MS012919 (gene) Bitter gourd (TR) v1

Overview


Name	MS012919
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	scaffold38: 442138 .. 442689 (-)
RNA-Seq Expression	MS012919
Synteny	MS012919

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGACTCATATTTTCCACTTCCAATCACAGCCATTCTCCTCATCATTCTCATCAACCTATCAAATGCAACCATAGATGACACCCAATTCATCAAAGCCACTTGTCAAACCACACCCTATCCTGATCTCTGCTTCTCCTCACTCTCCGCGGATGCCGTTTCCGTCCACGGCAGCCTCCGCTCGATGACCATCGCTGCCCTCACCGTCGCCCTCACACACACTCGCTCCGCCTCGTCGATGGTGGCGAGTTTGGCTAAGTCGAATGCACTGGCACCGCAAGAGACTTGGGCTTTGCGTGATTGCATTGAGGAGCTTGGAGACTCTGTGGAGGAGCTCCAAAGCTCAGTGGCGGAGTTGAAAGACGGTGATGGATTTCGACCCGAGACCGATGATATTCGGACGTGGGTGAGCGCTGCTCTGACCGATGACGACACGTGCATGGAGGGATTCGTCGGAGACGGCGGCGGCGTGAAGGAGTCGGTGAGAGGACTGGTGGTGGATGTTGCTCAATTGACAAGCATTGCTCTCTCGCTTGTTAGCCATCTGAAA

mRNA sequence

ATGGAAGACTCATATTTTCCACTTCCAATCACAGCCATTCTCCTCATCATTCTCATCAACCTATCAAATGCAACCATAGATGACACCCAATTCATCAAAGCCACTTGTCAAACCACACCCTATCCTGATCTCTGCTTCTCCTCACTCTCCGCGGATGCCGTTTCCGTCCACGGCAGCCTCCGCTCGATGACCATCGCTGCCCTCACCGTCGCCCTCACACACACTCGCTCCGCCTCGTCGATGGTGGCGAGTTTGGCTAAGTCGAATGCACTGGCACCGCAAGAGACTTGGGCTTTGCGTGATTGCATTGAGGAGCTTGGAGACTCTGTGGAGGAGCTCCAAAGCTCAGTGGCGGAGTTGAAAGACGGTGATGGATTTCGACCCGAGACCGATGATATTCGGACGTGGGTGAGCGCTGCTCTGACCGATGACGACACGTGCATGGAGGGATTCGTCGGAGACGGCGGCGGCGTGAAGGAGTCGGTGAGAGGACTGGTGGTGGATGTTGCTCAATTGACAAGCATTGCTCTCTCGCTTGTTAGCCATCTGAAA

Coding sequence (CDS)

ATGGAAGACTCATATTTTCCACTTCCAATCACAGCCATTCTCCTCATCATTCTCATCAACCTATCAAATGCAACCATAGATGACACCCAATTCATCAAAGCCACTTGTCAAACCACACCCTATCCTGATCTCTGCTTCTCCTCACTCTCCGCGGATGCCGTTTCCGTCCACGGCAGCCTCCGCTCGATGACCATCGCTGCCCTCACCGTCGCCCTCACACACACTCGCTCCGCCTCGTCGATGGTGGCGAGTTTGGCTAAGTCGAATGCACTGGCACCGCAAGAGACTTGGGCTTTGCGTGATTGCATTGAGGAGCTTGGAGACTCTGTGGAGGAGCTCCAAAGCTCAGTGGCGGAGTTGAAAGACGGTGATGGATTTCGACCCGAGACCGATGATATTCGGACGTGGGTGAGCGCTGCTCTGACCGATGACGACACGTGCATGGAGGGATTCGTCGGAGACGGCGGCGGCGTGAAGGAGTCGGTGAGAGGACTGGTGGTGGATGTTGCTCAATTGACAAGCATTGCTCTCTCGCTTGTTAGCCATCTGAAA

Protein sequence

MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELKDGDGFRPETDDIRTWVSAALTDDDTCMEGFVGDGGGVKESVRGLVVDVAQLTSIALSLVSHLK

Homology

[Close]

BLAST of MS012919 vs. NCBI nr
Match: XP_022152813.1 (pectinesterase inhibitor 7-like [Momordica charantia])

HSP 1 Score: 344.0 bits (881), Expect = 8.2e-91
Identity = 182/184 (98.91%), Postives = 182/184 (98.91%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSL 60
           MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSL
Sbjct: 1   MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSL 60

