MS012739 (gene) Bitter gourd (TR) v1

BLAST of MS012739 vs. NCBI nr
Match: XP_022136857.1 (secretion-regulating guanine nucleotide exchange factor [Momordica charantia])

HSP 1 Score: 897.5 bits (2318), Expect = 4.6e-257
Identity = 442/443 (99.77%), Postives = 442/443 (99.77%), Query Frame = 0

Query: 1   MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT 60
           MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT
Sbjct: 1   MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT 60

Query: 61  VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL 120
           VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL
Sbjct: 61  VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL 120

Query: 121 VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS 180
           VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS
Sbjct: 121 VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS 180

Query: 181 FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL 240
           FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL
Sbjct: 181 FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL 240

Query: 241 VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300
           VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR
Sbjct: 241 VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300

Query: 301 ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTSQLGREGP 360
           ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQT QLGREGP
Sbjct: 301 ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTPQLGREGP 360

Query: 361 NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD 420
           NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD
Sbjct: 361 NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD 420

Query: 421 SLENCNVVQAVSGFDHNLVLVAD 444
           SLENCNVVQAVSGFDHNLVLVAD
Sbjct: 421 SLENCNVVQAVSGFDHNLVLVAD 443

BLAST of MS012739 vs. NCBI nr
Match: XP_022984599.1 (ultraviolet-B receptor UVR8 [Cucurbita maxima])

HSP 1 Score: 805.4 bits (2079), Expect = 2.4e-229
Identity = 392/443 (88.49%), Postives = 414/443 (93.45%), Query Frame = 0

Query: 1   MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT 60
           MIRGIRILA  Q RA+SI+P+GRRC+ S AVMSFGDGSHGAIGLPTS+TGIGGAAYEP T
Sbjct: 1   MIRGIRILAGEQRRALSIIPFGRRCLSSRAVMSFGDGSHGAIGLPTSLTGIGGAAYEPTT 60

Query: 61  VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL 120
           VPGLPPDVTSVAAGHYHSLA+DSQG LWAWGRD EAQLGRGFASPRDSWNVP RVIGLDL
Sbjct: 61  VPGLPPDVTSVAAGHYHSLAIDSQGCLWAWGRDQEAQLGRGFASPRDSWNVPNRVIGLDL 120

Query: 121 VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS 180
           VNVRSASASGVVSAA+GDDGSLWIWGKSKRGQLGLGKDV EAV PSRVQA+EGE+IVKVS
Sbjct: 121 VNVRSASASGVVSAALGDDGSLWIWGKSKRGQLGLGKDVTEAVLPSRVQAVEGERIVKVS 180

Query: 181 FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL 240
           FGWGHALA SEDGKLFGWGYYADGRLGN+GRTLKMSPLDSSS  STNGQ+   I+LAERL
Sbjct: 181 FGWGHALALSEDGKLFGWGYYADGRLGNIGRTLKMSPLDSSSYRSTNGQEQPNIQLAERL 240

Query: 241 VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300
           VLESM+DEKNMPIIWEPH VEDLTG+EVVDIACGLDHSLVLCRDGTLLSCGSN+YGQLGR
Sbjct: 241 VLESMEDEKNMPIIWEPHLVEDLTGIEVVDIACGLDHSLVLCRDGTLLSCGSNVYGQLGR 300

Query: 301 ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTSQLGREGP 360
            RQDLGLLPVD+SFHVKSVAAGLGHSLAL QDTSN+++ D  SVISWGWNQTSQLGREG 
Sbjct: 301 VRQDLGLLPVDSSFHVKSVAAGLGHSLALYQDTSNKDVSDATSVISWGWNQTSQLGREGQ 360

Query: 361 NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD 420
           NSIPL IE LAGEIP+SVSGGRAHSI L SRGEVWVWGCG+DGRLGLGSSSDESEP+FLD
Sbjct: 361 NSIPLIIEGLAGEIPISVSGGRAHSIALSSRGEVWVWGCGRDGRLGLGSSSDESEPVFLD 420

Query: 421 SLENCNVVQAVSGFDHNLVLVAD 444
           SLENCNVVQAVSGFDHNLVLV D
Sbjct: 421 SLENCNVVQAVSGFDHNLVLVVD 443

BLAST of MS012739 vs. NCBI nr
Match: XP_022929402.1 (ultraviolet-B receptor UVR8 [Cucurbita moschata])

HSP 1 Score: 804.7 bits (2077), Expect = 4.1e-229
Identity = 391/443 (88.26%), Postives = 416/443 (93.91%), Query Frame = 0

Query: 1   MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT 60
           MIRGIRILA  Q RA+SI+P+GRRC+ S AVMSFGDGSHGAIGLPTS+TGIGGAAYEP T
Sbjct: 1   MIRGIRILAGEQRRALSIIPFGRRCLSSRAVMSFGDGSHGAIGLPTSLTGIGGAAYEPTT 60

Query: 61  VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL 120
           VP LPPDVTSVAAGHYHSLA+DSQG LWAWGRD EAQLGRGFASPRDSWNVP RVIGLDL
Sbjct: 61  VPVLPPDVTSVAAGHYHSLAIDSQGCLWAWGRDQEAQLGRGFASPRDSWNVPNRVIGLDL 120

Query: 121 VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS 180
           VNVRSASASGVVSAA+GDDGSLWIWG+SKRGQLGLGKDV EAV PSRVQA+EGE+IVKVS
Sbjct: 121 VNVRSASASGVVSAALGDDGSLWIWGRSKRGQLGLGKDVTEAVIPSRVQAVEGERIVKVS 180

Query: 181 FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL 240
           FGWGHALA SEDGKLFGWGYYADGRLGN+G+TLKMSPLDSSS  STNGQ+   I+LAERL
Sbjct: 181 FGWGHALALSEDGKLFGWGYYADGRLGNIGKTLKMSPLDSSSYRSTNGQEQPNIQLAERL 240

Query: 241 VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300
           VLESM+DEKNMPIIWEPH VEDLTG+EVVDIACGLDHSLVLCRDGTLLSCGSN+YGQLGR
Sbjct: 241 VLESMEDEKNMPIIWEPHLVEDLTGIEVVDIACGLDHSLVLCRDGTLLSCGSNVYGQLGR 300

Query: 301 ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTSQLGREGP 360
            RQDLGLLPVD+SFHVKSVAAGLGHSLAL QDTSN+EI DV S+ISWGWNQTSQLGREGP
Sbjct: 301 VRQDLGLLPVDSSFHVKSVAAGLGHSLALYQDTSNKEISDVTSIISWGWNQTSQLGREGP 360

