MS013800 (gene) Bitter gourd (TR) v1

Overview
NameMS013800
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptiontRNA-uridine aminocarboxypropyltransferase
Locationscaffold541: 45879 .. 46664 (+)
RNA-Seq ExpressionMS013800
SyntenyMS013800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCCGATGAACCATCACGACAACAACTCCGGCGGAGGCGGAGGCGGAGGCGGCGGCGGTAATTCCTCCTCCTCCTCAGCGCCGCCGCGGCGGTCAATTTGCCCTAATTGCTCCAGGCCGGAACCAGTATGCCTCTGCAAATTCTTACCGTCTCCACCAATCGCAACCAGAACCAAAATCATTATCCTCCAGCATCCTCACGAGGCTCGCCACAAGCTCTCCACCACACCAGTCCTTGGGAACTGCCTCCTCAACGCCACCACCATCGTCGGCCGCCGCCTCCGGCCAGGCCTCTCCGAACTCCTCGACCAGTCGCCTCCCGCCGTCTACCTCTTCCCGCCGTCCGCCGCCTCTCCAGCCGTCGACATATCGAGCCTCGACTCCTCGTCTCCGATCCGGCGCGGAGATTCCGATCTGGTCCTCATCGCGTTCGACGGAACGTGGAAGCACGCGAAGGAGATGGTGCGGTCGAGCGAGGAGTTCCTGTCGAAGTTCGCGACGAGAGTTTGCCTGGATTCCGACGAGAATGTGGACGGCGGAAGCATCTACGATTCGGAGCTGATTCTGAGGAAGCAGCCGCACGGCGGCTGCGTGAGCACGATGGAGGCGGTGGCGCGGTGTTTGGGAGCGGTGGAATCGAACGGGGCGGAGATTGAGAGGAGATTGATCGGAGTTTTGAGGGAAATGGTTAGGTTTCAGGCCGGCTACTTGAAGCCCATGAACCCTAGGCCCAAATTGAAGAACAGCAAGAACAAGAAGAAGAACAAGGAGAATGAAATTGCA

mRNA sequence

ATGGAGCCGATGAACCATCACGACAACAACTCCGGCGGAGGCGGAGGCGGAGGCGGCGGCGGTAATTCCTCCTCCTCCTCAGCGCCGCCGCGGCGGTCAATTTGCCCTAATTGCTCCAGGCCGGAACCAGTATGCCTCTGCAAATTCTTACCGTCTCCACCAATCGCAACCAGAACCAAAATCATTATCCTCCAGCATCCTCACGAGGCTCGCCACAAGCTCTCCACCACACCAGTCCTTGGGAACTGCCTCCTCAACGCCACCACCATCGTCGGCCGCCGCCTCCGGCCAGGCCTCTCCGAACTCCTCGACCAGTCGCCTCCCGCCGTCTACCTCTTCCCGCCGTCCGCCGCCTCTCCAGCCGTCGACATATCGAGCCTCGACTCCTCGTCTCCGATCCGGCGCGGAGATTCCGATCTGGTCCTCATCGCGTTCGACGGAACGTGGAAGCACGCGAAGGAGATGGTGCGGTCGAGCGAGGAGTTCCTGTCGAAGTTCGCGACGAGAGTTTGCCTGGATTCCGACGAGAATGTGGACGGCGGAAGCATCTACGATTCGGAGCTGATTCTGAGGAAGCAGCCGCACGGCGGCTGCGTGAGCACGATGGAGGCGGTGGCGCGGTGTTTGGGAGCGGTGGAATCGAACGGGGCGGAGATTGAGAGGAGATTGATCGGAGTTTTGAGGGAAATGGTTAGGTTTCAGGCCGGCTACTTGAAGCCCATGAACCCTAGGCCCAAATTGAAGAACAGCAAGAACAAGAAGAAGAACAAGGAGAATGAAATTGCA

Coding sequence (CDS)