Query: 61  RSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL 120
           RSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL
Sbjct: 61  RSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL 120

Query: 121 KDGDGFRPETDDIRTWVSAALTDDDTCMEGFVGDGGGVKESVRGLVVDVAQLTSIALSLV 180
           KDGDG RPETDDIRTWVSAALTDDDTCMEG VGDGGGVKESVRGLVVDVAQLTSIALSLV
Sbjct: 121 KDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDGGGVKESVRGLVVDVAQLTSIALSLV 180

Query: 181 SHLK 185
           SHLK
Sbjct: 181 SHLK 184

[Close]

BLAST of MS012919 vs. NCBI nr
Match: XP_038876812.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 241.5 bits (615), Expect = 5.7e-60
Identity = 133/190 (70.00%), Postives = 151/190 (79.47%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLIILINLSNAT----IDDTQFIKATCQTTPYPDLCFSSLSADAVSV 60
           ME SY PLPI AILLIILIN SN      I+DTQFIK TCQ+TPYP LC SSLSADA ++
Sbjct: 1   MESSYLPLPIKAILLIILINQSNIAKSQPINDTQFIKTTCQSTPYPSLCLSSLSADAATI 60

Query: 61  HGSLRSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSS 120
           HGS R MTIAALTVALT TRSASS++ SLAKSN L P+E + + DCIEE GDS+EEL+ +
Sbjct: 61  HGSYRLMTIAALTVALTRTRSASSVIQSLAKSNVLTPREGYVVSDCIEEFGDSIEELKMA 120

Query: 121 VAELKDGDGFRPETDDIRTWVSAALTDDDTCMEGFVGD--GGGVKESVRGLVVDVAQLTS 180
           + ELKD D  R E +DIRTWVSAALTDDDTCM+GFVGD     VKES++ LVVD+ QLTS
Sbjct: 121 IVELKDNDKMRFEIEDIRTWVSAALTDDDTCMDGFVGDTMNDSVKESMKKLVVDIVQLTS 180

Query: 181 IALSLVSHLK 185
           IALSLVS LK
Sbjct: 181 IALSLVSLLK 190

[Close]

BLAST of MS012919 vs. NCBI nr
Match: XP_004147977.1 (21 kDa protein [Cucumis sativus])

HSP 1 Score: 235.3 bits (599), Expect = 4.1e-58
Identity = 130/192 (67.71%), Postives = 153/192 (79.69%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLIILINLSN-----ATIDDTQFIKATCQTTPYPDLCFSSLSADAVS 60
           M+ SYFPLP+ AILLI+LIN SN       I+DTQFIK TCQ+TPYPDLC SSLS  A +
Sbjct: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60

Query: 61  VHGSLRSMTIAALTVALTHTRSASSMVASLAK-SNALAPQETWALRDCIEELGDSVEELQ 120
           +H S   MT+AALTVALTHTRS SS + SLAK SNAL P++++ +RDCIEE GDSVEEL+
Sbjct: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120

Query: 121 SSVAELKDGDGFRPETDDIRTWVSAALTDDDTCMEGFVGD--GGGVKESVRGLVVDVAQL 180
            +V ELKD +  R ET+DIRTWVSAALTDDDTCM+G VGD   G VKES++ +VV+VAQL
Sbjct: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180

Query: 181 TSIALSLVSHLK 185
           TSIALSLVS LK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of MS012919 vs. NCBI nr
Match: XP_008448897.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 233.0 bits (593), Expect = 2.0e-57
Identity = 132/192 (68.75%), Postives = 152/192 (79.17%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLI-ILINLSNAT----IDDTQFIKATCQTTPYPDLCFSSLSADAVS 60
           M+ SYFPLP+ AILL+ ILIN SN       +DTQFIK TCQ+TPYPDLC SSLS  A +
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60

Query: 61  VHGSLRSMTIAALTVALTHTRSASSMVASLAK-SNALAPQETWALRDCIEELGDSVEELQ 120
           +H S R MTIAALTVALTHTRS SS V SLAK SNAL P+E + +RDCIEE GDSVEEL+
Sbjct: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120

Query: 121 SSVAELKDGDGFRPETDDIRTWVSAALTDDDTCMEGFVGD--GGGVKESVRGLVVDVAQL 180
            ++ ELKD +  R ET+DIRTWVSAALTDDDTCM+G VGD   G +KES++ +VVDVAQL
Sbjct: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180

