MS013392 (gene) Bitter gourd (TR) v1

Overview
NameMS013392
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionWRKY domain-containing protein
Locationscaffold402: 923308 .. 926757 (-)
RNA-Seq ExpressionMS013392
SyntenyMS013392
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTTGCAGTTTGTTTGAGATGGAGAATTATCAAGCTGGTGATTTGACCGATGTGATTCGAGCGACCTCCGCCGCCGGGGGAGGCGGCGGTTACGGTGGCGCTGTGTCCTCGGGTTTTTCTCTCGCCGGCGACCGTCTCTGGCCGGATCTTCCCGCCGACGATTCCGTGAATTTCGGAGATCCGTTATTGTTTCCGACCACTACCCAAGATCCATTTCTTCACCACTATACTTCCACCGTCAATTTTGCTTCTGACGGTGGTGGGAGTTTGGCTGCGGTGGCGGAACGGCCGTGTAATATATTTTCACGTGTGTTGCAGATCTCTCCGAGCTCGAAAGTGGGTGGTGGTGTAATTAATACTACGAGTGAGACGGTGGCGGCTGCGGCGGGGAATTCGGCTGAAGCATCGGCTTGCGGCGGTGGCGCCGCCTCCTCCGCCGCCGTAGTTTCGAACCCACTGGAAAACTCCGGGCTGCAGATCTCTCCTCCACGAAATCCGGCCATTAAAAGAAGGTAAGAAGAACATTAATGATTTAGATTATTTTTACTTAATTTTCTTCTAATTTCATTAAAGATCAATTCAAGCTTGCTCTCGAGGCATTTTTGTTCCTTTTATTTGGTGGGTTTCAAAAAAAATTCAAATTTTTTTGGTGCTTGAATTTTCCTATAAAATTTTATGATTTTTTGGTGAAAGATTGAAGTCCAATTAAAACTATGCTTATCGGTAGAGAGAATTTCAAAACTAATGGAGGCCCTTTCGGGAAATCCCACCATAAAAATTATGGGTTTTAGAAAACATAATTTCCTCCCCTTCTTTAGAGTCATTTTAAGAATATAGTAAAATTATTGGAAGGGACAGTTCGAGCTTATGATCTTAAAATACCATTTTTCTAACATTTTGATATTTTTCGAACTTTATACACAAATACGAAAACTCGATTGTTGATCAAGTCTAATCCTAGTTAGATTGAACTTCCAAGTTTATTCTACATAAATTAGCATAATCTTGTACTGTGATAATTAGAAATAATATGGTCTTCTTTTTATTAAGTAGAGTAAACTAAGAGTTGGTAACATTTATGGTTATTGTTCGTTGTTTAAAAAAAAATAGAAATATTTGACAACTATTTATGTTTCTTATTCCTCATTTGTTTTAGAAGATTTTTAAAATGGTATAGAAATTTTAGAAGTAATAAGTTTCTTGTTTCTTGTTCTCGTTTTTAATTTGTTCTAACATAAATAAAAATCTTATTTTTTCATTATTGAATTTATATAGTGTTGCGACCATGCAATTTTATTTTAAAGGGACGAAACAAAAACAAGAAGCGAAAACGTTATCAAATTGACCTTAAAATTTTTTACTTCTTCGTTTAGACATTATCTTACTTTTAAAACACACCCTCCAAATGATTACGAATAATCATAAAACAGATTATGAAACAAAGAGAAATCGTCACACTAGACAAAATAATCATCGAACCGAACCAAACCGAAAGTTAAATAAGTTTTCATGAATTAATGTTTGGTCGGGGTTTGTAGGAAGAGCCAGGTGAAGAAGGTGGTGTGCATTCCGGCGCCGGCGCCGGCGAACAGCCGGTCAAGCAGCGGCGAAGTGGTTCCTTCTGATCTATGGGCATGGAGAAAGTACGGACAAAAACCCATCAAAGGATCCCCATATCCAAGGTCAAATATATATATTTTTTAATTTTTTTTAAGTTGTTATTAATTTCATGTTATAATTTTCAGTTAAATTATTTTTCCTTAAATAGGAAAGAGAAAAAAAAACTTTTGTTTTCCAGAAACCTGCAAAATATTGCTGCAAGTTTCTGTACGTCTCTCCCTATATATATATGACAAGAAATTGGCACCAATAATTTCATGAATCTCACTCATTTACTCATCAGTTTATTGATTTGAGAGAACTAATAATAAAATTGTCAACTTTGATATTTTTTTTAGAAAATAATTATATATAGAATCTTAGTTAGTTTTGATTTGAACAGGATTTTGTTTTAGAACCTAGGTATGTTTTATCTCTAGAAAATACTTACCTTCGTTCCGATTATCATTAAATGTTTAACAATTAAGAAAGATATTAAGTAATAAATTGTCAGTTTTTTAATGATTGTCGGGATGGAAATAGGTGTTGAGGGTTAAGAAATTAAATAGTTTCTTTTTCATTTTTATGATTGATTATCATCGAATCATCAAAATGATCGGTTGATTTGAGGTTTAGAATGGACTTTATTTGTAAAAAGAATTTATTAGGGATAATGTTTTGTTTTTAGTTATCATTAGTTTTTAAATGCTACTATTGTTTAGTTGCCATATTTATTTGGTTAAGAATGATTCTAACTATATATATCTTTTAATTTTAGTTAGAATTTTAAAGTTGTGTTTTTAGCTGGTGCAGATTTAAAAAAATATAATTTTTCAAAACAAACGGATCTTAAATACATGCATAATCAAAATTTTAATATATTTTTCATGCTATTATAATTTTTTTTTTTGAACAAAAACTAGGAATAAAGATAAAAAGATTTGAACTTAGGTAATTGCTCCAAACGATCTTTATTCTTGGTTACTATTTTGTCCTTACAATCTTGTTATAAACTCCAAGCTGATCATGTATTTTCATTTTAATTTTCATCTTATTATATACTTCTATATTTATGCTTGTACCTAAACAAACCTATGATATATATATATATATATATATATACATACACATTTTATCTTTACGCGTTATAAATATTTGAAGAATCAATTCATAATATATATAAGATCAATATTAACACTTCTCAAAACCCTTATCTTATGTTCCCTATCTTCTTCCATCTTCAGGGGTTATTATCGGTGCAGCAGCTCCAAGGGCTGCTCTGCCCGAAAACAGGTGGAGCGCAGCCGCACCAACCCCAACATGCTTGTCATCACCTACACCTCGGAGCACAACCACCCATGGCCAACTCAACGCAACGCTCTCGCTGGCTCCACTCGAACCCACTCCTCAAGAAACCCCGCCCCTGCCCATAAAACAAGCCCGAAACACGAGCACAACAACGACAATCTAACCTCCCCATTAACGGGGGCCGTAAAGGGGGAGGAGGTGGAAGAAGACCAGACAACAGAGAAAGCAGCAACAACAGCACCAACAACAATAGATAATGATCATCAAGATCGGGAGGGAGATCATCATCATCAAGAGTTCCCCTACGACCTAATTTTTGCAGATTTTGCCGATCAAATGAACGGGCACGATAATCGTCGTGGTCACGATCATCATCATCATGATCCAGCAGACCCTCTGGATAATGTGCTACTTTTCAGTAGCCAAGGAAGTAAAGATCCATTTTTGGAGCTCTATGATTGGGCAGAGAACAATAACAACCAGCTGATTGGGAATTATTCTTCAAATGCCAATAACTTGTTCAAAGAAGCCAATGGAGGC

