MS013992 (gene) Bitter gourd (TR) v1

BLAST of MS013992 vs. NCBI nr
Match: XP_022151510.1 (protein NRT1/ PTR FAMILY 2.8-like [Momordica charantia])

HSP 1 Score: 1138.6 bits (2944), Expect = 0.0e+00
Identity = 576/585 (98.46%), Postives = 579/585 (98.97%), Query Frame = 0

Query: 1   MENDAIDHSSLDPPPIAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60
           MENDAIDHSSLDPPP+APSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL
Sbjct: 1   MENDAIDHSSLDPPPVAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60

Query: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPEL 120
           DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASF GMGVLTLGAGLPEL
Sbjct: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFFGMGVLTLGAGLPEL 120

Query: 121 RPPSCSHSQAKCPQPQLWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180
           RPPSCSHSQAKCPQPQ WQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL
Sbjct: 121 RPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180

Query: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240
           ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT
Sbjct: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240

Query: 241 PQGSVFSDMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300
           PQGSVF+DMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV
Sbjct: 241 PQGSVFADMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300

Query: 301 DPAAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360
           DP AELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ
Sbjct: 301 DPVAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360

Query: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420
           MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM
Sbjct: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420

Query: 421 SVVCMVVSGITEKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHL 480
           SVVCMVVSGITEKLRRDLAVGNGSFVA LHVTAL PEFALSGLTEAFAAIAVMELLTTHL
Sbjct: 421 SVVCMVVSGITEKLRRDLAVGNGSFVARLHVTALXPEFALSGLTEAFAAIAVMELLTTHL 480

Query: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540
           PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY
Sbjct: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540

Query: 541 VVAVIGTLNFFYFRFFAAGFLPKGDVDDHDQTHERQLEDEETGRR 586
           VVAVIGTLNFFYFRFFAAGFLPK +VDDHDQTHERQLEDEETGRR
Sbjct: 541 VVAVIGTLNFFYFRFFAAGFLPKSBVDDHDQTHERQLEDEETGRR 585

BLAST of MS013992 vs. NCBI nr
Match: XP_038881205.1 (protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida] >XP_038881206.1 protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida])

HSP 1 Score: 871.3 bits (2250), Expect = 4.7e-249
Identity = 439/578 (75.95%), Postives = 493/578 (85.29%), Query Frame = 0

Query: 8   HSSLDPPPIAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASAN 67
           HSS D P     K A GGWRAVRFIL NETFEKLASMSL+ NL+LYLHTMYNLDNVASA 
Sbjct: 7   HSSSDAP---RKKTARGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAY 66

Query: 68  VINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPELRPPSCSH 127
           V  IWGGTTNFAP+AGAFLADAYLGRFYTLLFGSIASFLGMGVL+LGAGLP++RPP C  
Sbjct: 67  VFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLSLGAGLPQMRPPPCIS 126

Query: 128 SQAKCPQPQLWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKLESFLNWW 187
            ++ CPQPQ WQL FLYLGLGL+V+GAGGIRPCNISFGADQFDT T KGRAKLESFLNWW
Sbjct: 127 GESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWW 186

Query: 188 YFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKTPQGSVFS 247
           Y LFSIAL++ALT+VVY+QTNISW  GFAIPTACFV+SILIFL GR  Y+ K PQGSVFS
Sbjct: 187 YLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIFLSGRRFYVCKAPQGSVFS 246

Query: 248 DMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVVDPAAELD 307
           D+AKV++A  RK K+ KPN  ++LH+PP    +S KL HT+RFLVFDKAATVVD + ELD
Sbjct: 247 DLAKVVVAMFRKRKIPKPNQIDHLHNPP---MNSCKLAHTDRFLVFDKAATVVDSSIELD 306

Query: 308 EHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQMDRFLGR 367
           E G S   WRLCSV QVEQ KCV+GI+PVW+AGI+CFI+MQQMGSFG+LQAIQM+RF+G 
Sbjct: 307 EEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQMGSFGILQAIQMNRFIGP 366

Query: 368 HFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVMSVVCMVV 427
           HF+IPPAWM+LTPMIALSIWI++YEKYV  MK+KT  NKR SM+ RIEIGIVMSV+CMVV
Sbjct: 367 HFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSMKTRIEIGIVMSVLCMVV 426

Query: 428 SGITEKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHLPESLRTV 487
           +G+ EKLRRD AV N SFV+PLHV  L+PEFALSGLTEAFAAIAVMELLTTHLPESLRTV
Sbjct: 427 AGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTV 486

Query: 488 AGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFYVVAVIGT 547
           AGAIFFLSLSLASYLSSVL+GI+SKI   ++  +  W+GGNDLN NRL+YFFYVV  I  
Sbjct: 487 AGAIFFLSLSLASYLSSVLMGIVSKIG-RMEAGEMQWLGGNDLNKNRLDYFFYVVGGIAA 546

Query: 548 LNFFYFRFFAAGFLPKGDVDDHDQTHERQLEDEETGRR 586
           LNFFYFRFFAAGFLP  DVD   + H+RQLED E GRR
Sbjct: 547 LNFFYFRFFAAGFLPSPDVDPRQKAHQRQLEDAELGRR 577

BLAST of MS013992 vs. NCBI nr
Match: XP_023518809.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 856.7 bits (2212), Expect = 1.2e-244
Identity = 438/585 (74.87%), Postives = 498/585 (85.13%), Query Frame = 0

Query: 1   MENDAIDHSSLDPPPIAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60
           M+N  +  SSLDP P    K  +GGWRAVRFILANETFEKLASMSL+ANLILYLHTMYNL
Sbjct: 1   MDNGTL-RSSLDPSP----KPPLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNL 60

Query: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPEL 120
           DNV SA V  IWGGTTNF+P+AGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGA LPE+
Sbjct: 61  DNVDSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEM 120

Query: 121 RPPSCSHSQAKCPQPQLWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180
           RPP CS  +  C Q + WQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDT T KGRAKL
Sbjct: 121 RPPPCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKL 180

Query: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240
           ESFLNWWYFLFSIAL++ALT+VVYIQTNISW  GFAIPTA F+ SI+IFLLGR  YI + 
Sbjct: 181 ESFLNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRI 240

Query: 241 PQGSVFSDMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300
           PQGSVF+DMAKV+IAT RK ++ +P + N+LHDPP    DS+KLPHTNRFLVFDKAA V+
Sbjct: 241 PQGSVFADMAKVLIATYRKRQIPRP-SPNHLHDPP---MDSSKLPHTNRFLVFDKAAMVI 300

Query: 301 DPAAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360
           D + ELDE G S + W+LC+V QVEQLKCV+GIVP+W AGISCFI+MQQMGSFG+LQAIQ
Sbjct: 301 DSSMELDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQ 360

Query: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420
           M+RFLG HFEIPPAWM+L PMIALS+WI++YEKYV  MKRKT  NKR SM+ RIEIGIVM
Sbjct: 361 MNRFLGPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMKRKTHHNKRFSMKMRIEIGIVM 420