Query: 181 TSIALSLVSHLK 185
           TSIALSLVS LK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of MS012919 vs. NCBI nr
Match: XP_023551923.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 224.6 bits (571), Expect = 7.2e-55
Identity = 128/183 (69.95%), Postives = 143/183 (78.14%), Query Frame = 0


Query: 8   LPITAILLIILIN----LSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSM 67
           LPITAIL +ILIN    ++   I+DTQFIK TCQTTPYPDLC SSLSADA ++HGS R M
Sbjct: 6   LPITAILFMILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSADASAIHGSHRLM 65

Query: 68  TIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELKDG 127
           TIAAL VALTHT  ASS V SLAKSNAL P+E +AL DCIEE GDS+EEL+ +V ELK  
Sbjct: 66  TIAALAVALTHTHDASSTVTSLAKSNALTPREGYALSDCIEEFGDSIEELKMAVEELKGN 125

Query: 128 DGFRPETDDIRTWVSAALTDDDTCMEGFVGD--GGGVKESVRGLVVDVAQLTSIALSLVS 185
           D  R +  DI+TWVSAALTDD+TCM+GF  D   G VKESVR LVVDVAQLTSIALSLV 
Sbjct: 126 DKTRLDIKDIQTWVSAALTDDNTCMDGFAEDAMNGSVKESVRALVVDVAQLTSIALSLVR 185

[Close]

BLAST of MS012919 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.8e-25
Identity = 69/184 (37.50%), Postives = 109/184 (59.24%), Query Frame = 0


Query: 6   FPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTI 65
           F L ++   +++  NL   +  DT+FIKA+C+TT YPD CF SLS+ A  +    R +  
Sbjct: 12  FVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAE 71

Query: 66  AALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELK---- 125
            AL V++   +SA + V+ +     +  ++  A+ DC+EE+GD+V+ L +S+ ELK    
Sbjct: 72  TALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEE 131

Query: 126 --DGDGFRPETDDIRTWVSAALTDDDTCMEGFVGD--GGGVKESVRGLVVDVAQLTSIAL 182
              G+ F     ++RTW SAALTD+  CM+GF G    G +K  +R  +V VA+ TS AL
Sbjct: 132 GDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNAL 191

[Close]

BLAST of MS012919 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 6.2e-25
Identity = 74/180 (41.11%), Postives = 110/180 (61.11%), Query Frame = 0


Query: 10  ITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTIAALT 69
           + + LL+IL   S A  +  QFIK +C  T YP +C  SLSA A ++  + + +   AL 
Sbjct: 9   VFSALLVILAAASAAPAN--QFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQ 68

Query: 70  VALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELKD-----GD 129
           V+LT T+ A + +  L K   L  ++  A+ DC+EE+ DS++ +  S  E+K+     G+
Sbjct: 69  VSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGN 128

Query: 130 GFRPETDDIRTWVSAALTDDDTCMEGFVGDG--GGVKESVRGLVVDVAQLTSIALSLVSH 183
            F     ++ TWVSAALTD+ TCM+GF G G  G +KESVR  VV VA++TS AL+LV++
Sbjct: 129 DFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVNN 186

[Close]

BLAST of MS012919 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 8.1e-25
Identity = 69/165 (41.82%), Postives = 100/165 (60.61%), Query Frame = 0


Query: 22  SNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTIAALTVALTHTRSASSM 81
           SN T  +  +IK +C  T Y  +C++SLS  A ++  + + + + AL + L+  +SAS  
Sbjct: 140 SNQT--NLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKF 199

Query: 82  VASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL-----KDGDGFRPETDDIRTW 141
           V +++    L   E  A+ DC+EE+GDSV  LQ S+ EL     KD   F     D+ TW
Sbjct: 200 VKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETW 259

Query: 142 VSAALTDDDTCMEGFVGDGGGVKESVRGLVVDVAQLTSIALSLVS 182
           VSAALT+DDTCM+GF      VK+ VR  VV+VA+LTS AL+L++
Sbjct: 260 VSAALTNDDTCMDGFSLVKTAVKDLVRRHVVEVARLTSNALALIN 302

[Close]

BLAST of MS012919 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.1e-21
Identity = 74/185 (40.00%), Postives = 108/185 (58.38%), Query Frame = 0


Query: 10  ITAILLIILINLSNAT---IDDTQFIKATCQTTPYPDLCFSSLSADAVSV-HGSLRSMTI 69
           +  +L I ++  S+AT    +  QFI ++CQTT YP LC  +LSA A  + H + + +  
Sbjct: 9   LVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQ 68