mRNA sequence

ATGTTTTGCAGTTTGTTTGAGATGGAGAATTATCAAGCTGGTGATTTGACCGATGTGATTCGAGCGACCTCCGCCGCCGGGGGAGGCGGCGGTTACGGTGGCGCTGTGTCCTCGGGTTTTTCTCTCGCCGGCGACCGTCTCTGGCCGGATCTTCCCGCCGACGATTCCGTGAATTTCGGAGATCCGTTATTGTTTCCGACCACTACCCAAGATCCATTTCTTCACCACTATACTTCCACCGTCAATTTTGCTTCTGACGGTGGTGGGAGTTTGGCTGCGGTGGCGGAACGGCCGTGTAATATATTTTCACGTGTGTTGCAGATCTCTCCGAGCTCGAAAGTGGGTGGTGGTGTAATTAATACTACGAGTGAGACGGTGGCGGCTGCGGCGGGGAATTCGGCTGAAGCATCGGCTTGCGGCGGTGGCGCCGCCTCCTCCGCCGCCGTAGTTTCGAACCCACTGGAAAACTCCGGGCTGCAGATCTCTCCTCCACGAAATCCGGCCATTAAAAGAAGGAAGAGCCAGGTGAAGAAGGTGGTGTGCATTCCGGCGCCGGCGCCGGCGAACAGCCGGTCAAGCAGCGGCGAAGTGGTTCCTTCTGATCTATGGGCATGGAGAAAGTACGGACAAAAACCCATCAAAGGATCCCCATATCCAAGGGGTTATTATCGGTGCAGCAGCTCCAAGGGCTGCTCTGCCCGAAAACAGGTGGAGCGCAGCCGCACCAACCCCAACATGCTTGTCATCACCTACACCTCGGAGCACAACCACCCATGGCCAACTCAACGCAACGCTCTCGCTGGCTCCACTCGAACCCACTCCTCAAGAAACCCCGCCCCTGCCCATAAAACAAGCCCGAAACACGAGCACAACAACGACAATCTAACCTCCCCATTAACGGGGGCCGTAAAGGGGGAGGAGGTGGAAGAAGACCAGACAACAGAGAAAGCAGCAACAACAGCACCAACAACAATAGATAATGATCATCAAGATCGGGAGGGAGATCATCATCATCAAGAGTTCCCCTACGACCTAATTTTTGCAGATTTTGCCGATCAAATGAACGGGCACGATAATCGTCGTGGTCACGATCATCATCATCATGATCCAGCAGACCCTCTGGATAATGTGCTACTTTTCAGTAGCCAAGGAAGTAAAGATCCATTTTTGGAGCTCTATGATTGGGCAGAGAACAATAACAACCAGCTGATTGGGAATTATTCTTCAAATGCCAATAACTTGTTCAAAGAAGCCAATGGAGGC

Coding sequence (CDS)

ATGTTTTGCAGTTTGTTTGAGATGGAGAATTATCAAGCTGGTGATTTGACCGATGTGATTCGAGCGACCTCCGCCGCCGGGGGAGGCGGCGGTTACGGTGGCGCTGTGTCCTCGGGTTTTTCTCTCGCCGGCGACCGTCTCTGGCCGGATCTTCCCGCCGACGATTCCGTGAATTTCGGAGATCCGTTATTGTTTCCGACCACTACCCAAGATCCATTTCTTCACCACTATACTTCCACCGTCAATTTTGCTTCTGACGGTGGTGGGAGTTTGGCTGCGGTGGCGGAACGGCCGTGTAATATATTTTCACGTGTGTTGCAGATCTCTCCGAGCTCGAAAGTGGGTGGTGGTGTAATTAATACTACGAGTGAGACGGTGGCGGCTGCGGCGGGGAATTCGGCTGAAGCATCGGCTTGCGGCGGTGGCGCCGCCTCCTCCGCCGCCGTAGTTTCGAACCCACTGGAAAACTCCGGGCTGCAGATCTCTCCTCCACGAAATCCGGCCATTAAAAGAAGGAAGAGCCAGGTGAAGAAGGTGGTGTGCATTCCGGCGCCGGCGCCGGCGAACAGCCGGTCAAGCAGCGGCGAAGTGGTTCCTTCTGATCTATGGGCATGGAGAAAGTACGGACAAAAACCCATCAAAGGATCCCCATATCCAAGGGGTTATTATCGGTGCAGCAGCTCCAAGGGCTGCTCTGCCCGAAAACAGGTGGAGCGCAGCCGCACCAACCCCAACATGCTTGTCATCACCTACACCTCGGAGCACAACCACCCATGGCCAACTCAACGCAACGCTCTCGCTGGCTCCACTCGAACCCACTCCTCAAGAAACCCCGCCCCTGCCCATAAAACAAGCCCGAAACACGAGCACAACAACGACAATCTAACCTCCCCATTAACGGGGGCCGTAAAGGGGGAGGAGGTGGAAGAAGACCAGACAACAGAGAAAGCAGCAACAACAGCACCAACAACAATAGATAATGATCATCAAGATCGGGAGGGAGATCATCATCATCAAGAGTTCCCCTACGACCTAATTTTTGCAGATTTTGCCGATCAAATGAACGGGCACGATAATCGTCGTGGTCACGATCATCATCATCATGATCCAGCAGACCCTCTGGATAATGTGCTACTTTTCAGTAGCCAAGGAAGTAAAGATCCATTTTTGGAGCTCTATGATTGGGCAGAGAACAATAACAACCAGCTGATTGGGAATTATTCTTCAAATGCCAATAACTTGTTCAAAGAAGCCAATGGAGGC