ATGGAGCCGATGAACCATCACGACAACAACTCCGGCGGAGGCGGAGGCGGAGGCGGCGGCGGTAATTCCTCCTCCTCCTCAGCGCCGCCGCGGCGGTCAATTTGCCCTAATTGCTCCAGGCCGGAACCAGTATGCCTCTGCAAATTCTTACCGTCTCCACCAATCGCAACCAGAACCAAAATCATTATCCTCCAGCATCCTCACGAGGCTCGCCACAAGCTCTCCACCACACCAGTCCTTGGGAACTGCCTCCTCAACGCCACCACCATCGTCGGCCGCCGCCTCCGGCCAGGCCTCTCCGAACTCCTCGACCAGTCGCCTCCCGCCGTCTACCTCTTCCCGCCGTCCGCCGCCTCTCCAGCCGTCGACATATCGAGCCTCGACTCCTCGTCTCCGATCCGGCGCGGAGATTCCGATCTGGTCCTCATCGCGTTCGACGGAACGTGGAAGCACGCGAAGGAGATGGTGCGGTCGAGCGAGGAGTTCCTGTCGAAGTTCGCGACGAGAGTTTGCCTGGATTCCGACGAGAATGTGGACGGCGGAAGCATCTACGATTCGGAGCTGATTCTGAGGAAGCAGCCGCACGGCGGCTGCGTGAGCACGATGGAGGCGGTGGCGCGGTGTTTGGGAGCGGTGGAATCGAACGGGGCGGAGATTGAGAGGAGATTGATCGGAGTTTTGAGGGAAATGGTTAGGTTTCAGGCCGGCTACTTGAAGCCCATGAACCCTAGGCCCAAATTGAAGAACAGCAAGAACAAGAAGAAGAACAAGGAGAATGAAATTGCA

Protein sequence

MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTKIIILQHPHEARHKLSTTPVLGNCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSAASPAVDISSLDSSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDENVDGGSIYDSELILRKQPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGYLKPMNPRPKLKNSKNKKKNKENEIA
Homology
BLAST of MS013800 vs. NCBI nr
Match: XP_022152239.1 (DTW domain-containing protein 2 [Momordica charantia])

HSP 1 Score: 510.8 bits (1314), Expect = 7.2e-141
Identity = 258/262 (98.47%), Postives = 261/262 (99.62%), Query Frame = 0

Query: 1   MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK 60
           MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK
Sbjct: 1   MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK 60

Query: 61  IIILQHPHEARHKLSTTPVLGNCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSAASP 120
           IIILQHPHEARHKLSTTPVLG CLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSAASP
Sbjct: 61  IIILQHPHEARHKLSTTPVLGKCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSAASP 120

Query: 121 AVDISSLDSSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDENVDG 180
           AVDISSLDSSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSD+NVDG
Sbjct: 121 AVDISSLDSSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDDNVDG 180

Query: 181 GSIYDSELILRKQPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGYLKP 240
           GSIYDSELILRK+PHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGY+KP
Sbjct: 181 GSIYDSELILRKEPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGYVKP 240

Query: 241 MNPRPKLKNSKNKKKNKENEIA 263
           MNPRPKLKNSKNKKKNKENEIA
Sbjct: 241 MNPRPKLKNSKNKKKNKENEIA 262

BLAST of MS013800 vs. NCBI nr
Match: XP_023544253.1 (DTW domain-containing protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 372.5 bits (955), Expect = 3.0e-99
Identity = 197/268 (73.51%), Postives = 227/268 (84.70%), Query Frame = 0

Query: 1   MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK 60
           MEP NHH N++        G +S++SS P RRSICPNC+RP+PVCLCKFLPSPPIAT+T+
Sbjct: 1   MEPTNHHHNSA-------AGYSSAASSPPARRSICPNCTRPQPVCLCKFLPSPPIATKTQ 60

Query: 61  IIILQHPHEARHKLSTTPVLGNCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSA-AS 120
           IIILQHPHEA+HKLSTTP+L  CLLNATTIV RRL+PGLS+LLD+SP AVYLFPP+  +S
Sbjct: 61  IIILQHPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSS 120

Query: 121 PAVDISSLD---SSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDE 180
           P ++IS LD   SSSP    DS+LVLIAFDGTWKHAKEMVRSSEEFLS+FA R+CLD DE
Sbjct: 121 PPINISELDLTSSSSP----DSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDE 180

