MS012325 (gene) Bitter gourd (TR) v1

BLAST of MS012325 vs. NCBI nr
Match: XP_022149149.1 (protein DMR6-LIKE OXYGENASE 1-like [Momordica charantia])

HSP 1 Score: 701.8 bits (1810), Expect = 2.9e-198
Identity = 343/346 (99.13%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   PAMASVKTIAQTPNLTSIPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAE 60
           PAMASVKTIAQTPNLTSIPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAE
Sbjct: 28  PAMASVKTIAQTPNLTSIPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAE 87

Query: 61  VVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYG 120
           VVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYG
Sbjct: 88  VVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYG 147

Query: 121 TSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARGISESL 180
           TSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARGISESL
Sbjct: 148 TSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARGISESL 207

Query: 181 GLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQIL 240
           GLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQN+ICGLQIL
Sbjct: 208 GLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNKICGLQIL 267

Query: 241 HQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDTL 300
           HQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAI+NKKATRMSVGIAIGPSLDT+
Sbjct: 268 HQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAIVNKKATRMSVGIAIGPSLDTV 327

Query: 301 VGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRLV 347
           VGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRLV
Sbjct: 328 VGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRLV 373

BLAST of MS012325 vs. NCBI nr
Match: XP_022141027.1 (protein DMR6-LIKE OXYGENASE 1-like isoform X1 [Momordica charantia] >XP_022159285.1 protein DMR6-LIKE OXYGENASE 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 537.7 bits (1384), Expect = 7.2e-149
Identity = 255/346 (73.70%), Postives = 300/346 (86.71%), Query Frame = 0

Query: 1   PAMASVKTIAQTPNLTSIPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAE 60
           PA+ASVKTIA+TPNL SIPSSY+FSA+ GA  AAP+GVEDSIPT+DFSLLTMGTPDQRA+
Sbjct: 4   PAVASVKTIAETPNLASIPSSYIFSANDGAAKAAPRGVEDSIPTIDFSLLTMGTPDQRAK 63

Query: 61  VVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYG 120
           VVD+LGKACQDWGFF+V+NHGV E VM EM+DICREFFDL EEEK EY+T+HVLDPIRYG
Sbjct: 64  VVDELGKACQDWGFFVVMNHGVEERVMREMIDICREFFDLTEEEKTEYKTEHVLDPIRYG 123

Query: 121 TSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARGISESL 180
           TSFNP+ EKV FWRDYLKI VHP FH+PTKP RFREI EEYC+R  E+AREL RGISESL
Sbjct: 124 TSFNPQKEKVLFWRDYLKIFVHPKFHSPTKPPRFREILEEYCKRSIEMARELVRGISESL 183

Query: 181 GLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQIL 240
           GLE C LE+AV+ +SCS +   N YPP PQ E+A GLP HSD CLLT +LQNQI GLQIL
Sbjct: 184 GLETCHLERAVDFESCSTLFAANFYPPYPQPELARGLPSHSDQCLLTLLLQNQISGLQIL 243

Query: 241 HQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDTL 300
           HQG WV+VNPIPNSFLVN ADQLEI SNGKYKS+LHRA++N KATR+S+ +A+G S +T+
Sbjct: 244 HQGNWVDVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNNKATRLSIAVAVGSSPETV 303

Query: 301 VGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRLV 347
           V PAP+L+++LTNPPL+KHIKY DYM++VQ+++LQ  S LD +RL+
Sbjct: 304 VSPAPQLLNKLTNPPLFKHIKYTDYMQMVQSSNLQ-DSCLDRIRLL 348

BLAST of MS012325 vs. NCBI nr
Match: XP_038904929.1 (protein DMR6-LIKE OXYGENASE 2-like [Benincasa hispida])

HSP 1 Score: 526.9 bits (1356), Expect = 1.3e-145
Identity = 250/347 (72.05%), Postives = 297/347 (85.59%), Query Frame = 0

Query: 2   AMASVKTIAQTPNLTSIPSSYVFSA--DGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRA 61
           AMASVK++A TPNL S+PSS++F+   D  +V A  QG EDSIP +D SLL  GTP QRA
Sbjct: 2   AMASVKSLADTPNLASVPSSFMFATDDDSDSVTAVSQGAEDSIPIIDTSLLIDGTPHQRA 61

Query: 62  EVVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRY 121
           +V+++LGKAC+DWGFFMV+NHGV E +M EMV+IC+EFFDL+EEEKREYETK VLDPIRY
Sbjct: 62  KVINELGKACEDWGFFMVVNHGVGERLMREMVEICKEFFDLKEEEKREYETKSVLDPIRY 121

Query: 122 GTSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARGISES 181
           GTSFNPK+EKVFFWRDYLKIMVHPNFH+PTKP+RFREI EEYC+R+RE+ REL RGISES
Sbjct: 122 GTSFNPKVEKVFFWRDYLKIMVHPNFHSPTKPTRFREILEEYCKRIREMTRELVRGISES 181

Query: 182 LGLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQI 241
           LGLE C L+KA NL+SCS +   NLYPPCPQ E+A GLP HSDHCLLT +LQNQI GLQI
Sbjct: 182 LGLEECCLDKAANLESCSIVFAANLYPPCPQPELARGLPSHSDHCLLTILLQNQIAGLQI 241

Query: 242 LHQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDT 301
           LH  KW+NVNPIPNS LVN ADQLEI SNGKYKS+LHRAI+N KATR+S+ +A+GPSL+T
Sbjct: 242 LHHDKWLNVNPIPNSLLVNVADQLEILSNGKYKSVLHRAIVNDKATRISIAMAVGPSLET 301

Query: 302 LVGPAPELVHQLT-NPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRL 346
           +VGPAP+L+++ T NPPL+K+IKY DYM +VQ+N LQ KS LD +RL
Sbjct: 302 VVGPAPQLINKHTNNPPLFKNIKYIDYMGIVQSNKLQGKSTLDRIRL 348

BLAST of MS012325 vs. NCBI nr
Match: XP_023552481.1 (protein DMR6-LIKE OXYGENASE 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 514.6 bits (1324), Expect = 6.6e-142
Identity = 242/351 (68.95%), Postives = 290/351 (82.62%), Query Frame = 0

Query: 2   AMASVKTIAQTPNLTSIPSSYVFSADGGA------VDAAPQGVEDSIPTVDFSLLTMGTP 61
           A+ SVK IA+TPNL SIPSSY+F+    +       DAAP GVEDSIPT+DFSLLT GTP
Sbjct: 28  ALPSVKAIAETPNLASIPSSYIFTTSDDSDDVATTADAAPHGVEDSIPTIDFSLLTTGTP 87

Query: 62  DQRAEVVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLD 121
            QR++VV++LGKAC DWGFFMVINHGV E +M EMV+ICREFFDL EEEKR YETKHVLD
Sbjct: 88  HQRSKVVNELGKACHDWGFFMVINHGVGEGLMKEMVEICREFFDLTEEEKRVYETKHVLD 147

Query: 122 PIRYGTSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARG 181
           PIRYGTSFNPKME+VFFWRDYLK++VHP FH+P KP+RFREI EEY +R++E+AREL RG
Sbjct: 148 PIRYGTSFNPKMEEVFFWRDYLKVLVHPKFHSPPKPTRFREILEEYSKRIKEMARELVRG 207

Query: 182 ISESLGLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQIC 241
           ISESLGLE C +EKA +L+S S +   NLYPPCPQ ++A GLPPHSD CLLT +LQN + 
Sbjct: 208 ISESLGLEACNIEKAADLESSSTLFAANLYPPCPQPQLARGLPPHSDQCLLTVLLQNHVA 267

Query: 242 GLQILHQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGP 301
           GLQILH  +W+ VNPIPN+ LVNTADQLEI SNGKYKS+LHRA++N KATR+S+ +AIGP
Sbjct: 268 GLQILHDHQWLTVNPIPNALLVNTADQLEIMSNGKYKSVLHRAMVNDKATRISIAMAIGP 327

Query: 302 SLDTLVGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRLV 347
           S  TLV P PEL+H+  +PPL+K I YKDYME++Q+N L+ KS LD +RL+
Sbjct: 328 SSQTLVAPLPELLHKHNSPPLFKSIMYKDYMEMLQSNKLERKSCLDRIRLL 378

BLAST of MS012325 vs. NCBI nr
Match: KAG6576847.1 (2-oxoglutarate-dependent dioxygenase 19, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 513.8 bits (1322), Expect = 1.1e-141
Identity = 243/351 (69.23%), Postives = 289/351 (82.34%), Query Frame = 0

Query: 2   AMASVKTIAQTPNLTSIPSSYVFSADGGA------VDAAPQGVEDSIPTVDFSLLTMGTP 61
           A+ SVK IA+TPNL SIPSSY+F+    +       DAAP  VEDSIPT+DFSLLT GTP
Sbjct: 2   ALPSVKAIAETPNLASIPSSYIFTTSDDSDDVATTADAAPHSVEDSIPTIDFSLLTTGTP 61

Query: 62  DQRAEVVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLD 121
            QR++VVD+LGKAC DWGFFMVINHGV E +M EMV+ICREFFDL EEEKR YETKHVLD
Sbjct: 62  HQRSKVVDELGKACHDWGFFMVINHGVGEGLMKEMVEICREFFDLTEEEKRVYETKHVLD 121

Query: 122 PIRYGTSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARG 181
           PIRYGTSFNPKME+VFFWRDYLK++VHP FH+P+KP+RFREI EEY +R++E+AREL RG
Sbjct: 122 PIRYGTSFNPKMEEVFFWRDYLKVLVHPKFHSPSKPTRFREILEEYSKRIKEMARELVRG 181

Query: 182 ISESLGLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQIC 241
           ISESLGLE C LEKA +L+S S +   NLYPPCPQ ++A GLPPHSD CLLT +LQN + 
Sbjct: 182 ISESLGLEACNLEKAADLESSSTLFAANLYPPCPQPQLARGLPPHSDQCLLTVLLQNHVA 241

Query: 242 GLQILHQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGP 301
           GLQILH  +W+ VNP+PN  LVNTADQLEI SNGKYKS+LHRA++N KATR+S+ +AIGP
Sbjct: 242 GLQILHDHQWLTVNPVPNVLLVNTADQLEIMSNGKYKSVLHRAMVNDKATRISIAMAIGP 301

