MS013916 (gene) Bitter gourd (TR) v1

BLAST of MS013916 vs. NCBI nr
Match: XP_022144001.1 (pyruvate decarboxylase 1-like [Momordica charantia])

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 577/584 (98.80%), Postives = 578/584 (98.97%), Query Frame = 0

Query: 1   NGAAAPTNKPIQIQTQSPPPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGL 60
           NGAAAPTNKPIQIQTQSPPPWTLGH+LARRLVEIGVGDIFSVPGD NLLLLDYFVAE GL
Sbjct: 23  NGAAAPTNKPIQIQTQSPPPWTLGHYLARRLVEIGVGDIFSVPGDXNLLLLDYFVAEXGL 82

Query: 61  NLVGCCSELNAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPN 120
           NLVGCCSELNAGYAADGYAR RGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPN
Sbjct: 83  NLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPN 142

Query: 121 SNDYGSKKIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPV 180
           SNDYGSKKIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEE QWEIDRAICKCL ERKPV
Sbjct: 143 SNDYGSKKIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEETQWEIDRAICKCLGERKPV 202

Query: 181 YISICCNLVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKK 240
           YISICCNLVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKK
Sbjct: 203 YISICCNLVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKK 262

Query: 241 LRLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADA 300
           LR AKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADA
Sbjct: 263 LRPAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADA 322

Query: 301 SIFAGATLDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPN 360
           SIFAGATLDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPN
Sbjct: 323 SIFAGATLDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPN 382

Query: 361 PTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLK 420
           PTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLK
Sbjct: 383 PTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLK 442

Query: 421 LPKSCGYEVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQK 480
           LPKSCGYEVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQK
Sbjct: 443 LPKSCGYEVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQK 502

Query: 481 NIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIE 540
           NIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIE
Sbjct: 503 NIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIE 562

Query: 541 TATKDRNDCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRPL 585
           TATKDRNDCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRPL
Sbjct: 563 TATKDRNDCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRPL 606

BLAST of MS013916 vs. NCBI nr
Match: XP_038881352.1 (pyruvate decarboxylase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1028.9 bits (2659), Expect = 1.7e-296
Identity = 498/582 (85.57%), Postives = 537/582 (92.27%), Query Frame = 0

Query: 1   NGAAAPTNKPIQIQTQSPPPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGL 60
           NGAAAP+  P+ I   +  P TLGH+LARRLV+IG+ DIFSVPGDSNL+L DYFVAE+GL
Sbjct: 43  NGAAAPS--PVIIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAERGL 102

Query: 61  NLVGCCSELNAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPN 120
           NLVGCC+ELNAGYAADGYAR RGVGACAVTFTVG LSLINA+AGAYSEDLPV+CIVGGPN
Sbjct: 103 NLVGCCNELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPN 162

Query: 121 SNDYGSKKIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPV 180
           SNDYGSKKI+HHTIGLPDFSQELRCFQNVTCYQAIIDN+E+AQW+IDRAI KCLEERKPV
Sbjct: 163 SNDYGSKKILHHTIGLPDFSQELRCFQNVTCYQAIIDNLEDAQWQIDRAISKCLEERKPV 222

Query: 181 YISICCNLVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKK 240
           YISICCNLVAIPHPSFS ++LIPLSL PK+SNQM LEVA EKAAQLLNSA+KPVM+ GKK
Sbjct: 223 YISICCNLVAIPHPSFSAQQLIPLSLSPKKSNQMALEVAVEKAAQLLNSAIKPVMIGGKK 282

Query: 241 LRLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADA 300
           LR AKAQ AF+ELADAC YAVAVTPSAKG+FPE HPHFIGTYWG VSTA+CGETVEIADA
Sbjct: 283 LRPAKAQEAFMELADACRYAVAVTPSAKGMFPEYHPHFIGTYWGTVSTAYCGETVEIADA 342

Query: 301 SIFAGATLDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPN 360
           SIFAGATLDELETVAFSLTYKKNKAIIVE +RVVFPNGPSFGPILMKDFL A+A+RL PN
Sbjct: 343 SIFAGATLDELETVAFSLTYKKNKAIIVEADRVVFPNGPSFGPILMKDFLSAIAKRLNPN 402

Query: 361 PTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLK 420
           PTAYENYRRIY+ ESGPLQS PGEALRVNVLFKHIQKMLSSN+ VISE GDSWF CQKLK
Sbjct: 403 PTAYENYRRIYIAESGPLQSNPGEALRVNVLFKHIQKMLSSNIVVISETGDSWFQCQKLK 462

Query: 421 LPKSCGYEVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQK 480
           LPKSCGYE+QLLYASIGWSLGATLGYAQAAP++R+VLCIGDGSFQM+PQDVSTM+ L Q 
Sbjct: 463 LPKSCGYEIQLLYASIGWSLGATLGYAQAAPEKRMVLCIGDGSFQMTPQDVSTMITLKQN 522

Query: 481 NIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIE 540
           NIIFLINNGGYTIEVEIHDGPYNVIKNWDYTA+VNAMHN+DG CWT K+HTEEELV AIE
Sbjct: 523 NIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAIVNAMHNRDGNCWTTKIHTEEELVNAIE 582

Query: 541 TATKDRNDCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPR 583
            A  D+ DCLCFIE I+HRDDTSKELLEFGSK AAIS RPPR
Sbjct: 583 KAMGDQKDCLCFIEVIIHRDDTSKELLEFGSKFAAISSRPPR 622

BLAST of MS013916 vs. NCBI nr
Match: TYK29657.1 (pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1001.9 bits (2589), Expect = 2.3e-288
Identity = 480/577 (83.19%), Postives = 524/577 (90.81%), Query Frame = 0

Query: 8   NKPIQIQTQSPPPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCS 67
           N  + I T +  P TLGH+LARRLV+IG+ DIFSVPGDSNL++ DYFVAEKGLNLVGCC+
Sbjct: 65  NGAVTIPTPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCN 124

Query: 68  ELNAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSK 127
           ELNAGYAADGYAR RGVGACAVTFTVG LSLINA+AGAYSEDLPV+CIVGGPNSNDYGSK
Sbjct: 125 ELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSK 184

Query: 128 KIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCN 187
           KI+HHTIGLPDF QELRCFQNVTCYQAIIDN+E+AQW+ID AI KCLEE KP+YISICCN
Sbjct: 185 KILHHTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCN 244

Query: 188 LVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQ 247
           LVAIPHPSFS + LIPLSL PKQSNQM LE A EKAAQLLN+A+KPVM+ GKKLR  K+Q
Sbjct: 245 LVAIPHPSFSAQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQ 304

Query: 248 AAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGAT 307
             F+ELA+ACGY VAVTPSAKG+FPENHPHFIG YWG VS+A+CGETVEIADASIF GAT
Sbjct: 305 EPFIELANACGYPVAVTPSAKGMFPENHPHFIGIYWGTVSSAYCGETVEIADASIFVGAT 364

Query: 308 LDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENY 367
           LDELETVAF+LTYKKNKAI+VE +RVVFPNGPSFGPILMKDFL ALA+RLKPN TAYENY
Sbjct: 365 LDELETVAFTLTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENY 424

Query: 368 RRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGY 427
           RRIY+ ESGPL+S PGEALRVN+LFKHIQKMLSSNM VISEAGDSWFHCQ+LKLPKSCGY
Sbjct: 425 RRIYIAESGPLESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGY 484