Query: 181 NVDGGSIYDSELILRKQPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAG 240
           +VDGGSI+DSELILRK+PHGGCVSTMEAVARCL  +E++GAEIE RLIGVLREMVR QAG
Sbjct: 181 SVDGGSIFDSELILRKEPHGGCVSTMEAVARCLRVMEADGAEIEVRLIGVLREMVRLQAG 240

Query: 241 YLKPMNPRPKLKNSKNKKKNK---ENEI 262
           +LKPMNPRPKL  +K K KNK   E+EI
Sbjct: 241 FLKPMNPRPKLLKTKKKMKNKSEREDEI 257

BLAST of MS013800 vs. NCBI nr
Match: XP_022928716.1 (DTW domain-containing protein 2 [Cucurbita moschata])

HSP 1 Score: 371.3 bits (952), Expect = 6.8e-99
Identity = 196/268 (73.13%), Postives = 223/268 (83.21%), Query Frame = 0

Query: 1   MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK 60
           MEP NHH              NS++SS P RRSICPNC+RP+PVCLCKFLPSPPIAT+T+
Sbjct: 1   MEPTNHHH-------------NSAASSPPARRSICPNCTRPQPVCLCKFLPSPPIATKTQ 60

Query: 61  IIILQHPHEARHKLSTTPVLGNCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSA-AS 120
           IIILQHPHEA+HKLSTTP+L  CLLNATTIV RRL+PGLS+LLD+SP AVYLFPP+  +S
Sbjct: 61  IIILQHPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSS 120

Query: 121 PAVDISSLD---SSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDE 180
           P ++IS LD   SSSP    DS+LVLIAFDGTWKHAKEMVRSSEEFLS+FA R+CLD DE
Sbjct: 121 PPINISELDLTSSSSP----DSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDE 180

Query: 181 NVDGGSIYDSELILRKQPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAG 240
           +VDGGSI+DSELILRK+PHGGCVSTMEAVARCL  +E++GAEIE RLIGVLREMVR QAG
Sbjct: 181 SVDGGSIFDSELILRKEPHGGCVSTMEAVARCLRVMEADGAEIEVRLIGVLREMVRLQAG 240

Query: 241 YLKPMNPRPKLKNSKNKKKNK---ENEI 262
           +LKPMNPRPKL  +K K KNK   E+EI
Sbjct: 241 FLKPMNPRPKLLKTKKKTKNKSEREDEI 251

BLAST of MS013800 vs. NCBI nr
Match: XP_022967888.1 (DTW domain-containing protein 2 [Cucurbita maxima])

HSP 1 Score: 369.8 bits (948), Expect = 2.0e-98
Identity = 192/262 (73.28%), Postives = 224/262 (85.50%), Query Frame = 0

Query: 1   MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK 60
           MEP NHH N++        G +S+SSS PPRRSICPNC+RP+ VCLCKFLPSPPIAT+T+
Sbjct: 1   MEPTNHHHNSA-------AGYSSASSSPPPRRSICPNCTRPQLVCLCKFLPSPPIATKTQ 60

Query: 61  IIILQHPHEARHKLSTTPVLGNCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSA-AS 120
           IIILQHPHEA+HKLSTTP+L  CLLNATTIV RRL+PGLS+LLD+SP AVYLFPP+  +S
Sbjct: 61  IIILQHPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSS 120

Query: 121 PAVDISSLDSSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDENVD 180
           P ++IS LD +S     DS+LVLIAFDGTWKHAKEMVRSSEEFLS+FA R+CLD DE+VD
Sbjct: 121 PPINISELDLTSS-SSSDSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDESVD 180

Query: 181 GGSIYDSELILRKQPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGYLK 240
           GG+I+DSELILRK+PHGGCVSTMEAVARCL  +E++GAEIE RLIGVLREMVR QAG+LK
Sbjct: 181 GGTIFDSELILRKEPHGGCVSTMEAVARCLRVMEADGAEIELRLIGVLREMVRLQAGFLK 240