Protein sequence

MFCSLFEMENYQAGDLTDVIRATSAAGGGGGYGGAVSSGFSLAGDRLWPDLPADDSVNFGDPLLFPTTTQDPFLHHYTSTVNFASDGGGSLAAVAERPCNIFSRVLQISPSSKVGGGVINTTSETVAAAAGNSAEASACGGGAASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSRNPAPAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDHHHQEFPYDLIFADFADQMNGHDNRRGHDHHHHDPADPLDNVLLFSSQGSKDPFLELYDWAENNNNQLIGNYSSNANNLFKEANGG
Homology
BLAST of MS013392 vs. NCBI nr
Match: XP_022142684.1 (probable WRKY transcription factor 35, partial [Momordica charantia])

HSP 1 Score: 549.3 bits (1414), Expect = 2.9e-152
Identity = 275/285 (96.49%), Postives = 277/285 (97.19%), Query Frame = 0

Query: 137 SACGGGAASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGE 196
           SACGGG ASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGE
Sbjct: 1   SACGGGTASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGE 60

Query: 197 VVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHN 256
           VVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHN
Sbjct: 61  VVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHN 120

Query: 257 HPWPTQRNALAGSTRTHSSRNPAPAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEK 316
           HPWPTQRNALAGSTR+HSSRNPA AHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEK
Sbjct: 121 HPWPTQRNALAGSTRSHSSRNPALAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEK 180

Query: 317 AATTAPTTIDNDHQDREGDHHHQEFPYDLIFADFADQMNGHDNRRGHDHHHHDPADPLDN 376
           AATTAPTTIDNDHQDREGD HHQEFPYDLIFADFADQMNGHDNR  HDHHHHDPAD L+N
Sbjct: 181 AATTAPTTIDNDHQDREGDRHHQEFPYDLIFADFADQMNGHDNRCRHDHHHHDPADLLNN 240

Query: 377 VLLFSSQGSKDPFLELYDWAENNNNQLIGNYSSNANNLFKEANGG 422
           VLLF SQGSKDPFLELYDWAENNNNQ IGNYSSNANNLFKEANGG
Sbjct: 241 VLLFGSQGSKDPFLELYDWAENNNNQQIGNYSSNANNLFKEANGG 285

BLAST of MS013392 vs. NCBI nr
Match: XP_038895360.1 (probable WRKY transcription factor 35 [Benincasa hispida])

HSP 1 Score: 469.2 bits (1206), Expect = 3.8e-128
Identity = 291/445 (65.39%), Postives = 325/445 (73.03%), Query Frame = 0

Query: 1   MFCSLFEM-ENY-QAGDLTDVIRATSAAGGGGGYGGAVSSGFSL---AGDRL-WPDLPAD 60
           MFCSLFEM +NY QAGDLTDVIR TSAA  GG     +SS FSL   +GDRL WP  PAD
Sbjct: 1   MFCSLFEMDDNYQQAGDLTDVIRPTSAAVPGG-----LSSEFSLDPFSGDRLRWPHHPAD 60

Query: 61  D-SVNFGDPLLFPTTT-QDPFLHHYTSTVNFASDGGGSLAAVAERPCNIFSRVLQISPSS 120
           D S+NFGDP LFPT++ +DPFLH + S  NF SDGG    A A++P N+ S +LQISPSS
Sbjct: 61  DSSMNFGDPSLFPTSSVRDPFLHPHFSAFNFTSDGGAEGLAAADQPSNLLSHMLQISPSS 120

Query: 121 KVGGGVINT-TSETVAAAAGNS-AEASACGGGAASSAAVVSNPL---ENSGLQISPPRNP 180
             G GVI+T   E++A    NS + AS CGGG        SNPL   ENSG+QIS PR+ 
Sbjct: 121 --GDGVISTPPCESLATTVRNSPSSASTCGGGGMVPTG-GSNPLCLMENSGIQISSPRSS 180

Query: 181 AIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS 240
           A KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS
Sbjct: 181 ATKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS 240

Query: 241 SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSRNP--APAHKTS 300
           SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTR+H SR P  A A KTS
Sbjct: 241 SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTPATATARKTS 300

Query: 301 PKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDHHHQEFPYDL 360
           PKHE  NDN+    T AVK EE++E+QTT KA T       NDHQD   +  HQEFPYDL
Sbjct: 301 PKHEPTNDNVLP--TAAVKEEEIDENQTTNKATTI------NDHQD---EVDHQEFPYDL 360

Query: 361 IFADFADQMNGHDNRRGHDHHHHDPADPLDN-VLLFSSQG--------SKDPFLELYDWA 420
           IF +F++Q+N HD+    DHHH   ADPL+N +L+FSS G        SKDPFLELYDWA
Sbjct: 361 IFTEFSEQINNHDHDHNRDHHHLS-ADPLNNSMLMFSSHGFSTGNGESSKDPFLELYDWA 414

Query: 421 ENNNNQLIGNYSSNANNLFKEANGG 422
           EN+N            +LFKEA GG
Sbjct: 421 ENSN-----------GSLFKEAKGG 414

BLAST of MS013392 vs. NCBI nr
Match: XP_011659293.1 (probable WRKY transcription factor 14 [Cucumis sativus] >KGN65962.1 hypothetical protein Csa_023328 [Cucumis sativus])

HSP 1 Score: 452.2 bits (1162), Expect = 4.9e-123
Identity = 283/445 (63.60%), Postives = 320/445 (71.91%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSL---AGDR-LWPDLPA 60
           MFCSLFEM+N    QAGDLTDVIR +SA        G  SS FSL   +GDR LW  LPA
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRLSSAP-----VAGHFSSEFSLDPFSGDRQLWSHLPA 60

Query: 61  DD-SVNFGDPLLFPTTTQDPFLHHYTSTVNFASDGGGSLAAVAERPCNIFSRVLQISPSS 120
           D+ S+NFGDPL FP  T+DPFL  + S  NFASDGGG   A  ++P N+ S +LQISPSS
Sbjct: 61  DNSSMNFGDPLSFP--TRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSS 120

Query: 121 KVGGGVINT-TSETVAAAAGNSAEA-SACGGGAASSAAVVSNPL---ENSGLQISPPRNP 180
             G GVI+T   E++A A GNS  + S  GGG        SN L   ENSG+QIS PRN 
Sbjct: 121 --GDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNS 180

Query: 181 AIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS 240
           A KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS
Sbjct: 181 ANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS 240

Query: 241 SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSR--NPAPAHKTS 300
           SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTR+H SR      A+KTS
Sbjct: 241 SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTS 300

Query: 301 PKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDHHHQEFPYDL 360
           PKHE  ND L    T  +K EE++EDQ T+KA TT      ND+Q+ EGD  HQ+FPYDL
Sbjct: 301 PKHERINDILP---TTTIKEEEMDEDQMTDKATTT------NDNQE-EGD--HQDFPYDL 360