Query: 428 EVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLIN 487
           EVQLLYASIGWSLGATLGYAQAAP++RVVLCIGDGSFQM+PQDVSTM+ L Q NIIFLIN
Sbjct: 485 EVQLLYASIGWSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLIN 544

Query: 488 NGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRN 547
           NGGYTIEVEIHDGPYNVIKNWDYTA+V AMHN DG CWT KVHTEEEL+ AIE   ++R 
Sbjct: 545 NGGYTIEVEIHDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERK 604

Query: 548 DCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRPL 585
           D LCFIE I+HRDDTS+ELLEFGSK AA+S RPPRPL
Sbjct: 605 DYLCFIEVIVHRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of MS013916 vs. NCBI nr
Match: XP_008464213.1 (PREDICTED: pyruvate decarboxylase 1-like [Cucumis melo] >KAA0037741.1 pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1000.0 bits (2584), Expect = 8.7e-288
Identity = 479/577 (83.02%), Postives = 524/577 (90.81%), Query Frame = 0

Query: 8   NKPIQIQTQSPPPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCS 67
           N  + I T +  P TLGH+LARRLV+IG+ DIFSVPGDSNL++ DYFVAEKGLNLVGCC+
Sbjct: 65  NGAVTIPTPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCN 124

Query: 68  ELNAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSK 127
           ELNAGYAADGYAR RGVGACAVTFTVG LSLINA+AGAYSEDLPV+CIVGGPNSNDYGSK
Sbjct: 125 ELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSK 184

Query: 128 KIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCN 187
           KI+HHTIGLPDF QELRCFQNVTCYQAIIDN+E+AQW+ID AI KCLEE KP+YISICCN
Sbjct: 185 KILHHTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCN 244

Query: 188 LVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQ 247
           LVAIPHPSFS + LIPLSL PKQSNQM LE A EKAAQLLN+A+KPVM+ GKKLR  K+Q
Sbjct: 245 LVAIPHPSFSAQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQ 304

Query: 248 AAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGAT 307
             F+ELA+ACGY VAVTPSAKG+FPE+HPHFIG YWG VS+A+CGETVEIADASIF GAT
Sbjct: 305 EPFIELANACGYPVAVTPSAKGMFPESHPHFIGIYWGTVSSAYCGETVEIADASIFVGAT 364

Query: 308 LDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENY 367
           LDELETVAF+LTYKKNKAI+VE +RVVFPNGPSFGPILMKDFL ALA+RLKPN TAYENY
Sbjct: 365 LDELETVAFTLTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENY 424

Query: 368 RRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGY 427
           RRIY+ ESGPL+S PGEALRVN+LFKHIQKMLSSNM VISEAGDSWFHCQ+LKLPKSCGY
Sbjct: 425 RRIYIAESGPLESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGY 484

Query: 428 EVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLIN 487
           EVQLLYASIGWSLGATLGYAQAAP++RVVLCIGDGSFQM+PQDVSTM+ L Q NIIFLIN
Sbjct: 485 EVQLLYASIGWSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLIN 544

Query: 488 NGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRN 547
           NGGYTIEVEIHDGPYNVIKNWDYTA+V AMHN DG CWT KVHTEEEL+ AIE   ++R 
Sbjct: 545 NGGYTIEVEIHDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERK 604

Query: 548 DCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRPL 585
           D LCFIE I+HRDDTS+ELLEFGSK AA+S RPPRPL
Sbjct: 605 DYLCFIEVIVHRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of MS013916 vs. NCBI nr
Match: XP_023544035.1 (pyruvate decarboxylase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 996.9 bits (2576), Expect = 7.4e-287
Identity = 476/575 (82.78%), Postives = 528/575 (91.83%), Query Frame = 0

Query: 8   NKPIQIQTQSPPPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCS 67
           N  ++I      P TLGH+LARRLV+IGV DIFSVPGDSNL+LLDYFVAEKGLNLVGCCS
Sbjct: 50  NNGVEIVESMVTPSTLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAEKGLNLVGCCS 109

Query: 68  ELNAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSK 127
           ELNAGYAADGYAR RGVGAC VTFTVGGLSLINA+AGAYSEDLP++CIVG PNSN+YGS 
Sbjct: 110 ELNAGYAADGYARRRGVGACVVTFTVGGLSLINAIAGAYSEDLPLVCIVGAPNSNEYGSN 169

Query: 128 KIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCN 187
           KI+HHTIGLPDFSQELRCFQNVTCYQA+I+N+E+AQWEID AI KCLE+ KPVYISI CN
Sbjct: 170 KILHHTIGLPDFSQELRCFQNVTCYQAVINNLEDAQWEIDTAISKCLEQSKPVYISISCN 229

Query: 188 LVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQ 247
           L AIPHPSFSM++ +PLS+  KQ+NQMGLE+A E AAQLLN+AVKPVMV GKKLR+AKAQ
Sbjct: 230 LAAIPHPSFSMKQFLPLSISSKQTNQMGLEMAVEMAAQLLNTAVKPVMVGGKKLRVAKAQ 289

Query: 248 AAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGAT 307
           AAFVELAD C YAVAVTPSAKG+FPENH HFIGTYWG++S+AFCGETVEIADASIFAGA 
Sbjct: 290 AAFVELADVCDYAVAVTPSAKGMFPENHRHFIGTYWGSLSSAFCGETVEIADASIFAGAI 349

Query: 308 LDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENY 367
            DELETV +SL+YKKNKAIIVE +RVVFPNGPSFGPILMKDFLRALA+RLKPNPTAYENY
Sbjct: 350 FDELETVGYSLSYKKNKAIIVESDRVVFPNGPSFGPILMKDFLRALAKRLKPNPTAYENY 409

Query: 368 RRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGY 427
            RIY+P+SGP QSEPGEALRVNVLFKHIQKMLS NM VISEAGDSWFHCQKL+LP+SCG+
Sbjct: 410 TRIYIPKSGPTQSEPGEALRVNVLFKHIQKMLSGNMAVISEAGDSWFHCQKLELPRSCGF 469

Query: 428 EVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLIN 487
           E+QL+YASIGWSLGATLGYAQA+P+ RVVLCIGDGSFQM+PQD+STMLRLGQ+NIIFLIN
Sbjct: 470 EMQLVYASIGWSLGATLGYAQASPEDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLIN 529

Query: 488 NGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRN 547
           NGGYTIEVEIHDGPYNVIKNWDYT +V AMHNQ G CWT KV TEEELVKAIE AT D+N
Sbjct: 530 NGGYTIEVEIHDGPYNVIKNWDYTGLVEAMHNQTGNCWTTKVETEEELVKAIEMATGDKN 589

Query: 548 DCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPR 583
           +CLCFIEAI+HRDDTSKELL+FG+K+AA + RPP+
Sbjct: 590 ECLCFIEAIVHRDDTSKELLDFGNKLAATNSRPPK 624

BLAST of MS013916 vs. ExPASy Swiss-Prot
Match: Q9M040 (Pyruvate decarboxylase 4 OS=Arabidopsis thaliana OX=3702 GN=PDC4 PE=2 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 1.8e-235
Identity = 392/582 (67.35%), Postives = 468/582 (80.41%), Query Frame = 0

Query: 6   PTNKPIQIQTQSP----PPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLN 65
           P+N    IQ  +P       TLG HL+RRLV+ GV D+FSVPGD NL LLD+ +AE  LN
Sbjct: 22  PSNAVATIQDSAPITTTSESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELN 81

