MS012763 (gene) Bitter gourd (TR) v1

BLAST of MS012763 vs. NCBI nr
Match: XP_022159381.1 (subtilisin-like protease SBT3.4, partial [Momordica charantia])

HSP 1 Score: 992.3 bits (2564), Expect = 2.2e-285
Identity = 505/607 (83.20%), Postives = 539/607 (88.80%), Query Frame = 0

Query: 35  MPGVVRVLPSTIYKMQTTRSWDFLGLSSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESE 94
           M GVVRVLP+++YKMQTTRSWDFLGLS+SPSQSSNLLHRGKMGE V+IGV+D+GIWPESE
Sbjct: 1   MAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGEHVIIGVLDSGIWPESE 60

Query: 95  AFGDEGMGPVPSRWKGKCESGAEFNSTNCNRKIIGARWYNKGFIVDVGQEALANEYLSAR 154
           AF DEGMGPVPSRWKGKCESG EFNSTNCNRKIIGARWYNKG I D GQ ALANEY SAR
Sbjct: 61  AFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGXIADXGQXALANEYXSAR 120

Query: 155 DFQGHGTHVASTAAGSFVPNVNYQGLGAGRVRGGAPRARLAIYKAFWSAAGQGSAADILM 214
           DF GHGTH ASTAAGSFVPNVNYQGLGAG VRGGAPRA LAIYK  WS  G GS ADILM
Sbjct: 121 DFXGHGTHXASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLWS-EGAGSGADILM 180

Query: 215 AIDQAIHDGVDVLSLSLTVGRVPISPEFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQ 274
           A+D AIHDGVDVLSLSL  G VPISPEFSEDSVVAIG+FHATARGISVVCAGGNDGP+KQ
Sbjct: 181 AMDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQ 240

Query: 275 TILNTAPWIFTVAASTMDRAFVSSITLGDDTTHLGQSLFTAKNDIGGNLVCS--NSLCSC 334
           TI+NTAPWIFTVAAST+DRAF++ +TLGD+TTHLGQS F  +NDI G LVCS    LCSC
Sbjct: 241 TIVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSC 300

Query: 335 EGILRNDTSLSGNVVLCFSKLANVATLLVAAATVEAANGTGIIVAGQHDDILVPCGQARQ 394
           + IL NDTSLSGN+VLCF+KL ++     A   V  ANG GIIVAGQHDDIL PCG    
Sbjct: 301 KDILGNDTSLSGNIVLCFNKLVDLEMTQKAVVAVRQANGNGIIVAGQHDDILFPCG--AD 360

Query: 395 FPCIVVDPDVATKIYHYFWDVTNPTVTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAI 454
           FPCIV+DPDV TK +HY  D +NP   VR+GRA T+VGKPISTN+AFFSSRGPNSASPAI
Sbjct: 361 FPCIVLDPDVGTKFFHYXLDTSNP--MVRIGRAXTIVGKPISTNVAFFSSRGPNSASPAI 420

Query: 455 LKPDIAAPGSNILAAVPPHHRFSDKGFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIR 514
           LKPDIAAPGSNILAA+ P + FSDKGFAF SGTSMAAPHISGIVALLKS+HPTWSPAAIR
Sbjct: 421 LKPDIAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIR 480

Query: 515 SAISTTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYY 574
           SA+STTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYY
Sbjct: 481 SAJSTTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYY 540

Query: 575 LCGMGYKNSDISHLTEQKTLCPSERSSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSV 634
           LCGMGYKNSDIS LTEQKT CPS+RSSVLDLNLPSIT+PALRNSTTVTRTVTNVGN SSV
Sbjct: 541 LCGMGYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSV 600

Query: 635 YRAVIRA 640
           YRAVIRA
Sbjct: 601 YRAVIRA 602

BLAST of MS012763 vs. NCBI nr
Match: XP_004146562.1 (subtilisin-like protease SBT3.6 [Cucumis sativus] >KGN53334.1 hypothetical protein Csa_014762 [Cucumis sativus])

HSP 1 Score: 896.3 bits (2315), Expect = 1.6e-256
Identity = 458/701 (65.34%), Postives = 551/701 (78.60%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKEKS E+MVYSY+HGFSGFAAKLTKSQAQKL+EM  VVRV+PS++YK+ TTRSWDFLGL
Sbjct: 65  SKEKSMEAMVYSYKHGFSGFAAKLTKSQAQKLSEMSRVVRVVPSSLYKVHTTRSWDFLGL 124

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
           SSSP +SSNLLHR +MGE+V+IGV+DTGIWPESE+F D+G+G +PSRWKG CESG +FNS
Sbjct: 125 SSSPFESSNLLHRAQMGENVIIGVIDTGIWPESESFKDKGVGSIPSRWKGTCESGEQFNS 184

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGL 180
           TNCN+KIIGARW+ KGF+ D+G++ALA EYLS RD  GHGTH AS AAGSFV N+NY   
Sbjct: 185 TNCNKKIIGARWFMKGFVADLGRDALAKEYLSPRDLNGHGTHTASIAAGSFVANINYHNN 244

Query: 181 GAGRVRGGAPRARLAIYKAFWSAAGQGSAADILMAIDQAIHDGVDVLSLSLTVGRVPISP 240
            AG VRGGAP ARLAIYKA W+    GS ADIL AID+AI+DGVDVLS+S+     P  P
Sbjct: 245 AAGTVRGGAPLARLAIYKALWTKDAVGSTADILKAIDEAINDGVDVLSMSIG-SLTPFLP 304

Query: 241 EFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRAFVSSI- 300
           EF+E + +A GSFHA A+GISVVCA GN GP  QT+ N APWIFTVAA+T+DRAF++SI 
Sbjct: 305 EFNEANDIAFGSFHAIAKGISVVCAAGNSGPTPQTVENVAPWIFTVAANTIDRAFLASIT 364

Query: 301 TLGDDTTHLGQSLFTAKNDIGGNLVCSNSLCSCEGILRNDTSLSGNVVLCFSKLANVATL 360
           TL D+TT LGQSL  +K D+   L   ++   C+ +L N+T ++G VV+CFS LA+  T+
Sbjct: 365 TLPDNTTFLGQSLLDSKKDLVAELETLDT-GRCDDLLGNETFINGKVVMCFSNLADHNTI 424

Query: 361 LVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATKIY--HYFWDVTNPT 420
             AA  V  ANGTGIIVAGQ DD L  C  +   PCI+VD DV +K++  +   + TNP 
Sbjct: 425 YDAAMAVARANGTGIIVAGQQDDDLFSCIPS-PIPCILVDTDVGSKLFFINLLQNSTNPV 484

Query: 421 VTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNILAAVPPHHRFSDK 480
           V +R  R  T++GKPI+  I++FSSRGPNS S  ILKPDI+APGSNILAAV PHH F++K
Sbjct: 485 VRLRATR--TIIGKPITPAISYFSSRGPNSVSNPILKPDISAPGSNILAAVSPHHIFNEK 544

Query: 481 GFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPSGAPILAESSPPK 540
           GF   SGTSMA PHIS IVALLKS+HPTWSPAAI+SA+ TTAR +   G PI AE +PPK
Sbjct: 545 GFMLLSGTSMATPHISAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFAEGTPPK 604

Query: 541 LADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISHLTEQKTLCPSER 600
           +ADPFDYGGG+V+ NAA DPGL+YD+   DY+ YYLCGMGYK+ DISHLT++KT+CP +R
Sbjct: 605 MADPFDYGGGIVDANAAVDPGLVYDMGRKDYIDYYLCGMGYKDEDISHLTQRKTVCPLQR 664

Query: 601 SSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKVNAEPSMLAFNPT 660
            SVLDLNLP+ITIP+L NST VTRTVTNVGNLS VY+A I +P+G KV+  P +L FN  
Sbjct: 665 LSVLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAEIESPFGCKVSVNPQVLVFNSQ 724

Query: 661 VKKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVR 699
           VKKISFKV     + RNYGYSFG L W+DG+H VKIPLSVR
Sbjct: 725 VKKISFKVMFFTQVQRNYGYSFGRLTWTDGIHVVKIPLSVR 760

BLAST of MS012763 vs. NCBI nr
Match: XP_022984814.1 (subtilisin-like protease SBT3.9 [Cucurbita maxima])

HSP 1 Score: 890.2 bits (2299), Expect = 1.2e-254
Identity = 456/702 (64.96%), Postives = 550/702 (78.35%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKEKS ESMVYSYRHGFSGFAAKLT SQAQKLA MP V RV PS++YKM+TTRSWDFLGL
Sbjct: 63  SKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWDFLGL 122

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
           SSSPS+SSNL HR KMG++V+IGV+DTG WPESE+F D+GMGP+PSRWKG C+SG +FNS
Sbjct: 123 SSSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDFNS 182

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGL 180
           ++CN+K+IGARW+    I D G+EA+  +YLSARD +GHGTH ASTA G+FV NV+Y G 
Sbjct: 183 SHCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVRNVSYFGN 242

