MS013616 (gene) Bitter gourd (TR) v1

BLAST of MS013616 vs. NCBI nr
Match: XP_022139821.1 (CBS domain-containing protein CBSX5-like [Momordica charantia])

HSP 1 Score: 754.6 bits (1947), Expect = 4.3e-214
Identity = 390/391 (99.74%), Postives = 391/391 (100.00%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSSKANSEL 60
           MAVSLLARE+SDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSSKANSEL
Sbjct: 1   MAVSLLAREVSDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSSKANSEL 60

Query: 61  HCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAIDL 120
           HCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAIDL
Sbjct: 61  HCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAIDL 120

Query: 121 ILDGAHNLVIPIKSSTSKRKNFLKKASSISSLHNDREYCWLAPEDIIRYLLNSIGLFSHA 180
           ILDGAHNLVIPIKSSTSKRKNFLKKASSISSLHNDREYCWLAPEDIIRYLLNSIGLFSHA
Sbjct: 121 ILDGAHNLVIPIKSSTSKRKNFLKKASSISSLHNDREYCWLAPEDIIRYLLNSIGLFSHA 180

Query: 181 AASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISPFTL 240
           AASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISPFTL
Sbjct: 181 AASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISPFTL 240

Query: 241 NFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESSTTS 300
           NFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESSTTS
Sbjct: 241 NFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESSTTS 300

Query: 301 SSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQALAHRVGY 360
           SSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQALAHRVGY
Sbjct: 301 SSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQALAHRVGY 360

Query: 361 VWVIEEDGGLAGIVTFASMLSVFRDRLKSIG 392
           VWVIEEDGGLAGIVTFASMLSVFRDRLKSIG
Sbjct: 361 VWVIEEDGGLAGIVTFASMLSVFRDRLKSIG 391

BLAST of MS013616 vs. NCBI nr
Match: XP_038893760.1 (CBS domain-containing protein CBSX5-like [Benincasa hispida])

HSP 1 Score: 652.9 bits (1683), Expect = 1.7e-183
Identity = 349/397 (87.91%), Postives = 365/397 (91.94%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSS-KANSE 60
           MA SLLA E+SDLCLGKPALRSISISATVADALSVLTRIDEGYIS+WSCGDHSS K + +
Sbjct: 1   MAASLLACEVSDLCLGKPALRSISISATVADALSVLTRIDEGYISIWSCGDHSSLKPDFD 60

Query: 61  LHCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAID 120
           LHCRCVGKVCMVDIICFLCRQENLLQPA ALRSPIS LI E +GLVRHLEPH SLMEAID
Sbjct: 61  LHCRCVGKVCMVDIICFLCRQENLLQPAVALRSPISVLIPEGLGLVRHLEPHVSLMEAID 120

Query: 121 LILDGAHNLVIPIKSSTSKRKNFLKKAS--SISSLHNDREYCWLAPEDIIRYLLNSIGLF 180
           LI +G HNLVIPIK STSKRK+FLKK+S  SISSLHND+EYCWLAPEDIIRYLLNSIGLF
Sbjct: 121 LIHEGVHNLVIPIKMSTSKRKSFLKKSSSNSISSLHNDQEYCWLAPEDIIRYLLNSIGLF 180

Query: 181 SHAAASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISP 240
           S  AA+PI+SFNIIDT N+LAVHYDESALSILPLISQALIHQSSVAIVDLDD LIGEISP
Sbjct: 181 SPIAANPIDSFNIIDTINILAVHYDESALSILPLISQALIHQSSVAIVDLDDKLIGEISP 240

Query: 241 FTLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESS 300
           FTLNFCDETV AAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEW EEESS
Sbjct: 241 FTLNFCDETVAAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWFEEESS 300

Query: 301 TTSSSSSSICSSSDDEFGS----GSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQA 360
           T  SSSSSICSSSDDEFGS    GSGRSGK+GGYSARV RRSEAIVCYPWNSLVAVMIQA
Sbjct: 301 TI-SSSSSICSSSDDEFGSGSSCGSGRSGKMGGYSARVLRRSEAIVCYPWNSLVAVMIQA 360

Query: 361 LAHRVGYVWVIEEDGGLAGIVTFASMLSVFRDRLKSI 391
           LAHRV YVWV +EDG LAG VTFASML+VFRDRLKS+
Sbjct: 361 LAHRVSYVWVTQEDGTLAGTVTFASMLAVFRDRLKSL 396

BLAST of MS013616 vs. NCBI nr
Match: XP_022954486.1 (CBS domain-containing protein CBSX5-like [Cucurbita moschata] >KAG7012426.1 CBS domain-containing protein CBSX5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 648.3 bits (1671), Expect = 4.3e-182
Identity = 341/393 (86.77%), Postives = 363/393 (92.37%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSSKANSEL 60
           MAVSLL+ +ISDLCLGKPA+RSISISATVADALSVLTRIDEG ISVWSCGDHSS+A+S+L
Sbjct: 1   MAVSLLSHDISDLCLGKPAIRSISISATVADALSVLTRIDEGCISVWSCGDHSSEADSDL 60

Query: 61  HCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAIDL 120
           HCRCVGKVCMVDIICFLCRQENLLQPA ALRSPIS LI E + LVRHLEPH+SLMEAIDL
Sbjct: 61  HCRCVGKVCMVDIICFLCRQENLLQPAIALRSPISVLIPEGLELVRHLEPHASLMEAIDL 120

