MS014021 (gene) Bitter gourd (TR) v1

BLAST of MS014021 vs. NCBI nr
Match: XP_022149689.1 (RNA polymerase II subunit 5-mediating protein homolog isoform X1 [Momordica charantia])

HSP 1 Score: 688.3 bits (1775), Expect = 3.6e-194
Identity = 372/374 (99.47%), Postives = 373/374 (99.73%), Query Frame = 0

Query: 1   MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL 60
           MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL
Sbjct: 1   MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL 60

Query: 61  PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV 120
           PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV
Sbjct: 61  PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV 120

Query: 121 DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEYEEENSSEREPESGVNKQDVHSVPEV 180
           DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEY EENSSEREPESGVNKQDVHSVPEV
Sbjct: 121 DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEYVEENSSEREPESGVNKQDVHSVPEV 180

Query: 181 DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR 240
           DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR
Sbjct: 181 DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR 240

Query: 241 FSKTSTSPWQREKNIGSEEPLGKYQQQQEASNDPSNCTGLSVQSLAREDTLHGNNSSTQR 300
           FSKTSTSPWQREKNIGSEEPLGKYQQQ+EASNDPSNCTGLSVQSLAREDTLHGNNSSTQR
Sbjct: 241 FSKTSTSPWQREKNIGSEEPLGKYQQQEEASNDPSNCTGLSVQSLAREDTLHGNNSSTQR 300

Query: 301 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 360
           VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS
Sbjct: 301 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 360

Query: 361 QPSKPVSRFKMQRR 375
           QPSKPVSRFKMQRR
Sbjct: 361 QPSKPVSRFKMQRR 374

BLAST of MS014021 vs. NCBI nr
Match: XP_022149690.1 (RNA polymerase II subunit 5-mediating protein homolog isoform X2 [Momordica charantia])

HSP 1 Score: 681.8 bits (1758), Expect = 3.4e-192
Identity = 371/374 (99.20%), Postives = 372/374 (99.47%), Query Frame = 0

Query: 1   MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL 60
           MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL
Sbjct: 1   MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL 60

Query: 61  PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV 120
           PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV
Sbjct: 61  PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV 120

Query: 121 DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEYEEENSSEREPESGVNKQDVHSVPEV 180
           DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEY EENSSEREPESGVNKQDVHSVPEV
Sbjct: 121 DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEYVEENSSEREPESGVNKQDVHSVPEV 180

Query: 181 DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR 240
           DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR
Sbjct: 181 DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR 240

Query: 241 FSKTSTSPWQREKNIGSEEPLGKYQQQQEASNDPSNCTGLSVQSLAREDTLHGNNSSTQR 300
           FSKTSTSPWQREKNIGSEEPLGKYQQQ+EASNDPSNCTGLSVQSLAR DTLHGNNSSTQR
Sbjct: 241 FSKTSTSPWQREKNIGSEEPLGKYQQQEEASNDPSNCTGLSVQSLAR-DTLHGNNSSTQR 300

Query: 301 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 360
           VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS
Sbjct: 301 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 360

Query: 361 QPSKPVSRFKMQRR 375
           QPSKPVSRFKMQRR
Sbjct: 361 QPSKPVSRFKMQRR 373

BLAST of MS014021 vs. NCBI nr
Match: XP_038901192.1 (RNA polymerase II subunit 5-mediating protein homolog [Benincasa hispida] >XP_038901193.1 RNA polymerase II subunit 5-mediating protein homolog [Benincasa hispida])

HSP 1 Score: 528.9 bits (1361), Expect = 3.6e-146
Identity = 298/376 (79.26%), Postives = 330/376 (87.77%), Query Frame = 0

Query: 1   MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL 60
           ME GGAKGTVT L+SLFPA++AHKAA RVQD +SEKQ+EL+ LRGFI+DNANLINLVQKL
Sbjct: 1   MEVGGAKGTVTPLASLFPAQEAHKAAMRVQDAISEKQRELERLRGFITDNANLINLVQKL 60

Query: 61  PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV 120
           PEEL+HEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQ VEILKRRGKALDSQV
Sbjct: 61  PEELHHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQV 120