Query: 66  LVGCCSELNAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNS 125
            +GCC+ELNAGYAADGYAR RGVGAC VTFTVGGLS++NA+AGAYSE+LPV+CIVGGPNS
Sbjct: 82  NIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNS 141

Query: 126 NDYGSKKIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVY 185
           ND+G+ +I+HHTIGLPDFSQELRCFQ VTCYQA+++N+E+A  +ID+AI   L+E KPVY
Sbjct: 142 NDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALKESKPVY 201

Query: 186 ISICCNLVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKL 245
           ISI CNL A PHP+F+ +  +P  L P+ SN MGLE A E   + LN AVKPVMV G KL
Sbjct: 202 ISISCNLAATPHPTFARDP-VPFDLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGPKL 261

Query: 246 RLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADAS 305
           R+AKA  AF+ELADA GY +AV PS KGL PENHPHFIGTYWGAVST FC E VE ADA 
Sbjct: 262 RVAKASEAFLELADASGYPLAVMPSTKGLVPENHPHFIGTYWGAVSTPFCSEIVESADAY 321

Query: 306 IFAGATLDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNP 365
           IFAG   ++  +V +SL  KK KAIIV P+RVV  NGP+FG +LM DF R LA+R+K N 
Sbjct: 322 IFAGPIFNDYSSVGYSLLLKKEKAIIVHPDRVVVANGPTFGCVLMSDFFRELAKRVKRNE 381

Query: 366 TAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKL 425
           TAYENY RI+VPE  PL+ +PGE LRVN +F+HIQKMLSS   VI+E GDSWF+CQKLKL
Sbjct: 382 TAYENYERIFVPEGKPLKCKPGEPLRVNAMFQHIQKMLSSETAVIAETGDSWFNCQKLKL 441

Query: 426 PKSCGYEVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKN 485
           PK CGYE Q+ Y SIGWS+GATLGYAQA P++RV+  IGDGSFQ++ QD+STM+R GQK 
Sbjct: 442 PKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDISTMIRNGQKA 501

Query: 486 IIFLINNGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIET 545
           IIFLINNGGYTIEVEIHDGPYNVIKNW+YT +V+A+HN +GKCWT KV  EEELV+AI+T
Sbjct: 502 IIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAIKT 561

Query: 546 ATKDRNDCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRP 584
           AT ++ D LCFIE I+H+DDTSKELLE+GS+++A + RPP P
Sbjct: 562 ATTEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGRPPNP 602

BLAST of MS013916 vs. ExPASy Swiss-Prot
Match: O82647 (Pyruvate decarboxylase 1 OS=Arabidopsis thaliana OX=3702 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 2.3e-235
Identity = 388/562 (69.04%), Postives = 464/562 (82.56%), Query Frame = 0

Query: 22  TLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCSELNAGYAADGYARL 81
           TLG HLARRLV+ GV D+FSVPGD NL LLD+ +AE  LNL+GCC+ELNAGYAADGYAR 
Sbjct: 46  TLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYARS 105

Query: 82  RGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKIIHHTIGLPDFSQ 141
           RGVGAC VTFTVGGLS++NA+AGAYSE+LP++CIVGGPNSNDYG+ +I+HHTIGLPDFSQ
Sbjct: 106 RGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFSQ 165

Query: 142 ELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCNLVAIPHPSFSMERL 201
           ELRCFQ VTCYQA+++N+++A  +ID+AI   L+E KPVYIS+ CNL AIPH +FS +  
Sbjct: 166 ELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKESKPVYISVSCNLAAIPHHTFSRDP- 225

Query: 202 IPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQAAFVELADACGYAV 261
           +P SL P+ SN+MGLE A E   + LN AVKPVMV G KLR+AKA  AFVELADA GYA+
Sbjct: 226 VPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELADASGYAL 285

Query: 262 AVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLDELETVAFSLTYK 321
           A+ PSAKG  PE+HPHFIGTYWGAVST FC E VE ADA IFAG   ++  +V +SL  K
Sbjct: 286 AMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLLK 345

Query: 322 KNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRRIYVPESGPLQSE 381
           K KAI+V+P+R+   NGP+FG ILM DF R L++R+K N TAYENY RI+VPE  PL+ E
Sbjct: 346 KEKAIVVQPDRITVANGPTFGCILMSDFFRELSKRVKRNETAYENYHRIFVPEGKPLKCE 405

Query: 382 PGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEVQLLYASIGWSLG 441
             E LRVN +F+HIQKMLSS   VI+E GDSWF+CQKLKLPK CGYE Q+ Y SIGWS+G
Sbjct: 406 SREPLRVNTMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVG 465

Query: 442 ATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNGGYTIEVEIHDGP 501
           ATLGYAQA+P++RV+  IGDGSFQ++ QD+STMLR GQK IIFLINNGGYTIEVEIHDGP
Sbjct: 466 ATLGYAQASPEKRVLAFIGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEVEIHDGP 525

Query: 502 YNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDCLCFIEAILHRDD 561
           YNVIKNW+YT +V+A+HN +G CWTAKV  EEELV+AI TAT ++ DCLCFIE ILH+DD
Sbjct: 526 YNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEELVEAITTATTEKKDCLCFIEVILHKDD 585

Query: 562 TSKELLEFGSKIAAISCRPPRP 584
           TSKELLE+GS+++A + RPP P
Sbjct: 586 TSKELLEWGSRVSAANSRPPNP 606

BLAST of MS013916 vs. ExPASy Swiss-Prot
Match: Q9FFT4 (Pyruvate decarboxylase 2 OS=Arabidopsis thaliana OX=3702 GN=PDC2 PE=2 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 1.5e-234
Identity = 393/574 (68.47%), Postives = 463/574 (80.66%), Query Frame = 0

Query: 10  PIQIQTQSPPPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCSEL 69
           P+   T SP   TLG +LARRLVEIGV D+FSVPGD NL LLD+ +AE  L L+GCC+EL
Sbjct: 34  PLHSTTVSPCDATLGRYLARRLVEIGVTDVFSVPGDFNLTLLDHLIAEPNLKLIGCCNEL 93

Query: 70  NAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKI 129
           NAGYAADGYAR RGVGAC VTFTVGGLS++NA+AGAYSE+LP++CIVGGPNSNDYG+ +I
Sbjct: 94  NAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRI 153

Query: 130 IHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCNLV 189
           +HHTIGLPDF+QELRCFQ VTC+QA+I+N+EEA   ID AI   L+E KPVYISI CNL 
Sbjct: 154 LHHTIGLPDFTQELRCFQAVTCFQAVINNLEEAHELIDTAISTALKESKPVYISISCNLP 213

Query: 190 AIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQAA 249
           AIP P+FS    +P  L  K SNQ+GL+ A E AA+ LN AVKPV+V G K+R+AKA  A
Sbjct: 214 AIPLPTFS-RHPVPFMLPMKVSNQIGLDAAVEAAAEFLNKAVKPVLVGGPKMRVAKAADA 273

Query: 250 FVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLD 309
           FVELADA GY +AV PSAKG  PE+H HFIGTYWGAVSTAFC E VE ADA +FAG   +
Sbjct: 274 FVELADASGYGLAVMPSAKGQVPEHHKHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFN 333

Query: 310 ELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRR 369
           +  +V +SL  KK KAIIV+P+RV   NGP+FG +LMKDFL  LA+R+K N T+YENY R
Sbjct: 334 DYSSVGYSLLLKKEKAIIVQPDRVTIGNGPAFGCVLMKDFLSELAKRIKHNNTSYENYHR 393