Query: 361 IFADFADQMNGHDNRRGHDHHHHDPADPL-DNVLLFSS--------QGSKDPFLELYDWA 420
           IF +FADQMN  ++   HDH+HH  ADPL +N+L+FS+        +GSKDPFLELYDWA
Sbjct: 361 IFTEFADQMNDLEHDNNHDHNHHLSADPLNNNMLMFSNHGFSAGNGEGSKDPFLELYDWA 413

Query: 421 ENNNNQLIGNYSSNANNLFKEANGG 422
           EN           ++ +LFKEA GG
Sbjct: 421 EN-----------SSGSLFKEAKGG 413

BLAST of MS013392 vs. NCBI nr
Match: XP_008465657.1 (PREDICTED: probable WRKY transcription factor 14 isoform X1 [Cucumis melo])

HSP 1 Score: 448.7 bits (1153), Expect = 5.4e-122
Identity = 282/445 (63.37%), Postives = 319/445 (71.69%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSL---AGD-RLWPDLPA 60
           MFCSLFEM+N    QAGDLTDVIR TSA        G  SS FSL   +G+ RLW  LPA
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRPTSAP-----VAGHFSSEFSLDSFSGEGRLWSHLPA 60

Query: 61  DD-SVNFGDPLLFPTTTQDPFLHHYTSTVNFASDGGGSLAAVAERPCNIFSRVLQISPSS 120
           D+ S+NFGDPL F   T+DPFL  + S  NFASDGGG     A++P N+ S +LQISPSS
Sbjct: 61  DNSSMNFGDPLSF--QTRDPFLLPHFSPFNFASDGGGE-GLAADQPSNLLSHMLQISPSS 120

Query: 121 KVGGGVINT-TSETVAAAAGNS-AEASACGGGAASSAAVVSNPL---ENSGLQISPPRNP 180
             G GVI+T   E++A+A GNS +  S  GGG        SNPL   ENSG+QIS PRN 
Sbjct: 121 --GDGVISTPPCESLASAVGNSPSSTSVRGGGGVVVPTGGSNPLCLMENSGIQISSPRNS 180

Query: 181 AIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS 240
           A KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS
Sbjct: 181 ANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS 240

Query: 241 SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSR--NPAPAHKTS 300
           SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTR+H SR       +KTS
Sbjct: 241 SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTTTATNKTS 300

Query: 301 PKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDHHHQEFPYDL 360
           PK E  ND L    T  +K EE++EDQTT+KAATT      ND+Q+ EGD  HQ+FP+DL
Sbjct: 301 PKQERINDILP---TTMIKEEEIDEDQTTDKAATTT-----NDNQE-EGD--HQDFPFDL 360

Query: 361 IFADFADQMNGHDNRRGHDHHHHDPADPL-DNVLLFSS--------QGSKDPFLELYDWA 420
           IF +FADQMN  ++   HDH HH  ADPL +N+L+F S        +GSKDPFLELYDWA
Sbjct: 361 IFTEFADQMNDLEHENDHDHSHHLSADPLNNNMLMFGSHGFSTGNGEGSKDPFLELYDWA 413

Query: 421 ENNNNQLIGNYSSNANNLFKEANGG 422
           EN           ++ +LFKEA GG
Sbjct: 421 EN-----------SSGSLFKEAKGG 413

BLAST of MS013392 vs. NCBI nr
Match: XP_022994821.1 (probable WRKY transcription factor 35 [Cucurbita maxima])

HSP 1 Score: 448.7 bits (1153), Expect = 5.4e-122
Identity = 271/421 (64.37%), Postives = 297/421 (70.55%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSLAGDRLWPDLPADD-S 60
           MFC+LFEM+N    QAGDLTDVIRA+SAA        A SS FSL    L P LPADD S
Sbjct: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAAN-----SAAQSSEFSLGDRHLLPHLPADDSS 60

Query: 61  VNFGDPLLFPT-TTQDPFLHHYTSTVNFASDGGGSLAAVAERPCNIFSRVLQISPSSKVG 120
           +NFGDPLL PT +TQDPFLH + S+ NF S GGG     AERP  +F R+LQISP+SKVG
Sbjct: 61  MNFGDPLLLPTSSTQDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVG 120

Query: 121 GGVINTTSETVAAAAGNSAEASACGGGAASSAAVVSNPL---ENSGLQISPPRNPAIKRR 180
            GVI T      A A      S             SNPL   +NS LQISPPRNPAIKRR
Sbjct: 121 DGVITTPPCDSPAVAATRNSPS-------------SNPLCLRDNSDLQISPPRNPAIKRR 180

Query: 181 KSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCS 240
           K+QVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCS
Sbjct: 181 KNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCS 240

Query: 241 ARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSRNPAPAHK-TSPKHEHN 300
           ARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTR+H SR      + TSPKHEHN
Sbjct: 241 ARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHEHN 300

Query: 301 NDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDHHHQEFPYDLIFADFA 360
           N+NL    T  +K E++ EDQTTEKAATT  T   N HQ  EGD  HQEF YDLIF +F+
Sbjct: 301 NENLA---TMKIKEEDMNEDQTTEKAATTITT---NQHQ-VEGD--HQEFLYDLIFDEFS 360

Query: 361 DQMNGHDNRRGHDHHHHDPADPLDNVLLFSSQG---------SKDPFLELYDWAENNNNQ 404
           DQMN HD+R   D  +       DN+L FS+ G         SKDPFLELYDW E +N  
Sbjct: 361 DQMNEHDHRHATDTMN-------DNLLRFSNHGFSKGNDEERSKDPFLELYDWTEKSNGS 387

BLAST of MS013392 vs. ExPASy Swiss-Prot
Match: Q9SA80 (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 218.4 bits (555), Expect = 1.6e-55
Identity = 175/396 (44.19%), Postives = 214/396 (54.04%), Query Frame = 0

Query: 5   LFEMENYQAGDLTDVIRATSAAGGGGGYGGAVSSGFSLAGDRLWP-------DLPADDSV 64
           L +MEN+Q GDLTDV+R         G GG V S        +WP         P+D ++
Sbjct: 7   LLDMENFQ-GDLTDVVR---------GIGGHVLSP-ETPPSNIWPLPLSHPTPSPSDLNI 66

Query: 65  N-FGDPLLFPTTTQDPFLHH--------YTSTV-----NFASDGGGSLAAVAER-----P 124
           N FGDP +   +  DP L          Y STV     N  ++ G  +  V E       
Sbjct: 67  NPFGDPFV---SMDDPLLQELNSITNSGYFSTVGDNNNNIHNNNGFLVPKVFEEDHIKSQ 126

Query: 125 CNIFSRVLQISPSSKV-GGGVINT---TSETVAAAAGNSAEASA-CGGGAASSAAVVSNP 184
           C+IF R+ +IS S+ +      N+   ++  VAAAA  S          +  +  +V   
Sbjct: 127 CSIFPRI-RISHSNIIHDSSPCNSPAMSAHVVAAAAAASPRGIINVDTNSPRNCLLVDGT 186