Query: 241 PMNPRPKLKNSKNKKKNKENEI 262
           PMNPRPKL   KNK + +E+EI
Sbjct: 241 PMNPRPKLVKMKNKSE-REDEI 253

BLAST of MS013800 vs. NCBI nr
Match: KAG7033522.1 (DTW domain-containing protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 366.7 bits (940), Expect = 1.7e-97
Identity = 194/268 (72.39%), Postives = 221/268 (82.46%), Query Frame = 0

Query: 1   MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK 60
           ME  NHH              NS++S  P RRSICPNC+RP+PVCLCKFLPSPPIAT+T+
Sbjct: 1   MESTNHHH-------------NSAASPPPARRSICPNCTRPQPVCLCKFLPSPPIATKTQ 60

Query: 61  IIILQHPHEARHKLSTTPVLGNCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSA-AS 120
           IIILQHPHEA+HKLSTTP+L  CLLNATTIV RRL+PGLS+LLD+SP AVYLFPP+  +S
Sbjct: 61  IIILQHPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSS 120

Query: 121 PAVDISSLD---SSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDE 180
           P ++IS LD   SSSP    DS+LVLIAFDGTWKHAKEMVRSSEEFLS+FA R+CLD DE
Sbjct: 121 PPINISELDLTSSSSP----DSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDE 180

Query: 181 NVDGGSIYDSELILRKQPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAG 240
           +VDGGSI+DSELILRK+PHGGCVSTMEAVARCL  +E++GAEIE RLIGVLREMVR QAG
Sbjct: 181 SVDGGSIFDSELILRKEPHGGCVSTMEAVARCLRVMEADGAEIELRLIGVLREMVRLQAG 240

Query: 241 YLKPMNPRPKLKNSKNKKKNK---ENEI 262
           +LKPMNPRPKL  +K K KNK   E+EI
Sbjct: 241 FLKPMNPRPKLLKTKKKTKNKSEREDEI 251

BLAST of MS013800 vs. ExPASy Swiss-Prot
Match: Q8NBA8 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Homo sapiens OX=9606 GN=DTWD2 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.7e-15
Identity = 74/246 (30.08%), Postives = 111/246 (45.12%), Query Frame = 0

Query: 31  RRSICPNCSRPEPVCLCKFLPSPPIATRTKIIILQHPHEARHKLSTTPVLGNCLL--NAT 90
           RR  C  CSRP+ VCLC FLP+ P+   T + I+QHP E    L T P+L  CL      
Sbjct: 65  RRPECTRCSRPQKVCLCPFLPAHPLHISTHLYIIQHPAEENKVLRTVPLLAACLPQDKCK 124

Query: 91  TIVGRRLR----PGLSELLDQSPPAVYLFPPSAASPAVDISSLDSSSPIRRGDSDLVLIA 150
             +GRR      P LS +  +S   + L+P    + A ++      SP+        +I 
Sbjct: 125 VKIGRRFSEERDPELSTVCRKSGTLI-LYP---GAEAANLEEFILDSPVYPS----TIII 184

Query: 151 FDGTWKHAKEMVRSSEEFLSKFATRVCLDSDENVDGGSIYDSELILRKQPHGGCVSTMEA 210
            DGTW  AK++   +  F             + V   +   S+ ++R QP   C+ST+E 
Sbjct: 185 IDGTWSQAKDIFYKNSLF----------RHPKQVQLKTSISSQYVIRMQPTNRCLSTLEC 244

Query: 211 VARCLGAVESNGAEIERRLIGVLREMVRFQA--GYLKPMNPRPKLKNS-------KNKKK 262
            A  L  +E N   I+  L+  L+ +  FQ   G    ++    LKN        KNK+K
Sbjct: 245 AAVALSILEKNN-YIQETLLRPLQALCSFQLQHGAQIRLSKEHLLKNGLYPKPMPKNKRK 291

BLAST of MS013800 vs. ExPASy Swiss-Prot
Match: Q4R7M4 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Macaca fascicularis OX=9541 GN=DTWD2 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.7e-15
Identity = 74/246 (30.08%), Postives = 111/246 (45.12%), Query Frame = 0

Query: 31  RRSICPNCSRPEPVCLCKFLPSPPIATRTKIIILQHPHEARHKLSTTPVLGNCLL--NAT 90
           RR  C  CSRP+ VCLC FLP+ P+   T + I+QHP E    L T P+L  CL      
Sbjct: 65  RRPECSRCSRPQKVCLCPFLPAHPLHISTHLYIIQHPAEENKVLRTVPLLAACLPQDKCK 124