Query: 181 GAGRVRGGAPRARLAIYKAFWSAAGQGSAADILMAIDQAIHDGVDVLSLSLTVGRVPISP 240
           G G +RGGAP ARLAIYK  WS +  GS ADIL  ID+AIHDGVDVLS+S+    +P+ P
Sbjct: 243 GRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGKS-IPLFP 302

Query: 241 EFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRAFVSSI- 300
           + ++ + VA+GSFHA A+GISVVCAGGN+G  +QT+ N APW+FTVAAST+DRAF+ SI 
Sbjct: 303 DVNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLVSIT 362

Query: 301 TLGDDTTHLGQSLFTAKNDIGGNLVCSNSLCSCEGILRNDTSLSGNVV-LCFSKLANVAT 360
           TLGD+ T+LGQ     + DI G LV  +  C+  GIL  D  +SGNVV LCF+ LA  A 
Sbjct: 363 TLGDNATYLGQMFL--RKDIVGMLVAMDGRCA--GILGGDIPISGNVVLLCFTDLAKTAP 422

Query: 361 LLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATKIYHYFWDVTNPTV 420
              A    + A   G+I AGQ  D LVPC      PCI VD  V TK++ Y   + +   
Sbjct: 423 ASNAVMPGKQAKVVGVIYAGQQTDNLVPC----DIPCIHVDTHVGTKLFTYL--LNDDDA 482

Query: 421 TVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNILAAVPPHHRFSDKG 480
            +RL    T++GKPIS+ IA+FSSRGPNS SP ILKPDIAAPG+NI+AAVPP+H+  DKG
Sbjct: 483 LIRLRATRTIIGKPISSRIAYFSSRGPNSFSPGILKPDIAAPGANIIAAVPPNHKGGDKG 542

Query: 481 FAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPSGAPILAESSPPKL 540
           FA  SGTSMA PHISGIV L+KS+HPTWSPAAI+SA+ TTARV+ PSG PI AE SPPK+
Sbjct: 543 FATMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSPPKV 602

Query: 541 ADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISHLTEQKTLCPSERS 600
           ADPFDYGGG+V+ NAA DPGLIYDL   DY+ YYLC MGY  +DISHL++QKT+CPS+R+
Sbjct: 603 ADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRA 662

Query: 601 SVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKVNAEPSMLAFNPTV 660
           S+LDLNLP+IT+PAL NSTTVTRTVTNVGNL++VY+AVI+AP G+KV  +P +L FN  V
Sbjct: 663 SILDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNV 722

Query: 661 KKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTD 701
           KKISFKV  S+++ RNYGYSFGSL W+DGVH VK PLSVR D
Sbjct: 723 KKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 753

BLAST of MS012763 vs. NCBI nr
Match: XP_023552781.1 (subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 889.4 bits (2297), Expect = 2.0e-254
Identity = 460/702 (65.53%), Postives = 554/702 (78.92%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKEKS ES+VYSYRHGFSGFAAKLT SQAQKLA MP V RV PS +YKM+TTRSWDFLGL
Sbjct: 65  SKEKSLESIVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRTTRSWDFLGL 124

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
           SSSPS SSNLLHR KMG++V+IGV+DTG WPESE+F D+GMGP+PSRWKG CESG +FNS
Sbjct: 125 SSSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICESGEDFNS 184

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGL 180
           ++CN+K+IGARW+    + D G+EA+  +YLSARD QGHGTH ASTA G+FV NV+  G 
Sbjct: 185 SHCNKKVIGARWFASALVADHGEEAVFMDYLSARDNQGHGTHTASTAGGAFVRNVSLFGN 244

Query: 181 GAGRVRGGAPRARLAIYKAFWSAAGQGSAADILMAIDQAIHDGVDVLSLSLTVGRVPISP 240
           G G +RGGAP ARLAIYK  WS +  GS ADIL  ID+AIHDGVDVLS+S+    +P+  
Sbjct: 245 GRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGKS-IPLFS 304

Query: 241 EFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRAFVSSI- 300
           + +E + VA+GSFHA A+GISVVCAGGN+G  +QT+ N APW+FTVAAST+DRAF++SI 
Sbjct: 305 DVNELNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLASIT 364

Query: 301 TLGDDTTHLGQSLFTAKNDIGGNLVCSNSLCSCEGILRNDTSLSGNVV-LCFSKLANVAT 360
           TLGD+ T+LGQ+    K DI G L+  +  C+  G+L ++  +SGNVV LCF+ LA  A 
Sbjct: 365 TLGDNATYLGQTFL--KKDIVGMLLAMDGRCA--GLLGSNIHISGNVVLLCFTDLAKKAG 424

Query: 361 LLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATKIYHYFWDVTNPTV 420
                   + A   GII AGQH+DIL PC      PCI VD  V TK++ Y+  + +   
Sbjct: 425 ASNVVMPGKQAKVVGIIYAGQHNDILGPC----DTPCIHVDTHVGTKLFTYY--LNDEKA 484

Query: 421 TVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNILAAVPPHHRFSDKG 480
            +RL    T+ GKPIS+ IA+FSSRGPNS SP ILKPDIAAPGSNI+AAVPP+H+ SDKG
Sbjct: 485 FIRLRATRTITGKPISSRIAYFSSRGPNSISPGILKPDIAAPGSNIIAAVPPNHKGSDKG 544

Query: 481 FAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPSGAPILAESSPPKL 540
           FA  SGTSMAAPHISGIVAL+KS+ PTWSPAAI+SA+ TTARV+ PSG PI AE SPPK+
Sbjct: 545 FAAMSGTSMAAPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKV 604

Query: 541 ADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISHLTEQKTLCPSERS 600
           ADPFDYGGG+V+ NAA DPGLIYDL   DY+ YYLC MGY  ++ISHL++QKT+CPS+R+
Sbjct: 605 ADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEAEISHLSQQKTVCPSKRA 664

Query: 601 SVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKVNAEPSMLAFNPTV 660
           SVLDLNLP+IT+PAL NSTTVTRTVTNVGNL++VY+AVI+AP G+KV  +P +LAFN  V
Sbjct: 665 SVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLAFNSNV 724

Query: 661 KKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTD 701
           KKISFKV  S+S+ RNYGYSFGSL W+DGVH VK PLSVR D
Sbjct: 725 KKISFKVKFSSSLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 755

BLAST of MS012763 vs. NCBI nr
Match: XP_022931496.1 (subtilisin-like protease SBT3.7 isoform X4 [Cucurbita moschata])

HSP 1 Score: 885.6 bits (2287), Expect = 2.9e-253
Identity = 457/702 (65.10%), Postives = 547/702 (77.92%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKEKS ESMVYSY+HGFSGFAAKLT SQAQKLA MP V RV PS++YKM TTRSWDFLGL
Sbjct: 63  SKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWDFLGL 122

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
           SSSPS SSNLLHR KMG++V+IGV+D+G WPESE+F D+GMGP+PSRWKG C+ G +FNS
Sbjct: 123 SSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGEDFNS 182

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGL 180
            +CN+K+IGARW+ +  I D G+EA+  +YLSARD +GHGTH ASTA G+FV NV+Y G 
Sbjct: 183 FHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGN 242

Query: 181 GAGRVRGGAPRARLAIYKAFWSAAGQGSAADILMAIDQAIHDGVDVLSLSLTVGRVPISP 240
             G +RGGAP ARLAIYK  WS    GSAADIL  ID+AIHDGVDVLS+S+  G +P+ P
Sbjct: 243 RRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIG-GSIPLPP 302

Query: 241 EFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRAFVSSI- 300
           + SE + VAIGSFHA ARG+SVVCAGGN+G  +QT++N APW+FTVAAST+DRAF++SI 
Sbjct: 303 DVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLTSIT 362

Query: 301 TLGDDTTHLGQSLFTAKNDIGGNLVCSNSLCSCEGILRNDTSLSGNVV-LCFSKLANVAT 360
           TLGD+ T+LGQ+    K D+ G LV  +  C+  G L +D    GNVV LCF  LA  A 
Sbjct: 363 TLGDNATYLGQTFL--KKDVVGKLVVMDRRCA--GHLGSDIPTGGNVVLLCFIDLAMKAA 422

Query: 361 LLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATKIYHYFWDVTNPTV 420
                  V+ A   G+I AGQH DIL PC      PCI VD  V TK+  Y   +++   
Sbjct: 423 ASNPVMPVKQAKVVGVIYAGQHTDILGPC----DVPCIHVDTHVGTKLLTYI--LSDEKA 482

Query: 421 TVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNILAAVPPHHRFSDKG 480
            +RL    T+VGKPIS+ IA+FSSRGPNS SP ILKPDIAAPGSNI+AAVPP+H+  DKG
Sbjct: 483 LIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKG 542

Query: 481 FAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPSGAPILAESSPPKL 540
           FA  SGTSMA PHISGIVAL+KS+ PTWSPAAI+SA+ TTARV+ PSG PI AE SPPK+
Sbjct: 543 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKV 602

Query: 541 ADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISHLTEQKTLCPSERS 600
           ADPFDYGGG+V+ NAA DPGLIYDL   DY+ YY+C MGY  ++ISHL++QK +CPS+R+
Sbjct: 603 ADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRA 662

Query: 601 SVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKVNAEPSMLAFNPTV 660
           SVLDLNLP+IT+PAL NSTTVTRTVTNVGNL++VY+AVI+AP G+KV   P +L FN  V
Sbjct: 663 SVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNV 722

Query: 661 KKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTD 701
           KKISFKV  S+++ RNYGYSFGSL W+DGVH VK PLSVR D
Sbjct: 723 KKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 753

BLAST of MS012763 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 3.1e-202
Identity = 379/717 (52.86%), Postives = 493/717 (68.76%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKE + +SMVYSYRHGFSGFAAKLT+SQA+K+A++P VV V+P + YK+ TTR+WD+LGL
Sbjct: 64  SKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGL 123

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
           S++  +S  LLH   MGE ++IGV+DTG+WPESE F D G GPVPS WKG CE+G  FNS
Sbjct: 124 SAANPKS--LLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNS 183

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALAN--EYLSARDFQGHGTHVASTAAGSFVPNVNYQ 180
           +NCN+K+IGA+++  GF+ +       N  +++S RD  GHGTHV++ A GSFVPN++Y+
Sbjct: 184 SNCNKKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYK 243

Query: 181 GLGAGRVRGGAPRARLAIYKAFWSAAGQG----SAADILMAIDQAIHDGVDVLSLSLTVG 240
           GL  G VRGGAPRA +A+YKA W          S+ADIL A+D+A+HDGVDVLS+SL   
Sbjct: 244 GLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLG-S 303

Query: 241 RVPISPEFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRA 300
            VP+  E      +  G+FHA  +GI+VVC+GGN GP+  T+ NTAPWI TVAA+T+DR+
Sbjct: 304 SVPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRS 363

Query: 301 FVSSITLGDDTTHLGQSLFTAKNDIGGNLVC------SNSLCS--CEGILRN-DTSLSGN 360
           F + +TLG++   LGQ+++T       +LV       SN   S  CE +L N + ++ G 
Sbjct: 364 FATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGK 423

Query: 361 VVLCFSKLANVATLLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATK 420
           VVLCF+       +L AA  V+ A G G+I+A      + PC     FPC+ VD ++ T 
Sbjct: 424 VVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPC--LDDFPCVAVDWELGTD 483

Query: 421 IYHYFWDVTNPTVTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNIL 480
           I  Y     +P V ++  +  T+VG+P+ T +A FSSRGPNS +PAILKPDIAAPG +IL
Sbjct: 484 ILLYTRSSGSPVVKIQPSK--TLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSIL 543

Query: 481 AAVPPHHRFSDKGFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPS 540
           AA   +  FSD+GF   SGTSMAAP ISG+ ALLK++H  WSPAAIRSAI TTA    P 
Sbjct: 544 AAT-TNTTFSDQGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPF 603

Query: 541 GAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISH 600
           G  I AE SPPKLADPFDYGGGLVNP  +A+PGL+YD+ + DYV  Y+C +GY  + IS 
Sbjct: 604 GEQIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQ 663

Query: 601 LTEQKTLCPSERSSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKV 660
           L  + T+C + + SVLD NLPSITIP L++  T+TRTVTNVG L+SVYR  +  P G +V
Sbjct: 664 LIGKTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQV 723

Query: 661 NAEPSMLAFNPTVKKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTDLL 703
              P  L FN T KK+ FKV +S +   N GY FGSL WSD +H+V IPLSVRT +L
Sbjct: 724 TVTPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 771

BLAST of MS012763 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 5.3e-202
Identity = 378/717 (52.72%), Postives = 488/717 (68.06%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SK+ + ESMVYSYRHGFSGFAAKLTKSQA+K+A+ P V+ V+P + Y++ TTR WD+LG 
Sbjct: 59  SKDDAHESMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYELATTRIWDYLG- 118

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
             S   S NL+    MG+  +IGV+DTG+WPESE+F D G+GPVPS WKG CE G  F S
Sbjct: 119 -PSADNSKNLVSDTNMGDQTIIGVIDTGVWPESESFNDYGVGPVPSHWKGGCEPGENFIS 178

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALAN-EYLSARDFQGHGTHVASTAAGSFVPNVNYQG 180
           TNCNRK+IGA+++  GF+ +    A  + +Y+SARDF GHGTHVAS A GSFVPNV+Y+G
Sbjct: 179 TNCNRKLIGAKYFINGFLAENQFNATESPDYISARDFDGHGTHVASIAGGSFVPNVSYKG 238

Query: 181 LGAGRVRGGAPRARLAIYKAFWSA----AGQGSAADILMAIDQAIHDGVDVLSLSLTVGR 240
           LG G +RGGAPRAR+A+YKA W          S +DI+ AID+AIHDGVDVLS+SL  GR
Sbjct: 239 LGRGTLRGGAPRARIAMYKACWYINELDGVTCSFSDIMKAIDEAIHDGVDVLSISLG-GR 298

Query: 241 VPISPEFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRAF 300
           VP++ E      +A G+FHA A+GI VVCAGGN GP+ QT++NTAPWI TVAA+T+DR+F
Sbjct: 299 VPLNSETDLRDGIATGAFHAVAKGIVVVCAGGNAGPSSQTVVNTAPWILTVAATTLDRSF 358

Query: 301 VSSITLGDDTTHLGQSLFTA----------KNDIGGNLVCSNSLCSCEGILRNDTSLSGN 360
            + I LG++   LGQ+++              D G ++   + +C    +  N T ++G 
Sbjct: 359 ATPIILGNNQVILGQAMYIGPELGFTSLVYPEDPGNSIDTFSGVCESLNLNSNRT-MAGK 418

Query: 361 VVLCFSKLANVATLLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATK 420
           VVLCF+   +   +  AA+ V+AA G G+I+A      L PC     FPC+ +D ++ T 
Sbjct: 419 VVLCFTTARDFTVVSTAASIVKAAGGLGLIIARNPGYNLAPCSD--DFPCVAIDNELGTD 478

Query: 421 IYHYFWDVTNPTVTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNIL 480
           I  Y     +P V ++  R  T+VG+P+ T +A FSSRGPNS SPAILKPDIAAPG +IL
Sbjct: 479 ILFYIRYTGSPVVKIQPSR--TLVGEPVGTKVATFSSRGPNSISPAILKPDIAAPGVSIL 538

Query: 481 AAVPPHHRFSDKGFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPS 540
           AA  P+   +  GF   SGTSMAAP ISG++ALLKS+HP WSPAA RSAI TTA    P 
Sbjct: 539 AATSPNDTLNAGGFVMRSGTSMAAPVISGVIALLKSLHPDWSPAAFRSAIVTTAWRTDPF 598

Query: 541 GAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISH 600
           G  I AESS  K+ DPFDYGGGLVNP  AA+PGLI D+   DYV  YLC  GY +S IS 
Sbjct: 599 GEQIAAESSSLKVPDPFDYGGGLVNPEKAAEPGLILDMDSQDYV-LYLCSAGYNDSSISR 658

Query: 601 LTEQKTLCPSERSSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKV 660
           L  + T+C + + SVLD+NLPSITIP L++  T+TRTVTNVG + SVY+ ++  P G +V
Sbjct: 659 LVGKVTVCSNPKPSVLDINLPSITIPNLKDEVTLTRTVTNVGPVDSVYKVLVEPPLGIQV 718

Query: 661 NAEPSMLAFNPTVKKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTDLL 703
              P  L FN   K +SF V +S +   N G+ FGSL W+D +H+V IP+SVRT +L
Sbjct: 719 VVTPETLVFNSKTKSVSFTVIVSTTHKINTGFYFGSLTWTDSIHNVVIPVSVRTQIL 766

BLAST of MS012763 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 1.2e-201
Identity = 383/717 (53.42%), Postives = 491/717 (68.48%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKE +  SMV+SYRHGFSGFAAKLTKSQA+KLA++P VV V P + Y++ TTR+WD+LGL
Sbjct: 60  SKEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDYLGL 119

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
             S +   NLL+   MGE+V+IG+VD+G+WPESE F D G+GPVPS WKG C SG  F S
Sbjct: 120 --SVANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFTS 179

Query: 121 TNCNRKIIGARWYNKGFIV--DVGQEALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQ 180
           + CN+K+IGA+++  GF+   +      + +++S RD  GHGTHVA+ A GS+VP+++Y+
Sbjct: 180 SQCNKKLIGAKYFINGFLATHESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSISYK 239

Query: 181 GLGAGRVRGGAPRARLAIYKAFWSA----AGQGSAADILMAIDQAIHDGVDVLSLSLTVG 240
           GL  G VRGGAPRAR+A+YKA W          S+ADIL A+D+A+HDGVDVLSLS+   
Sbjct: 240 GLAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSIGY- 299

Query: 241 RVPISPEFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRA 300
           R P  PE    +V+A G+FHA  +GI+VVC+GGN GP  QT+ NTAPWI TVAA+T+DR+
Sbjct: 300 RFPYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLDRS 359

Query: 301 FVSSITLGDDTTHLGQSLFTAKNDIGGNLVC------SNSLCS--CEGILRNDT-SLSGN 360
           F + ITLG++   LGQ+++T       +LV       SN   S  CE +  N   +++G 
Sbjct: 360 FPTPITLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMAGK 419

Query: 361 VVLCFSKLANVATLLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATK 420
           VVLCF+      T+  A + V+ A G G+IVA    D L PC     FPC+ VD ++ T 
Sbjct: 420 VVLCFTTSTRYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCED--DFPCVAVDYELGTD 479

Query: 421 IYHYFWDVTNPTVTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNIL 480
           I  Y      P V ++  +  T+VG+P+ T +A FSSRGPNS  PAILKPDIAAPG +IL
Sbjct: 480 ILLYIRSTGLPVVKIQPSK--TLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSIL 539

Query: 481 AAVPPHHRFSDKGFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPS 540
           AA   +  F+D+GF F SGTSMAAP ISG+VALLK++H  WSPAAIRSAI TTA    P 
Sbjct: 540 AATTTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPF 599

Query: 541 GAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISH 600
           G  I AE SP KLADPFDYGGGLVNP  AA PGL+YDL + DYV  Y+C +GY  + IS 
Sbjct: 600 GEQIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYV-LYMCSVGYNETSISQ 659

Query: 601 LTEQKTLCPSERSSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKV 660
           L  + T+C + + SVLD NLPSITIP L++  T+TRT+TNVG L SVY+ VI  P G +V
Sbjct: 660 LVGKGTVCSNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQV 719

Query: 661 NAEPSMLAFNPTVKKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTDLL 703
              P  L FN T K++SFKV +S +   N GY FGSL WSD +H+V IPLSVRT +L
Sbjct: 720 TVTPETLLFNSTTKRVSFKVKVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQIL 768

BLAST of MS012763 vs. ExPASy Swiss-Prot
Match: Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 703.7 bits (1815), Expect = 2.0e-201
Identity = 379/716 (52.93%), Postives = 494/716 (68.99%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKE++  SMV+S+RHGFSGFAAKLT+SQA+K+A++P VV V+P   YK  TTR+WD+LGL
Sbjct: 64  SKEEAHGSMVHSFRHGFSGFAAKLTESQAKKIADLPEVVHVIPDRFYKPATTRTWDYLGL 123

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
             SP+   NLL++  MGE ++IG++D+G+WPESE F D  +GPVPS WKG CESG +FNS
Sbjct: 124 --SPTNPKNLLNQTNMGEQMIIGIIDSGVWPESEVFNDNEIGPVPSHWKGGCESGEDFNS 183

Query: 121 TNCNRKIIGARWYNKGFIV--DVGQEALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQ 180
           ++CN+K+IGA+++   F+   +    + + +++S R + GHGTHVA+ A GS+VPN +Y+
Sbjct: 184 SHCNKKLIGAKYFINAFLATHESFNSSESLDFISPRGYNGHGTHVATIAGGSYVPNTSYK 243

Query: 181 GLGAGRVRGGAPRARLAIYKAFWSA---AGQGSAADILMAIDQAIHDGVDVLSLSLTVGR 240
           GL  G VRGGAPRAR+A+YK  W         S+ADIL A+D+AIHDGVDVLSLSL  G 
Sbjct: 244 GLAGGTVRGGAPRARIAVYKTCWYLDLDIAACSSADILKAMDEAIHDGVDVLSLSL--GF 303

Query: 241 VPISPEFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRAF 300
            P+ PE      +A G+FHA  +GI+VVCA GN GP  QT+ NTAPWI TVAA+T+DR+F
Sbjct: 304 EPLYPETDVRDGIATGAFHAVLKGITVVCAAGNAGPAAQTVGNTAPWILTVAATTLDRSF 363

Query: 301 VSSITLGDDTTHLGQSLFTAKNDIGGNLVC------SNSLCS--CEGILRN-DTSLSGNV 360
           V+ +TLG++   LGQ+++T       +LV       SN   S  CE +L N + +++G V
Sbjct: 364 VTPMTLGNNKVILGQAIYTGTEVGFTSLVYPENPGNSNESFSGTCERLLINSNRTMAGKV 423

Query: 361 VLCFSKLANVATLLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATKI 420
           VLCF++     ++  AA  V+ A G G+I+AGQ  ++L PC     FPC+ VD ++ T I
Sbjct: 424 VLCFTESPYSISVTRAAHYVKRAGGLGVIIAGQPGNVLRPC--LDDFPCVAVDYELGTYI 483

Query: 421 YHYFWDVTNPTVTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNILA 480
             Y     +P V ++  R  T++G+P+ T +A FSSRGPN  S AILKPDIAAPG +ILA
Sbjct: 484 LFYIRSNGSPVVKIQPSR--TLIGQPVGTKVASFSSRGPNPISAAILKPDIAAPGVSILA 543

Query: 481 AVPPHHRFSDKGFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPSG 540
           A   +  F+D+GF F SGTSMA P ISGIVALLK++HP WSPAAIRSAI TTA    P G
Sbjct: 544 ATTTNTTFNDRGFIFLSGTSMATPTISGIVALLKALHPDWSPAAIRSAIVTTAWRTDPFG 603

Query: 541 APILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISHL 600
             I AE SP K ADPFDYGGGLVNP  A  PGL+YDL + DYV  Y+C +GY  + IS L
Sbjct: 604 EQIFAEGSPRKPADPFDYGGGLVNPEKATKPGLVYDLGLEDYV-LYMCSVGYNETSISQL 663

Query: 601 TEQKTLCPSERSSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKVN 660
             + T+C   + SVLD NLPSITIP L+   T+ RT+TNVG L SVYR  +  P GT+V 
Sbjct: 664 VGKGTVCSYPKPSVLDFNLPSITIPNLKEEVTLPRTLTNVGPLESVYRVAVEPPLGTQVT 723

Query: 661 AEPSMLAFNPTVKKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTDLL 703
             P  L FN T K++SFKV++S +   N GY FGSL WSD +H+V IPLSVRT LL
Sbjct: 724 VTPETLVFNSTTKRVSFKVSVSTTHKINTGYYFGSLTWSDSLHNVTIPLSVRTQLL 770

BLAST of MS012763 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 6.5e-200
Identity = 376/718 (52.37%), Postives = 493/718 (68.66%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SK  + ESMVYSYRHGFSGFAAKLT+SQA+KLA+ P VV V+  + Y++ TTR+WD+LGL
Sbjct: 59  SKVDAHESMVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGL 118

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
             S +  +NLL+   MG+ V+IG +DTG+WPESE+F D G+GP+PS WKG CESG +F S
Sbjct: 119 --SVANPNNLLNDTNMGDQVIIGFIDTGVWPESESFNDNGVGPIPSHWKGGCESGEKFIS 178

Query: 121 TNCNRKIIGARWYNKGFIVDVG--QEALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQ 180
           TNCNRK+IGA+++  GF+ +        + +Y+SARDF GHGTH AS A GSFVPN++Y+
Sbjct: 179 TNCNRKLIGAKYFINGFLAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYK 238

Query: 181 GLGAGRVRGGAPRARLAIYKAFWSAAGQG----SAADILMAIDQAIHDGVDVLSLSLTVG 240
           GL  G +RGGAPRAR+AIYKA W     G    S++DIL A+D+++HDGVDVLSLSL   
Sbjct: 239 GLAGGNLRGGAPRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLG-A 298

Query: 241 RVPISPEFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRA 300
           ++P+ PE      +A G+FHA A+GI VVCAGGN GP  QT+LNTAPWI TVAA+T+DR+
Sbjct: 299 QIPLYPETDLRDRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRS 358

Query: 301 FVSSITLGDDTTHLGQSLFTAKNDIG-------GNLVCSNSLCS--CEGI-LRNDTSLSG 360
           F + ITLG+    LGQ+L+T + ++G        N   +N   S  CE + L  + +++G
Sbjct: 359 FPTPITLGNRKVILGQALYTGQ-ELGFTSLVYPENAGFTNETFSGVCERLNLNPNRTMAG 418

Query: 361 NVVLCFSKLANVATLLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVAT 420
            VVLCF+       +  AA+ V+AA G G+I+A      L PC     FPC+ +D ++ T
Sbjct: 419 KVVLCFTTNTLFTAVSRAASYVKAAGGLGVIIARNPGYNLTPCRD--DFPCVAIDYELGT 478

Query: 421 KIYHYFWDVTNPTVTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNI 480
            +  Y     +P V ++  R  T+VG+P+ T +A FSSRGPNS SPAILKPDI APG +I
Sbjct: 479 DVLLYIRSTRSPVVKIQPSR--TLVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSI 538

Query: 481 LAAVPPHHRFSDKGFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGP 540
           LAA  P    S  GF   +GTSMAAP ++G+VALLK++HP WSPAA RSAI TTA    P
Sbjct: 539 LAATSPDSNSSVGGFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVTTAWRTDP 598

Query: 541 SGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDIS 600
            G  I AE S  K+ADPFDYGGG+VNP  AADPGLIYD+   DY+  YLC  GY +S I+
Sbjct: 599 FGEQIFAEGSSRKVADPFDYGGGIVNPEKAADPGLIYDMGPRDYI-LYLCSAGYNDSSIT 658

Query: 601 HLTEQKTLCPSERSSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTK 660
            L    T+C + ++SVLD+NLPSITIP L++  T+TRTVTNVG + SVY+ V+  P G +
Sbjct: 659 QLVGNVTVCSTPKTSVLDVNLPSITIPDLKDEVTLTRTVTNVGTVDSVYKVVVEPPLGIQ 718

Query: 661 VNAEPSMLAFNPTVKKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTDLL 703
           V   P  L FN   K +SF V +S +   N G+ FG+LIW+D +H+V IP+SVRT +L
Sbjct: 719 VVVAPETLVFNSKTKNVSFTVRVSTTHKINTGFYFGNLIWTDSMHNVTIPVSVRTQIL 767

BLAST of MS012763 vs. ExPASy TrEMBL
Match: A0A6J1E3R4 (subtilisin-like protease SBT3.4 OS=Momordica charantia OX=3673 GN=LOC111025797 PE=3 SV=1)

HSP 1 Score: 991.9 bits (2563), Expect = 1.4e-285
Identity = 505/607 (83.20%), Postives = 539/607 (88.80%), Query Frame = 0

Query: 35  MPGVVRVLPSTIYKMQTTRSWDFLGLSSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESE 94
           M GVVRVLP+++YKMQTTRSWDFLGLS+SPSQSSNLLHRGKMGE V+IGV+D+GIWPESE
Sbjct: 1   MAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGEHVIIGVLDSGIWPESE 60

Query: 95  AFGDEGMGPVPSRWKGKCESGAEFNSTNCNRKIIGARWYNKGFIVDVGQEALANEYLSAR 154
           AF DEGMGPVPSRWKGKCESG EFNSTNCNRKIIGARWYNKG I D GQ ALANEY SAR
Sbjct: 61  AFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGXIADXGQXALANEYXSAR 120

Query: 155 DFQGHGTHVASTAAGSFVPNVNYQGLGAGRVRGGAPRARLAIYKAFWSAAGQGSAADILM 214
           DF GHGTH ASTAAGSFVPNVNYQGLGAG VRGGAPRA LAIYK  WS  G GS ADILM
Sbjct: 121 DFXGHGTHXASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLWS-EGAGSGADILM 180

Query: 215 AIDQAIHDGVDVLSLSLTVGRVPISPEFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQ 274
           A+D AIHDGVDVLSLSL  G VPISPEFSEDSVVAIG+FHATARGISVVCAGGNDGP+KQ
Sbjct: 181 AMDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQ 240

Query: 275 TILNTAPWIFTVAASTMDRAFVSSITLGDDTTHLGQSLFTAKNDIGGNLVCS--NSLCSC 334
           TI+NTAPWIFTVAAST+DRAF++ +TLGD+TTHLGQS F  +NDI G LVCS    LCSC
Sbjct: 241 TIVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSC 300

Query: 335 EGILRNDTSLSGNVVLCFSKLANVATLLVAAATVEAANGTGIIVAGQHDDILVPCGQARQ 394
           + IL NDTSLSGN+VLCF+KL ++     A   V  ANG GIIVAGQHDDIL PCG    
Sbjct: 301 KDILGNDTSLSGNIVLCFNKLVDLEMTQKAVVAVRQANGNGIIVAGQHDDILFPCG--AD 360

Query: 395 FPCIVVDPDVATKIYHYFWDVTNPTVTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAI 454
           FPCIV+DPDV TK +HY  D +NP   VR+GRA T+VGKPISTN+AFFSSRGPNSASPAI
Sbjct: 361 FPCIVLDPDVGTKFFHYXLDTSNP--MVRIGRAXTIVGKPISTNVAFFSSRGPNSASPAI 420

Query: 455 LKPDIAAPGSNILAAVPPHHRFSDKGFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIR 514
           LKPDIAAPGSNILAA+ P + FSDKGFAF SGTSMAAPHISGIVALLKS+HPTWSPAAIR
Sbjct: 421 LKPDIAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIR 480

Query: 515 SAISTTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYY 574
           SA+STTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYY
Sbjct: 481 SALSTTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYY 540

Query: 575 LCGMGYKNSDISHLTEQKTLCPSERSSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSV 634
           LCGMGYKNSDIS LTEQKT CPS+RSSVLDLNLPSIT+PALRNSTTVTRTVTNVGN SSV
Sbjct: 541 LCGMGYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSV 600

Query: 635 YRAVIRA 640
           YRAVIRA
Sbjct: 601 YRAVIRA 602

BLAST of MS012763 vs. ExPASy TrEMBL
Match: A0A0A0KWS6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G047950 PE=3 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 7.9e-257
Identity = 458/701 (65.34%), Postives = 551/701 (78.60%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKEKS E+MVYSY+HGFSGFAAKLTKSQAQKL+EM  VVRV+PS++YK+ TTRSWDFLGL
Sbjct: 65  SKEKSMEAMVYSYKHGFSGFAAKLTKSQAQKLSEMSRVVRVVPSSLYKVHTTRSWDFLGL 124

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
           SSSP +SSNLLHR +MGE+V+IGV+DTGIWPESE+F D+G+G +PSRWKG CESG +FNS
Sbjct: 125 SSSPFESSNLLHRAQMGENVIIGVIDTGIWPESESFKDKGVGSIPSRWKGTCESGEQFNS 184

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGL 180
           TNCN+KIIGARW+ KGF+ D+G++ALA EYLS RD  GHGTH AS AAGSFV N+NY   
Sbjct: 185 TNCNKKIIGARWFMKGFVADLGRDALAKEYLSPRDLNGHGTHTASIAAGSFVANINYHNN 244

Query: 181 GAGRVRGGAPRARLAIYKAFWSAAGQGSAADILMAIDQAIHDGVDVLSLSLTVGRVPISP 240
            AG VRGGAP ARLAIYKA W+    GS ADIL AID+AI+DGVDVLS+S+     P  P
Sbjct: 245 AAGTVRGGAPLARLAIYKALWTKDAVGSTADILKAIDEAINDGVDVLSMSIG-SLTPFLP 304

Query: 241 EFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRAFVSSI- 300
           EF+E + +A GSFHA A+GISVVCA GN GP  QT+ N APWIFTVAA+T+DRAF++SI 
Sbjct: 305 EFNEANDIAFGSFHAIAKGISVVCAAGNSGPTPQTVENVAPWIFTVAANTIDRAFLASIT 364

Query: 301 TLGDDTTHLGQSLFTAKNDIGGNLVCSNSLCSCEGILRNDTSLSGNVVLCFSKLANVATL 360
           TL D+TT LGQSL  +K D+   L   ++   C+ +L N+T ++G VV+CFS LA+  T+
Sbjct: 365 TLPDNTTFLGQSLLDSKKDLVAELETLDT-GRCDDLLGNETFINGKVVMCFSNLADHNTI 424

Query: 361 LVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATKIY--HYFWDVTNPT 420
             AA  V  ANGTGIIVAGQ DD L  C  +   PCI+VD DV +K++  +   + TNP 
Sbjct: 425 YDAAMAVARANGTGIIVAGQQDDDLFSCIPS-PIPCILVDTDVGSKLFFINLLQNSTNPV 484

Query: 421 VTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNILAAVPPHHRFSDK 480
           V +R  R  T++GKPI+  I++FSSRGPNS S  ILKPDI+APGSNILAAV PHH F++K
Sbjct: 485 VRLRATR--TIIGKPITPAISYFSSRGPNSVSNPILKPDISAPGSNILAAVSPHHIFNEK 544

Query: 481 GFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPSGAPILAESSPPK 540
           GF   SGTSMA PHIS IVALLKS+HPTWSPAAI+SA+ TTAR +   G PI AE +PPK
Sbjct: 545 GFMLLSGTSMATPHISAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFAEGTPPK 604

Query: 541 LADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISHLTEQKTLCPSER 600
           +ADPFDYGGG+V+ NAA DPGL+YD+   DY+ YYLCGMGYK+ DISHLT++KT+CP +R
Sbjct: 605 MADPFDYGGGIVDANAAVDPGLVYDMGRKDYIDYYLCGMGYKDEDISHLTQRKTVCPLQR 664

Query: 601 SSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKVNAEPSMLAFNPT 660
            SVLDLNLP+ITIP+L NST VTRTVTNVGNLS VY+A I +P+G KV+  P +L FN  
Sbjct: 665 LSVLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAEIESPFGCKVSVNPQVLVFNSQ 724

Query: 661 VKKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVR 699
           VKKISFKV     + RNYGYSFG L W+DG+H VKIPLSVR
Sbjct: 725 VKKISFKVMFFTQVQRNYGYSFGRLTWTDGIHVVKIPLSVR 760

BLAST of MS012763 vs. ExPASy TrEMBL
Match: A0A6J1JBL4 (subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 5.6e-255
Identity = 456/702 (64.96%), Postives = 550/702 (78.35%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKEKS ESMVYSYRHGFSGFAAKLT SQAQKLA MP V RV PS++YKM+TTRSWDFLGL
Sbjct: 63  SKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWDFLGL 122