Query: 361 NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD 420
           N+IPLAIE LAGEIP+SVSGGRAHSI L SRGEVWVWGCG+DGRLGLGSSSDESEP+FLD
Sbjct: 361 NNIPLAIEGLAGEIPISVSGGRAHSIALSSRGEVWVWGCGRDGRLGLGSSSDESEPVFLD 420

Query: 421 SLENCNVVQAVSGFDHNLVLVAD 444
           SLENCNV+QAVSGFDHNLVLV D
Sbjct: 421 SLENCNVMQAVSGFDHNLVLVVD 443

BLAST of MS012739 vs. NCBI nr
Match: XP_038905737.1 (ultraviolet-B receptor UVR8 [Benincasa hispida])

HSP 1 Score: 802.4 bits (2071), Expect = 2.0e-228
Identity = 395/443 (89.16%), Postives = 413/443 (93.23%), Query Frame = 0

Query: 1   MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT 60
           MIRGIRILA+   RAVSI+P+ RRCI S AVMSFGDGSHGAIGLPTS+TGIGGAAYEP T
Sbjct: 2   MIRGIRILAKEHQRAVSIIPFRRRCISSRAVMSFGDGSHGAIGLPTSLTGIGGAAYEPTT 61

Query: 61  VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL 120
           VPGLPPDVTS+AAGHYHSLAVDSQG LWAWGRDHEAQLGRGFASPRDSWNVP RVIGLDL
Sbjct: 62  VPGLPPDVTSIAAGHYHSLAVDSQGCLWAWGRDHEAQLGRGFASPRDSWNVPNRVIGLDL 121

Query: 121 VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS 180
           VN+ SASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDV EAV PSRVQALEGEKIVKVS
Sbjct: 122 VNISSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVTEAVIPSRVQALEGEKIVKVS 181

Query: 181 FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL 240
           FGWGH LA SE+GKLFGWGYYADGRLGN+GRTLKMSPLDSSS +STNGQ+   IELAERL
Sbjct: 182 FGWGHVLAFSENGKLFGWGYYADGRLGNIGRTLKMSPLDSSSYSSTNGQEQPNIELAERL 241

Query: 241 VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300
           VLESM+DEKNMPIIWEP  VEDLT VEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR
Sbjct: 242 VLESMEDEKNMPIIWEPCLVEDLTSVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 301

Query: 301 ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTSQLGREGP 360
           ARQDLGLLPVD+SFHVKSVAAGLGHSLALCQDTSN++I D  SVISWGWNQTSQLGREGP
Sbjct: 302 ARQDLGLLPVDSSFHVKSVAAGLGHSLALCQDTSNKDISDATSVISWGWNQTSQLGREGP 361

Query: 361 NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD 420
           N+IPLAIE LAGE P+ VSGGRAHSI LGSRGEVWVWGCGKDGRLGLGSSSDESEP+FLD
Sbjct: 362 NNIPLAIEGLAGETPILVSGGRAHSIALGSRGEVWVWGCGKDGRLGLGSSSDESEPVFLD 421

Query: 421 SLENCNVVQAVSGFDHNLVLVAD 444
           SLE+ NVVQAVSGFDHNLVLV D
Sbjct: 422 SLEHRNVVQAVSGFDHNLVLVVD 444

BLAST of MS012739 vs. NCBI nr
Match: KAG6577213.1 (Ultraviolet-B receptor UVR8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 802.0 bits (2070), Expect = 2.6e-228
Identity = 389/443 (87.81%), Postives = 415/443 (93.68%), Query Frame = 0

Query: 1   MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT 60
           MIRGIRILA  Q RA+SI+P+GRRC+ S AVMSFGDGSHGAIGLPTS+TGIGGAAYEP T
Sbjct: 1   MIRGIRILAGEQRRALSIIPFGRRCLSSRAVMSFGDGSHGAIGLPTSLTGIGGAAYEPTT 60

Query: 61  VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL 120
           VP LPPDVTSVAAGHYHSLA+DSQG LWAWGRD EAQLGRGFASPRDSWNVP RVIGLDL
Sbjct: 61  VPVLPPDVTSVAAGHYHSLAIDSQGCLWAWGRDQEAQLGRGFASPRDSWNVPNRVIGLDL 120

Query: 121 VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS 180
           VNVRSASASGVVSAA+GDDGSLWIWG+SKRGQLGLGKDV EAV PSRVQA+EGE+IVKVS
Sbjct: 121 VNVRSASASGVVSAALGDDGSLWIWGRSKRGQLGLGKDVTEAVIPSRVQAVEGERIVKVS 180

Query: 181 FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL 240
           FGWGHALA SEDGKLFGWGYYADGRLGN+G+TLKMSPLDSSS  STNGQ+   I+LAERL
Sbjct: 181 FGWGHALALSEDGKLFGWGYYADGRLGNIGKTLKMSPLDSSSYRSTNGQEQPNIQLAERL 240

Query: 241 VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300
           VLESM+DEKNMPIIWEPH VEDLTG+EVVDIACGLDHSLVLCRDGTLLSCGSN+YGQLGR
Sbjct: 241 VLESMEDEKNMPIIWEPHLVEDLTGIEVVDIACGLDHSLVLCRDGTLLSCGSNVYGQLGR 300

Query: 301 ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTSQLGREGP 360
            RQDLGLLPVD+SFHVKSVAAGLGHSLAL QDTSN++I D  S+ISWGWNQTSQLGREGP
Sbjct: 301 VRQDLGLLPVDSSFHVKSVAAGLGHSLALYQDTSNKDISDATSIISWGWNQTSQLGREGP 360

Query: 361 NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD 420
           N+IPLAIE LAGEIP++VSGGRAHSI L SRGEVWVWGCG+DGRLGLGSSSDESEP+FLD
Sbjct: 361 NNIPLAIEGLAGEIPIAVSGGRAHSIALSSRGEVWVWGCGRDGRLGLGSSSDESEPVFLD 420

Query: 421 SLENCNVVQAVSGFDHNLVLVAD 444
           SLENCNVVQAVSGFDHNLVLV D
Sbjct: 421 SLENCNVVQAVSGFDHNLVLVVD 443

BLAST of MS012739 vs. ExPASy Swiss-Prot
Match: Q9FN03 (Ultraviolet-B receptor UVR8 OS=Arabidopsis thaliana OX=3702 GN=UVR8 PE=1 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 3.3e-32
Identity = 113/436 (25.92%), Postives = 189/436 (43.35%), Query Frame = 0

Query: 3   RGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIG--------LPTSITGIGGA 62
           R + I++ G S +V++       +    V S+G G  G +G         PT ++ + G 
Sbjct: 15  RKVLIISAGASHSVAL-------LSGDIVCSWGRGEDGQLGHGDAEDRPSPTQLSALDG- 74

Query: 63  AYEPITVPGLPPDVTSVAAGHYHSLAVDSQG-HLWAWGRDHEAQLGRGFASPRDSWNVPK 122
                        + SV  G  H++A    G  +++WG     +LG G +S       P 
Sbjct: 75  -----------HQIVSVTCGADHTVAYSQSGMEVYSWGWGDFGRLGHGNSS---DLFTPL 134

Query: 123 RVIGLDLVNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEG 182
            +  L  + ++  +       A+  +G +  WG+++ GQLGLG D  +++ P ++QA EG
Sbjct: 135 PIKALHGIRIKQIACGDSHCLAVTMEGEVQSWGRNQNGQLGLG-DTEDSLVPQKIQAFEG 194

Query: 183 EKIVKVSFGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQK 242
            +I  V+ G  H  A +EDG L+GWG+   GR GN+G   +   L     TST G+K+  
Sbjct: 195 IRIKMVAAGAEHTAAVTEDGDLYGWGW---GRYGNLGLGDRTDRLVPERVTSTGGEKMSM 254

Query: 243 IELAERLVLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSN 302
                                                +ACG  H++ +   G L + G +
Sbjct: 255 -------------------------------------VACGWRHTISVSYSGALYTYGWS 314

Query: 303 IYGQLGRARQDLGLLP-----VDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWG 362
            YGQLG    +  L+P     +  SF +  ++ G  H++AL  D           +  WG
Sbjct: 315 KYGQLGHGDLEDHLIPHKLEALSNSF-ISQISGGWRHTMALTSDG---------KLYGWG 374

Query: 363 WNQTSQ--LGREGPNSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLG 422
           WN+  Q  +G       P+ +     +  V VS G  H++ +  R  V+ WG G +G+LG
Sbjct: 375 WNKFGQVGVGNNLDQCSPVQVRFPDDQKVVQVSCGWRHTLAVTERNNVFAWGRGTNGQLG 377

BLAST of MS012739 vs. ExPASy Swiss-Prot
Match: Q15751 (Probable E3 ubiquitin-protein ligase HERC1 OS=Homo sapiens OX=9606 GN=HERC1 PE=1 SV=2)

HSP 1 Score: 133.3 bits (334), Expect = 6.9e-30
Identity = 113/422 (26.78%), Postives = 185/422 (43.84%), Query Frame = 0

Query: 31   VMSFGDGSHGAIGLPTSITGIGGAAYEPITVPGLPPDVT---SVAAGHYHSLAVDSQGHL 90
            V  +G G HG +            A   + VP   P  +    V  G   +  + + G +
Sbjct: 3999 VYLWGAGRHGQL----------AEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTV 4058

Query: 91   WAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDLVNVRSASASGVVSAAIGDDGSLWIWGK 150
             A G     +LG+G +       V   + G  +  + ++  S   S A+ + G ++ WG 
Sbjct: 4059 LACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHSMALTESGEVFSWGD 4118

Query: 151  SKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVSFGWGHALACSEDGKLFGWGYYADGRLG 210
               G+LG G    +  RP +++AL+GE++V++S G+ H+   + DGKLF +G    GRLG
Sbjct: 4119 GDYGKLGHGNSDRQR-RPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLG 4178

Query: 211  NVGRTLKMSPLDSSSCTSTNGQKLQKIELAERLVLESMKDEKNMPIIWEPHFVEDLTGVE 270
                             +T+ +KL                         P  V  L G +
Sbjct: 4179 ---------------LGNTSNKKL-------------------------PERVTALEGYQ 4238

Query: 271  VVDIACGLDHSLVLCRDGTLL-SCGSNIYGQLG----RARQDLGLLPVDTSFHVKSVAAG 330
            +  +ACGL+H+L +  DG+++ + G   YG+LG     A+     + V     +K VA G
Sbjct: 4239 IGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKIDVLCGIGIKKVACG 4298

Query: 331  LGHSLALCQDTSNREIRDVVSVISWGWNQTSQL--GREGPNSIPLAIEELAGEIPVSVSG 390
               S+AL +D           V ++G ++   L  GR   ++ P  I  LAG I   V+ 
Sbjct: 4299 TQFSVALTKDG---------HVYTFGQDRLIGLPEGRARNHNRPQQIPVLAGVIIEDVAV 4358

Query: 391  GRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLDSLENCNVVQAVSGFDHNLVL 443
            G  H++ L S G+V+ WG   +G+LGLG ++   EP  +  L+  NV Q  +G  H+   
Sbjct: 4359 GAEHTLALASNGDVYAWGSNSEGQLGLGHTNHVREPTLVTGLQGKNVRQISAGRCHSAAW 4360

BLAST of MS012739 vs. ExPASy Swiss-Prot
Match: Q9VR91 (Probable E3 ubiquitin-protein ligase HERC2 OS=Drosophila melanogaster OX=7227 GN=HERC2 PE=1 SV=3)

HSP 1 Score: 122.1 bits (305), Expect = 1.6e-26
Identity = 100/376 (26.60%), Postives = 160/376 (42.55%), Query Frame = 0

Query: 71   VAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDLVNVR--SASA 130
            +A G     AV   G L+A G     +LG G +   DSW +P  +  L  V V+  + ++
Sbjct: 4087 LAGGEQSLFAVTPDGKLFATGYGSGGRLGVGGS---DSWAIPTLLGSLQHVFVKKVAVNS 4146

Query: 131  SGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVSFGWGHALA 190
             G    A+  +G ++ WG+ + G+LG G + M   RP  V+ L G  +  ++ G  H+ A
Sbjct: 4147 GGKHCLALTTEGEVYAWGEGEDGKLGHG-NRMSYDRPKLVEHLNGMSVADIACGSAHSAA 4206

Query: 191  CSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERLVLESMKDE 250
             +  G +  WG    GRLG                   +G    ++              
Sbjct: 4207 ITASGHVLTWGKGRYGRLG-------------------HGDSEDQL-------------- 4266

Query: 251  KNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLC--RDGTLLSCGSNIYGQLGRARQDLG 310
                    P  VE L G   +DIACG   +  LC   D  + S G   YG+LGR   D  
Sbjct: 4267 -------RPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGC 4326

Query: 311  LLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTSQLGREGPNSI--P 370
             LP    + ++S+ AGLG     C    +  +    +V +WG     +LG    + +  P
Sbjct: 4327 KLP----YKIESL-AGLGVVKVECGSQFSVALTKSGAVYTWGKGDFHRLGHGSVDHVRRP 4386

Query: 371  LAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLDSLEN 430
              +  L G+  +S++ G  H +     GEV+ WG   +G+LG G+ +    P  + +L+ 
Sbjct: 4387 KKVAALQGKKIISIATGSLHCVACSDSGEVYTWGDNDEGQLGDGTVTAIQRPRLVAALQG 4413

Query: 431  CNVVQAVSGFDHNLVL 441
             ++V+   G  H L L
Sbjct: 4447 KHIVKVTCGSAHTLAL 4413

BLAST of MS012739 vs. ExPASy Swiss-Prot
Match: Q91ZR4 (Serine/threonine-protein kinase Nek8 OS=Mus musculus OX=10090 GN=Nek8 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.1e-26
Identity = 107/406 (26.35%), Postives = 167/406 (41.13%), Query Frame = 0

Query: 46  TSITGIGGAAYEPITVPGLPPDVTSVAAGHYHSLAVDSQGHLWAW-----GRDHEAQLGR 105
           +S+   GG    P+ +P L  +V  VAAG      V   G L  W     G      L  
Sbjct: 318 SSVYAWGGGLSSPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGTLLPG 377

Query: 106 GFASPRDSWNVPKRVIGLDLVNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVM 165
               P+  + V + + G   V ++  +   + +A + D G +  +G    G LG G ++ 
Sbjct: 378 AVELPQPQF-VSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHG-NLT 437

Query: 166 EAVRPSRVQALEGEKIVKVSFGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDS 225
           +  +P+ V+AL G ++V+V+ G  H LA S DG+LF WG    GRLG       +   +S
Sbjct: 438 DISQPTIVEALLGYEMVQVACGASHVLALSTDGELFAWGRGDGGRLG-------LGTRES 497

Query: 226 SSCTSTNGQKLQKIELAERLVLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLV 285
            +C                                 P  V    G E   + CG+D S++
Sbjct: 498 HNC---------------------------------PQQVPVAPGQEAQRVVCGIDSSMI 557

Query: 286 LCRDGTLLSCGSNIYGQLGRARQDLGLLPVDTSFHVKSV------AAGLGHSLALCQDTS 345
           L   G +L+CGSN + +LG     L   PV      +++      +A L     LC D  
Sbjct: 558 LTSPGRVLACGSNRFNKLGLDHLSLDEEPVPYQQVEEALSFTPLGSAPLDQEPLLCVDLG 617

Query: 346 NREIRDVVS---VISWGWNQTSQLGREG--PNSIPLAIEELAGEIPVSVSGGRAHSIVLG 405
                 + +     ++G NQ  QLG      +  P  ++ L G   V V+ G A ++ +G
Sbjct: 618 TAHSAAITASGDCYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMVACGDAFTVAVG 677

Query: 406 SRGEVWVWGCGKDGRLGLGSSS---------DESEPIFLDSLENCN 427
           + GEV+ WG G  GRLG              DE+ P  + S+  C+
Sbjct: 678 AEGEVYSWGKGTRGRLGRRDEDAGLPRPVQLDETHPYMVTSVSCCH 681

BLAST of MS012739 vs. ExPASy Swiss-Prot
Match: O95714 (E3 ubiquitin-protein ligase HERC2 OS=Homo sapiens OX=9606 GN=HERC2 PE=1 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 2.7e-26
Identity = 103/376 (27.39%), Postives = 151/376 (40.16%), Query Frame = 0

Query: 78   SLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDLVNVRSASASGVVSA--A 137
            +L+    G ++ WG +H  QLG G    +     P       L  +R     G      A
Sbjct: 3945 TLSAGGSGTIYGWGHNHRGQLG-GIEGAKVKVPTPCEA----LATLRPVQLIGGEQTLFA 4004

Query: 138  IGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVSFGWG--HALACSEDG 197
            +  DG L+  G    G+LG+G        P+ +++++   I KV+   G  H LA S +G
Sbjct: 4005 VTADGKLYATGYGAGGRLGIG-GTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEG 4064

Query: 198  KLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERLVLESMKDEKNMPI 257
            +++ WG   DG+LG+  R    SP D                                  
Sbjct: 4065 EVYSWGEAEDGKLGHGNR----SPCD---------------------------------- 4124

Query: 258  IWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGRARQDLGLLP---- 317
               P  +E L G+EVVD+A G  HS  +   G L + G   YG+LG +  +  L P    
Sbjct: 4125 --RPRVIESLRGIEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVE 4184

Query: 318  VDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTSQLGREGPN--SIPLAI 377
                  V  +A G G +  LC       + D  +V SWG     +LGR G +   +P+ I
Sbjct: 4185 ALQGHRVVDIACGSGDAQTLC-------LTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKI 4244

Query: 378  EELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLDSLENCNV 437
            + L G   V V  G   S+ L   G V+ WG G   RLG GS      P  +  L+   V
Sbjct: 4245 DSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKV 4267

Query: 438  VQAVSGFDHNLVLVAD 444
            +   +G  H +    D
Sbjct: 4305 IAIATGSLHCVCCTED 4267

BLAST of MS012739 vs. ExPASy TrEMBL
Match: A0A6J1C6L5 (secretion-regulating guanine nucleotide exchange factor OS=Momordica charantia OX=3673 GN=LOC111008450 PE=4 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.2e-257
Identity = 442/443 (99.77%), Postives = 442/443 (99.77%), Query Frame = 0

Query: 1   MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT 60
           MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT
Sbjct: 1   MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT 60

Query: 61  VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL 120
           VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL
Sbjct: 61  VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL 120

Query: 121 VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS 180
           VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS
Sbjct: 121 VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS 180

Query: 181 FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL 240
           FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL
Sbjct: 181 FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL 240

Query: 241 VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300
           VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR
Sbjct: 241 VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300

Query: 301 ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTSQLGREGP 360
           ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQT QLGREGP
Sbjct: 301 ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTPQLGREGP 360

Query: 361 NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD 420
           NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD
Sbjct: 361 NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD 420

Query: 421 SLENCNVVQAVSGFDHNLVLVAD 444
           SLENCNVVQAVSGFDHNLVLVAD
Sbjct: 421 SLENCNVVQAVSGFDHNLVLVAD 443

BLAST of MS012739 vs. ExPASy TrEMBL
Match: A0A6J1JB03 (ultraviolet-B receptor UVR8 OS=Cucurbita maxima OX=3661 GN=LOC111482841 PE=4 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.2e-229
Identity = 392/443 (88.49%), Postives = 414/443 (93.45%), Query Frame = 0

Query: 1   MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT 60
           MIRGIRILA  Q RA+SI+P+GRRC+ S AVMSFGDGSHGAIGLPTS+TGIGGAAYEP T
Sbjct: 1   MIRGIRILAGEQRRALSIIPFGRRCLSSRAVMSFGDGSHGAIGLPTSLTGIGGAAYEPTT 60

Query: 61  VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL 120
           VPGLPPDVTSVAAGHYHSLA+DSQG LWAWGRD EAQLGRGFASPRDSWNVP RVIGLDL
Sbjct: 61  VPGLPPDVTSVAAGHYHSLAIDSQGCLWAWGRDQEAQLGRGFASPRDSWNVPNRVIGLDL 120

Query: 121 VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS 180
           VNVRSASASGVVSAA+GDDGSLWIWGKSKRGQLGLGKDV EAV PSRVQA+EGE+IVKVS
Sbjct: 121 VNVRSASASGVVSAALGDDGSLWIWGKSKRGQLGLGKDVTEAVLPSRVQAVEGERIVKVS 180

Query: 181 FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL 240
           FGWGHALA SEDGKLFGWGYYADGRLGN+GRTLKMSPLDSSS  STNGQ+   I+LAERL
Sbjct: 181 FGWGHALALSEDGKLFGWGYYADGRLGNIGRTLKMSPLDSSSYRSTNGQEQPNIQLAERL 240

Query: 241 VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300
           VLESM+DEKNMPIIWEPH VEDLTG+EVVDIACGLDHSLVLCRDGTLLSCGSN+YGQLGR
Sbjct: 241 VLESMEDEKNMPIIWEPHLVEDLTGIEVVDIACGLDHSLVLCRDGTLLSCGSNVYGQLGR 300

Query: 301 ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTSQLGREGP 360
            RQDLGLLPVD+SFHVKSVAAGLGHSLAL QDTSN+++ D  SVISWGWNQTSQLGREG 
Sbjct: 301 VRQDLGLLPVDSSFHVKSVAAGLGHSLALYQDTSNKDVSDATSVISWGWNQTSQLGREGQ 360

Query: 361 NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD 420
           NSIPL IE LAGEIP+SVSGGRAHSI L SRGEVWVWGCG+DGRLGLGSSSDESEP+FLD
Sbjct: 361 NSIPLIIEGLAGEIPISVSGGRAHSIALSSRGEVWVWGCGRDGRLGLGSSSDESEPVFLD 420

Query: 421 SLENCNVVQAVSGFDHNLVLVAD 444
           SLENCNVVQAVSGFDHNLVLV D
Sbjct: 421 SLENCNVVQAVSGFDHNLVLVVD 443

BLAST of MS012739 vs. ExPASy TrEMBL
Match: A0A6J1EMP4 (ultraviolet-B receptor UVR8 OS=Cucurbita moschata OX=3662 GN=LOC111435986 PE=4 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 2.0e-229
Identity = 391/443 (88.26%), Postives = 416/443 (93.91%), Query Frame = 0

Query: 1   MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT 60
           MIRGIRILA  Q RA+SI+P+GRRC+ S AVMSFGDGSHGAIGLPTS+TGIGGAAYEP T
Sbjct: 1   MIRGIRILAGEQRRALSIIPFGRRCLSSRAVMSFGDGSHGAIGLPTSLTGIGGAAYEPTT 60

Query: 61  VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL 120
           VP LPPDVTSVAAGHYHSLA+DSQG LWAWGRD EAQLGRGFASPRDSWNVP RVIGLDL
Sbjct: 61  VPVLPPDVTSVAAGHYHSLAIDSQGCLWAWGRDQEAQLGRGFASPRDSWNVPNRVIGLDL 120

Query: 121 VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS 180
           VNVRSASASGVVSAA+GDDGSLWIWG+SKRGQLGLGKDV EAV PSRVQA+EGE+IVKVS
Sbjct: 121 VNVRSASASGVVSAALGDDGSLWIWGRSKRGQLGLGKDVTEAVIPSRVQAVEGERIVKVS 180

Query: 181 FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL 240
           FGWGHALA SEDGKLFGWGYYADGRLGN+G+TLKMSPLDSSS  STNGQ+   I+LAERL
Sbjct: 181 FGWGHALALSEDGKLFGWGYYADGRLGNIGKTLKMSPLDSSSYRSTNGQEQPNIQLAERL 240

Query: 241 VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300
           VLESM+DEKNMPIIWEPH VEDLTG+EVVDIACGLDHSLVLCRDGTLLSCGSN+YGQLGR
Sbjct: 241 VLESMEDEKNMPIIWEPHLVEDLTGIEVVDIACGLDHSLVLCRDGTLLSCGSNVYGQLGR 300

Query: 301 ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTSQLGREGP 360
            RQDLGLLPVD+SFHVKSVAAGLGHSLAL QDTSN+EI DV S+ISWGWNQTSQLGREGP
Sbjct: 301 VRQDLGLLPVDSSFHVKSVAAGLGHSLALYQDTSNKEISDVTSIISWGWNQTSQLGREGP 360

Query: 361 NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD 420
           N+IPLAIE LAGEIP+SVSGGRAHSI L SRGEVWVWGCG+DGRLGLGSSSDESEP+FLD
Sbjct: 361 NNIPLAIEGLAGEIPISVSGGRAHSIALSSRGEVWVWGCGRDGRLGLGSSSDESEPVFLD 420

Query: 421 SLENCNVVQAVSGFDHNLVLVAD 444
           SLENCNV+QAVSGFDHNLVLV D
Sbjct: 421 SLENCNVMQAVSGFDHNLVLVVD 443

BLAST of MS012739 vs. ExPASy TrEMBL
Match: A0A5D3CZE0 (Putative E3 ubiquitin-protein ligase HERC4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004890 PE=4 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.3e-224
Identity = 384/443 (86.68%), Postives = 408/443 (92.10%), Query Frame = 0

Query: 1   MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT 60
           MI G+RILAR Q R +SI+ +G+RCI S AVMSFGDGSHGAIGLPTS+TG+GGAAYEP T
Sbjct: 1   MIPGVRILAREQQRGISIIHFGKRCISSRAVMSFGDGSHGAIGLPTSLTGVGGAAYEPTT 60

Query: 61  VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL 120
           VPGLP  VTSVAAGHYHSLAVDSQG LWAWGRDHEAQLGRGFAS RDSWNVPKRVIGLD 
Sbjct: 61  VPGLPSHVTSVAAGHYHSLAVDSQGCLWAWGRDHEAQLGRGFASSRDSWNVPKRVIGLDQ 120

Query: 121 VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS 180
           V + SA ASGVVSAA+GDDGSLWIWGKSKRGQLGLGKDV EAV PSRVQAL GEKIVKVS
Sbjct: 121 VKISSAFASGVVSAALGDDGSLWIWGKSKRGQLGLGKDVTEAVIPSRVQALGGEKIVKVS 180

Query: 181 FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL 240
           FGWGHALA SEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSS  STNGQ+  KIELAERL
Sbjct: 181 FGWGHALAFSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSYRSTNGQEQPKIELAERL 240

Query: 241 VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300
           VLESM+DEK+MPIIWEPH VED+TGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR
Sbjct: 241 VLESMEDEKHMPIIWEPHLVEDVTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300

Query: 301 ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTSQLGREGP 360
             QDLGLLPVD+SFHVKS+AAGLGHSLA+CQDTSN++ +D  SVISWGWNQTSQLGR GP
Sbjct: 301 VTQDLGLLPVDSSFHVKSIAAGLGHSLAMCQDTSNKDKKDATSVISWGWNQTSQLGRVGP 360

Query: 361 NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD 420
           NSIPLAIE LAGE P+S+SGGRAHSI LGS+GEVWVWGCG+DGRLGLGSSSDESEP+FLD
Sbjct: 361 NSIPLAIEGLAGETPISLSGGRAHSIALGSKGEVWVWGCGRDGRLGLGSSSDESEPVFLD 420

Query: 421 SLENCNVVQAVSGFDHNLVLVAD 444
           SLENCNVVQAVSGFDHNLVLV D
Sbjct: 421 SLENCNVVQAVSGFDHNLVLVVD 443

BLAST of MS012739 vs. ExPASy TrEMBL
Match: A0A1S3BTT0 (probable E3 ubiquitin-protein ligase HERC4 OS=Cucumis melo OX=3656 GN=LOC103493186 PE=4 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.3e-224
Identity = 384/443 (86.68%), Postives = 408/443 (92.10%), Query Frame = 0

Query: 1   MIRGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIGLPTSITGIGGAAYEPIT 60
           MI G+RILAR Q R +SI+ +G+RCI S AVMSFGDGSHGAIGLPTS+TG+GGAAYEP T
Sbjct: 1   MIPGVRILAREQQRGISIIHFGKRCISSRAVMSFGDGSHGAIGLPTSLTGVGGAAYEPTT 60

Query: 61  VPGLPPDVTSVAAGHYHSLAVDSQGHLWAWGRDHEAQLGRGFASPRDSWNVPKRVIGLDL 120
           VPGLP  VTSVAAGHYHSLAVDSQG LWAWGRDHEAQLGRGFAS RDSWNVPKRVIGLD 
Sbjct: 61  VPGLPSHVTSVAAGHYHSLAVDSQGCLWAWGRDHEAQLGRGFASSRDSWNVPKRVIGLDQ 120

Query: 121 VNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEGEKIVKVS 180
           V + SA ASGVVSAA+GDDGSLWIWGKSKRGQLGLGKDV EAV PSRVQAL GEKIVKVS
Sbjct: 121 VKISSAFASGVVSAALGDDGSLWIWGKSKRGQLGLGKDVTEAVIPSRVQALGGEKIVKVS 180

Query: 181 FGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQKIELAERL 240
           FGWGHALA SEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSS  STNGQ+  KIELAERL
Sbjct: 181 FGWGHALAFSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSYRSTNGQEQPKIELAERL 240

Query: 241 VLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300
           VLESM+DEK+MPIIWEPH VED+TGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR
Sbjct: 241 VLESMEDEKHMPIIWEPHLVEDVTGVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGR 300

Query: 301 ARQDLGLLPVDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWGWNQTSQLGREGP 360
             QDLGLLPVD+SFHVKS+AAGLGHSLA+CQDTSN++ +D  SVISWGWNQTSQLGR GP
Sbjct: 301 VTQDLGLLPVDSSFHVKSIAAGLGHSLAMCQDTSNKDKKDATSVISWGWNQTSQLGRVGP 360

Query: 361 NSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLD 420
           NSIPLAIE LAGE P+S+SGGRAHSI LGS+GEVWVWGCG+DGRLGLGSSSDESEP+FLD
Sbjct: 361 NSIPLAIEGLAGETPISLSGGRAHSIALGSKGEVWVWGCGRDGRLGLGSSSDESEPVFLD 420

Query: 421 SLENCNVVQAVSGFDHNLVLVAD 444
           SLENCNVVQAVSGFDHNLVLV D
Sbjct: 421 SLENCNVVQAVSGFDHNLVLVVD 443

BLAST of MS012739 vs. TAIR 10
Match: AT5G48330.2 (Regulator of chromosome condensation (RCC1) family protein )

HSP 1 Score: 493.4 bits (1269), Expect = 1.9e-139
Identity = 250/417 (59.95%), Postives = 309/417 (74.10%), Query Frame = 0

Query: 31  VMSFGDGSHGAIGL-PTSITGIGGAAYEPITVPGLPPDVTSVAAGHYHSLAVDSQGHLWA 90
           VMSFGDG+ GA+GL  +SI G+G  AYEP  V  LP D++S++AGHYHSLAV S G +WA
Sbjct: 46  VMSFGDGNLGALGLSSSSIPGMGMDAYEPTVVSNLPSDISSISAGHYHSLAVTSSGEIWA 105

Query: 91  WGRDHEAQLGRGFASPRDSWNVPKRVIGLDLVNVRSASASGVVSAAIGDDGSLWIWGKSK 150
           WGR+ E QLGR     RDS + PKRV GL+ VNV++A ASGVVSAAIGDDGSLW+WG+SK
Sbjct: 106 WGRNDEGQLGRIVIDSRDSRSEPKRVEGLENVNVQAAFASGVVSAAIGDDGSLWVWGRSK 165

Query: 151 RGQLGLGKDVMEAVRPSRVQALEGEKIVKVSFGWGHALACSEDGKLFGWGYYADGRLGNV 210
           RGQLGLG  ++EA  PSRV+ L  E +VKVS GWGHALA + DGK+FGWGY ADGR+GNV
Sbjct: 166 RGQLGLGNGIIEARVPSRVENLAAEHVVKVSLGWGHALALTVDGKVFGWGYVADGRVGNV 225

Query: 211 GRTLKMSPLDSSSCTSTNGQKLQ---KIELAERLVLESMKDEKNMPIIWEPHFVEDLTGV 270
           G  L+ S LDS +  S  G        +E AE+ V+E+M  E +MPI WEP  VE+    
Sbjct: 226 GLPLEASLLDSITDGSMKGHHAAGDLNLEAAEKKVVEAMSKENDMPIAWEPCLVEETCNE 285