Query: 185 LENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPI 244
             +S +QIS PRN  +KRRKSQ KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQKPI
Sbjct: 187 TFSSQIQISSPRNLGLKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQKPI 246

Query: 245 KGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTH 304
           KGSP+PRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWP QRNALAGSTR+ 
Sbjct: 247 KGSPFPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTRSS 306

Query: 305 SSRNPAPAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTE-KAATTAPTTIDNDH-QD 364
           +S +             +N N + P T  V    +    T    ++TT P T+ +   +D
Sbjct: 307 TSSS-------------SNPNPSKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKD 366

Query: 365 REGDHHHQEFPYDLIFADFADQMNGHDNRRGHDHHH 368
             GD    E        D  D          HDH H
Sbjct: 367 ERGDDMELE-----NVDDDDDNQIAPYRPELHDHQH 368

BLAST of MS013392 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.3e-54
Identity = 182/405 (44.94%), Postives = 226/405 (55.80%), Query Frame = 0

Query: 8   MENYQAGDLTDVIRATSAA----GGGGGYGGAVSSGFSLAGDRLWPDLP-ADDSVN---- 67
           M+N+Q GDLTDV+R   +       G   G + SS        L  + P A  S +    
Sbjct: 1   MDNFQ-GDLTDVVRGIGSGHVSPSPGPPEGPSPSSMSPPPTSDLHVEFPSAATSASCLAN 60

Query: 68  -FGDPLLFPTTTQDPFLHHYTSTVNFASDGGGSLAAV----------AERPCNIFSRVLQ 127
            FGDP +   + +DP +H   S ++ A D   + +             +  C++F R+ +
Sbjct: 61  PFGDPFV---SMKDPLIHLPASYISGAGDNKSNKSFAIFPKIFEDDHIKSQCSVFPRI-K 120

Query: 128 ISPSSKV-GGGVINTTSETVAAAAGNSAEASACGGGAASS---AAVVSNPLENSG---LQ 187
           IS S+ +      N+ + TV++AA  ++          +S     +V N    S    +Q
Sbjct: 121 ISQSNNIHDASTCNSPAITVSSAAVAASPWGMINVNTTNSPRNCLLVDNNNNTSSCSQVQ 180

Query: 188 I-SPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP 247
           I S PRN  IKRRKSQ KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQKPIKGSPYP
Sbjct: 181 ISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQKPIKGSPYP 240

Query: 248 RGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSRNPA 307
           RGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTR+ SS +  
Sbjct: 241 RGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLN 300

Query: 308 PAHK-------TSP----------KHEHNNDNLTSPLT------GAVKGEEVEEDQTTEK 357
           P+ K       TSP          K E NN NL S  T       A+K E VEE Q  EK
Sbjct: 301 PSSKSSTAAATTSPSSRVFQNNSSKDEPNNSNLPSSSTHPPFDAAAIKEENVEERQ--EK 360

BLAST of MS013392 vs. ExPASy Swiss-Prot
Match: Q9LP56 (Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.7e-31
Identity = 70/120 (58.33%), Postives = 80/120 (66.67%), Query Frame = 0

Query: 170 KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 229
           + R+S  K+VV +P      SR       PSD WAWRKYGQKPIKGSPYPRGYYRCSS+K
Sbjct: 44  RSRRSVEKRVVNVPMKEMEGSRHKGDTTPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSTK 103

Query: 230 GCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSRNPAPAHKTSPKHE 289
           GC ARKQVERSR +P M++ITYTSEHNHPWP     L  STR      P P  +  P+ E
Sbjct: 104 GCPARKQVERSRDDPTMILITYTSEHNHPWP-----LTSSTRNGPKPKPEPKPEPEPEVE 158

BLAST of MS013392 vs. ExPASy Swiss-Prot
Match: Q9FLX8 (Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 3.5e-31
Identity = 79/182 (43.41%), Postives = 107/182 (58.79%), Query Frame = 0

Query: 160 QISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP 219
           Q  PP     ++RK+Q K+ +C            + E + SDLWAWRKYGQKPIKGSPYP
Sbjct: 137 QSQPPLRS--RKRKNQQKRTIC----------HVTQENLSSDLWAWRKYGQKPIKGSPYP 196

Query: 220 RGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSR-NP 279
           R YYRCSSSKGC ARKQVERS  +PN+ ++TYT EH HP PT RN+LAGSTR  S   NP
Sbjct: 197 RNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEHTHPRPTHRNSLAGSTRNKSQPVNP 256

Query: 280 APAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDHHH 339
            P   TSP  +   + +    T  +KG +  ++   ++        ++ + ++ E +   
Sbjct: 257 VPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQETNGDEDMVGQEVNMEEEEEEEEVEEDD 306

Query: 340 QE 341
           +E
Sbjct: 317 EE 306

BLAST of MS013392 vs. ExPASy Swiss-Prot
Match: Q9FL92 (Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.0e-30
Identity = 80/152 (52.63%), Postives = 97/152 (63.82%), Query Frame = 0

Query: 170  KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 229
            +R+ ++ K+VVC+       SRS       SDLW WRKYGQKPIK SPYPR YYRC+SSK
Sbjct: 1160 RRKNNKEKRVVCV---VDRGSRS-------SDLWVWRKYGQKPIKSSPYPRSYYRCASSK 1219

Query: 230  GCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSR---------NPAP 289
            GC ARKQVERSRT+PN+ VITY SEHNHP+PT RN LAGSTR+ SS+         +   
Sbjct: 1220 GCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASSTVS 1279

Query: 290  AHKTSPKHEHNNDNLTSPLTGA--VKGEEVEE 311
              K  P   H   +  SP   A  VK E++E+
Sbjct: 1280 QDKEGPDKSHLPSSPASPPYAAMVVKEEDMEQ 1301

BLAST of MS013392 vs. ExPASy TrEMBL
Match: A0A6J1CM64 (probable WRKY transcription factor 35 OS=Momordica charantia OX=3673 GN=LOC111012741 PE=4 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.4e-152
Identity = 275/285 (96.49%), Postives = 277/285 (97.19%), Query Frame = 0

Query: 137 SACGGGAASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGE 196
           SACGGG ASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGE
Sbjct: 1   SACGGGTASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGE 60

Query: 197 VVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHN 256
           VVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHN
Sbjct: 61  VVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHN 120

Query: 257 HPWPTQRNALAGSTRTHSSRNPAPAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEK 316
           HPWPTQRNALAGSTR+HSSRNPA AHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEK
Sbjct: 121 HPWPTQRNALAGSTRSHSSRNPALAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEK 180