Query: 121 DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEYEEENSSEREPESGVNKQDVHSVPEV 180
           DSLKAMMD+LKAEASFF ATA+E+ EGLVEIKEEY EEN SE++  SGVNKQDV SV  V
Sbjct: 121 DSLKAMMDNLKAEASFFGATAAESEEGLVEIKEEYVEENFSEQDSGSGVNKQDVPSVSGV 180

Query: 181 DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR 240
           DKAKI EED EYARIMARLDELEKEEELAA N +Q DEEDEEEK TS+QSLEKF+D++  
Sbjct: 181 DKAKIAEEDAEYARIMARLDELEKEEELAAANGDQSDEEDEEEKETSDQSLEKFYDDKQS 240

Query: 241 FSKTSTSPWQREKNIGSEEPLGKYQQQQEASNDPSNCTGLSVQSLAREDTLHGNN--SST 300
           +SK STS W  ++N+GS+E L KYQQQ+EAS + SN +GLS QS ++ED   G N  S T
Sbjct: 241 YSKGSTSAWLMDENVGSKELLDKYQQQREASTNSSNYSGLSEQSSSKEDVSSGKNSLSQT 300

Query: 301 QRVRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPS 360
           QRVRNP+ AAKSVTFAE +EKNQTLPPS++EAFTGSIIERPPII KTSKQETETPLQ PS
Sbjct: 301 QRVRNPNPAAKSVTFAEVKEKNQTLPPSTTEAFTGSIIERPPIIPKTSKQETETPLQ-PS 360

Query: 361 SSQPSKPVSRFKMQRR 375
            SQPSKPVSRFKMQRR
Sbjct: 361 GSQPSKPVSRFKMQRR 375

BLAST of MS014021 vs. NCBI nr
Match: XP_022985987.1 (RNA polymerase II subunit 5-mediating protein homolog [Cucurbita maxima] >XP_022985988.1 RNA polymerase II subunit 5-mediating protein homolog [Cucurbita maxima] >XP_022985989.1 RNA polymerase II subunit 5-mediating protein homolog [Cucurbita maxima])

HSP 1 Score: 527.3 bits (1357), Expect = 1.1e-145
Identity = 301/374 (80.48%), Postives = 330/374 (88.24%), Query Frame = 0

Query: 4   GGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKLPEE 63
           GGAKGTV SLSSLFPA+DAHKAA RVQD +SEKQ+EL+ LRGFISDNANLINLVQKLPEE
Sbjct: 7   GGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLVQKLPEE 66

Query: 64  LYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQVDSL 123
           L+HEVMVPFGKAAFFPGRL+HTNEFLVLLGEGYYAERTSKQ VEILKRRGKALDSQVDSL
Sbjct: 67  LHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVDSL 126

Query: 124 KAMMDDLKAEASFFDATASEAAEGLVEIKEEYEEENSSEREPESGVNKQDVHSVPEVDKA 183
           KAMMDDLKAEASFFDATASEAAEGLVEI EEY EEN+SE+E  SGVNKQD  SV  VD+A
Sbjct: 127 KAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSVSGVDEA 186

Query: 184 KITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELRFSK 243
           +ITEEDEEYARIM+RLDELE+EEELAA NDNQ DEE EEEK TSNQSLEK HD +  +SK
Sbjct: 187 RITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDAKHSYSK 246

Query: 244 TSTSPWQREKNIGSEEPLGKY-QQQQEASNDPSNCTGLSVQSLAREDTLHGNNSSTQR-- 303
            ST P QR+++IGS+E L KY QQQQEAS +PSNC+  S QS + + +LHGNNSSTQ   
Sbjct: 247 ASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQS-SSKGSLHGNNSSTQTRG 306

Query: 304 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 363
           +RN +SAAKSVTFAE +EKNQTLP S+SEAFTGSI+ERPPI+ K SKQETETPLQ PS S
Sbjct: 307 MRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPLQ-PSGS 366