Query: 91  TIVGRRLR----PGLSELLDQSPPAVYLFPPSAASPAVDISSLDSSSPIRRGDSDLVLIA 150
             +GRR      P LS +  +S   + L+P    + A ++      SP+        +I 
Sbjct: 125 VKIGRRFSEERDPELSTVCRKSGTLI-LYP---GAEAANLEEFILDSPVYPS----TIII 184

Query: 151 FDGTWKHAKEMVRSSEEFLSKFATRVCLDSDENVDGGSIYDSELILRKQPHGGCVSTMEA 210
            DGTW  AK++   +  F             + V   +   S+ ++R QP   C+ST+E 
Sbjct: 185 IDGTWSQAKDIFYKNSLF----------RHPKQVQLKTSISSQYVIRMQPTNRCLSTLEC 244

Query: 211 VARCLGAVESNGAEIERRLIGVLREMVRFQA--GYLKPMNPRPKLKNS-------KNKKK 262
            A  L  +E N   I+  L+  L+ +  FQ   G    ++    LKN        KNK+K
Sbjct: 245 AAVALSILEKNN-YIQETLLRPLQALCSFQLQHGAQIRLSKEHLLKNGLYTKPMPKNKRK 291

BLAST of MS013800 vs. ExPASy Swiss-Prot
Match: Q9D0U1 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Mus musculus OX=10090 GN=Dtwd2 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.2e-13
Identity = 75/267 (28.09%), Postives = 114/267 (42.70%), Query Frame = 0

Query: 18  GGGGNSSSSSA--------PPRRSICPNCSRPEPVCLCKFLPSPPIATRTKIIILQHPHE 77
           G  G+S+S+            RR  C  CSRP+ VCLC +LP  P+   T + I+QHP E
Sbjct: 44  GASGDSTSADGLWGLPVEHAERRPECGRCSRPQKVCLCPYLPVRPLQISTHLYIIQHPAE 103

Query: 78  ARHKLSTTPVLGNCLL--NATTIVGRRL---RPGLSELLDQSPPAVYLFPPSAASPAVDI 137
               L T P+L  CL     T  +GRR    R      + +    + L+P    + A ++
Sbjct: 104 ESRVLRTVPLLAACLPPDRCTVKIGRRFSEERDVELATVCRDSGTLILYP---GAEATNL 163

Query: 138 SSLDSSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEF----LSKFATRVCLDSDENVDG 197
                 SP+    S ++LI  DGTW  AK++   +  F      +  T VC         
Sbjct: 164 EEFILDSPVY--PSTIILI--DGTWSQAKDIFYKNSLFRLPKQVQLKTSVC--------- 223

Query: 198 GSIYDSELILRKQPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGY--- 256
                S+ ++R QP   C+ST+E  A  L  +E N   I+  L+  L+ +  FQ  +   
Sbjct: 224 -----SQYVIRMQPTNRCLSTLECAAVALSILEKNNC-IQETLLRPLQALCSFQLQHGAQ 283

BLAST of MS013800 vs. ExPASy TrEMBL
Match: A0A6J1DFG5 (tRNA-uridine aminocarboxypropyltransferase OS=Momordica charantia OX=3673 GN=LOC111020010 PE=4 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 3.5e-141
Identity = 258/262 (98.47%), Postives = 261/262 (99.62%), Query Frame = 0

Query: 1   MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK 60
           MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK
Sbjct: 1   MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK 60

Query: 61  IIILQHPHEARHKLSTTPVLGNCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSAASP 120
           IIILQHPHEARHKLSTTPVLG CLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSAASP
Sbjct: 61  IIILQHPHEARHKLSTTPVLGKCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSAASP 120

Query: 121 AVDISSLDSSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDENVDG 180
           AVDISSLDSSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSD+NVDG
Sbjct: 121 AVDISSLDSSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDDNVDG 180

Query: 181 GSIYDSELILRKQPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGYLKP 240
           GSIYDSELILRK+PHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGY+KP
Sbjct: 181 GSIYDSELILRKEPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGYVKP 240