Query: 370 IYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEV 429
           IYVPE  PL+  P E+LRVNVLF+HIQ MLSS   V++E GDSWF+CQKLKLP+ CGYE 
Sbjct: 394 IYVPEGKPLRDNPNESLRVNVLFQHIQNMLSSESAVLAETGDSWFNCQKLKLPEGCGYEF 453

Query: 430 QLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNG 489
           Q+ Y SIGWS+GATLGYAQA P +RV+ CIGDGSFQ++ QDVSTM+R GQK IIFLINNG
Sbjct: 454 QMQYGSIGWSVGATLGYAQAMPNRRVIACIGDGSFQVTAQDVSTMIRCGQKTIIFLINNG 513

Query: 490 GYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDC 549
           GYTIEVEIHDGPYNVIKNW+YTA V A+HN +GKCWTAKV  EEELVKAI TAT +  + 
Sbjct: 514 GYTIEVEIHDGPYNVIKNWNYTAFVEAIHNGEGKCWTAKVRCEEELVKAINTATNEEKES 573

Query: 550 LCFIEAILHRDDTSKELLEFGSKIAAISCRPPRP 584
            CFIE I+H+DDTSKELLE+GS+++A + RPP P
Sbjct: 574 FCFIEVIVHKDDTSKELLEWGSRVSAANSRPPNP 606

BLAST of MS013916 vs. ExPASy Swiss-Prot
Match: A2XFI3 (Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=2)

HSP 1 Score: 808.9 bits (2088), Expect = 3.7e-233
Identity = 380/562 (67.62%), Postives = 459/562 (81.67%), Query Frame = 0

Query: 22  TLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCSELNAGYAADGYARL 81
           +LG HLARRLV++GV D+F+VPGD NL LLD+ +AE GL LVGCC+ELNAGYAADGYAR 
Sbjct: 45  SLGRHLARRLVQVGVSDVFAVPGDFNLTLLDHLIAEPGLRLVGCCNELNAGYAADGYARA 104

Query: 82  RGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKIIHHTIGLPDFSQ 141
           RGVGACAVTFTVGGLS++NA+AGAYSE+LPV+CI GGPNSNDYG+ +I+HHTIGLPDFSQ
Sbjct: 105 RGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIAGGPNSNDYGTNRILHHTIGLPDFSQ 164

Query: 142 ELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCNLVAIPHPSFSMERL 201
           ELRCFQ VTC+QA++ N+E+A  +ID AI   L E KPVY+SI CNL  +PHP+FS +  
Sbjct: 165 ELRCFQTVTCHQAVVTNLEDAHEQIDTAIATALRESKPVYLSISCNLPGLPHPTFSRDP- 224

Query: 202 IPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQAAFVELADACGYAV 261
           +P  L P+ SN+MGLE A E   + LN AVKPV+V G KLR+AKA  AFV+L DA GYA 
Sbjct: 225 VPFFLAPRLSNKMGLEAAVEATVEFLNKAVKPVLVGGPKLRVAKAGKAFVDLVDASGYAY 284

Query: 262 AVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLDELETVAFSLTYK 321
           AV PSAKGL PE HPHFIGTYWGAVSTAFC E VE ADA +FAG   ++  +V +S   K
Sbjct: 285 AVMPSAKGLVPETHPHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSFLLK 344

Query: 322 KNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRRIYVPESGPLQSE 381
           K+KAIIV+PERV+  NGP+FG ++MK+FL  LA+R+  N TAYENY+RI+VPE  PL+SE
Sbjct: 345 KDKAIIVQPERVIVGNGPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLESE 404

Query: 382 PGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEVQLLYASIGWSLG 441
           P E LRVNVLFKH+QKML+S+  VI+E GDSWF+CQKLKLP+ CGYE Q+ Y SIGWS+G
Sbjct: 405 PNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVG 464

Query: 442 ATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNGGYTIEVEIHDGP 501
           A LGYAQ A  +RV+ CIGDGSFQ++ QDVSTM+R  Q +IIFLINNGGYTIEVEIHDGP
Sbjct: 465 ALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTIEVEIHDGP 524

Query: 502 YNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDCLCFIEAILHRDD 561
           YNVIKNW+YT +V+A+HN +GKCWT+KV  EEEL +AI  A  ++ DCLCFIE I H+DD
Sbjct: 525 YNVIKNWNYTGLVDAIHNGEGKCWTSKVKCEEELTEAIGMALGEKKDCLCFIEVIAHKDD 584

Query: 562 TSKELLEFGSKIAAISCRPPRP 584
           TSKELLE+GS+++A + RPP P
Sbjct: 585 TSKELLEWGSRVSAANSRPPNP 605

BLAST of MS013916 vs. ExPASy Swiss-Prot
Match: A2Y5L9 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 1.1e-232
Identity = 379/562 (67.44%), Postives = 460/562 (81.85%), Query Frame = 0

Query: 22  TLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCSELNAGYAADGYARL 81
           TLG HLARRLV+IG  D+F+VPGD NL LLDY +AE GL L+GCC+ELNAGYAADGYAR 
Sbjct: 44  TLGRHLARRLVQIGATDVFAVPGDFNLTLLDYLIAEPGLKLIGCCNELNAGYAADGYARA 103

Query: 82  RGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKIIHHTIGLPDFSQ 141
           RGVGACAVTFTVGGLS++NA+AGAYSE+LPV+CIVGGPNSNDYG+ +I+HHTIGLPDFSQ
Sbjct: 104 RGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQ 163

Query: 142 ELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCNLVAIPHPSFSMERL 201
           ELRCFQ +TCYQA+I+N+++A  +ID AI   L E KPVYIS+ CNL  + HP+FS E  
Sbjct: 164 ELRCFQTITCYQAVINNLDDAHEQIDTAIATALRESKPVYISVGCNLAGLSHPTFSREP- 223

Query: 202 IPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQAAFVELADACGYAV 261
           +PL + P+ SN+  LE A E AA  LN AVKPVMV G K+R+AKA+ AF  +A++ GY +
Sbjct: 224 VPLFISPRLSNKANLEYAVEAAADFLNKAVKPVMVGGPKIRVAKAKKAFAGIAESSGYPI 283

Query: 262 AVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLDELETVAFSLTYK 321
           AV PSAKGL PE+HP FIGTYWGAVST FC E VE ADA +FAG   ++  +V +SL  K
Sbjct: 284 AVMPSAKGLVPEHHPRFIGTYWGAVSTTFCAEIVESADAYLFAGPIFNDYSSVGYSLLLK 343

Query: 322 KNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRRIYVPESGPLQSE 381
           + KA+IV+P+RVV  NGP+FG ILM +FL ALA+RL  N TAY+NYRRI++P+  P   +
Sbjct: 344 REKAVIVQPDRVVVGNGPAFGCILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQ 403

Query: 382 PGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEVQLLYASIGWSLG 441
           P E LRVN+LFKHI++MLS +  VI+E GDSWF+CQKL+LP+ CGYE Q+ Y SIGWS+G
Sbjct: 404 PDEPLRVNILFKHIKEMLSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVG 463

Query: 442 ATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNGGYTIEVEIHDGP 501
           ATLGYAQAA  +RV+ CIGDGSFQM+ QDVSTMLR GQK+IIFLINNGGYTIEVEIHDGP
Sbjct: 464 ATLGYAQAAKDKRVISCIGDGSFQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGP 523

