MS012602 (gene) Bitter gourd (TR) v1

Overview
NameMS012602
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionENTH domain-containing protein
Locationscaffold63: 1966720 .. 1967808 (+)
RNA-Seq ExpressionMS012602
SyntenyMS012602
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTCGCACAAGAAAGCTCAGCGGCCTTATCGGACTCATCAAAGACAAGGCCTCTCAGAGCAAAGCCGCGCTAATCGCCAAGCCCAACATTCTCTCCTTCCAACTCGCCCTCCTACGCGCCACCACCCACGACCCCTACGCGCCGCCCCACGACAAGCACCTCGCCGCCCTTCTCTCCCTCGGCAAAACCTCACGCGCCACCGCCGCCGCCGCCATCGAAGTCCTCATGGACCGCCTCCAGAGCACCCACAACTCCGCCGTCGCCCTCAAGTGCCTCCTCGCCGTCCACCACATCCTCAAACACGGCGGCTTCATTCTCCAAGACCAGCTCTCTGTTTTCCCCTTCACCGGCGGCAGAAATTATCTCAAACTCTCCGATTTCCGGGACAATTCCAGCCCGATTTCTTGGGAGCTTTCCTCTTGGGTCCGCTGGTACGCCCAATACATCGAAACCGTCTTGTCCACCTCCCGAATTTTGGGCTTTTTCGTTGCCTCTTCCTCTTCAATTGAGGAGAGGGAGAAAAAATCAGAGCAGATTTCCGCCGTTTTCAACTCCGATTTGCTCAGAGACACCGAATCTTTGGTGGGTTTAATCGAAGAAACCACCAAGAAGCCTCATTCTTTGCATCTCAACGCCAACAAATTGGTGGACCGGATTCTCGGATTTGTCGCCGACGATTACTTGTCGGCTATGAAGGAAATTTCCGTCCGAGTTACTGAGTTCCACCAGCGCTTGAGTTGCCTGAGTTTCGGCGAATCGGTCGAGTTGGTTTGCGTCTTGAAACGCCTCGAGGATTGTAAAGAGAAGCAATCGTTGGGTATTTCTGCAAAATATGAGATTTTGATGGATGGGTTCTGGGGCTTGATTTGGGAAACCAAGAACTTGATTGGGGAGACCAAGGAAAATCGAGACGGCACCGGCGCCGGCGGTAAATTGATCATGACCACGGCCAGGATGAGCGACTCGGGCCGGTTTATGGAGCGAGCTAATATTTACCGCGACTCGATCCGGTTCGGTTCGGAGAGGTTCGATTTCAACTGCAAACGGATTCCGGTCGTGGGCATAACGGAACCGTATTTAATTAAA

mRNA sequence

ATGGTTCGCACAAGAAAGCTCAGCGGCCTTATCGGACTCATCAAAGACAAGGCCTCTCAGAGCAAAGCCGCGCTAATCGCCAAGCCCAACATTCTCTCCTTCCAACTCGCCCTCCTACGCGCCACCACCCACGACCCCTACGCGCCGCCCCACGACAAGCACCTCGCCGCCCTTCTCTCCCTCGGCAAAACCTCACGCGCCACCGCCGCCGCCGCCATCGAAGTCCTCATGGACCGCCTCCAGAGCACCCACAACTCCGCCGTCGCCCTCAAGTGCCTCCTCGCCGTCCACCACATCCTCAAACACGGCGGCTTCATTCTCCAAGACCAGCTCTCTGTTTTCCCCTTCACCGGCGGCAGAAATTATCTCAAACTCTCCGATTTCCGGGACAATTCCAGCCCGATTTCTTGGGAGCTTTCCTCTTGGGTCCGCTGGTACGCCCAATACATCGAAACCGTCTTGTCCACCTCCCGAATTTTGGGCTTTTTCGTTGCCTCTTCCTCTTCAATTGAGGAGAGGGAGAAAAAATCAGAGCAGATTTCCGCCGTTTTCAACTCCGATTTGCTCAGAGACACCGAATCTTTGGTGGGTTTAATCGAAGAAACCACCAAGAAGCCTCATTCTTTGCATCTCAACGCCAACAAATTGGTGGACCGGATTCTCGGATTTGTCGCCGACGATTACTTGTCGGCTATGAAGGAAATTTCCGTCCGAGTTACTGAGTTCCACCAGCGCTTGAGTTGCCTGAGTTTCGGCGAATCGGTCGAGTTGGTTTGCGTCTTGAAACGCCTCGAGGATTGTAAAGAGAAGCAATCGTTGGGTATTTCTGCAAAATATGAGATTTTGATGGATGGGTTCTGGGGCTTGATTTGGGAAACCAAGAACTTGATTGGGGAGACCAAGGAAAATCGAGACGGCACCGGCGCCGGCGGTAAATTGATCATGACCACGGCCAGGATGAGCGACTCGGGCCGGTTTATGGAGCGAGCTAATATTTACCGCGACTCGATCCGGTTCGGTTCGGAGAGGTTCGATTTCAACTGCAAACGGATTCCGGTCGTGGGCATAACGGAACCGTATTTAATTAAA

Coding sequence (CDS)

ATGGTTCGCACAAGAAAGCTCAGCGGCCTTATCGGACTCATCAAAGACAAGGCCTCTCAGAGCAAAGCCGCGCTAATCGCCAAGCCCAACATTCTCTCCTTCCAACTCGCCCTCCTACGCGCCACCACCCACGACCCCTACGCGCCGCCCCACGACAAGCACCTCGCCGCCCTTCTCTCCCTCGGCAAAACCTCACGCGCCACCGCCGCCGCCGCCATCGAAGTCCTCATGGACCGCCTCCAGAGCACCCACAACTCCGCCGTCGCCCTCAAGTGCCTCCTCGCCGTCCACCACATCCTCAAACACGGCGGCTTCATTCTCCAAGACCAGCTCTCTGTTTTCCCCTTCACCGGCGGCAGAAATTATCTCAAACTCTCCGATTTCCGGGACAATTCCAGCCCGATTTCTTGGGAGCTTTCCTCTTGGGTCCGCTGGTACGCCCAATACATCGAAACCGTCTTGTCCACCTCCCGAATTTTGGGCTTTTTCGTTGCCTCTTCCTCTTCAATTGAGGAGAGGGAGAAAAAATCAGAGCAGATTTCCGCCGTTTTCAACTCCGATTTGCTCAGAGACACCGAATCTTTGGTGGGTTTAATCGAAGAAACCACCAAGAAGCCTCATTCTTTGCATCTCAACGCCAACAAATTGGTGGACCGGATTCTCGGATTTGTCGCCGACGATTACTTGTCGGCTATGAAGGAAATTTCCGTCCGAGTTACTGAGTTCCACCAGCGCTTGAGTTGCCTGAGTTTCGGCGAATCGGTCGAGTTGGTTTGCGTCTTGAAACGCCTCGAGGATTGTAAAGAGAAGCAATCGTTGGGTATTTCTGCAAAATATGAGATTTTGATGGATGGGTTCTGGGGCTTGATTTGGGAAACCAAGAACTTGATTGGGGAGACCAAGGAAAATCGAGACGGCACCGGCGCCGGCGGTAAATTGATCATGACCACGGCCAGGATGAGCGACTCGGGCCGGTTTATGGAGCGAGCTAATATTTACCGCGACTCGATCCGGTTCGGTTCGGAGAGGTTCGATTTCAACTGCAAACGGATTCCGGTCGTGGGCATAACGGAACCGTATTTAATTAAA

Protein sequence

MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLSLGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGRNYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKSEQISAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRVTEFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGETKENRDGTGAGGKLIMTTARMSDSGRFMERANIYRDSIRFGSERFDFNCKRIPVVGITEPYLIK
Homology
BLAST of MS012602 vs. NCBI nr
Match: XP_022136401.1 (putative clathrin assembly protein At4g40080 [Momordica charantia])

HSP 1 Score: 676.8 bits (1745), Expect = 1.0e-190
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS 60
           MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS
Sbjct: 1   MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS 60

Query: 61  LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR 120
           LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR
Sbjct: 61  LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR 120

Query: 121 NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKSEQI 180
           NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKSEQI
Sbjct: 121 NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKSEQI 180

Query: 181 SAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRVT 240
           SAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRVT
Sbjct: 181 SAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRVT 240

Query: 241 EFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGET 300
           EFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGET
Sbjct: 241 EFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGET 300

Query: 301 KENRDGTGAGGKLIMTTARMSDSGRFMERANIYRDSIRFGSERFDF 347
           KENRDGTGAGGKLIMTTARMSDSGRFMERANIYRDSIRFGSERFDF
Sbjct: 301 KENRDGTGAGGKLIMTTARMSDSGRFMERANIYRDSIRFGSERFDF 346

BLAST of MS012602 vs. NCBI nr
Match: XP_038903242.1 (putative clathrin assembly protein At4g40080 [Benincasa hispida])

HSP 1 Score: 573.5 bits (1477), Expect = 1.2e-159
Identity = 291/364 (79.95%), Postives = 320/364 (87.91%), Query Frame = 0

Query: 1   MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS 60
           MV T+ LS LIGLIKDKASQSKAAL+AKPNILSFQLALLRATTHDP+APP +KHL  LLS
Sbjct: 1   MVSTKNLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDPHAPPREKHLCVLLS 60

Query: 61  LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR 120
           LGKTSRATAAAA+EVLMDRLQ+T NSAVALKCL+AVHHI+K+GGFILQDQLSVFPFTGGR
Sbjct: 61  LGKTSRATAAAAVEVLMDRLQTTQNSAVALKCLIAVHHIVKNGGFILQDQLSVFPFTGGR 120

Query: 121 NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKSEQI 180
           NYLKLSDFRD+S+PISWELSSWVRWYAQYIETVLS SRILGFFV SS+S EE+EKK+EQI
Sbjct: 121 NYLKLSDFRDSSNPISWELSSWVRWYAQYIETVLSISRILGFFVGSSTSNEEKEKKTEQI 180

Query: 181 SAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRVT 240
           S + NSDLL++TESLVGLIEET+K PH LHLN N+L D+I  FV DDYLSAMKEIS+RVT
Sbjct: 181 SGILNSDLLKETESLVGLIEETSKMPHCLHLNGNRLADKIYAFVGDDYLSAMKEISIRVT 240

Query: 241 EFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGET 300
           EFHQRLSCLSFGESVELVC LKRLEDCKEKQS GIS+KYE+LMD FWG I ETKNLIGE+
Sbjct: 241 EFHQRLSCLSFGESVELVCALKRLEDCKEKQSKGISSKYEVLMDEFWGSIRETKNLIGES 300

Query: 301 KENRDGTGAGGKLIMTTARMSDSGRFMER--ANIYRDSIRFGSERFDFNCKRIPVVGITE 360
           KEN++    GGKL  T +RMSDSGRFMER  A  YRDS+RFGSERFD  CK  PV G  E
Sbjct: 301 KENQE----GGKLARTKSRMSDSGRFMERAIAGSYRDSLRFGSERFDLTCKGFPVPGTRE 360

Query: 361 PYLI 363
            Y +
Sbjct: 361 SYFL 360

BLAST of MS012602 vs. NCBI nr
Match: KAG7015410.1 (putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 544.7 bits (1402), Expect = 6.2e-151
Identity = 283/365 (77.53%), Postives = 314/365 (86.03%), Query Frame = 0

Query: 1   MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS 60
           MVRT+KLS LIGLIKDKASQSKAAL+AKPNILSFQLALLRATTHDP+APP  K L+ LLS
Sbjct: 1   MVRTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDPHAPPTHKDLSVLLS 60

Query: 61  LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR 120
           LGKTSRATAAAA+EVLMDRLQST NSAVALKCL+A+HHI+K+G FILQDQLSVFPFTGGR
Sbjct: 61  LGKTSRATAAAALEVLMDRLQSTQNSAVALKCLIAIHHIIKNGDFILQDQLSVFPFTGGR 120

Query: 121 NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILG-FFVASSSSIEEREKKSEQ 180
           NYLKLSDFRD+S+PISWELSSWVRWYAQYIETVL  SRILG FFV SSSS  EREKK+EQ
Sbjct: 121 NYLKLSDFRDSSNPISWELSSWVRWYAQYIETVLCISRILGFFFVGSSSSNAEREKKTEQ 180

Query: 181 ISAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRV 240
           IS  FNSDLL++TESL+GLIEE +K PH LHLN N LVD+I  FV +DYLSA KEIS RV
Sbjct: 181 ISGFFNSDLLKETESLMGLIEEVSKMPHCLHLNGNGLVDKIYAFVGEDYLSATKEISTRV 240

Query: 241 TEFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGE 300
           TEF QRL CLSFGESVELVC LKRLEDCKEKQS GIS  +EIL+ GFWG I E +NLIGE
Sbjct: 241 TEFRQRLGCLSFGESVELVCALKRLEDCKEKQSRGISGNHEILLKGFWGSIREIRNLIGE 300

Query: 301 TKENRDGTGAGGKLIMTTARMSDSGRFMER--ANIYRDSIRFGSERFDFNCKRIPVVGIT 360
           +K+ R+     GKL  T +RMSDSGRFM++  A +YR S+RFGSERFDF CK IPV+GIT
Sbjct: 301 SKDPRE----TGKLGRTKSRMSDSGRFMDQDNAKLYRHSVRFGSERFDFTCKGIPVLGIT 360

Query: 361 EPYLI 363
           E YL+
Sbjct: 361 ESYLL 361

BLAST of MS012602 vs. NCBI nr
Match: XP_023552000.1 (putative clathrin assembly protein At4g40080 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 544.3 bits (1401), Expect = 8.1e-151
Identity = 282/365 (77.26%), Postives = 315/365 (86.30%), Query Frame = 0

Query: 1   MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS 60
           MVRT+KLS LIGLIKDKASQSKAAL+AKPNI+SFQLALLRATTHDP+APP  K L+ LLS
Sbjct: 32  MVRTKKLSSLIGLIKDKASQSKAALLAKPNIVSFQLALLRATTHDPHAPPTHKDLSVLLS 91

Query: 61  LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR 120
           LGKTSRATAAAA+EVLMDRLQST NSAVALKCL+A+HHI+K+G FILQDQLSVFPFTGGR
Sbjct: 92  LGKTSRATAAAALEVLMDRLQSTQNSAVALKCLIAIHHIIKNGDFILQDQLSVFPFTGGR 151

Query: 121 NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILG-FFVASSSSIEEREKKSEQ 180
           NYLKLSDFRD+S+PISWELSSWVRWYAQYIETVL  SRILG FFV SSSS  EREKK+EQ
Sbjct: 152 NYLKLSDFRDSSNPISWELSSWVRWYAQYIETVLCISRILGFFFVGSSSSNAEREKKTEQ 211

Query: 181 ISAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRV 240
           IS  FNSDLL++TESL+GLIEE +K PH LHLN N LVD+I  FV +DYLSA KEIS RV
Sbjct: 212 ISGFFNSDLLKETESLMGLIEEVSKIPHCLHLNGNGLVDKIYAFVGEDYLSATKEISTRV 271

Query: 241 TEFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGE 300
           TEF QRL CLSFGESVELVC LKRLEDCKEKQS GIS  +EIL+ GFWG I E +NLIGE
Sbjct: 272 TEFRQRLGCLSFGESVELVCALKRLEDCKEKQSRGISGNHEILLKGFWGSIREIRNLIGE 331

Query: 301 TKENRDGTGAGGKLIMTTARMSDSGRFMER--ANIYRDSIRFGSERFDFNCKRIPVVGIT 360
           +K++R+     GKL  T +RMSDSGRFM++  A +YR S+RFGSERFDF CK IPV+GIT
Sbjct: 332 SKDHRE----TGKLDRTKSRMSDSGRFMDQDNAKLYRHSVRFGSERFDFTCKGIPVLGIT 391

Query: 361 EPYLI 363
           E YL+
Sbjct: 392 ESYLL 392

BLAST of MS012602 vs. NCBI nr
Match: XP_022929539.1 (putative clathrin assembly protein At4g40080 [Cucurbita moschata])

HSP 1 Score: 543.5 bits (1399), Expect = 1.4e-150
Identity = 283/365 (77.53%), Postives = 314/365 (86.03%), Query Frame = 0

Query: 1   MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS 60
           MVRT+KLS LIGLIKDKASQSKAAL+AKPNILSFQLALLRATTHDP+APP  K L+ LLS
Sbjct: 32  MVRTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDPHAPPTHKDLSVLLS 91

Query: 61  LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR 120
           LGKTSRATAAAA+EVLMDRLQST NSAVALKCL+A+HHI+K+G FILQDQLSVFPFTGGR
Sbjct: 92  LGKTSRATAAAALEVLMDRLQSTQNSAVALKCLIAIHHIIKNGDFILQDQLSVFPFTGGR 151

Query: 121 NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILG-FFVASSSSIEEREKKSEQ 180
           NYLKLSDFRD+S+PISWELSSWVRWYAQYIETVL  SRILG FFV SSSS  EREKK+EQ
Sbjct: 152 NYLKLSDFRDSSNPISWELSSWVRWYAQYIETVLCISRILGFFFVGSSSSNAEREKKTEQ 211

Query: 181 ISAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRV 240
           IS  FNSDLL++TESL+GLIEE +K PH LHLN N LVD+I  FV +DYLSA KEIS RV
Sbjct: 212 ISGFFNSDLLKETESLMGLIEEVSKMPHCLHLNGNGLVDKIYAFVGEDYLSATKEISNRV 271

Query: 241 TEFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGE 300
           TEF QRL CLSFGESVELVC LKRLEDCKEKQS GIS  +EIL+ GFWG I E +NLIGE
Sbjct: 272 TEFRQRLGCLSFGESVELVCALKRLEDCKEKQSRGISGNHEILLKGFWGSIREIRNLIGE 331

Query: 301 TKENRDGTGAGGKLIMTTARMSDSGRFMER--ANIYRDSIRFGSERFDFNCKRIPVVGIT 360
           +K+ R+     GKL  T +RMSDSGRFM++  A +YR S+RFGSERFDF CK IPV+GIT
Sbjct: 332 SKDPRE----TGKLGRTKSRMSDSGRFMDQDNAKLYRHSVRFGSERFDFTCKGIPVLGIT 391

Query: 361 EPYLI 363
           E YL+
Sbjct: 392 ESYLL 392

BLAST of MS012602 vs. ExPASy Swiss-Prot
Match: Q8L936 (Putative clathrin assembly protein At4g40080 OS=Arabidopsis thaliana OX=3702 GN=At4g40080 PE=2 SV=2)

HSP 1 Score: 333.6 bits (854), Expect = 2.9e-90
Identity = 181/352 (51.42%), Postives = 242/352 (68.75%), Query Frame = 0

Query: 1   MVRTRKLSGLIGLIKDKASQSKAALIA---KPNILSFQLALLRATTHDPYAPPHDKHLAA 60
           M R    + LIG IKDKASQSKAAL++   K   LSF L++LRATTHDP  PP ++HLA 
Sbjct: 1   MGRITSFADLIGRIKDKASQSKAALVSSNTKSKTLSFHLSVLRATTHDPSTPPGNRHLAV 60

Query: 61  LLSLGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFT 120
           +LS G  SRATA++A+E +M+RL +T ++ VALK L+ +HHI+KHG FILQDQLSVFP +
Sbjct: 61  ILSAGTGSRATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHGRFILQDQLSVFPAS 120

Query: 121 GGRNYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKS 180
           GGRNYLKLS FRD  SP+ WELSSWVRWYA Y+E +LSTSRI+GFF++S+SS   +E+  
Sbjct: 121 GGRNYLKLSAFRDEKSPLMWELSSWVRWYALYLEHLLSTSRIMGFFISSTSSTIHKEEYE 180

Query: 181 EQISAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISV 240
           E +S++ NSDLLR+ ++LVGL+EE  K P         L D+I   V +DY+S++ E+  
Sbjct: 181 EMVSSLTNSDLLREIDALVGLLEEACKIPDLPFSGGKSLADKITQLVGEDYVSSINELYT 240

Query: 241 RVTEFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEI-LMDGFWGLIWETKNL 300
           R  EF +R + LSFG+++ELVC LKRLE CKE+ S      ++   +DGFWGL+ E K +
Sbjct: 241 RFNEFKERSNTLSFGDTIELVCALKRLESCKERLSEICHGNWKRGWIDGFWGLVLEVKGI 300

Query: 301 IGETKENRDGTGAGGKLIMTTARMS---DSGRFMERANI-YRDSIRFGSERF 345
           IG  ++N    G   K I+   +     +S RF +R  I Y + +RF S RF
Sbjct: 301 IGNLEDN---YGQIEKSIVGFGKRDKGYESARFTDRLIIGYSNPVRFSSGRF 349

BLAST of MS012602 vs. ExPASy Swiss-Prot
Match: Q9FKQ2 (Putative clathrin assembly protein At5g65370 OS=Arabidopsis thaliana OX=3702 GN=At5g65370 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.3e-31
Identity = 95/301 (31.56%), Postives = 162/301 (53.82%), Query Frame = 0

Query: 6   KLSGLIGLIKDKASQSK---AALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLSLG 65
           KL+ L G++KD+ASQ K     L +  N  +  LALL+AT+H    PP DK++  L S  
Sbjct: 3   KLATLNGILKDEASQMKLNVVHLCSSVNAKTIDLALLKATSHTSNNPPSDKYVTFLQSTI 62

Query: 66  KTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILK-HGGFILQDQL------SVFP 125
            T        ++ ++ RL+ T +  VA KCL+ +H ++K   G+  +D L          
Sbjct: 63  DT--CYGPDTVDAILHRLRVTTDVCVAAKCLILLHKMVKSESGYNGEDSLRNNINHRTLI 122

Query: 126 FTGGRNYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREK 185
           +T G + LKL+D   NSS  + EL+ WV+WY QY++  LS + +LG         E++  
Sbjct: 123 YTQGGSNLKLNDLNVNSSRFTRELTPWVQWYKQYLDCYLSIAEVLGITPNIKEKNEDKRL 182

Query: 186 KSEQISAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEI 245
           +++++S+     +L+  + LV L E  + +P +     NK+V  +   +  DY SA++ +
Sbjct: 183 ETQRVSSYPMDCILKQIDFLVELFEHISDRPKAPQSKLNKIVIEMTELMVQDYFSAIRLM 242

Query: 246 SVRVTEFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKN 297
            +R  E + R++     +  ELV VL++LE+CKE  S   S + + L+  FW L+ + K+
Sbjct: 243 RIRFEELNVRVA-----KPNELVPVLEKLENCKEGLS-EFSWRSKYLIADFWYLVSKLKD 295

BLAST of MS012602 vs. ExPASy Swiss-Prot
Match: Q8H0W9 (Putative clathrin assembly protein At5g10410 OS=Arabidopsis thaliana OX=3702 GN=At5g10410 PE=2 SV=2)

HSP 1 Score: 137.9 bits (346), Expect = 2.3e-31
Identity = 99/318 (31.13%), Postives = 163/318 (51.26%), Query Frame = 0

Query: 10  LIGLIKDKASQSKAALI---AKPNILSFQLALLRATTHDPYAPPHDKHLAALLSLGKTSR 69
           +IG  KDKAS  KA L+       +    LALL++TT  P  PP+  +++A++S   ++ 
Sbjct: 8   IIGKFKDKASIGKARLVHSFGSTAVKYIHLALLKSTTRTPNKPPNSDYVSAVISY--SNS 67

Query: 70  ATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGRNYLKLS 129
             A AA    + RL+ T N+ VA K L+ +H ++K      +D+        GRN LKL+
Sbjct: 68  RYAPAAFSAALWRLRVTKNAIVATKSLIVIHKLIKSS----RDKFE--GLGHGRNNLKLN 127

Query: 130 DFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKSEQISAVFNS 189
           +F D SS ++ ELS W+RWY QY++ +    ++LG F     + +++ ++ +++S+    
Sbjct: 128 EFSDKSSNLTLELSQWIRWYGQYLDRLSWVPKVLGSFPNLLVNPKDKVEEKDRVSSYQTG 187

Query: 190 DLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRVTEFHQRL 249
            ++R T+SLV   E    +P    +  NK+VD I   V +DY   ++ + VR+    +RL
Sbjct: 188 YIIRQTDSLVSFFEHICTRPEIPPMFQNKIVDEIRELVIEDYFKIVRLVMVRLQVLFERL 247

Query: 250 ---SCLSFGE--SVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGETK 309
                   G+    +   +L RL +CKE  S G+  +   L D FW L+ E      E K
Sbjct: 248 IKPGVKPIGDLGLNDFSLLLVRLVECKESLS-GLFWRCRRLADDFWCLV-EMLKAETEKK 307

Query: 310 ENRDGTGAGGKLIMTTAR 320
            N+      G L+ TT +
Sbjct: 308 NNKQMIELAG-LVQTTVK 314

BLAST of MS012602 vs. ExPASy Swiss-Prot
Match: Q8LBH2 (Putative clathrin assembly protein At2g01600 OS=Arabidopsis thaliana OX=3702 GN=At2g01600 PE=2 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 6.1e-16
Identity = 55/154 (35.71%), Postives = 84/154 (54.55%), Query Frame = 0

Query: 12  GLIKDKASQSKAALI-AKPNILSFQLALLRATTHDPYAPPHDKHLAALLSLGKTSRATA- 71
           G +KD    +K  L+          +A+++AT H    PP D+HL  + +    +RA A 
Sbjct: 12  GALKD---STKVGLVRVNSEYADLDVAIVKATNH-VECPPKDRHLRKIFAATSVTRARAD 71

Query: 72  -AAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGRNYLKLSDF 131
            A  I  L  RL  T N  VALK L+ +H +L+ G    +++L  F   G    L+LS+F
Sbjct: 72  VAYCIHALSRRLHKTRNWTVALKTLIVIHRLLREGDPTFREELLNFSQRG--RILQLSNF 131

Query: 132 RDNSSPISWELSSWVRWYAQYIETVLSTSRILGF 163
           +D+SSPI+W+ S+WVR YA ++E  L   R+L +
Sbjct: 132 KDDSSPIAWDCSAWVRTYALFLEERLECFRVLKY 159

BLAST of MS012602 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 8.8e-15
Identity = 52/147 (35.37%), Postives = 81/147 (55.10%), Query Frame = 0

Query: 6   KLSGLIGLIKDKASQSKAALIAKPNILS-FQLALLRATTHDPYAPPHDKHLAALLSLGKT 65
           KL   IG +KD+ S   A +  + + L+  ++A+++AT HD Y P  DK++  +L L   
Sbjct: 5   KLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDY-PAEDKYIREILCLTSY 64

Query: 66  SRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGRNYLK 125
           SR   +A +  L  RL  T N +VALK L+ +  +L  G    + ++  F    G   L 
Sbjct: 65  SRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEI-FFATRRGTRLLN 124

Query: 126 LSDFRDNSSPISWELSSWVRWYAQYIE 152
           +SDFRD S   SW+ S++VR YA Y++
Sbjct: 125 MSDFRDASQSDSWDYSAFVRTYALYLD 149

BLAST of MS012602 vs. ExPASy TrEMBL
Match: A0A6J1C3E8 (putative clathrin assembly protein At4g40080 OS=Momordica charantia OX=3673 GN=LOC111008121 PE=4 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 5.1e-191
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS 60
           MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS
Sbjct: 1   MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS 60

Query: 61  LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR 120
           LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR
Sbjct: 61  LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR 120

Query: 121 NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKSEQI 180
           NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKSEQI
Sbjct: 121 NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKSEQI 180

Query: 181 SAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRVT 240
           SAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRVT
Sbjct: 181 SAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRVT 240

Query: 241 EFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGET 300
           EFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGET
Sbjct: 241 EFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGET 300

Query: 301 KENRDGTGAGGKLIMTTARMSDSGRFMERANIYRDSIRFGSERFDF 347
           KENRDGTGAGGKLIMTTARMSDSGRFMERANIYRDSIRFGSERFDF
Sbjct: 301 KENRDGTGAGGKLIMTTARMSDSGRFMERANIYRDSIRFGSERFDF 346

BLAST of MS012602 vs. ExPASy TrEMBL
Match: A0A6J1EP16 (putative clathrin assembly protein At4g40080 OS=Cucurbita moschata OX=3662 GN=LOC111436077 PE=4 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 6.7e-151
Identity = 283/365 (77.53%), Postives = 314/365 (86.03%), Query Frame = 0

Query: 1   MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS 60
           MVRT+KLS LIGLIKDKASQSKAAL+AKPNILSFQLALLRATTHDP+APP  K L+ LLS
Sbjct: 32  MVRTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDPHAPPTHKDLSVLLS 91

Query: 61  LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR 120
           LGKTSRATAAAA+EVLMDRLQST NSAVALKCL+A+HHI+K+G FILQDQLSVFPFTGGR
Sbjct: 92  LGKTSRATAAAALEVLMDRLQSTQNSAVALKCLIAIHHIIKNGDFILQDQLSVFPFTGGR 151

Query: 121 NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILG-FFVASSSSIEEREKKSEQ 180
           NYLKLSDFRD+S+PISWELSSWVRWYAQYIETVL  SRILG FFV SSSS  EREKK+EQ
Sbjct: 152 NYLKLSDFRDSSNPISWELSSWVRWYAQYIETVLCISRILGFFFVGSSSSNAEREKKTEQ 211

Query: 181 ISAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRV 240
           IS  FNSDLL++TESL+GLIEE +K PH LHLN N LVD+I  FV +DYLSA KEIS RV
Sbjct: 212 ISGFFNSDLLKETESLMGLIEEVSKMPHCLHLNGNGLVDKIYAFVGEDYLSATKEISNRV 271

Query: 241 TEFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGE 300
           TEF QRL CLSFGESVELVC LKRLEDCKEKQS GIS  +EIL+ GFWG I E +NLIGE
Sbjct: 272 TEFRQRLGCLSFGESVELVCALKRLEDCKEKQSRGISGNHEILLKGFWGSIREIRNLIGE 331

Query: 301 TKENRDGTGAGGKLIMTTARMSDSGRFMER--ANIYRDSIRFGSERFDFNCKRIPVVGIT 360
           +K+ R+     GKL  T +RMSDSGRFM++  A +YR S+RFGSERFDF CK IPV+GIT
Sbjct: 332 SKDPRE----TGKLGRTKSRMSDSGRFMDQDNAKLYRHSVRFGSERFDFTCKGIPVLGIT 391

Query: 361 EPYLI 363
           E YL+
Sbjct: 392 ESYLL 392

BLAST of MS012602 vs. ExPASy TrEMBL
Match: A0A6J1JCT4 (putative clathrin assembly protein At4g40080 OS=Cucurbita maxima OX=3661 GN=LOC111483252 PE=4 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 5.3e-148
Identity = 279/365 (76.44%), Postives = 310/365 (84.93%), Query Frame = 0

Query: 1   MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS 60
           MV T+KLS LIGLIKDKASQSKAAL+AKPNILSFQLALLRATTHDP+APP  K L+ LLS
Sbjct: 37  MVSTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDPHAPPIHKDLSVLLS 96

Query: 61  LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR 120
            GKTSRATAAAA+EVLMDRLQST NSAVALKCL+A+HHI+K+G FILQDQLSVFPFTGGR
Sbjct: 97  FGKTSRATAAAALEVLMDRLQSTQNSAVALKCLIAIHHIVKNGDFILQDQLSVFPFTGGR 156

Query: 121 NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILG-FFVASSSSIEEREKKSEQ 180
           NYLKLSDFRD+S+PISWELSSWVRWYAQYIETVL  SRILG FFV SSSS  EREKK+EQ
Sbjct: 157 NYLKLSDFRDSSNPISWELSSWVRWYAQYIETVLCISRILGFFFVGSSSSNAEREKKTEQ 216

Query: 181 ISAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRV 240
           IS  FNSDLL++TESL+GLIEE +K PH LHLN N LVD+I  FV +DYLSA KEIS RV
Sbjct: 217 ISGFFNSDLLKETESLMGLIEEVSKMPHCLHLNGNGLVDKIYAFVGEDYLSATKEISTRV 276

Query: 241 TEFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGE 300
           TEF  RL CLSFGESVELVC LKRLEDCKEKQS GIS  +EIL+ GFWG I E +NLIGE
Sbjct: 277 TEFRHRLGCLSFGESVELVCALKRLEDCKEKQSRGISGNHEILLKGFWGSIREIRNLIGE 336

Query: 301 TKENRDGTGAGGKLIMTTARMSDSGRFMER--ANIYRDSIRFGSERFDFNCKRIPVVGIT 360
           +K+ R+     GKL  T +RMSDSGRFM++  A + R S+RFGSERFDF CK IPV+GIT
Sbjct: 337 SKDLRE----TGKLGRTKSRMSDSGRFMDQDNAKLDRHSVRFGSERFDFTCKGIPVLGIT 396

Query: 361 EPYLI 363
           E YL+
Sbjct: 397 ESYLL 397

BLAST of MS012602 vs. ExPASy TrEMBL
Match: A0A1S3C1C0 (putative clathrin assembly protein At4g40080 OS=Cucumis melo OX=3656 GN=LOC103495758 PE=4 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 1.0e-146
Identity = 273/351 (77.78%), Postives = 304/351 (86.61%), Query Frame = 0

Query: 1   MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS 60
           M+ T++LS LIGLIKDKASQSKAAL+AKPNILSFQLALLRATTHDP+APP DKHL+ALLS
Sbjct: 1   MMNTKRLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDPHAPPSDKHLSALLS 60

Query: 61  LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR 120
           LGKTSRATAAAA+EVLMDRLQ+THNSAVALKCL+AVHHI K+GGFILQDQLSVFPFTGGR
Sbjct: 61  LGKTSRATAAAAVEVLMDRLQTTHNSAVALKCLIAVHHIFKNGGFILQDQLSVFPFTGGR 120

Query: 121 NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKSEQI 180
           NYLKLSDFRD+S+PISWELSSWVRWYAQYIETVLS SRILGF V SSSS EE E+K+EQI
Sbjct: 121 NYLKLSDFRDSSNPISWELSSWVRWYAQYIETVLSISRILGFIVGSSSSNEEMERKTEQI 180

Query: 181 SAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRVT 240
           S ++NS+LL+DTESLVGLIEE +K P  LHLN N+LVD+I GFV DDYL+AMKEIS+RVT
Sbjct: 181 SGIWNSELLKDTESLVGLIEEISKMPPCLHLNRNRLVDKIYGFVGDDYLAAMKEISIRVT 240

Query: 241 EFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGET 300
           EFH RL CLSFGESVELVC LKRL+D KEKQSLGI A+YE+LMDGFW  I ETKNLIG +
Sbjct: 241 EFHHRLGCLSFGESVELVCALKRLDDFKEKQSLGIFARYEVLMDGFWSSIRETKNLIGAS 300

Query: 301 KENRDGTGAGGKLIMTTARMSDSGRFMERANI--YRDSIRFGSERFDFNCK 350
           KENRDG     KL     R+SDSGRF+ER+N   Y D + F SERF    K
Sbjct: 301 KENRDGC----KLSQMERRISDSGRFIERSNASSYCDVLPFRSERFGLTYK 347

BLAST of MS012602 vs. ExPASy TrEMBL
Match: A0A5A7TT50 (Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold316G00710 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.3e-146
Identity = 271/351 (77.21%), Postives = 304/351 (86.61%), Query Frame = 0

Query: 1   MVRTRKLSGLIGLIKDKASQSKAALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLS 60
           M+ T++LS LIGLIKDKASQSKAAL+AKPNILSFQLALLRATTHDP+APP DKHL+ALLS
Sbjct: 1   MMNTKRLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDPHAPPSDKHLSALLS 60

Query: 61  LGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGR 120
           LGKTSRATAAAA+EVLMDRLQ+THNSAVALKCL+AVHHI K+GGFILQDQLSVFPFTGGR
Sbjct: 61  LGKTSRATAAAAVEVLMDRLQTTHNSAVALKCLIAVHHIFKNGGFILQDQLSVFPFTGGR 120

Query: 121 NYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKSEQI 180
           NYLKLSDFRD+S+PISWELSSWVRWYAQYIETVLS SR LGF V SSSS EE E+K+EQI
Sbjct: 121 NYLKLSDFRDSSNPISWELSSWVRWYAQYIETVLSISRNLGFIVGSSSSNEEMERKTEQI 180

Query: 181 SAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRVT 240
           S ++NS+LL+DTESLVGLIEE +K P  LHLN N+LVD+I GFV DDYL+AMK+IS+RVT
Sbjct: 181 SGIWNSELLKDTESLVGLIEEISKMPPCLHLNRNRLVDKIYGFVGDDYLAAMKDISIRVT 240

Query: 241 EFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGET 300
           EFH RL CLSFGESVELVC LKRL+DCKEKQS+GI A+YE+LMDGFW  I ETKNLIG +
Sbjct: 241 EFHHRLGCLSFGESVELVCALKRLDDCKEKQSMGIFARYEVLMDGFWSSIRETKNLIGAS 300

Query: 301 KENRDGTGAGGKLIMTTARMSDSGRFMERANI--YRDSIRFGSERFDFNCK 350
           KENRDG     KL     R+SDSGRF+ER+N   Y D + F SERF    K
Sbjct: 301 KENRDGC----KLSQMERRISDSGRFIERSNASSYCDVLPFRSERFGLTYK 347

BLAST of MS012602 vs. TAIR 10
Match: AT4G40080.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 2.0e-91
Identity = 181/352 (51.42%), Postives = 242/352 (68.75%), Query Frame = 0

Query: 1   MVRTRKLSGLIGLIKDKASQSKAALIA---KPNILSFQLALLRATTHDPYAPPHDKHLAA 60
           M R    + LIG IKDKASQSKAAL++   K   LSF L++LRATTHDP  PP ++HLA 
Sbjct: 1   MGRITSFADLIGRIKDKASQSKAALVSSNTKSKTLSFHLSVLRATTHDPSTPPGNRHLAV 60

Query: 61  LLSLGKTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFT 120
           +LS G  SRATA++A+E +M+RL +T ++ VALK L+ +HHI+KHG FILQDQLSVFP +
Sbjct: 61  ILSAGTGSRATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHGRFILQDQLSVFPAS 120

Query: 121 GGRNYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKS 180
           GGRNYLKLS FRD  SP+ WELSSWVRWYA Y+E +LSTSRI+GFF++S+SS   +E+  
Sbjct: 121 GGRNYLKLSAFRDEKSPLMWELSSWVRWYALYLEHLLSTSRIMGFFISSTSSTIHKEEYE 180

Query: 181 EQISAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISV 240
           E +S++ NSDLLR+ ++LVGL+EE  K P         L D+I   V +DY+S++ E+  
Sbjct: 181 EMVSSLTNSDLLREIDALVGLLEEACKIPDLPFSGGKSLADKITQLVGEDYVSSINELYT 240

Query: 241 RVTEFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEI-LMDGFWGLIWETKNL 300
           R  EF +R + LSFG+++ELVC LKRLE CKE+ S      ++   +DGFWGL+ E K +
Sbjct: 241 RFNEFKERSNTLSFGDTIELVCALKRLESCKERLSEICHGNWKRGWIDGFWGLVLEVKGI 300

Query: 301 IGETKENRDGTGAGGKLIMTTARMS---DSGRFMERANI-YRDSIRFGSERF 345
           IG  ++N    G   K I+   +     +S RF +R  I Y + +RF S RF
Sbjct: 301 IGNLEDN---YGQIEKSIVGFGKRDKGYESARFTDRLIIGYSNPVRFSSGRF 349

BLAST of MS012602 vs. TAIR 10
Match: AT5G65370.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 138.7 bits (348), Expect = 9.6e-33
Identity = 95/301 (31.56%), Postives = 162/301 (53.82%), Query Frame = 0

Query: 6   KLSGLIGLIKDKASQSK---AALIAKPNILSFQLALLRATTHDPYAPPHDKHLAALLSLG 65
           KL+ L G++KD+ASQ K     L +  N  +  LALL+AT+H    PP DK++  L S  
Sbjct: 3   KLATLNGILKDEASQMKLNVVHLCSSVNAKTIDLALLKATSHTSNNPPSDKYVTFLQSTI 62

Query: 66  KTSRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILK-HGGFILQDQL------SVFP 125
            T        ++ ++ RL+ T +  VA KCL+ +H ++K   G+  +D L          
Sbjct: 63  DT--CYGPDTVDAILHRLRVTTDVCVAAKCLILLHKMVKSESGYNGEDSLRNNINHRTLI 122

Query: 126 FTGGRNYLKLSDFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREK 185
           +T G + LKL+D   NSS  + EL+ WV+WY QY++  LS + +LG         E++  
Sbjct: 123 YTQGGSNLKLNDLNVNSSRFTRELTPWVQWYKQYLDCYLSIAEVLGITPNIKEKNEDKRL 182

Query: 186 KSEQISAVFNSDLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEI 245
           +++++S+     +L+  + LV L E  + +P +     NK+V  +   +  DY SA++ +
Sbjct: 183 ETQRVSSYPMDCILKQIDFLVELFEHISDRPKAPQSKLNKIVIEMTELMVQDYFSAIRLM 242

Query: 246 SVRVTEFHQRLSCLSFGESVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKN 297
            +R  E + R++     +  ELV VL++LE+CKE  S   S + + L+  FW L+ + K+
Sbjct: 243 RIRFEELNVRVA-----KPNELVPVLEKLENCKEGLS-EFSWRSKYLIADFWYLVSKLKD 295

BLAST of MS012602 vs. TAIR 10
Match: AT5G10410.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 137.9 bits (346), Expect = 1.6e-32
Identity = 99/318 (31.13%), Postives = 163/318 (51.26%), Query Frame = 0

Query: 10  LIGLIKDKASQSKAALI---AKPNILSFQLALLRATTHDPYAPPHDKHLAALLSLGKTSR 69
           +IG  KDKAS  KA L+       +    LALL++TT  P  PP+  +++A++S   ++ 
Sbjct: 8   IIGKFKDKASIGKARLVHSFGSTAVKYIHLALLKSTTRTPNKPPNSDYVSAVISY--SNS 67

Query: 70  ATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGRNYLKLS 129
             A AA    + RL+ T N+ VA K L+ +H ++K      +D+        GRN LKL+
Sbjct: 68  RYAPAAFSAALWRLRVTKNAIVATKSLIVIHKLIKSS----RDKFE--GLGHGRNNLKLN 127

Query: 130 DFRDNSSPISWELSSWVRWYAQYIETVLSTSRILGFFVASSSSIEEREKKSEQISAVFNS 189
           +F D SS ++ ELS W+RWY QY++ +    ++LG F     + +++ ++ +++S+    
Sbjct: 128 EFSDKSSNLTLELSQWIRWYGQYLDRLSWVPKVLGSFPNLLVNPKDKVEEKDRVSSYQTG 187

Query: 190 DLLRDTESLVGLIEETTKKPHSLHLNANKLVDRILGFVADDYLSAMKEISVRVTEFHQRL 249
            ++R T+SLV   E    +P    +  NK+VD I   V +DY   ++ + VR+    +RL
Sbjct: 188 YIIRQTDSLVSFFEHICTRPEIPPMFQNKIVDEIRELVIEDYFKIVRLVMVRLQVLFERL 247

Query: 250 ---SCLSFGE--SVELVCVLKRLEDCKEKQSLGISAKYEILMDGFWGLIWETKNLIGETK 309
                   G+    +   +L RL +CKE  S G+  +   L D FW L+ E      E K
Sbjct: 248 IKPGVKPIGDLGLNDFSLLLVRLVECKESLS-GLFWRCRRLADDFWCLV-EMLKAETEKK 307

Query: 310 ENRDGTGAGGKLIMTTAR 320
            N+      G L+ TT +
Sbjct: 308 NNKQMIELAG-LVQTTVK 314

BLAST of MS012602 vs. TAIR 10
Match: AT2G01600.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 4.3e-17
Identity = 55/154 (35.71%), Postives = 84/154 (54.55%), Query Frame = 0

Query: 12  GLIKDKASQSKAALI-AKPNILSFQLALLRATTHDPYAPPHDKHLAALLSLGKTSRATA- 71
           G +KD    +K  L+          +A+++AT H    PP D+HL  + +    +RA A 
Sbjct: 12  GALKD---STKVGLVRVNSEYADLDVAIVKATNH-VECPPKDRHLRKIFAATSVTRARAD 71

Query: 72  -AAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGRNYLKLSDF 131
            A  I  L  RL  T N  VALK L+ +H +L+ G    +++L  F   G    L+LS+F
Sbjct: 72  VAYCIHALSRRLHKTRNWTVALKTLIVIHRLLREGDPTFREELLNFSQRG--RILQLSNF 131

Query: 132 RDNSSPISWELSSWVRWYAQYIETVLSTSRILGF 163
           +D+SSPI+W+ S+WVR YA ++E  L   R+L +
Sbjct: 132 KDDSSPIAWDCSAWVRTYALFLEERLECFRVLKY 159

BLAST of MS012602 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 6.2e-16
Identity = 52/147 (35.37%), Postives = 81/147 (55.10%), Query Frame = 0

Query: 6   KLSGLIGLIKDKASQSKAALIAKPNILS-FQLALLRATTHDPYAPPHDKHLAALLSLGKT 65
           KL   IG +KD+ S   A +  + + L+  ++A+++AT HD Y P  DK++  +L L   
Sbjct: 5   KLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDY-PAEDKYIREILCLTSY 64

Query: 66  SRATAAAAIEVLMDRLQSTHNSAVALKCLLAVHHILKHGGFILQDQLSVFPFTGGRNYLK 125
           SR   +A +  L  RL  T N +VALK L+ +  +L  G    + ++  F    G   L 
Sbjct: 65  SRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEI-FFATRRGTRLLN 124

Query: 126 LSDFRDNSSPISWELSSWVRWYAQYIE 152
           +SDFRD S   SW+ S++VR YA Y++
Sbjct: 125 MSDFRDASQSDSWDYSAFVRTYALYLD 149

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136401.11.0e-190100.00putative clathrin assembly protein At4g40080 [Momordica charantia][more]
XP_038903242.11.2e-15979.95putative clathrin assembly protein At4g40080 [Benincasa hispida][more]
KAG7015410.16.2e-15177.53putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_023552000.18.1e-15177.26putative clathrin assembly protein At4g40080 [Cucurbita pepo subsp. pepo][more]
XP_022929539.11.4e-15077.53putative clathrin assembly protein At4g40080 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8L9362.9e-9051.42Putative clathrin assembly protein At4g40080 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9FKQ21.3e-3131.56Putative clathrin assembly protein At5g65370 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8H0W92.3e-3131.13Putative clathrin assembly protein At5g10410 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8LBH26.1e-1635.71Putative clathrin assembly protein At2g01600 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8GX478.8e-1535.37Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1C3E85.1e-191100.00putative clathrin assembly protein At4g40080 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1EP166.7e-15177.53putative clathrin assembly protein At4g40080 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JCT45.3e-14876.44putative clathrin assembly protein At4g40080 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A1S3C1C01.0e-14677.78putative clathrin assembly protein At4g40080 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A5A7TT501.3e-14677.21Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
AT4G40080.12.0e-9151.42ENTH/ANTH/VHS superfamily protein [more]
AT5G65370.19.6e-3331.56ENTH/ANTH/VHS superfamily protein [more]
AT5G10410.11.6e-3231.13ENTH/ANTH/VHS superfamily protein [more]
AT2G01600.14.3e-1735.71ENTH/ANTH/VHS superfamily protein [more]
AT4G02650.16.2e-1635.37ENTH/ANTH/VHS superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013809ENTH domainSMARTSM00273enth_2coord: 32..164
e-value: 1.7E-9
score: 47.5
IPR013809ENTH domainPROSITEPS50942ENTHcoord: 26..164
score: 17.396008
IPR008942ENTH/VHSGENE3D1.25.40.90coord: 7..163
e-value: 1.8E-31
score: 110.8
IPR008942ENTH/VHSSUPERFAMILY48464ENTH/VHS domaincoord: 34..162
IPR011417AP180 N-terminal homology (ANTH) domainPFAMPF07651ANTHcoord: 36..263
e-value: 2.4E-18
score: 66.1
NoneNo IPR availablePANTHERPTHR22951CLATHRIN ASSEMBLY PROTEINcoord: 5..347
NoneNo IPR availablePANTHERPTHR22951:SF76OS09G0468150 PROTEINcoord: 5..347
NoneNo IPR availableCDDcd16987ANTH_N_AP180_plantcoord: 34..157
e-value: 7.19846E-52
score: 166.645

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS012602.1MS012602.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005543 phospholipid binding