Query: 421 SVVCMVVSGITEKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHL 480
           SV+CMVV+GI EK+RRD A+ N +FV+PLHV+ L+PEFALSGLTEAFAAI+VMELLTT L
Sbjct: 421 SVLCMVVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQL 480

Query: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540
           PESLRTVAGAIFFLSLSLASYLSSVL+GI++++S T D  KT+W+ GNDLN N L+YFFY
Sbjct: 481 PESLRTVAGAIFFLSLSLASYLSSVLMGIVNRVSKTGD-SKTSWLEGNDLNKNHLDYFFY 540

Query: 541 VVAVIGTLNFFYFRFFAAGFLPKGDVDDHDQTHERQLEDEETGRR 586
           VV VI  LNFFYFRF    FLP+GDVD   +  ER+ +DEE+G R
Sbjct: 541 VVGVIAALNFFYFRFVGTRFLPEGDVDPRGKAGERRSDDEESGFR 575

BLAST of MS013992 vs. NCBI nr
Match: KAG6595347.1 (Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027355.1 Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 855.9 bits (2210), Expect = 2.0e-244
Identity = 437/585 (74.70%), Postives = 498/585 (85.13%), Query Frame = 0

Query: 1   MENDAIDHSSLDPPPIAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60
           M+N  +  SSLDP P    K  +GGWRAVRFILANETFEKLASMSL+ANLILYLHTMYNL
Sbjct: 1   MDNGTL-RSSLDPSP----KPPLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNL 60

Query: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPEL 120
           DNV SA V  IWGGTTNF+P+AGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGA LPE+
Sbjct: 61  DNVDSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEM 120

Query: 121 RPPSCSHSQAKCPQPQLWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180
           RPP CS  +  C Q + WQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDT T KGRAKL
Sbjct: 121 RPPPCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKL 180

Query: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240
           ESFLNWWYFLFSIAL++ALT+VVYIQTNISW  GFAIPTA F+ SI+IFLLGR  YI + 
Sbjct: 181 ESFLNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRI 240

Query: 241 PQGSVFSDMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300
           PQGSVF+DMAKV+IAT RK ++ +P + N+LHDPP    DS+KLPHTNRFLVFDKAA V+
Sbjct: 241 PQGSVFADMAKVLIATYRKRQIPRP-SPNHLHDPP---MDSSKLPHTNRFLVFDKAAMVI 300

Query: 301 DPAAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360
           D + ELDE G S + W+LC+V QVEQLKCV+GIVP+W AGISCFI+MQQMGSFG+LQAIQ
Sbjct: 301 DSSMELDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQ 360

Query: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420
           M+RFLG HFEIPPAWM+L PMIALS+WI++YEKYV  M+RKT  NKR SM+ RIEIGIVM
Sbjct: 361 MNRFLGPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVM 420

Query: 421 SVVCMVVSGITEKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHL 480
           SV+CM+V+GI EK+RRD A+ N +FV+PLHV+ L+PEFALSGLTEAFAAI+VMELLTT L
Sbjct: 421 SVLCMIVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQL 480

Query: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540
           PESLRTVAGAIFFLSLSLASYLSSVL+GI++++S T D  KT+W+ GNDLN N L+YFFY
Sbjct: 481 PESLRTVAGAIFFLSLSLASYLSSVLMGIVNRVSKTGD-SKTSWLEGNDLNKNHLDYFFY 540

Query: 541 VVAVIGTLNFFYFRFFAAGFLPKGDVDDHDQTHERQLEDEETGRR 586
           VV VI  LNFFYFRF    FLP+GDVD   +  ER+ EDEE+G R
Sbjct: 541 VVGVIAALNFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGFR 575

BLAST of MS013992 vs. NCBI nr
Match: XP_022931895.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita moschata])

HSP 1 Score: 855.9 bits (2210), Expect = 2.0e-244
Identity = 437/585 (74.70%), Postives = 498/585 (85.13%), Query Frame = 0

Query: 1   MENDAIDHSSLDPPPIAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60
           M+N  +  SSLDP P    K  +GGWRAVRFILANETFEKLASMSL+ANLILYLHTMYNL
Sbjct: 1   MDNGTL-RSSLDPSP----KPQLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNL 60

Query: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPEL 120
           DNV SA V  IWGGTTNF+P+AGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGA LPE+
Sbjct: 61  DNVDSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEM 120

Query: 121 RPPSCSHSQAKCPQPQLWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180
           RPP CS  +  C Q + WQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDT T KGRAKL
Sbjct: 121 RPPPCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKL 180

Query: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240
           ESFLNWWYFLFSIAL++ALT+VVYIQTNISW  GFAIPTA F+ SI+IFLLGR  YI + 
Sbjct: 181 ESFLNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRI 240

Query: 241 PQGSVFSDMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300
           PQGSVF+DMAKV+IAT RK ++ +P + N+LHDPP    DS+KLPHTNRFLVFDKAA V+
Sbjct: 241 PQGSVFADMAKVLIATYRKRQIPRP-SPNHLHDPP---MDSSKLPHTNRFLVFDKAAMVI 300

Query: 301 DPAAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360
           D + ELDE G S + W+LC+V QVEQLKCV+GIVP+W AGISCFI+MQQMGSFG+LQAIQ
Sbjct: 301 DSSMELDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQ 360

Query: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420
           M+RFLG HFEIPPAWM+L PMIALS+WI++YEKYV  M+RKT  NKR SM+ RIEIGIVM
Sbjct: 361 MNRFLGPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVM 420

Query: 421 SVVCMVVSGITEKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHL 480
           SV+CM+V+GI EK+RRD A+ N +FV+PLHV+ L+PEFALSGLTEAFAAI+VMELLTT L
Sbjct: 421 SVLCMIVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQL 480

Query: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540
           PESLRTVAGAIFFLSLSLASYLSSVL+GI++++S T D  KT+W+ GNDLN N L+YFFY
Sbjct: 481 PESLRTVAGAIFFLSLSLASYLSSVLMGIVNRVSKTGD-SKTSWLEGNDLNKNHLDYFFY 540

Query: 541 VVAVIGTLNFFYFRFFAAGFLPKGDVDDHDQTHERQLEDEETGRR 586
           VV VI  LNFFYFRF    FLP+GDVD   +  ER+ EDEE+G R
Sbjct: 541 VVGVIAALNFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGFR 575

BLAST of MS013992 vs. ExPASy Swiss-Prot
Match: Q3E8X3 (Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2)

HSP 1 Score: 575.1 bits (1481), Expect = 9.1e-163
Identity = 301/558 (53.94%), Postives = 395/558 (70.79%), Query Frame = 0

Query: 6   IDHSSLDPPPIAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVAS 65
           +D  S  P   A  K   GGWRA+++I+ANE+FEKLASMSL+ NL +YL T YNL  V  
Sbjct: 1   MDVESSSPSSHALIKKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFL 60

Query: 66  ANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPELRPPSC 125
            NVINIW G+ N   +AGAF++DAYLGRF+TLL GSIASF+GMG+  L A LP LRP +C
Sbjct: 61  VNVINIWFGSCNILTLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDAC 120

Query: 126 SHSQAKCPQPQLWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKLESFLN 185
                   QP  WQL  L+ GLGLL IGAGG+RPCNI+FGADQFDT+T KG+A LE+F N
Sbjct: 121 IDPSNCSNQPAKWQLGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFN 180

Query: 186 WWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKTPQGSV 245
           WWYF F++AL++ALT VVYIQTNISWV GF IPTAC  LSI  F++G+ TYI    +GSV
Sbjct: 181 WWYFSFTVALVIALTGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSV 240

Query: 246 FSDMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPH-TNRFLVFDKAATVVDPAA 305
           F+D+ KV+ A C+K K+ KP +    +  P      T L    +R   FDKA+ V +P  
Sbjct: 241 FADIVKVVTAACKKRKV-KPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKASIVTNP-N 300

Query: 306 ELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQMDRF 365
           EL+E GN+   WRLCSVQQV+ LKCV  I+PVWV GI+CFI   Q   +G+LQA+QMD+ 
Sbjct: 301 ELNEDGNAKYKWRLCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKT 360

Query: 366 LGRH-FEIPPAWMNLTPMIALSIWIFVYE-KYVAMMKRKTGQNKRISMQRRIEIGIVMSV 425
            G H F++P  WMNL  MI L+IWI +YE   + ++K+ TG+ KR++++ RIE  IVM +
Sbjct: 361 FGPHNFQVPAGWMNLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGI 420

Query: 426 VCMVVSGITEKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHLPE 485
           +CM+V+G  EK RR  A+ NGSFV+P+ +  L+P+FAL+GLTEAF+A+A+ME LT  +PE
Sbjct: 421 ICMIVAGFQEKKRRASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPE 480

Query: 486 SLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFYVV 545
            +R VAGAIFFLS S+ASY+ ++L+ +I  + T  +GK  +W+G  DLN NRLE +F+++
Sbjct: 481 HMRAVAGAIFFLSSSIASYICTLLINVIDAV-TRKEGK--SWLGDKDLNKNRLENYFFII 540

Query: 546 AVIGTLNFFYFRFFAAGF 561
           A I   N  YFR FA+ +
Sbjct: 541 AGIQVANLLYFRLFASRY 551

BLAST of MS013992 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 4.7e-119
Identity = 236/565 (41.77%), Postives = 349/565 (61.77%), Query Frame = 0

Query: 24  GGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWGGTTNFAPIAG 83
           GGWRAV FIL NET E+L S+ L+AN ++YL  +++L+ V +ANVINIW G TN  P+ G
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 84  AFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPELRPPSC-SHSQAKCPQPQLWQLAF 143
           A+++D Y+GRF T+ F S A+ LG+  +TL A  P+L P SC S     C  P   Q+  
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGV 172

Query: 144 LYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKLESFLNWWYFLFSIALIVALTVV 203
           L LGL  L +G+GGIRPC+I FG DQFD  T +G   + SF NW+Y  F++ LI+  TVV
Sbjct: 173 LLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVV 232

Query: 204 VYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKTPQGSVFSDMAKVIIATCRKSKL 263
           VYIQ  +SW+ GF+IPT    L++++F  G   Y+   P+GS+FS +A+VI+A  +K KL
Sbjct: 233 VYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKL 292

Query: 264 SKPNNQN---NLHDPPPTIPDSTKLPHTNRFLVFDKAATVVDPAAELDEHGNSVSHWRLC 323
             P   +     +DP       +KL  +N+F   DKAA V++   +L   G     WRLC
Sbjct: 293 KLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIE--GDLTPEGPPADKWRLC 352

Query: 324 SVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQMDRFLGRHFEIPPAWMNLT 383
           SVQ+VE++KC+I IVP+W AGI    AM   G+F V QA++MDR LG  FEIP   +++ 
Sbjct: 353 SVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVI 412

Query: 384 PMIALSIWIFVYEK-YVAMMKRKTGQNKRISMQRRIEIGIVMSVVCMVVSGITEKLR--R 443
            ++ + I++  Y++ +V  M+R TG    I++ +RI  GIV ++  M+V+GI E++R  R
Sbjct: 413 SLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIR 472

Query: 444 DLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSL 503
            +  G+ + + P+ V  L P+  L GL EAF  I  +E   +  PE +R++A ++F LS 
Sbjct: 473 SINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSF 532

Query: 504 SLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFYVVAVIGTLNFFYFRFF 563
           + +SYLSS LV ++ K S   D  +  W+  N LN  +L+YF+Y++AV+G +N  YF + 
Sbjct: 533 AGSSYLSSFLVTVVHKFSGGHD--RPDWLNKN-LNAGKLDYFYYLIAVLGVVNLVYFWYC 592

Query: 564 AAGFLPKGDVDDHDQTHERQLEDEE 582
           A G+  K  +   D   ++  +D E
Sbjct: 593 ARGYRYKVGLPIEDFEEDKSSDDVE 612

BLAST of MS013992 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 409.5 bits (1051), Expect = 6.6e-113
Identity = 234/574 (40.77%), Postives = 352/574 (61.32%), Query Frame = 0

Query: 16  IAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWGGT 75
           I P K  +GGWRA+ FIL NET EKL S+ + AN +LYL  +++++ V + NV  +W G 
Sbjct: 9   ILPEK-KLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGL 68

Query: 76  TNFAPIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPELRPPSCSHSQA-KCPQ 135
           TNFAP+ GA ++DAY+GRF T+ + S+ S LG+  +TL A LP+L PP C++    +C  
Sbjct: 69  TNFAPLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDD 128

Query: 136 PQLWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKLESFLNWWYFLFSIA 195
           P   QL  L+LGLG L IG+GGIRPC+I FG DQFD  T +G   + SF NW+Y   ++ 
Sbjct: 129 PNKLQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMV 188

Query: 196 LIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKTPQGSVFSDMAKVII 255
           LI + TVVVY+QT +SWV GF+IPT+    ++++F +G   Y+   P+GSVFS +A+VI+
Sbjct: 189 LIFSHTVVVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIV 248

Query: 256 ATCRKS--KLSKPNNQNNLHDPPPTIPD-STKLPHTNRFLVFDKAATVVDPAAELDEHGN 315
           A  +K   K+S  ++    +  PP  P   +KLP T++F   DKAA ++D   +L   G 
Sbjct: 249 AARKKRDLKISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILD--GDLTSEGV 308

Query: 316 SVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQMDRFLGRHFEI 375
             + WRLCS+Q+VE++KC+I +VPVW AGI   +AM    +F V QA +MDR +G HFEI
Sbjct: 309 PANKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEI 368

Query: 376 PPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVMSVVCMVVSGIT 435
           P A + +   I + IW+ +YE  +     +  +  R+++ +R+ IGIV +++ M  +G  
Sbjct: 369 PAASITVISYITIGIWVPIYEHLLVPFLWRM-RKFRVTLLQRMGIGIVFAILSMFTAGFV 428

Query: 436 EKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAI 495
           E +RR  A    + +  + V  L     L GL E+F  I ++E   +  PE +R++A ++
Sbjct: 429 EGVRRTRA----TEMTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSL 488

Query: 496 FFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFYVVAVIGTLNFF 555
           F LS + A+YLSS+LV  + K+S T D     W+   DL+  +L+YF+Y++AV+G +N  
Sbjct: 489 FPLSFAAANYLSSLLVTTVHKVSGTKD--HPDWL-NKDLDRGKLDYFYYLIAVLGVVNLV 548

Query: 556 YFRFFAAGFLPKGDVDDHDQTHERQLEDEETGRR 586
           YF + A  +  K      D   E+ L D E  +R
Sbjct: 549 YFWYCAHRYQYKAGSQIEDFNEEKSLLDIEPNQR 570

BLAST of MS013992 vs. ExPASy Swiss-Prot
Match: Q944G5 (Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana OX=3702 GN=NPF2.10 PE=1 SV=3)

HSP 1 Score: 372.9 bits (956), Expect = 6.8e-102
Identity = 211/583 (36.19%), Postives = 343/583 (58.83%), Query Frame = 0

Query: 20  KAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWGGTTNFA 79
           K    GW+ + FI+ NETFEKL  +  ++NL++YL +++NL +  +A +IN + GT NF 
Sbjct: 59  KIVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIINAFSGTINFG 118

Query: 80  PIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPELRPPSCSHSQAKCPQPQLWQ 139
               AFL D Y GR+ TL    IA FLG  V+ L A +P L P +C  ++  C  P + Q
Sbjct: 119 TFIAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACG-NKISCEGPSVGQ 178

Query: 140 LAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKLESFLNWWYFLFSIALIVAL 199
           + FL +GLG LV+GAGGIRPCN++FGADQF+  +  G+  + SF NW++F F+ A I++L
Sbjct: 179 ILFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKKGINSFFNWYFFTFTFAQIISL 238

Query: 200 TVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKTPQGSVFSDMAKVIIATCRK 259
           T VVYIQ+N+SW  G  IP A   L+ +IF  G   Y++    GS  + +A+VI A  +K
Sbjct: 239 TAVVYIQSNVSWTIGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLAGIARVIAAAIKK 298

Query: 260 SKLSKPNNQN--NLHDPPPTIPDSTKLPHTNRFLVFDKAATVVDPAAELDEHGNSVSHWR 319
             L KP  Q   NL++  P+   +T L +T++F   DKAA ++ P  +L+  G +   W+
Sbjct: 299 RGL-KPVKQPWVNLYNHIPSNYANTTLKYTDQFRFLDKAA-IMTPEEKLNSDGTASDPWK 358

Query: 320 LCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQMDRFLGR-HFEIPPAWM 379
           LC++QQVE++KC++ ++P+W A    ++A+    ++ V QA+Q DR LG   F IP A  
Sbjct: 359 LCTLQQVEEVKCIVRVIPIWFASTIYYLAITIQMTYPVFQALQSDRRLGSGGFRIPAATY 418

Query: 380 NLTPMIALSIWIFVYEK-YVAMMKRKTGQNKRISMQRRIEIGIVMSVVCMVVSGITEKLR 439
            +  M  ++++I  Y++  V  ++R TG    IS+ +RI  G   +++ ++VSG  E+ R
Sbjct: 419 VVFLMTGMTVFIIFYDRVLVPSLRRVTGLETGISLLQRIGAGFTFAIMSLLVSGFIEERR 478

Query: 440 RDLAVGNGSF--------VAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHLPESLRTV 499
           R+ A+   +         ++ +    L+P+  L+G+ EAFAAI  ME      PE++++ 
Sbjct: 479 RNFALTKPTLGMAPRTGEISSMSALWLIPQLTLAGIAEAFAAIGQMEFYYKQFPENMKSF 538

Query: 500 AGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFYVVAVIGT 559
           AG+IF++   ++SYL+S L+  + +  TT       W+   DLN  +L+YF++++  +  
Sbjct: 539 AGSIFYVGAGVSSYLASFLISTVHR--TTAHSPSGNWL-AEDLNKAKLDYFYFMLTGLMV 598

Query: 560 LNFFYFRFFAAGFLPKGDVDD--------HDQTHERQLEDEET 583
           +N  YF   A  +  KG  D+         ++T ++QL+D+ +
Sbjct: 599 VNMAYFLLMARWYRYKGGNDEDITEIETNEEETKQQQLQDKNS 635

BLAST of MS013992 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 2.6e-101
Identity = 214/568 (37.68%), Postives = 337/568 (59.33%), Query Frame = 0

Query: 20  KAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWGGTTNFA 79
           K    GW+ + FI+ NETFEKL  +  ++NL++YL  ++NL ++ +A +IN + GT NF 
Sbjct: 41  KVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFG 100

Query: 80  PIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPELRPPSC-SHSQAKCPQPQLW 139
               AFL D Y GR+ TL    IA FLG  V+ L A +P+L P +C + + + C  P   
Sbjct: 101 TFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPSGG 160

Query: 140 QLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKLESFLNWWYFLFSIALIVA 199
           Q+AFL +GLG LV+GAGGIRPCN++FGADQF+  +  G+  ++SF NW++F F+ A I++
Sbjct: 161 QIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILS 220

Query: 200 LTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKTPQGSVFSDMAKVIIATCR 259
           LT+VVY+Q+N+SW  G  IP     L+ LIF  G   Y++    GS  + +A+VI    +
Sbjct: 221 LTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIK 280

Query: 260 KSKLSKPNNQN--NLHDPPPTIPDSTKLPHTNRFLVFDKAATVVDPAAELDEHGNSVSHW 319
           K  L KP  Q   NL++  P    ++KL +T++F   DKAA ++ P  +L   G     W
Sbjct: 281 KRGL-KPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPADPW 340

Query: 320 RLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQMDRFLGR-HFEIPPAW 379
           +LC++QQVE++KC++ ++P+W A    ++ + Q  ++ V QA+Q DR LG   F IP A 
Sbjct: 341 KLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAAT 400

Query: 380 MNLTPMIALSIWIFVYEK-YVAMMKRKTGQNKRISMQRRIEIGIVMSVVCMVVSGITEKL 439
             +  M  ++++I VY++  V  M+R TG +  I++ +RI  GI  +   +VV+G  E+ 
Sbjct: 401 YVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEER 460

Query: 440 RRDLAVGNGSF--------VAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHLPESLRT 499
           RR  A+   +         ++ +    L+P+ +L+G+ EAFAAI  ME      PE++R+
Sbjct: 461 RRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMRS 520

Query: 500 VAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFYVVAVIG 559
            AG+IF++   ++SYL S L+  + +  TT +     W+   DLN  RL+ F++++A I 
Sbjct: 521 FAGSIFYVGGGVSSYLGSFLIATVHR--TTQNSSGGNWL-AEDLNKGRLDLFYFMIAGIL 580

Query: 560 TLNFFYFRFFAAGFLPKGDVDDHDQTHE 575
            +NF YF   +  +  KG  DD   T+E
Sbjct: 581 AVNFAYFLVMSRWYRYKGS-DDEVTTYE 602

BLAST of MS013992 vs. ExPASy TrEMBL
Match: A0A6J1DBD9 (protein NRT1/ PTR FAMILY 2.8-like OS=Momordica charantia OX=3673 GN=LOC111019435 PE=3 SV=1)

HSP 1 Score: 1139.4 bits (2946), Expect = 0.0e+00
Identity = 577/585 (98.63%), Postives = 579/585 (98.97%), Query Frame = 0

Query: 1   MENDAIDHSSLDPPPIAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60
           MENDAIDHSSLDPPP+APSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL
Sbjct: 1   MENDAIDHSSLDPPPVAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60

Query: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPEL 120
           DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASF GMGVLTLGAGLPEL
Sbjct: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFFGMGVLTLGAGLPEL 120

Query: 121 RPPSCSHSQAKCPQPQLWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180
           RPPSCSHSQAKCPQPQ WQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL
Sbjct: 121 RPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180

Query: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240
           ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT
Sbjct: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240

Query: 241 PQGSVFSDMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300
           PQGSVF+DMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV
Sbjct: 241 PQGSVFADMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300

Query: 301 DPAAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360
           DP AELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ
Sbjct: 301 DPVAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360

Query: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420
           MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM
Sbjct: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420

Query: 421 SVVCMVVSGITEKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHL 480
           SVVCMVVSGITEKLRRDLAVGNGSFVA LHVTAL PEFALSGLTEAFAAIAVMELLTTHL
Sbjct: 421 SVVCMVVSGITEKLRRDLAVGNGSFVARLHVTALXPEFALSGLTEAFAAIAVMELLTTHL 480

Query: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540
           PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY
Sbjct: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540

Query: 541 VVAVIGTLNFFYFRFFAAGFLPKGDVDDHDQTHERQLEDEETGRR 586
           VVAVIGTLNFFYFRFFAAGFLPK DVDDHDQTHERQLEDEETGRR
Sbjct: 541 VVAVIGTLNFFYFRFFAAGFLPKSDVDDHDQTHERQLEDEETGRR 585

BLAST of MS013992 vs. ExPASy TrEMBL
Match: A0A6J1F0P7 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111438173 PE=3 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 9.8e-245
Identity = 437/585 (74.70%), Postives = 498/585 (85.13%), Query Frame = 0

Query: 1   MENDAIDHSSLDPPPIAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60
           M+N  +  SSLDP P    K  +GGWRAVRFILANETFEKLASMSL+ANLILYLHTMYNL
Sbjct: 1   MDNGTL-RSSLDPSP----KPQLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNL 60

Query: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPEL 120
           DNV SA V  IWGGTTNF+P+AGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGA LPE+
Sbjct: 61  DNVDSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEM 120

Query: 121 RPPSCSHSQAKCPQPQLWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180
           RPP CS  +  C Q + WQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDT T KGRAKL
Sbjct: 121 RPPPCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKL 180

Query: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240
           ESFLNWWYFLFSIAL++ALT+VVYIQTNISW  GFAIPTA F+ SI+IFLLGR  YI + 
Sbjct: 181 ESFLNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRI 240

Query: 241 PQGSVFSDMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300
           PQGSVF+DMAKV+IAT RK ++ +P + N+LHDPP    DS+KLPHTNRFLVFDKAA V+
Sbjct: 241 PQGSVFADMAKVLIATYRKRQIPRP-SPNHLHDPP---MDSSKLPHTNRFLVFDKAAMVI 300

Query: 301 DPAAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360
           D + ELDE G S + W+LC+V QVEQLKCV+GIVP+W AGISCFI+MQQMGSFG+LQAIQ
Sbjct: 301 DSSMELDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQ 360

Query: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420
           M+RFLG HFEIPPAWM+L PMIALS+WI++YEKYV  M+RKT  NKR SM+ RIEIGIVM
Sbjct: 361 MNRFLGPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVM 420

Query: 421 SVVCMVVSGITEKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHL 480
           SV+CM+V+GI EK+RRD A+ N +FV+PLHV+ L+PEFALSGLTEAFAAI+VMELLTT L
Sbjct: 421 SVLCMIVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQL 480

Query: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540
           PESLRTVAGAIFFLSLSLASYLSSVL+GI++++S T D  KT+W+ GNDLN N L+YFFY
Sbjct: 481 PESLRTVAGAIFFLSLSLASYLSSVLMGIVNRVSKTGD-SKTSWLEGNDLNKNHLDYFFY 540

Query: 541 VVAVIGTLNFFYFRFFAAGFLPKGDVDDHDQTHERQLEDEETGRR 586
           VV VI  LNFFYFRF    FLP+GDVD   +  ER+ EDEE+G R
Sbjct: 541 VVGVIAALNFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGFR 575

BLAST of MS013992 vs. ExPASy TrEMBL
Match: A0A1S4DX14 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucumis melo OX=3656 GN=LOC103491761 PE=3 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 1.9e-200
Identity = 350/478 (73.22%), Postives = 402/478 (84.10%), Query Frame = 0

Query: 108 MGVLTLGAGLPELRPPSCSHSQAKCPQPQLWQLAFLYLGLGLLVIGAGGIRPCNISFGAD 167
           MGVLTLGAGLP++RPP C   ++ CPQP  WQL+FLYLGLGL+VIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLSFLYLGLGLIVIGAGGIRPCNISFGAD 60

Query: 168 QFDTTTYKGRAKLESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSIL 227
           QFDT T KGRAKLESFLNWWYFLFS+AL++ALT+VVY+QTN+SW  GF IPTACFV SI 
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYFLFSVALVIALTIVVYVQTNVSWTLGFGIPTACFVFSIS 120

Query: 228 IFLLGRGTYIRKTPQGSVFSDMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHT 287
           IFLLG   YI K PQGSVF+D+AKV++ATCRK ++ KP   + LH+PP    +S+KL HT
Sbjct: 121 IFLLGHRYYICKAPQGSVFADIAKVVVATCRKRQIPKPTQFDQLHNPP---MNSSKLAHT 180

Query: 288 NRFLVFDKAATVVDPAAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAM 347
           NRFL+FDKAATVVD + ELDE G S + WRLCSV QVE+ KCV+GI+PVW+AGI CF++M
Sbjct: 181 NRFLIFDKAATVVDSSVELDEDGKSKNEWRLCSVHQVEEFKCVVGIIPVWLAGIPCFMSM 240

Query: 348 QQMGSFGVLQAIQMDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKR 407
           QQMGSFG+LQAIQM+RF+G HF+IPPAWM+LTPMI LSIWI++YEKYV  MK+KT  NKR
Sbjct: 241 QQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMITLSIWIYIYEKYVEHMKKKTRSNKR 300

Query: 408 ISMQRRIEIGIVMSVVCMVVSGITEKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAF 467
            SM+ RIEIGIVMSV+CMVV+GI EKLRRD AV N SF++PLHV  L+PEFALSGLTEAF
Sbjct: 301 FSMKTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSFISPLHVWVLIPEFALSGLTEAF 360

Query: 468 AAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGG 527
           AAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVL GI+SK+       +  W+GG
Sbjct: 361 AAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLTGIVSKV-------EREWLGG 420

Query: 528 NDLNHNRLEYFFYVVAVIGTLNFFYFRFFAAGFLPKGDVDDHDQTHERQLEDEETGRR 586
           NDLN NRL+YFFYVV VI  LNFFYFRFFA  FLPK DVD   + H+RQLED E GRR
Sbjct: 421 NDLNKNRLDYFFYVVGVIAALNFFYFRFFAGAFLPKPDVDPRQKAHQRQLEDAELGRR 468

BLAST of MS013992 vs. ExPASy TrEMBL
Match: A0A6J1HNI7 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita maxima OX=3661 GN=LOC111466257 PE=3 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 7.6e-197
Identity = 352/478 (73.64%), Postives = 406/478 (84.94%), Query Frame = 0

Query: 108 MGVLTLGAGLPELRPPSCSHSQAKCPQPQLWQLAFLYLGLGLLVIGAGGIRPCNISFGAD 167
           MGVLTLGA LPE+RPP CS  +  C Q + WQLAFLYLGLGLLVIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAALPEMRPPPCSSGKTDCIQSKPWQLAFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 168 QFDTTTYKGRAKLESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSIL 227
           QFDT T KGRAKLESFLNWWYFLFSIAL++ALT+VVYIQTNISW  GFAIPTA F+ SI+
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIV 120

Query: 228 IFLLGRGTYIRKTPQGSVFSDMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHT 287
           IFLLGR  YI + PQGSVF+DMAKV+IAT RK ++ +P + N+LHDPP    DS+KLPHT
Sbjct: 121 IFLLGRHLYICRIPQGSVFADMAKVLIATYRKRQIPRP-SPNHLHDPP---MDSSKLPHT 180

Query: 288 NRFLVFDKAATVVDPAAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAM 347
           NRFLVFDKAA V D + ELDE G S + W+LC+V QVEQLKCV+GIVP+W AGISCFI+M
Sbjct: 181 NRFLVFDKAAMVTDSSMELDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISM 240

Query: 348 QQMGSFGVLQAIQMDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKR 407
           QQMGSFG+LQAIQM+RFLG HFEIPPAWM+L PMIALS+WI++YEKYV  MKRKT  NKR
Sbjct: 241 QQMGSFGILQAIQMNRFLGPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMKRKTHHNKR 300

Query: 408 ISMQRRIEIGIVMSVVCMVVSGITEKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAF 467
            SM+ RIEIGI+MSV+CMVV+GI EK+RRD A+ N +FV+PLHV+ L+PEFALSGLTEAF
Sbjct: 301 FSMKMRIEIGIMMSVLCMVVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAF 360

Query: 468 AAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGG 527
           AAI+VMELLTT LPESLRTVAGAIFFLSLSLASYLSSVL+GI++++S T D  KT+W+ G
Sbjct: 361 AAISVMELLTTQLPESLRTVAGAIFFLSLSLASYLSSVLMGIVNRVSKTED-SKTSWLEG 420

Query: 528 NDLNHNRLEYFFYVVAVIGTLNFFYFRFFAAGFLPKGDVDDHDQTHERQLEDEETGRR 586
           NDLN N L+YFFYVV VI  LNFFYFRF    FLP+GDVD   + +ER+ EDEE+G R
Sbjct: 421 NDLNKNHLDYFFYVVGVIAALNFFYFRFVGTRFLPEGDVDPRVKANERRSEDEESGFR 473

BLAST of MS013992 vs. ExPASy TrEMBL
Match: A0A6J1IS88 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita maxima OX=3661 GN=LOC111478233 PE=3 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 3.5e-194
Identity = 342/459 (74.51%), Postives = 391/459 (85.19%), Query Frame = 0

Query: 108 MGVLTLGAGLPELRPPSCSHSQAKCPQPQLWQLAFLYLGLGLLVIGAGGIRPCNISFGAD 167
           MGVLTLGAG PE+RPP C H ++ CPQP  WQL FLYLGLGLLVIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 168 QFDTTTYKGRAKLESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSIL 227
           QFDT T KGRAKLESFLNWWY LFS+AL++ALT+VVY+QT+ISW  GFAIPTACFV SIL
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL 120

Query: 228 IFLLGRGTYIRKTPQGSVFSDMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHT 287
           IFLLGR  YI KTPQGSVF+DMAKV++ATCRK  + K    ++L+DPP    DS+KL HT
Sbjct: 121 IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLYDPP---IDSSKLAHT 180

Query: 288 NRFLVFDKAATVVDPAAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAM 347
           +RFLVFDKAATV D + ELDE G S + W+LCSV QVEQLKCV+GIVP+W AGI CFI+M
Sbjct: 181 DRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISM 240

Query: 348 QQMGSFGVLQAIQMDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKR 407
           QQMGSFG+LQAIQM+RF+G HF+IPPAWM+LTPMIALSIWI++YEKYV  MK+KT +NKR
Sbjct: 241 QQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKR 300

Query: 408 ISMQRRIEIGIVMSVVCMVVSGITEKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAF 467
            SM+ RIEIGIVMSV+CMVV+GI E +RR  AV N  FV+PLHV  L+PEFALSGLTEAF
Sbjct: 301 FSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAF 360

Query: 468 AAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGG 527
           AAIAVMELLTTHLPESLRTVAGAIFFLSLSL SY+SSVL+ I+SKI+  ++G K+ W+GG
Sbjct: 361 AAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKIN-RVEGGKSEWLGG 420

Query: 528 NDLNHNRLEYFFYVVAVIGTLNFFYFRFFAAGFLPKGDV 567
           NDLN NRL+YFFYVV VI   NFFYFRFFA  FLPK +V
Sbjct: 421 NDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 455

BLAST of MS013992 vs. TAIR 10
Match: AT5G28470.1 (Major facilitator superfamily protein )

HSP 1 Score: 575.1 bits (1481), Expect = 6.4e-164
Identity = 301/558 (53.94%), Postives = 395/558 (70.79%), Query Frame = 0

Query: 6   IDHSSLDPPPIAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVAS 65
           +D  S  P   A  K   GGWRA+++I+ANE+FEKLASMSL+ NL +YL T YNL  V  
Sbjct: 1   MDVESSSPSSHALIKKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFL 60

Query: 66  ANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPELRPPSC 125
            NVINIW G+ N   +AGAF++DAYLGRF+TLL GSIASF+GMG+  L A LP LRP +C
Sbjct: 61  VNVINIWFGSCNILTLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDAC 120

Query: 126 SHSQAKCPQPQLWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKLESFLN 185
                   QP  WQL  L+ GLGLL IGAGG+RPCNI+FGADQFDT+T KG+A LE+F N
Sbjct: 121 IDPSNCSNQPAKWQLGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFN 180

Query: 186 WWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKTPQGSV 245
           WWYF F++AL++ALT VVYIQTNISWV GF IPTAC  LSI  F++G+ TYI    +GSV
Sbjct: 181 WWYFSFTVALVIALTGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSV 240

Query: 246 FSDMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPH-TNRFLVFDKAATVVDPAA 305
           F+D+ KV+ A C+K K+ KP +    +  P      T L    +R   FDKA+ V +P  
Sbjct: 241 FADIVKVVTAACKKRKV-KPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKASIVTNP-N 300

Query: 306 ELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQMDRF 365
           EL+E GN+   WRLCSVQQV+ LKCV  I+PVWV GI+CFI   Q   +G+LQA+QMD+ 
Sbjct: 301 ELNEDGNAKYKWRLCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKT 360

Query: 366 LGRH-FEIPPAWMNLTPMIALSIWIFVYE-KYVAMMKRKTGQNKRISMQRRIEIGIVMSV 425
            G H F++P  WMNL  MI L+IWI +YE   + ++K+ TG+ KR++++ RIE  IVM +
Sbjct: 361 FGPHNFQVPAGWMNLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGI 420

Query: 426 VCMVVSGITEKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHLPE 485
           +CM+V+G  EK RR  A+ NGSFV+P+ +  L+P+FAL+GLTEAF+A+A+ME LT  +PE
Sbjct: 421 ICMIVAGFQEKKRRASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPE 480

Query: 486 SLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFYVV 545
            +R VAGAIFFLS S+ASY+ ++L+ +I  + T  +GK  +W+G  DLN NRLE +F+++
Sbjct: 481 HMRAVAGAIFFLSSSIASYICTLLINVIDAV-TRKEGK--SWLGDKDLNKNRLENYFFII 540

Query: 546 AVIGTLNFFYFRFFAAGF 561
           A I   N  YFR FA+ +
Sbjct: 541 AGIQVANLLYFRLFASRY 551

BLAST of MS013992 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 429.9 bits (1104), Expect = 3.3e-120
Identity = 236/565 (41.77%), Postives = 349/565 (61.77%), Query Frame = 0

Query: 24  GGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWGGTTNFAPIAG 83
           GGWRAV FIL NET E+L S+ L+AN ++YL  +++L+ V +ANVINIW G TN  P+ G
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 84  AFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPELRPPSC-SHSQAKCPQPQLWQLAF 143
           A+++D Y+GRF T+ F S A+ LG+  +TL A  P+L P SC S     C  P   Q+  
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGV 172

Query: 144 LYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKLESFLNWWYFLFSIALIVALTVV 203
           L LGL  L +G+GGIRPC+I FG DQFD  T +G   + SF NW+Y  F++ LI+  TVV
Sbjct: 173 LLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVV 232

Query: 204 VYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKTPQGSVFSDMAKVIIATCRKSKL 263
           VYIQ  +SW+ GF+IPT    L++++F  G   Y+   P+GS+FS +A+VI+A  +K KL
Sbjct: 233 VYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKL 292

Query: 264 SKPNNQN---NLHDPPPTIPDSTKLPHTNRFLVFDKAATVVDPAAELDEHGNSVSHWRLC 323
             P   +     +DP       +KL  +N+F   DKAA V++   +L   G     WRLC
Sbjct: 293 KLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIE--GDLTPEGPPADKWRLC 352

Query: 324 SVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQMDRFLGRHFEIPPAWMNLT 383
           SVQ+VE++KC+I IVP+W AGI    AM   G+F V QA++MDR LG  FEIP   +++ 
Sbjct: 353 SVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVI 412

Query: 384 PMIALSIWIFVYEK-YVAMMKRKTGQNKRISMQRRIEIGIVMSVVCMVVSGITEKLR--R 443
            ++ + I++  Y++ +V  M+R TG    I++ +RI  GIV ++  M+V+GI E++R  R
Sbjct: 413 SLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIR 472

Query: 444 DLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSL 503
            +  G+ + + P+ V  L P+  L GL EAF  I  +E   +  PE +R++A ++F LS 
Sbjct: 473 SINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSF 532

Query: 504 SLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFYVVAVIGTLNFFYFRFF 563
           + +SYLSS LV ++ K S   D  +  W+  N LN  +L+YF+Y++AV+G +N  YF + 
Sbjct: 533 AGSSYLSSFLVTVVHKFSGGHD--RPDWLNKN-LNAGKLDYFYYLIAVLGVVNLVYFWYC 592

Query: 564 AAGFLPKGDVDDHDQTHERQLEDEE 582
           A G+  K  +   D   ++  +D E
Sbjct: 593 ARGYRYKVGLPIEDFEEDKSSDDVE 612

BLAST of MS013992 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 409.5 bits (1051), Expect = 4.7e-114
Identity = 234/574 (40.77%), Postives = 352/574 (61.32%), Query Frame = 0

Query: 16  IAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWGGT 75
           I P K  +GGWRA+ FIL NET EKL S+ + AN +LYL  +++++ V + NV  +W G 
Sbjct: 9   ILPEK-KLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGL 68

Query: 76  TNFAPIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPELRPPSCSHSQA-KCPQ 135
           TNFAP+ GA ++DAY+GRF T+ + S+ S LG+  +TL A LP+L PP C++    +C  
Sbjct: 69  TNFAPLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDD 128

Query: 136 PQLWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKLESFLNWWYFLFSIA 195
           P   QL  L+LGLG L IG+GGIRPC+I FG DQFD  T +G   + SF NW+Y   ++ 
Sbjct: 129 PNKLQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMV 188

Query: 196 LIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKTPQGSVFSDMAKVII 255
           LI + TVVVY+QT +SWV GF+IPT+    ++++F +G   Y+   P+GSVFS +A+VI+
Sbjct: 189 LIFSHTVVVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIV 248

Query: 256 ATCRKS--KLSKPNNQNNLHDPPPTIPD-STKLPHTNRFLVFDKAATVVDPAAELDEHGN 315
           A  +K   K+S  ++    +  PP  P   +KLP T++F   DKAA ++D   +L   G 
Sbjct: 249 AARKKRDLKISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILD--GDLTSEGV 308

Query: 316 SVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQMDRFLGRHFEI 375
             + WRLCS+Q+VE++KC+I +VPVW AGI   +AM    +F V QA +MDR +G HFEI
Sbjct: 309 PANKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEI 368

Query: 376 PPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVMSVVCMVVSGIT 435
           P A + +   I + IW+ +YE  +     +  +  R+++ +R+ IGIV +++ M  +G  
Sbjct: 369 PAASITVISYITIGIWVPIYEHLLVPFLWRM-RKFRVTLLQRMGIGIVFAILSMFTAGFV 428

Query: 436 EKLRRDLAVGNGSFVAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAI 495
           E +RR  A    + +  + V  L     L GL E+F  I ++E   +  PE +R++A ++
Sbjct: 429 EGVRRTRA----TEMTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSL 488

Query: 496 FFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFYVVAVIGTLNFF 555
           F LS + A+YLSS+LV  + K+S T D     W+   DL+  +L+YF+Y++AV+G +N  
Sbjct: 489 FPLSFAAANYLSSLLVTTVHKVSGTKD--HPDWL-NKDLDRGKLDYFYYLIAVLGVVNLV 548

Query: 556 YFRFFAAGFLPKGDVDDHDQTHERQLEDEETGRR 586
           YF + A  +  K      D   E+ L D E  +R
Sbjct: 549 YFWYCAHRYQYKAGSQIEDFNEEKSLLDIEPNQR 570

BLAST of MS013992 vs. TAIR 10
Match: AT3G47960.1 (Major facilitator superfamily protein )

HSP 1 Score: 372.9 bits (956), Expect = 4.9e-103
Identity = 211/583 (36.19%), Postives = 343/583 (58.83%), Query Frame = 0

Query: 20  KAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWGGTTNFA 79
           K    GW+ + FI+ NETFEKL  +  ++NL++YL +++NL +  +A +IN + GT NF 
Sbjct: 59  KIVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIINAFSGTINFG 118

Query: 80  PIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPELRPPSCSHSQAKCPQPQLWQ 139
               AFL D Y GR+ TL    IA FLG  V+ L A +P L P +C  ++  C  P + Q
Sbjct: 119 TFIAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACG-NKISCEGPSVGQ 178

Query: 140 LAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKLESFLNWWYFLFSIALIVAL 199
           + FL +GLG LV+GAGGIRPCN++FGADQF+  +  G+  + SF NW++F F+ A I++L
Sbjct: 179 ILFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKKGINSFFNWYFFTFTFAQIISL 238

Query: 200 TVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKTPQGSVFSDMAKVIIATCRK 259
           T VVYIQ+N+SW  G  IP A   L+ +IF  G   Y++    GS  + +A+VI A  +K
Sbjct: 239 TAVVYIQSNVSWTIGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLAGIARVIAAAIKK 298

Query: 260 SKLSKPNNQN--NLHDPPPTIPDSTKLPHTNRFLVFDKAATVVDPAAELDEHGNSVSHWR 319
             L KP  Q   NL++  P+   +T L +T++F   DKAA ++ P  +L+  G +   W+
Sbjct: 299 RGL-KPVKQPWVNLYNHIPSNYANTTLKYTDQFRFLDKAA-IMTPEEKLNSDGTASDPWK 358

Query: 320 LCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQMDRFLGR-HFEIPPAWM 379
           LC++QQVE++KC++ ++P+W A    ++A+    ++ V QA+Q DR LG   F IP A  
Sbjct: 359 LCTLQQVEEVKCIVRVIPIWFASTIYYLAITIQMTYPVFQALQSDRRLGSGGFRIPAATY 418

Query: 380 NLTPMIALSIWIFVYEK-YVAMMKRKTGQNKRISMQRRIEIGIVMSVVCMVVSGITEKLR 439
            +  M  ++++I  Y++  V  ++R TG    IS+ +RI  G   +++ ++VSG  E+ R
Sbjct: 419 VVFLMTGMTVFIIFYDRVLVPSLRRVTGLETGISLLQRIGAGFTFAIMSLLVSGFIEERR 478

Query: 440 RDLAVGNGSF--------VAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHLPESLRTV 499
           R+ A+   +         ++ +    L+P+  L+G+ EAFAAI  ME      PE++++ 
Sbjct: 479 RNFALTKPTLGMAPRTGEISSMSALWLIPQLTLAGIAEAFAAIGQMEFYYKQFPENMKSF 538

Query: 500 AGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFYVVAVIGT 559
           AG+IF++   ++SYL+S L+  + +  TT       W+   DLN  +L+YF++++  +  
Sbjct: 539 AGSIFYVGAGVSSYLASFLISTVHR--TTAHSPSGNWL-AEDLNKAKLDYFYFMLTGLMV 598

Query: 560 LNFFYFRFFAAGFLPKGDVDD--------HDQTHERQLEDEET 583
           +N  YF   A  +  KG  D+         ++T ++QL+D+ +
Sbjct: 599 VNMAYFLLMARWYRYKGGNDEDITEIETNEEETKQQQLQDKNS 635

BLAST of MS013992 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 370.9 bits (951), Expect = 1.8e-102
Identity = 214/568 (37.68%), Postives = 337/568 (59.33%), Query Frame = 0

Query: 20  KAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWGGTTNFA 79
           K    GW+ + FI+ NETFEKL  +  ++NL++YL  ++NL ++ +A +IN + GT NF 
Sbjct: 41  KVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFG 100

Query: 80  PIAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPELRPPSC-SHSQAKCPQPQLW 139
               AFL D Y GR+ TL    IA FLG  V+ L A +P+L P +C + + + C  P   
Sbjct: 101 TFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPSGG 160

Query: 140 QLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKLESFLNWWYFLFSIALIVA 199
           Q+AFL +GLG LV+GAGGIRPCN++FGADQF+  +  G+  ++SF NW++F F+ A I++
Sbjct: 161 QIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILS 220

Query: 200 LTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKTPQGSVFSDMAKVIIATCR 259
           LT+VVY+Q+N+SW  G  IP     L+ LIF  G   Y++    GS  + +A+VI    +
Sbjct: 221 LTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIK 280

Query: 260 KSKLSKPNNQN--NLHDPPPTIPDSTKLPHTNRFLVFDKAATVVDPAAELDEHGNSVSHW 319
           K  L KP  Q   NL++  P    ++KL +T++F   DKAA ++ P  +L   G     W
Sbjct: 281 KRGL-KPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPADPW 340

Query: 320 RLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQMDRFLGR-HFEIPPAW 379
           +LC++QQVE++KC++ ++P+W A    ++ + Q  ++ V QA+Q DR LG   F IP A 
Sbjct: 341 KLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAAT 400

Query: 380 MNLTPMIALSIWIFVYEK-YVAMMKRKTGQNKRISMQRRIEIGIVMSVVCMVVSGITEKL 439
             +  M  ++++I VY++  V  M+R TG +  I++ +RI  GI  +   +VV+G  E+ 
Sbjct: 401 YVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEER 460

Query: 440 RRDLAVGNGSF--------VAPLHVTALVPEFALSGLTEAFAAIAVMELLTTHLPESLRT 499
           RR  A+   +         ++ +    L+P+ +L+G+ EAFAAI  ME      PE++R+
Sbjct: 461 RRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMRS 520

Query: 500 VAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFYVVAVIG 559
            AG+IF++   ++SYL S L+  + +  TT +     W+   DLN  RL+ F++++A I 
Sbjct: 521 FAGSIFYVGGGVSSYLGSFLIATVHR--TTQNSSGGNWL-AEDLNKGRLDLFYFMIAGIL 580

Query: 560 TLNFFYFRFFAAGFLPKGDVDDHDQTHE 575
            +NF YF   +  +  KG  DD   T+E
Sbjct: 581 AVNFAYFLVMSRWYRYKGS-DDEVTTYE 602