Query: 502 YNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDCLCFIEAILHRDD 561
           YNVIKNWDYT +++A+HN DG CWT KV TEEEL++AI TAT  + DCLCFIE I+H+DD
Sbjct: 524 YNVIKNWDYTGLIDAIHNSDGNCWTKKVRTEEELIEAIATATGAKKDCLCFIEIIVHKDD 583

Query: 562 TSKELLEFGSKIAAISCRPPRP 584
           TSKELLE+GS+++A + RPP P
Sbjct: 584 TSKELLEWGSRVSAANSRPPNP 604

BLAST of MS013916 vs. ExPASy TrEMBL
Match: A0A6J1CS04 (Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013786 PE=3 SV=1)

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 577/584 (98.80%), Postives = 578/584 (98.97%), Query Frame = 0

Query: 1   NGAAAPTNKPIQIQTQSPPPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGL 60
           NGAAAPTNKPIQIQTQSPPPWTLGH+LARRLVEIGVGDIFSVPGD NLLLLDYFVAE GL
Sbjct: 23  NGAAAPTNKPIQIQTQSPPPWTLGHYLARRLVEIGVGDIFSVPGDXNLLLLDYFVAEXGL 82

Query: 61  NLVGCCSELNAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPN 120
           NLVGCCSELNAGYAADGYAR RGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPN
Sbjct: 83  NLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPN 142

Query: 121 SNDYGSKKIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPV 180
           SNDYGSKKIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEE QWEIDRAICKCL ERKPV
Sbjct: 143 SNDYGSKKIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEETQWEIDRAICKCLGERKPV 202

Query: 181 YISICCNLVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKK 240
           YISICCNLVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKK
Sbjct: 203 YISICCNLVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKK 262

Query: 241 LRLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADA 300
           LR AKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADA
Sbjct: 263 LRPAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADA 322

Query: 301 SIFAGATLDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPN 360
           SIFAGATLDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPN
Sbjct: 323 SIFAGATLDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPN 382

Query: 361 PTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLK 420
           PTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLK
Sbjct: 383 PTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLK 442

Query: 421 LPKSCGYEVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQK 480
           LPKSCGYEVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQK
Sbjct: 443 LPKSCGYEVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQK 502

Query: 481 NIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIE 540
           NIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIE
Sbjct: 503 NIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIE 562

Query: 541 TATKDRNDCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRPL 585
           TATKDRNDCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRPL
Sbjct: 563 TATKDRNDCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRPL 606

BLAST of MS013916 vs. ExPASy TrEMBL
Match: A0A5D3E0N5 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold669G00060 PE=3 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.1e-288
Identity = 480/577 (83.19%), Postives = 524/577 (90.81%), Query Frame = 0

Query: 8   NKPIQIQTQSPPPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCS 67
           N  + I T +  P TLGH+LARRLV+IG+ DIFSVPGDSNL++ DYFVAEKGLNLVGCC+
Sbjct: 65  NGAVTIPTPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCN 124

Query: 68  ELNAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSK 127
           ELNAGYAADGYAR RGVGACAVTFTVG LSLINA+AGAYSEDLPV+CIVGGPNSNDYGSK
Sbjct: 125 ELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSK 184

Query: 128 KIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCN 187
           KI+HHTIGLPDF QELRCFQNVTCYQAIIDN+E+AQW+ID AI KCLEE KP+YISICCN
Sbjct: 185 KILHHTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCN 244

Query: 188 LVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQ 247
           LVAIPHPSFS + LIPLSL PKQSNQM LE A EKAAQLLN+A+KPVM+ GKKLR  K+Q
Sbjct: 245 LVAIPHPSFSAQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQ 304

Query: 248 AAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGAT 307
             F+ELA+ACGY VAVTPSAKG+FPENHPHFIG YWG VS+A+CGETVEIADASIF GAT
Sbjct: 305 EPFIELANACGYPVAVTPSAKGMFPENHPHFIGIYWGTVSSAYCGETVEIADASIFVGAT 364

Query: 308 LDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENY 367
           LDELETVAF+LTYKKNKAI+VE +RVVFPNGPSFGPILMKDFL ALA+RLKPN TAYENY
Sbjct: 365 LDELETVAFTLTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENY 424

Query: 368 RRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGY 427
           RRIY+ ESGPL+S PGEALRVN+LFKHIQKMLSSNM VISEAGDSWFHCQ+LKLPKSCGY
Sbjct: 425 RRIYIAESGPLESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGY 484

Query: 428 EVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLIN 487
           EVQLLYASIGWSLGATLGYAQAAP++RVVLCIGDGSFQM+PQDVSTM+ L Q NIIFLIN
Sbjct: 485 EVQLLYASIGWSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLIN 544

Query: 488 NGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRN 547
           NGGYTIEVEIHDGPYNVIKNWDYTA+V AMHN DG CWT KVHTEEEL+ AIE   ++R 
Sbjct: 545 NGGYTIEVEIHDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERK 604

Query: 548 DCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRPL 585
           D LCFIE I+HRDDTS+ELLEFGSK AA+S RPPRPL
Sbjct: 605 DYLCFIEVIVHRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of MS013916 vs. ExPASy TrEMBL
Match: A0A5A7T827 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold141G00370 PE=3 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 4.2e-288
Identity = 479/577 (83.02%), Postives = 524/577 (90.81%), Query Frame = 0

Query: 8   NKPIQIQTQSPPPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCS 67
           N  + I T +  P TLGH+LARRLV+IG+ DIFSVPGDSNL++ DYFVAEKGLNLVGCC+
Sbjct: 65  NGAVTIPTPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCN 124

Query: 68  ELNAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSK 127
           ELNAGYAADGYAR RGVGACAVTFTVG LSLINA+AGAYSEDLPV+CIVGGPNSNDYGSK
Sbjct: 125 ELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSK 184

Query: 128 KIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCN 187
           KI+HHTIGLPDF QELRCFQNVTCYQAIIDN+E+AQW+ID AI KCLEE KP+YISICCN
Sbjct: 185 KILHHTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCN 244

Query: 188 LVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQ 247
           LVAIPHPSFS + LIPLSL PKQSNQM LE A EKAAQLLN+A+KPVM+ GKKLR  K+Q
Sbjct: 245 LVAIPHPSFSAQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQ 304

Query: 248 AAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGAT 307
             F+ELA+ACGY VAVTPSAKG+FPE+HPHFIG YWG VS+A+CGETVEIADASIF GAT
Sbjct: 305 EPFIELANACGYPVAVTPSAKGMFPESHPHFIGIYWGTVSSAYCGETVEIADASIFVGAT 364

Query: 308 LDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENY 367
           LDELETVAF+LTYKKNKAI+VE +RVVFPNGPSFGPILMKDFL ALA+RLKPN TAYENY
Sbjct: 365 LDELETVAFTLTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENY 424

Query: 368 RRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGY 427
           RRIY+ ESGPL+S PGEALRVN+LFKHIQKMLSSNM VISEAGDSWFHCQ+LKLPKSCGY
Sbjct: 425 RRIYIAESGPLESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGY 484

Query: 428 EVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLIN 487
           EVQLLYASIGWSLGATLGYAQAAP++RVVLCIGDGSFQM+PQDVSTM+ L Q NIIFLIN
Sbjct: 485 EVQLLYASIGWSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLIN 544

Query: 488 NGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRN 547
           NGGYTIEVEIHDGPYNVIKNWDYTA+V AMHN DG CWT KVHTEEEL+ AIE   ++R 
Sbjct: 545 NGGYTIEVEIHDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERK 604

Query: 548 DCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRPL 585
           D LCFIE I+HRDDTS+ELLEFGSK AA+S RPPRPL
Sbjct: 605 DYLCFIEVIVHRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of MS013916 vs. ExPASy TrEMBL
Match: A0A1S3CKZ0 (Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502148 PE=3 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 4.2e-288
Identity = 479/577 (83.02%), Postives = 524/577 (90.81%), Query Frame = 0

Query: 8   NKPIQIQTQSPPPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCS 67
           N  + I T +  P TLGH+LARRLV+IG+ DIFSVPGDSNL++ DYFVAEKGLNLVGCC+
Sbjct: 65  NGAVTIPTPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCN 124

Query: 68  ELNAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSK 127
           ELNAGYAADGYAR RGVGACAVTFTVG LSLINA+AGAYSEDLPV+CIVGGPNSNDYGSK
Sbjct: 125 ELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSK 184

Query: 128 KIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCN 187
           KI+HHTIGLPDF QELRCFQNVTCYQAIIDN+E+AQW+ID AI KCLEE KP+YISICCN
Sbjct: 185 KILHHTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCN 244

Query: 188 LVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQ 247
           LVAIPHPSFS + LIPLSL PKQSNQM LE A EKAAQLLN+A+KPVM+ GKKLR  K+Q
Sbjct: 245 LVAIPHPSFSAQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQ 304

Query: 248 AAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGAT 307
             F+ELA+ACGY VAVTPSAKG+FPE+HPHFIG YWG VS+A+CGETVEIADASIF GAT
Sbjct: 305 EPFIELANACGYPVAVTPSAKGMFPESHPHFIGIYWGTVSSAYCGETVEIADASIFVGAT 364

Query: 308 LDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENY 367
           LDELETVAF+LTYKKNKAI+VE +RVVFPNGPSFGPILMKDFL ALA+RLKPN TAYENY
Sbjct: 365 LDELETVAFTLTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENY 424

Query: 368 RRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGY 427
           RRIY+ ESGPL+S PGEALRVN+LFKHIQKMLSSNM VISEAGDSWFHCQ+LKLPKSCGY
Sbjct: 425 RRIYIAESGPLESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGY 484

Query: 428 EVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLIN 487
           EVQLLYASIGWSLGATLGYAQAAP++RVVLCIGDGSFQM+PQDVSTM+ L Q NIIFLIN
Sbjct: 485 EVQLLYASIGWSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLIN 544

Query: 488 NGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRN 547
           NGGYTIEVEIHDGPYNVIKNWDYTA+V AMHN DG CWT KVHTEEEL+ AIE   ++R 
Sbjct: 545 NGGYTIEVEIHDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERK 604

Query: 548 DCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRPL 585
           D LCFIE I+HRDDTS+ELLEFGSK AA+S RPPRPL
Sbjct: 605 DYLCFIEVIVHRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of MS013916 vs. ExPASy TrEMBL
Match: A0A6J1GDK7 (Pyruvate decarboxylase OS=Cucurbita moschata OX=3662 GN=LOC111453217 PE=3 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 1.0e-286
Identity = 475/575 (82.61%), Postives = 528/575 (91.83%), Query Frame = 0

Query: 8   NKPIQIQTQSPPPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCS 67
           N  ++I      P TLGH+LARRLV+IGV DIFSVPGDSNL+LLDYFVAEKGLNLVGCCS
Sbjct: 31  NNGVEIVESMVTPSTLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAEKGLNLVGCCS 90

Query: 68  ELNAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSK 127
           ELNAGYAADGYAR RGVGAC VTFTVGGLSLINA+AGAYSEDLP++CIVG PNSN+YGS 
Sbjct: 91  ELNAGYAADGYARRRGVGACVVTFTVGGLSLINAIAGAYSEDLPLVCIVGAPNSNEYGSN 150

Query: 128 KIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCN 187
           KI+HHTIGLPDFSQELRCFQNVTCYQA+I+N+E+AQWEID AI KCLE+ KPVYISI CN
Sbjct: 151 KILHHTIGLPDFSQELRCFQNVTCYQAVINNLEDAQWEIDTAISKCLEQSKPVYISISCN 210

Query: 188 LVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQ 247
           L AIPHPSFSM++ +PLS+  KQSNQMGLE+A E AAQLLN+AVKPVMV GKKLR+AKAQ
Sbjct: 211 LAAIPHPSFSMKQFLPLSISSKQSNQMGLEMAVEMAAQLLNTAVKPVMVGGKKLRVAKAQ 270

Query: 248 AAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGAT 307
           AAFVELAD C YAVAVTPSAKG+FPENH HFIGTYWG++S+AFCGETVEIADASIFAGA 
Sbjct: 271 AAFVELADVCDYAVAVTPSAKGMFPENHRHFIGTYWGSLSSAFCGETVEIADASIFAGAI 330

Query: 308 LDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENY 367
            DELETV +SL+YKKNKAIIVE +RVVFPNGPSFGP+LMKDFLRALA+RLKPNPTAYENY
Sbjct: 331 FDELETVGYSLSYKKNKAIIVESDRVVFPNGPSFGPVLMKDFLRALAKRLKPNPTAYENY 390

Query: 368 RRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGY 427
            RIY+P+SGP QSEPGEALRVNVLFKHIQKMLS NM VISEAGDSWFHCQ L+LP+SCG+
Sbjct: 391 TRIYIPKSGPTQSEPGEALRVNVLFKHIQKMLSGNMAVISEAGDSWFHCQNLELPRSCGF 450

Query: 428 EVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLIN 487
           E+QL+YASIGWSLGATLGYAQA+P+ RVVLCIGDGSFQM+PQD+STMLRLGQ+NIIFLIN
Sbjct: 451 EMQLVYASIGWSLGATLGYAQASPEDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLIN 510

Query: 488 NGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRN 547
           NGGYTIEVEIHDGPYNVIKNW+YTA+V AMHNQ G CWT KV TEEELVKAIE AT D+N
Sbjct: 511 NGGYTIEVEIHDGPYNVIKNWNYTALVEAMHNQTGNCWTTKVETEEELVKAIEMATGDKN 570

Query: 548 DCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPR 583
           +CLCFIEAI+HRDDTSKELL+FG+K+AA + RPP+
Sbjct: 571 ECLCFIEAIVHRDDTSKELLDFGNKLAATNSRPPK 605

BLAST of MS013916 vs. TAIR 10
Match: AT5G01320.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 816.6 bits (2108), Expect = 1.3e-236
Identity = 392/582 (67.35%), Postives = 468/582 (80.41%), Query Frame = 0

Query: 6   PTNKPIQIQTQSP----PPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLN 65
           P+N    IQ  +P       TLG HL+RRLV+ GV D+FSVPGD NL LLD+ +AE  LN
Sbjct: 22  PSNAVATIQDSAPITTTSESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELN 81

Query: 66  LVGCCSELNAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNS 125
            +GCC+ELNAGYAADGYAR RGVGAC VTFTVGGLS++NA+AGAYSE+LPV+CIVGGPNS
Sbjct: 82  NIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNS 141

Query: 126 NDYGSKKIIHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVY 185
           ND+G+ +I+HHTIGLPDFSQELRCFQ VTCYQA+++N+E+A  +ID+AI   L+E KPVY
Sbjct: 142 NDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALKESKPVY 201

Query: 186 ISICCNLVAIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKL 245
           ISI CNL A PHP+F+ +  +P  L P+ SN MGLE A E   + LN AVKPVMV G KL
Sbjct: 202 ISISCNLAATPHPTFARDP-VPFDLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGPKL 261

Query: 246 RLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADAS 305
           R+AKA  AF+ELADA GY +AV PS KGL PENHPHFIGTYWGAVST FC E VE ADA 
Sbjct: 262 RVAKASEAFLELADASGYPLAVMPSTKGLVPENHPHFIGTYWGAVSTPFCSEIVESADAY 321

Query: 306 IFAGATLDELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNP 365
           IFAG   ++  +V +SL  KK KAIIV P+RVV  NGP+FG +LM DF R LA+R+K N 
Sbjct: 322 IFAGPIFNDYSSVGYSLLLKKEKAIIVHPDRVVVANGPTFGCVLMSDFFRELAKRVKRNE 381

Query: 366 TAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKL 425
           TAYENY RI+VPE  PL+ +PGE LRVN +F+HIQKMLSS   VI+E GDSWF+CQKLKL
Sbjct: 382 TAYENYERIFVPEGKPLKCKPGEPLRVNAMFQHIQKMLSSETAVIAETGDSWFNCQKLKL 441

Query: 426 PKSCGYEVQLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKN 485
           PK CGYE Q+ Y SIGWS+GATLGYAQA P++RV+  IGDGSFQ++ QD+STM+R GQK 
Sbjct: 442 PKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDISTMIRNGQKA 501

Query: 486 IIFLINNGGYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIET 545
           IIFLINNGGYTIEVEIHDGPYNVIKNW+YT +V+A+HN +GKCWT KV  EEELV+AI+T
Sbjct: 502 IIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAIKT 561

Query: 546 ATKDRNDCLCFIEAILHRDDTSKELLEFGSKIAAISCRPPRP 584
           AT ++ D LCFIE I+H+DDTSKELLE+GS+++A + RPP P
Sbjct: 562 ATTEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGRPPNP 602

BLAST of MS013916 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 816.2 bits (2107), Expect = 1.7e-236
Identity = 388/562 (69.04%), Postives = 464/562 (82.56%), Query Frame = 0

Query: 22  TLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCSELNAGYAADGYARL 81
           TLG HLARRLV+ GV D+FSVPGD NL LLD+ +AE  LNL+GCC+ELNAGYAADGYAR 
Sbjct: 46  TLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYARS 105

Query: 82  RGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKIIHHTIGLPDFSQ 141
           RGVGAC VTFTVGGLS++NA+AGAYSE+LP++CIVGGPNSNDYG+ +I+HHTIGLPDFSQ
Sbjct: 106 RGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFSQ 165

Query: 142 ELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCNLVAIPHPSFSMERL 201
           ELRCFQ VTCYQA+++N+++A  +ID+AI   L+E KPVYIS+ CNL AIPH +FS +  
Sbjct: 166 ELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKESKPVYISVSCNLAAIPHHTFSRDP- 225

Query: 202 IPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQAAFVELADACGYAV 261
           +P SL P+ SN+MGLE A E   + LN AVKPVMV G KLR+AKA  AFVELADA GYA+
Sbjct: 226 VPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELADASGYAL 285

Query: 262 AVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLDELETVAFSLTYK 321
           A+ PSAKG  PE+HPHFIGTYWGAVST FC E VE ADA IFAG   ++  +V +SL  K
Sbjct: 286 AMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLLK 345

Query: 322 KNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRRIYVPESGPLQSE 381
           K KAI+V+P+R+   NGP+FG ILM DF R L++R+K N TAYENY RI+VPE  PL+ E
Sbjct: 346 KEKAIVVQPDRITVANGPTFGCILMSDFFRELSKRVKRNETAYENYHRIFVPEGKPLKCE 405

Query: 382 PGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEVQLLYASIGWSLG 441
             E LRVN +F+HIQKMLSS   VI+E GDSWF+CQKLKLPK CGYE Q+ Y SIGWS+G
Sbjct: 406 SREPLRVNTMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVG 465

Query: 442 ATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNGGYTIEVEIHDGP 501
           ATLGYAQA+P++RV+  IGDGSFQ++ QD+STMLR GQK IIFLINNGGYTIEVEIHDGP
Sbjct: 466 ATLGYAQASPEKRVLAFIGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEVEIHDGP 525

Query: 502 YNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDCLCFIEAILHRDD 561
           YNVIKNW+YT +V+A+HN +G CWTAKV  EEELV+AI TAT ++ DCLCFIE ILH+DD
Sbjct: 526 YNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEELVEAITTATTEKKDCLCFIEVILHKDD 585

Query: 562 TSKELLEFGSKIAAISCRPPRP 584
           TSKELLE+GS+++A + RPP P
Sbjct: 586 TSKELLEWGSRVSAANSRPPNP 606

BLAST of MS013916 vs. TAIR 10
Match: AT5G54960.1 (pyruvate decarboxylase-2 )

HSP 1 Score: 813.5 bits (2100), Expect = 1.1e-235
Identity = 393/574 (68.47%), Postives = 463/574 (80.66%), Query Frame = 0

Query: 10  PIQIQTQSPPPWTLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCSEL 69
           P+   T SP   TLG +LARRLVEIGV D+FSVPGD NL LLD+ +AE  L L+GCC+EL
Sbjct: 34  PLHSTTVSPCDATLGRYLARRLVEIGVTDVFSVPGDFNLTLLDHLIAEPNLKLIGCCNEL 93

Query: 70  NAGYAADGYARLRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKI 129
           NAGYAADGYAR RGVGAC VTFTVGGLS++NA+AGAYSE+LP++CIVGGPNSNDYG+ +I
Sbjct: 94  NAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRI 153

Query: 130 IHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCNLV 189
           +HHTIGLPDF+QELRCFQ VTC+QA+I+N+EEA   ID AI   L+E KPVYISI CNL 
Sbjct: 154 LHHTIGLPDFTQELRCFQAVTCFQAVINNLEEAHELIDTAISTALKESKPVYISISCNLP 213

Query: 190 AIPHPSFSMERLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQAA 249
           AIP P+FS    +P  L  K SNQ+GL+ A E AA+ LN AVKPV+V G K+R+AKA  A
Sbjct: 214 AIPLPTFS-RHPVPFMLPMKVSNQIGLDAAVEAAAEFLNKAVKPVLVGGPKMRVAKAADA 273

Query: 250 FVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLD 309
           FVELADA GY +AV PSAKG  PE+H HFIGTYWGAVSTAFC E VE ADA +FAG   +
Sbjct: 274 FVELADASGYGLAVMPSAKGQVPEHHKHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFN 333

Query: 310 ELETVAFSLTYKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRR 369
           +  +V +SL  KK KAIIV+P+RV   NGP+FG +LMKDFL  LA+R+K N T+YENY R
Sbjct: 334 DYSSVGYSLLLKKEKAIIVQPDRVTIGNGPAFGCVLMKDFLSELAKRIKHNNTSYENYHR 393

Query: 370 IYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEV 429
           IYVPE  PL+  P E+LRVNVLF+HIQ MLSS   V++E GDSWF+CQKLKLP+ CGYE 
Sbjct: 394 IYVPEGKPLRDNPNESLRVNVLFQHIQNMLSSESAVLAETGDSWFNCQKLKLPEGCGYEF 453

Query: 430 QLLYASIGWSLGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNG 489
           Q+ Y SIGWS+GATLGYAQA P +RV+ CIGDGSFQ++ QDVSTM+R GQK IIFLINNG
Sbjct: 454 QMQYGSIGWSVGATLGYAQAMPNRRVIACIGDGSFQVTAQDVSTMIRCGQKTIIFLINNG 513