Query: 364 QPSKPVSRFKMQRR 375
           QPSKPVSRFKMQRR
Sbjct: 367 QPSKPVSRFKMQRR 378

BLAST of MS014021 vs. NCBI nr
Match: XP_023512548.1 (RNA polymerase II subunit 5-mediating protein homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 522.7 bits (1345), Expect = 2.6e-144
Identity = 297/374 (79.41%), Postives = 331/374 (88.50%), Query Frame = 0

Query: 4   GGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKLPEE 63
           GGAKGTV +LSSLFPA+DAHKAA RVQD +S+KQ+EL+ LRGFISDNANLINLVQKLPEE
Sbjct: 8   GGAKGTVAALSSLFPAQDAHKAAMRVQDAISDKQRELEHLRGFISDNANLINLVQKLPEE 67

Query: 64  LYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQVDSL 123
           L+HEVMVPFGKAAFFPGRL+HTNEFLVLLGEGYYAERTSKQ VEILKRRGKALDSQVDSL
Sbjct: 68  LHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVDSL 127

Query: 124 KAMMDDLKAEASFFDATASEAAEGLVEIKEEYEEENSSEREPESGVNKQDVHSVPEVDKA 183
           KAMMDDLKAEASFFDATASEAAEGLVEI EEY EE++SE+E  SGVNKQD  SV  VD+A
Sbjct: 128 KAMMDDLKAEASFFDATASEAAEGLVEITEEYVEEDNSEQESGSGVNKQDAPSVSGVDEA 187

Query: 184 KITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELRFSK 243
           +ITEEDEEYARIM+RLDELEKEEELAA N+NQ DEE EEEK TSNQS+EK HD +  +SK
Sbjct: 188 RITEEDEEYARIMSRLDELEKEEELAAANNNQNDEEGEEEKETSNQSMEKLHDAKHSYSK 247

Query: 244 TSTSPWQREKNIGSEEPLGKY-QQQQEASNDPSNCTGLSVQSLAREDTLHGNNSSTQR-- 303
            STSP +R+++IGS+E L KY QQQQEAS +PSNC+  S QS + + +LHGNNSSTQ   
Sbjct: 248 ASTSPCRRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQS-SSKGSLHGNNSSTQTRG 307

Query: 304 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 363
           +RN +SAAKSVTFAE +EKNQTLP S+SEAFTGSI+ERPPI+ K SKQETETPLQ PS S
Sbjct: 308 MRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPLQ-PSGS 367

Query: 364 QPSKPVSRFKMQRR 375
           QPSKPVSRFKMQRR
Sbjct: 368 QPSKPVSRFKMQRR 379

BLAST of MS014021 vs. ExPASy Swiss-Prot
Match: Q54HG4 (RNA polymerase II subunit 5-mediating protein homolog OS=Dictyostelium discoideum OX=44689 GN=rmp PE=3 SV=2)

HSP 1 Score: 99.8 bits (247), Expect = 7.1e-20
Identity = 71/212 (33.49%), Postives = 115/212 (54.25%), Query Frame = 0

Query: 28  RVQDTLSEKQKELDCLRGFISDNANLINLVQKLPEELYHEVMVPFGKAAFFPGRLIHTNE 87
           + +  + E Q  ++ L+   +D   LI+ +Q LP+ L  ++MVP GK AFF G L +TNE
Sbjct: 9   KYEQKVVEYQDHIEELKSTKNDYKQLIDTLQHLPDTLQPKIMVPMGKLAFFEGNLKNTNE 68

Query: 88  FLVLLGEGYYAERTSKQAVEILKRRGKALDSQVDSLKAMMDDLKAEAS----FFDATASE 147
            L+LLG+ Y+A+R+SKQ ++I++RR K +D+ ++ L+  +  LK   S       A   +
Sbjct: 69  ILILLGDNYFAKRSSKQTIDIIQRRDKDIDTSINDLREQIKGLKQRVSMTTDLSKALHEK 128

Query: 148 AAEGLVEIKEEYEEENSSEREPESGVNKQDVHSV------------PEVDKAKITEEDEE 207
             + +VEIKEEY  +   E+E +     Q   +              E +K K  E DEE
Sbjct: 129 EYDNIVEIKEEYNSDEEREKEKKRKQKPQKSTTTTTTTTTSKDKPKTEEEKKKSKEMDEE 188