Query: 121 ILDGAHNLVIPIKSSTSKRKNFLKKA--SSISSLHNDREYCWLAPEDIIRYLLNSIGLFS 180
           I DG HNLVIPI  S SKR+NFL+ A  +SISSLHNDR+YCWLAPEDIIRYLLNSIGLFS
Sbjct: 121 IRDGVHNLVIPINMSASKRRNFLETATSNSISSLHNDRQYCWLAPEDIIRYLLNSIGLFS 180

Query: 181 HAAASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISPF 240
             AASPINS NII+TNN+L VHYD+SALSILPLISQALIHQSSVAIVDLDD L+GEISPF
Sbjct: 181 PTAASPINSVNIIETNNILTVHYDDSALSILPLISQALIHQSSVAIVDLDDKLVGEISPF 240

Query: 241 TLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESST 300
           TLNFCDET+VAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLE+VEEESS 
Sbjct: 241 TLNFCDETLVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEFVEEESSP 300

Query: 301 TSSSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQALAHRV 360
             SSSSSICS SDDEFG GSGRS KIGGYSARV RRSEAIVC+PWNSLVAVMIQALAHRV
Sbjct: 301 I-SSSSSICSLSDDEFGCGSGRSWKIGGYSARVLRRSEAIVCHPWNSLVAVMIQALAHRV 360

Query: 361 GYVWVIEEDGGLAGIVTFASMLSVFRDRLKSIG 392
            YVWVI+EDG LAG VTFASML+VFRDRLKS+G
Sbjct: 361 SYVWVIQEDGTLAGTVTFASMLAVFRDRLKSVG 392

BLAST of MS013616 vs. NCBI nr
Match: XP_004146648.1 (CBS domain-containing protein CBSX5 [Cucumis sativus] >KGN64667.1 hypothetical protein Csa_013582 [Cucumis sativus])

HSP 1 Score: 647.5 bits (1669), Expect = 7.3e-182
Identity = 344/396 (86.87%), Postives = 361/396 (91.16%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDH-SSKANSE 60
           MA SLLA E+SDLCLGKPALRSISISAT+ADALS+LT+IDEGYISVWSCGDH SSKA+S+
Sbjct: 1   MAASLLACEVSDLCLGKPALRSISISATLADALSILTKIDEGYISVWSCGDHSSSKADSD 60

Query: 61  LHCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAID 120
           LHCRCVGKVCMVDIICFLCRQENLLQPA  L+SPIS LI E   LVRHLEPH+SLMEAID
Sbjct: 61  LHCRCVGKVCMVDIICFLCRQENLLQPAIGLQSPISVLIPEGFELVRHLEPHASLMEAID 120

Query: 121 LILDGAHNLVIPIKSSTSKRKNFLKK--ASSISSLHNDREYCWLAPEDIIRYLLNSIGLF 180
           LI DG HNLVIPIK S SKRKN LKK  A+SISSLHND+EYCWLAPEDIIRYLLNSIGLF
Sbjct: 121 LIHDGVHNLVIPIKMSISKRKNILKKSLANSISSLHNDQEYCWLAPEDIIRYLLNSIGLF 180

Query: 181 SHAAASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISP 240
           S  AA+PINSFNIIDTNN+LAV YDESALSILPLISQALIHQSSVAIVDLDD LIGEISP
Sbjct: 181 STTAANPINSFNIIDTNNILAVRYDESALSILPLISQALIHQSSVAIVDLDDKLIGEISP 240

Query: 241 FTLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESS 300
           FTLNFCDETVVAAIATLTAGELM YIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEES 
Sbjct: 241 FTLNFCDETVVAAIATLTAGELMGYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESL 300

Query: 301 TTSSSSSSICSSSDDEFG-----SGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQ 360
           T SSSSSSICSSSDDEFG     SGSGRSG+I GYSARV RRSEAIVCYPWNSLVAVMIQ
Sbjct: 301 TISSSSSSICSSSDDEFGCGSSSSGSGRSGRICGYSARVMRRSEAIVCYPWNSLVAVMIQ 360

Query: 361 ALAHRVGYVWVIEEDGGLAGIVTFASMLSVFRDRLK 389
           ALAHRV Y+WVI+EDG LAG VTF S+L+VFRDRLK
Sbjct: 361 ALAHRVSYMWVIQEDGTLAGTVTFPSLLAVFRDRLK 396

BLAST of MS013616 vs. NCBI nr
Match: XP_022994407.1 (CBS domain-containing protein CBSX5-like [Cucurbita maxima])

HSP 1 Score: 643.7 bits (1659), Expect = 1.1e-180
Identity = 339/393 (86.26%), Postives = 360/393 (91.60%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSSKANSEL 60
           MAVSLL+ +ISDLCLGKPA+RSISISATVADALSVLTRIDEG ISVWSCGDHSS+A S+L
Sbjct: 1   MAVSLLSHDISDLCLGKPAIRSISISATVADALSVLTRIDEGCISVWSCGDHSSEAESDL 60

Query: 61  HCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAIDL 120
           HCRCVGKVCMVDIICFLCRQENLLQPA ALRSPIS LI E + LVRHLEPH+SLMEAIDL
Sbjct: 61  HCRCVGKVCMVDIICFLCRQENLLQPAIALRSPISVLIPEGLELVRHLEPHASLMEAIDL 120

Query: 121 ILDGAHNLVIPIKSSTSKRKNFLKKAS--SISSLHNDREYCWLAPEDIIRYLLNSIGLFS 180
           I DG HNLVIPI  S SKR+NFL+ A+  SISSLHNDR+YCWLAPEDIIRYLLNSIGLFS
Sbjct: 121 IRDGVHNLVIPINMSASKRRNFLETATSKSISSLHNDRQYCWLAPEDIIRYLLNSIGLFS 180