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
           SSSPS+SSNL HR KMG++V+IGV+DTG WPESE+F D+GMGP+PSRWKG C+SG +FNS
Sbjct: 123 SSSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDFNS 182

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGL 180
           ++CN+K+IGARW+    I D G+EA+  +YLSARD +GHGTH ASTA G+FV NV+Y G 
Sbjct: 183 SHCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVRNVSYFGN 242

Query: 181 GAGRVRGGAPRARLAIYKAFWSAAGQGSAADILMAIDQAIHDGVDVLSLSLTVGRVPISP 240
           G G +RGGAP ARLAIYK  WS +  GS ADIL  ID+AIHDGVDVLS+S+    +P+ P
Sbjct: 243 GRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGKS-IPLFP 302

Query: 241 EFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRAFVSSI- 300
           + ++ + VA+GSFHA A+GISVVCAGGN+G  +QT+ N APW+FTVAAST+DRAF+ SI 
Sbjct: 303 DVNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLVSIT 362

Query: 301 TLGDDTTHLGQSLFTAKNDIGGNLVCSNSLCSCEGILRNDTSLSGNVV-LCFSKLANVAT 360
           TLGD+ T+LGQ     + DI G LV  +  C+  GIL  D  +SGNVV LCF+ LA  A 
Sbjct: 363 TLGDNATYLGQMFL--RKDIVGMLVAMDGRCA--GILGGDIPISGNVVLLCFTDLAKTAP 422

Query: 361 LLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATKIYHYFWDVTNPTV 420
              A    + A   G+I AGQ  D LVPC      PCI VD  V TK++ Y   + +   
Sbjct: 423 ASNAVMPGKQAKVVGVIYAGQQTDNLVPC----DIPCIHVDTHVGTKLFTYL--LNDDDA 482

Query: 421 TVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNILAAVPPHHRFSDKG 480
            +RL    T++GKPIS+ IA+FSSRGPNS SP ILKPDIAAPG+NI+AAVPP+H+  DKG
Sbjct: 483 LIRLRATRTIIGKPISSRIAYFSSRGPNSFSPGILKPDIAAPGANIIAAVPPNHKGGDKG 542

Query: 481 FAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPSGAPILAESSPPKL 540
           FA  SGTSMA PHISGIV L+KS+HPTWSPAAI+SA+ TTARV+ PSG PI AE SPPK+
Sbjct: 543 FATMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSPPKV 602

Query: 541 ADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISHLTEQKTLCPSERS 600
           ADPFDYGGG+V+ NAA DPGLIYDL   DY+ YYLC MGY  +DISHL++QKT+CPS+R+
Sbjct: 603 ADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRA 662

Query: 601 SVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKVNAEPSMLAFNPTV 660
           S+LDLNLP+IT+PAL NSTTVTRTVTNVGNL++VY+AVI+AP G+KV  +P +L FN  V
Sbjct: 663 SILDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNV 722

Query: 661 KKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTD 701
           KKISFKV  S+++ RNYGYSFGSL W+DGVH VK PLSVR D
Sbjct: 723 KKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 753

BLAST of MS012763 vs. ExPASy TrEMBL
Match: A0A6J1EZK4 (subtilisin-like protease SBT3.7 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 1.4e-253
Identity = 457/702 (65.10%), Postives = 547/702 (77.92%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKEKS ESMVYSY+HGFSGFAAKLT SQAQKLA MP V RV PS++YKM TTRSWDFLGL
Sbjct: 65  SKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWDFLGL 124

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
           SSSPS SSNLLHR KMG++V+IGV+D+G WPESE+F D+GMGP+PSRWKG C+ G +FNS
Sbjct: 125 SSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGEDFNS 184

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGL 180
            +CN+K+IGARW+ +  I D G+EA+  +YLSARD +GHGTH ASTA G+FV NV+Y G 
Sbjct: 185 FHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGN 244

Query: 181 GAGRVRGGAPRARLAIYKAFWSAAGQGSAADILMAIDQAIHDGVDVLSLSLTVGRVPISP 240
             G +RGGAP ARLAIYK  WS    GSAADIL  ID+AIHDGVDVLS+S+  G +P+ P
Sbjct: 245 RRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIG-GSIPLPP 304

Query: 241 EFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRAFVSSI- 300
           + SE + VAIGSFHA ARG+SVVCAGGN+G  +QT++N APW+FTVAAST+DRAF++SI 
Sbjct: 305 DVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLTSIT 364

Query: 301 TLGDDTTHLGQSLFTAKNDIGGNLVCSNSLCSCEGILRNDTSLSGNVV-LCFSKLANVAT 360
           TLGD+ T+LGQ+    K D+ G LV  +  C+  G L +D    GNVV LCF  LA  A 
Sbjct: 365 TLGDNATYLGQTFL--KKDVVGKLVVMDRRCA--GHLGSDIPTGGNVVLLCFIDLAMKAA 424

Query: 361 LLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATKIYHYFWDVTNPTV 420
                  V+ A   G+I AGQH DIL PC      PCI VD  V TK+  Y   +++   
Sbjct: 425 ASNPVMPVKQAKVVGVIYAGQHTDILGPC----DVPCIHVDTHVGTKLLTYI--LSDEKA 484

Query: 421 TVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNILAAVPPHHRFSDKG 480
            +RL    T+VGKPIS+ IA+FSSRGPNS SP ILKPDIAAPGSNI+AAVPP+H+  DKG
Sbjct: 485 LIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKG 544

Query: 481 FAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPSGAPILAESSPPKL 540
           FA  SGTSMA PHISGIVAL+KS+ PTWSPAAI+SA+ TTARV+ PSG PI AE SPPK+
Sbjct: 545 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKV 604

Query: 541 ADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISHLTEQKTLCPSERS 600
           ADPFDYGGG+V+ NAA DPGLIYDL   DY+ YY+C MGY  ++ISHL++QK +CPS+R+
Sbjct: 605 ADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRA 664

Query: 601 SVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKVNAEPSMLAFNPTV 660
           SVLDLNLP+IT+PAL NSTTVTRTVTNVGNL++VY+AVI+AP G+KV   P +L FN  V
Sbjct: 665 SVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNV 724

Query: 661 KKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTD 701
           KKISFKV  S+++ RNYGYSFGSL W+DGVH VK PLSVR D
Sbjct: 725 KKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 755

BLAST of MS012763 vs. ExPASy TrEMBL
Match: A0A6J1ETU3 (subtilisin-like protease SBT3.7 isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 1.4e-253
Identity = 457/702 (65.10%), Postives = 547/702 (77.92%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKEKS ESMVYSY+HGFSGFAAKLT SQAQKLA MP V RV PS++YKM TTRSWDFLGL
Sbjct: 63  SKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWDFLGL 122

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
           SSSPS SSNLLHR KMG++V+IGV+D+G WPESE+F D+GMGP+PSRWKG C+ G +FNS
Sbjct: 123 SSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGEDFNS 182

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGL 180
            +CN+K+IGARW+ +  I D G+EA+  +YLSARD +GHGTH ASTA G+FV NV+Y G 
Sbjct: 183 FHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGN 242

Query: 181 GAGRVRGGAPRARLAIYKAFWSAAGQGSAADILMAIDQAIHDGVDVLSLSLTVGRVPISP 240
             G +RGGAP ARLAIYK  WS    GSAADIL  ID+AIHDGVDVLS+S+  G +P+ P
Sbjct: 243 RRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIG-GSIPLPP 302

Query: 241 EFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRAFVSSI- 300
           + SE + VAIGSFHA ARG+SVVCAGGN+G  +QT++N APW+FTVAAST+DRAF++SI 
Sbjct: 303 DVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLTSIT 362

Query: 301 TLGDDTTHLGQSLFTAKNDIGGNLVCSNSLCSCEGILRNDTSLSGNVV-LCFSKLANVAT 360
           TLGD+ T+LGQ+    K D+ G LV  +  C+  G L +D    GNVV LCF  LA  A 
Sbjct: 363 TLGDNATYLGQTFL--KKDVVGKLVVMDRRCA--GHLGSDIPTGGNVVLLCFIDLAMKAA 422

Query: 361 LLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATKIYHYFWDVTNPTV 420
                  V+ A   G+I AGQH DIL PC      PCI VD  V TK+  Y   +++   
Sbjct: 423 ASNPVMPVKQAKVVGVIYAGQHTDILGPC----DVPCIHVDTHVGTKLLTYI--LSDEKA 482

Query: 421 TVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNILAAVPPHHRFSDKG 480
            +RL    T+VGKPIS+ IA+FSSRGPNS SP ILKPDIAAPGSNI+AAVPP+H+  DKG
Sbjct: 483 LIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKG 542

Query: 481 FAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPSGAPILAESSPPKL 540
           FA  SGTSMA PHISGIVAL+KS+ PTWSPAAI+SA+ TTARV+ PSG PI AE SPPK+
Sbjct: 543 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKV 602