Query: 208 YARIMARLDELEKEEELAAGNDNQGDEEDEEE 224
           + +++ RL  LE++E      +  GD+ DEEE
Sbjct: 189 FDKMLKRLSILEEKE------NKMGDDYDEEE 214

BLAST of MS014021 vs. ExPASy Swiss-Prot
Match: O94763 (Unconventional prefoldin RPB5 interactor 1 OS=Homo sapiens OX=9606 GN=URI1 PE=1 SV=3)

HSP 1 Score: 79.7 bits (195), Expect = 7.6e-14
Identity = 72/257 (28.02%), Postives = 129/257 (50.19%), Query Frame = 0

Query: 19  AEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKLPEELYHEVMVPFGKAAFF 78
           A D  +     +  ++  Q+ +   +   +D   L   +  LP++L + +MVPFG  AF 
Sbjct: 27  APDVARLREEQEKVVTNCQERIQHWKKVDNDYNALRERLSTLPDKLSYNIMVPFGPFAFM 86

Query: 79  PGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQVDSLKAMMDDLKAEASFFD 138
           PG+L+HTNE  VLLG+ ++A+ ++KQAV +++ R + +   +D LK +M + ++   F +
Sbjct: 87  PGKLVHTNEVTVLLGDNWFAKCSAKQAVGLVEHRKEHVRKTIDDLKKVMKNFESRVEFTE 146

Query: 139 --ATASEAAEGLVEIKEEYE---EENSSEREPESGVNKQDVHSVPEVDKAKITEEDEEYA 198
                S+AA  +V+I+EE +   E  +  R      +K     + E D A   +  +  A
Sbjct: 147 DLQKMSDAAGDIVDIREEIKCDFEFKAKHRIAHKPHSKPKTSDIFEADIANDVKSKDLLA 206

Query: 199 --RIMARLDELEKEEEL------------AAGNDNQGDEEDEEEKGTSNQSLEKFHDEEL 255
              + ARL+ELE++EEL            A G D    EE++E++ T+  ++ +  D   
Sbjct: 207 DKELWARLEELERQEELLGELDSKPDTVIANGEDTTSSEEEKEDRNTNVNAMHQVTDSHT 266

BLAST of MS014021 vs. ExPASy Swiss-Prot
Match: Q3TLD5 (Unconventional prefoldin RPB5 interactor OS=Mus musculus OX=10090 GN=Uri1 PE=1 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 8.5e-13
Identity = 65/201 (32.34%), Postives = 108/201 (53.73%), Query Frame = 0

Query: 48  SDNANLINLVQKLPEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVE 107
           +D + L   ++ LP++L ++VMVPFG  AF PG+L+HTNE  VLLG+ ++A+ ++KQAV 
Sbjct: 54  NDYSALQERLRTLPDKLSYDVMVPFGPLAFMPGKLVHTNEVTVLLGDNWFAKCSAKQAVG 113

Query: 108 ILKRRGKALDSQVDSLKAMMDDLKAEASFFD--ATASEAAEGLVEIKEEYE---EENSSE 167
           +++ R + +   +D  K ++ + ++   F +     S+AA   V+I+EE +   E    +
Sbjct: 114 LVEHRKEHVRKTIDDFKKVLKNFESRVEFTEDLQKMSDAAGDFVDIREEIKSDFEFKGKQ 173

Query: 168 REPESGVNKQDVHSVPEVD-----KAKITEEDEEYARIMARLDELEKEEEL--------- 227
           R      +K     + E D     K K T + +E   + ARL+ELE++EEL         
Sbjct: 174 RIAHKPHSKPKTSDIFEADFENGVKPKNTFDADE---LWARLEELERQEELLGELESKPD 233

BLAST of MS014021 vs. ExPASy Swiss-Prot
Match: Q3B7M7 (Unconventional prefoldin RPB5 interactor OS=Bos taurus OX=9913 GN=URI1 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 4.2e-12
Identity = 71/255 (27.84%), Postives = 126/255 (49.41%), Query Frame = 0

