MS012187 (gene) Bitter gourd (TR) v1

Overview
NameMS012187
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAmino acid permease family protein
Locationscaffold797: 261313 .. 263317 (+)
RNA-Seq ExpressionMS012187
SyntenyMS012187
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGAAGAGAGAGGGGCCGATGCTAAAATTGCTCCAAAACTAACGATTCTTCCTCTCGTCGCTTTGATTTTCTATGACGTTTCTGGGGGACCCTTTGGAGTGGAGGATTCGGTGAGCACCGGCGGCGGCCCGCTTCTGGCGATACTGGGTTTCTTGATTTTTCCCTTTATCTGGAGCATACCGGAGGCGCTGGTCACGGCGGAGCTCGCGACGAGTTTCCCCCAAAACGGCGGGTATGTGGTGTGGATTTCGGCCGCTTTTGGGCCTTTTTGGGGGTTTCAAGAGGGCTTCTGGAAATGGTTCAGTGGCGCAATGGACAATGCTCTGTACCCTGTTTTGTTTCTCGATTACTTGAAGCGTTCTTTCCCTGTTTTGAACCATTTATTCGCTCGAATTCCAGCTCTGTTTGGAATCACTGTCTCTTTAACTTATTTGAACTATCGTGGTCTGCACATTGTTGGGTTTTCTGCTGTTGTTCTTGCTGTGTTTTCGCTTTGCCCTTTTGTGGTGATGGCCATTCTTTCCATCCCCAGAATAAGGCCCAAACGGTGGCTAGTTGTGGAGTTTAGCAAGGTGAATTGGAGGGGTTATTTCAACAGTATGTTTTGGAATCTGAACTATTGGGATAAGGCAAGTACTGTGGCAGGAGAGGTTGAGAATCCAAGCAAAACTTTCCCCAAGGCCATGTTTGGAGCTGTGGTTTTGGTGGTTTCTTCTTATTTAATTCCTCTTCTGGCTGGGACAGGTGCCTTGGAGTCAAATTCAAGTGAGTGGAGTGATGGGTATTTTGCAGAGGTTGGGGCCTTGATTGGTGGGGTGTGGCTCAAATGGTGGATTGAAGCTGCTGCTGCCATGTCTAACATGGGCTTGTTTGAGGCTGAGATGAGCAGTGATGCTTTCCAACTTCTTGGGATGAGTGAGATGGGAATGATCCCTTCTGTGTTTGCTTCAAGGTTGGTGGTTCTGTTCAGTTTCCTTTTTATTCTTCTGATTTCATGGCAACCTTGAGTGACAATAATCTCTCCTTATGTTCCTGCAATTGAGTTTCCTACCTCAAATTGGTAGTAGTGATTCGAAAACTGCCATTTCTAGGGAGAGGTCAGTGGTTCAAATCACCATAAACTTGAAGTAAAACTCGTGAGCTCAGATGGGGCAAGATAACAGTTAAGAGAACAATATGGCAAGATGAATTTCGACAGGATTGTTATAGTAAAAGTACCATAAAAACCCAAAAGCTACAAGAACCCAACGGGCGTATTTAGCTTACGAGCTAAGTTTGTATATACCAAAAGCTTCCTATGACTCCTGAATCATGCCTCTCATGCTACACATTCAAACTCAAGTTTATAACCTGCACGTCTTAAACACAGGCTATACAAACTCAAACTTATTAAAACCGCGAACGAAACCTTCCTGAAGTGCTTATCTCCATATCCAAGTGGGCTGTGAAGCACTTTGAACCATTTTATTTACAAGCTTGTTGTGCTTACACAAAATCTGATCTTATTGAATTACTGTCTCTAATGCACTTTGCAGATCAAAATATGGGACACCCACATTCAGCATTCTGTGCTCTGCTTTTGGGGTTATCTTCCTCTCCTGGATGAGTTTCCAAGAAATTCTTGAGTTTCTCAACTTCCTATACTCCATAGGAATGCTTCTGGAGCTTGCTGCCTTTGTAAAACTAAGAATAAAGAAGCCAGACCTCCACAGACCCTACAGAGTGCCCCTACAAACATTTGGAGCCACAATGCTTTGCCTACCACCTGCTCTCTTGCTTGTTCTTGTGATGAGTTTAGCTTCTTTAAGAACATTCTTAGTCAGTGGTTTTATAATTGCTGTGGGATTCCTTCTATACCCTACTCTTTTGCTGGCAAAGAGCAGAAGATGGGCCAAATTCATTTCACAACATCCTGCAGCTCCTCTTCCTGATGTGGAAGATCAGCCAGCCGAGTCGCAGCCGCTGCCCGATGAGGCTGCAGTTCGGCTTCTTTCAGAATCC