Query: 181 HAAASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISPF 240
             AASPINS NII+TNN+L VHYD+SALSILPLISQALIHQSSVAIVDLDD L+GEISPF
Sbjct: 181 PTAASPINSVNIIETNNILTVHYDDSALSILPLISQALIHQSSVAIVDLDDKLVGEISPF 240

Query: 241 TLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESST 300
           TLNFCDET+VAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLE+VEEESS 
Sbjct: 241 TLNFCDETLVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEFVEEESSP 300

Query: 301 TSSSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQALAHRV 360
             SSSSSICS SDDEFG GSGRS KIGGYSARV RRSEAIVC+PWNSLVAVMIQALAHRV
Sbjct: 301 I-SSSSSICSLSDDEFGCGSGRSWKIGGYSARVLRRSEAIVCHPWNSLVAVMIQALAHRV 360

Query: 361 GYVWVIEEDGGLAGIVTFASMLSVFRDRLKSIG 392
            YVWVI+EDG LAG VTF SML+VFRD LKS+G
Sbjct: 361 SYVWVIQEDGTLAGTVTFGSMLAVFRDGLKSVG 392

BLAST of MS013616 vs. ExPASy Swiss-Prot
Match: Q84WQ5 (CBS domain-containing protein CBSX5 OS=Arabidopsis thaliana OX=3702 GN=CBSX5 PE=2 SV=2)

HSP 1 Score: 295.0 bits (754), Expect = 1.2e-78
Identity = 176/398 (44.22%), Postives = 258/398 (64.82%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSI-SISATVADALSVLTRIDEGYISVWSCGDHSSKANSE 60
           MA+SLL+  +SDLCLGKP LR + S S++V+DA++ L   ++ ++SVW+C +H    N+E
Sbjct: 1   MALSLLSYNVSDLCLGKPPLRCLSSSSSSVSDAIAALKSSEDTFLSVWNC-NHDDDNNTE 60

Query: 61  LHCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAID 120
             C C+GK+ M D+IC L +  +      AL S +S L+ +   +V H++P  SL+EAID
Sbjct: 61  --CECLGKISMADVICHLSKDHD--HSLCALNSSVSVLLPKTRSIVLHVQPSCSLIEAID 120

Query: 121 LILDGAHNLVIPIKSST-SKRKNFLKKASSISSLH-NDREYCWLAPEDIIRYLLNSIGLF 180
           LI+ GA NL++PI +   +K+K      S  ++ H N + +CW+  EDII++LL  I  F
Sbjct: 121 LIIKGAQNLIVPIHTKPYTKKKQHNDNVSVTTTTHSNGQRFCWITQEDIIQFLLGFIAAF 180

Query: 181 SHAAASPINSFNIID-TNNVLAVHYDESALSILPLISQALIHQSSVAIVDLD-----DNL 240
           S   A  ++   +I+ T+ V+AV Y  SA +++  +S AL  Q+SVA+VD +      +L
Sbjct: 181 SPLPAMSLSDLGVINSTHTVVAVDYHSSASAVVSAVSNALAVQTSVAVVDGEGDDPFTSL 240

Query: 241 IGEISPFTLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEW 300
           IGEISP TL  CDET  AA+ATL+AG+LMAYID   PP+ LVQ+V+ RLE+K L  ++  
Sbjct: 241 IGEISPMTLTCCDETAAAAVATLSAGDLMAYIDGANPPESLVQIVRNRLEDKGLIGLMSL 300

Query: 301 VEEESSTTSSSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMI 360
            +  SS ++SS      SS++E    +   G+    SAR+ R+SEAIVC P +SL+AVMI
Sbjct: 301 FDSLSSYSTSSG----YSSEEEAPVRTTSYGRSMSSSARMARKSEAIVCNPKSSLMAVMI 360

Query: 361 QALAHRVGYVWVIEEDGGLAGIVTFASMLSVFRDRLKS 390
           QA+AHRV Y WV+E+DG   G+VTF  +L VFR  L++
Sbjct: 361 QAVAHRVNYAWVVEKDGCFVGMVTFVDILKVFRKFLEN 389

BLAST of MS013616 vs. ExPASy Swiss-Prot
Match: Q8GZA4 (CBS domain-containing protein CBSX6 OS=Arabidopsis thaliana OX=3702 GN=CBSX6 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.0e-20
Identity = 116/422 (27.49%), Postives = 193/422 (45.73%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSS----KA 60
           MA   L   + DL +GKP +     + TV  A+  +    E  I VW      S      
Sbjct: 1   MASVFLYHVVGDLTVGKPEMVEFYETETVESAIRAIGESTECGIPVWRKRTTPSLPGFVE 60

Query: 61  NSEL-HCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLM 120
           NSE+   R VG +  +DI+ FL + E  LQ   A++ P+S ++  D  L++ ++P + L+
Sbjct: 61  NSEMRQQRFVGILNSLDIVAFLAKTE-CLQEEKAMKIPVSEVVSPDNTLLKQVDPGTRLI 120

Query: 121 EAIDLILDGAHNLVIP---IKSSTSKRKNFL---------KKASSISSLHNDR------- 180
           +A++++  G   L++P   +    SKR + L         + +SS S L  D        
Sbjct: 121 DALEMMKQGVRRLLVPKSVVWRGMSKRFSILYNGKWLKNSENSSSSSGLSADSTNRPTTS 180

Query: 181 ------EYCWLAPEDIIRYLLNSIGLFSHAAASPINSFNIIDTNNVLAVHYDESALSILP 240
                 ++C L+ ED+IR+L+  +G  +    + I++  II+ N     ++ E++L  + 
Sbjct: 181 MTSSRDKFCCLSREDVIRFLIGVLGALAPLPLTSISTLGIINQN----YNFIEASLPAIE 240