Query: 1   MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL 60
           MEA        S ++   A +  +     +  ++  Q+++   +   +D   L   +  L
Sbjct: 1   MEAPPDPRPHASAAAPLRAPEVARLREEQEKVVTNCQEKIQHWKKVDNDYNALQERLSTL 60

Query: 61  PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV 120
           P++L + +MVPFG  AF PG+L+HTNE  VLLG+ ++A+ ++KQAV +++ R + +   +
Sbjct: 61  PDKLSYNIMVPFGPFAFMPGKLVHTNEVTVLLGDNWFAKCSAKQAVGLVEHRKEHVRKTI 120

Query: 121 DSLKAMMDDLKAEASFFD--ATASEAAEGLVEIKE------EYEEENSSEREPESGVNKQ 180
           D LK +M + ++   F +     S+AA  +V+I+E      E++ ++    +P S     
Sbjct: 121 DDLKKVMKNFESRVEFTEDLQKMSDAAGDIVDIREEIKTDFEFKAKHRIAHKPHSKPKTS 180

Query: 181 DVHSVPEVD--KAKITEEDEEYARIMARLDELEKEEELAAGND----------NQGDEED 236
           D+      +  K+K    D+E   +  RL+ELE++EEL    D          N  D   
Sbjct: 181 DIFEAEFANDLKSKDLLADKE---LWDRLEELERQEELLGEIDIDSKPDTVIANGEDVSS 240

BLAST of MS014021 vs. ExPASy Swiss-Prot
Match: Q9W148 (Unconventional prefoldin RPB5 interactor-like protein OS=Drosophila melanogaster OX=7227 GN=uri PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.0e-06
Identity = 65/216 (30.09%), Postives = 102/216 (47.22%), Query Frame = 0

Query: 33  LSEKQKELDCLRGFISDNANLINLVQKLPEELYHEVMVPFGKAAFFPGRLIHTNEFLVLL 92
           L     E +    F  DN + I  + K  + L  EVMVP G+ A  PG LIHTNE LV  
Sbjct: 12  LQTNASETERWEAFKRDNESTIRNLDKFAKNLSVEVMVPIGRKALMPGELIHTNELLVGH 71

Query: 93  GEGYYAERTSKQAVEILKRRGKALDSQVDSLKAMMDDL---KAEASFFDATASEAAEGLV 152
            EGY++  +S +A EI + R K  + Q+  L A+ +DL   K    F +       +  V
Sbjct: 72  YEGYFSACSSHKAKEICQYRLKLAEEQLKKL-AVENDLWQKKLHTPFAEGAVPSGDQ--V 131

Query: 153 EIKEEYEEENSSEREPESGVNKQDVHSVPEVDK-AKITEEDEEYARIMARLDELEKEEEL 212
           EI E++ EE+ ++   E     +       +++ A+  ++D E   ++ +L+E E  EEL
Sbjct: 132 EIVEDFNEESHNKWLAEHRKRMRQQKQKERLEREAEPVKKDNE---VLRKLEEREMMEEL 191

Query: 213 AAGNDNQGDEE------DEEEKGTSNQSLEKFHDEE 239
               DN  +++       E  K T+  S +    EE
Sbjct: 192 GLDPDNIDEDQLHDMLNQEPLKSTNESSPKSLTQEE 221

BLAST of MS014021 vs. ExPASy TrEMBL
Match: A0A6J1D7S6 (RNA polymerase II subunit 5-mediating protein homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018055 PE=4 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 1.7e-194
Identity = 372/374 (99.47%), Postives = 373/374 (99.73%), Query Frame = 0

Query: 1   MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL 60
           MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL
Sbjct: 1   MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL 60

Query: 61  PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV 120
           PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV
Sbjct: 61  PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV 120

Query: 121 DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEYEEENSSEREPESGVNKQDVHSVPEV 180
           DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEY EENSSEREPESGVNKQDVHSVPEV
Sbjct: 121 DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEYVEENSSEREPESGVNKQDVHSVPEV 180

Query: 181 DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR 240
           DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR
Sbjct: 181 DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR 240

