 DLSFLPITEKCLSTILQLQQLEELVLEGCHGIDD-GLAVLERHCKSNSLKLLNLSHCKSI 284
           D+S+L IT   + +I  L +LE L +  C  IDD GL  LE    S SL+ ++++ C  +
Sbjct: 204 DVSYLKITNDSIRSIALLVKLEVLDMVSCPLIDDGGLQFLEN--GSPSLQEVDVTRCDRV 263

Query: 285 SHIGLSSLINGAQDLQQLILSY-GSSVTTDVAKCLHSFSGLQSIKLDCCSLTSSGVRAIA 344
           S  GL S++ G  D+Q L  S+  S V+    K +     L++I +D   ++ S + +++
Sbjct: 264 SLSGLISIVRGHPDIQLLKASHCVSEVSGSFLKYIKGLKHLKTIWIDGAHVSDSSLVSLS 323

Query: 345 NWRASLKELSLSKCSGVMDECLSILVQKHKQLRKLDITCCRKITFASINSITSSCSSLVS 404
           +   SL E+ LS+C  V D  +  L +    L+ L++ CC  +T  +I+++  SC +L +
Sbjct: 324 SSCRSLMEIGLSRCVDVTDIGMISLARNCLNLKTLNLACCGFVTDVAISAVAQSCRNLGT 383

Query: 405 LKMESCSLVPREAYVLIGERCPFLEELDVTD-NEIDNEGLKSVSKCSRLSVLKLGICSNI 464
           LK+ESC L+  +    +G     ++ELD+TD   +++ GL+ +SKCS L  LKLG+C+NI
Sbjct: 384 LKLESCHLITEKGLQSLGCYSMLVQELDLTDCYGVNDRGLEYISKCSNLQRLKLGLCTNI 443

Query: 465 NDDGLLHIASGCPKIKELDLYRSTGITDRGIAAIAGGCPALEMINIAYNIKVTDSSLISL 524
           +D G+ HI S C K+ ELDLYR  G  D G+AA++ GC +L  + ++Y  ++TD+ +  +
Sbjct: 444 SDKGIFHIGSKCSKLLELDLYRCAGFGDDGLAALSRGCKSLNRLILSYCCELTDTGVEQI 503

Query: 525 SKCLRLKALEIRGCCCISSVGLSAIAMGCKQLTVLDIKKCVN------------------ 584
            +   L  LE+RG   I+ VGL+AIA GCK+L  LD+K C N                  
Sbjct: 504 RQLELLSHLELRGLKNITGVGLAAIASGCKKLGYLDVKLCENIDDSGFWALAYFSKNLRQ 563

Query: 585 INLSYCSVTDVGLLSLAS-INCLRNMTILHLAGLTPNGLTAAL-LVCGTLRKVKLHLSFK 639
           INL  CSV+D  L  L S ++ ++++ ++HL+ +T  G   AL   C  L+K+KL    +
Sbjct: 564 INLCNCSVSDTALCMLMSNLSRVQDVDLVHLSRVTVEGFEFALRACCNRLKKLKLLAPLR 623

BLAST of MS014175 vs. TAIR 10
Match: AT4G15475.1 (F-box/RNI-like superfamily protein )

HSP 1 Score: 183.0 bits (463), Expect = 7.9e-46
Identity = 169/610 (27.70%), Postives = 282/610 (46.23%), Query Frame = 0

Query: 30  LTEEIVFLIVGHLHDDPFSRKSFSLVCKSFHAIESRQRKSLRPLRS----DLTPSIAPRF 89
           L EE++  I   L   P +R + SLVCK + ++E   R +LR   S    D    ++ RF
Sbjct: 11  LPEELILEIFRRLESKP-NRDACSLVCKRWLSLERFSRTTLRIGASFSPDDFISLLSRRF 70

Query: 90  PSISKLDLSLCHHVEDTFLISISRAWKMTLRSVDLSRSRSFTTVGLSSLVSNCAGLVEID 149
             I+ +      HV++   +S+         S    R R   +   SS            
Sbjct: 71  LYITSI------HVDERISVSLPSL------SPSPKRKRGRDSSSPSS------------ 130

Query: 150 LSNGVALTDSALKVIAEAKNLEKLWLARCKSITDMGIGCVAVGCRKLKHLCLNWCLRITD 209
            S    LTD   K  + A+N+E        S+TD G+  +A G  ++++L L WC  ++ 
Sbjct: 131 -SKRKKLTD---KTHSGAENVES------SSLTDTGLTALANGFPRIENLSLIWCPNVSS 190