Query: 70  AALTVALTHTRSASSMVASLAKSN-ALAPQETWALRDCIEELGDSVEELQSSVAELK--- 129
            AL ++L   +S +  VA L K       +E  A++DCIE LG+SV+ L  SV EL    
Sbjct: 69  TALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAG 128

Query: 130 ---DGDGFRPETDDIRTWVSAALTDDDTCMEGFV--GDGGGVKESVRGLVVDVAQLTSIA 182
                + F  +  +++TWVSAALTD+ TC++GF     GG VK  +R  VV VAQ+TS A
Sbjct: 129 HAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNA 188

[Close]

BLAST of MS012919 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.3e-19
Identity = 60/160 (37.50%), Postives = 94/160 (58.75%), Query Frame = 0


Query: 32  IKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTIAALTVALTHTRSASSMVASLAKSNAL 91
           + ++C+   YP LC  +LS+ +     + R +  AA+ ++L+H +SA+  +A +  S   
Sbjct: 34  VHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAVVRDSVGK 93

Query: 92  APQETWALRDCIEELGDSVEELQSSVAELK-------DGDGFRPETDDIRTWVSAALTDD 151
             QE  AL DC+E +GDSV+EL  ++  LK           FR +  + +TW SAALTDD
Sbjct: 94  KKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWASAALTDD 153

Query: 152 DTCMEGFVG-DGGGVKESVRGLVVDVAQLTSIALSLVSHL 184
           DTC++GF G D G +K  V+  +  VA++TS AL +V+ L
Sbjct: 154 DTCLDGFQGMDDGEIKTEVKQWMTKVARVTSNALYMVNQL 193

[Close]

BLAST of MS012919 vs. ExPASy TrEMBL
Match: A0A6J1DFW8 (pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 3.9e-91
Identity = 182/184 (98.91%), Postives = 182/184 (98.91%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSL 60
           MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSL
Sbjct: 1   MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSL 60

Query: 61  RSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL 120
           RSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL
Sbjct: 61  RSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL 120

Query: 121 KDGDGFRPETDDIRTWVSAALTDDDTCMEGFVGDGGGVKESVRGLVVDVAQLTSIALSLV 180
           KDGDG RPETDDIRTWVSAALTDDDTCMEG VGDGGGVKESVRGLVVDVAQLTSIALSLV
Sbjct: 121 KDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDGGGVKESVRGLVVDVAQLTSIALSLV 180

Query: 181 SHLK 185
           SHLK
Sbjct: 181 SHLK 184

[Close]

BLAST of MS012919 vs. ExPASy TrEMBL
Match: A0A0A0L1P3 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 2.0e-58
Identity = 130/192 (67.71%), Postives = 153/192 (79.69%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLIILINLSN-----ATIDDTQFIKATCQTTPYPDLCFSSLSADAVS 60
           M+ SYFPLP+ AILLI+LIN SN       I+DTQFIK TCQ+TPYPDLC SSLS  A +
Sbjct: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60

Query: 61  VHGSLRSMTIAALTVALTHTRSASSMVASLAK-SNALAPQETWALRDCIEELGDSVEELQ 120
           +H S   MT+AALTVALTHTRS SS + SLAK SNAL P++++ +RDCIEE GDSVEEL+
Sbjct: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120

Query: 121 SSVAELKDGDGFRPETDDIRTWVSAALTDDDTCMEGFVGD--GGGVKESVRGLVVDVAQL 180
            +V ELKD +  R ET+DIRTWVSAALTDDDTCM+G VGD   G VKES++ +VV+VAQL
Sbjct: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180

Query: 181 TSIALSLVSHLK 185
           TSIALSLVS LK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of MS012919 vs. ExPASy TrEMBL
Match: A0A1S3BLP3 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 9.8e-58
Identity = 132/192 (68.75%), Postives = 152/192 (79.17%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLI-ILINLSNAT----IDDTQFIKATCQTTPYPDLCFSSLSADAVS 60
           M+ SYFPLP+ AILL+ ILIN SN       +DTQFIK TCQ+TPYPDLC SSLS  A +
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60

Query: 61  VHGSLRSMTIAALTVALTHTRSASSMVASLAK-SNALAPQETWALRDCIEELGDSVEELQ 120
           +H S R MTIAALTVALTHTRS SS V SLAK SNAL P+E + +RDCIEE GDSVEEL+
Sbjct: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120