Query: 490 GYTIEVEIHDGPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDC 549
           GYTIEVEIHDGPYNVIKNW+YTA V A+HN +GKCWTAKV  EEELVKAI TAT +  + 
Sbjct: 514 GYTIEVEIHDGPYNVIKNWNYTAFVEAIHNGEGKCWTAKVRCEEELVKAINTATNEEKES 573

Query: 550 LCFIEAILHRDDTSKELLEFGSKIAAISCRPPRP 584
            CFIE I+H+DDTSKELLE+GS+++A + RPP P
Sbjct: 574 FCFIEVIVHKDDTSKELLEWGSRVSAANSRPPNP 606

BLAST of MS013916 vs. TAIR 10
Match: AT5G01330.1 (pyruvate decarboxylase-3 )

HSP 1 Score: 797.7 bits (2059), Expect = 6.1e-231
Identity = 382/562 (67.97%), Postives = 455/562 (80.96%), Query Frame = 0

Query: 22  TLGHHLARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCSELNAGYAADGYARL 81
           TLG HL+RRLV+ GV DIF+VPGD NL LLD  +A   LN +GCC+ELNAGYAADGYAR 
Sbjct: 31  TLGRHLSRRLVQAGVTDIFTVPGDFNLSLLDQLIANPELNNIGCCNELNAGYAADGYARS 90

Query: 82  RGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKIIHHTIGLPDFSQ 141
           RGVGAC VTFTVGGLS++NA+AGAYSE+LPV+CIVGGPNSND+G+ +I+HHTIGLPDFSQ
Sbjct: 91  RGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLPDFSQ 150

Query: 142 ELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEERKPVYISICCNLVAIPHPSFSMERL 201
           ELRCFQ VTCYQA+++++E+A  +ID+AI   L E KPVYISI CNL AIPHP+F+    
Sbjct: 151 ELRCFQTVTCYQAVVNHLEDAHEQIDKAIATALRESKPVYISISCNLAAIPHPTFA-SYP 210

Query: 202 IPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRLAKAQAAFVELADACGYAV 261
           +P  L P+ SN+  LE A E   + LN AVKPVMV G KLR+AKA+ AFVELADA GY V
Sbjct: 211 VPFDLTPRLSNKDCLEAAVEATLEFLNKAVKPVMVGGPKLRVAKARDAFVELADASGYPV 270

Query: 262 AVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLDELETVAFSLTYK 321
           AV PSAKG  PENHPHFIGTYWGAVST FC E VE ADA IFAG   ++  +V +SL  K
Sbjct: 271 AVMPSAKGFVPENHPHFIGTYWGAVSTLFCSEIVESADAYIFAGPIFNDYSSVGYSLLLK 330

Query: 322 KNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRRIYVPESGPLQSE 381
           K KAIIV P+ VV  NGP+FG + M +F R LA+R+KPN TAYENY RI+VPE  PL+ +
Sbjct: 331 KEKAIIVHPDSVVVANGPTFGCVRMSEFFRELAKRVKPNKTAYENYHRIFVPEGKPLKCK 390

Query: 382 PGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEVQLLYASIGWSLG 441
           P E LR+N +F+HIQKMLS+   VI+E GDSWF+CQKLKLPK CGYE Q+ Y SIGWS+G
Sbjct: 391 PREPLRINAMFQHIQKMLSNETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVG 450

Query: 442 ATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNGGYTIEVEIHDGP 501
           ATLGYAQA P++RV+  IGDGSFQ++ QDVSTM+R GQK IIFLINNGGYTIEVEIHDGP
Sbjct: 451 ATLGYAQATPEKRVLSFIGDGSFQVTAQDVSTMIRNGQKTIIFLINNGGYTIEVEIHDGP 510

Query: 502 YNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDCLCFIEAILHRDD 561
           YNVIKNW+YT +V+A+HN +GKCWT KV  EEELV+AI TAT ++ D LCFIE I+H+DD
Sbjct: 511 YNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAINTATLEKKDSLCFIEVIVHKDD 570

Query: 562 TSKELLEFGSKIAAISCRPPRP 584
           TSKELLE+GS+++A + RPP P
Sbjct: 571 TSKELLEWGSRVSAANGRPPNP 591

BLAST of MS013916 vs. TAIR 10
Match: AT5G17380.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 85.5 bits (210), Expect = 1.5e-16
Identity = 116/507 (22.88%), Postives = 209/507 (41.22%), Query Frame = 0

Query: 14  QTQSPPPWTLGHHL-ARRLVEIGVGDIFSVPGDSNLLLLDYFVAEKGLNLVGCCSELNAG 73
           ++++ PP   G+ L A+ L  +GV  +F V G     L    +A  G+  +   +E +AG
Sbjct: 4   KSETTPPSIDGNVLVAKSLSHLGVTHMFGVVGIPVTSLASRAMA-LGIRFIAFHNEQSAG 63

Query: 74  YAADGYARLRGVGACAVTFTVGGLSLINAVAG---AYSEDLPVLCIVGGPNSNDYGSKKI 133
           YAA  Y  L   G   +  TV G   ++ +AG   A+    P++ I G  +  D      
Sbjct: 64  YAASAYGYL--TGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCDQRD------ 123

Query: 134 IHHTIGLPDFSQELRCFQNVTCYQAIIDNVEEAQWEIDRAICKCLEER---KP--VYISI 193
               +G  DF QEL   + V  +  + +  ++ + EI   + + L+     +P   Y+ I
Sbjct: 124 ----VGRGDF-QELDQIEAVKAFSKLSEKAKDVR-EIPDCVSRVLDRAVSGRPGGCYLDI 183

Query: 194 CCNLVAIPHPSFSMERLIPLSLHPKQSNQM--GLEVAAEKAAQLLNSAVKPVMVAGKKLR 253
             +++         ++L+      ++   +   L    E A  LL  A +P++V GK   
Sbjct: 184 PTDVLRQKISESEADKLVDEVERSRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAA 243

Query: 254 LAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASI 313
            ++A+    +L +  G     TP  KGL P+ H  F  T   +++   C       D ++
Sbjct: 244 YSRAEDELKKLVEITGIPFLPTPMGKGLLPDTH-EFSATAARSLAIGKC-------DVAL 303

Query: 314 FAGATLDELETVAFSLTYKKN-KAIIVE-PERVVFPNGPSFGPI-LMKDFLRALAQRLKP 373
             GA L+ L     S  + K+ K I+V+  E  +    P  G +   K  +  L + +K 
Sbjct: 304 VVGARLNWLLHFGESPKWDKDVKFILVDVSEEEIELRKPHLGIVGDAKTVIGLLNREIKD 363

Query: 374 NPTAYENYRRIYVPESGPLQSEPGEALRV---------------NVLFKHIQKMLSSNMT 433
           +P         +V        E GE + +                ++   I  +   +  
Sbjct: 364 DPFCL-GKSNSWVESISKKAKENGEKMEIQLAKDVVPFNFLTPMRIIRDAILAVEGPSPV 423

Query: 434 VISEAGDSWFHCQKLKLPKSCGYEVQL-LYASIGWSLGATLGYAQAAPQQRVVLCIGDGS 491
           V+SE  ++    + + + K     +    + ++G  LG  +  A A+P + VV   GD  
Sbjct: 424 VVSEGANTMDVGRSVLVQKEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSG 483