Query: 302 SLDTLVGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRLV 347
           S  TLV P PEL+H+  +PPL+K I YKDYME++Q+N L+ KS LD VRL+
Sbjct: 302 SSQTLVAPLPELLHKHNSPPLFKSIMYKDYMEMLQSNKLEGKSCLDRVRLL 352

BLAST of MS012325 vs. ExPASy Swiss-Prot
Match: Q6Z244 (2-oxoglutarate-dependent dioxygenase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD19 PE=1 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 2.0e-80
Identity = 153/323 (47.37%), Postives = 205/323 (63.47%), Query Frame = 0

Query: 25  SADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAEVVDQLGKACQDWGFFMVINHGVAE 84
           +ADG A  +        IP VD  +L  G  D+R+  +  LG+AC+DWGFFMV NHGV E
Sbjct: 18  AADGSATPS------QGIPVVDLGVLINGAADERSRAIRDLGRACEDWGFFMVTNHGVPE 77

Query: 85  TVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYGTSFNPKMEKVFFWRDYLKIMVHPN 144
            +   ++D C+E F L  EEK+EY     +DPIR GT F   ++ V   RDYLK+  HP 
Sbjct: 78  ALREAIMDACKELFRLPLEEKKEYMRAKPMDPIRIGTGFYSVVDAVPCRRDYLKMFSHPE 137

Query: 145 FHAPTKPSRFREISEEYCRRVRELARELARGISESLGLEGCTLEKAVNLKSCSQILVGNL 204
           FH P KP++ REI+ EY    R L  EL + ISESLGL G  L +A+NL+SC QILVGN 
Sbjct: 138 FHCPEKPAKLREIATEYATCTRALLLELTKAISESLGLAGGRLSEALNLESCFQILVGNH 197

Query: 205 YPPCPQA-EVAMGLPPHSDHCLLTFILQNQICGLQILHQGKWVNVNPIPNSFLVNTADQL 264
           YP C +  E AMGL  HSDH LLT + QN + GLQ+ H G+W+   P+P SF V   DQL
Sbjct: 198 YPACSRPDEQAMGLSAHSDHGLLTLLFQNGVDGLQVKHDGEWLLAKPLPGSFFVIAGDQL 257

Query: 265 EIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDTLVGPAPELVHQLTNPPLYKHIKYK 324
           EI +NG+YK +LHRA++  + +RMS    IGP +DT+V P PE+         ++ I+Y+
Sbjct: 258 EIVTNGRYKGVLHRAVVGGEQSRMSFVSLIGPCMDTVVEPLPEMAADGRGLE-FRGIRYR 317

Query: 325 DYMELVQTNDLQHKSMLDLVRLV 347
           DYME+ Q+N +  K+ LD+VR++
Sbjct: 318 DYMEMQQSNSINEKTALDIVRVM 333

BLAST of MS012325 vs. ExPASy Swiss-Prot
Match: Q6YYX9 (Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC1 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.3e-60
Identity = 126/339 (37.17%), Postives = 193/339 (56.93%), Query Frame = 0

Query: 15  LTSIPSSYVFSADG----GAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAEVVDQLGKACQ 74
           +T +P +YV  A       A  AA  G    +P VD S L +  P +R  V+  L  AC+
Sbjct: 49  ITRLPGNYVLPASDRPGQAAGAAAAAGGSVKLPVVDLSRLRV--PSERGAVLRTLDAACR 108

Query: 75  DWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYGTSFNPKMEKV 134
           ++GFF V+NHGV   V+  M+D+ R FF+L + E+  Y +  V  P+RYGTSFN   + V
Sbjct: 109 EYGFFQVVNHGVGGEVVGGMLDVARRFFELPQPERERYMSADVRAPVRYGTSFNQVRDAV 168

Query: 135 FFWRDYLKIMVHPNFHA----PTKPSRFREISEEYCRRVRELARELARGISESLGLEGCT 194
             WRD+LK+   P        PT P+  RE++  Y    + +  E+     E+LG+ G  
Sbjct: 169 LCWRDFLKLACMPLAAVVESWPTSPADLREVASRYAEANQRVFMEVMEAALEALGVGGGG 228

Query: 195 LEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQILHQGKWV 254
           + +  +L + +Q++  N YP CPQ E+ +G+PPHSD+  LT +LQ+++ GLQ++H G+W+
Sbjct: 229 VME--DLAAGTQMMTVNCYPECPQPELTLGMPPHSDYGFLTLVLQDEVAGLQVMHAGEWL 288

Query: 255 NVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDTLVGPAPE 314
            V+P+P SF+VN  D LEI SNG+Y+S+LHR  +N +  R+SV      + + +V PAPE
Sbjct: 289 TVDPLPGSFVVNVGDHLEILSNGRYRSVLHRVKVNSRRLRVSVASFHSVAPERVVSPAPE 348

Query: 315 LVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRL 346
           L+    +P  Y       ++  + +    HKS L   RL
Sbjct: 349 LIDD-RHPRRYMDTDLATFLAYLASAAGNHKSFLHSRRL 382

BLAST of MS012325 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.5e-59
Identity = 128/328 (39.02%), Postives = 186/328 (56.71%), Query Frame = 0

Query: 18  IPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAEVVDQLGKACQDWGFFMV 77
           IPS+YV         +  +   DSIP +D  L  +  P+ RA +V QL  AC  +GFF +
Sbjct: 20  IPSNYVRPISDRPNLSEVESSGDSIPLID--LRDLHGPN-RAVIVQQLASACSTYGFFQI 79

Query: 78  INHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYGTSFNPKMEKVFFWRDYL 137
            NHGV +T + +M  + REFF   E E+ ++ +       R  TSFN   +KV  WRD+L
Sbjct: 80  KNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFL 139

Query: 138 KIMVHP--NF--HAPTKPSRFREISEEYCRRVRELARELARGISESLGLEGCTLEKAVNL 197
           ++   P  +F    P+ P  FRE++ EY   VR L   L   ISESLGLE   +     L
Sbjct: 140 RLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHISNI--L 199

Query: 198 KSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQILHQGKWVNVNPIPN 257
              +Q +  N YPPCP+ E+  GLP H D  ++T +LQ+Q+ GLQ+    KWV V+PIPN
Sbjct: 200 GKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPN 259

Query: 258 SFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDTLVGPAPELVHQLTN 317
           +F+VN  DQ+++ SN KYKS+LHRA++N +  R+S+     PS D ++GPA ELV++  +
Sbjct: 260 TFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDS 319

Query: 318 PPLYKHIKYKDYMELVQTNDLQHKSMLD 342
             +Y+   + +Y +      L   S LD
Sbjct: 320 LAIYRTYPFVEYWDKFWNRSLATASCLD 342

BLAST of MS012325 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.0e-57
Identity = 124/329 (37.69%), Postives = 185/329 (56.23%), Query Frame = 0

Query: 18  IPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAEVVDQLGKACQDWGFFMV 77
           +PS+YV         +  Q   DSIP +D  L  +  P+ RA++++Q   AC   GFF +
Sbjct: 18  VPSNYVRPVSDRPKMSEVQTSGDSIPLID--LHDLHGPN-RADIINQFAHACSSCGFFQI 77

Query: 78  INHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYGTSFNPKMEKVFFWRDYL 137
            NHGV E  + +M++  REFF   E E+ ++ +       R  TSFN   EKV  WRD+L
Sbjct: 78  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFL 137

Query: 138 KIMVHP--NF--HAPTKPSRFREISEEYCRRVRELARELARGISESLGLEGCTLEKAVNL 197
           ++  +P  +F    P+ P  FRE++ EY   VR L   L   ISESLGL    +   +  
Sbjct: 138 RLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG- 197

Query: 198 KSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQILHQGKWVNVNPIPN 257
               Q +  N YP CPQ E+  GLP H D  L+T +LQ+++ GLQ+   GKW+ VNP+PN
Sbjct: 198 -KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPN 257

Query: 258 SFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDTLVGPAPELVH-QLT 317
           +F+VN  DQ+++ SN KYKS+LHRA++N    R+S+     PS D ++ PA EL++ +  
Sbjct: 258 TFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEED 317

Query: 318 NPPLYKHIKYKDYMELVQTNDLQHKSMLD 342
           +P +Y++  Y +Y E         +S +D
Sbjct: 318 SPAIYRNFTYAEYFEKFWDTAFDTESCID 341

BLAST of MS012325 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 4.4e-56
Identity = 122/308 (39.61%), Postives = 176/308 (57.14%), Query Frame = 0

Query: 42  IPTVDFSLLTMGTPDQRAEVVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLE 101
           IP VD     + +PD RA VV  +G AC+  GFF V+NHG+   ++  ++++ REFF L 
Sbjct: 43  IPVVD-----LASPD-RAAVVSAVGDACRTHGFFQVVNHGIDAALIASVMEVGREFFRLP 102

Query: 102 EEEKREYETKHVLDPIRYGTSFNPKMEKVFFWRDYLKIMVHPNFHA----PTKPSRFREI 161
            EEK +  +      IR  TSFN + E V  WRDYL++  +P        P+ P  F+EI
Sbjct: 103 AEEKAKLYSDDPAKKIRLSTSFNVRKETVHNWRDYLRLHCYPLHQFVPDWPSNPPSFKEI 162

Query: 162 SEEYCRRVRELARELARGISESLGLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGL 221
              YC  VREL   L   ISESLGLEG  + +   L    Q +  N YP CP+ E+  GL
Sbjct: 163 IGTYCTEVRELGFRLYEAISESLGLEGGYMRE--TLGEQEQHMAVNYYPQCPEPELTYGL 222

Query: 222 PPHSDHCLLTFIL-QNQICGLQILHQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLH 281
           P H+D   LT +L  +Q+ GLQ+L+ GKW+ VNP P + ++N  DQL+  SNGKY+S+ H
Sbjct: 223 PAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVINIGDQLQALSNGKYRSVWH 282

Query: 282 RAILNKKATRMSVGIAIGPSLDTLVGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQH 341
           RA++N    RMSV   + P     +GPA +L+    +P +Y++  Y +Y +   + +L  
Sbjct: 283 RAVVNSDRERMSVASFLCPCNSVELGPAKKLITD-DSPAVYRNYTYDEYYKKFWSRNLDQ 341

Query: 342 KSMLDLVR 345
           +  L+L R
Sbjct: 343 EHCLELFR 341

BLAST of MS012325 vs. ExPASy TrEMBL
Match: A0A6J1D661 (protein DMR6-LIKE OXYGENASE 1-like OS=Momordica charantia OX=3673 GN=LOC111017638 PE=3 SV=1)

HSP 1 Score: 701.8 bits (1810), Expect = 1.4e-198
Identity = 343/346 (99.13%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   PAMASVKTIAQTPNLTSIPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAE 60
           PAMASVKTIAQTPNLTSIPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAE
Sbjct: 28  PAMASVKTIAQTPNLTSIPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAE 87

Query: 61  VVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYG 120
           VVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYG
Sbjct: 88  VVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYG 147

Query: 121 TSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARGISESL 180
           TSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARGISESL
Sbjct: 148 TSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARGISESL 207

Query: 181 GLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQIL 240
           GLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQN+ICGLQIL
Sbjct: 208 GLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNKICGLQIL 267

Query: 241 HQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDTL 300
           HQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAI+NKKATRMSVGIAIGPSLDT+
Sbjct: 268 HQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAIVNKKATRMSVGIAIGPSLDTV 327

Query: 301 VGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRLV 347
           VGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRLV
Sbjct: 328 VGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRLV 373

BLAST of MS012325 vs. ExPASy TrEMBL
Match: A0A6J1CIQ0 (protein DMR6-LIKE OXYGENASE 1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011533 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.5e-149
Identity = 255/346 (73.70%), Postives = 300/346 (86.71%), Query Frame = 0

Query: 1   PAMASVKTIAQTPNLTSIPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAE 60
           PA+ASVKTIA+TPNL SIPSSY+FSA+ GA  AAP+GVEDSIPT+DFSLLTMGTPDQRA+
Sbjct: 4   PAVASVKTIAETPNLASIPSSYIFSANDGAAKAAPRGVEDSIPTIDFSLLTMGTPDQRAK 63

Query: 61  VVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYG 120
           VVD+LGKACQDWGFF+V+NHGV E VM EM+DICREFFDL EEEK EY+T+HVLDPIRYG
Sbjct: 64  VVDELGKACQDWGFFVVMNHGVEERVMREMIDICREFFDLTEEEKTEYKTEHVLDPIRYG 123

Query: 121 TSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARGISESL 180
           TSFNP+ EKV FWRDYLKI VHP FH+PTKP RFREI EEYC+R  E+AREL RGISESL
Sbjct: 124 TSFNPQKEKVLFWRDYLKIFVHPKFHSPTKPPRFREILEEYCKRSIEMARELVRGISESL 183

Query: 181 GLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQIL 240
           GLE C LE+AV+ +SCS +   N YPP PQ E+A GLP HSD CLLT +LQNQI GLQIL
Sbjct: 184 GLETCHLERAVDFESCSTLFAANFYPPYPQPELARGLPSHSDQCLLTLLLQNQISGLQIL 243

Query: 241 HQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDTL 300
           HQG WV+VNPIPNSFLVN ADQLEI SNGKYKS+LHRA++N KATR+S+ +A+G S +T+
Sbjct: 244 HQGNWVDVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNNKATRLSIAVAVGSSPETV 303

Query: 301 VGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRLV 347
           V PAP+L+++LTNPPL+KHIKY DYM++VQ+++LQ  S LD +RL+
Sbjct: 304 VSPAPQLLNKLTNPPLFKHIKYTDYMQMVQSSNLQ-DSCLDRIRLL 348

BLAST of MS012325 vs. ExPASy TrEMBL
Match: A0A6J1JCE3 (protein DMR6-LIKE OXYGENASE 2-like OS=Cucurbita maxima OX=3661 GN=LOC111483195 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.3e-140
Identity = 243/351 (69.23%), Postives = 287/351 (81.77%), Query Frame = 0

Query: 2   AMASVKTIAQTPNLTSIPSSYVFSAD------GGAVDAAPQGVEDSIPTVDFSLLTMGTP 61
           A+ SVK IA+TPNL SIPSSY+F+          A DAAP  VE SIPT+DFSLLT GT 
Sbjct: 33  ALPSVKAIAETPNLASIPSSYIFTTSDDSDDVAAAADAAPHRVEVSIPTIDFSLLTTGTS 92

Query: 62  DQRAEVVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLD 121
            QR++VV++LGKAC DWGFFMVINHGV E +M EMV+ICREFFDL+EEEKREYETKHVLD
Sbjct: 93  HQRSKVVNELGKACHDWGFFMVINHGVGEGLMKEMVEICREFFDLKEEEKREYETKHVLD 152

Query: 122 PIRYGTSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARG 181
           PIRYGTSFNPKME+VFFWRDYLK++VHP FH+P KP+RFREI EEY +R+RE+AREL RG
Sbjct: 153 PIRYGTSFNPKMEEVFFWRDYLKVLVHPKFHSPPKPTRFREILEEYSKRIREMARELVRG 212

Query: 182 ISESLGLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQIC 241
           ISESLGLE   LEKA  L+S S +   NLYPPCPQ + A GLPPHSD CLLT +LQN + 
Sbjct: 213 ISESLGLEAYNLEKAAELESSSTLFAANLYPPCPQPQFARGLPPHSDQCLLTVLLQNHVS 272

Query: 242 GLQILHQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGP 301
           GLQILH  +W+ VNP+PN+ LVNTADQLEI SNGKYKS+LHRA++N KATR+S+ +AIGP
Sbjct: 273 GLQILHDHQWLTVNPVPNALLVNTADQLEIMSNGKYKSVLHRAMVNDKATRISIAMAIGP 332

Query: 302 SLDTLVGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRLV 347
           S  TLV P PEL+H+  NPPL+K IKYKDYME++Q+N L+ KS LD +RL+
Sbjct: 333 SSQTLVAPLPELLHKHNNPPLFKTIKYKDYMEMLQSNKLERKSCLDRLRLL 383

BLAST of MS012325 vs. ExPASy TrEMBL
Match: A0A1S3CJI6 (protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo OX=3656 GN=LOC103501162 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.5e-139
Identity = 241/348 (69.25%), Postives = 287/348 (82.47%), Query Frame = 0

Query: 3   MASVKTIAQTPNLTSIPSSYVFSADGG----AVDAAPQGVEDSIPTVDFSLLTMGTPDQR 62
           MASVK+IA TPNLTSIPSS++F+ D        DA+PQG EDSIP +D SLL  GTP QR
Sbjct: 1   MASVKSIADTPNLTSIPSSFIFATDDSFDDVTADASPQGAEDSIPIIDLSLLINGTPQQR 60

Query: 63  AEVVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIR 122
           A+VV++LGKAC+DWGFFMV+NHGV E +M ++++IC EFF+L+EEEKREYETKHVLDPIR
Sbjct: 61  AKVVNELGKACEDWGFFMVVNHGVEEKLMKDLMEICIEFFELKEEEKREYETKHVLDPIR 120

Query: 123 YGTSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARGISE 182
           YGTSFNPK+EK FFWRDYLKI VHP FHAPTKP+RFR I EEYC RVRE  REL RGISE
Sbjct: 121 YGTSFNPKVEKAFFWRDYLKIKVHPKFHAPTKPTRFRGILEEYCTRVRETTRELVRGISE 180

Query: 183 SLGLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQ 242
           SLGLEGC LEKA +L+S   +   NLYPPCPQ E+A GLP HSD CLLT ++ N+I GLQ
Sbjct: 181 SLGLEGCFLEKATDLESSLILFAANLYPPCPQPELARGLPSHSDLCLLTILITNEIAGLQ 240

Query: 243 ILHQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLD 302
           ILH  KW NVNPIPNS ++N  DQLEI SNGKYKS+LHRA +N KATR+S+G+A+GPS +
Sbjct: 241 ILHHDKWFNVNPIPNSLIINVGDQLEILSNGKYKSVLHRAKVNDKATRISIGMAVGPSHE 300

Query: 303 TLVGPAPELVHQLT-NPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRL 346
           T+VGPAP+LV++ T NPP++K IKYKDYME++Q+N LQ KS+LD  RL
Sbjct: 301 TVVGPAPQLVNEHTNNPPMFKSIKYKDYMEIMQSNQLQGKSILDRFRL 348

BLAST of MS012325 vs. ExPASy TrEMBL
Match: A0A0A0L0R1 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G112650 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 3.3e-139
Identity = 242/348 (69.54%), Postives = 289/348 (83.05%), Query Frame = 0

Query: 3   MASVKTIAQTPNLTSIPSSYVFSADGG----AVDAAPQGVEDSIPTVDFSLLTMGTPDQR 62
           MASVK+IA +PNLTSIPSS++F+ D      A DA+ QG EDSIP +D SLL  GTP QR
Sbjct: 1   MASVKSIADSPNLTSIPSSFIFATDDSFDDVAADASLQGAEDSIPIIDLSLLINGTPQQR 60

Query: 63  AEVVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIR 122
           A+VV++LGKAC+DWGFFMV+NHGV E +M ++++IC EFF+L+EEEKREYETKHVLDPIR
Sbjct: 61  AKVVNELGKACEDWGFFMVVNHGVEEKLMKDLMEICVEFFELKEEEKREYETKHVLDPIR 120

Query: 123 YGTSFNPKMEKVFFWRDYLKIMVHPNFHAPTKPSRFREISEEYCRRVRELARELARGISE 182
           YGTSFNPKMEK FFWRDYLKIMVHP FHAPTKP+RFR I EEYC  VRE+ REL RGISE
Sbjct: 121 YGTSFNPKMEKAFFWRDYLKIMVHPKFHAPTKPTRFRGILEEYCTSVREMTRELLRGISE 180

Query: 183 SLGLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQ 242
           SLGLEGC LEKA +L+S   +   NLYPPCPQ E+A GLP HSD CLLT +L NQI GLQ
Sbjct: 181 SLGLEGCFLEKATDLESSLILFAANLYPPCPQPELARGLPSHSDLCLLTILLTNQIAGLQ 240

Query: 243 ILHQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLD 302
           ILH  KW NVNPIPNSF++N  DQLEI SNGKY+S+LHRA +N KATR+S+G+A+GPS +
Sbjct: 241 ILHHDKWFNVNPIPNSFIINVGDQLEILSNGKYESVLHRAKVNDKATRISIGMAVGPSHE 300

Query: 303 TLVGPAPELVHQLT-NPPLYKHIKYKDYMELVQTNDLQHKSMLDLVRL 346
           T+VGPAP+LV++ T NPP++K IKYKDYME++Q++ LQ KS+LD  RL
Sbjct: 301 TVVGPAPQLVNEDTNNPPMFKSIKYKDYMEIMQSSQLQEKSILDRFRL 348

BLAST of MS012325 vs. TAIR 10
Match: AT2G36690.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 4.8e-66
Identity = 127/336 (37.80%), Postives = 205/336 (61.01%), Query Frame = 0

Query: 15  LTSIPSSYVF-SADGGAVDAAPQGVEDS----IPTVDFSLLTMGTPDQRAEVVDQLGKAC 74
           LT +P+ Y++   D   +  + + ++ +    +P +DF+ L       R  V+  + +AC
Sbjct: 29  LTKVPTKYIWPEPDRPILTKSDKLIKPNKNLKLPLIDFAEL---LGPNRPHVLRTIAEAC 88

Query: 75  QDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYGTSFNPKMEK 134
           + +GFF V+NHG+   V   M+D+C+ FF+L  EE+ +Y +  +  P+RYGTSFN   + 
Sbjct: 89  KTYGFFQVVNHGMEGDVSKNMIDVCKRFFELPYEERSKYMSSDMSAPVRYGTSFNQIKDN 148

Query: 135 VFFWRDYLKIMVHP----NFHAPTKPSRFREISEEYCRRVRELARELARGISESLGLEGC 194
           VF WRD+LK+  HP      H P+ PS FR  +  Y +  +E+   + + I ESL ++G 
Sbjct: 149 VFCWRDFLKLYAHPLPDYLPHWPSSPSDFRSSAATYAKETKEMFEMMVKAILESLEIDGS 208

Query: 195 TLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQILHQGKW 254
             E A  L+  SQ++V N YPPCP+ E+ +G+PPHSD+  LT +LQ+++ GLQIL++ +W
Sbjct: 209 D-EAAKELEEGSQVVVVNCYPPCPEPELTLGMPPHSDYGFLTLLLQDEVEGLQILYRDEW 268

Query: 255 VNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDTLVGPAP 314
           V V+PIP SF+VN  D LEIFSNG+YKS+LHR ++N    R+SV       L ++V P+P
Sbjct: 269 VTVDPIPGSFVVNVGDHLEIFSNGRYKSVLHRVLVNSTKPRISVASLHSFPLTSVVKPSP 328

Query: 315 ELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKSMLD 342
           +LV +  NP  Y    +  +++ + + + + K+ L+
Sbjct: 329 KLVDK-HNPSQYMDTDFTTFLQYITSREPKWKNFLE 359

BLAST of MS012325 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 229.6 bits (584), Expect = 3.9e-60
Identity = 128/328 (39.02%), Postives = 186/328 (56.71%), Query Frame = 0

Query: 18  IPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAEVVDQLGKACQDWGFFMV 77
           IPS+YV         +  +   DSIP +D  L  +  P+ RA +V QL  AC  +GFF +
Sbjct: 20  IPSNYVRPISDRPNLSEVESSGDSIPLID--LRDLHGPN-RAVIVQQLASACSTYGFFQI 79

Query: 78  INHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYGTSFNPKMEKVFFWRDYL 137
            NHGV +T + +M  + REFF   E E+ ++ +       R  TSFN   +KV  WRD+L
Sbjct: 80  KNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFL 139

Query: 138 KIMVHP--NF--HAPTKPSRFREISEEYCRRVRELARELARGISESLGLEGCTLEKAVNL 197
           ++   P  +F    P+ P  FRE++ EY   VR L   L   ISESLGLE   +     L
Sbjct: 140 RLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHISNI--L 199

Query: 198 KSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQILHQGKWVNVNPIPN 257
              +Q +  N YPPCP+ E+  GLP H D  ++T +LQ+Q+ GLQ+    KWV V+PIPN
Sbjct: 200 GKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPN 259

Query: 258 SFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDTLVGPAPELVHQLTN 317
           +F+VN  DQ+++ SN KYKS+LHRA++N +  R+S+     PS D ++GPA ELV++  +
Sbjct: 260 TFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDS 319

Query: 318 PPLYKHIKYKDYMELVQTNDLQHKSMLD 342
             +Y+   + +Y +      L   S LD
Sbjct: 320 LAIYRTYPFVEYWDKFWNRSLATASCLD 342

BLAST of MS012325 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 225.3 bits (573), Expect = 7.4e-59
Identity = 124/329 (37.69%), Postives = 185/329 (56.23%), Query Frame = 0

Query: 18  IPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAEVVDQLGKACQDWGFFMV 77
           +PS+YV         +  Q   DSIP +D  L  +  P+ RA++++Q   AC   GFF +
Sbjct: 18  VPSNYVRPVSDRPKMSEVQTSGDSIPLID--LHDLHGPN-RADIINQFAHACSSCGFFQI 77

Query: 78  INHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYGTSFNPKMEKVFFWRDYL 137
            NHGV E  + +M++  REFF   E E+ ++ +       R  TSFN   EKV  WRD+L
Sbjct: 78  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFL 137

Query: 138 KIMVHP--NF--HAPTKPSRFREISEEYCRRVRELARELARGISESLGLEGCTLEKAVNL 197
           ++  +P  +F    P+ P  FRE++ EY   VR L   L   ISESLGL    +   +  
Sbjct: 138 RLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG- 197

Query: 198 KSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQILHQGKWVNVNPIPN 257
               Q +  N YP CPQ E+  GLP H D  L+T +LQ+++ GLQ+   GKW+ VNP+PN
Sbjct: 198 -KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPN 257

Query: 258 SFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDTLVGPAPELVH-QLT 317
           +F+VN  DQ+++ SN KYKS+LHRA++N    R+S+     PS D ++ PA EL++ +  
Sbjct: 258 TFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEED 317

Query: 318 NPPLYKHIKYKDYMELVQTNDLQHKSMLD 342
           +P +Y++  Y +Y E         +S +D
Sbjct: 318 SPAIYRNFTYAEYFEKFWDTAFDTESCID 341

BLAST of MS012325 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 214.2 bits (544), Expect = 1.7e-55
Identity = 120/304 (39.47%), Postives = 176/304 (57.89%), Query Frame = 0

Query: 46  DFSLLTMGTPDQRAEVVDQLGKACQDWGFFMVINHGVAETVMTEMVDICREFFDLEEEEK 105
           DF L+ + + D R+ ++ Q+ +AC  +GFF VINHGV + ++ EMV + REFF +  EEK
Sbjct: 37  DFPLIDLSSTD-RSFLIQQIHQACARFGFFQVINHGVNKQIIDEMVSVAREFFSMSMEEK 96

Query: 106 REYETKHVLDPIRYGTSFNPKMEKVFFWRDYLKIMVHPNFHA-----PTKPSRFREISEE 165
            +  +       R  TSFN K E+V  WRDYL++  +P  H      P+ P  F+EI  +
Sbjct: 97  MKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLRLHCYP-IHKYVNEWPSNPPSFKEIVSK 156

Query: 166 YCRRVRELARELARGISESLGLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLPPH 225
           Y R VRE+  ++   ISESLGLE   ++K   L    Q +  N YPPCP+ E+  GLP H
Sbjct: 157 YSREVREVGFKIEELISESLGLEKDYMKKV--LGEQGQHMAVNYYPPCPEPELTYGLPAH 216

Query: 226 SDHCLLTFILQN-QICGLQILHQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRAI 285
           +D   LT +LQ+  +CGLQIL  G+W  VNP P++F++N  DQL+  SNG YKS+ HRA+
Sbjct: 217 TDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINIGDQLQALSNGVYKSVWHRAV 276

Query: 286 LNKKATRMSVGIAIGPSLDTLVGPAPEL--VHQLTNPPLYKHIKYKDYMELVQTNDLQHK 342
            N +  R+SV   + P+   ++ PA  L         P+YK   Y +Y +   + +L  +
Sbjct: 277 TNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPVYKDFTYAEYYKKFWSRNLDQE 336

BLAST of MS012325 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 211.5 bits (537), Expect = 1.1e-54
Identity = 117/333 (35.14%), Postives = 188/333 (56.46%), Query Frame = 0

Query: 18  IPSSYVF-SADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQRAEVVDQLGKACQDWGFFM 77
           +P  YV   +   A+ ++    E ++P +D SLL    P  R+  + ++  AC+++GFF 
Sbjct: 27  VPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQ--PFLRSLAIHEISMACKEFGFFQ 86

Query: 78  VINHGVAETVMTEMVDICREFFDLEEEEKREYETKHVLDPIRYGTSFNPKMEKVFFWRDY 137
           VINHG+  +V+ + +D   +FFDL  EEK    + +V +P+RYGTS N   ++V +WRD+
Sbjct: 87  VINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVRYGTSLNHSTDRVHYWRDF 146

Query: 138 LKIMVHPNFH----APTKPSRFREISEEYCRRVRELARELARGISESLGLEGCTLEKAVN 197
           +K   HP        P+ P  +++   +Y      L ++L   ISESLGLE   L++   
Sbjct: 147 IKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQE--E 206

Query: 198 LKSCSQILVGNLYPPCPQAEVAMGLPPHSDHCLLTFILQNQICGLQILHQGK-WVNVNPI 257
           ++  SQ++  N YP CP+ E+A+G+PPHSD   LT +LQ+   GLQI+   K WV V  I
Sbjct: 207 IEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSS-KGLQIMDCNKNWVCVPYI 266

Query: 258 PNSFLVNTADQLEIFSNGKYKSLLHRAILNKKATRMSVGIAIGPSLDTLVGPAPELVHQL 317
             + +V   DQ+E+ SNG YKS++HR  +NK+  R+S        L   + PAP+LV+  
Sbjct: 267 EGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNP- 326

Query: 318 TNPPLYKHIKYKDYMELVQTNDLQHKSMLDLVR 345
            N P Y    + D++  + +ND   +  +D ++
Sbjct: 327 NNAPAYGEFSFNDFLNYISSNDFIQERFIDTIK 353