Query: 241 MNPRPKLKNSKNKKKNKENEIA 263
           MNPRPKLKNSKNKKKNKENEIA
Sbjct: 241 MNPRPKLKNSKNKKKNKENEIA 262

BLAST of MS013800 vs. ExPASy TrEMBL
Match: A0A6J1EL33 (tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111435542 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 3.3e-99
Identity = 196/268 (73.13%), Postives = 223/268 (83.21%), Query Frame = 0

Query: 1   MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK 60
           MEP NHH              NS++SS P RRSICPNC+RP+PVCLCKFLPSPPIAT+T+
Sbjct: 1   MEPTNHHH-------------NSAASSPPARRSICPNCTRPQPVCLCKFLPSPPIATKTQ 60

Query: 61  IIILQHPHEARHKLSTTPVLGNCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSA-AS 120
           IIILQHPHEA+HKLSTTP+L  CLLNATTIV RRL+PGLS+LLD+SP AVYLFPP+  +S
Sbjct: 61  IIILQHPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSS 120

Query: 121 PAVDISSLD---SSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDE 180
           P ++IS LD   SSSP    DS+LVLIAFDGTWKHAKEMVRSSEEFLS+FA R+CLD DE
Sbjct: 121 PPINISELDLTSSSSP----DSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDE 180

Query: 181 NVDGGSIYDSELILRKQPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAG 240
           +VDGGSI+DSELILRK+PHGGCVSTMEAVARCL  +E++GAEIE RLIGVLREMVR QAG
Sbjct: 181 SVDGGSIFDSELILRKEPHGGCVSTMEAVARCLRVMEADGAEIEVRLIGVLREMVRLQAG 240

Query: 241 YLKPMNPRPKLKNSKNKKKNK---ENEI 262
           +LKPMNPRPKL  +K K KNK   E+EI
Sbjct: 241 FLKPMNPRPKLLKTKKKTKNKSEREDEI 251

BLAST of MS013800 vs. ExPASy TrEMBL
Match: A0A6J1HWG2 (tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111467264 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 9.6e-99
Identity = 192/262 (73.28%), Postives = 224/262 (85.50%), Query Frame = 0

Query: 1   MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK 60
           MEP NHH N++        G +S+SSS PPRRSICPNC+RP+ VCLCKFLPSPPIAT+T+
Sbjct: 1   MEPTNHHHNSA-------AGYSSASSSPPPRRSICPNCTRPQLVCLCKFLPSPPIATKTQ 60

Query: 61  IIILQHPHEARHKLSTTPVLGNCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSA-AS 120
           IIILQHPHEA+HKLSTTP+L  CLLNATTIV RRL+PGLS+LLD+SP AVYLFPP+  +S
Sbjct: 61  IIILQHPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSS 120

Query: 121 PAVDISSLDSSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDENVD 180
           P ++IS LD +S     DS+LVLIAFDGTWKHAKEMVRSSEEFLS+FA R+CLD DE+VD
Sbjct: 121 PPINISELDLTSS-SSSDSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDESVD 180

Query: 181 GGSIYDSELILRKQPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGYLK 240
           GG+I+DSELILRK+PHGGCVSTMEAVARCL  +E++GAEIE RLIGVLREMVR QAG+LK
Sbjct: 181 GGTIFDSELILRKEPHGGCVSTMEAVARCLRVMEADGAEIELRLIGVLREMVRLQAGFLK 240

Query: 241 PMNPRPKLKNSKNKKKNKENEI 262
           PMNPRPKL   KNK + +E+EI
Sbjct: 241 PMNPRPKLVKMKNKSE-REDEI 253

BLAST of MS013800 vs. ExPASy TrEMBL
Match: A0A5D3D883 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold307G00010 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 3.0e-92
Identity = 184/262 (70.23%), Postives = 213/262 (81.30%), Query Frame = 0

Query: 1   MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK 60
           ME  N+H+  +G           SSSS PPRR ICPNC+RP+PVCLCKFLPS PIAT+TK
Sbjct: 1   MESTNNHNFAAG----------YSSSSRPPRRPICPNCNRPQPVCLCKFLPSSPIATKTK 60

Query: 61  IIILQHPHEARHKLSTTPVLGNCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSA-AS 120
           IIILQHPHE++HKLSTTP+L  CLLNATTIV RRL+PGLS+LLDQSPPAV+LFPP+  +S
Sbjct: 61  IIILQHPHESQHKLSTTPILTKCLLNATTIVNRRLKPGLSKLLDQSPPAVFLFPPTPHSS 120

Query: 121 PAVDISSLDSSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDENVD 180
           P ++IS L+ +        DLVLIAFDGTWKHAKEMVRSSEEFLS+FA RVCLD DE+V+
Sbjct: 121 PPINISDLNIT-------EDLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDIDESVE 180

Query: 181 GGSIYDSELILRKQPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGYLK 240
           GGSI+DSELILRK+PHGGCVSTMEAVARCL  +E +GA +E +LIGVLREMVR QAG+LK
Sbjct: 181 GGSIFDSELILRKEPHGGCVSTMEAVARCLRVMELDGAAVEEKLIGVLREMVRLQAGFLK 240

Query: 241 PMNPRPK-LKNSKNKKKNKENE 261
           PMNPRPK LKN K K    E+E
Sbjct: 241 PMNPRPKLLKNFKQKTSVMEDE 245

BLAST of MS013800 vs. ExPASy TrEMBL
Match: A0A5A7TJW5 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold335G00690 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 3.0e-92
Identity = 184/262 (70.23%), Postives = 213/262 (81.30%), Query Frame = 0

Query: 1   MEPMNHHDNNSGGGGGGGGGGNSSSSSAPPRRSICPNCSRPEPVCLCKFLPSPPIATRTK 60
           ME  N+H+  +G           SSSS PPRR ICPNC+RP+PVCLCKFLPS PIAT+TK
Sbjct: 1   MESTNNHNFAAG----------YSSSSPPPRRPICPNCNRPQPVCLCKFLPSSPIATKTK 60

Query: 61  IIILQHPHEARHKLSTTPVLGNCLLNATTIVGRRLRPGLSELLDQSPPAVYLFPPSA-AS 120
           IIILQHPHE++HKLSTTP+L  CLLNATTIV RRL+PGLS+LLDQSPPAV+LFPP+  +S
Sbjct: 61  IIILQHPHESQHKLSTTPILTKCLLNATTIVNRRLKPGLSKLLDQSPPAVFLFPPTPHSS 120

Query: 121 PAVDISSLDSSSPIRRGDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFATRVCLDSDENVD 180
           P ++IS L+ +        DLVLIAFDGTWKHAKEMVRSSEEFLS+FA RVCLD DE+V+
Sbjct: 121 PPINISDLNIT-------EDLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDIDESVE 180

Query: 181 GGSIYDSELILRKQPHGGCVSTMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGYLK 240
           GGSI+DSELILRK+PHGGCVSTMEAVARCL  +E +GA +E +LIGVLREMVR QAG+LK
Sbjct: 181 GGSIFDSELILRKEPHGGCVSTMEAVARCLRVMELDGAAVEEKLIGVLREMVRLQAGFLK 240

Query: 241 PMNPRPK-LKNSKNKKKNKENE 261
           PMNPRPK LKN K K    E+E
Sbjct: 241 PMNPRPKLLKNFKQKTSVMEDE 245

BLAST of MS013800 vs. TAIR 10
Match: AT2G41750.1 (DTW domain-containing protein )

HSP 1 Score: 239.2 bits (609), Expect = 3.8e-63
Identity = 136/243 (55.97%), Postives = 168/243 (69.14%), Query Frame = 0

Query: 31  RRSICPNCSRPEPVCLCKFLPSPPIATRTKIIILQHPHEARHKLSTTPVLGNCLLNATTI 90
           RR IC NC RP  +CLC  LP+  I T T+IIIL HPHE+RHKL+TTP+L   LLN T I
Sbjct: 10  RRQICDNCDRPNAICLCHVLPADLIPTNTEIIILHHPHESRHKLNTTPLLTKSLLNVTRI 69

Query: 91  VGRR-LRPGLSE---LLDQSPPAVYLFPPSAASPAVDISSLDSSSPIRRGD----SDLVL 150
             RR LR  +S        +PP +YLFP S +SPAV IS   S + +   +      L L
Sbjct: 70  PARRLLRRHISTAGGTALPAPPTIYLFPSSPSSPAVTISEFKSLNLLNHREISNPPPLRL 129

Query: 151 IAFDGTWKHAKEMVRSSEEFLSKF-ATRVCLDS--DENVDGGSIYDSELILRKQPHGGCV 210
           I FD TWKHAKEMV++SE  L +  A RVCLD+  D +V GG+IYDSEL+LRK+P GGCV
Sbjct: 130 IVFDATWKHAKEMVKASEVVLREAGAVRVCLDTEIDASVSGGTIYDSELVLRKEPFGGCV 189

Query: 211 STMEAVARCLGAVESNGAEIERRLIGVLREMVRFQAGYLKPMNPRPKL--KNSKNKKKNK 261
           +T EAVARCLGA+E +G EIER+LI VL+EMVRFQ+ YLKPM PRPKL  K  +N++  +
Sbjct: 190 TTAEAVARCLGAIEPDGEEIERKLISVLKEMVRFQSKYLKPMKPRPKLLKKRFQNQQPLE 249

BLAST of MS013800 vs. TAIR 10
Match: AT5G54880.1 (DTW domain-containing protein )

HSP 1 Score: 48.9 bits (115), Expect = 7.2e-06
Identity = 24/62 (38.71%), Postives = 36/62 (58.06%), Query Frame = 0

Query: 31  RRSICPNCSRPEPVCLCKFLPSPPIATRTKIIILQHPHEARHKLSTTPV--LGNCLLNAT 90
           +R  CP+C +P  +CLCK + SP    +  I ILQH  E +H L++T +  LG   +  T
Sbjct: 44  KRPTCPSCDKPSQLCLCKKMRSPCFDNQVSITILQHSLERKHALNSTRIARLGLKNVGVT 103

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152239.17.2e-14198.47DTW domain-containing protein 2 [Momordica charantia][more]
XP_023544253.13.0e-9973.51DTW domain-containing protein 2 [Cucurbita pepo subsp. pepo][more]
XP_022928716.16.8e-9973.13DTW domain-containing protein 2 [Cucurbita moschata][more]
XP_022967888.12.0e-9873.28DTW domain-containing protein 2 [Cucurbita maxima][more]
KAG7033522.11.7e-9772.39DTW domain-containing protein 2, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
Match NameE-valueIdentityDescription
Q8NBA81.7e-1530.08tRNA-uridine aminocarboxypropyltransferase 2 OS=Homo sapiens OX=9606 GN=DTWD2 PE... [more]
Q4R7M41.7e-1530.08tRNA-uridine aminocarboxypropyltransferase 2 OS=Macaca fascicularis OX=9541 GN=D... [more]
Q9D0U11.2e-1328.09tRNA-uridine aminocarboxypropyltransferase 2 OS=Mus musculus OX=10090 GN=Dtwd2 P... [more]
Match NameE-valueIdentityDescription
A0A6J1DFG53.5e-14198.47tRNA-uridine aminocarboxypropyltransferase OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1EL333.3e-9973.13tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1HWG29.6e-9973.28tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5D3D8833.0e-9270.23tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=119469... [more]
A0A5A7TJW53.0e-9270.23tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=119469... [more]
Match NameE-valueIdentityDescription
AT2G41750.13.8e-6355.97DTW domain-containing protein [more]
AT5G54880.17.2e-0638.71DTW domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005636DTWSMARTSM01144DTW_2acoord: 31..239
e-value: 2.0E-44
score: 163.6
IPR005636DTWPFAMPF03942DTWcoord: 33..234
e-value: 3.4E-43
score: 147.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 241..262
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..33
NoneNo IPR availablePANTHERPTHR21392:SF0DTW DOMAIN-CONTAINING PROTEIN 2coord: 22..249
IPR039262DTW domain-containing protein DTWD2/YfiPPANTHERPTHR21392UNCHARACTERIZEDcoord: 22..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS013800.1MS013800.1mRNA