Query: 241 LISQALIHQSSVAIVDLDDN-----LIGEISPFTLNFCDETVVA-AIATLTAGELMAYID 300
              + L   S++A+++  +N     +IGEIS   L  CD    A A+A L AG+ +    
Sbjct: 241 ATRRPLCDPSAIAVLEQTENEQQFKIIGEISASKLWKCDYLAAAWALANLYAGQFVM--- 300

Query: 301 CGGPPDDLVQLVKERLEEKNLEAVLEWVEEESSTTSS---SSSSICSSSDDEFGSGSGRS 360
             G  D++      R     L+      E+  + T++   SS SI  +         GRS
Sbjct: 301 --GVEDNM----SSRSFSDFLQTSFPGGEQNGTATNAKKFSSRSIGFNPTSPTRLSIGRS 360

Query: 361 GKIGGYSARVGRRSEAIVCYPWNSLVAVMIQALAHRVGYVWVIEEDGG--LAGIVTFASM 382
              G        RS  + C   +SL AVM Q L+HR  +VWV E D    L G+V +  +
Sbjct: 361 MYRG--------RSAPLTCKTSSSLAAVMAQMLSHRATHVWVTEADSDDVLVGVVGYGEI 400

BLAST of MS013616 vs. ExPASy Swiss-Prot
Match: Q8GXI9 (SNF1-related protein kinase regulatory subunit gamma-like PV42b OS=Arabidopsis thaliana OX=3702 GN=PV42B PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 6.8e-05
Identity = 84/403 (20.84%), Postives = 163/403 (40.45%), Query Frame = 0

Query: 5   LLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISV------WSCGDHS----- 64
           L+  ++ DL + K  L  +  +AT+ DAL+ +       + V      W     S     
Sbjct: 7   LMQFKVKDLMIDKRRLVEVPDNATLGDALNTMVANRVRAVPVAAKPGQWLGAGGSMIVEL 66

Query: 65  SKANSELHCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLI-HEDVGL-VRHLEPH 124
            K +     + +G V M+D++  +   +        + +P+S +I H   GL +  L P+
Sbjct: 67  DKQSGSARKQYIGMVTMLDVVAHIAGDDGESGLDKKMAAPVSSIIGHCPEGLSLWSLNPN 126

Query: 125 SSLMEAIDLILDGAHNLVIPIKSSTSKRKNFLKKASSISSLHNDREYCWLAPEDIIRYLL 184
           +S+M+ ++++  G H +++P+ S+T       +  +    + +   Y  L+  D+I +  
Sbjct: 127 TSIMDCMEMLSKGIHRVLVPLDSNT-------ENITGPELVESASAYAMLSQMDLISFFF 186

Query: 185 NSI----GLFSHAAASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDL 244
           +      G+ SH               ++ A+H      ++L L SQA +          
Sbjct: 187 DQSSQLHGILSHT------------VTDLSAIHN-----TVLALTSQARVK--------- 246

Query: 245 DDNLIGEISPFTLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEA 304
                           D     +IA L A   +  ++  G  +D  QLV    + KN   
Sbjct: 247 ----------------DAIQCMSIAMLNA---VPIVEASGEGEDHKQLV----DGKNRRV 306

Query: 305 VLEW-VEEESSTTSSSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSL 364
           V  +   +      ++  S    +  EF     R+      ++  GR  E + C+  ++L
Sbjct: 307 VGTFSASDLKGCHLATLRSWLPLNALEFVEKIPRTLLFTAATSTPGR--ELVTCHVTSTL 351

Query: 365 VAVMIQALAHRVGYVWVIEEDGGLAGIVTFASMLSVFRDRLKS 390
             V+      RV  VWV++++GGL G+V+   +++V R  L S
Sbjct: 367 AQVIHMVTTKRVHRVWVVDQNGGLQGLVSLTDIIAVVRSALLS 351

BLAST of MS013616 vs. ExPASy TrEMBL
Match: A0A6J1CDV2 (CBS domain-containing protein CBSX5-like OS=Momordica charantia OX=3673 GN=LOC111010644 PE=4 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 2.1e-214
Identity = 390/391 (99.74%), Postives = 391/391 (100.00%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSSKANSEL 60
           MAVSLLARE+SDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSSKANSEL
Sbjct: 1   MAVSLLAREVSDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSSKANSEL 60

Query: 61  HCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAIDL 120
           HCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAIDL
Sbjct: 61  HCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAIDL 120

Query: 121 ILDGAHNLVIPIKSSTSKRKNFLKKASSISSLHNDREYCWLAPEDIIRYLLNSIGLFSHA 180
           ILDGAHNLVIPIKSSTSKRKNFLKKASSISSLHNDREYCWLAPEDIIRYLLNSIGLFSHA
Sbjct: 121 ILDGAHNLVIPIKSSTSKRKNFLKKASSISSLHNDREYCWLAPEDIIRYLLNSIGLFSHA 180

Query: 181 AASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISPFTL 240
           AASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISPFTL
Sbjct: 181 AASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISPFTL 240

Query: 241 NFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESSTTS 300
           NFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESSTTS
Sbjct: 241 NFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESSTTS 300

Query: 301 SSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQALAHRVGY 360
           SSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQALAHRVGY
Sbjct: 301 SSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQALAHRVGY 360