Query: 210 LGVDLIGRKCHELRSLDLSFLPITEKCLSTILQL-QQLEELVLEGCHGIDD-GLAVLERH 269
           +G+  + +KC  L+SLDL    + ++ L+ + +  +QLEEL L  C G+ D G+  L   
Sbjct: 191 VGLCSLAQKCTSLKSLDLQGCYVGDQGLAAVGKFCKQLEELNLRFCEGLTDVGVIDLVVG 250

Query: 270 CKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLILSYGSSVTTDVAKCLHSFSGLQSI 329
           C S SLK + ++    I+ + L ++ +  + L+ L L         +         L+++
Sbjct: 251 C-SKSLKSIGVAASAKITDLSLEAVGSHCKLLEVLYLDSEYIHDKGLIAVAQGCHRLKNL 310

Query: 330 KLDCCSLTSSGVRAIANWRASLKELSLSKCSGVMDECLSILVQKHKQLRKLDITCCRKIT 389
           KL C S+T     A+     SL+ L+L       D+ +  + +  K+L+ L ++ C  ++
Sbjct: 311 KLQCVSVTDVAFAAVGELCTSLERLALYSFQHFTDKGMRAIGKGSKKLKDLTLSDCYFVS 370

Query: 390 FASINSITSSCSSLVSLKMESCSLVPREAYVLIGERCPFLEELDVT-DNEIDNEGLKSVS 449
              + +I   C  L  +++  C  +       IG+ CP L+EL +     I N  L+ + 
Sbjct: 371 CKGLEAIAHGCKELERVEINGCHNIGTRGIEAIGKSCPRLKELALLYCQRIGNSALQEIG 430

Query: 450 K-CSRLSVLKLGICSNINDDGLLHIASGCPKIKELDLYRSTGITDRGIAAIAGGCPALEM 509
           K C  L +L L  CS I D  +  IA GC  +K+L + R   I ++GI +I   C +L  
Sbjct: 431 KGCKSLEILHLVDCSGIGDIAMCSIAKGCRNLKKLHIRRCYEIGNKGIISIGKHCKSLTE 490

Query: 510 INIAYNIKVTDSSLISLSKCLRLKALEIRGCCCISSVGLSAIAMGCKQLTVLDIKKCVNI 569
           +++ +  KV + +LI++ K   L+ L + GC  IS  G++AIA GC QLT LDI    NI
Sbjct: 491 LSLRFCDKVGNKALIAIGKGCSLQQLNVSGCNQISDAGITAIARGCPQLTHLDISVLQNI 550

Query: 570 N------------------LSYC-SVTDVGLLSLA-SINCLRNMTILHLAGLTPNGLTAA 612
                              LS+C  +TD GL  L      L    +++  G+T  G+   
Sbjct: 551 GDMPLAELGEGCPMLKDLVLSHCHHITDNGLNHLVQKCKLLETCHMVYCPGITSAGVATV 584

BLAST of MS014175 vs. TAIR 10
Match: AT5G23340.1 (RNI-like superfamily protein )

HSP 1 Score: 115.5 bits (288), Expect = 1.5e-25
Identity = 111/410 (27.07%), Postives = 187/410 (45.61%), Query Frame = 0

Query: 30  LTEEIVFLIVGHLHDDPFSRKSFSLVCKSFHAIESRQRKSLRPLRS-DLTPSIAPRFPSI 89
           LT++ +  ++  L  D   ++ F LVCK +  ++S  RK L       +   +A RF  I
Sbjct: 10  LTDDELRWVLSRLDSDK-DKEVFGLVCKRWLNLQSTDRKKLAARAGPHMLRRLASRFTQI 69

Query: 90  SKLDL------SLCHHVEDTFLISISRAWKMTLRSVDLSRSRSFTTVGLSSLVSNCAGLV 149
            +LDL      S    V D+ L  IS  +K  LR ++L   +  T  GL+S +  C  L+
Sbjct: 70  VELDLSQSISRSFYPGVTDSDLAVISEGFKF-LRVLNLHNCKGITDTGLAS-IGRCLSLL 129

Query: 150 E-IDLSNGVALTDSALKVIAE-AKNLEKLWLARCKSITDMGIGCVAVGCRKLKHLCLNWC 209
           + +D+S    L+D  L  +AE   +L  L LA C+ ITD  +  ++  CR L+ L L  C
Sbjct: 130 QFLDVSYCRKLSDKGLSAVAEGCHDLRALHLAGCRFITDESLKSLSERCRDLEALGLQGC 189

Query: 210 LRITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQLQQLEELVLEGCHGIDDGLAVL 269
             ITD G+  + + C +++SLD++      KC +                   D G++ +
Sbjct: 190 TNITDSGLADLVKGCRKIKSLDIN------KCSNV-----------------GDAGVSSV 249

Query: 270 ERHCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLILSYGSSVTTDVAKCLHSFSGL 329
            + C S SLK L L  C  + +  +SSL    ++L+ LI+                    
Sbjct: 250 AKACAS-SLKTLKLLDCYKVGNESISSLAQFCKNLETLIIG------------------- 309

Query: 330 QSIKLDCCSLTSSGVRAIA-NWRASLKELSLSKCSGVMDECLSILVQKHKQLRKLDITCC 389
                 C  ++   +  +A + + SLK L +  C  + D  LS ++++ K L  LDI CC
Sbjct: 310 -----GCRDISDESIMLLADSCKDSLKNLRMDWCLNISDSSLSCILKQCKNLEALDIGCC 368

Query: 390 RKITFASINSITS-SCSSLVSLKMESCSLVPREAYVLIGERCPFLEELDV 429
            ++T  +   + S     L  LK+ +C+ +       + ++C  LE +DV
Sbjct: 370 EEVTDTAFRDLGSDDVLGLKVLKVSNCTKITVTGIGKLLDKCSSLEYIDV 368

BLAST of MS014175 vs. TAIR 10
Match: AT1G80570.3 (RNI-like superfamily protein )

HSP 1 Score: 111.7 bits (278), Expect = 2.2e-24
Identity = 122/493 (24.75%), Postives = 204/493 (41.38%), Query Frame = 0

Query: 28  DHLTEEIVFLIVGHLHDDPFSRKSFSLVCKSFHAIESRQRKSLR-----PLRSDLTPSIA 87
           D L + +V+ I+  LH     R S SL CK F ++++ QR SLR        SD   S+ 
Sbjct: 2   DELPDHLVWDILSKLHTTD-DRNSLSLSCKRFFSLDNEQRYSLRIGCGLVPASDALLSLC 61

Query: 88  PRFPSISKLDLSLCHHVEDTFLISISRAWKMTLRSVDLSRSRSFTTVGLSSLVSNCAGLV 147
            RFP++SK+++             I   W   L        +     GL  L +NC  L 
Sbjct: 62  RRFPNLSKVEI-------------IYSGWMSKL-------GKQVDDQGLLVLTTNCHSLT 121

Query: 148 EIDLSNGVALTDSALKVIAEAKNLEKLWLARCKSITDMGIGCVAVGCRKLKHLCLNWCLR 207
           ++ LS    +TD  +  ++    L  L L     IT  G+  +AVGC+KL+ L L  CL 
Sbjct: 122 DLTLSFCTFITDVGIGHLSSCPELSSLKLNFAPRITGCGVLSLAVGCKKLRRLHLIRCLN 181

Query: 208 ITDLGVDLIGRKCHELRSLDLSFLPITEKCLSTILQLQQLEELVLEGCHGIDDGLAVLER 267
           +  +                        + L    +L+ LEEL ++ C  I +G      
Sbjct: 182 VASV------------------------EWLEYFGKLETLEELCIKNCRAIGEG------ 241

Query: 268 HCKSNSLKLLNLSHCKSISHIGLSSLINGAQDLQQLILSYGSSVTTDVAKCLHSFSGLQS 327
               + +KL N       S   L+SL        + +  Y         K L     L  
Sbjct: 242 ----DLIKLRN-------SWRKLTSLQFEVDANYRYMKVYDQLDVERWPKQLVPCDSLVE 301

Query: 328 IKL-DCCSLTSSGVRAIANWRASLKELSLSKCSGVMDECLSILVQKHKQLRKLDI----- 387
           + L +C      G+  +     +L++L L  C+GV D  +  LVQK   LR + +     
Sbjct: 302 LSLGNCIIAPGRGLACVLRNCKNLEKLHLDMCTGVSDSDIIALVQKASHLRSISLRVPSD 361

Query: 388 -------TCCRKITFASINSITSSCSSLVSLKME-------SCSLVPREAYVLIGERCPF 447
                      ++T  S+++I   CS L S K+        S      +  + + ++CP 
Sbjct: 362 FTLPLLNNITLRLTDESLSAIAQHCSKLESFKISFSDGEFPSLFSFTLQGIITLIQKCP- 421

Query: 448 LEELDVTDNEIDNE-GLKSVSKCSRLSVLKLGICSNINDDGLLHIASGCPKIKELDLYRS 495
           + EL +    + N+ G++++    +L +L+L  C  ++D+GL+ + S  P +  L L + 
Sbjct: 422 VRELSLDHVCVFNDMGMEALCSAQKLEILELVHCQEVSDEGLI-LVSQFPSLNVLKLSKC 430

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149653.10.0e+0096.98F-box/LRR-repeat protein 3-like [Momordica charantia][more]
XP_038902182.10.0e+0084.06F-box/LRR-repeat protein 3-like isoform X1 [Benincasa hispida][more]
XP_038902183.10.0e+0084.04F-box/LRR-repeat protein 3-like isoform X2 [Benincasa hispida][more]
XP_022986786.10.0e+0083.33F-box/LRR-repeat protein 3-like isoform X1 [Cucurbita maxima] >XP_022986788.1 F-... [more]
XP_022986789.15.0e-31083.31F-box/LRR-repeat protein 3-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8RWU55.9e-17954.15F-box/LRR-repeat protein 3 OS=Arabidopsis thaliana OX=3702 GN=FBL3 PE=2 SV=1[more]
Q9C5D21.1e-4427.70F-box/LRR-repeat protein 4 OS=Arabidopsis thaliana OX=3702 GN=FBL4 PE=2 SV=1[more]
Q8NEE61.0e-2924.78Dynein regulatory complex subunit 6 OS=Homo sapiens OX=9606 GN=FBXL13 PE=2 SV=3[more]
Q58DG66.1e-2730.89F-box/LRR-repeat protein 20 OS=Bos taurus OX=9913 GN=FBXL20 PE=2 SV=2[more]
Q96IG26.1e-2730.89F-box/LRR-repeat protein 20 OS=Homo sapiens OX=9606 GN=FBXL20 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1D7C80.0e+0096.98F-box/LRR-repeat protein 3-like OS=Momordica charantia OX=3673 GN=LOC111018032 P... [more]
A0A6J1JHJ80.0e+0083.33F-box/LRR-repeat protein 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1JH242.4e-31083.31F-box/LRR-repeat protein 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1FYI63.1e-30882.58F-box/LRR-repeat protein 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1FV459.0e-30882.56F-box/LRR-repeat protein 3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT5G01720.14.2e-18054.15RNI-like superfamily protein [more]
AT5G27920.16.0e-9433.87F-box family protein [more]
AT4G15475.17.9e-4627.70F-box/RNI-like superfamily protein [more]
AT5G23340.11.5e-2527.07RNI-like superfamily protein [more]
AT1G80570.32.2e-2424.75RNI-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006553Leucine-rich repeat, cysteine-containing subtypeSMARTSM00367LRR_CC_2coord: 164..188
e-value: 51.0
score: 9.0
coord: 470..495
e-value: 2.2E-5
score: 33.9
coord: 85..110
e-value: 37.0
score: 10.1
coord: 522..546
e-value: 200.0
score: 4.3
coord: 445..469
e-value: 140.0
score: 5.6
coord: 138..163
e-value: 68.0
score: 8.1
coord: 266..291
e-value: 3.7
score: 16.5
coord: 394..419
e-value: 340.0
score: 2.6
coord: 547..579
e-value: 12.0
score: 14.0
coord: 215..239
e-value: 65.0
score: 8.2
coord: 189..214
e-value: 0.33
score: 20.0
coord: 112..137
e-value: 85.0
score: 7.3
coord: 317..341
e-value: 200.0
score: 4.4
coord: 368..393
e-value: 0.53
score: 19.3
coord: 420..444
e-value: 12.0
score: 13.8
coord: 342..367
e-value: 30.0
score: 10.8
coord: 496..521
e-value: 0.099
score: 21.7
IPR001611Leucine-rich repeatPFAMPF13516LRR_6coord: 521..544
e-value: 0.024
score: 14.7
coord: 420..441
e-value: 5.6E-4
score: 19.8
coord: 562..577
e-value: 1.8
score: 8.9
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 310..413
e-value: 9.5E-16
score: 59.6
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 22..303
e-value: 5.0E-41
score: 142.7
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 414..623
e-value: 8.9E-37
score: 128.6
NoneNo IPR availablePANTHERPTHR13318:SF37F-BOX/LRR-REPEAT PROTEIN 3-LIKE ISOFORM X1coord: 22..578
NoneNo IPR availablePANTHERPTHR13318UNCHARACTERIZEDcoord: 22..578
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 78..408
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 312..561

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014175.1MS014175.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding