MS013240 (gene) Bitter gourd (TR) v1

Overview
NameMS013240
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Description(S)-2-hydroxy-acid oxidase
Locationscaffold459: 1478240 .. 1482259 (+)
RNA-Seq ExpressionMS013240
SyntenyMS013240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCATCTGAACCAGTGAATGTGAATGACTATAAAGAATTGGCTAGGCAAGCACTTCCAAAAATGTATTATGATTACTATGCGGGAGGAGCTGAGGACCAGCACACACTTAGAGAAAACATGCAAGCTTTCCATAGAATCATGTAATTTTGAAGCTAGCTTATGATTACTGTGAAAAGTGATGCTATATGTTATTCATGAAATTTTAATTGTTTTCAGTATCCGACCTCGAATTCTTGTCGATGTAAGCAAAATTGATATGTCAACAACCATATTGGGCTACCGCATATCTGCACCCATCATGGTTGCTCCAACTGGTGCACACAAATTGGCACACTGTGAAGGTTAAAATTTTTGGTGGATTGCTTAGTTGAGAAATGGATAAATGGTTATCATTCCACGTCTCCTGGTTGATATTTGTCTTCTTTCAGGAGAGCTAGCCACAGCAAGGGCAGCAGCAGCTGCAAAAACCATAATGGTACTAATGTGACTGCAAACTGGAAGCCATTTTACTCATTTGGTATTTGAACTTAGTTGGTTTTGTGTGTAGGTTCTATCATATGCCTCATCCTACTCAATAGAGGAAGTTGCCTCTAGTTGCAATGCTGTTCGTTTTTTTCAATTATATGTAAATCCCACTCCTTCGAACTTTTCCTGTAGCAGAAATCAATTGTTTGTTTTTTTTAATTGAACTTCTCATTCTGTAATCAGGTTTTCAGAAAGCGTGATATCTCAGCTTTGCTAGTACAGAGAGCTGAGAGATCTGGATATAAGGCAATTGTCCTGACTGCTGATACTCCCCGGCTAGGTAGAAGGGAGGCTGACATAAAGAACAAGTAGCAATGCTTAACATCTTAGTTTCTTTCCTCTCTCATGCATTGCATTCCTTTTGCTGGGCATAAAGTGCTTCATGGAATTGGGATAAGATACATCGTCGTATCATAACTCTTTAAGTCGTGGGATAGGATGTTTTAAGTCATCAAAATTTTAAACAGTGCCTTGGTTTTGTTTCATCTGGAATGGGACTTTTATGAACTTGCAGGATGATTACACCTCCACTGAAGAACCTTGAAGGCCTGTTGTCTGTTGAAGTTGGCTCTGTGAGTAATATTTAACTTCCCTATTTCGGAATCTTGGAGATGCCAGTCTTAATAGAATGATTTCCAATCTCTCTAATGCCCATCGCATGGTATAAGATTGATTGTGTGCCTGCCATTTAATCTGGATAAAGTAAATTTAACTCATGATCTCATATGCAGGATAAAGGTTCAATATTAGAGTCTTTTGTTAACGAGGCTTTTGATGCATCTCTTTGCTGGGAAGTACGTGCAATAAACAGTTCAGATATCTATGCATACTTGATTGTCTGCGCTTATGAAAATTTTACTTTGATGCATGATAAGTTTTGAAGGGCATCCCTTTTGTAACTCGATAACTTAACTATTTCTATGTTATATAAATGGCTTCTACAGCATACCAGAAGTTTTAGTAATATCATCTCTTAATGAATTTTTTTACTGCTTAAAAGTTGTCTTTCTTATGTTTGATTTTACTGGCTCAGTTATGATATTTACCGTCCACAGGATATAGGGTGGTTAAAATCAATCACTAGCTTGCCAATTCTGATAAAGGGGATTCTCACTCACGAAGATGGTAAGATTAGAATTATGTCTTAAAGATATTTGAATTGAAATGAACCTTCTATTATCTCTATCAGATTAATGTTCTGAAATAACATATTTTACATTGTATTCAAATACTGCAAGTACGAACTCATTTTATCTGTCAATTAAGCTTCTGATATGATGGCTGCAGGAAAATGCATGATAATGTGTCTGGTTACCCTTTTTTTTTTGGCTTGATTAGTCCCTTGTTCTGGTTTGTGAGTTATGTTAGTGTCCATGGATAGCCCATTTAATTAGTTAACATGGTTGATGAATGGCAGCAATTAAGGCTGTGGAAGTAGGCGTTGATGGAATTATTGTCTCCAATCATGGTGCCCGCCAACTAGATTTTTCTCCTGCTACTGTTTCCGTCCTTGAAGAGGTAAATCTCATTTTGCACTATTTTTTTATTACGCGAAGCTGTCGGTACGAGGAAATATAATTATTCTTGAATTTCTGCATAGATTAAATGTTCAGTACCTAACTCTTTTGAAACGTACCTTCTTAAGGGATTGTAAATGACTCCCAGTATCTGTATTGCTCTTGTTTCTCTTTAATTTATAGCTTTTAGACAGATATCACTGCATCCAACTGTTCCTTCACAGCTTACTTTGCTCGTGTTGCTACATTCTCTCCTTTACTTGCACATTCTTTCAAAAACTTTGGTGAATTTACACAAGTTTGTCTTGTATGATGCATTTTCCATATTACTTGATATAGGAAAGCAATATGTGAATGTGGCTCTTGTTAGGGCCATAGAATCAGAACCAAGCATACCAAGTGTAGTTGCCCTCAAGGGACAGCGTAGTTGGCAAGTGCTTGGGGTCTCTTGGTCATACTTACTGGCTTAGAGGTCTCAAATTCGAACCTTCTGGCGAGCTTAATATCAAAAACCTATGATGTCTCCTAGGTCTGGCCCTTGGGGCGGGCACGGGTGCCCCTAGGTATAAGGGAGCAAAGTTTCGACTATCGGTTACCAAGTAGTTGAAATGTTGAATTGCATCCAACATCTACAGTATTATGCTTACTTTTAACAGGAAGTTTGATGCAGGTTGTTCATGCTGTCAAGGGTAAAGTTCCTGTCCTCTTAGATGGAGGTGTGCGACGAGGAACAGATGTGTTTAAGGCGTTAGCTCTTGGTGCCCAAGCAGTCCTTGTAAGCTCTATACTCCCAACTCTATCCATACAAAACATTTGAAGTCCCATAATACATTTTCGTTTCAAGTTTCAAATGATTACTACTATTTACAGACAAAGGTCTCCAAATTTTATTCTGTGCTATTTGGTATTTAGTCGCCATGCTCTTCAAACTCACTCAATTGGCCTTTTGGGCCATGTGTGATAAAATCCTTGTTTCTTACTTTCAACTCTTCCTCTCAGAAGTATTTCCAAGAACACTGGGTTTTCTTTTTGTTTTTGTTTTTTTTTTTACTCAAACCCTCGTAATATAGAACTAAATTAAATAGAATACATAGTAACTATCAAAAAATTAAATACAATTATGGTATAAATTAGGTTTAAATACTATTTTGATCCTTGTATTTTTTGCTTTGGTCCATGTTGGTTCTTGTAATTTCAAAACTTCTAGTTTGGTCTTTGTACTTTCAACTTTTGTTCATTTTAGTCTCTGTAAACGTCTCTTTTAGCTACTATACTTTTAAAAAGGGACCATTTTGGTCCCTTTACTTTCATTGTTATCTCATTTATTTTTACCATAATTTTAACAGGACACTTCACTCACTAAATTTCTTGAAATATAGCCATAAATTTTATTAAAGAGTTTCCATTGTGTTAAAATTTTGCATATCAGGACCAAATTGGACAGTTATTAAAAGTACTGGACCAAAACGTATCATTTAATAGTACAAGACTAAAATAAACAAAAGAAGAAAATACAGGGACTAAAAAGAATGTTCTGAAAGTAAGTAAAGATGACTAAAATGAACCAAAGTCAAAAGTACATAGAATAATTCCAGTTGGTTTTCCATATACAAAAACACGAGCAATTACGAAACCGATATTTCTTCCCTTCCAAATTTTATGAAATGAAAGCCAAAAAAAAAATAGGTAGCCGAACTCAATTCCTTCAAGTAAATCCAGAAGAAGAAGCATTGTGAAATGAAGTCTTAGTTAACTTGTTCATCTAACTTGTGACTGGAATGATTGGCTGATTCAGATAGGGAGGCCAGTTATATATGGGCTTGCAGCAAAGGGAGAAGAGGGAGTAAGAGCAGTGCTGGAAATGCTGAAGAATGAGTTAGAGATCACCATGGCTCTTTCTGGTTGCTCGTCTATCAAGGACATTACTAGAAGCCTTGTAAGGACACACTATGATCATAACTTGCAATCAATGCTT

mRNA sequence

ATGTCATCTGAACCAGTGAATGTGAATGACTATAAAGAATTGGCTAGGCAAGCACTTCCAAAAATGTATTATGATTACTATGCGGGAGGAGCTGAGGACCAGCACACACTTAGAGAAAACATGCAAGCTTTCCATAGAATCATTATCCGACCTCGAATTCTTGTCGATGTAAGCAAAATTGATATGTCAACAACCATATTGGGCTACCGCATATCTGCACCCATCATGGTTGCTCCAACTGGTGCACACAAATTGGCACACTGTGAAGGAGAGCTAGCCACAGCAAGGGCAGCAGCAGCTGCAAAAACCATAATGGTTCTATCATATGCCTCATCCTACTCAATAGAGGAAGTTGCCTCTAGTTGCAATGCTGTTCGTTTTTTTCAATTATATGTTTTCAGAAAGCGTGATATCTCAGCTTTGCTAGTACAGAGAGCTGAGAGATCTGGATATAAGGCAATTGTCCTGACTGCTGATACTCCCCGGCTAGGTAGAAGGGAGGCTGACATAAAGAACAAGATGATTACACCTCCACTGAAGAACCTTGAAGGCCTGTTGTCTGTTGAAGTTGGCTCTGATAAAGGTTCAATATTAGAGTCTTTTGTTAACGAGGCTTTTGATGCATCTCTTTGCTGGGAAGATATAGGGTGGTTAAAATCAATCACTAGCTTGCCAATTCTGATAAAGGGGATTCTCACTCACGAAGATGCAATTAAGGCTGTGGAAGTAGGCGTTGATGGAATTATTGTCTCCAATCATGGTGCCCGCCAACTAGATTTTTCTCCTGCTACTGTTTCCGTCCTTGAAGAGGTTGTTCATGCTGTCAAGGGTAAAGTTCCTGTCCTCTTAGATGGAGGTGTGCGACGAGGAACAGATGTGTTTAAGGCGTTAGCTCTTGGTGCCCAAGCAGTCCTTATAGGGAGGCCAGTTATATATGGGCTTGCAGCAAAGGGAGAAGAGGGAGTAAGAGCAGTGCTGGAAATGCTGAAGAATGAGTTAGAGATCACCATGGCTCTTTCTGGTTGCTCGTCTATCAAGGACATTACTAGAAGCCTTGTAAGGACACACTATGATCATAACTTGCAATCAATGCTT

Coding sequence (CDS)

ATGTCATCTGAACCAGTGAATGTGAATGACTATAAAGAATTGGCTAGGCAAGCACTTCCAAAAATGTATTATGATTACTATGCGGGAGGAGCTGAGGACCAGCACACACTTAGAGAAAACATGCAAGCTTTCCATAGAATCATTATCCGACCTCGAATTCTTGTCGATGTAAGCAAAATTGATATGTCAACAACCATATTGGGCTACCGCATATCTGCACCCATCATGGTTGCTCCAACTGGTGCACACAAATTGGCACACTGTGAAGGAGAGCTAGCCACAGCAAGGGCAGCAGCAGCTGCAAAAACCATAATGGTTCTATCATATGCCTCATCCTACTCAATAGAGGAAGTTGCCTCTAGTTGCAATGCTGTTCGTTTTTTTCAATTATATGTTTTCAGAAAGCGTGATATCTCAGCTTTGCTAGTACAGAGAGCTGAGAGATCTGGATATAAGGCAATTGTCCTGACTGCTGATACTCCCCGGCTAGGTAGAAGGGAGGCTGACATAAAGAACAAGATGATTACACCTCCACTGAAGAACCTTGAAGGCCTGTTGTCTGTTGAAGTTGGCTCTGATAAAGGTTCAATATTAGAGTCTTTTGTTAACGAGGCTTTTGATGCATCTCTTTGCTGGGAAGATATAGGGTGGTTAAAATCAATCACTAGCTTGCCAATTCTGATAAAGGGGATTCTCACTCACGAAGATGCAATTAAGGCTGTGGAAGTAGGCGTTGATGGAATTATTGTCTCCAATCATGGTGCCCGCCAACTAGATTTTTCTCCTGCTACTGTTTCCGTCCTTGAAGAGGTTGTTCATGCTGTCAAGGGTAAAGTTCCTGTCCTCTTAGATGGAGGTGTGCGACGAGGAACAGATGTGTTTAAGGCGTTAGCTCTTGGTGCCCAAGCAGTCCTTATAGGGAGGCCAGTTATATATGGGCTTGCAGCAAAGGGAGAAGAGGGAGTAAGAGCAGTGCTGGAAATGCTGAAGAATGAGTTAGAGATCACCATGGCTCTTTCTGGTTGCTCGTCTATCAAGGACATTACTAGAAGCCTTGTAAGGACACACTATGATCATAACTTGCAATCAATGCTT

Protein sequence

MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMSTTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQSML
Homology
BLAST of MS013240 vs. NCBI nr
Match: XP_022148211.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Momordica charantia])

HSP 1 Score: 697.6 bits (1799), Expect = 5.8e-197
Identity = 363/365 (99.45%), Postives = 363/365 (99.45%), Query Frame = 0

Query: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60
           MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI
Sbjct: 10  MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 69

Query: 61  DMSTTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120
           DMSTTILGY IS PIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS
Sbjct: 70  DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 129

Query: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180
           SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK
Sbjct: 130 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 189

Query: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240
           NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA
Sbjct: 190 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 249

Query: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300
           VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG
Sbjct: 250 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 309

Query: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360
           AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN
Sbjct: 310 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 369

Query: 361 LQSML 366
           LQSML
Sbjct: 370 LQSML 374

BLAST of MS013240 vs. NCBI nr
Match: XP_022148212.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Momordica charantia] >XP_022148213.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Momordica charantia] >XP_022148214.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Momordica charantia] >XP_022148215.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Momordica charantia])

HSP 1 Score: 697.6 bits (1799), Expect = 5.8e-197
Identity = 363/365 (99.45%), Postives = 363/365 (99.45%), Query Frame = 0

Query: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60
           MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI
Sbjct: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60

Query: 61  DMSTTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120
           DMSTTILGY IS PIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS
Sbjct: 61  DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120

Query: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180
           SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK
Sbjct: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180

Query: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240
           NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA
Sbjct: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240

Query: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300
           VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG
Sbjct: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300

Query: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360
           AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN
Sbjct: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360

Query: 361 LQSML 366
           LQSML
Sbjct: 361 LQSML 365

BLAST of MS013240 vs. NCBI nr
Match: XP_022148216.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X3 [Momordica charantia])

HSP 1 Score: 641.3 bits (1653), Expect = 4.9e-180
Identity = 342/365 (93.70%), Postives = 342/365 (93.70%), Query Frame = 0

Query: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60
           MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI
Sbjct: 10  MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 69

Query: 61  DMSTTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120
           DMSTTILGY IS PIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS
Sbjct: 70  DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 129

Query: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180
           SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK
Sbjct: 130 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 189

Query: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240
           NLEGLLSVEVGS                     DIGWLKSITSLPILIKGILTHEDAIKA
Sbjct: 190 NLEGLLSVEVGS---------------------DIGWLKSITSLPILIKGILTHEDAIKA 249

Query: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300
           VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG
Sbjct: 250 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 309

Query: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360
           AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN
Sbjct: 310 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 353

Query: 361 LQSML 366
           LQSML
Sbjct: 370 LQSML 353

BLAST of MS013240 vs. NCBI nr
Match: XP_038894778.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Benincasa hispida] >XP_038894784.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Benincasa hispida] >XP_038894792.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Benincasa hispida] >XP_038894801.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 634.8 bits (1636), Expect = 4.6e-178
Identity = 329/367 (89.65%), Postives = 352/367 (95.91%), Query Frame = 0

Query: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60
           MS+EPVNV+D+KELARQALPKMYYDYYAGGAEDQHTLR+NMQAF+RI+I+PRILVDVSKI
Sbjct: 1   MSTEPVNVDDFKELARQALPKMYYDYYAGGAEDQHTLRQNMQAFYRIMIQPRILVDVSKI 60

Query: 61  DMSTTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120
           DMSTTILGYRISAPIMVAPTGAHKLA+ EGELATARAAAAAKTIMVLSYASSYS+EEVA+
Sbjct: 61  DMSTTILGYRISAPIMVAPTGAHKLAYYEGELATARAAAAAKTIMVLSYASSYSMEEVAT 120

Query: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180
           SCNA+RFFQLYVF++R+IS LLVQRAER GYKAIVLTADTPRLGRREADIKNKMITPPLK
Sbjct: 121 SCNAIRFFQLYVFKRREISTLLVQRAERFGYKAIVLTADTPRLGRREADIKNKMITPPLK 180

Query: 181 NLEGLLSV--EVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAI 240
           NLEGLLSV  EV SDKGS+LESFVNEAFD+SLCW+DI WLKSITSLP+L+KGILTHEDAI
Sbjct: 181 NLEGLLSVKIEVDSDKGSLLESFVNEAFDSSLCWKDIAWLKSITSLPVLVKGILTHEDAI 240

Query: 241 KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA 300
           KAVEVGVDGIIVSNHGARQLDFSP T+SV+EEVVHAVKGKVPVLLDGGVRRGTDVFKALA
Sbjct: 241 KAVEVGVDGIIVSNHGARQLDFSPPTISVVEEVVHAVKGKVPVLLDGGVRRGTDVFKALA 300

Query: 301 LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 360
           LGAQAVL+GRPVIYGLAAKGEEGVR VLEMLKNELEI+MALSGC  IKDITRS VRTHYD
Sbjct: 301 LGAQAVLVGRPVIYGLAAKGEEGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTHYD 360

Query: 361 HNLQSML 366
             L SML
Sbjct: 361 -KLHSML 366

BLAST of MS013240 vs. NCBI nr
Match: XP_022148294.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Momordica charantia] >XP_022148295.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Momordica charantia] >XP_022148296.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Momordica charantia])

HSP 1 Score: 612.5 bits (1578), Expect = 2.4e-171
Identity = 317/365 (86.85%), Postives = 343/365 (93.97%), Query Frame = 0

Query: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60
           MSSEP+NVND+KELARQALPKMYYD+YAGGAEDQHTLREN+QAFH+I IRPR+LVDVSKI
Sbjct: 1   MSSEPMNVNDFKELARQALPKMYYDFYAGGAEDQHTLRENIQAFHKITIRPRVLVDVSKI 60

Query: 61  DMSTTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120
           DMSTTILGYRISAPIMVAPT AHKLA+ EGELATARAAAAAKTIMVLSY+++ SIEEVAS
Sbjct: 61  DMSTTILGYRISAPIMVAPTAAHKLAYHEGELATARAAAAAKTIMVLSYSATSSIEEVAS 120

Query: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180
           SC+AVRFFQLYVF++RDI+ LLV+RAERSGYKAIVLTADTPRLGRREADIKNKMI PP+K
Sbjct: 121 SCDAVRFFQLYVFKRRDITTLLVKRAERSGYKAIVLTADTPRLGRREADIKNKMIAPPVK 180

Query: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240
           NLEGL+SVE+ SD+GS LE+  N   DASL WEDIGWL+SITSLPILIKGILTHEDA +A
Sbjct: 181 NLEGLVSVEINSDQGSKLEAHANATLDASLRWEDIGWLRSITSLPILIKGILTHEDATRA 240

Query: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300
           VE GVDGIIVSNHGARQLDF+PATVSVLEEVVHAVKG+VPVL DGGVRRGTDVFKALALG
Sbjct: 241 VEAGVDGIIVSNHGARQLDFAPATVSVLEEVVHAVKGRVPVLFDGGVRRGTDVFKALALG 300

Query: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360
           AQAVLIGRPV++GLAAKGEEGVR VLEMLKNELEITMALSGC SIKDITRSLVRT YD  
Sbjct: 301 AQAVLIGRPVLFGLAAKGEEGVRTVLEMLKNELEITMALSGCPSIKDITRSLVRTQYD-K 360

Query: 361 LQSML 366
           LQSML
Sbjct: 361 LQSML 364

BLAST of MS013240 vs. ExPASy Swiss-Prot
Match: Q9LJH5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=GLO4 PE=2 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 7.9e-149
Identity = 260/360 (72.22%), Postives = 321/360 (89.17%), Query Frame = 0

Query: 6   VNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMSTT 65
           VNV++++ELA+QALPKMYYD+Y GGAEDQHTL EN+QAF RI+ RPR+LVDVS IDMST+
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSNIDMSTS 64

Query: 66  ILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNAV 125
           +LGY ISAPIM+APT  HKLAH +GE+ATA+AAAA  TIM++S+ S+ +IEEVASSCNAV
Sbjct: 65  MLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIVSFMSTCTIEEVASSCNAV 124

Query: 126 RFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEGL 185
           RF Q+YV+++RD++A +V+RAE++G+KAIVLT D PRLGRREADIKNKMI+P LKN EGL
Sbjct: 125 RFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 186 LSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEVGV 245
           +S EV  ++GS +E+F + AFDASL W+DI WL+SIT LPIL+KG+LT EDA+KAVE GV
Sbjct: 185 VSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKLPILVKGLLTREDALKAVEAGV 244

Query: 246 DGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVL 305
           DGI+VSNHGARQLD+SPAT++VLEEVVHAVKG++PVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIVVSNHGARQLDYSPATITVLEEVVHAVKGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 306 IGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQSML 365
           IGRP++YGLAAKGE+GV+ V++MLKNE EITMALSGC +I D+TR+ VRT  +  ++SML
Sbjct: 305 IGRPIVYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDVTRNHVRTE-NERIKSML 363

BLAST of MS013240 vs. ExPASy Swiss-Prot
Match: Q24JJ8 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=GLO3 PE=2 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 2.3e-148
Identity = 265/360 (73.61%), Postives = 313/360 (86.94%), Query Frame = 0

Query: 6   VNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMSTT 65
           VNV++++ELA+QALPKMYYD+Y GGAEDQHTL EN+QAF RI+ RPR+LVDVSKIDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 66  ILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNAV 125
           ILGY ISAPIM+APTG HKLAH EGE ATA+AAAA  TIM++SY SS + EE+ASSCNAV
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 126 RFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEGL 185
           RF Q+YV+++RDI+A +V+RAE++G+KAIVLT D PRLGRREADIKNKMI+P LKN EGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 186 LSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEVGV 245
            S EV   KGS +++F + AFDAS  W+DI WL+SIT LPIL+KGILT EDA+KAVE GV
Sbjct: 185 FSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGV 244

Query: 246 DGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVL 305
           DGIIVSNHG RQLD+SPAT++VLEEVV  V+G++PVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 306 IGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQSML 365
           IGRP+IYGLAAKGE+GV+ V++MLKNE EITMALSGC +I DITR+ VRT  +  L SML
Sbjct: 305 IGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTE-NERLHSML 363

BLAST of MS013240 vs. ExPASy Swiss-Prot
Match: Q8H3I4 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=39947 GN=GLO4 PE=1 SV=2)

HSP 1 Score: 467.2 bits (1201), Expect = 1.7e-130
Identity = 238/362 (65.75%), Postives = 295/362 (81.49%), Query Frame = 0

Query: 5   PVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMST 64
           PVNV +Y+ELA++ALPKM YDY  GGAED+HTLREN+ A+ RII+RPR+LVDVSKIDMST
Sbjct: 6   PVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMST 65

Query: 65  TILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNA 124
           T+LGY + +PI+VAPTG HKLAH EGE ATARAAA+   IMVLS++SS  IE+VASSCNA
Sbjct: 66  TLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNA 125

Query: 125 VRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEG 184
           +RF+QLYV++ R++SA LV+RAE  G+KA++LT DTP LGRREADI+NKM+ P   NLEG
Sbjct: 126 IRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEG 185

Query: 185 LLSV-EVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEV 244
           L++  +  +  GS LE F     D SL W+DI WLKSITS+PI +KGI+T EDA +AVE 
Sbjct: 186 LMTTDDHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEA 245

Query: 245 GVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQA 304
           GV G+IVSNHGARQLD++PAT++ LEEVV AV G VPVL+DGG+RRGTDVFKALALGA+A
Sbjct: 246 GVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARA 305

Query: 305 VLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQS 364
           V++GRPV +GLAA+GE G R V+EML  ELE+ MAL GC S+ +ITRS V T  D  ++S
Sbjct: 306 VMVGRPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHVMTEGD-RIRS 365

Query: 365 ML 366
           +L
Sbjct: 366 LL 366

BLAST of MS013240 vs. ExPASy Swiss-Prot
Match: B8B8K5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=39946 GN=GLO4 PE=3 SV=2)

HSP 1 Score: 461.8 bits (1187), Expect = 7.0e-129
Identity = 236/362 (65.19%), Postives = 293/362 (80.94%), Query Frame = 0

Query: 5   PVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMST 64
           PVNV +Y+ELA++ALPKM YDY  GGAED+HTLREN+ A+ RII+RPR+LVDVSKIDMST
Sbjct: 6   PVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMST 65

Query: 65  TILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNA 124
           T+LGY + +PI+VAPTG HKLAH EGE ATARAAA+   IMVLS++SS  IE+VASSCNA
Sbjct: 66  TLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNA 125

Query: 125 VRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEG 184
           +RF+QLYV++ R++SA LV+RAE  G+KA++LT DTP LGRREADI+NKM+ P   NLEG
Sbjct: 126 IRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEG 185

Query: 185 LLSV-EVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEV 244
           L+++ +  +  GS LE F     D SL W+DI WLKSITS+PI +KGI+T EDA +AVE 
Sbjct: 186 LMTIDDHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEA 245

Query: 245 GVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQA 304
           GV G+IVSNHGARQLD++PAT++ LEEVV AV G VPVL+DGG+RRGTDVFKALALGA+A
Sbjct: 246 GVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARA 305

Query: 305 VLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQS 364
           V+   PV +GLAA+GE G R V+EML  ELE+ MAL GC S+ +ITRS V T  D  ++S
Sbjct: 306 VMXXXPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHVMTEGD-RIRS 365

Query: 365 ML 366
           +L
Sbjct: 366 LL 366

BLAST of MS013240 vs. ExPASy Swiss-Prot
Match: P05414 (Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 7.7e-120
Identity = 221/358 (61.73%), Postives = 279/358 (77.93%), Query Frame = 0

Query: 4   EPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMS 63
           E  NVN+Y+ +A+Q LPKM YDYYA GAEDQ TL EN  AF RI+ RPRIL+DV+ IDM+
Sbjct: 2   EITNVNEYEAIAKQKLPKMVYDYYASGAEDQWTLAENRNAFSRILFRPRILIDVTNIDMT 61

Query: 64  TTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCN 123
           TTILG++IS PIM+APT   K+AH EGE ATARAA+AA TIM LS  ++ S+EEVAS+  
Sbjct: 62  TTILGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 124 AVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP---LK 183
            +RFFQLYV++ R++ A LV+RAER+G+KAI LT DTPRLGRREADIKN+ + PP   LK
Sbjct: 122 GIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLK 181

Query: 184 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 243
           N EG+   ++     S L S+V    D SL W+D+ WL++ITSLPIL+KG++T EDA  A
Sbjct: 182 NFEGIDLGKMDKANDSGLSSYVAGQIDRSLSWKDVAWLQTITSLPILVKGVITAEDARLA 241

Query: 244 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 303
           V+ G  GIIVSNHGARQLD+ PAT+  LEEVV A +G++PV LDGGVRRGTDVFKALALG
Sbjct: 242 VQHGAAGIIVSNHGARQLDYVPATIMALEEVVKAAQGRIPVFLDGGVRRGTDVFKALALG 301

Query: 304 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 359
           A  V IGRPV++ LAA+GE GV+ VL+M+++E E+TMALSGC S+K+I+RS +   +D
Sbjct: 302 AAGVFIGRPVVFSLAAEGEAGVKKVLQMMRDEFELTMALSGCRSLKEISRSHIAADWD 359

BLAST of MS013240 vs. ExPASy TrEMBL
Match: A0A6J1D2A9 ((S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 2.8e-197
Identity = 363/365 (99.45%), Postives = 363/365 (99.45%), Query Frame = 0

Query: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60
           MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI
Sbjct: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60

Query: 61  DMSTTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120
           DMSTTILGY IS PIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS
Sbjct: 61  DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120

Query: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180
           SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK
Sbjct: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180

Query: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240
           NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA
Sbjct: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240

Query: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300
           VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG
Sbjct: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300

Query: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360
           AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN
Sbjct: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360

Query: 361 LQSML 366
           LQSML
Sbjct: 361 LQSML 365

BLAST of MS013240 vs. ExPASy TrEMBL
Match: A0A6J1D4P5 ((S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 2.8e-197
Identity = 363/365 (99.45%), Postives = 363/365 (99.45%), Query Frame = 0

Query: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60
           MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI
Sbjct: 10  MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 69

Query: 61  DMSTTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120
           DMSTTILGY IS PIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS
Sbjct: 70  DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 129

Query: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180
           SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK
Sbjct: 130 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 189

Query: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240
           NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA
Sbjct: 190 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 249

Query: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300
           VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG
Sbjct: 250 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 309

Query: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360
           AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN
Sbjct: 310 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 369

Query: 361 LQSML 366
           LQSML
Sbjct: 370 LQSML 374

BLAST of MS013240 vs. ExPASy TrEMBL
Match: A0A6J1D4Q2 ((S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.4e-180
Identity = 342/365 (93.70%), Postives = 342/365 (93.70%), Query Frame = 0

Query: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60
           MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI
Sbjct: 10  MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 69

Query: 61  DMSTTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120
           DMSTTILGY IS PIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS
Sbjct: 70  DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 129

Query: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180
           SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK
Sbjct: 130 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 189

Query: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240
           NLEGLLSVEVGS                     DIGWLKSITSLPILIKGILTHEDAIKA
Sbjct: 190 NLEGLLSVEVGS---------------------DIGWLKSITSLPILIKGILTHEDAIKA 249

Query: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300
           VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG
Sbjct: 250 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 309

Query: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360
           AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN
Sbjct: 310 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 353

Query: 361 LQSML 366
           LQSML
Sbjct: 370 LQSML 353

BLAST of MS013240 vs. ExPASy TrEMBL
Match: A0A6J1D4Y8 ((S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016982 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.2e-171
Identity = 317/365 (86.85%), Postives = 343/365 (93.97%), Query Frame = 0

Query: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60
           MSSEP+NVND+KELARQALPKMYYD+YAGGAEDQHTLREN+QAFH+I IRPR+LVDVSKI
Sbjct: 1   MSSEPMNVNDFKELARQALPKMYYDFYAGGAEDQHTLRENIQAFHKITIRPRVLVDVSKI 60

Query: 61  DMSTTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120
           DMSTTILGYRISAPIMVAPT AHKLA+ EGELATARAAAAAKTIMVLSY+++ SIEEVAS
Sbjct: 61  DMSTTILGYRISAPIMVAPTAAHKLAYHEGELATARAAAAAKTIMVLSYSATSSIEEVAS 120

Query: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180
           SC+AVRFFQLYVF++RDI+ LLV+RAERSGYKAIVLTADTPRLGRREADIKNKMI PP+K
Sbjct: 121 SCDAVRFFQLYVFKRRDITTLLVKRAERSGYKAIVLTADTPRLGRREADIKNKMIAPPVK 180

Query: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240
           NLEGL+SVE+ SD+GS LE+  N   DASL WEDIGWL+SITSLPILIKGILTHEDA +A
Sbjct: 181 NLEGLVSVEINSDQGSKLEAHANATLDASLRWEDIGWLRSITSLPILIKGILTHEDATRA 240

Query: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300
           VE GVDGIIVSNHGARQLDF+PATVSVLEEVVHAVKG+VPVL DGGVRRGTDVFKALALG
Sbjct: 241 VEAGVDGIIVSNHGARQLDFAPATVSVLEEVVHAVKGRVPVLFDGGVRRGTDVFKALALG 300

Query: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360
           AQAVLIGRPV++GLAAKGEEGVR VLEMLKNELEITMALSGC SIKDITRSLVRT YD  
Sbjct: 301 AQAVLIGRPVLFGLAAKGEEGVRTVLEMLKNELEITMALSGCPSIKDITRSLVRTQYD-K 360

Query: 361 LQSML 366
           LQSML
Sbjct: 361 LQSML 364

BLAST of MS013240 vs. ExPASy TrEMBL
Match: A0A1S3CCP5 ((S)-2-hydroxy-acid oxidase OS=Cucumis melo OX=3656 GN=LOC103499183 PE=3 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.2e-168
Identity = 308/365 (84.38%), Postives = 340/365 (93.15%), Query Frame = 0

Query: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60
           MSSEPVNVND+KELAR ALPKMYYD+Y+GGAED+HTLREN+QAF+RI IRPR+LVDVSK+
Sbjct: 1   MSSEPVNVNDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKV 60

Query: 61  DMSTTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120
           DMSTTILG+ ISAPI+VAPT AHKLA  EGELATARAAAA KTIMVLSY+S+ SIEEVAS
Sbjct: 61  DMSTTILGHHISAPILVAPTAAHKLAFHEGELATARAAAAVKTIMVLSYSSTCSIEEVAS 120

Query: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180
           SCN+VRFFQLY+F++RDISALLVQRAER GYKAI+LT DTPRLGRREADIKNKMI PP+K
Sbjct: 121 SCNSVRFFQLYIFKRRDISALLVQRAERCGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180

Query: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240
           +LEGL+S++V SD+GS LE++ NE  DASL WEDIGWL+SIT+LPILIKG+LTHEDA KA
Sbjct: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLSWEDIGWLRSITTLPILIKGVLTHEDATKA 240

Query: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300
           VE GVDGIIVSNHGARQLDF+PATVSVLEEVVHAVKGK+PVLLDGGVRRGTDVFKALALG
Sbjct: 241 VEAGVDGIIVSNHGARQLDFAPATVSVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300

Query: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360
           AQAVLIGRPVIYGLAAKGEEGVR V+EMLKNELE +MALSGC SIKDITRS VRTHYD N
Sbjct: 301 AQAVLIGRPVIYGLAAKGEEGVRTVMEMLKNELETSMALSGCPSIKDITRSHVRTHYD-N 360

Query: 361 LQSML 366
           L SML
Sbjct: 361 LPSML 364

BLAST of MS013240 vs. TAIR 10
Match: AT3G14130.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 528.1 bits (1359), Expect = 5.6e-150
Identity = 260/360 (72.22%), Postives = 321/360 (89.17%), Query Frame = 0

Query: 6   VNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMSTT 65
           VNV++++ELA+QALPKMYYD+Y GGAEDQHTL EN+QAF RI+ RPR+LVDVS IDMST+
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSNIDMSTS 64

Query: 66  ILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNAV 125
           +LGY ISAPIM+APT  HKLAH +GE+ATA+AAAA  TIM++S+ S+ +IEEVASSCNAV
Sbjct: 65  MLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIVSFMSTCTIEEVASSCNAV 124

Query: 126 RFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEGL 185
           RF Q+YV+++RD++A +V+RAE++G+KAIVLT D PRLGRREADIKNKMI+P LKN EGL
Sbjct: 125 RFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 186 LSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEVGV 245
           +S EV  ++GS +E+F + AFDASL W+DI WL+SIT LPIL+KG+LT EDA+KAVE GV
Sbjct: 185 VSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKLPILVKGLLTREDALKAVEAGV 244

Query: 246 DGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVL 305
           DGI+VSNHGARQLD+SPAT++VLEEVVHAVKG++PVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIVVSNHGARQLDYSPATITVLEEVVHAVKGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 306 IGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQSML 365
           IGRP++YGLAAKGE+GV+ V++MLKNE EITMALSGC +I D+TR+ VRT  +  ++SML
Sbjct: 305 IGRPIVYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDVTRNHVRTE-NERIKSML 363

BLAST of MS013240 vs. TAIR 10
Match: AT3G14150.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 526.6 bits (1355), Expect = 1.6e-149
Identity = 265/360 (73.61%), Postives = 313/360 (86.94%), Query Frame = 0

Query: 6   VNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMSTT 65
           VNV++++ELA+QALPKMYYD+Y GGAEDQHTL EN+QAF RI+ RPR+LVDVSKIDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 66  ILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNAV 125
           ILGY ISAPIM+APTG HKLAH EGE ATA+AAAA  TIM++SY SS + EE+ASSCNAV
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 126 RFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEGL 185
           RF Q+YV+++RDI+A +V+RAE++G+KAIVLT D PRLGRREADIKNKMI+P LKN EGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 186 LSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEVGV 245
            S EV   KGS +++F + AFDAS  W+DI WL+SIT LPIL+KGILT EDA+KAVE GV
Sbjct: 185 FSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGV 244

Query: 246 DGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVL 305
           DGIIVSNHG RQLD+SPAT++VLEEVV  V+G++PVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 306 IGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQSML 365
           IGRP+IYGLAAKGE+GV+ V++MLKNE EITMALSGC +I DITR+ VRT  +  L SML
Sbjct: 305 IGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTE-NERLHSML 363

BLAST of MS013240 vs. TAIR 10
Match: AT3G14150.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 526.6 bits (1355), Expect = 1.6e-149
Identity = 265/360 (73.61%), Postives = 313/360 (86.94%), Query Frame = 0

Query: 6   VNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMSTT 65
           VNV++++ELA+QALPKMYYD+Y GGAEDQHTL EN+QAF RI+ RPR+LVDVSKIDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 66  ILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNAV 125
           ILGY ISAPIM+APTG HKLAH EGE ATA+AAAA  TIM++SY SS + EE+ASSCNAV
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 126 RFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEGL 185
           RF Q+YV+++RDI+A +V+RAE++G+KAIVLT D PRLGRREADIKNKMI+P LKN EGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 186 LSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEVGV 245
            S EV   KGS +++F + AFDAS  W+DI WL+SIT LPIL+KGILT EDA+KAVE GV
Sbjct: 185 FSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGV 244

Query: 246 DGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVL 305
           DGIIVSNHG RQLD+SPAT++VLEEVV  V+G++PVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 306 IGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQSML 365
           IGRP+IYGLAAKGE+GV+ V++MLKNE EITMALSGC +I DITR+ VRT  +  L SML
Sbjct: 305 IGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTE-NERLHSML 363

BLAST of MS013240 vs. TAIR 10
Match: AT3G14420.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 429.9 bits (1104), Expect = 2.1e-120
Identity = 220/358 (61.45%), Postives = 278/358 (77.65%), Query Frame = 0

Query: 4   EPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMS 63
           E  NV +Y  +A+Q LPKM YDYYA GAEDQ TL+EN  AF RI+ RPRIL+DVSKIDM+
Sbjct: 2   EITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMT 61

Query: 64  TTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCN 123
           TT+LG++IS PIMVAPT   K+AH +GE ATARAA+AA TIM LS  ++ S+EEVAS+  
Sbjct: 62  TTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 124 AVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP---LK 183
            +RFFQLYV++ R++   LV+RAER+G+KAI LT DTPRLGRRE+DIKN+   PP   LK
Sbjct: 122 GIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLK 181

Query: 184 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 243
           N EGL   ++     S L S+V    D +L W+D+ WL++IT LPIL+KG+LT EDA  A
Sbjct: 182 NFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDARIA 241

Query: 244 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 303
           ++ G  GIIVSNHGARQLD+ PAT+S LEEVV A +G++PV LDGGVRRGTDVFKALALG
Sbjct: 242 IQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALG 301

Query: 304 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 359
           A  + IGRPV++ LAA+GE GVR VL+ML++E E+TMALSGC S+K+I+R+ + T +D
Sbjct: 302 ASGIFIGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWD 359

BLAST of MS013240 vs. TAIR 10
Match: AT3G14420.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 429.9 bits (1104), Expect = 2.1e-120
Identity = 220/358 (61.45%), Postives = 278/358 (77.65%), Query Frame = 0

Query: 4   EPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMS 63
           E  NV +Y  +A+Q LPKM YDYYA GAEDQ TL+EN  AF RI+ RPRIL+DVSKIDM+
Sbjct: 2   EITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMT 61

Query: 64  TTILGYRISAPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCN 123
           TT+LG++IS PIMVAPT   K+AH +GE ATARAA+AA TIM LS  ++ S+EEVAS+  
Sbjct: 62  TTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 124 AVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP---LK 183
            +RFFQLYV++ R++   LV+RAER+G+KAI LT DTPRLGRRE+DIKN+   PP   LK
Sbjct: 122 GIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLK 181

Query: 184 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 243
           N EGL   ++     S L S+V    D +L W+D+ WL++IT LPIL+KG+LT EDA  A
Sbjct: 182 NFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDARIA 241

Query: 244 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 303
           ++ G  GIIVSNHGARQLD+ PAT+S LEEVV A +G++PV LDGGVRRGTDVFKALALG
Sbjct: 242 IQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALG 301

Query: 304 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 359
           A  + IGRPV++ LAA+GE GVR VL+ML++E E+TMALSGC S+K+I+R+ + T +D
Sbjct: 302 ASGIFIGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWD 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148211.15.8e-19799.45peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Momordica charantia... [more]
XP_022148212.15.8e-19799.45peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Momordica charantia... [more]
XP_022148216.14.9e-18093.70peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X3 [Momordica charantia... [more]
XP_038894778.14.6e-17889.65peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Benincasa hispida] ... [more]
XP_022148294.12.4e-17186.85peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Momordica charantia] >XP_02214... [more]
Match NameE-valueIdentityDescription
Q9LJH57.9e-14972.22Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q24JJ82.3e-14873.61Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8H3I41.7e-13065.75Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=3... [more]
B8B8K57.0e-12965.19Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=399... [more]
P054147.7e-12061.73Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia oleracea OX=3562 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D2A92.8e-19799.45(S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 S... [more]
A0A6J1D4P52.8e-19799.45(S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 S... [more]
A0A6J1D4Q22.4e-18093.70(S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 S... [more]
A0A6J1D4Y81.2e-17186.85(S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016982 PE=3 S... [more]
A0A1S3CCP51.2e-16884.38(S)-2-hydroxy-acid oxidase OS=Cucumis melo OX=3656 GN=LOC103499183 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14130.15.6e-15072.22Aldolase-type TIM barrel family protein [more]
AT3G14150.11.6e-14973.61Aldolase-type TIM barrel family protein [more]
AT3G14150.21.6e-14973.61Aldolase-type TIM barrel family protein [more]
AT3G14420.12.1e-12061.45Aldolase-type TIM barrel family protein [more]
AT3G14420.22.1e-12061.45Aldolase-type TIM barrel family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM01240IMPDH_2coord: 42..359
e-value: 0.0086
score: -232.7
NoneNo IPR availablePANTHERPTHR10578S -2-HYDROXY-ACID OXIDASE-RELATEDcoord: 1..355
NoneNo IPR availablePANTHERPTHR10578:SF116PEROXISOMAL (S)-2-HYDROXY-ACID OXIDASE GLO4-LIKEcoord: 1..355
NoneNo IPR availableSUPERFAMILY51395FMN-linked oxidoreductasescoord: 5..355
IPR000262FMN-dependent dehydrogenasePFAMPF01070FMN_dhcoord: 15..354
e-value: 6.4E-136
score: 452.9
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 3..364
e-value: 7.1E-163
score: 543.6
IPR012133Alpha-hydroxy acid dehydrogenase, FMN-dependentPIRSFPIRSF000138Al-hdrx_acd_dhcoord: 1..358
e-value: 7.3E-138
score: 457.6
IPR012133Alpha-hydroxy acid dehydrogenase, FMN-dependentCDDcd02809alpha_hydroxyacid_oxid_FMNcoord: 9..350
e-value: 3.66237E-160
score: 448.82
IPR008259FMN-dependent alpha-hydroxy acid dehydrogenase, active sitePROSITEPS00557FMN_HYDROXY_ACID_DH_1coord: 251..257
IPR037396FMN hydroxy acid dehydrogenase domainPROSITEPS51349FMN_HYDROXY_ACID_DH_2coord: 1..358
score: 113.679855

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS013240.1MS013240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity
molecular_function GO:0010181 FMN binding
molecular_function GO:0016491 oxidoreductase activity