mRNA sequence

ATGGGGGAAGAGAGAGGGGCCGATGCTAAAATTGCTCCAAAACTAACGATTCTTCCTCTCGTCGCTTTGATTTTCTATGACGTTTCTGGGGGACCCTTTGGAGTGGAGGATTCGGTGAGCACCGGCGGCGGCCCGCTTCTGGCGATACTGGGTTTCTTGATTTTTCCCTTTATCTGGAGCATACCGGAGGCGCTGGTCACGGCGGAGCTCGCGACGAGTTTCCCCCAAAACGGCGGGTATGTGGTGTGGATTTCGGCCGCTTTTGGGCCTTTTTGGGGGTTTCAAGAGGGCTTCTGGAAATGGTTCAGTGGCGCAATGGACAATGCTCTGTACCCTGTTTTGTTTCTCGATTACTTGAAGCGTTCTTTCCCTGTTTTGAACCATTTATTCGCTCGAATTCCAGCTCTGTTTGGAATCACTGTCTCTTTAACTTATTTGAACTATCGTGGTCTGCACATTGTTGGGTTTTCTGCTGTTGTTCTTGCTGTGTTTTCGCTTTGCCCTTTTGTGGTGATGGCCATTCTTTCCATCCCCAGAATAAGGCCCAAACGGTGGCTAGTTGTGGAGTTTAGCAAGGTGAATTGGAGGGGTTATTTCAACAGTATGTTTTGGAATCTGAACTATTGGGATAAGGCAAGTACTGTGGCAGGAGAGGTTGAGAATCCAAGCAAAACTTTCCCCAAGGCCATGTTTGGAGCTGTGGTTTTGGTGGTTTCTTCTTATTTAATTCCTCTTCTGGCTGGGACAGGTGCCTTGGAGTCAAATTCAAGTGAGTGGAGTGATGGGTATTTTGCAGAGGTTGGGGCCTTGATTGGTGGGGTGTGGCTCAAATGGTGGATTGAAGCTGCTGCTGCCATGTCTAACATGGGCTTGTTTGAGGCTGAGATGAGCAGTGATGCTTTCCAACTTCTTGGGATGAGTGAGATGGGAATGATCCCTTCTGTGTTTGCTTCAAGATCAAAATATGGGACACCCACATTCAGCATTCTGTGCTCTGCTTTTGGGGTTATCTTCCTCTCCTGGATGAGTTTCCAAGAAATTCTTGAGTTTCTCAACTTCCTATACTCCATAGGAATGCTTCTGGAGCTTGCTGCCTTTGTAAAACTAAGAATAAAGAAGCCAGACCTCCACAGACCCTACAGAGTGCCCCTACAAACATTTGGAGCCACAATGCTTTGCCTACCACCTGCTCTCTTGCTTGTTCTTGTGATGAGTTTAGCTTCTTTAAGAACATTCTTAGTCAGTGGTTTTATAATTGCTGTGGGATTCCTTCTATACCCTACTCTTTTGCTGGCAAAGAGCAGAAGATGGGCCAAATTCATTTCACAACATCCTGCAGCTCCTCTTCCTGATGTGGAAGATCAGCCAGCCGAGTCGCAGCCGCTGCCCGATGAGGCTGCAGTTCGGCTTCTTTCAGAATCC

Coding sequence (CDS)