Query: 317 AATTAPTTIDNDHQDREGDHHHQEFPYDLIFADFADQMNGHDNRRGHDHHHHDPADPLDN 376
           AATTAPTTIDNDHQDREGD HHQEFPYDLIFADFADQMNGHDNR  HDHHHHDPAD L+N
Sbjct: 181 AATTAPTTIDNDHQDREGDRHHQEFPYDLIFADFADQMNGHDNRCRHDHHHHDPADLLNN 240

Query: 377 VLLFSSQGSKDPFLELYDWAENNNNQLIGNYSSNANNLFKEANGG 422
           VLLF SQGSKDPFLELYDWAENNNNQ IGNYSSNANNLFKEANGG
Sbjct: 241 VLLFGSQGSKDPFLELYDWAENNNNQQIGNYSSNANNLFKEANGG 285

BLAST of MS013392 vs. ExPASy TrEMBL
Match: A0A0A0LYP6 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G547510 PE=4 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 2.4e-123
Identity = 283/445 (63.60%), Postives = 320/445 (71.91%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSL---AGDR-LWPDLPA 60
           MFCSLFEM+N    QAGDLTDVIR +SA        G  SS FSL   +GDR LW  LPA
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRLSSAP-----VAGHFSSEFSLDPFSGDRQLWSHLPA 60

Query: 61  DD-SVNFGDPLLFPTTTQDPFLHHYTSTVNFASDGGGSLAAVAERPCNIFSRVLQISPSS 120
           D+ S+NFGDPL FP  T+DPFL  + S  NFASDGGG   A  ++P N+ S +LQISPSS
Sbjct: 61  DNSSMNFGDPLSFP--TRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSS 120

Query: 121 KVGGGVINT-TSETVAAAAGNSAEA-SACGGGAASSAAVVSNPL---ENSGLQISPPRNP 180
             G GVI+T   E++A A GNS  + S  GGG        SN L   ENSG+QIS PRN 
Sbjct: 121 --GDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNS 180

Query: 181 AIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS 240
           A KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS
Sbjct: 181 ANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS 240

Query: 241 SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSR--NPAPAHKTS 300
           SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTR+H SR      A+KTS
Sbjct: 241 SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTS 300

Query: 301 PKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDHHHQEFPYDL 360
           PKHE  ND L    T  +K EE++EDQ T+KA TT      ND+Q+ EGD  HQ+FPYDL
Sbjct: 301 PKHERINDILP---TTTIKEEEMDEDQMTDKATTT------NDNQE-EGD--HQDFPYDL 360

Query: 361 IFADFADQMNGHDNRRGHDHHHHDPADPL-DNVLLFSS--------QGSKDPFLELYDWA 420
           IF +FADQMN  ++   HDH+HH  ADPL +N+L+FS+        +GSKDPFLELYDWA
Sbjct: 361 IFTEFADQMNDLEHDNNHDHNHHLSADPLNNNMLMFSNHGFSAGNGEGSKDPFLELYDWA 413

Query: 421 ENNNNQLIGNYSSNANNLFKEANGG 422
           EN           ++ +LFKEA GG
Sbjct: 421 EN-----------SSGSLFKEAKGG 413

BLAST of MS013392 vs. ExPASy TrEMBL
Match: A0A6J1K0D1 (probable WRKY transcription factor 35 OS=Cucurbita maxima OX=3661 GN=LOC111490435 PE=4 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 2.6e-122
Identity = 271/421 (64.37%), Postives = 297/421 (70.55%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSLAGDRLWPDLPADD-S 60
           MFC+LFEM+N    QAGDLTDVIRA+SAA        A SS FSL    L P LPADD S
Sbjct: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAAN-----SAAQSSEFSLGDRHLLPHLPADDSS 60

Query: 61  VNFGDPLLFPT-TTQDPFLHHYTSTVNFASDGGGSLAAVAERPCNIFSRVLQISPSSKVG 120
           +NFGDPLL PT +TQDPFLH + S+ NF S GGG     AERP  +F R+LQISP+SKVG
Sbjct: 61  MNFGDPLLLPTSSTQDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVG 120

Query: 121 GGVINTTSETVAAAAGNSAEASACGGGAASSAAVVSNPL---ENSGLQISPPRNPAIKRR 180
            GVI T      A A      S             SNPL   +NS LQISPPRNPAIKRR
Sbjct: 121 DGVITTPPCDSPAVAATRNSPS-------------SNPLCLRDNSDLQISPPRNPAIKRR 180

Query: 181 KSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCS 240
           K+QVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCS
Sbjct: 181 KNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCS 240

Query: 241 ARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSRNPAPAHK-TSPKHEHN 300
           ARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTR+H SR      + TSPKHEHN
Sbjct: 241 ARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHEHN 300

Query: 301 NDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDHHHQEFPYDLIFADFA 360
           N+NL    T  +K E++ EDQTTEKAATT  T   N HQ  EGD  HQEF YDLIF +F+
Sbjct: 301 NENLA---TMKIKEEDMNEDQTTEKAATTITT---NQHQ-VEGD--HQEFLYDLIFDEFS 360

Query: 361 DQMNGHDNRRGHDHHHHDPADPLDNVLLFSSQG---------SKDPFLELYDWAENNNNQ 404
           DQMN HD+R   D  +       DN+L FS+ G         SKDPFLELYDW E +N  
Sbjct: 361 DQMNEHDHRHATDTMN-------DNLLRFSNHGFSKGNDEERSKDPFLELYDWTEKSNGS 387

BLAST of MS013392 vs. ExPASy TrEMBL
Match: A0A1S3CQV3 (probable WRKY transcription factor 14 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503297 PE=4 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 2.6e-122
Identity = 282/445 (63.37%), Postives = 319/445 (71.69%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSL---AGD-RLWPDLPA 60
           MFCSLFEM+N    QAGDLTDVIR TSA        G  SS FSL   +G+ RLW  LPA
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRPTSAP-----VAGHFSSEFSLDSFSGEGRLWSHLPA 60

Query: 61  DD-SVNFGDPLLFPTTTQDPFLHHYTSTVNFASDGGGSLAAVAERPCNIFSRVLQISPSS 120
           D+ S+NFGDPL F   T+DPFL  + S  NFASDGGG     A++P N+ S +LQISPSS
Sbjct: 61  DNSSMNFGDPLSF--QTRDPFLLPHFSPFNFASDGGGE-GLAADQPSNLLSHMLQISPSS 120

Query: 121 KVGGGVINT-TSETVAAAAGNS-AEASACGGGAASSAAVVSNPL---ENSGLQISPPRNP 180
             G GVI+T   E++A+A GNS +  S  GGG        SNPL   ENSG+QIS PRN 
Sbjct: 121 --GDGVISTPPCESLASAVGNSPSSTSVRGGGGVVVPTGGSNPLCLMENSGIQISSPRNS 180