Query: 241 FSKTSTSPWQREKNIGSEEPLGKYQQQQEASNDPSNCTGLSVQSLAREDTLHGNNSSTQR 300
           FSKTSTSPWQREKNIGSEEPLGKYQQQ+EASNDPSNCTGLSVQSLAREDTLHGNNSSTQR
Sbjct: 241 FSKTSTSPWQREKNIGSEEPLGKYQQQEEASNDPSNCTGLSVQSLAREDTLHGNNSSTQR 300

Query: 301 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 360
           VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS
Sbjct: 301 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 360

Query: 361 QPSKPVSRFKMQRR 375
           QPSKPVSRFKMQRR
Sbjct: 361 QPSKPVSRFKMQRR 374

BLAST of MS014021 vs. ExPASy TrEMBL
Match: A0A6J1D6F4 (RNA polymerase II subunit 5-mediating protein homolog isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018055 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.6e-192
Identity = 371/374 (99.20%), Postives = 372/374 (99.47%), Query Frame = 0

Query: 1   MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL 60
           MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL
Sbjct: 1   MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL 60

Query: 61  PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV 120
           PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV
Sbjct: 61  PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV 120

Query: 121 DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEYEEENSSEREPESGVNKQDVHSVPEV 180
           DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEY EENSSEREPESGVNKQDVHSVPEV
Sbjct: 121 DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEYVEENSSEREPESGVNKQDVHSVPEV 180

Query: 181 DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR 240
           DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR
Sbjct: 181 DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR 240

Query: 241 FSKTSTSPWQREKNIGSEEPLGKYQQQQEASNDPSNCTGLSVQSLAREDTLHGNNSSTQR 300
           FSKTSTSPWQREKNIGSEEPLGKYQQQ+EASNDPSNCTGLSVQSLAR DTLHGNNSSTQR
Sbjct: 241 FSKTSTSPWQREKNIGSEEPLGKYQQQEEASNDPSNCTGLSVQSLAR-DTLHGNNSSTQR 300

Query: 301 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 360
           VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS
Sbjct: 301 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 360

Query: 361 QPSKPVSRFKMQRR 375
           QPSKPVSRFKMQRR
Sbjct: 361 QPSKPVSRFKMQRR 373

BLAST of MS014021 vs. ExPASy TrEMBL
Match: A0A6J1J6D1 (RNA polymerase II subunit 5-mediating protein homolog OS=Cucurbita maxima OX=3661 GN=LOC111483860 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 5.1e-146
Identity = 301/374 (80.48%), Postives = 330/374 (88.24%), Query Frame = 0

Query: 4   GGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKLPEE 63
           GGAKGTV SLSSLFPA+DAHKAA RVQD +SEKQ+EL+ LRGFISDNANLINLVQKLPEE
Sbjct: 7   GGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLVQKLPEE 66

Query: 64  LYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQVDSL 123
           L+HEVMVPFGKAAFFPGRL+HTNEFLVLLGEGYYAERTSKQ VEILKRRGKALDSQVDSL
Sbjct: 67  LHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVDSL 126

Query: 124 KAMMDDLKAEASFFDATASEAAEGLVEIKEEYEEENSSEREPESGVNKQDVHSVPEVDKA 183
           KAMMDDLKAEASFFDATASEAAEGLVEI EEY EEN+SE+E  SGVNKQD  SV  VD+A
Sbjct: 127 KAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSVSGVDEA 186

Query: 184 KITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELRFSK 243
           +ITEEDEEYARIM+RLDELE+EEELAA NDNQ DEE EEEK TSNQSLEK HD +  +SK
Sbjct: 187 RITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDAKHSYSK 246

Query: 244 TSTSPWQREKNIGSEEPLGKY-QQQQEASNDPSNCTGLSVQSLAREDTLHGNNSSTQR-- 303
            ST P QR+++IGS+E L KY QQQQEAS +PSNC+  S QS + + +LHGNNSSTQ   
Sbjct: 247 ASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQS-SSKGSLHGNNSSTQTRG 306

Query: 304 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 363
           +RN +SAAKSVTFAE +EKNQTLP S+SEAFTGSI+ERPPI+ K SKQETETPLQ PS S
Sbjct: 307 MRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPLQ-PSGS 366