ATGGGGGAAGAGAGAGGGGCCGATGCTAAAATTGCTCCAAAACTAACGATTCTTCCTCTCGTCGCTTTGATTTTCTATGACGTTTCTGGGGGACCCTTTGGAGTGGAGGATTCGGTGAGCACCGGCGGCGGCCCGCTTCTGGCGATACTGGGTTTCTTGATTTTTCCCTTTATCTGGAGCATACCGGAGGCGCTGGTCACGGCGGAGCTCGCGACGAGTTTCCCCCAAAACGGCGGGTATGTGGTGTGGATTTCGGCCGCTTTTGGGCCTTTTTGGGGGTTTCAAGAGGGCTTCTGGAAATGGTTCAGTGGCGCAATGGACAATGCTCTGTACCCTGTTTTGTTTCTCGATTACTTGAAGCGTTCTTTCCCTGTTTTGAACCATTTATTCGCTCGAATTCCAGCTCTGTTTGGAATCACTGTCTCTTTAACTTATTTGAACTATCGTGGTCTGCACATTGTTGGGTTTTCTGCTGTTGTTCTTGCTGTGTTTTCGCTTTGCCCTTTTGTGGTGATGGCCATTCTTTCCATCCCCAGAATAAGGCCCAAACGGTGGCTAGTTGTGGAGTTTAGCAAGGTGAATTGGAGGGGTTATTTCAACAGTATGTTTTGGAATCTGAACTATTGGGATAAGGCAAGTACTGTGGCAGGAGAGGTTGAGAATCCAAGCAAAACTTTCCCCAAGGCCATGTTTGGAGCTGTGGTTTTGGTGGTTTCTTCTTATTTAATTCCTCTTCTGGCTGGGACAGGTGCCTTGGAGTCAAATTCAAGTGAGTGGAGTGATGGGTATTTTGCAGAGGTTGGGGCCTTGATTGGTGGGGTGTGGCTCAAATGGTGGATTGAAGCTGCTGCTGCCATGTCTAACATGGGCTTGTTTGAGGCTGAGATGAGCAGTGATGCTTTCCAACTTCTTGGGATGAGTGAGATGGGAATGATCCCTTCTGTGTTTGCTTCAAGATCAAAATATGGGACACCCACATTCAGCATTCTGTGCTCTGCTTTTGGGGTTATCTTCCTCTCCTGGATGAGTTTCCAAGAAATTCTTGAGTTTCTCAACTTCCTATACTCCATAGGAATGCTTCTGGAGCTTGCTGCCTTTGTAAAACTAAGAATAAAGAAGCCAGACCTCCACAGACCCTACAGAGTGCCCCTACAAACATTTGGAGCCACAATGCTTTGCCTACCACCTGCTCTCTTGCTTGTTCTTGTGATGAGTTTAGCTTCTTTAAGAACATTCTTAGTCAGTGGTTTTATAATTGCTGTGGGATTCCTTCTATACCCTACTCTTTTGCTGGCAAAGAGCAGAAGATGGGCCAAATTCATTTCACAACATCCTGCAGCTCCTCTTCCTGATGTGGAAGATCAGCCAGCCGAGTCGCAGCCGCTGCCCGATGAGGCTGCAGTTCGGCTTCTTTCAGAATCC

Protein sequence

MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWSIPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRIRPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDAFQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIAVGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQPLPDEAAVRLLSES
Homology
BLAST of MS012187 vs. NCBI nr
Match: XP_022148759.1 (probable polyamine transporter At3g19553 [Momordica charantia])

HSP 1 Score: 927.9 bits (2397), Expect = 3.4e-266
Identity = 473/474 (99.79%), Postives = 473/474 (99.79%), Query Frame = 0

Query: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60
           MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS
Sbjct: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFG FWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGHFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180
           RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI
Sbjct: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180

Query: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240
           RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300

Query: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360
           FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML
Sbjct: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420
           LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA
Sbjct: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420

Query: 421 VGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQPLPDEAAVRLLSES 475
           VGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQPLPDEAAVRLLSES
Sbjct: 421 VGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQPLPDEAAVRLLSES 474

BLAST of MS012187 vs. NCBI nr
Match: XP_038888395.1 (probable polyamine transporter At3g19553 [Benincasa hispida])

HSP 1 Score: 839.0 bits (2166), Expect = 2.1e-239
Identity = 420/477 (88.05%), Postives = 446/477 (93.50%), Query Frame = 0

Query: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60
           MG+ER ADAKIA KLTILPL+ALIFYDVSGGPFGVEDSVSTGGGPLLA+LGFL+FPFIWS
Sbjct: 1   MGDERRADAKIAQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180
           RSFPV NH+FARIP L GIT SLTYLNYRGLHIVG SAVVLA FSLCPFVVM +LSIPRI
Sbjct: 121 RSFPVFNHVFARIPTLLGITASLTYLNYRGLHIVGVSAVVLAAFSLCPFVVMTLLSIPRI 180

Query: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240
           RPK+WLVVE+SKVNWRGYFNSMFWNLNYWDKAST+AGEVENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALES+SSEWSDGYFAEVGALIGGVWLKWWI+AAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360
           +QLLGMSEMGM+PSVFA+RSKYGTPTFSILCSA GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMLPSVFAARSKYGTPTFSILCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420
           LE AAF+KLRIKKPDL+RPYRVPLQTFG TMLC PPA LLVLVM LAS +TFL+SG I+ 
Sbjct: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVT 420

Query: 421 VGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAES---QPLPDEAAVRLLSES 475
           VGFLLYPTLL AK++RW KFIS+ P A LPDVED+  ES   Q +P+EA VRLLSES
Sbjct: 421 VGFLLYPTLLQAKNKRWVKFISEQPEATLPDVEDRLVESHAQQEVPNEAEVRLLSES 477

BLAST of MS012187 vs. NCBI nr
Match: XP_004136943.1 (probable polyamine transporter At3g19553 [Cucumis sativus] >KGN43858.1 hypothetical protein Csa_017296 [Cucumis sativus])

HSP 1 Score: 824.3 bits (2128), Expect = 5.3e-235
Identity = 415/478 (86.82%), Postives = 443/478 (92.68%), Query Frame = 0

Query: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60
           MG+ER  DAKI  KLTILPL+ALIFYDVSGGPFGVEDSVSTGGGPLLA+LGFL+FPFIWS
Sbjct: 1   MGDERRVDAKITQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180
           RSFPV NH+FARIPAL GIT SLTYLNYRGLHIVG SAVVLAVFSLCPFVVM +LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240
            PK+WLVVE+SKVNWRGYFNSMFWNLNYWDKAST+AGEVENPSKTFPKAMFGAVVLVVS 
Sbjct: 181 SPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSF 240

Query: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALE++SSEWSDGYFAEVGALIGGVWLKWWI+AAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALETDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360
           +QLLGMSEMGM+PSVFASRSKYGTPTFSILCSA GVIFLSWMSFQEILEFLNFLY+IGML
Sbjct: 301 YQLLGMSEMGMLPSVFASRSKYGTPTFSILCSALGVIFLSWMSFQEILEFLNFLYAIGML 360

Query: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420
           LE AAF+KLRI+KPDL+RPY+VPLQTFG T+LC PPA LL LVM LAS +TFL+SG IIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTFGVTLLCFPPAALLFLVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLLAKSRRWAKFISQHPA-APLPDVEDQPAES---QPLPDEAAVRLLSES 475
           VGFLLYPTLL AK+RRW KFIS+ P    LPDVED+  ES   Q +P+EA VRLLSES
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLPDVEDRLVESQQQQEVPNEAEVRLLSES 478

BLAST of MS012187 vs. NCBI nr
Match: XP_023553804.1 (probable polyamine transporter At3g19553 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 822.8 bits (2124), Expect = 1.5e-234
Identity = 416/477 (87.21%), Postives = 442/477 (92.66%), Query Frame = 0

Query: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60
           MG+ER   A IAPKLTILPL+ALIFYDVSGGPFGVEDSVSTGGGPLLA+LGFL+FPFIWS
Sbjct: 1   MGDER---ANIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180
           RSFPV +H+FARIPAL GIT SLTYLNYRGLHIVGFSAVVLAVFSLCPFVVM ILSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240
           RP++WLVV++S VNWRGYFNSMFWNLNYWDKAST+AGEVENPSKTFPKAMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALE++SSEWSDGYFAEVGALIGG WLKWWI+AAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360
           +QLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIFLSWMSFQEILEFLNFLYSIGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420
           LELAAF+KLR+KKPDLHRPY+VPLQTFGA MLCLPPA LLVLVM LAS +TFL+SG IIA
Sbjct: 361 LELAAFIKLRMKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIA 420

Query: 421 VGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQP---LPDEAAVRLLSES 475
           VGFLLYP+L  AK+R W KFIS    A LPDVED+  E QP   + DEA +RLLSES
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISDEQGATLPDVEDRLVEPQPREEVLDEAVIRLLSES 474

BLAST of MS012187 vs. NCBI nr
Match: KAA0031359.1 (putative polyamine transporter [Cucumis melo var. makuwa] >TYK06811.1 putative polyamine transporter [Cucumis melo var. makuwa])

HSP 1 Score: 821.2 bits (2120), Expect = 4.5e-234
Identity = 412/478 (86.19%), Postives = 443/478 (92.68%), Query Frame = 0

Query: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60
           MG+ER  DAKIA KLTILPL+ALIFYDVSGGPFGVEDSVSTGGGPLLA+LGFL+FPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180
           RSFPV NH+FARIPAL GIT SLTYLNYRGLHIVG SAVVLAVFSLCPFVVM +LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240
            PK+WL++E+SKVNWRGYFNSMFWNLNYWDKAST+AGEVENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL ++SSEWSDGYFAEVGALIGGVWLKWWI+AAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360
           +QLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420
           LE AAF+KLRI+KPDL+RPY+VPLQT G T+LC PP+ LL+LVM LAS +TFL+SG IIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLLAKSRRWAKFISQHPA-APLPDVEDQPAESQP---LPDEAAVRLLSES 475
           VGFLLYPTLL AK+RRW KFIS+ P    L DVED+  E QP   +P+EA VRLLSES
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSES 478

BLAST of MS012187 vs. ExPASy Swiss-Prot
Match: Q9LH39 (Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g19553 PE=3 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 6.8e-209
Identity = 361/472 (76.48%), Postives = 412/472 (87.29%), Query Frame = 0

Query: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60
           + +E  +  K +PKLT+LPLV LIFY+VSGGPFGVEDSV +GGGPLLA+LGFLIFP IWS
Sbjct: 8   VNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWS 67

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATSFP+NGGYVVWIS+AFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 68  IPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 127

Query: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180
            SFPVL+H+ AR+PAL  IT SLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMA+L++P I
Sbjct: 128 HSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNI 187

Query: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240
           RPKRWL V+  K+NWRGYFN+MFWNLNYWDKAST+AGEV+ P KTFPKA+FGAV+LV+ S
Sbjct: 188 RPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGS 247

Query: 241 YLIPLLAGTGALESNSS-EWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSD 300
           YLIPL+AGTGAL S++S EWSDGYFAEVG LIGGVWLK WI+AAAAMSN+GLFEAEMSSD
Sbjct: 248 YLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSD 307

Query: 301 AFQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGM 360
           AFQLLGMSE+GM+P+ FA RSKYGTPT SILCSA GVIFLSWMSFQEI+EFLNFLY++GM
Sbjct: 308 AFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGM 367

Query: 361 LLELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFII 420
           LLE AAFVKLRIKKPDLHRPYRVPL TFG +MLCLPP+LL++LVM LA+ +TFL+SG II
Sbjct: 368 LLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVII 427

Query: 421 AVGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQPLPDEAAVRLL 472
            +GF LYP L L K ++WA+FI +    P+  V  +    +   DE+A  LL
Sbjct: 428 VLGFCLYPFLTLVKEKQWARFIPEE-TRPVSGVSSESQLDEEHGDESAASLL 478

BLAST of MS012187 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 3.2e-150
Identity = 255/445 (57.30%), Postives = 338/445 (75.96%), Query Frame = 0

Query: 14  KLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWSIPEALVTAELATS 73
           K+++LPLV LIFY+VSGGPFGVEDSV+   GPLLA+LGF+IFPFIWSIPEAL+TAE+ T 
Sbjct: 48  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 107

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVLNHLFARI 133
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW SG +DNALYPVLFLDYLK   P L     R+
Sbjct: 108 YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 167

Query: 134 PALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRIRPKRWLVVEFSKV 193
            ++  +T+ LTYLNYRGL IVG+ AV++ VFS+ PF VM ++SIP++ P RWLV++   V
Sbjct: 168 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 227

Query: 194 NWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++FWNLNYWD  ST+AGEVENP+ T PKA+F  V+LV  SY+ PLLAG GA+ 
Sbjct: 228 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 287

Query: 254 SNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDAFQLLGMSEMGMIP 313
               +W+DGYF++V   +GG WL+WW++AAAA SNMG+F AEMSSD+FQLLGM+E GM+P
Sbjct: 288 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 347

Query: 314 SVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFVKLRIKK 373
             FA RS+YGTP   IL SA GV+ LSW+SFQEI+   N LY +GM+LE  AFV++R+K 
Sbjct: 348 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 407

Query: 374 PDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIAVGFLLYPTLLLAK 433
           P   RPY++P+ T G+ ++C+PP +L+  V++L+SL+   VS  ++ +GFL++P L    
Sbjct: 408 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 467