Query: 181 AIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS 240
           A KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS
Sbjct: 181 ANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS 240

Query: 241 SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSR--NPAPAHKTS 300
           SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTR+H SR       +KTS
Sbjct: 241 SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTTTATNKTS 300

Query: 301 PKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDHHHQEFPYDL 360
           PK E  ND L    T  +K EE++EDQTT+KAATT      ND+Q+ EGD  HQ+FP+DL
Sbjct: 301 PKQERINDILP---TTMIKEEEIDEDQTTDKAATTT-----NDNQE-EGD--HQDFPFDL 360

Query: 361 IFADFADQMNGHDNRRGHDHHHHDPADPL-DNVLLFSS--------QGSKDPFLELYDWA 420
           IF +FADQMN  ++   HDH HH  ADPL +N+L+F S        +GSKDPFLELYDWA
Sbjct: 361 IFTEFADQMNDLEHENDHDHSHHLSADPLNNNMLMFGSHGFSTGNGEGSKDPFLELYDWA 413

Query: 421 ENNNNQLIGNYSSNANNLFKEANGG 422
           EN           ++ +LFKEA GG
Sbjct: 421 EN-----------SSGSLFKEAKGG 413

BLAST of MS013392 vs. ExPASy TrEMBL
Match: A0A6J1GSN9 (probable WRKY transcription factor 35 OS=Cucurbita moschata OX=3662 GN=LOC111457137 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 4.4e-122
Identity = 271/423 (64.07%), Postives = 297/423 (70.21%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSLAGDRLWPDLPADD-S 60
           MFC+LFEM+N    QAGDLTDVIRA+SAA        A SS FSL    L P LPADD S
Sbjct: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAA-----TSAAHSSEFSLGDRHLLPHLPADDSS 60

Query: 61  VNFGDPLLFPT-TTQDPFLHHYTSTVNFASDGGGSLAAVAERPCNIFSRVLQISPSSKVG 120
           +NFGDPLL PT +T+DPFLH + S+ NF S GGG     AERP  +F R+LQISP+SKVG
Sbjct: 61  MNFGDPLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVG 120

Query: 121 GGVINT--TSETVAAAAGNSAEASACGGGAASSAAVVSNPL---ENSGLQISPPRNPAIK 180
            GVI T        A  GNS                 SNPL   +NS LQISPPRNPA+K
Sbjct: 121 DGVITTPPCDSPAVAVTGNSP---------------TSNPLCLRDNSDLQISPPRNPALK 180

Query: 181 RRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 240
           RRK+QVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG
Sbjct: 181 RRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 240

Query: 241 CSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSRNPAPAHK-TSPKHE 300
           CSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTR+H SR      + TSPKHE
Sbjct: 241 CSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHE 300

Query: 301 HNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDHHHQEFPYDLIFAD 360
           HNN+NL    T  +K E++ EDQTTEKA TT  T   N HQ  EGD  HQEF YDLIF +
Sbjct: 301 HNNENLA---TVTIKEEDMNEDQTTEKATTTITT---NQHQ-VEGD--HQEFLYDLIFDE 360

Query: 361 FADQMNGHDNRRGHDHHHHDPADPLDNVLLFSSQG---------SKDPFLELYDWAENNN 404
           FADQMN HD      HH  D  +  DN+L FS+ G         SKDPFLELYDW E +N
Sbjct: 361 FADQMNEHDR-----HHATDTMN--DNLLRFSNHGFSKGNDEERSKDPFLELYDWTEKSN 387

BLAST of MS013392 vs. TAIR 10
Match: AT1G30650.1 (WRKY DNA-binding protein 14 )

HSP 1 Score: 218.4 bits (555), Expect = 1.1e-56
Identity = 175/396 (44.19%), Postives = 214/396 (54.04%), Query Frame = 0

Query: 5   LFEMENYQAGDLTDVIRATSAAGGGGGYGGAVSSGFSLAGDRLWP-------DLPADDSV 64
           L +MEN+Q GDLTDV+R         G GG V S        +WP         P+D ++
Sbjct: 7   LLDMENFQ-GDLTDVVR---------GIGGHVLSP-ETPPSNIWPLPLSHPTPSPSDLNI 66

Query: 65  N-FGDPLLFPTTTQDPFLHH--------YTSTV-----NFASDGGGSLAAVAER-----P 124
           N FGDP +   +  DP L          Y STV     N  ++ G  +  V E       
Sbjct: 67  NPFGDPFV---SMDDPLLQELNSITNSGYFSTVGDNNNNIHNNNGFLVPKVFEEDHIKSQ 126

Query: 125 CNIFSRVLQISPSSKV-GGGVINT---TSETVAAAAGNSAEASA-CGGGAASSAAVVSNP 184
           C+IF R+ +IS S+ +      N+   ++  VAAAA  S          +  +  +V   
Sbjct: 127 CSIFPRI-RISHSNIIHDSSPCNSPAMSAHVVAAAAAASPRGIINVDTNSPRNCLLVDGT 186

Query: 185 LENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPI 244
             +S +QIS PRN  +KRRKSQ KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQKPI
Sbjct: 187 TFSSQIQISSPRNLGLKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQKPI 246

Query: 245 KGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTH 304
           KGSP+PRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWP QRNALAGSTR+ 
Sbjct: 247 KGSPFPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTRSS 306

Query: 305 SSRNPAPAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTE-KAATTAPTTIDNDH-QD 364
           +S +             +N N + P T  V    +    T    ++TT P T+ +   +D
Sbjct: 307 TSSS-------------SNPNPSKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKD 366

Query: 365 REGDHHHQEFPYDLIFADFADQMNGHDNRRGHDHHH 368
             GD    E        D  D          HDH H
Sbjct: 367 ERGDDMELE-----NVDDDDDNQIAPYRPELHDHQH 368

BLAST of MS013392 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 215.3 bits (547), Expect = 9.3e-56
Identity = 182/405 (44.94%), Postives = 226/405 (55.80%), Query Frame = 0

Query: 8   MENYQAGDLTDVIRATSAA----GGGGGYGGAVSSGFSLAGDRLWPDLP-ADDSVN---- 67
           M+N+Q GDLTDV+R   +       G   G + SS        L  + P A  S +    
Sbjct: 1   MDNFQ-GDLTDVVRGIGSGHVSPSPGPPEGPSPSSMSPPPTSDLHVEFPSAATSASCLAN 60