Query: 364 QPSKPVSRFKMQRR 375
           QPSKPVSRFKMQRR
Sbjct: 367 QPSKPVSRFKMQRR 378

BLAST of MS014021 vs. ExPASy TrEMBL
Match: A0A6J1FUU9 (RNA polymerase II subunit 5-mediating protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111448325 PE=4 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.4e-143
Identity = 296/374 (79.14%), Postives = 329/374 (87.97%), Query Frame = 0

Query: 4   GGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKLPEE 63
           GGAKGTV +LSSLFPA+DAHKAA RVQD +SEKQ+EL+ L GFISDNANLINLVQKLPEE
Sbjct: 8   GGAKGTVAALSSLFPAQDAHKAAMRVQDAISEKQRELEHLLGFISDNANLINLVQKLPEE 67

Query: 64  LYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQVDSL 123
           L+HEVMVPFGKAAFFPGRL+HTNEFLVLLGEGYYAERTSKQ VEILKRRGKALDSQVDSL
Sbjct: 68  LHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVDSL 127

Query: 124 KAMMDDLKAEASFFDATASEAAEGLVEIKEEYEEENSSEREPESGVNKQDVHSVPEVDKA 183
           KAMMDDLKAEASFFDATASEAAEGLVEI EEY EE++SE+E  SGVNKQD  SV  VD+A
Sbjct: 128 KAMMDDLKAEASFFDATASEAAEGLVEITEEYVEEDNSEQESGSGVNKQDAPSVSGVDEA 187

Query: 184 KITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELRFSK 243
           +ITEEDEEYARIM+RLDELEKEEELAA NDNQ DE+ EEE  TSNQSLEK HD +  +SK
Sbjct: 188 RITEEDEEYARIMSRLDELEKEEELAAANDNQNDEKGEEENETSNQSLEKLHDAKHSYSK 247

Query: 244 TSTSPWQREKNIGSEEPLGKY-QQQQEASNDPSNCTGLSVQSLAREDTLHGNNSSTQR-- 303
            STSP +R+++IGS+E L KY QQQQEAS +PSNC+  S QS + + +LHGN+SSTQ   
Sbjct: 248 ASTSPCRRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQS-SSKGSLHGNSSSTQTRG 307

Query: 304 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 363
           +RN +SAAKSVTFAE +EKNQTLP S+SEAFTGSI+ERPPI+ K SKQETETPLQ PS S
Sbjct: 308 MRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIVPKISKQETETPLQ-PSGS 367

Query: 364 QPSKPVSRFKMQRR 375
           QPSKPVSRFKMQRR
Sbjct: 368 QPSKPVSRFKMQRR 379

BLAST of MS014021 vs. ExPASy TrEMBL
Match: A0A0A0KB27 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G127400 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 3.1e-143
Identity = 291/376 (77.39%), Postives = 324/376 (86.17%), Query Frame = 0

Query: 1   MEAGGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKL 60
           ME+G  KGTVTSL+SLFPA+DA KAA+RVQD +SEKQ+EL+ LRGFI+DN NLI LVQKL
Sbjct: 82  MESGRVKGTVTSLASLFPAQDARKAASRVQDAISEKQRELEQLRGFITDNDNLIKLVQKL 141

Query: 61  PEELYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQV 120
           PEEL+HEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQ  EILKRRGKALDSQV
Sbjct: 142 PEELHHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQTAEILKRRGKALDSQV 201

Query: 121 DSLKAMMDDLKAEASFFDATASEAAEGLVEIKEEYEEENSSEREPESGVNKQDVHSVPEV 180
           DSLKAMMD+LKAEASFFDATASEA +GLVEIKEEY EEN  E+E  SGVNKQDV SV  V
Sbjct: 202 DSLKAMMDNLKAEASFFDATASEAEDGLVEIKEEYVEENFCEQESTSGVNKQDVPSVSGV 261

Query: 181 DKAKITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELR 240
           DKAKI E D EYAR+MAR DELEKEEELAA N N+ D+EDEEEKGT NQSLE+F+ ++  
Sbjct: 262 DKAKIAEIDAEYARMMARFDELEKEEELAAANGNKSDDEDEEEKGTQNQSLERFYGDKQS 321

Query: 241 FSKTSTSPWQREKNIGSEEPLGKYQQQQEASNDPSNCTGLSVQSLAREDTLHGNNSST-- 300
            SK STS W R++N+ S+E L KYQ+QQEAS +PSNC+GLSVQ+  +ED   GNNS T  
Sbjct: 322 LSKGSTSTWPRDENVSSKELLNKYQKQQEASTNPSNCSGLSVQTSPKEDVTSGNNSLTES 381

Query: 301 QRVRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPS 360
           QRV NP+ AAKSVTFAE +EKNQTLPPS+++AFTGSIIERPPII KTSKQETET LQ PS
Sbjct: 382 QRVINPNPAAKSVTFAEVKEKNQTLPPSTNQAFTGSIIERPPIIPKTSKQETETTLQ-PS 441

Query: 361 SSQPSKPVSRFKMQRR 375
            SQPSKPVSRFKMQRR
Sbjct: 442 GSQPSKPVSRFKMQRR 456

BLAST of MS014021 vs. TAIR 10
Match: AT1G03760.1 (Prefoldin chaperone subunit family protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.6e-54
Identity = 161/397 (40.55%), Postives = 240/397 (60.45%), Query Frame = 0

Query: 6   AKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKLPEELY 65
           AKGTVT L   F  ED  KAA+ +++ + EK+ E++ L+ ++ +N NLINLV+KLP++L+
Sbjct: 5   AKGTVTPLG--FSEEDVRKAASYMEEKIGEKRVEMNRLQQYVDENDNLINLVKKLPDQLH 64

Query: 66  HEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQVDSLKA 125
           H VMVPFGK AFFPGRLIHTNE LVLLGE YY +R+SKQ V+ LKRR K L SQ+ SLKA
Sbjct: 65  HNVMVPFGKMAFFPGRLIHTNECLVLLGENYYTDRSSKQTVDFLKRRDKTLQSQIHSLKA 124

Query: 126 MMDDLKAEASFFDATASEAAEGLVEIKEEYEEENSSEREPESGVNKQDVH-SVPEVDKAK 185
            ++DL+ EASFF  TASE A+GLVEI+EEY EE+SS         K+  + S  E ++ +
Sbjct: 125 EIEDLQTEASFFTTTASEVADGLVEIREEYVEEDSSAPVVIHSSEKEPCNLSEGETEEGE 184

Query: 186 ITEEDEEYARIMARLDELEKEEEL-AAGNDNQGDEEDEE--------------EKGTSNQ 245
           +  ED+++ARIMARL+ELE EEEL     D++G++ D                 +G +++
Sbjct: 185 L--EDDDFARIMARLNELEIEEELEGEDGDSRGEDPDSSVESVEQIQHDLVKGSRGETDR 244

Query: 246 SLEKFHDEEL------RFSKTSTSPWQREKNIGSE--EPLGKYQQQQEASNDPSNCTGLS 305
              ++  +E       + S  S+SP   E  + ++  + L +    ++  +DP N     
Sbjct: 245 GRVEYGKQETTISVPNKASGHSSSPRVSEPRVEAKIIQVLPETHPHKDL-DDPLNRIRPM 304

Query: 306 VQSLAREDTLHGN--NSSTQRVRNPSSAAKSVTFAEAREKNQTLPP--SSSEAFTGSIIE 365
            Q L++E    G     + +  ++  + AK+      + + Q   P   S++AFTGSI+E
Sbjct: 305 AQYLSKEGQSRGGTPQQNAETWKDFRTNAKTNVLGPQKIEPQKPEPEFDSTKAFTGSIVE 364

Query: 366 RPPIISKTSKQETETPLQMPSSSQPSKPVSRFKMQRR 375
              I+  ++  +T++     S+SQPSKPVSRF+ +RR
Sbjct: 365 HAHILETSAHSQTQS-----STSQPSKPVSRFRAKRR 391