Query: 541 ADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISHLTEQKTLCPSERS 600
           ADPFDYGGG+V+ NAA DPGLIYDL   DY+ YY+C MGY  ++ISHL++QK +CPS+R+
Sbjct: 603 ADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRA 662

Query: 601 SVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKVNAEPSMLAFNPTV 660
           SVLDLNLP+IT+PAL NSTTVTRTVTNVGNL++VY+AVI+AP G+KV   P +L FN  V
Sbjct: 663 SVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNV 722

Query: 661 KKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTD 701
           KKISFKV  S+++ RNYGYSFGSL W+DGVH VK PLSVR D
Sbjct: 723 KKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 753

BLAST of MS012763 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 706.4 bits (1822), Expect = 2.2e-203
Identity = 379/717 (52.86%), Postives = 493/717 (68.76%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKE + +SMVYSYRHGFSGFAAKLT+SQA+K+A++P VV V+P + YK+ TTR+WD+LGL
Sbjct: 64  SKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGL 123

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
           S++  +S  LLH   MGE ++IGV+DTG+WPESE F D G GPVPS WKG CE+G  FNS
Sbjct: 124 SAANPKS--LLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNS 183

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALAN--EYLSARDFQGHGTHVASTAAGSFVPNVNYQ 180
           +NCN+K+IGA+++  GF+ +       N  +++S RD  GHGTHV++ A GSFVPN++Y+
Sbjct: 184 SNCNKKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYK 243

Query: 181 GLGAGRVRGGAPRARLAIYKAFWSAAGQG----SAADILMAIDQAIHDGVDVLSLSLTVG 240
           GL  G VRGGAPRA +A+YKA W          S+ADIL A+D+A+HDGVDVLS+SL   
Sbjct: 244 GLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLG-S 303

Query: 241 RVPISPEFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRA 300
            VP+  E      +  G+FHA  +GI+VVC+GGN GP+  T+ NTAPWI TVAA+T+DR+
Sbjct: 304 SVPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRS 363

Query: 301 FVSSITLGDDTTHLGQSLFTAKNDIGGNLVC------SNSLCS--CEGILRN-DTSLSGN 360
           F + +TLG++   LGQ+++T       +LV       SN   S  CE +L N + ++ G 
Sbjct: 364 FATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGK 423

Query: 361 VVLCFSKLANVATLLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATK 420
           VVLCF+       +L AA  V+ A G G+I+A      + PC     FPC+ VD ++ T 
Sbjct: 424 VVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPC--LDDFPCVAVDWELGTD 483

Query: 421 IYHYFWDVTNPTVTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNIL 480
           I  Y     +P V ++  +  T+VG+P+ T +A FSSRGPNS +PAILKPDIAAPG +IL
Sbjct: 484 ILLYTRSSGSPVVKIQPSK--TLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSIL 543

Query: 481 AAVPPHHRFSDKGFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPS 540
           AA   +  FSD+GF   SGTSMAAP ISG+ ALLK++H  WSPAAIRSAI TTA    P 
Sbjct: 544 AAT-TNTTFSDQGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPF 603

Query: 541 GAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISH 600
           G  I AE SPPKLADPFDYGGGLVNP  +A+PGL+YD+ + DYV  Y+C +GY  + IS 
Sbjct: 604 GEQIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQ 663

Query: 601 LTEQKTLCPSERSSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKV 660
           L  + T+C + + SVLD NLPSITIP L++  T+TRTVTNVG L+SVYR  +  P G +V
Sbjct: 664 LIGKTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQV 723

Query: 661 NAEPSMLAFNPTVKKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTDLL 703
              P  L FN T KK+ FKV +S +   N GY FGSL WSD +H+V IPLSVRT +L
Sbjct: 724 TVTPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 771

BLAST of MS012763 vs. TAIR 10
Match: AT4G10550.2 (Subtilase family protein )

HSP 1 Score: 706.4 bits (1822), Expect = 2.2e-203
Identity = 379/717 (52.86%), Postives = 493/717 (68.76%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKE + +SMVYSYRHGFSGFAAKLT+SQA+K+A++P VV V+P + YK+ TTR+WD+LGL
Sbjct: 8   SKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGL 67

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
           S++  +S  LLH   MGE ++IGV+DTG+WPESE F D G GPVPS WKG CE+G  FNS
Sbjct: 68  SAANPKS--LLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNS 127

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALAN--EYLSARDFQGHGTHVASTAAGSFVPNVNYQ 180
           +NCN+K+IGA+++  GF+ +       N  +++S RD  GHGTHV++ A GSFVPN++Y+
Sbjct: 128 SNCNKKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYK 187

Query: 181 GLGAGRVRGGAPRARLAIYKAFWSAAGQG----SAADILMAIDQAIHDGVDVLSLSLTVG 240
           GL  G VRGGAPRA +A+YKA W          S+ADIL A+D+A+HDGVDVLS+SL   
Sbjct: 188 GLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLG-S 247

Query: 241 RVPISPEFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRA 300
            VP+  E      +  G+FHA  +GI+VVC+GGN GP+  T+ NTAPWI TVAA+T+DR+
Sbjct: 248 SVPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRS 307

Query: 301 FVSSITLGDDTTHLGQSLFTAKNDIGGNLVC------SNSLCS--CEGILRN-DTSLSGN 360
           F + +TLG++   LGQ+++T       +LV       SN   S  CE +L N + ++ G 
Sbjct: 308 FATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGK 367

Query: 361 VVLCFSKLANVATLLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATK 420
           VVLCF+       +L AA  V+ A G G+I+A      + PC     FPC+ VD ++ T 
Sbjct: 368 VVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPC--LDDFPCVAVDWELGTD 427

Query: 421 IYHYFWDVTNPTVTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNIL 480
           I  Y     +P V ++  +  T+VG+P+ T +A FSSRGPNS +PAILKPDIAAPG +IL
Sbjct: 428 ILLYTRSSGSPVVKIQPSK--TLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSIL 487

Query: 481 AAVPPHHRFSDKGFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPS 540
           AA   +  FSD+GF   SGTSMAAP ISG+ ALLK++H  WSPAAIRSAI TTA    P 
Sbjct: 488 AAT-TNTTFSDQGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPF 547

Query: 541 GAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISH 600
           G  I AE SPPKLADPFDYGGGLVNP  +A+PGL+YD+ + DYV  Y+C +GY  + IS 
Sbjct: 548 GEQIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQ 607

Query: 601 LTEQKTLCPSERSSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKV 660
           L  + T+C + + SVLD NLPSITIP L++  T+TRTVTNVG L+SVYR  +  P G +V
Sbjct: 608 LIGKTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQV 667

Query: 661 NAEPSMLAFNPTVKKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTDLL 703
              P  L FN T KK+ FKV +S +   N GY FGSL WSD +H+V IPLSVRT +L
Sbjct: 668 TVTPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 715

BLAST of MS012763 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 706.4 bits (1822), Expect = 2.2e-203
Identity = 379/717 (52.86%), Postives = 493/717 (68.76%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKE + +SMVYSYRHGFSGFAAKLT+SQA+K+A++P VV V+P + YK+ TTR+WD+LGL
Sbjct: 80  SKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGL 139

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
           S++  +S  LLH   MGE ++IGV+DTG+WPESE F D G GPVPS WKG CE+G  FNS
Sbjct: 140 SAANPKS--LLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNS 199

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALAN--EYLSARDFQGHGTHVASTAAGSFVPNVNYQ 180
           +NCN+K+IGA+++  GF+ +       N  +++S RD  GHGTHV++ A GSFVPN++Y+
Sbjct: 200 SNCNKKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYK 259

Query: 181 GLGAGRVRGGAPRARLAIYKAFWSAAGQG----SAADILMAIDQAIHDGVDVLSLSLTVG 240
           GL  G VRGGAPRA +A+YKA W          S+ADIL A+D+A+HDGVDVLS+SL   
Sbjct: 260 GLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLG-S 319

Query: 241 RVPISPEFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRA 300
            VP+  E      +  G+FHA  +GI+VVC+GGN GP+  T+ NTAPWI TVAA+T+DR+
Sbjct: 320 SVPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRS 379

Query: 301 FVSSITLGDDTTHLGQSLFTAKNDIGGNLVC------SNSLCS--CEGILRN-DTSLSGN 360
           F + +TLG++   LGQ+++T       +LV       SN   S  CE +L N + ++ G 
Sbjct: 380 FATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGK 439

Query: 361 VVLCFSKLANVATLLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATK 420
           VVLCF+       +L AA  V+ A G G+I+A      + PC     FPC+ VD ++ T 
Sbjct: 440 VVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPC--LDDFPCVAVDWELGTD 499

Query: 421 IYHYFWDVTNPTVTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNIL 480
           I  Y     +P V ++  +  T+VG+P+ T +A FSSRGPNS +PAILKPDIAAPG +IL
Sbjct: 500 ILLYTRSSGSPVVKIQPSK--TLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSIL 559

Query: 481 AAVPPHHRFSDKGFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPS 540
           AA   +  FSD+GF   SGTSMAAP ISG+ ALLK++H  WSPAAIRSAI TTA    P 
Sbjct: 560 AAT-TNTTFSDQGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPF 619

Query: 541 GAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISH 600
           G  I AE SPPKLADPFDYGGGLVNP  +A+PGL+YD+ + DYV  Y+C +GY  + IS 
Sbjct: 620 GEQIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQ 679

Query: 601 LTEQKTLCPSERSSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKV 660
           L  + T+C + + SVLD NLPSITIP L++  T+TRTVTNVG L+SVYR  +  P G +V
Sbjct: 680 LIGKTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQV 739

Query: 661 NAEPSMLAFNPTVKKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTDLL 703
              P  L FN T KK+ FKV +S +   N GY FGSL WSD +H+V IPLSVRT +L
Sbjct: 740 TVTPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 787

BLAST of MS012763 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 705.7 bits (1820), Expect = 3.8e-203
Identity = 378/717 (52.72%), Postives = 488/717 (68.06%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SK+ + ESMVYSYRHGFSGFAAKLTKSQA+K+A+ P V+ V+P + Y++ TTR WD+LG 
Sbjct: 59  SKDDAHESMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYELATTRIWDYLG- 118

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
             S   S NL+    MG+  +IGV+DTG+WPESE+F D G+GPVPS WKG CE G  F S
Sbjct: 119 -PSADNSKNLVSDTNMGDQTIIGVIDTGVWPESESFNDYGVGPVPSHWKGGCEPGENFIS 178

Query: 121 TNCNRKIIGARWYNKGFIVDVGQEALAN-EYLSARDFQGHGTHVASTAAGSFVPNVNYQG 180
           TNCNRK+IGA+++  GF+ +    A  + +Y+SARDF GHGTHVAS A GSFVPNV+Y+G
Sbjct: 179 TNCNRKLIGAKYFINGFLAENQFNATESPDYISARDFDGHGTHVASIAGGSFVPNVSYKG 238

Query: 181 LGAGRVRGGAPRARLAIYKAFWSA----AGQGSAADILMAIDQAIHDGVDVLSLSLTVGR 240
           LG G +RGGAPRAR+A+YKA W          S +DI+ AID+AIHDGVDVLS+SL  GR
Sbjct: 239 LGRGTLRGGAPRARIAMYKACWYINELDGVTCSFSDIMKAIDEAIHDGVDVLSISLG-GR 298

Query: 241 VPISPEFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRAF 300
           VP++ E      +A G+FHA A+GI VVCAGGN GP+ QT++NTAPWI TVAA+T+DR+F
Sbjct: 299 VPLNSETDLRDGIATGAFHAVAKGIVVVCAGGNAGPSSQTVVNTAPWILTVAATTLDRSF 358

Query: 301 VSSITLGDDTTHLGQSLFTA----------KNDIGGNLVCSNSLCSCEGILRNDTSLSGN 360
            + I LG++   LGQ+++              D G ++   + +C    +  N T ++G 
Sbjct: 359 ATPIILGNNQVILGQAMYIGPELGFTSLVYPEDPGNSIDTFSGVCESLNLNSNRT-MAGK 418

Query: 361 VVLCFSKLANVATLLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATK 420
           VVLCF+   +   +  AA+ V+AA G G+I+A      L PC     FPC+ +D ++ T 
Sbjct: 419 VVLCFTTARDFTVVSTAASIVKAAGGLGLIIARNPGYNLAPCSD--DFPCVAIDNELGTD 478

Query: 421 IYHYFWDVTNPTVTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNIL 480
           I  Y     +P V ++  R  T+VG+P+ T +A FSSRGPNS SPAILKPDIAAPG +IL
Sbjct: 479 ILFYIRYTGSPVVKIQPSR--TLVGEPVGTKVATFSSRGPNSISPAILKPDIAAPGVSIL 538

Query: 481 AAVPPHHRFSDKGFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPS 540
           AA  P+   +  GF   SGTSMAAP ISG++ALLKS+HP WSPAA RSAI TTA    P 
Sbjct: 539 AATSPNDTLNAGGFVMRSGTSMAAPVISGVIALLKSLHPDWSPAAFRSAIVTTAWRTDPF 598

Query: 541 GAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISH 600
           G  I AESS  K+ DPFDYGGGLVNP  AA+PGLI D+   DYV  YLC  GY +S IS 
Sbjct: 599 GEQIAAESSSLKVPDPFDYGGGLVNPEKAAEPGLILDMDSQDYV-LYLCSAGYNDSSISR 658

Query: 601 LTEQKTLCPSERSSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKV 660
           L  + T+C + + SVLD+NLPSITIP L++  T+TRTVTNVG + SVY+ ++  P G +V
Sbjct: 659 LVGKVTVCSNPKPSVLDINLPSITIPNLKDEVTLTRTVTNVGPVDSVYKVLVEPPLGIQV 718

Query: 661 NAEPSMLAFNPTVKKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTDLL 703
              P  L FN   K +SF V +S +   N G+ FGSL W+D +H+V IP+SVRT +L
Sbjct: 719 VVTPETLVFNSKTKSVSFTVIVSTTHKINTGFYFGSLTWTDSIHNVVIPVSVRTQIL 766

BLAST of MS012763 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 704.5 bits (1817), Expect = 8.4e-203
Identity = 383/717 (53.42%), Postives = 491/717 (68.48%), Query Frame = 0

Query: 1   SKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLPSTIYKMQTTRSWDFLGL 60
           SKE +  SMV+SYRHGFSGFAAKLTKSQA+KLA++P VV V P + Y++ TTR+WD+LGL
Sbjct: 60  SKEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDYLGL 119

Query: 61  SSSPSQSSNLLHRGKMGEDVVIGVVDTGIWPESEAFGDEGMGPVPSRWKGKCESGAEFNS 120
             S +   NLL+   MGE+V+IG+VD+G+WPESE F D G+GPVPS WKG C SG  F S
Sbjct: 120 --SVANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFTS 179

Query: 121 TNCNRKIIGARWYNKGFIV--DVGQEALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQ 180
           + CN+K+IGA+++  GF+   +      + +++S RD  GHGTHVA+ A GS+VP+++Y+
Sbjct: 180 SQCNKKLIGAKYFINGFLATHESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSISYK 239

Query: 181 GLGAGRVRGGAPRARLAIYKAFWSA----AGQGSAADILMAIDQAIHDGVDVLSLSLTVG 240
           GL  G VRGGAPRAR+A+YKA W          S+ADIL A+D+A+HDGVDVLSLS+   
Sbjct: 240 GLAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSIGY- 299

Query: 241 RVPISPEFSEDSVVAIGSFHATARGISVVCAGGNDGPNKQTILNTAPWIFTVAASTMDRA 300
           R P  PE    +V+A G+FHA  +GI+VVC+GGN GP  QT+ NTAPWI TVAA+T+DR+
Sbjct: 300 RFPYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLDRS 359

Query: 301 FVSSITLGDDTTHLGQSLFTAKNDIGGNLVC------SNSLCS--CEGILRNDT-SLSGN 360
           F + ITLG++   LGQ+++T       +LV       SN   S  CE +  N   +++G 
Sbjct: 360 FPTPITLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMAGK 419

Query: 361 VVLCFSKLANVATLLVAAATVEAANGTGIIVAGQHDDILVPCGQARQFPCIVVDPDVATK 420
           VVLCF+      T+  A + V+ A G G+IVA    D L PC     FPC+ VD ++ T 
Sbjct: 420 VVLCFTTSTRYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCED--DFPCVAVDYELGTD 479

Query: 421 IYHYFWDVTNPTVTVRLGRATTVVGKPISTNIAFFSSRGPNSASPAILKPDIAAPGSNIL 480
           I  Y      P V ++  +  T+VG+P+ T +A FSSRGPNS  PAILKPDIAAPG +IL
Sbjct: 480 ILLYIRSTGLPVVKIQPSK--TLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSIL 539

Query: 481 AAVPPHHRFSDKGFAFNSGTSMAAPHISGIVALLKSMHPTWSPAAIRSAISTTARVKGPS 540
           AA   +  F+D+GF F SGTSMAAP ISG+VALLK++H  WSPAAIRSAI TTA    P 
Sbjct: 540 AATTTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPF 599

Query: 541 GAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISH 600
           G  I AE SP KLADPFDYGGGLVNP  AA PGL+YDL + DYV  Y+C +GY  + IS 
Sbjct: 600 GEQIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYV-LYMCSVGYNETSISQ 659

Query: 601 LTEQKTLCPSERSSVLDLNLPSITIPALRNSTTVTRTVTNVGNLSSVYRAVIRAPYGTKV 660
           L  + T+C + + SVLD NLPSITIP L++  T+TRT+TNVG L SVY+ VI  P G +V
Sbjct: 660 LVGKGTVCSNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQV 719

Query: 661 NAEPSMLAFNPTVKKISFKVTISASIHRNYGYSFGSLIWSDGVHHVKIPLSVRTDLL 703
              P  L FN T K++SFKV +S +   N GY FGSL WSD +H+V IPLSVRT +L
Sbjct: 720 TVTPETLLFNSTTKRVSFKVKVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQIL 768