Query: 68  -FGDPLLFPTTTQDPFLHHYTSTVNFASDGGGSLAAV----------AERPCNIFSRVLQ 127
            FGDP +   + +DP +H   S ++ A D   + +             +  C++F R+ +
Sbjct: 61  PFGDPFV---SMKDPLIHLPASYISGAGDNKSNKSFAIFPKIFEDDHIKSQCSVFPRI-K 120

Query: 128 ISPSSKV-GGGVINTTSETVAAAAGNSAEASACGGGAASS---AAVVSNPLENSG---LQ 187
           IS S+ +      N+ + TV++AA  ++          +S     +V N    S    +Q
Sbjct: 121 ISQSNNIHDASTCNSPAITVSSAAVAASPWGMINVNTTNSPRNCLLVDNNNNTSSCSQVQ 180

Query: 188 I-SPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP 247
           I S PRN  IKRRKSQ KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQKPIKGSPYP
Sbjct: 181 ISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQKPIKGSPYP 240

Query: 248 RGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSRNPA 307
           RGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTR+ SS +  
Sbjct: 241 RGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLN 300

Query: 308 PAHK-------TSP----------KHEHNNDNLTSPLT------GAVKGEEVEEDQTTEK 357
           P+ K       TSP          K E NN NL S  T       A+K E VEE Q  EK
Sbjct: 301 PSSKSSTAAATTSPSSRVFQNNSSKDEPNNSNLPSSSTHPPFDAAAIKEENVEERQ--EK 360

BLAST of MS013392 vs. TAIR 10
Match: AT1G29280.1 (WRKY DNA-binding protein 65 )

HSP 1 Score: 137.9 bits (346), Expect = 1.9e-32
Identity = 70/120 (58.33%), Postives = 80/120 (66.67%), Query Frame = 0

Query: 170 KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 229
           + R+S  K+VV +P      SR       PSD WAWRKYGQKPIKGSPYPRGYYRCSS+K
Sbjct: 44  RSRRSVEKRVVNVPMKEMEGSRHKGDTTPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSTK 103

Query: 230 GCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSRNPAPAHKTSPKHE 289
           GC ARKQVERSR +P M++ITYTSEHNHPWP     L  STR      P P  +  P+ E
Sbjct: 104 GCPARKQVERSRDDPTMILITYTSEHNHPWP-----LTSSTRNGPKPKPEPKPEPEPEVE 158

BLAST of MS013392 vs. TAIR 10
Match: AT5G52830.1 (WRKY DNA-binding protein 27 )

HSP 1 Score: 137.5 bits (345), Expect = 2.5e-32
Identity = 79/182 (43.41%), Postives = 107/182 (58.79%), Query Frame = 0

Query: 160 QISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP 219
           Q  PP     ++RK+Q K+ +C            + E + SDLWAWRKYGQKPIKGSPYP
Sbjct: 137 QSQPPLRS--RKRKNQQKRTIC----------HVTQENLSSDLWAWRKYGQKPIKGSPYP 196

Query: 220 RGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSR-NP 279
           R YYRCSSSKGC ARKQVERS  +PN+ ++TYT EH HP PT RN+LAGSTR  S   NP
Sbjct: 197 RNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEHTHPRPTHRNSLAGSTRNKSQPVNP 256

Query: 280 APAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDHHH 339
            P   TSP  +   + +    T  +KG +  ++   ++        ++ + ++ E +   
Sbjct: 257 VPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQETNGDEDMVGQEVNMEEEEEEEEVEEDD 306

Query: 340 QE 341
           +E
Sbjct: 317 EE 306

BLAST of MS013392 vs. TAIR 10
Match: AT5G45050.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 136.0 bits (341), Expect = 7.2e-32
Identity = 80/152 (52.63%), Postives = 97/152 (63.82%), Query Frame = 0

Query: 170  KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 229
            +R+ ++ K+VVC+       SRS       SDLW WRKYGQKPIK SPYPR YYRC+SSK
Sbjct: 1160 RRKNNKEKRVVCV---VDRGSRS-------SDLWVWRKYGQKPIKSSPYPRSYYRCASSK 1219

Query: 230  GCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRTHSSR---------NPAP 289
            GC ARKQVERSRT+PN+ VITY SEHNHP+PT RN LAGSTR+ SS+         +   
Sbjct: 1220 GCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASSTVS 1279

Query: 290  AHKTSPKHEHNNDNLTSPLTGA--VKGEEVEE 311
              K  P   H   +  SP   A  VK E++E+
Sbjct: 1280 QDKEGPDKSHLPSSPASPPYAAMVVKEEDMEQ 1301

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142684.12.9e-15296.49probable WRKY transcription factor 35, partial [Momordica charantia][more]
XP_038895360.13.8e-12865.39probable WRKY transcription factor 35 [Benincasa hispida][more]
XP_011659293.14.9e-12363.60probable WRKY transcription factor 14 [Cucumis sativus] >KGN65962.1 hypothetical... [more]
XP_008465657.15.4e-12263.37PREDICTED: probable WRKY transcription factor 14 isoform X1 [Cucumis melo][more]
XP_022994821.15.4e-12264.37probable WRKY transcription factor 35 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SA801.6e-5544.19Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ... [more]
O647471.3e-5444.94Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ... [more]
Q9LP562.7e-3158.33Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 ... [more]
Q9FLX83.5e-3143.41Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 ... [more]
Q9FL921.0e-3052.63Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1CM641.4e-15296.49probable WRKY transcription factor 35 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A0A0LYP62.4e-12363.60WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G547510 PE=4 S... [more]
A0A6J1K0D12.6e-12264.37probable WRKY transcription factor 35 OS=Cucurbita maxima OX=3661 GN=LOC11149043... [more]
A0A1S3CQV32.6e-12263.37probable WRKY transcription factor 14 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1GSN94.4e-12264.07probable WRKY transcription factor 35 OS=Cucurbita moschata OX=3662 GN=LOC111457... [more]
Match NameE-valueIdentityDescription
AT1G30650.11.1e-5644.19WRKY DNA-binding protein 14 [more]
AT2G34830.19.3e-5644.94WRKY DNA-binding protein 35 [more]
AT1G29280.11.9e-3258.33WRKY DNA-binding protein 65 [more]
AT5G52830.12.5e-3243.41WRKY DNA-binding protein 27 [more]
AT5G45050.17.2e-3252.63Disease resistance protein (TIR-NBS-LRR class) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 199..259
e-value: 6.0E-37
score: 138.7
IPR003657WRKY domainPFAMPF03106WRKYcoord: 201..258
e-value: 4.3E-25
score: 87.7
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 194..260
score: 32.535049
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 172..253
e-value: 3.8E-30
score: 105.3
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 197..260
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 252..281
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 252..336
NoneNo IPR availablePANTHERPTHR32096:SF18WRKY TRANSCRIPTION FACTOR 14-RELATEDcoord: 96..398
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 96..398

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS013392.1MS013392.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding