MS013380 (gene) Bitter gourd (TR) v1

BLAST of MS013380 vs. NCBI nr
Match: XP_022142682.1 (alpha-glucosidase-like [Momordica charantia])

HSP 1 Score: 1735.3 bits (4493), Expect = 0.0e+00
Identity = 862/899 (95.88%), Postives = 871/899 (96.89%), Query Frame = 0

Query: 8   MAKTATRSLFGFSLFI-VCFFF-SLAEPQPSPVVGYGYRIRSAHVDPAGKSLTAGLALI- 67
           M +TATR+  G S FI +CFFF  LA+P P P  G+GYRIRS  + P GKSLTA LALI 
Sbjct: 27  MQRTATRATSGLSFFILICFFFLCLAQPPPPPPAGHGYRIRSVQLHPTGKSLTADLALIG 86

Query: 68  RSSSVYGSDLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSPPESHV 127
            SSSVYG DL TLTLQAS ETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRS PESHV
Sbjct: 87  TSSSVYGPDLPTLTLQASFETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSLPESHV 146

Query: 128 DSPATASISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ 187
           DSPATA ISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ
Sbjct: 147 DSPATAFISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ 206

Query: 188 LSSSLPKDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVR 247
           LSS LP DRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVR
Sbjct: 207 LSSLLPNDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVR 266

Query: 248 SPSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYT 307
           SPSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYT
Sbjct: 267 SPSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYT 326

Query: 308 ELIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFT 367
           ELIGRPAPVPYWSFG+HQCRYGYKNVSDVENVVARYAK GIPLEVMWTDIDYMDGYKDFT
Sbjct: 327 ELIGRPAPVPYWSFGFHQCRYGYKNVSDVENVVARYAKAGIPLEVMWTDIDYMDGYKDFT 386

Query: 368 FDPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYS 427
           FDPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYS
Sbjct: 387 FDPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYS 446

Query: 428 GEVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLD 487
           GEVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLD
Sbjct: 447 GEVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLD 506

Query: 488 NPPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPF 547
           NPPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPF
Sbjct: 507 NPPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPF 566

Query: 548 VVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEE 607
           VVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEE
Sbjct: 567 VVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEE 626

Query: 608 LCRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAH 667
           LCRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAH
Sbjct: 627 LCRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAH 686

Query: 668 RKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFN 727
           RKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFN
Sbjct: 687 RKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFN 746

Query: 728 YSESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQS 787
           YSESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQS
Sbjct: 747 YSESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQS 806

Query: 788 SDGEVFLDDGEVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTF 847
           SDGEVFLDDG VVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFAL+QKLIIDKVTF
Sbjct: 807 SDGEVFLDDGGVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALTQKLIIDKVTF 866

Query: 848 IGFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVDISALSIPIWEEFKLELT 904
           IGFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNV+ISALSIPIWEEFKLELT
Sbjct: 867 IGFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVEISALSIPIWEEFKLELT 925

BLAST of MS013380 vs. NCBI nr
Match: XP_023001112.1 (alpha-glucosidase [Cucurbita maxima])

HSP 1 Score: 1541.9 bits (3991), Expect = 0.0e+00
Identity = 753/891 (84.51%), Postives = 808/891 (90.68%), Query Frame = 0

Query: 16  LFGFSLF---IVCFFFSLAEPQPSPVVGYGYRIRSAHVDPAGKSLTAGLALIRSSSVYGS 75
           LF FS F   I    F+  E + +  VGYGYR+RS  VDP GKSLTA L LI  SSVYG 
Sbjct: 44  LFLFSCFFRLIASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGP 103

Query: 76  DLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSPPESHVDSPATASI 135
           D+  L+LQAS ETKDRLR+RITDS RERWEIPD IIPRRSNSR RS PE  V SP T+ I
Sbjct: 104 DVERLSLQASFETKDRLRVRITDSNRERWEIPDEIIPRRSNSRIRSLPERDVGSPETSII 163

Query: 136 SDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKD 195
           SDPASDL FSL +T PFGFSV RRSSGDVLFDT+P+ SD  TFLVFKDQYIQLSSSLPKD
Sbjct: 164 SDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKD 223

Query: 196 RSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRSPSRDGKV 255
           RSSLFGIGEQTR+SFK+V +KSKTLTLW+ADIG    DVNLYG+HPFYIDVRSPSRDGKV
Sbjct: 224 RSSLFGIGEQTRKSFKIVPDKSKTLTLWSADIGSVNTDVNLYGAHPFYIDVRSPSRDGKV 283

Query: 256 VAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAP 315
            AGTTHGVLLLNSNGMDIIYSGDRI+YK IGG+IDLYFFAGPSP+SVIDQYTELIGRPAP
Sbjct: 284 AAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAP 343

Query: 316 VPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTFDPINFPA 375
           VPYWSFG+HQCRYGYKNVSD+E VVARYAK GIPLEVMWTDIDYMDGYKDFTFDPINFP 
Sbjct: 344 VPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPV 403

Query: 376 EKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYSGEVWSGPV 435
           EKMK FVD+LHQNGQKYV I+DPGIS NKTYGT+IRG +ADIFIKY+GVPY GEVW GPV
Sbjct: 404 EKMKNFVDSLHQNGQKYVLIVDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPV 463

Query: 436 YFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINN 495
           YFPDF+HPNSEIFWGGEIKLFRDIVPFDGLWIDMNE+SN ITSSTSP SNLDNPPYKI+N
Sbjct: 464 YFPDFLHPNSEIFWGGEIKLFRDIVPFDGLWIDMNELSNFITSSTSPFSNLDNPPYKIDN 523

Query: 496 AAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVVSRSTFV 555
           A V RPINN TVPASSLHFGNLTEYNTHNLYG LES+ATHASLVKVTGKRPFV+SRSTFV
Sbjct: 524 AGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFV 583

Query: 556 GSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEELCRRWIQL 615
           GSGKYTAHWTGDN ATWNDLGYTIPSILNFGLFGIPMVG+DICGF+GDTTEELCRRWIQL
Sbjct: 584 GSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELCRRWIQL 643

Query: 616 GAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHRKGTPIAR 675
           GAFYPFAR HSDK  IR+ELYLWD+VAA+ARKVLALRY LLPY YTLMYEAH+KGTPIAR
Sbjct: 644 GAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPIAR 703

Query: 676 PLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNYSESVSVK 735
           PLFFSFPQDI+TYEI+SQFLLG+GVL+SPVLKEGALSVDAYFP GNWFSLFNYSESV+VK
Sbjct: 704 PLFFSFPQDIRTYEINSQFLLGEGVLVSPVLKEGALSVDAYFPAGNWFSLFNYSESVAVK 763

Query: 736 SGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSSDGEVFLD 795
           SGQQ+TLDAPADHINVHVREGNIL LHG+A TT+AAR TAFKLLVVVSNGQSS GEVFLD
Sbjct: 764 SGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLD 823

Query: 796 DGEVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTFIGFERHEK 855
           DGEVVEMGEEGGNWSLVRF+SEAVGSKLLIKS+V+NGGFALSQK+IIDKVTF+GFER +K
Sbjct: 824 DGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTFVGFERPKK 883

Query: 856 MGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVDISALSIPIWEEFKLELT 904
           MGG GL ISKGANLNGNS I+KTYEYS KFVNV+IS LSIPI EEF +EL+
Sbjct: 884 MGGLGLDISKGANLNGNSGIRKTYEYSAKFVNVEISGLSIPICEEFVMELS 934

BLAST of MS013380 vs. NCBI nr
Match: XP_023520110.1 (alpha-glucosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1533.1 bits (3968), Expect = 0.0e+00
Identity = 758/918 (82.57%), Postives = 812/918 (88.45%), Query Frame = 0

Query: 4   SSITMAKTATRSLFGF----------SLFI-VCFF-------FSLAEPQPSPVVGYGYRI 63
           SS TMA T ++SL  F           LF+  CFF       F+  E + +  VGYGYR+
Sbjct: 18  SSTTMA-TGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRV 77

Query: 64  RSAHVDPAGKSLTAGLALIRSSSVYGSDLHTLTLQASLETKDRLRIRITDSARERWEIPD 123
           RS  VDP GKSLTA L LI  SSVYG D+  L++QAS ETKDRLR+RITDS RERWE+PD
Sbjct: 78  RSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRLRVRITDSNRERWEVPD 137

Query: 124 NIIPRRSNSRFRSPPESHVDSPATASISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDT 183
            IIPRRSNSR RS PE  V SP T+ ISDPASDL FSL +T PFGFSV RRSSGDVLFDT
Sbjct: 138 EIIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDT 197

Query: 184 APDLSDPGTFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIG 243
           +P+ SD  TFLVFKDQYIQLSSSLPKDRSSLFGIGEQTR+SFKLV +KSKTLTLWNADIG
Sbjct: 198 SPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIG 257

Query: 244 RAIVDVNLYGSHPFYIDVRSPSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGV 303
               DVNLYG+HPFYIDVRSPSRDGKV AGTTHGVLLLNSNGMDIIYSGDRI+YK IGG+
Sbjct: 258 SVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGI 317

Query: 304 IDLYFFAGPSPVSVIDQYTELIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGI 363
           IDLYFFAGPSP+SVIDQYTELIGRPAPVPYWSFG+HQCRYGYKNVSD+E VVARYAK GI
Sbjct: 318 IDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGI 377

Query: 364 PLEVMWTDIDYMDGYKDFTFDPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGT 423
           PLEVMWTDIDYMDGYKDFTFDPINFP EKMK FVDNLHQNGQKYV ILDPGIS NKTYGT
Sbjct: 378 PLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGT 437

Query: 424 FIRGTKADIFIKYNGVPYSGEVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWID 483
           +IRG +ADIFIKY+GVPY GEVW GPVYFPDF+HPNSEIFWG EIKLFRDIVPFDGLWID
Sbjct: 438 YIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWID 497

Query: 484 MNEISNAITSSTSPLSNLDNPPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGL 543
           MNEISN ITSSTS  SNLDNPPYKINNA V RPINN TVPASSLHFGNLTEYNTHNLYG 
Sbjct: 498 MNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGF 557

Query: 544 LESRATHASLVKVTGKRPFVVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLF 603
           LES+ATHASLVKVTGKRPFV+SRSTFVGSGKYTAHWTGDN ATWNDLGYTIPSILNFGLF
Sbjct: 558 LESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLF 617

Query: 604 GIPMVGSDICGFAGDTTEELCRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKV 663
           GIPMVG+DICGF+ DTTEELCRRWIQLGAFYPFAR HSDK  IR+ELYLWD+VAA+ARKV
Sbjct: 618 GIPMVGADICGFSRDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKV 677

Query: 664 LALRYRLLPYFYTLMYEAHRKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKE 723
           LALRY LLPY YTLMYEAH+KGTPIARPLFFSFPQDI+TYEI+SQFLLG GVLISPVLKE
Sbjct: 678 LALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKE 737

Query: 724 GALSVDAYFPEGNWFSLFNYSESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTT 783
           GA+SVDAYFP GNWFSLFNYSESV+V SGQQ+TLDAPADHINVHVREGNIL LHG+A TT
Sbjct: 738 GAISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVHVREGNILALHGEATTT 797

Query: 784 RAARGTAFKLLVVVSNGQSSDGEVFLDDGEVVEMGEEGGNWSLVRFHSEAVGSKLLIKSE 843
           +AAR TAFKLLVVVSNGQSS GEVFLDDGEVVEMGEEGGNWSLVRF+SEAVGSKLLIKS+
Sbjct: 798 QAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQ 857

Query: 844 VVNGGFALSQKLIIDKVTFIGFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNV 903
           V+NGGFALSQK+IIDKVT +GFER + MGG GL ISKGANL+GNS I+KTYEYS KFVNV
Sbjct: 858 VINGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGNSGIRKTYEYSAKFVNV 917

BLAST of MS013380 vs. NCBI nr
Match: KAG6583815.1 (hypothetical protein SDJN03_19747, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019441.1 hypothetical protein SDJN02_18402 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1528.8 bits (3957), Expect = 0.0e+00
Identity = 747/898 (83.18%), Postives = 802/898 (89.31%), Query Frame = 0

Query: 14  RSLFGFSLFI-VCFF-------FSLAEPQPSPVVGYGYRIRSAHVDPAGKSLTAGLALIR 73
           R L    LF+  CFF       F+  E + +  VGYGYR+RS  VDP GKSLTA L LI 
Sbjct: 16  RLLSAVVLFLFTCFFRLTASLPFAAGEKRENSAVGYGYRVRSGRVDPTGKSLTADLDLIE 75

Query: 74  SSSVYGSDLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSPPESHVD 133
            SSVYG D+  L+LQAS ETKDRLR+RITDS RERWEIPD IIPRRSNSR RS PE  V 
Sbjct: 76  KSSVYGPDVERLSLQASFETKDRLRVRITDSNRERWEIPDEIIPRRSNSRIRSLPERDVG 135

Query: 134 SPATASISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQL 193
           SP T+ ISDPASDL FSL +T PFGFSV RRSSGDVLFDT+P+ SD  TFLVFKDQYIQL
Sbjct: 136 SPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDLETFLVFKDQYIQL 195

Query: 194 SSSLPKDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRS 253
           SSSLPKDRSSLFGIGEQTR+SFKLV +KSKTLTLWNADIG    DVNLYG+HPFYIDVRS
Sbjct: 196 SSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRS 255

Query: 254 PSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTE 313
           PSRDGKV AGTTHGVLLLNSNGMDIIY+GDRI+YK IGG+IDLYFFAGPSP+SVIDQYTE
Sbjct: 256 PSRDGKVAAGTTHGVLLLNSNGMDIIYTGDRISYKAIGGIIDLYFFAGPSPISVIDQYTE 315

Query: 314 LIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTF 373
           LIGRPAPVPYWSFG+HQCRYGYKNVSD+E VV+RYAK GIPLEVMWTDIDYMDGYKDFTF
Sbjct: 316 LIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVSRYAKAGIPLEVMWTDIDYMDGYKDFTF 375

Query: 374 DPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYSG 433
           DPINFP EKMK FVDNLHQNGQKYV ILDPGIS NKTYGT+IRG +ADIFIKY+GVPY G
Sbjct: 376 DPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLG 435

Query: 434 EVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDN 493
           EVW GPVYFPDF+HPNSEIFWG EIKLFR+IVPFDGLWIDMNEISN ITSSTS  SNLDN
Sbjct: 436 EVWPGPVYFPDFLHPNSEIFWGDEIKLFREIVPFDGLWIDMNEISNFITSSTSSFSNLDN 495

Query: 494 PPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFV 553
           PPYKINNA V RPINN TVPASSLHFGNLTEYNTHNLYG LES+ATHASLVKVTGKRPFV
Sbjct: 496 PPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFV 555

Query: 554 VSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEEL 613
           +SRSTFVGSGKYTAHWTGDN ATWNDLGYTIPSILNFGLFGIPMVG+DICGF+GDTTEEL
Sbjct: 556 LSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEEL 615

Query: 614 CRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHR 673
           CRRWIQLGAFYPFAR HSDK  IR+ELYLWD+VAA+ARKVLALRY LLPY YTLMYEAH+
Sbjct: 616 CRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHK 675

Query: 674 KGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNY 733
           KGTPIARPLFFSFPQDI+TYEI+SQFLLG GVLISPVLKEGA+SVDAYFP GNWFSLFNY
Sbjct: 676 KGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNY 735

Query: 734 SESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSS 793
           SESV++KSGQQ+TLDAPADHINVHVREGNIL LHG+A TT+AAR T FKLLVVVSNGQSS
Sbjct: 736 SESVAIKSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTEFKLLVVVSNGQSS 795

Query: 794 DGEVFLDDGEVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTFI 853
            GEVFLDDGE +EMGEEGGNWSLVRF+ EAVGSKLLIKS+V+NGGFALSQK+IIDKVT +
Sbjct: 796 SGEVFLDDGEEMEMGEEGGNWSLVRFYGEAVGSKLLIKSQVINGGFALSQKMIIDKVTIV 855

Query: 854 GFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVDISALSIPIWEEFKLELT 904
           GFER + MGG GL ISKGANLNGNS I+KTYE+S KFVNV+IS LSIPIWEEF +E +
Sbjct: 856 GFERPKNMGGLGLDISKGANLNGNSGIRKTYEHSAKFVNVEISGLSIPIWEEFVMEFS 913

BLAST of MS013380 vs. NCBI nr
Match: XP_022927400.1 (alpha-glucosidase [Cucurbita moschata])

HSP 1 Score: 1519.6 bits (3933), Expect = 0.0e+00
Identity = 746/898 (83.07%), Postives = 798/898 (88.86%), Query Frame = 0

Query: 14  RSLFGFSLFI-VCFF-------FSLAEPQPSPVVGYGYRIRSAHVDPAGKSLTAGLALIR 73
           R L    LF+  CFF       F+  E + +  VGYGYR+RS  VDP GKSLTA L LI 
Sbjct: 16  RLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLID 75

Query: 74  SSSVYGSDLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSPPESHVD 133
            SSVYG D+  L+LQAS ETKDRLR+RITDS RERWE+PD IIPRRSNSR RS PE  V 
Sbjct: 76  KSSVYGPDVERLSLQASFETKDRLRVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVG 135

Query: 134 SPATASISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQL 193
           SP T+ ISDPASDL FSL +T PFGFSV RRSSGDVLFDT+P+ SD  TFLVFKDQYIQL
Sbjct: 136 SPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQL 195

Query: 194 SSSLPKDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRS 253
           SSSLPKDRSSLFGIGEQTR+SFKLV +KSKTLTLWNADIG    DVNLYG+HPFYIDVRS
Sbjct: 196 SSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRS 255

Query: 254 PSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTE 313
           PSRDGKV AGTTHGVLLLNSNGMDIIY+GDRI+YK IGG+IDLYFFAGPSP+SVIDQYTE
Sbjct: 256 PSRDGKVAAGTTHGVLLLNSNGMDIIYTGDRISYKAIGGIIDLYFFAGPSPISVIDQYTE 315

Query: 314 LIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTF 373
           LIGRPAPVPYWSFG+HQCRYGYKNVSD+E VVARYAK GIPLEVMWTDIDYMDGYKDFTF
Sbjct: 316 LIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTF 375

Query: 374 DPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYSG 433
           DPINFP EKMK FVDNLHQNGQKYV ILDPGIS NKTYGT+IRG +ADIFIKY+GVPY G
Sbjct: 376 DPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLG 435

Query: 434 EVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDN 493
           EVW GPVYFPDF+HPNSEIFWG EIKL RDIVPFDGLWIDMNEISN ITSSTS  SNLDN
Sbjct: 436 EVWPGPVYFPDFLHPNSEIFWGDEIKLLRDIVPFDGLWIDMNEISNFITSSTSSFSNLDN 495

Query: 494 PPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFV 553
           PPYKINNA V RPINN TVPASSLHFGNLTEYNTHNLYG LES+ATHASLVKVTGKRPFV
Sbjct: 496 PPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFV 555

Query: 554 VSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEEL 613
           +SRSTFVGSGKYTAHWTGDN ATWNDLGYTIPSILNFGLFGIPMVG+DICGF+GDTTEEL
Sbjct: 556 LSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEEL 615

Query: 614 CRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHR 673
           CRRWIQLGAFYPFAR HSDK  IR+ELYLWD+VAA+ARKVLALRY LLPY YTLMYEAH+
Sbjct: 616 CRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHK 675

Query: 674 KGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNY 733
           KGTPIARPLFFSFPQDI+TYEI+SQFLLG GVLISPVLKEGA+SVDAYFP GNWFSLFNY
Sbjct: 676 KGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNY 735

Query: 734 SESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSS 793
           SESV++KSGQQ+TLDAPADHINVHVREGNIL LHG+A TT+AAR T FKLLVVVSNGQSS
Sbjct: 736 SESVAIKSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTEFKLLVVVSNGQSS 795

Query: 794 DGEVFLDDGEVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTFI 853
            GEVFLDDGE +EMGEEGGNWSLVRF+SEAVGSKLLIKS+V+NGGFALSQK+IIDKVT +
Sbjct: 796 SGEVFLDDGEEMEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIV 855

Query: 854 GFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVDISALSIPIWEEFKLELT 904
           GFER + MG  GL ISKGANLNGNS I+ TYEYS KFVNV IS LSIPI E F +EL+
Sbjct: 856 GFERPKNMGDLGLDISKGANLNGNSGIRITYEYSAKFVNVQISGLSIPICEGFVMELS 913

BLAST of MS013380 vs. ExPASy Swiss-Prot
Match: O04893 (Alpha-glucosidase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 532/884 (60.18%), Postives = 668/884 (75.57%), Query Frame = 0

Query: 29  SLAEPQPSPVVGYGYRIRSAHVDP-AGKSLTAGLALIRSSSVYGSDLHTLTLQASLETKD 88
           S +E  P  V+GYGY+++S  VD    +SLTA   L+++SSVYG D+  L++ ASLE+ D
Sbjct: 26  STSENDPEGVIGYGYKVKSVKVDSGTRRSLTALPQLVKNSSVYGPDIQLLSITASLESND 85

Query: 89  RLRIRITDSARERWEIPDNII---------PRRSNSRFRSPPESHVDSPATASISDPASD 148
           RLR+RITD+   RWEIPDNI+         P   +S +R+   S   +     +S P SD
Sbjct: 86  RLRVRITDAKHRRWEIPDNILHRHQPPPPPPHSLSSLYRTLLSSPTTNRRKILLSHPNSD 145

Query: 149 LAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRSSLFG 208
           L FSL NTTPFGF++ R+S+ DVLFD  PD ++P TFL+F DQY+ L+SSLP  R+ ++G
Sbjct: 146 LTFSLINTTPFGFTISRKSTHDVLFDATPDPTNPNTFLIFIDQYLHLTSSLPGTRAHIYG 205

Query: 209 IGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRSPSRDGKVVAGTTH 268
           +GE ++ +F+L  N  +TLT+  ADI  +  DVNLYGSHPFY+DVRS       VAG+TH
Sbjct: 206 LGEHSKPTFQLAHN--QTLTMRAADIPSSNPDVNLYGSHPFYMDVRS-----SPVAGSTH 265

Query: 269 GVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVPYWSF 328
           GVLLLNSNGMD+ Y+G+RITYKVIGG+IDLYFFAGPSP  V++Q+T +IGRPAP+PYW+F
Sbjct: 266 GVLLLNSNGMDVEYTGNRITYKVIGGIIDLYFFAGPSPGQVVEQFTRVIGRPAPMPYWAF 325

Query: 329 GYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTFDPINFPAEKMKIF 388
           G+ QCRYGY +V ++++VVA YAK  IPLEVMWTDIDYMD YKDFT DP+NFP +KMK F
Sbjct: 326 GFQQCRYGYHDVYELQSVVAGYAKAKIPLEVMWTDIDYMDAYKDFTLDPVNFPLDKMKKF 385

Query: 389 VDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYSGEVWSGPVYFPDFV 448
           V+NLH+NGQKYV ILDPGIS NKTY T+IRG K D+F+K NG PY G VW GPVYFPDF+
Sbjct: 386 VNNLHKNGQKYVVILDPGISTNKTYETYIRGMKHDVFLKRNGKPYLGSVWPGPVYFPDFL 445

Query: 449 HPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLRP 508
            P++  FW  EIK F +++P DGLWIDMNEISN I+S   P S LDNPPYKINN+ V+ P
Sbjct: 446 KPSALTFWTDEIKRFLNLLPVDGLWIDMNEISNFISSPPIPGSTLDNPPYKINNSGVMLP 505

Query: 509 INNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVVSRSTFVGSGKYT 568
           I N T+P +++H+G++ EYN HNL+G LE+R T A+L+K+T KRPFV+SRSTF GSGKYT
Sbjct: 506 IINKTIPPTAMHYGDIPEYNVHNLFGYLEARVTRAALIKLTEKRPFVLSRSTFSGSGKYT 565

Query: 569 AHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEELCRRWIQLGAFYPF 628
           AHWTGDN ATWNDL Y+IPS+L+FGLFGIPMVG+DICGF G+TTEELCRRWIQLGAFYPF
Sbjct: 566 AHWTGDNAATWNDLVYSIPSMLDFGLFGIPMVGADICGFLGNTTEELCRRWIQLGAFYPF 625

Query: 629 ARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHRKGTPIARPLFFSF 688
           +R HS      +ELY W+SVAA+ARKVL LRY LLPYFYTLMYEA   G PIARPLFFSF
Sbjct: 626 SRDHSSLGTTYQELYRWESVAASARKVLGLRYTLLPYFYTLMYEAQLNGIPIARPLFFSF 685

Query: 689 PQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNYSESVSVKSGQQVT 748
           P DIKTY ISSQFLLGKGV++SPVLK G +SV AYFP GNWF LF+Y+ SV+  +G+ VT
Sbjct: 686 PDDIKTYGISSQFLLGKGVMVSPVLKPGVVSVTAYFPRGNWFDLFDYTRSVTASTGRYVT 745

Query: 749 LDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSSDGEVFLDDGEVVE 808
           L AP DHINVH++EGNIL + G AMTT+AAR T F LLVV+S+  +S GE+FLDDG  V 
Sbjct: 746 LSAPPDHINVHIQEGNILAMQGKAMTTQAARKTPFHLLVVMSDCGASFGELFLDDGVEVT 805

Query: 809 MGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTFIGFERHEKMGGFGL 868
           MG   G W+ V+F + +     +I S+VV+G FA+SQK +IDKVT +G  +  K+ G+ +
Sbjct: 806 MGVNRGKWTFVKFIAASAKQTCIITSDVVSGEFAVSQKWVIDKVTILGLRKGTKINGYTV 865

Query: 869 KISKGANLNGNSAIKKTYEYSGKFVNVDISALSIPIWEEFKLEL 903
           +          S +K T +  G+F+  +IS L++ +  EFKL L
Sbjct: 866 RTGAVTRKGDKSKLKSTPDRKGEFIVAEISGLNLLLGREFKLVL 902

BLAST of MS013380 vs. ExPASy Swiss-Prot
Match: O04931 (Alpha-glucosidase OS=Beta vulgaris OX=161934 PE=1 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 5.1e-306
Identity = 509/874 (58.24%), Postives = 662/874 (75.74%), Query Frame = 0

Query: 39  VGYGYRIRSAHVD-PAGKSLTAGLALIRSSSVYGSDLHTLTLQASLETKDRLRIRITDSA 98
           +GYGY++++A VD   GKSLTA L LIR+S VYG D+H L+  AS E  D LRIR TD+ 
Sbjct: 41  IGYGYQVKNAKVDNSTGKSLTALLQLIRNSPVYGPDIHFLSFTASFEEDDTLRIRFTDAN 100

Query: 99  RERWEIPDNIIPRRS--------NSRFRSPPESHVDSPATASISDPASDLAFSLRNTTPF 158
             RWEIP+ ++PR          +S    P     + P T  +S P SDLAF+L +TTPF
Sbjct: 101 NRRWEIPNEVLPRPPPPPSPPPLSSLQHLPKPIPQNQPTTTVLSHPHSDLAFTLFHTTPF 160

Query: 159 GFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRESFKL 218
           GF++ R+S+ DVLFD  P  S+P TFL++KDQY+QLSSSLP  ++ L+G+GE T+ +F+L
Sbjct: 161 GFTIYRKSTHDVLFDATPIPSNPTTFLIYKDQYLQLSSSLPAQQAHLYGLGEHTKPTFQL 220

Query: 219 VSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRSPSRDGKVVAGTTHGVLLLNSNGMD 278
             N  + LTLWNADI     D+NLYGSHPFY+DVRS       + G+THGV LLNSNGMD
Sbjct: 221 AHN--QILTLWNADIASFNRDLNLYGSHPFYMDVRS-----SPMVGSTHGVFLLNSNGMD 280

Query: 279 IIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVPYWSFGYHQCRYGYKN 338
           + Y+GDRITYKVIGG+IDLY FAG +P  V+DQYT+LIGRPAP+PYW+FG+HQCR+GY++
Sbjct: 281 VEYTGDRITYKVIGGIIDLYIFAGRTPEMVLDQYTKLIGRPAPMPYWAFGFHQCRWGYRD 340

Query: 339 VSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTFDPINFPAEKMKIFVDNLHQNGQKY 398
           V+++E VV +YA+  IPLEVMWTDIDYMD +KDFT DP++FP +KM+ FV  LH+NGQ+Y
Sbjct: 341 VNEIETVVDKYAEARIPLEVMWTDIDYMDAFKDFTLDPVHFPLDKMQQFVTKLHRNGQRY 400

Query: 399 VAILDPGISINKTYGTFIRGTKADIFIKYNGVPYSGEVWSGPVYFPDFVHPNSEIFWGGE 458
           V ILDPGI+ NK+YGTFIRG ++++FIK NG PY G VW GPVY+PDF+ P +  FW  E
Sbjct: 401 VPILDPGINTNKSYGTFIRGMQSNVFIKRNGNPYLGSVWPGPVYYPDFLDPAARSFWVDE 460

Query: 459 IKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLRPINNNTVPASSL 518
           IK FRDI+P DG+WIDMNE SN ITS+ +P S LDNPPYKINN+    PIN+ T+PA+++
Sbjct: 461 IKRFRDILPIDGIWIDMNEASNFITSAPTPGSTLDNPPYKINNSGGRVPINSKTIPATAM 520

Query: 519 HFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVVSRSTFVGSGKYTAHWTGDNDATW 578
           H+GN+TEYN HNLYG LES+AT  +LV+   + PF++SRSTF GSGKYTAHWTGDN A W
Sbjct: 521 HYGNVTEYNAHNLYGFLESQATREALVRPATRGPFLLSRSTFAGSGKYTAHWTGDNAARW 580

Query: 579 NDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEELCRRWIQLGAFYPFARGHSDKFYIR 638
           +DL Y+IP++LNFGLFG+PM+G+DICGFA  TTEELC RWIQLGAFYPF+R HS +    
Sbjct: 581 DDLQYSIPTMLNFGLFGMPMIGADICGFAESTTEELCCRWIQLGAFYPFSRDHSARDTTH 640

Query: 639 RELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHRKGTPIARPLFFSFPQDIKTYEISS 698
           +ELYLW+SVAA+AR VL LRY LLPY+YTLMY+A+ +G+PIARPL F+FP D+ TY ISS
Sbjct: 641 QELYLWESVAASARTVLGLRYELLPYYYTLMYDANLRGSPIARPLSFTFPDDVATYGISS 700

Query: 699 QFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNYSESVSVKSGQQVTLDAPADHINVH 758
           QFL+G+G+++SPVL+ G+  V+AY P GNW SL NY+ SVSV +G  V+L AP DHINVH
Sbjct: 701 QFLIGRGIMVSPVLQPGSSIVNAYSPRGNWVSLSNYTSSVSVSAGTYVSLSAPPDHINVH 760

Query: 759 VREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSSDGEVFLDDGEVVEMGEEGGNWSLV 818
           + EGNI+ + G+AMTT+AAR T F LLVV+S+  +S GE+FLD+G  +++G  GG W+LV
Sbjct: 761 IHEGNIVAMQGEAMTTQAARSTPFHLLVVMSDHVASTGELFLDNGIEMDIGGPGGKWTLV 820

Query: 819 RFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTFIGFERHEKMGGFGLKISKGA-NLNG 878
           RF +E+  + L I SEVVN G+A+SQ+ ++DK+T +G +R  K+  + ++   GA  + G
Sbjct: 821 RFFAESGINNLTISSEVVNRGYAMSQRWVMDKITILGLKRRVKIKEYTVQKDAGAIKVKG 880

Query: 879 NSAIKKTYEYSGKFVNVDISALSIPIWEEFKLEL 903
                 ++   G FV+V IS L   + + FKLEL
Sbjct: 881 LGRRTSSHNQGGFFVSV-ISDLRQLVGQAFKLEL 906

BLAST of MS013380 vs. ExPASy Swiss-Prot
Match: Q653V7 (Probable alpha-glucosidase Os06g0675700 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0675700 PE=1 SV=1)

HSP 1 Score: 974.2 bits (2517), Expect = 1.0e-282
Identity = 495/901 (54.94%), Postives = 642/901 (71.25%), Query Frame = 0

Query: 14  RSLFGFSLFIVCFFFSLAEPQPSPVVGYGYRIRSAHVDPAGKSLTAGLALI----RSSSV 73
           R L   ++F++  F  LA P     V  GY +  A V  +   L A L L      ++  
Sbjct: 11  RRLGALAVFLLALF--LAAPWG---VDCGYNV--ASVAGSKNRLRARLELAGGGGGAAPE 70

Query: 74  YGSDLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRS---PPESHVDS 133
            G D+  L+L ASLET  RL +RITD+   RWE+P ++IPR S   F +   P    V S
Sbjct: 71  LGPDVRRLSLTASLETDSRLHVRITDADHPRWEVPQDVIPRPSPDSFLAATRPGGGRVLS 130

Query: 134 PATASISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLS 193
            AT       SDL F++ +T+PF F+V RRS+GDVLFDT P+       LVFKD+Y++L+
Sbjct: 131 TAT-------SDLTFAI-HTSPFRFTVTRRSTGDVLFDTTPN-------LVFKDRYLELT 190

Query: 194 SSL-PKDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRS 253
           SSL P  R+SL+G+GEQT+ +F+L   ++ T TLWN+DI    VD+NLYGSHPFY+DVRS
Sbjct: 191 SSLPPPGRASLYGLGEQTKRTFRL--QRNDTFTLWNSDIAAGNVDLNLYGSHPFYMDVRS 250

Query: 254 PSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTE 313
               G    G  HGVLLLNSNGMD+IY G  +TYKVIGGV+D YFFAGPSP++V+DQYT+
Sbjct: 251 GGGGG---GGAAHGVLLLNSNGMDVIYGGSYVTYKVIGGVLDFYFFAGPSPLAVVDQYTQ 310

Query: 314 LIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTF 373
           LIGRPAP+PYWSFG+HQCRYGYKNV+D+E VVA YAK  IPLEVMWTDIDYMD YKDFT 
Sbjct: 311 LIGRPAPMPYWSFGFHQCRYGYKNVADLEGVVAGYAKARIPLEVMWTDIDYMDAYKDFTL 370

Query: 374 DPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYSG 433
           DP+NFPA++M+ FVD LH+NGQK+V I+DPGI++N TYGTF+RG K DIF+K+NG  Y G
Sbjct: 371 DPVNFPADRMRPFVDRLHRNGQKFVVIIDPGINVNTTYGTFVRGMKQDIFLKWNGSNYLG 430

Query: 434 EVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDN 493
            VW G VYFPDF++P +  FW  EI  FR  +P DGLW+DMNEISN +     PL+ +D+
Sbjct: 431 VVWPGNVYFPDFLNPRAAEFWAREIAAFRRTLPVDGLWVDMNEISNFV--DPPPLNAIDD 490

Query: 494 PPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFV 553
           PPY+INN+ V RPINN TVPAS++H+G + EY+ HNL+G LE+RATH +L++ TG+RPFV
Sbjct: 491 PPYRINNSGVRRPINNKTVPASAVHYGGVAEYDAHNLFGFLEARATHDALLRDTGRRPFV 550

Query: 554 VSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEEL 613
           +SRSTFVGSG+YTAHWTGDN ATW DL Y+I ++L+FGLFGIPM+G+DICGF G+TTEEL
Sbjct: 551 LSRSTFVGSGRYTAHWTGDNAATWEDLHYSINTMLSFGLFGIPMIGADICGFGGNTTEEL 610

Query: 614 CRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHR 673
           C RWIQLGAFYPF+R HS    +RRELYLW+SVA +ARK L LRYRLLPY YTLMYEAH 
Sbjct: 611 CSRWIQLGAFYPFSRDHSAIGTVRRELYLWESVARSARKALGLRYRLLPYLYTLMYEAHT 670

Query: 674 KGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNY 733
            G PIARPLFFS+P D++TY I  QFLLG+GVL+SPVL+ GA +V AYFP G WFSL+++
Sbjct: 671 TGAPIARPLFFSYPGDVETYGIDRQFLLGRGVLVSPVLEPGATTVTAYFPAGRWFSLYDF 730

Query: 734 SESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSS 793
           S +V+ K+G++VTL APAD +NVHV  GNIL L   A+T+   R +   LLV +++  ++
Sbjct: 731 SLAVATKTGKRVTLPAPADTVNVHVAGGNILTLQQPALTSSRVRQSVVHLLVALADDGTA 790

Query: 794 DGEVFLDDGEVVEMGEEGGNWSLVRFH--SEAVGSKLLIKSEVVNGGFALSQKLIIDKVT 853
            G++FLDDGE  EM      WS ++F   +E+ G  + ++S VV+  +A S+ + I KV 
Sbjct: 791 TGDLFLDDGESPEMAGPRSRWSQIKFSGATESGGGVVRVRSHVVHDSYAPSRTMAIGKVV 850

Query: 854 FIGFERHEKMGGFGLKISKGANLNGNSAI--KKTYEYSGKFVNVDISALSIPIWEEFKLE 903
            +G        GF +  + G  +N ++A+         G      +S L++ + +EF L+
Sbjct: 851 LMGLRSAAPPKGFAV-YANGVQVNASTAVGGAAGSPEKGALGVAHVSGLTLVVGQEFDLK 881

BLAST of MS013380 vs. ExPASy Swiss-Prot
Match: Q43763 (Alpha-glucosidase OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 2.0e-273
Identity = 471/839 (56.14%), Postives = 609/839 (72.59%), Query Frame = 0

Query: 73  DLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRS--NSRFRSPPESHVDSPATA 132
           D+  L + ASLET  RLR+RITD+   RWE+P +IIPR +  +    +PP S   +P   
Sbjct: 59  DVQRLAVYASLETDSRLRVRITDADHPRWEVPQDIIPRPAPGDVLHDAPPAS--SAPLQG 118

Query: 133 SISDPA-SDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSL 192
            +  PA SDL  ++ + +PF F+V RRS+GD LFDTAP        LVF+D+Y++++S+L
Sbjct: 119 RVLSPAGSDLVLTV-HASPFRFTVSRRSTGDTLFDTAPG-------LVFRDKYLEVTSAL 178

Query: 193 PKDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRSPSRD 252
           P  R+SL+G+GE T+ SF+L  N S   TLWNADIG + VDVNLYGSHPFY+DVR+P   
Sbjct: 179 PAGRASLYGLGEHTKSSFRLRHNDS--FTLWNADIGASYVDVNLYGSHPFYMDVRAP--- 238

Query: 253 GKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGR 312
                GT HGVLLL+SNGMD++Y G  +TYKVIGGV+D YFFAGP+P++V+DQYT+LI R
Sbjct: 239 -----GTAHGVLLLSSNGMDVLYGGSYVTYKVIGGVLDFYFFAGPNPLAVVDQYTQLIAR 298

Query: 313 PAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTFDPIN 372
           PAP+PYWSFG+HQCRYGY NVSD+E VVARYAK  IPLEVMWTDIDYMDG+KDFT D +N
Sbjct: 299 PAPMPYWSFGFHQCRYGYLNVSDLERVVARYAKARIPLEVMWTDIDYMDGFKDFTLDRVN 358

Query: 373 FPAEKMKIFVDNLHQNGQKYVAILDPGI---SINKTYGTFIRGTKADIFIKYNGVPYSGE 432
           F A +++ FVD LH+N QKYV ILDPGI    I+ TYGTF+RG + DIF+K NG  + G 
Sbjct: 359 FTAAELRPFVDRLHRNAQKYVLILDPGIRVDPIDATYGTFVRGMQQDIFLKRNGTNFVGN 418

Query: 433 VWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDNP 492
           VW G VYFPDF+HP +  FW  EI LFR  +P DGLWIDMNEISN    +  P++ LD+P
Sbjct: 419 VWPGDVYFPDFMHPAAAEFWAREISLFRRTIPVDGLWIDMNEISNFY--NPEPMNALDDP 478

Query: 493 PYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVV 552
           PY+INN    RPINN TV   ++H+G +TEY  HNL+GLLE+RAT   +++ TG+RPFV+
Sbjct: 479 PYRINNDGTGRPINNKTVRPLAVHYGGVTEYEEHNLFGLLEARATGRGVLRDTGRRPFVL 538

Query: 553 SRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEELC 612
           SRSTFVGSG+YTA+WTGDN ATW DL Y+I ++L+FGLFG+PM+G+DICGF G+TTEELC
Sbjct: 539 SRSTFVGSGRYTAYWTGDNAATWGDLRYSINTMLSFGLFGMPMIGADICGFNGNTTEELC 598

Query: 613 RRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHRK 672
            RWIQLGAFYPF+R HS  F +RRELYLW SVAA+ RK L LRY+LLPYFYTLMYEAH  
Sbjct: 599 GRWIQLGAFYPFSRDHSAIFTVRRELYLWPSVAASGRKALGLRYQLLPYFYTLMYEAHMT 658

Query: 673 GTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNYS 732
           G PIARPLFFS+P D+ TY +  QFLLG+GVL+SPVL+ G  +VDAYFP G W+ L++YS
Sbjct: 659 GAPIARPLFFSYPHDVATYGVDRQFLLGRGVLVSPVLEPGPTTVDAYFPAGRWYRLYDYS 718

Query: 733 ESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSSD 792
            +V+ ++G+ V L APAD +NVH+  G IL L   A+TT  AR TAF LLV ++   ++ 
Sbjct: 719 LAVATRTGKHVRLPAPADTVNVHLTGGTILPLQQSALTTSRARRTAFHLLVALAEDGTAS 778

Query: 793 GEVFLDDGEVVEMGEEGGNWSLVRFHSEAVGSK--LLIKSEVVNGGFALSQKLIIDKVTF 852
           G +FLDDG+  E G    +WS+VRF+ +   +K  + +KSEVV+  +A S+ L+I KV  
Sbjct: 779 GYLFLDDGDSPEYGRR-SDWSMVRFNYKIPNNKGAIKVKSEVVHNSYAQSRTLVISKVVL 838

Query: 853 IGFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNV-DISALSIPIWEEFKLEL 903
           +G           + ++  A +  +S+    Y+ +G    V  I  LS+ + EEF+L++
Sbjct: 839 MGHRSPAAPKKLTVHVN-SAEVEASSSAGTRYQNAGGLGGVAHIGGLSLVVGEEFELKV 873

BLAST of MS013380 vs. ExPASy Swiss-Prot
Match: Q9S7Y7 (Alpha-xylosidase 1 OS=Arabidopsis thaliana OX=3702 GN=XYL1 PE=1 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 1.1e-228
Identity = 412/930 (44.30%), Postives = 580/930 (62.37%), Query Frame = 0

Query: 8   MAKTATRSLFGFSL---FIVCFFFSLAEPQPSPVVGYGYRIRSAHVDPAGKSLTAGLALI 67
           MA +++   F  SL    I+CF    +  Q    +G GYR+ S    P G      L + 
Sbjct: 1   MASSSSSLAFSLSLLLALILCF----SPTQSYKTIGKGYRLVSIEESPDG-GFIGYLQVK 60

Query: 68  RSSSVYGSDLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSPPESHV 127
           + + +YGSD+ TL L    ET  RLR+ ITD+ ++RWE+P N++PR    +         
Sbjct: 61  QKNKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPREQPPQVGKVIGKSR 120

Query: 128 DSPATASISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ 187
            SP T       S+L FS   T PF F+V RRS+ + LF+T        + LVFKDQY++
Sbjct: 121 KSPITVQ-EISGSELIFSY-TTDPFTFAVKRRSNHETLFNTT-------SSLVFKDQYLE 180

Query: 188 LSSSLPKDRSSLFGIGEQTR-ESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDV 247
           +S+SLPK+ +SL+G+GE ++    KLV N  +  TL+  D+    ++ +LYGSHP Y+D+
Sbjct: 181 ISTSLPKE-ASLYGLGENSQANGIKLVPN--EPYTLYTEDVSAINLNTDLYGSHPMYMDL 240

Query: 248 RSPSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQY 307
           R+    GK  A   H VLLLNSNGMD+ Y GD +TYKVIGGV D YF AGPSP++V+DQY
Sbjct: 241 RNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIAGPSPLNVVDQY 300

Query: 308 TELIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDF 367
           T+LIGRPAP+PYWS G+HQCR+GY N+S VE+VV  Y K  IPL+V+W D D+MDG+KDF
Sbjct: 301 TQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDVIWNDDDHMDGHKDF 360

Query: 368 TFDPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPY 427
           T +P+ +P  K+  F+D +H+ G KY+ I DPGI +N +YGTF R   AD+FIKY G P+
Sbjct: 361 TLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRAMAADVFIKYEGKPF 420

Query: 428 SGEVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISN------------ 487
             +VW GPVYFPDF++P +  +WG EIK F D+VP DGLWIDMNE+SN            
Sbjct: 421 LAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEVSNFCSGLCTIPEGK 480

Query: 488 ------------AITSSTSPLSNLDNPPYKINNAAVLRPINNNTVPASSLHFGNLTEYNT 547
                        +       +  D+PPYKIN   V+ P+   T+  S+ H+  + EY+ 
Sbjct: 481 QCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIATSATHYNGVREYDA 540

Query: 548 HNLYGLLESRATHASLVKVTGKRPFVVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSI 607
           H++YG  E+ ATH  L+ V GKRPF++SRSTFVGSG+Y AHWTGDN  TW  L  +I ++
Sbjct: 541 HSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVSISTM 600

Query: 608 LNFGLFGIPMVGSDICGFAGDTTEELCRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVA 667
           LNFG+FG+PMVGSDICGF    TEELC RWI++GAFYPF+R H++ +  R+ELY WD+VA
Sbjct: 601 LNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQWDTVA 660

Query: 668 ATARKVLALRYRLLPYFYTLMYEAHRKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLI 727
            +AR  L +RY++LP+ YTL YEAH  G PIARPLFFSFP+  + Y  S QFLLG   +I
Sbjct: 661 DSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTECYGNSRQFLLGSSFMI 720

Query: 728 SPVLKEGALSVDAYFPEGNWFSLFNYSESVSVKSGQQVTLDAPADHINVHVREGNILVLH 787
           SPVL++G   V+A FP G+W+ +F+ +++V  K+G++VTL AP + +NVH+ +  IL   
Sbjct: 721 SPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNFVNVHLYQNTILPTQ 780

Query: 788 GDAMTTRAARGTAFKLLVVVSNGQS---SDGEVFLDDGEVVEMGEEGGNWSLVRFHSEAV 847
              + ++ AR T F L++    G S   + G+++LD+ E+ EM    G  + V F++   
Sbjct: 781 QGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMKLGNGQSTYVDFYASVG 840

Query: 848 GSKLLIKSEVVNGGFALSQKLIIDKVTFIGFERHEKMGGFGLKISKGANLNGNSAIKKTY 901
              + + S+V  G FALS+  +I+KV+ +G     ++    +  S        S+ + TY
Sbjct: 841 NGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQINGSPMTKKIEVSSKEHTY 900

BLAST of MS013380 vs. ExPASy TrEMBL
Match: A0A6J1CNJ7 (alpha-glucosidase-like OS=Momordica charantia OX=3673 GN=LOC111012738 PE=3 SV=1)

HSP 1 Score: 1735.3 bits (4493), Expect = 0.0e+00
Identity = 862/899 (95.88%), Postives = 871/899 (96.89%), Query Frame = 0

Query: 8   MAKTATRSLFGFSLFI-VCFFF-SLAEPQPSPVVGYGYRIRSAHVDPAGKSLTAGLALI- 67
           M +TATR+  G S FI +CFFF  LA+P P P  G+GYRIRS  + P GKSLTA LALI 
Sbjct: 27  MQRTATRATSGLSFFILICFFFLCLAQPPPPPPAGHGYRIRSVQLHPTGKSLTADLALIG 86

Query: 68  RSSSVYGSDLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSPPESHV 127
            SSSVYG DL TLTLQAS ETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRS PESHV
Sbjct: 87  TSSSVYGPDLPTLTLQASFETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSLPESHV 146

Query: 128 DSPATASISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ 187
           DSPATA ISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ
Sbjct: 147 DSPATAFISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ 206

Query: 188 LSSSLPKDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVR 247
           LSS LP DRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVR
Sbjct: 207 LSSLLPNDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVR 266

Query: 248 SPSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYT 307
           SPSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYT
Sbjct: 267 SPSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYT 326

Query: 308 ELIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFT 367
           ELIGRPAPVPYWSFG+HQCRYGYKNVSDVENVVARYAK GIPLEVMWTDIDYMDGYKDFT
Sbjct: 327 ELIGRPAPVPYWSFGFHQCRYGYKNVSDVENVVARYAKAGIPLEVMWTDIDYMDGYKDFT 386

Query: 368 FDPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYS 427
           FDPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYS
Sbjct: 387 FDPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYS 446

Query: 428 GEVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLD 487
           GEVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLD
Sbjct: 447 GEVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLD 506

Query: 488 NPPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPF 547
           NPPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPF
Sbjct: 507 NPPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPF 566

Query: 548 VVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEE 607
           VVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEE
Sbjct: 567 VVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEE 626

Query: 608 LCRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAH 667
           LCRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAH
Sbjct: 627 LCRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAH 686

Query: 668 RKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFN 727
           RKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFN
Sbjct: 687 RKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFN 746

Query: 728 YSESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQS 787
           YSESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQS
Sbjct: 747 YSESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQS 806

Query: 788 SDGEVFLDDGEVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTF 847
           SDGEVFLDDG VVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFAL+QKLIIDKVTF
Sbjct: 807 SDGEVFLDDGGVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALTQKLIIDKVTF 866

Query: 848 IGFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVDISALSIPIWEEFKLELT 904
           IGFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNV+ISALSIPIWEEFKLELT
Sbjct: 867 IGFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVEISALSIPIWEEFKLELT 925

BLAST of MS013380 vs. ExPASy TrEMBL
Match: A0A6J1KFK1 (alpha-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111495351 PE=3 SV=1)

HSP 1 Score: 1541.9 bits (3991), Expect = 0.0e+00
Identity = 753/891 (84.51%), Postives = 808/891 (90.68%), Query Frame = 0

Query: 16  LFGFSLF---IVCFFFSLAEPQPSPVVGYGYRIRSAHVDPAGKSLTAGLALIRSSSVYGS 75
           LF FS F   I    F+  E + +  VGYGYR+RS  VDP GKSLTA L LI  SSVYG 
Sbjct: 44  LFLFSCFFRLIASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGP 103

Query: 76  DLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSPPESHVDSPATASI 135
           D+  L+LQAS ETKDRLR+RITDS RERWEIPD IIPRRSNSR RS PE  V SP T+ I
Sbjct: 104 DVERLSLQASFETKDRLRVRITDSNRERWEIPDEIIPRRSNSRIRSLPERDVGSPETSII 163

Query: 136 SDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKD 195
           SDPASDL FSL +T PFGFSV RRSSGDVLFDT+P+ SD  TFLVFKDQYIQLSSSLPKD
Sbjct: 164 SDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKD 223

Query: 196 RSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRSPSRDGKV 255
           RSSLFGIGEQTR+SFK+V +KSKTLTLW+ADIG    DVNLYG+HPFYIDVRSPSRDGKV
Sbjct: 224 RSSLFGIGEQTRKSFKIVPDKSKTLTLWSADIGSVNTDVNLYGAHPFYIDVRSPSRDGKV 283

Query: 256 VAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAP 315
            AGTTHGVLLLNSNGMDIIYSGDRI+YK IGG+IDLYFFAGPSP+SVIDQYTELIGRPAP
Sbjct: 284 AAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAP 343

Query: 316 VPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTFDPINFPA 375
           VPYWSFG+HQCRYGYKNVSD+E VVARYAK GIPLEVMWTDIDYMDGYKDFTFDPINFP 
Sbjct: 344 VPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPV 403

Query: 376 EKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYSGEVWSGPV 435
           EKMK FVD+LHQNGQKYV I+DPGIS NKTYGT+IRG +ADIFIKY+GVPY GEVW GPV
Sbjct: 404 EKMKNFVDSLHQNGQKYVLIVDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPV 463

Query: 436 YFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINN 495
           YFPDF+HPNSEIFWGGEIKLFRDIVPFDGLWIDMNE+SN ITSSTSP SNLDNPPYKI+N
Sbjct: 464 YFPDFLHPNSEIFWGGEIKLFRDIVPFDGLWIDMNELSNFITSSTSPFSNLDNPPYKIDN 523

Query: 496 AAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVVSRSTFV 555
           A V RPINN TVPASSLHFGNLTEYNTHNLYG LES+ATHASLVKVTGKRPFV+SRSTFV
Sbjct: 524 AGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFV 583

Query: 556 GSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEELCRRWIQL 615
           GSGKYTAHWTGDN ATWNDLGYTIPSILNFGLFGIPMVG+DICGF+GDTTEELCRRWIQL
Sbjct: 584 GSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELCRRWIQL 643

Query: 616 GAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHRKGTPIAR 675
           GAFYPFAR HSDK  IR+ELYLWD+VAA+ARKVLALRY LLPY YTLMYEAH+KGTPIAR
Sbjct: 644 GAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPIAR 703

Query: 676 PLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNYSESVSVK 735
           PLFFSFPQDI+TYEI+SQFLLG+GVL+SPVLKEGALSVDAYFP GNWFSLFNYSESV+VK
Sbjct: 704 PLFFSFPQDIRTYEINSQFLLGEGVLVSPVLKEGALSVDAYFPAGNWFSLFNYSESVAVK 763

Query: 736 SGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSSDGEVFLD 795
           SGQQ+TLDAPADHINVHVREGNIL LHG+A TT+AAR TAFKLLVVVSNGQSS GEVFLD
Sbjct: 764 SGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLD 823

Query: 796 DGEVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTFIGFERHEK 855
           DGEVVEMGEEGGNWSLVRF+SEAVGSKLLIKS+V+NGGFALSQK+IIDKVTF+GFER +K
Sbjct: 824 DGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTFVGFERPKK 883

Query: 856 MGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVDISALSIPIWEEFKLELT 904
           MGG GL ISKGANLNGNS I+KTYEYS KFVNV+IS LSIPI EEF +EL+
Sbjct: 884 MGGLGLDISKGANLNGNSGIRKTYEYSAKFVNVEISGLSIPICEEFVMELS 934

BLAST of MS013380 vs. ExPASy TrEMBL
Match: A0A6J1ENT6 (alpha-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111434234 PE=3 SV=1)

HSP 1 Score: 1519.6 bits (3933), Expect = 0.0e+00
Identity = 746/898 (83.07%), Postives = 798/898 (88.86%), Query Frame = 0

Query: 14  RSLFGFSLFI-VCFF-------FSLAEPQPSPVVGYGYRIRSAHVDPAGKSLTAGLALIR 73
           R L    LF+  CFF       F+  E + +  VGYGYR+RS  VDP GKSLTA L LI 
Sbjct: 16  RLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLID 75

Query: 74  SSSVYGSDLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSPPESHVD 133
            SSVYG D+  L+LQAS ETKDRLR+RITDS RERWE+PD IIPRRSNSR RS PE  V 
Sbjct: 76  KSSVYGPDVERLSLQASFETKDRLRVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVG 135

Query: 134 SPATASISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQL 193
           SP T+ ISDPASDL FSL +T PFGFSV RRSSGDVLFDT+P+ SD  TFLVFKDQYIQL
Sbjct: 136 SPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQL 195

Query: 194 SSSLPKDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRS 253
           SSSLPKDRSSLFGIGEQTR+SFKLV +KSKTLTLWNADIG    DVNLYG+HPFYIDVRS
Sbjct: 196 SSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRS 255

Query: 254 PSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTE 313
           PSRDGKV AGTTHGVLLLNSNGMDIIY+GDRI+YK IGG+IDLYFFAGPSP+SVIDQYTE
Sbjct: 256 PSRDGKVAAGTTHGVLLLNSNGMDIIYTGDRISYKAIGGIIDLYFFAGPSPISVIDQYTE 315

Query: 314 LIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTF 373
           LIGRPAPVPYWSFG+HQCRYGYKNVSD+E VVARYAK GIPLEVMWTDIDYMDGYKDFTF
Sbjct: 316 LIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTF 375

Query: 374 DPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYSG 433
           DPINFP EKMK FVDNLHQNGQKYV ILDPGIS NKTYGT+IRG +ADIFIKY+GVPY G
Sbjct: 376 DPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLG 435

Query: 434 EVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDN 493
           EVW GPVYFPDF+HPNSEIFWG EIKL RDIVPFDGLWIDMNEISN ITSSTS  SNLDN
Sbjct: 436 EVWPGPVYFPDFLHPNSEIFWGDEIKLLRDIVPFDGLWIDMNEISNFITSSTSSFSNLDN 495

Query: 494 PPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFV 553
           PPYKINNA V RPINN TVPASSLHFGNLTEYNTHNLYG LES+ATHASLVKVTGKRPFV
Sbjct: 496 PPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFV 555

Query: 554 VSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEEL 613
           +SRSTFVGSGKYTAHWTGDN ATWNDLGYTIPSILNFGLFGIPMVG+DICGF+GDTTEEL
Sbjct: 556 LSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEEL 615

Query: 614 CRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHR 673
           CRRWIQLGAFYPFAR HSDK  IR+ELYLWD+VAA+ARKVLALRY LLPY YTLMYEAH+
Sbjct: 616 CRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHK 675

Query: 674 KGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNY 733
           KGTPIARPLFFSFPQDI+TYEI+SQFLLG GVLISPVLKEGA+SVDAYFP GNWFSLFNY
Sbjct: 676 KGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNY 735

Query: 734 SESVSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSS 793
           SESV++KSGQQ+TLDAPADHINVHVREGNIL LHG+A TT+AAR T FKLLVVVSNGQSS
Sbjct: 736 SESVAIKSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTEFKLLVVVSNGQSS 795

Query: 794 DGEVFLDDGEVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTFI 853
            GEVFLDDGE +EMGEEGGNWSLVRF+SEAVGSKLLIKS+V+NGGFALSQK+IIDKVT +
Sbjct: 796 SGEVFLDDGEEMEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIV 855

Query: 854 GFERHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVDISALSIPIWEEFKLELT 904
           GFER + MG  GL ISKGANLNGNS I+ TYEYS KFVNV IS LSIPI E F +EL+
Sbjct: 856 GFERPKNMGDLGLDISKGANLNGNSGIRITYEYSAKFVNVQISGLSIPICEGFVMELS 913

BLAST of MS013380 vs. ExPASy TrEMBL
Match: A0A5D3E1Q3 (Alpha-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00330 PE=3 SV=1)

HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 735/895 (82.12%), Postives = 805/895 (89.94%), Query Frame = 0

Query: 14  RSLFGFSLFIVCFFF--SLAEPQPS---PVVGYGYRIRSAHVDPAGKSLTAGLALIRSSS 73
           R+ F  SL I+  F   S   P P+   P VG+GYRIRS+H+DPAGKSLTA L LIR+S 
Sbjct: 12  RTPFAHSLLILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPAGKSLTADLQLIRTSQ 71

Query: 74  VYGSDLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSPPESHVDSPA 133
           VYG DL TLTLQA+ E+KDRLR+RITDS RERWEIPD+IIPR SNS  RS PE+HV SP 
Sbjct: 72  VYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSNSLIRSLPENHVASPK 131

Query: 134 TASISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSS 193
            + ISDPASDL F+L +T PFGFSVLRRSSGDVLFDT+PD S+  TF+VFKDQYIQLSS 
Sbjct: 132 PSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSETFIVFKDQYIQLSSL 191

Query: 194 LPKDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRSPSR 253
           LPKDRSS+FGIGEQTR+SFKLV +K+KTLTLWNADIG   +DVNLYG+HPFYIDVRS SR
Sbjct: 192 LPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDVRSRSR 251

Query: 254 DGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIG 313
           DGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGG+IDLYFFAGPSP+SVIDQYTELIG
Sbjct: 252 DGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIG 311

Query: 314 RPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTFDPI 373
           RPAPVPYWSFG+HQCRYGYKNVS+VE+VVARYAK  IPLEVMWTDIDYMDGYKDFTFDPI
Sbjct: 312 RPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEVMWTDIDYMDGYKDFTFDPI 371

Query: 374 NFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYSGEVW 433
           NFP+EKMKIFVDNLH+NGQKYV ILDPGIS N TYG +IRGTKADIF+K++GVPY G+VW
Sbjct: 372 NFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRGTKADIFMKHDGVPYLGDVW 431

Query: 434 SGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDNPPY 493
            GPVYFPDF+HP+SE FWG EI++FRDIVPFDGLWIDMNEISN ITSSTSPLSNLDNPPY
Sbjct: 432 PGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNLDNPPY 491

Query: 494 KINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVVSR 553
            INNA V RP+NN TVPAS LHFGNLTEYNTHNLYG LESRATHASLVKVTGKRPFV+SR
Sbjct: 492 MINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTGKRPFVLSR 551

Query: 554 STFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEELCRR 613
           STF GSGKYTAHWTGD  ATWND+GYTIPSILNFGLFGIPMVGSDICGF+GDTTEELCRR
Sbjct: 552 STFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRR 611

Query: 614 WIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHRKGT 673
           WIQLGAFYPFAR HSDK  IR+ELYLWDSVAA+ARKVLALRY+LLPYFYTLMYEAH+KGT
Sbjct: 612 WIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGT 671

Query: 674 PIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNYSES 733
           PIARPLFFSFPQDIKTYEI SQFLLG+GVL+SPVLKEGA SVDAYFP GNWFSLFNYSE 
Sbjct: 672 PIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEF 731

Query: 734 VSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSSDGE 793
           V+V SGQQ+ LDAPADHINVHVREGNIL LHG+AMTT+AAR TA+KLLVV+SNGQ S GE
Sbjct: 732 VAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLVVISNGQRSFGE 791

Query: 794 VFLDDGEVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTFIGFE 853
           VFLDDGEV EMG EGGNWS+VRF SE VGSKL++KS+V+NGGFALSQKLIIDKVTF+GFE
Sbjct: 792 VFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFE 851

Query: 854 RHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVDISALSIPIWEEFKLELT 904
           R +KM G GL ISKG +LNGNS+I+KTY+Y  KF+NV+IS LSIPIWEEF LE+T
Sbjct: 852 RPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEEFILEMT 906

BLAST of MS013380 vs. ExPASy TrEMBL
Match: A0A1S3C8V0 (alpha-glucosidase OS=Cucumis melo OX=3656 GN=LOC103498289 PE=3 SV=1)

HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 735/895 (82.12%), Postives = 805/895 (89.94%), Query Frame = 0

Query: 14  RSLFGFSLFIVCFFF--SLAEPQPS---PVVGYGYRIRSAHVDPAGKSLTAGLALIRSSS 73
           R+ F  SL I+  F   S   P P+   P VG+GYRIRS+H+DPAGKSLTA L LIR+S 
Sbjct: 12  RTPFAHSLLILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPAGKSLTADLQLIRTSQ 71

Query: 74  VYGSDLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSPPESHVDSPA 133
           VYG DL TLTLQA+ E+KDRLR+RITDS RERWEIPD+IIPR SNS  RS PE+HV SP 
Sbjct: 72  VYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSNSLIRSLPENHVASPK 131

Query: 134 TASISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSS 193
            + ISDPASDL F+L +T PFGFSVLRRSSGDVLFDT+PD S+  TF+VFKDQYIQLSS 
Sbjct: 132 PSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSETFIVFKDQYIQLSSL 191

Query: 194 LPKDRSSLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRSPSR 253
           LPKDRSS+FGIGEQTR+SFKLV +K+KTLTLWNADIG   +DVNLYG+HPFYIDVRS SR
Sbjct: 192 LPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDVRSRSR 251

Query: 254 DGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIG 313
           DGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGG+IDLYFFAGPSP+SVIDQYTELIG
Sbjct: 252 DGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIG 311

Query: 314 RPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTFDPI 373
           RPAPVPYWSFG+HQCRYGYKNVS+VE+VVARYAK  IPLEVMWTDIDYMDGYKDFTFDPI
Sbjct: 312 RPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEVMWTDIDYMDGYKDFTFDPI 371

Query: 374 NFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYSGEVW 433
           NFP+EKMKIFVDNLH+NGQKYV ILDPGIS N TYG +IRGTKADIF+K++GVPY G+VW
Sbjct: 372 NFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRGTKADIFMKHDGVPYLGDVW 431

Query: 434 SGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDNPPY 493
            GPVYFPDF+HP+SE FWG EI++FRDIVPFDGLWIDMNEISN ITSSTSPLSNLDNPPY
Sbjct: 432 PGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNLDNPPY 491

Query: 494 KINNAAVLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVVSR 553
            INNA V RP+NN TVPAS LHFGNLTEYNTHNLYG LESRATHASLVKVTGKRPFV+SR
Sbjct: 492 MINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTGKRPFVLSR 551

Query: 554 STFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEELCRR 613
           STF GSGKYTAHWTGD  ATWND+GYTIPSILNFGLFGIPMVGSDICGF+GDTTEELCRR
Sbjct: 552 STFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRR 611

Query: 614 WIQLGAFYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHRKGT 673
           WIQLGAFYPFAR HSDK  IR+ELYLWDSVAA+ARKVLALRY+LLPYFYTLMYEAH+KGT
Sbjct: 612 WIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGT 671

Query: 674 PIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNYSES 733
           PIARPLFFSFPQDIKTYEI SQFLLG+GVL+SPVLKEGA SVDAYFP GNWFSLFNYSE 
Sbjct: 672 PIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEF 731

Query: 734 VSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSSDGE 793
           V+V SGQQ+ LDAPADHINVHVREGNIL LHG+AMTT+AAR TA+KLLVV+SNGQ S GE
Sbjct: 732 VAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLVVISNGQRSFGE 791

Query: 794 VFLDDGEVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTFIGFE 853
           VFLDDGEV EMG EGGNWS+VRF SE VGSKL++KS+V+NGGFALSQKLIIDKVTF+GFE
Sbjct: 792 VFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFE 851

Query: 854 RHEKMGGFGLKISKGANLNGNSAIKKTYEYSGKFVNVDISALSIPIWEEFKLELT 904
           R +KM G GL ISKG +LNGNS+I+KTY+Y  KF+NV+IS LSIPIWEEF LE+T
Sbjct: 852 RPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEEFILEMT 906

BLAST of MS013380 vs. TAIR 10
Match: AT5G11720.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 561/891 (62.96%), Postives = 688/891 (77.22%), Query Frame = 0

Query: 23  IVCFFFSL------AEPQPSPVVGYGYRIRSAHVDPAGKSLTAGLALIRSSSVYGSDLHT 82
           +V  FFSL       E + S VVGYGY +RS  VD   + LTA L LI+ SSVY  D+ +
Sbjct: 13  VVVVFFSLRSSQVVLEEEESTVVGYGYVVRSVGVDSNRQVLTAKLDLIKPSSVYAPDIKS 72

Query: 83  LTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFR--SPPESHVDSPATASISD 142
           L L  SLET +RLRIRITDS+++RWEIP+ +IPR  N   R  S  E   +SP    ++D
Sbjct: 73  LNLHVSLETSERLRIRITDSSQQRWEIPETVIPRAGNHSPRRFSTEEDGGNSPENNFLAD 132

Query: 143 PASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRS 202
           P+SDL F+L NTTPFGFSV RRSSGD+LFDT+PD SD  T+ +FKDQ++QLSS+LP++RS
Sbjct: 133 PSSDLVFTLHNTTPFGFSVSRRSSGDILFDTSPDSSDSNTYFIFKDQFLQLSSALPENRS 192

Query: 203 SLFGIGEQTRESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRSPSRDGKVVA 262
           +L+GIGE T+ SF+L+    +T+TLWNADIG    DVNLYGSHPFY+DVR     G   A
Sbjct: 193 NLYGIGEHTKRSFRLI--PGETMTLWNADIGSENPDVNLYGSHPFYMDVRGSK--GNEEA 252

Query: 263 GTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVP 322
           GTTHGVLLLNSNGMD+ Y G RITY VIGGVIDLY FAGPSP  V++QYTELIGRPAP+P
Sbjct: 253 GTTHGVLLLNSNGMDVKYEGHRITYNVIGGVIDLYVFAGPSPEMVMNQYTELIGRPAPMP 312

Query: 323 YWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTFDPINFPAEK 382
           YWSFG+HQCRYGYKNVSD+E VV  YAK GIPLEVMWTDIDYMDGYKDFT DP+NFP +K
Sbjct: 313 YWSFGFHQCRYGYKNVSDLEYVVDGYAKAGIPLEVMWTDIDYMDGYKDFTLDPVNFPEDK 372

Query: 383 MKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYSGEVWSGPVYF 442
           M+ FVD LH+NGQKYV ILDPGI ++ +YGT+ RG +AD+FIK NG PY GEVW G VYF
Sbjct: 373 MQSFVDTLHKNGQKYVLILDPGIGVDSSYGTYNRGMEADVFIKRNGEPYLGEVWPGKVYF 432

Query: 443 PDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAA 502
           PDF++P +  FW  EIK+F++I+P DGLWIDMNE+SN ITS  S  S+LD+PPYKINN+ 
Sbjct: 433 PDFLNPAAATFWSNEIKMFQEILPLDGLWIDMNELSNFITSPLSSGSSLDDPPYKINNSG 492

Query: 503 VLRPINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVVSRSTFVGS 562
             RPINN TVPA+S+HFGN++EY+ HNLYGLLE++ATH ++V +TGKRPF++SRSTFV S
Sbjct: 493 DKRPINNKTVPATSIHFGNISEYDAHNLYGLLEAKATHQAVVDITGKRPFILSRSTFVSS 552

Query: 563 GKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEELCRRWIQLGA 622
           GKYTAHWTGDN A W DL Y+IP ILNFGLFGIPMVG+DICGF+ DTTEELCRRWIQLGA
Sbjct: 553 GKYTAHWTGDNAAKWEDLAYSIPGILNFGLFGIPMVGADICGFSHDTTEELCRRWIQLGA 612

Query: 623 FYPFARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHRKGTPIARPL 682
           FYPFAR HS     R+ELYLWDSVA++ARKVL LR RLLP+ YTLMYEAH  G PIARPL
Sbjct: 613 FYPFARDHSSLGTARQELYLWDSVASSARKVLGLRMRLLPHLYTLMYEAHVSGNPIARPL 672

Query: 683 FFSFPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNYSESVSVKSG 742
           FFSFPQD KTYEI SQFL+GK +++SP LK+GA++VDAYFP GNWF LFNYS +V   SG
Sbjct: 673 FFSFPQDTKTYEIDSQFLIGKSIMVSPALKQGAVAVDAYFPAGNWFDLFNYSFAVGGDSG 732

Query: 743 QQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSSDGEVFLDDG 802
           + V LD PADH+NVHVREG+I+ + G+A+TTR AR T ++LLVV S  ++  GE+FLDDG
Sbjct: 733 KHVRLDTPADHVNVHVREGSIVAMQGEALTTRDARKTPYQLLVVASRLENISGELFLDDG 792

Query: 803 EVVEMGEEGGN--WSLVRFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTFIGFERHEK 862
           E + MG  GGN  W+LV+F     G  ++++SEVVN  +A   K  I KVTF+GFE  E 
Sbjct: 793 ENLRMGAGGGNRDWTLVKFRCYVTGKSVVLRSEVVNPEYASKMKWSIGKVTFVGFENVEN 852

Query: 863 MGGFGLKISKGANLNGNSAIKKTYEYSG-KFVNVDISALSIPIWEEFKLEL 903
           +  + ++ S+       S IK   +    +F++V++S LS+ + ++F++ L
Sbjct: 853 VKTYEVRTSERLRSPRISLIKTVSDNDDPRFLSVEVSKLSLLVGKKFEMRL 899

BLAST of MS013380 vs. TAIR 10
Match: AT1G68560.1 (alpha-xylosidase 1 )

HSP 1 Score: 794.7 bits (2051), Expect = 8.0e-230
Identity = 412/930 (44.30%), Postives = 580/930 (62.37%), Query Frame = 0

Query: 8   MAKTATRSLFGFSL---FIVCFFFSLAEPQPSPVVGYGYRIRSAHVDPAGKSLTAGLALI 67
           MA +++   F  SL    I+CF    +  Q    +G GYR+ S    P G      L + 
Sbjct: 1   MASSSSSLAFSLSLLLALILCF----SPTQSYKTIGKGYRLVSIEESPDG-GFIGYLQVK 60

Query: 68  RSSSVYGSDLHTLTLQASLETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSPPESHV 127
           + + +YGSD+ TL L    ET  RLR+ ITD+ ++RWE+P N++PR    +         
Sbjct: 61  QKNKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPREQPPQVGKVIGKSR 120

Query: 128 DSPATASISDPASDLAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQ 187
            SP T       S+L FS   T PF F+V RRS+ + LF+T        + LVFKDQY++
Sbjct: 121 KSPITVQ-EISGSELIFSY-TTDPFTFAVKRRSNHETLFNTT-------SSLVFKDQYLE 180

Query: 188 LSSSLPKDRSSLFGIGEQTR-ESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDV 247
           +S+SLPK+ +SL+G+GE ++    KLV N  +  TL+  D+    ++ +LYGSHP Y+D+
Sbjct: 181 ISTSLPKE-ASLYGLGENSQANGIKLVPN--EPYTLYTEDVSAINLNTDLYGSHPMYMDL 240

Query: 248 RSPSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQY 307
           R+    GK  A   H VLLLNSNGMD+ Y GD +TYKVIGGV D YF AGPSP++V+DQY
Sbjct: 241 RNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIAGPSPLNVVDQY 300

Query: 308 TELIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDF 367
           T+LIGRPAP+PYWS G+HQCR+GY N+S VE+VV  Y K  IPL+V+W D D+MDG+KDF
Sbjct: 301 TQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDVIWNDDDHMDGHKDF 360

Query: 368 TFDPINFPAEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPY 427
           T +P+ +P  K+  F+D +H+ G KY+ I DPGI +N +YGTF R   AD+FIKY G P+
Sbjct: 361 TLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRAMAADVFIKYEGKPF 420

Query: 428 SGEVWSGPVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISN------------ 487
             +VW GPVYFPDF++P +  +WG EIK F D+VP DGLWIDMNE+SN            
Sbjct: 421 LAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEVSNFCSGLCTIPEGK 480

Query: 488 ------------AITSSTSPLSNLDNPPYKINNAAVLRPINNNTVPASSLHFGNLTEYNT 547
                        +       +  D+PPYKIN   V+ P+   T+  S+ H+  + EY+ 
Sbjct: 481 QCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIATSATHYNGVREYDA 540

Query: 548 HNLYGLLESRATHASLVKVTGKRPFVVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSI 607
           H++YG  E+ ATH  L+ V GKRPF++SRSTFVGSG+Y AHWTGDN  TW  L  +I ++
Sbjct: 541 HSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVSISTM 600

Query: 608 LNFGLFGIPMVGSDICGFAGDTTEELCRRWIQLGAFYPFARGHSDKFYIRRELYLWDSVA 667
           LNFG+FG+PMVGSDICGF    TEELC RWI++GAFYPF+R H++ +  R+ELY WD+VA
Sbjct: 601 LNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQWDTVA 660

Query: 668 ATARKVLALRYRLLPYFYTLMYEAHRKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLI 727
            +AR  L +RY++LP+ YTL YEAH  G PIARPLFFSFP+  + Y  S QFLLG   +I
Sbjct: 661 DSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTECYGNSRQFLLGSSFMI 720

Query: 728 SPVLKEGALSVDAYFPEGNWFSLFNYSESVSVKSGQQVTLDAPADHINVHVREGNILVLH 787
           SPVL++G   V+A FP G+W+ +F+ +++V  K+G++VTL AP + +NVH+ +  IL   
Sbjct: 721 SPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNFVNVHLYQNTILPTQ 780

Query: 788 GDAMTTRAARGTAFKLLVVVSNGQS---SDGEVFLDDGEVVEMGEEGGNWSLVRFHSEAV 847
              + ++ AR T F L++    G S   + G+++LD+ E+ EM    G  + V F++   
Sbjct: 781 QGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMKLGNGQSTYVDFYASVG 840

Query: 848 GSKLLIKSEVVNGGFALSQKLIIDKVTFIGFERHEKMGGFGLKISKGANLNGNSAIKKTY 901
              + + S+V  G FALS+  +I+KV+ +G     ++    +  S        S+ + TY
Sbjct: 841 NGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQINGSPMTKKIEVSSKEHTY 900

BLAST of MS013380 vs. TAIR 10
Match: AT3G45940.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 768.1 bits (1982), Expect = 8.0e-222
Identity = 401/864 (46.41%), Postives = 549/864 (63.54%), Query Frame = 0

Query: 23  IVCFFFSLAEPQPSPVVGYGYRIRSAHVDPAGKSLTAGLALIRSSSVYGSDLHTLTLQAS 82
           +V      +  Q S  +G GYR+ S    P   S    L + +S+ +YGSD+  L L  +
Sbjct: 8   LVAIILCFSSLQCSNAIGKGYRLISMEKSPDDGSFIGYLQVKQSNKIYGSDITILRLFIN 67

Query: 83  LETKDRLRIRITDSARERWEIPDNIIPRRSNSRFRSPPE---SHVDSPATA-SISDPASD 142
             T  RLR+ ITD+ ++RWE+P N++ R        PP        SP T   IS P   
Sbjct: 68  YRTDHRLRVHITDAKKQRWEVPYNLLRR------EQPPNVIGKSRKSPVTVQEISGPELI 127

Query: 143 LAFSLRNTTPFGFSVLRRSSGDVLFDTAPDLSDPGTFLVFKDQYIQLSSSLPKDRSSLFG 202
           L F++    PF F+V RRS+G+ +F+T+      G  +VFKDQY+++S+SLPKD +SL+G
Sbjct: 128 LIFTV---DPFSFAVRRRSNGETIFNTSSSDESFGE-MVFKDQYLEISTSLPKD-ASLYG 187

Query: 203 IGEQTR-ESFKLVSNKSKTLTLWNADIGRAIVDVNLYGSHPFYIDVRSPSRDGKVVAGTT 262
            GE ++    KLV N  +  TL+  D+    ++ +LYGSHP Y+D+R+ S  GK  A   
Sbjct: 188 FGENSQANGIKLVPN--EPYTLFTEDVSAFNLNTDLYGSHPVYMDLRNVS--GKAYA--- 247

Query: 263 HGVLLLNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPAPVPYWS 322
           H VLLLNS+GMD+ Y GD +TYKVIGGV D YFFAGPSP++V+DQYT LIGRPAP+PYWS
Sbjct: 248 HSVLLLNSHGMDVFYRGDSLTYKVIGGVFDFYFFAGPSPLNVVDQYTSLIGRPAPMPYWS 307

Query: 323 FGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTFDPINFPAEKMKI 382
            G+HQCR+GY+NVS V++VV  Y K  IPL+V+W D DYMDGYKDFT D +NFP  K+  
Sbjct: 308 LGFHQCRWGYRNVSVVKDVVDNYQKAKIPLDVIWNDADYMDGYKDFTLDLVNFPHAKLLS 367

Query: 383 FVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFIKYNGVPYSGEVWSGPVYFPDF 442
           F+D +H+ G KYV I DPGI +N +YG + RG  +D+FIKY G P+  +VW GPVYFPDF
Sbjct: 368 FLDRIHKMGMKYVVIKDPGIGVNASYGVYQRGMASDVFIKYEGKPFLAQVWPGPVYFPDF 427

Query: 443 VHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLR 502
           ++P +  +WG EI+ F ++VP DGLWIDMNEI NA     S                   
Sbjct: 428 LNPKTVSWWGDEIRRFHELVPIDGLWIDMNEI-NATGHKAS------------------- 487

Query: 503 PINNNTVPASSLHFGNLTEYNTHNLYGLLESRATHASLVKVTGKRPFVVSRSTFVGSGKY 562
            +   T+P S+ H+  + EY+ H++YG  E+ ATH +L+ V GKRPF++SRSTFVGSG+Y
Sbjct: 488 -LGFKTIPTSAYHYNGVREYDAHSIYGFSEAIATHKALLAVQGKRPFILSRSTFVGSGQY 547

Query: 563 TAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTEELCRRWIQLGAFYP 622
            AHWTGDN  TW  L  +I ++LNFG+FG+PMVGSDICGF   T EELC RWI++GAFYP
Sbjct: 548 AAHWTGDNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFFPPTPEELCNRWIEVGAFYP 607

Query: 623 FARGHSDKFYIRRELYLWDSVAATARKVLALRYRLLPYFYTLMYEAHRKGTPIARPLFFS 682
           F+R H+D +  R+ELY W +VA +AR  L +RY+LLP+ YTL YEAH  G PIARPLFFS
Sbjct: 608 FSRDHADYYAPRKELYQWGTVAESARNALGMRYKLLPFLYTLNYEAHMSGAPIARPLFFS 667

Query: 683 FPQDIKTYEISSQFLLGKGVLISPVLKEGALSVDAYFPEGNWFSLFNYSESVSVKSGQQV 742
           FP+  + Y +S QFLLG  ++ISPVL++G   V+A FP G+W+ +F+ ++ V  K+G+  
Sbjct: 668 FPEFTECYGLSKQFLLGSSLMISPVLEQGKTQVEALFPPGSWYHMFDMTQVVVSKNGRLF 727

Query: 743 TLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKLLVVVSNGQSSDGEVFLDDGEVV 802
           TL AP + +NVH+ +  IL +         A           S G +S G++FLDD E+ 
Sbjct: 728 TLPAPFNVVNVHLYQNAILPMQQVVAFPAGA-----------SEGYAS-GKLFLDDDELP 787

Query: 803 EMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNGGFALSQKLIIDKVTFIGFERHEKMGGFG 862
           EM    G  + + F++      + I S+V  G FALSQ L+I+KV  +G +   K+    
Sbjct: 788 EMKLGNGKSTYIDFYASVGNESVKIWSQVKEGQFALSQGLVIEKVIVLGLKGTWKVSEI- 814

Query: 863 LKISKGANLNGNSAIKKTYEYSGK 882
             +  G++++  +   KT E S K
Sbjct: 848 --LLNGSSISNET---KTIEVSSK 814

BLAST of MS013380 vs. TAIR 10
Match: AT5G63840.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 320.1 bits (819), Expect = 5.8e-87
Identity = 225/691 (32.56%), Postives = 332/691 (48.05%), Query Frame = 0

Query: 192 DRSSLFGIGEQTRESFKLVSNK------SKTLTLWNADIGRAIVD--VNLYGSHPFYIDV 251
           D S ++GI E    SF L   K      S+   L+N D+     +    LYGS PF +  
Sbjct: 222 DSSFVYGIPEHA-TSFALKPTKGPGVEESEPYRLFNLDVFEYDHESPFGLYGSIPFMVS- 281

Query: 252 RSPSRDGKVVAGTTHGVLLLNS---------------NGMDIIYSGDRIT--YKVIGGVI 311
                 GK  +G T G   LN+               +G+ +  S  RI   +    G++
Sbjct: 282 -----HGK--SGKTSGFFWLNAAEMQIDVLANGWDAESGISLPSSHSRIDTFWMSEAGIV 341

Query: 312 DLYFFAGPSPVSVIDQYTELIGRPAPVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIP 371
           D +FF GP P  V+ QY  + G  A    ++ GYHQCR+ YK+  DV  V +++ +  IP
Sbjct: 342 DTFFFVGPEPKDVVKQYASVTGTSAMPQLFATGYHQCRWNYKDEEDVAQVDSKFDEHDIP 401

Query: 372 LEVMWTDIDYMDGYKDFTFDPINFP-AEKMKIFVDNLHQNGQKYVAILDPGISINKTYGT 431
            +V+W DI++ DG + FT+D + FP  E+M+     L   G+K V I+DP I  + +Y  
Sbjct: 402 YDVLWLDIEHTDGKRYFTWDSVLFPHPEEMQ---KKLAAKGRKMVTIVDPHIKRDDSYFL 461

Query: 432 FIRGTKADIFIK-YNGVPYSGEVWSGPVYFPDFVHPNSEIFWGGEI--KLFRDIVPFDGL 491
               T+   ++K  +G  + G  W G   + D + P    +WGG    K +    P    
Sbjct: 462 HKEATQMGYYVKDSSGKDFDGWCWPGSSSYIDMLSPEIRKWWGGRFSYKNYVGSTPSLYT 521

Query: 492 WIDMNEISNAITSSTSPLSNLDNPPYKINNAAVLRPINNNTVPASSLHFGNLTEYNTHNL 551
           W DMNE                  P   N   V       T+P  +LH G +     HN 
Sbjct: 522 WNDMNE------------------PSVFNGPEV-------TMPRDALHVGGVEHREVHNA 581

Query: 552 YGLLESRATHASLV-KVTGK-RPFVVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSIL 611
           YG     AT   LV +  GK RPFV+SR+ F G+ +Y A WTGDN A W  L  +IP IL
Sbjct: 582 YGYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSIPMIL 641

Query: 612 NFGLFGIPMVGSDICGFAGDTTEELCRRWIQLGAFYPFARGHSDKFYIRRELYLW-DSVA 671
             GL GI   G+DI GF G+   EL  RW Q+GA+YPF RGH+     RRE +L+ +   
Sbjct: 642 TLGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGERNT 701

Query: 672 ATARKVLALRYRLLPYFYTLMYEAHRKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLI 731
              R  +  RY LLPYFYTL  EA+  G P+ RPL+  FPQD  T+     F++G G+L+
Sbjct: 702 ELMRDAIHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEATFSNDEAFMVGSGLLV 761

Query: 732 SPVLKEGALSVDAYFP-EGNWFSLFNYSESVSVKSGQQVTLDAPADHINVHVREGNILVL 791
             V  +G      Y P + +W+ L N    V    G+   +DAP + I    + G I + 
Sbjct: 762 QGVYTKGTTQASVYLPGKESWYDLRNGKTYV---GGKTHKMDAPEESIPAFQKAGTI-IP 821

Query: 792 HGDAMTTRAAR--GTAFKLLVVVSNGQSSDGEVFLDDGEVVEMGEEGGNWSLVRFHSEAV 847
             D     +++     + L+V +++ Q ++GE+++DDG+  E     G++     H   V
Sbjct: 822 RKDRFRRSSSQMDNDPYTLVVALNSSQEAEGELYIDDGKSFEF--RRGSY----IHRRFV 865

BLAST of MS013380 vs. TAIR 10
Match: AT3G23640.1 (heteroglycan glucosidase 1 )

HSP 1 Score: 310.5 bits (794), Expect = 4.6e-84
Identity = 206/654 (31.50%), Postives = 321/654 (49.08%), Query Frame = 0

Query: 194 SSLFGIGEQTRESFKLVSNKSKTLTLWNADI-GRAIVDVNLYGSHPFYIDVRSPSRDGKV 253
           +S +G GE + +    +    K +  WN D  G      +LY SHP+ + V        V
Sbjct: 86  TSFYGTGEVSGQ----LERTGKRVFTWNTDAWGYGSGTTSLYQSHPWVLVVLPTGETLGV 145

Query: 254 VAGTTHGVLL-LNSNGMDIIYSGDRITYKVIGGVIDLYFFAGPSPVSVIDQYTELIGRPA 313
           +A TT    + L   G+  I S    +Y +I       F    SP +V++  +  IG   
Sbjct: 146 LADTTRKCEIDLRKEGIIRIIS--PASYPII------TFGPFSSPTAVLESLSHAIGTVF 205

Query: 314 PVPYWSFGYHQCRYGYKNVSDVENVVARYAKVGIPLEVMWTDIDYMDGYKDFTFDPINFP 373
             P W+ GYHQCR+ Y +   V  +   +    IP +V+W DIDYMDG++ FTFD   FP
Sbjct: 206 MPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDKERFP 265

Query: 374 AEKMKIFVDNLHQNGQKYVAILDPGISINKTYGTFIRGTKADIFI-KYNGVPYSGEVWSG 433
                    +LH NG K + +LDPGI   + Y  +  G+K D++I + +G P++GEVW G
Sbjct: 266 --DPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTGEVWPG 325

Query: 434 PVYFPDFVHPNSEIFWGGEIKLFRDIVPFDGLWIDMNEISNAITSSTSPLSNLDNPPYKI 493
           P  FPD+ +  +  +W   +K F      DG+W DMNE                      
Sbjct: 326 PCVFPDYTNSKARSWWANLVKEFVS-NGVDGIWNDMNE---------------------- 385

Query: 494 NNAAVLRPINNNTVPASSLHFGN------LTEYNTHNLYGLLESRATHASL-VKVTGKRP 553
              AV + +   T+P +++H G+          + HN+YG+L +R+T+  + +    KRP
Sbjct: 386 --PAVFKVV-TKTMPENNIHHGDDELGGVQNHSHYHNVYGMLMARSTYEGMELADKNKRP 445

Query: 554 FVVSRSTFVGSGKYTAHWTGDNDATWNDLGYTIPSILNFGLFGIPMVGSDICGFAGDTTE 613
           FV++R+ F+GS +Y A WTGDN + W  L  +I  +L  GL G P+ G DI GFAG+ T 
Sbjct: 446 FVLTRAGFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATP 505

Query: 614 ELCRRWIQLGAFYPFARGHSDKFYIRRELYLW-DSVAATARKVLALRYRLLPYFYTLMYE 673
            L  RW+ +GA +PF RGHS+      E + + +      R  L  RY+LLP+FYTL Y 
Sbjct: 506 RLFGRWMGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYI 565

Query: 674 AHRKGTPIARPLFFSFPQDIKTYEISSQFLLGKGVLISPVL-KEGALSVDAYFPEGNWFS 733
           AH  G P+A P+FF+ P D +   + + FLLG  ++ +  L  +G+  +    P G W  
Sbjct: 566 AHTTGAPVAAPIFFADPIDSRLRAVENGFLLGPLLIYASTLSSQGSHELQHILPRGIWHR 625

Query: 734 LFNYSES------VSVKSGQQVTLDAPADHINVHVREGNILVLHGDAMTTRAARGTAFKL 793
            F++++S      + ++ G  ++L  P    ++HV E ++     D +T          L
Sbjct: 626 -FDFADSHPDLPTLYLQGGSIISLAPP----HLHVGEFSL----SDDLT----------L 678

Query: 794 LVVVSNGQSSDGEVFLDDGEVVEMGEEGGNWSLVRFHSEAVGSKLLIKSEVVNG 830
           LV +     + G +F DDG+    G   G + +  + +E   S + +K     G
Sbjct: 686 LVSLDENGKAKGLLFEDDGD--GYGYTKGRFLVTHYIAERDSSTVTVKVSKTEG 678