Query: 121 SSVAELKDGDGFRPETDDIRTWVSAALTDDDTCMEGFVGD--GGGVKESVRGLVVDVAQL 180
            ++ ELKD +  R ET+DIRTWVSAALTDDDTCM+G VGD   G +KES++ +VVDVAQL
Sbjct: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180

Query: 181 TSIALSLVSHLK 185
           TSIALSLVS LK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of MS012919 vs. ExPASy TrEMBL
Match: A0A6J1E6H9 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 1.1e-51
Identity = 120/183 (65.57%), Postives = 141/183 (77.05%), Query Frame = 0


Query: 8   LPITAILLIILIN----LSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSM 67
           LPITAI+ +ILIN    ++   I+DTQFIK TCQTTPYPDLC SSLS DA ++  S R M
Sbjct: 6   LPITAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSHRLM 65

Query: 68  TIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELKDG 127
           TIAAL VALTHT  ASS V SLAKSNA+  +E +AL DCIEE GDSVEEL+ +V  LK  
Sbjct: 66  TIAALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVEALKGN 125

Query: 128 DGFRPETDDIRTWVSAALTDDDTCMEGFVGD--GGGVKESVRGLVVDVAQLTSIALSLVS 185
           D  R +T+DI+TW+SAALTDD+TCM+GF GD   G VKES++ +VVDVAQLTSIALSLV 
Sbjct: 126 DKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSLVR 185

[Close]

BLAST of MS012919 vs. ExPASy TrEMBL
Match: A0A5D3D7I0 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001400 PE=4 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.6e-36
Identity = 91/125 (72.80%), Postives = 104/125 (83.20%), Query Frame = 0


Query: 63  MTIAALTVALTHTRSASSMVASLAK-SNALAPQETWALRDCIEELGDSVEELQSSVAELK 122
           MTIAALTVALTHTRS SS V SLAK SNAL P+E + +RDCIEE GDSVEEL+ ++ ELK
Sbjct: 1   MTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK 60

Query: 123 DGDGFRPETDDIRTWVSAALTDDDTCMEGFVGD--GGGVKESVRGLVVDVAQLTSIALSL 182
           D +  R ET+DIRTWVSAALTDDDTCM+G VGD   G +KES++ +VVDVAQLTSIALSL
Sbjct: 61  DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSL 120

Query: 183 VSHLK 185
           VS LK
Sbjct: 121 VSLLK 125

[Close]

BLAST of MS012919 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-29
Identity = 77/183 (42.08%), Postives = 114/183 (62.30%), Query Frame = 0


Query: 11  TAILLIILINL-------SNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSM 70
           T +++I ++ L       +  T  +T+F+K++C  T YP LCFSSLS  A  +  S + M
Sbjct: 16  TFLIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLM 75

Query: 71  TIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL--K 130
             AAL + L   +  S+M+  L+ S  L P+E  A+RDC+EELGD++EEL+ S+ E+   
Sbjct: 76  AHAALNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQL 135

Query: 131 DGDGFRPETDDIRTWVSAALTDDDTCMEGFVGD--GGGVKESVRGLVVDVAQLTSIALSL 183
            G  +     DI+TWVSAALTD +TC +GF G+   G VK  VRG ++ +A LTS AL+L
Sbjct: 136 SGSNYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALAL 195

[Close]

BLAST of MS012919 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 2.0e-26
Identity = 69/184 (37.50%), Postives = 109/184 (59.24%), Query Frame = 0


Query: 6   FPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTI 65
           F L ++   +++  NL   +  DT+FIKA+C+TT YPD CF SLS+ A  +    R +  
Sbjct: 12  FVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAE 71

Query: 66  AALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELK---- 125
            AL V++   +SA + V+ +     +  ++  A+ DC+EE+GD+V+ L +S+ ELK    
Sbjct: 72  TALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEE 131

Query: 126 --DGDGFRPETDDIRTWVSAALTDDDTCMEGFVGD--GGGVKESVRGLVVDVAQLTSIAL 182
              G+ F     ++RTW SAALTD+  CM+GF G    G +K  +R  +V VA+ TS AL
Sbjct: 132 GDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNAL 191

[Close]

BLAST of MS012919 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 115.2 bits (287), Expect = 5.7e-26
Identity = 69/165 (41.82%), Postives = 100/165 (60.61%), Query Frame = 0


Query: 22  SNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTIAALTVALTHTRSASSM 81
           SN T  +  +IK +C  T Y  +C++SLS  A ++  + + + + AL + L+  +SAS  
Sbjct: 140 SNQT--NLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKF 199

Query: 82  VASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL-----KDGDGFRPETDDIRTW 141
           V +++    L   E  A+ DC+EE+GDSV  LQ S+ EL     KD   F     D+ TW
Sbjct: 200 VKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETW 259

Query: 142 VSAALTDDDTCMEGFVGDGGGVKESVRGLVVDVAQLTSIALSLVS 182
           VSAALT+DDTCM+GF      VK+ VR  VV+VA+LTS AL+L++
Sbjct: 260 VSAALTNDDTCMDGFSLVKTAVKDLVRRHVVEVARLTSNALALIN 302

[Close]

BLAST of MS012919 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 5.9e-23
Identity = 63/159 (39.62%), Postives = 93/159 (58.49%), Query Frame = 0


Query: 31  FIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTIAALTVALTHTRSASSMVASLAKSNA 90
           FI+++C+TT YP LC  SLS  A  +  S + +   A+ V L+  +S    V+ L +   
Sbjct: 38  FIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKG 97

Query: 91  LAPQETWALRDCIEELGDSVEELQSSVAEL------KDGDGFRPETDDIRTWVSAALTDD 150
           L  +E  A++DC+EE+ D+V+ L  SV EL      KD D F     + +TW SAALTD+
Sbjct: 98  LKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDE 157

Query: 151 DTCMEGFVGD--GGGVKESVRGLVVDVAQLTSIALSLVS 182
           +TC +GF G    G +K SVR  +++V   TS ALSL++
Sbjct: 158 NTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLIN 196

[Close]

BLAST of MS012919 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 7.8e-23
Identity = 74/185 (40.00%), Postives = 108/185 (58.38%), Query Frame = 0


Query: 10  ITAILLIILINLSNAT---IDDTQFIKATCQTTPYPDLCFSSLSADAVSV-HGSLRSMTI 69
           +  +L I ++  S+AT    +  QFI ++CQTT YP LC  +LSA A  + H + + +  
Sbjct: 9   LVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQ 68

Query: 70  AALTVALTHTRSASSMVASLAKSN-ALAPQETWALRDCIEELGDSVEELQSSVAELK--- 129
            AL ++L   +S +  VA L K       +E  A++DCIE LG+SV+ L  SV EL    
Sbjct: 69  TALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAG 128

Query: 130 ---DGDGFRPETDDIRTWVSAALTDDDTCMEGFV--GDGGGVKESVRGLVVDVAQLTSIA 182
                + F  +  +++TWVSAALTD+ TC++GF     GG VK  +R  VV VAQ+TS A
Sbjct: 129 HAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNA 188

The following BLAST results are available for this feature:

BLAST of MS012919 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152813.1	8.2e-91	98.91	pectinesterase inhibitor 7-like [Momordica charantia]	[more]
XP_038876812.1	5.7e-60	70.00	21 kDa protein-like [Benincasa hispida]	[more]
XP_004147977.1	4.1e-58	67.71	21 kDa protein [Cucumis sativus]	[more]
XP_008448897.1	2.0e-57	68.75	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
XP_023551923.1	7.2e-55	69.95	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS012919 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SB37	2.8e-25	37.50	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
P17407	6.2e-25	41.11	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SI74	8.1e-25	41.82	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9SI72	1.1e-21	40.00	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q84WE4	2.3e-19	37.50	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]

BLAST of MS012919 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DFW8	3.9e-91	98.91	pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 P...	[more]
A0A0A0L1P3	2.0e-58	67.71	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 S...	[more]
A0A1S3BLP3	9.8e-58	68.75	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1	[more]
A0A6J1E6H9	1.1e-51	65.57	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1	[more]
A0A5D3D7I0	1.6e-36	72.80	21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G...	[more]

BLAST of MS012919 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62360.1	1.5e-29	42.08	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	2.0e-26	37.50	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	5.7e-26	41.82	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	5.9e-23	39.62	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62770.1	7.8e-23	40.00	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 103..123
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 23..183
None	No IPR available	PANTHER	PTHR31080:SF161	21 KDA PROTEIN-LIKE	coord: 23..183
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 34..183 e-value: 5.73965E-45 score: 144.118
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 27..179 e-value: 6.9E-48 score: 175.1
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 31..179 e-value: 5.2E-37 score: 127.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..183 e-value: 3.0E-35 score: 119.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 26..184 e-value: 3.8E-41 score: 142.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 30..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012919.1	MS012919.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004857	enzyme inhibitor activity