Query: 271 EVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGRARQDLGLLPVDTSFHVKSVAAGLGHS 330
           +V DIACG DHSL+LC DGTLLS GSNIYGQLGR++QDLG+ PVD +    S+AAGLGHS
Sbjct: 286 KVADIACGSDHSLILCHDGTLLSAGSNIYGQLGRSKQDLGMKPVDITESPISIAAGLGHS 345

Query: 331 LALCQDTSNREIRDVVSVISWGWNQTSQLGREGPNSIPLAIEELAGEIPVSVSGGRAHSI 390
           LA+C    NR  R   +++SWGWN++ QLGR  P ++P  +E   GE P SVS GR HS+
Sbjct: 346 LAIC----NRGER---NILSWGWNRSRQLGRGKPENLPREVEGFDGESPASVSAGRVHSL 405

Query: 391 VLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLDSLENCNVVQAVSGFDHNLVLVAD 444
            +  +GE WVWGCGK+GRLGLGSSSDE EP+ L+ +E C V+QAV+GFDH+L+LVA+
Sbjct: 406 CVTEKGEAWVWGCGKNGRLGLGSSSDEPEPMLLEDVEGC-VLQAVAGFDHSLILVAE 454

BLAST of MS012739 vs. TAIR 10
Match: AT5G48330.1 (Regulator of chromosome condensation (RCC1) family protein )

HSP 1 Score: 490.7 bits (1262), Expect = 1.2e-138
Identity = 251/418 (60.05%), Postives = 310/418 (74.16%), Query Frame = 0

Query: 31  VMSFGDGSHGAIGL-PTSITGIGGAAYEPITVPGLPPDVTSVAAGHYHSLAVDSQGHLWA 90
           VMSFGDG+ GA+GL  +SI G+G  AYEP  V  LP D++S++AGHYHSLAV S G +WA
Sbjct: 46  VMSFGDGNLGALGLSSSSIPGMGMDAYEPTVVSNLPSDISSISAGHYHSLAVTSSGEIWA 105

Query: 91  WGRDHEAQLGR-GFASPRDSWNVPKRVIGLDLVNVRSASASGVVSAAIGDDGSLWIWGKS 150
           WGR+ E QLGR    S RDS + PKRV GL+ VNV++A ASGVVSAAIGDDGSLW+WG+S
Sbjct: 106 WGRNDEGQLGRIVIDSSRDSRSEPKRVEGLENVNVQAAFASGVVSAAIGDDGSLWVWGRS 165

Query: 151 KRGQLGLGKDVMEAVRPSRVQALEGEKIVKVSFGWGHALACSEDGKLFGWGYYADGRLGN 210
           KRGQLGLG  ++EA  PSRV+ L  E +VKVS GWGHALA + DGK+FGWGY ADGR+GN
Sbjct: 166 KRGQLGLGNGIIEARVPSRVENLAAEHVVKVSLGWGHALALTVDGKVFGWGYVADGRVGN 225

Query: 211 VGRTLKMSPLDSSSCTSTNGQKLQ---KIELAERLVLESMKDEKNMPIIWEPHFVEDLTG 270
           VG  L+ S LDS +  S  G        +E AE+ V+E+M  E +MPI WEP  VE+   
Sbjct: 226 VGLPLEASLLDSITDGSMKGHHAAGDLNLEAAEKKVVEAMSKENDMPIAWEPCLVEETCN 285

Query: 271 VEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGRARQDLGLLPVDTSFHVKSVAAGLGH 330
            +V DIACG DHSL+LC DGTLLS GSNIYGQLGR++QDLG+ PVD +    S+AAGLGH
Sbjct: 286 EKVADIACGSDHSLILCHDGTLLSAGSNIYGQLGRSKQDLGMKPVDITESPISIAAGLGH 345

Query: 331 SLALCQDTSNREIRDVVSVISWGWNQTSQLGREGPNSIPLAIEELAGEIPVSVSGGRAHS 390
           SLA+C    NR  R   +++SWGWN++ QLGR  P ++P  +E   GE P SVS GR HS
Sbjct: 346 SLAIC----NRGER---NILSWGWNRSRQLGRGKPENLPREVEGFDGESPASVSAGRVHS 405

Query: 391 IVLGSRGEVWVWGCGKDGRLGLGSSSDESEPIFLDSLENCNVVQAVSGFDHNLVLVAD 444
           + +  +GE WVWGCGK+GRLGLGSSSDE EP+ L+ +E C V+QAV+GFDH+L+LVA+
Sbjct: 406 LCVTEKGEAWVWGCGKNGRLGLGSSSDEPEPMLLEDVEGC-VLQAVAGFDHSLILVAE 455

BLAST of MS012739 vs. TAIR 10
Match: AT5G63860.1 (Regulator of chromosome condensation (RCC1) family protein )

HSP 1 Score: 141.0 bits (354), Expect = 2.4e-33
Identity = 113/436 (25.92%), Postives = 189/436 (43.35%), Query Frame = 0

Query: 3   RGIRILARGQSRAVSIVPYGRRCICSGAVMSFGDGSHGAIG--------LPTSITGIGGA 62
           R + I++ G S +V++       +    V S+G G  G +G         PT ++ + G 
Sbjct: 15  RKVLIISAGASHSVAL-------LSGDIVCSWGRGEDGQLGHGDAEDRPSPTQLSALDG- 74

Query: 63  AYEPITVPGLPPDVTSVAAGHYHSLAVDSQG-HLWAWGRDHEAQLGRGFASPRDSWNVPK 122
                        + SV  G  H++A    G  +++WG     +LG G +S       P 
Sbjct: 75  -----------HQIVSVTCGADHTVAYSQSGMEVYSWGWGDFGRLGHGNSS---DLFTPL 134

Query: 123 RVIGLDLVNVRSASASGVVSAAIGDDGSLWIWGKSKRGQLGLGKDVMEAVRPSRVQALEG 182
            +  L  + ++  +       A+  +G +  WG+++ GQLGLG D  +++ P ++QA EG
Sbjct: 135 PIKALHGIRIKQIACGDSHCLAVTMEGEVQSWGRNQNGQLGLG-DTEDSLVPQKIQAFEG 194

Query: 183 EKIVKVSFGWGHALACSEDGKLFGWGYYADGRLGNVGRTLKMSPLDSSSCTSTNGQKLQK 242
            +I  V+ G  H  A +EDG L+GWG+   GR GN+G   +   L     TST G+K+  
Sbjct: 195 IRIKMVAAGAEHTAAVTEDGDLYGWGW---GRYGNLGLGDRTDRLVPERVTSTGGEKMSM 254

Query: 243 IELAERLVLESMKDEKNMPIIWEPHFVEDLTGVEVVDIACGLDHSLVLCRDGTLLSCGSN 302
                                                +ACG  H++ +   G L + G +
Sbjct: 255 -------------------------------------VACGWRHTISVSYSGALYTYGWS 314

Query: 303 IYGQLGRARQDLGLLP-----VDTSFHVKSVAAGLGHSLALCQDTSNREIRDVVSVISWG 362
            YGQLG    +  L+P     +  SF +  ++ G  H++AL  D           +  WG
Sbjct: 315 KYGQLGHGDLEDHLIPHKLEALSNSF-ISQISGGWRHTMALTSDG---------KLYGWG 374

Query: 363 WNQTSQ--LGREGPNSIPLAIEELAGEIPVSVSGGRAHSIVLGSRGEVWVWGCGKDGRLG 422
           WN+  Q  +G       P+ +     +  V VS G  H++ +  R  V+ WG G +G+LG
Sbjct: 375 WNKFGQVGVGNNLDQCSPVQVRFPDDQKVVQVSCGWRHTLAVTERNNVFAWGRGTNGQLG 377

BLAST of MS012739 vs. TAIR 10
Match: AT3G02510.1 (Regulator of chromosome condensation (RCC1) family protein )

HSP 1 Score: 124.0 bits (310), Expect = 3.0e-28
Identity = 106/363 (29.20%), Postives = 159/363 (43.80%), Query Frame = 0

Query: 89  AWGRDHEAQLGRGFASPRDSWNVPKRVIGLDLVNVRSASASGVVSAAIGDDGSLWIWGKS 148
           AWG   + QLG G  +    W     V  L+  +VRS  +    S AI DDG+++ WG +
Sbjct: 9   AWGSGEDGQLGIG-TNEEKEWACV--VEALEPYSVRSVVSGSRNSLAICDDGTMFTWGWN 68

Query: 149 KRGQLGLGKDVMEAVRPSRVQALEGEKIVKVSFGWGHALACSEDGKLFGWGYYADGRLGN 208
           +RG LG   +      PSRV+AL   KI + + G  H LA  + G+ + WG    G+ G 
Sbjct: 69  QRGTLGHQPETKTENIPSRVKALANVKITQAAIGGWHCLAVDDQGRAYAWGGNEYGQCGE 128

Query: 209 VGRTLKMSPLDSSSCTSTNGQKLQKIELAERLVLESMKDEKNMP----IIWEPHFVEDLT 268
                                             E +KDE   P    I+        LT
Sbjct: 129 ----------------------------------EPLKDEMGRPVRRDIVIPKRCAPKLT 188

Query: 269 GVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGRARQDLGLLPVDTSFHVKSVAAGLG 328
              V  +A G  HS+VL R+G + + G       G  +Q    + V    +V+ +A G  
Sbjct: 189 ---VRQVAAGGTHSVVLTREGHVWTWGQP--WPPGDIKQISVPVRVQGLENVRLIAVGAF 248

Query: 329 HSLALCQDTSNREIRDVVSVISWGWNQTSQLGR--EGPNSIPLAIEELAGEIPVSVSGGR 388
           H+LAL +D           +++WG N+  QLG     P S P+ ++ L     V ++ G 
Sbjct: 249 HNLALEEDG---------RLLAWGNNEYGQLGTGDTQPTSHPVPVQGLDDLTLVDIAAGG 308

Query: 389 AHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESE--PIFLDSLENCNVVQAVSGFDHNLVL 444
            HS  L  +GEV+ WG G+ GRLGLG +   S+  P  ++ L   +++Q   G  H++ L
Sbjct: 309 WHSTALTDKGEVYGWGRGEHGRLGLGDNDKSSKMVPQKVNLLAEEDIIQVSCGGTHSVAL 320

BLAST of MS012739 vs. TAIR 10
Match: AT3G02510.2 (Regulator of chromosome condensation (RCC1) family protein )

HSP 1 Score: 117.1 bits (292), Expect = 3.6e-26
Identity = 99/335 (29.55%), Postives = 146/335 (43.58%), Query Frame = 0

Query: 89  AWGRDHEAQLGRGFASPRDSWNVPKRVIGLDLVNVRSASASGVVSAAIGDDGSLWIWGKS 148
           AWG   + QLG G  +    W     V  L+  +VRS  +    S AI DDG+++ WG +
Sbjct: 9   AWGSGEDGQLGIG-TNEEKEWACV--VEALEPYSVRSVVSGSRNSLAICDDGTMFTWGWN 68

Query: 149 KRGQLGLGKDVMEAVRPSRVQALEGEKIVKVSFGWGHALACSEDGKLFGWGYYADGRLGN 208
           +RG LG   +      PSRV+AL   KI + + G  H LA  + G+ + WG    G+ G 
Sbjct: 69  QRGTLGHQPETKTENIPSRVKALANVKITQAAIGGWHCLAVDDQGRAYAWGGNEYGQCGE 128

Query: 209 VGRTLKMSPLDSSSCTSTNGQKLQKIELAERLVLESMKDEKNMP----IIWEPHFVEDLT 268
                                             E +KDE   P    I+        LT
Sbjct: 129 ----------------------------------EPLKDEMGRPVRRDIVIPKRCAPKLT 188

Query: 269 GVEVVDIACGLDHSLVLCRDGTLLSCGSNIYGQLGRARQDLGLLPVDTSFHVKSVAAGLG 328
              V  +A G  HS+VL R+G + + G       G  +Q    + V    +V+ +A G  
Sbjct: 189 ---VRQVAAGGTHSVVLTREGHVWTWGQP--WPPGDIKQISVPVRVQGLENVRLIAVGAF 248

Query: 329 HSLALCQDTSNREIRDVVSVISWGWNQTSQLGR--EGPNSIPLAIEELAGEIPVSVSGGR 388
           H+LAL +D           +++WG N+  QLG     P S P+ ++ L     V ++ G 
Sbjct: 249 HNLALEEDG---------RLLAWGNNEYGQLGTGDTQPTSHPVPVQGLDDLTLVDIAAGG 292

Query: 389 AHSIVLGSRGEVWVWGCGKDGRLGLGSSSDESEPI 418
            HS  L  +GEV+ WG G+ GRLGLG +   S+ +
Sbjct: 309 WHSTALTDKGEVYGWGRGEHGRLGLGDNDKSSKMV 292