Query: 361 VWVIEEDGGLAGIVTFASMLSVFRDRLKSIG 392
           VWVIEEDGGLAGIVTFASMLSVFRDRLKSIG
Sbjct: 361 VWVIEEDGGLAGIVTFASMLSVFRDRLKSIG 391

BLAST of MS013616 vs. ExPASy TrEMBL
Match: A0A6J1GR12 (CBS domain-containing protein CBSX5-like OS=Cucurbita moschata OX=3662 GN=LOC111456738 PE=4 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 2.1e-182
Identity = 341/393 (86.77%), Postives = 363/393 (92.37%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSSKANSEL 60
           MAVSLL+ +ISDLCLGKPA+RSISISATVADALSVLTRIDEG ISVWSCGDHSS+A+S+L
Sbjct: 1   MAVSLLSHDISDLCLGKPAIRSISISATVADALSVLTRIDEGCISVWSCGDHSSEADSDL 60

Query: 61  HCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAIDL 120
           HCRCVGKVCMVDIICFLCRQENLLQPA ALRSPIS LI E + LVRHLEPH+SLMEAIDL
Sbjct: 61  HCRCVGKVCMVDIICFLCRQENLLQPAIALRSPISVLIPEGLELVRHLEPHASLMEAIDL 120

Query: 121 ILDGAHNLVIPIKSSTSKRKNFLKKA--SSISSLHNDREYCWLAPEDIIRYLLNSIGLFS 180
           I DG HNLVIPI  S SKR+NFL+ A  +SISSLHNDR+YCWLAPEDIIRYLLNSIGLFS
Sbjct: 121 IRDGVHNLVIPINMSASKRRNFLETATSNSISSLHNDRQYCWLAPEDIIRYLLNSIGLFS 180

Query: 181 HAAASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISPF 240
             AASPINS NII+TNN+L VHYD+SALSILPLISQALIHQSSVAIVDLDD L+GEISPF
Sbjct: 181 PTAASPINSVNIIETNNILTVHYDDSALSILPLISQALIHQSSVAIVDLDDKLVGEISPF 240

Query: 241 TLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESST 300
           TLNFCDET+VAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLE+VEEESS 
Sbjct: 241 TLNFCDETLVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEFVEEESSP 300

Query: 301 TSSSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQALAHRV 360
             SSSSSICS SDDEFG GSGRS KIGGYSARV RRSEAIVC+PWNSLVAVMIQALAHRV
Sbjct: 301 I-SSSSSICSLSDDEFGCGSGRSWKIGGYSARVLRRSEAIVCHPWNSLVAVMIQALAHRV 360

Query: 361 GYVWVIEEDGGLAGIVTFASMLSVFRDRLKSIG 392
            YVWVI+EDG LAG VTFASML+VFRDRLKS+G
Sbjct: 361 SYVWVIQEDGTLAGTVTFASMLAVFRDRLKSVG 392

BLAST of MS013616 vs. ExPASy TrEMBL
Match: A0A0A0LV40 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073740 PE=4 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 3.5e-182
Identity = 344/396 (86.87%), Postives = 361/396 (91.16%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDH-SSKANSE 60
           MA SLLA E+SDLCLGKPALRSISISAT+ADALS+LT+IDEGYISVWSCGDH SSKA+S+
Sbjct: 1   MAASLLACEVSDLCLGKPALRSISISATLADALSILTKIDEGYISVWSCGDHSSSKADSD 60

Query: 61  LHCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAID 120
           LHCRCVGKVCMVDIICFLCRQENLLQPA  L+SPIS LI E   LVRHLEPH+SLMEAID
Sbjct: 61  LHCRCVGKVCMVDIICFLCRQENLLQPAIGLQSPISVLIPEGFELVRHLEPHASLMEAID 120

Query: 121 LILDGAHNLVIPIKSSTSKRKNFLKK--ASSISSLHNDREYCWLAPEDIIRYLLNSIGLF 180
           LI DG HNLVIPIK S SKRKN LKK  A+SISSLHND+EYCWLAPEDIIRYLLNSIGLF
Sbjct: 121 LIHDGVHNLVIPIKMSISKRKNILKKSLANSISSLHNDQEYCWLAPEDIIRYLLNSIGLF 180

Query: 181 SHAAASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISP 240
           S  AA+PINSFNIIDTNN+LAV YDESALSILPLISQALIHQSSVAIVDLDD LIGEISP
Sbjct: 181 STTAANPINSFNIIDTNNILAVRYDESALSILPLISQALIHQSSVAIVDLDDKLIGEISP 240

Query: 241 FTLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESS 300
           FTLNFCDETVVAAIATLTAGELM YIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEES 
Sbjct: 241 FTLNFCDETVVAAIATLTAGELMGYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESL 300

Query: 301 TTSSSSSSICSSSDDEFG-----SGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQ 360
           T SSSSSSICSSSDDEFG     SGSGRSG+I GYSARV RRSEAIVCYPWNSLVAVMIQ
Sbjct: 301 TISSSSSSICSSSDDEFGCGSSSSGSGRSGRICGYSARVMRRSEAIVCYPWNSLVAVMIQ 360

Query: 361 ALAHRVGYVWVIEEDGGLAGIVTFASMLSVFRDRLK 389
           ALAHRV Y+WVI+EDG LAG VTF S+L+VFRDRLK
Sbjct: 361 ALAHRVSYMWVIQEDGTLAGTVTFPSLLAVFRDRLK 396

BLAST of MS013616 vs. ExPASy TrEMBL
Match: A0A6J1JVR0 (CBS domain-containing protein CBSX5-like OS=Cucurbita maxima OX=3661 GN=LOC111490134 PE=4 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 5.1e-181
Identity = 339/393 (86.26%), Postives = 360/393 (91.60%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSSKANSEL 60
           MAVSLL+ +ISDLCLGKPA+RSISISATVADALSVLTRIDEG ISVWSCGDHSS+A S+L
Sbjct: 1   MAVSLLSHDISDLCLGKPAIRSISISATVADALSVLTRIDEGCISVWSCGDHSSEAESDL 60

Query: 61  HCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAIDL 120
           HCRCVGKVCMVDIICFLCRQENLLQPA ALRSPIS LI E + LVRHLEPH+SLMEAIDL
Sbjct: 61  HCRCVGKVCMVDIICFLCRQENLLQPAIALRSPISVLIPEGLELVRHLEPHASLMEAIDL 120

Query: 121 ILDGAHNLVIPIKSSTSKRKNFLKKAS--SISSLHNDREYCWLAPEDIIRYLLNSIGLFS 180
           I DG HNLVIPI  S SKR+NFL+ A+  SISSLHNDR+YCWLAPEDIIRYLLNSIGLFS
Sbjct: 121 IRDGVHNLVIPINMSASKRRNFLETATSKSISSLHNDRQYCWLAPEDIIRYLLNSIGLFS 180

Query: 181 HAAASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISPF 240
             AASPINS NII+TNN+L VHYD+SALSILPLISQALIHQSSVAIVDLDD L+GEISPF
Sbjct: 181 PTAASPINSVNIIETNNILTVHYDDSALSILPLISQALIHQSSVAIVDLDDKLVGEISPF 240

Query: 241 TLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESST 300
           TLNFCDET+VAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLE+VEEESS 
Sbjct: 241 TLNFCDETLVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEFVEEESSP 300

Query: 301 TSSSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQALAHRV 360
             SSSSSICS SDDEFG GSGRS KIGGYSARV RRSEAIVC+PWNSLVAVMIQALAHRV
Sbjct: 301 I-SSSSSICSLSDDEFGCGSGRSWKIGGYSARVLRRSEAIVCHPWNSLVAVMIQALAHRV 360

Query: 361 GYVWVIEEDGGLAGIVTFASMLSVFRDRLKSIG 392
            YVWVI+EDG LAG VTF SML+VFRD LKS+G
Sbjct: 361 SYVWVIQEDGTLAGTVTFGSMLAVFRDGLKSVG 392

BLAST of MS013616 vs. ExPASy TrEMBL
Match: A0A5D3DS85 (CBS domain-containing protein CBSX5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00670 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 6.7e-181
Identity = 341/398 (85.68%), Postives = 361/398 (90.70%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDH-SSKANSE 60
           MA +LL  E+SDLCLGKPALRSISISATVADALS+LT+IDEGYISVWSCGDH SSKA+S+
Sbjct: 1   MAATLLGCEVSDLCLGKPALRSISISATVADALSILTKIDEGYISVWSCGDHSSSKADSD 60

Query: 61  LHCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAID 120
           LHCRCVGKVCMVDIICFLCRQENLLQPA  L+SPIS LI E   LV+HLEPH+SLMEAID
Sbjct: 61  LHCRCVGKVCMVDIICFLCRQENLLQPAIGLQSPISVLIPEGFELVKHLEPHASLMEAID 120

Query: 121 LILDGAHNLVIPIKSSTSKRKNFLKK--ASSISSLHNDREYCWLAPEDIIRYLLNSIGLF 180
           LI DG HNLVIPIK STS+RKN LKK  A+SISSLHND+EYCWLAPEDIIRYLLNSIGLF
Sbjct: 121 LIHDGIHNLVIPIKMSTSQRKNILKKSPANSISSLHNDQEYCWLAPEDIIRYLLNSIGLF 180

Query: 181 SHAAASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQSSVAIVDLDDNLIGEISP 240
           S  AA+PINS NIIDTNN+LAV YDESALSILPLISQALIHQSSVAIVDLDD LIGEISP
Sbjct: 181 SSTAANPINSLNIIDTNNILAVRYDESALSILPLISQALIHQSSVAIVDLDDKLIGEISP 240

Query: 241 FTLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESS 300
            TLNFCDETVVAAIATLTAGE MAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEES 
Sbjct: 241 LTLNFCDETVVAAIATLTAGEFMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEWVEEESL 300

Query: 301 TTSSSSSSICSSSDDEFG-----SGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMIQ 360
           T SSSSSSICSSSDDEFG     +GSGRSG+I GYSARV RRSEAIVCYPWNSLVAVMIQ
Sbjct: 301 TISSSSSSICSSSDDEFGCGSSSTGSGRSGRICGYSARVMRRSEAIVCYPWNSLVAVMIQ 360

Query: 361 ALAHRVGYVWVIEEDGGLAGIVTFASMLSVFRDRLKSI 391
           ALAHRV YVWVI+ DG LAG VTFAS+L+VFRDRLKS+
Sbjct: 361 ALAHRVSYVWVIQNDGTLAGTVTFASLLAVFRDRLKSL 398

BLAST of MS013616 vs. TAIR 10
Match: AT5G53750.1 (CBS domain-containing protein )

HSP 1 Score: 307.0 bits (785), Expect = 2.2e-83
Identity = 186/408 (45.59%), Postives = 269/408 (65.93%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISI-SATVADALSVLTRIDEGYISVWSCGDHSSKANSE 60
           MA++LL+ E+SDLC+GKP LR +S+ +ATVADA++ L   DE +++VWSC +H  K +  
Sbjct: 1   MALTLLSHELSDLCIGKPPLRCLSVATATVADAIAALKSSDEPFLTVWSC-NHDEKTDDN 60

Query: 61  LHCRCVGKVCMVDIICFLCR-QENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAI 120
             C C+GK+CM D+IC+L +   N+L  ++A  + +S L+ +   LV H++   SL+EAI
Sbjct: 61  DKCECLGKICMADVICYLSKFDNNVLSLSSAFDASVSVLLPKSRALVVHVQSSCSLIEAI 120

Query: 121 DLILDGAHNLVIPI--KSSTSKR--KNFLKK------ASSISSLH-NDREYCWLAPEDII 180
           DLI+ GA NL++PI  KS T +R  +  LK+       ++ S+ H N RE+CW+  EDII
Sbjct: 121 DLIIKGAQNLIVPIHTKSITKRRQQQKLLKRNVVVSLTNATSTTHKNSREFCWITQEDII 180

Query: 181 RYLLNSIGLFSHAAASPINSFNIID-TNNVLAVHYDESALSILPLISQALIHQSSVAIV- 240
           R+LL+SI +FS   +  I+   +I+ T+ +LAV Y  SA S +  IS+A++   SVA+V 
Sbjct: 181 RFLLDSISVFSPLPSLSISDLGVINSTHTILAVDYYSSAASAVSAISRAILDNVSVAVVG 240

Query: 241 ---DLDDN---LIGEISPFTLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKER 300
              D +D    LIGEISP TL  CDET VAA+ATL+AG+LM+YID  GPP+ LV +V+ R
Sbjct: 241 KGCDQEDPCMVLIGEISPMTLACCDETAVAAVATLSAGDLMSYIDGSGPPESLVGVVRNR 300

Query: 301 LEEKNLEAVLEWVEEESSTTSSSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIV 360
           LE+K +  ++  ++  S ++ SSS     +      S  GRS      +AR+ R+S AIV
Sbjct: 301 LEDKGMVGLISLIDSLSLSSGSSSDEESPAGKTRMTSSYGRS---VSSAARMARKSVAIV 360

Query: 361 CYPWNSLVAVMIQALAHRVGYVWVIEEDGGLAGIVTFASMLSVFRDRL 388
           C   +SL+AVMIQA+AHRV YVWVI+EDG L G+VTF  +L +FR+ L
Sbjct: 361 CNRKSSLMAVMIQAIAHRVSYVWVIDEDGCLIGMVTFVDILKLFREFL 404

BLAST of MS013616 vs. TAIR 10
Match: AT4G27460.1 (Cystathionine beta-synthase (CBS) family protein )

HSP 1 Score: 295.0 bits (754), Expect = 8.6e-80
Identity = 176/398 (44.22%), Postives = 258/398 (64.82%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSI-SISATVADALSVLTRIDEGYISVWSCGDHSSKANSE 60
           MA+SLL+  +SDLCLGKP LR + S S++V+DA++ L   ++ ++SVW+C +H    N+E
Sbjct: 1   MALSLLSYNVSDLCLGKPPLRCLSSSSSSVSDAIAALKSSEDTFLSVWNC-NHDDDNNTE 60

Query: 61  LHCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLMEAID 120
             C C+GK+ M D+IC L +  +      AL S +S L+ +   +V H++P  SL+EAID
Sbjct: 61  --CECLGKISMADVICHLSKDHD--HSLCALNSSVSVLLPKTRSIVLHVQPSCSLIEAID 120

Query: 121 LILDGAHNLVIPIKSST-SKRKNFLKKASSISSLH-NDREYCWLAPEDIIRYLLNSIGLF 180
           LI+ GA NL++PI +   +K+K      S  ++ H N + +CW+  EDII++LL  I  F
Sbjct: 121 LIIKGAQNLIVPIHTKPYTKKKQHNDNVSVTTTTHSNGQRFCWITQEDIIQFLLGFIAAF 180

Query: 181 SHAAASPINSFNIID-TNNVLAVHYDESALSILPLISQALIHQSSVAIVDLD-----DNL 240
           S   A  ++   +I+ T+ V+AV Y  SA +++  +S AL  Q+SVA+VD +      +L
Sbjct: 181 SPLPAMSLSDLGVINSTHTVVAVDYHSSASAVVSAVSNALAVQTSVAVVDGEGDDPFTSL 240

Query: 241 IGEISPFTLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERLEEKNLEAVLEW 300
           IGEISP TL  CDET  AA+ATL+AG+LMAYID   PP+ LVQ+V+ RLE+K L  ++  
Sbjct: 241 IGEISPMTLTCCDETAAAAVATLSAGDLMAYIDGANPPESLVQIVRNRLEDKGLIGLMSL 300

Query: 301 VEEESSTTSSSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIVCYPWNSLVAVMI 360
            +  SS ++SS      SS++E    +   G+    SAR+ R+SEAIVC P +SL+AVMI
Sbjct: 301 FDSLSSYSTSSG----YSSEEEAPVRTTSYGRSMSSSARMARKSEAIVCNPKSSLMAVMI 360

Query: 361 QALAHRVGYVWVIEEDGGLAGIVTFASMLSVFRDRLKS 390
           QA+AHRV Y WV+E+DG   G+VTF  +L VFR  L++
Sbjct: 361 QAVAHRVNYAWVVEKDGCFVGMVTFVDILKVFRKFLEN 389

BLAST of MS013616 vs. TAIR 10
Match: AT1G65320.1 (Cystathionine beta-synthase (CBS) family protein )

HSP 1 Score: 101.7 bits (252), Expect = 1.4e-21
Identity = 116/422 (27.49%), Postives = 193/422 (45.73%), Query Frame = 0

Query: 1   MAVSLLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYISVWSCGDHSS----KA 60
           MA   L   + DL +GKP +     + TV  A+  +    E  I VW      S      
Sbjct: 1   MASVFLYHVVGDLTVGKPEMVEFYETETVESAIRAIGESTECGIPVWRKRTTPSLPGFVE 60

Query: 61  NSEL-HCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLIHEDVGLVRHLEPHSSLM 120
           NSE+   R VG +  +DI+ FL + E  LQ   A++ P+S ++  D  L++ ++P + L+
Sbjct: 61  NSEMRQQRFVGILNSLDIVAFLAKTE-CLQEEKAMKIPVSEVVSPDNTLLKQVDPGTRLI 120

Query: 121 EAIDLILDGAHNLVIP---IKSSTSKRKNFL---------KKASSISSLHNDR------- 180
           +A++++  G   L++P   +    SKR + L         + +SS S L  D        
Sbjct: 121 DALEMMKQGVRRLLVPKSVVWRGMSKRFSILYNGKWLKNSENSSSSSGLSADSTNRPTTS 180

Query: 181 ------EYCWLAPEDIIRYLLNSIGLFSHAAASPINSFNIIDTNNVLAVHYDESALSILP 240
                 ++C L+ ED+IR+L+  +G  +    + I++  II+ N     ++ E++L  + 
Sbjct: 181 MTSSRDKFCCLSREDVIRFLIGVLGALAPLPLTSISTLGIINQN----YNFIEASLPAIE 240

Query: 241 LISQALIHQSSVAIVDLDDN-----LIGEISPFTLNFCDETVVA-AIATLTAGELMAYID 300
              + L   S++A+++  +N     +IGEIS   L  CD    A A+A L AG+ +    
Sbjct: 241 ATRRPLCDPSAIAVLEQTENEQQFKIIGEISASKLWKCDYLAAAWALANLYAGQFVM--- 300

Query: 301 CGGPPDDLVQLVKERLEEKNLEAVLEWVEEESSTTSS---SSSSICSSSDDEFGSGSGRS 360
             G  D++      R     L+      E+  + T++   SS SI  +         GRS
Sbjct: 301 --GVEDNM----SSRSFSDFLQTSFPGGEQNGTATNAKKFSSRSIGFNPTSPTRLSIGRS 360

Query: 361 GKIGGYSARVGRRSEAIVCYPWNSLVAVMIQALAHRVGYVWVIEEDGG--LAGIVTFASM 382
              G        RS  + C   +SL AVM Q L+HR  +VWV E D    L G+V +  +
Sbjct: 361 MYRG--------RSAPLTCKTSSSLAAVMAQMLSHRATHVWVTEADSDDVLVGVVGYGEI 400

BLAST of MS013616 vs. TAIR 10
Match: AT1G80090.1 (Cystathionine beta-synthase (CBS) family protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.4e-05
Identity = 84/410 (20.49%), Postives = 165/410 (40.24%), Query Frame = 0

Query: 5   LLAREISDLCLGKPALRSISISATVADALSVLTRIDEGYIS-------------VWSCGD 64
           L+  ++ DL + K  L  +  +AT+ DAL+ +T +    ++              W    
Sbjct: 45  LMQFKVKDLMIDKRRLVEVPDNATLGDALNTMTILGIKPVANRVRAVPVAAKPGQWLGAG 104

Query: 65  HS-----SKANSELHCRCVGKVCMVDIICFLCRQENLLQPATALRSPISGLI-HEDVGL- 124
            S      K +     + +G V M+D++  +   +        + +P+S +I H   GL 
Sbjct: 105 GSMIVELDKQSGSARKQYIGMVTMLDVVAHIAGDDGESGLDKKMAAPVSSIIGHCPEGLS 164

Query: 125 VRHLEPHSSLMEAIDLILDGAHNLVIPIKSSTSKRKNFLKKASSISSLHNDREYCWLAPE 184
           +  L P++S+M+ ++++  G H +++P+ S+T       +  +    + +   Y  L+  
Sbjct: 165 LWSLNPNTSIMDCMEMLSKGIHRVLVPLDSNT-------ENITGPELVESASAYAMLSQM 224

Query: 185 DIIRYLLNSI----GLFSHAAASPINSFNIIDTNNVLAVHYDESALSILPLISQALIHQS 244
           D+I +  +      G+ SH               ++ A+H      ++L L SQA +   
Sbjct: 225 DLISFFFDQSSQLHGILSHT------------VTDLSAIHN-----TVLALTSQARVK-- 284

Query: 245 SVAIVDLDDNLIGEISPFTLNFCDETVVAAIATLTAGELMAYIDCGGPPDDLVQLVKERL 304
                                  D     +IA L A   +  ++  G  +D  QLV    
Sbjct: 285 -----------------------DAIQCMSIAMLNA---VPIVEASGEGEDHKQLV---- 344

Query: 305 EEKNLEAVLEW-VEEESSTTSSSSSSICSSSDDEFGSGSGRSGKIGGYSARVGRRSEAIV 364
           + KN   V  +   +      ++  S    +  EF     R+      ++  GR  E + 
Sbjct: 345 DGKNRRVVGTFSASDLKGCHLATLRSWLPLNALEFVEKIPRTLLFTAATSTPGR--ELVT 396

Query: 365 CYPWNSLVAVMIQALAHRVGYVWVIEEDGGLAGIVTFASMLSVFRDRLKS 390
           C+  ++L  V+      RV  VWV++++GGL G+V+   +++V R  L S
Sbjct: 405 CHVTSTLAQVIHMVTTKRVHRVWVVDQNGGLQGLVSLTDIIAVVRSALLS 396