Query: 434 SRRWAKFISQHPAAPLPDVEDQPAE 459
            +RW KF     ++ LPD++ Q  E
Sbjct: 468 RKRWVKF---SISSDLPDLQQQTRE 488

BLAST of MS012187 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 3.9e-148
Identity = 253/429 (58.97%), Postives = 329/429 (76.69%), Query Frame = 0

Query: 14  KLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWSIPEALVTAELATS 73
           K+T+LPLV LIFY+VSGGPFG+EDSV    GPLLAI+GF++FPFIWSIPEAL+TAE+ T 
Sbjct: 52  KITVLPLVFLIFYEVSGGPFGIEDSVK-AAGPLLAIVGFIVFPFIWSIPEALITAEMGTM 111

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVLNHLFARI 133
           FP+NGGYVVW++ A GP+WGFQ+G+ KW SG +DNALYP+LFLDYLK   P+L     R+
Sbjct: 112 FPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRV 171

Query: 134 PALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRIRPKRWLVV--EFS 193
            A+  +TV+LTYLNYRGL IVG +AV+L VFS+ PFVVM+ +SIP+++P RWLVV  +  
Sbjct: 172 AAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMK 231

Query: 194 KVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGA 253
            VNW  Y N++FWNLNYWD  ST+ GEVENPSKT P+A+F A++LVV SY+ P+L GTGA
Sbjct: 232 GVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGA 291

Query: 254 LESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDAFQLLGMSEMGM 313
           +  +   W+DGYFA++G +IGGVWL WWI+AAAA SNMG+F AEMSSD+FQLLGM+E GM
Sbjct: 292 IALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGM 351

Query: 314 IPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFVKLRI 373
           +P VFA RS+Y TP   IL SA GVI LSW+SFQEI+   N LY  GM+LE   FV+LR+
Sbjct: 352 LPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRM 411

Query: 374 KKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIAVGFLLYPTLLL 433
           K P   RP+++P+   G+ ++C+PP +L+ ++M+  +L+  LVS   I +G +L P L  
Sbjct: 412 KYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQ 471

Query: 434 AKSRRWAKF 441
            + + W KF
Sbjct: 472 VEKKGWLKF 479

BLAST of MS012187 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 2.4e-145
Identity = 258/467 (55.25%), Postives = 339/467 (72.59%), Query Frame = 0

Query: 2   GEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWSI 61
           G+  G     A  ++++PL+ LIFY+VSGGPFG+EDSV    GPLLAI+GFL+ P IWSI
Sbjct: 70  GDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVG-AAGPLLAIIGFLVLPVIWSI 129

Query: 62  PEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKR 121
           PEAL+TAEL   FP+NGGYVVW+++A GP+WGFQ+G+ KW SG +DNALYPVLFLDYLK 
Sbjct: 130 PEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKS 189

Query: 122 SFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRIR 181
             P L     R  A+ G+T  LT LNYRGL +VG+ A+ L VFSL PF VM ++++P++R
Sbjct: 190 GVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLR 249

Query: 182 PKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSSY 241
           P RWLV++   V+W  Y N++FWNLNYWD  ST+AGEV+NP KT PKA+F AV+ VV +Y
Sbjct: 250 PARWLVIDLHNVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAY 309

Query: 242 LIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDAF 301
           L PLLAGTGA+  +  +W+DGYFA++  L+GG WL WW+++AAA+SNMG+F AEMSSD++
Sbjct: 310 LYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSY 369

Query: 302 QLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGMLL 361
           QLLGM+E GM+PS FA+RS+YGTP   IL SA GV+ LS MSFQEI+   NFLY  GMLL
Sbjct: 370 QLLGMAERGMLPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLL 429

Query: 362 ELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIAV 421
           E  AF+  R+++PD  RPYRVPL T G   + +PP  L+ +V++L++L+  +VS   +A+
Sbjct: 430 EFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAM 489

Query: 422 GFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQPLPDEAAV 469
           G +L P L   + +RW +F S +P  P   V   PA     PDE  V
Sbjct: 490 GLVLQPALRFVEKKRWLRF-SVNPDLPEIGVIRPPA----APDEPLV 530

BLAST of MS012187 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 2.4e-145
Identity = 258/467 (55.25%), Postives = 339/467 (72.59%), Query Frame = 0

Query: 2   GEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWSI 61
           G+  G     A  ++++PL+ LIFY+VSGGPFG+EDSV    GPLLAI+GFL+ P IWSI
Sbjct: 70  GDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVG-AAGPLLAIIGFLVLPVIWSI 129

Query: 62  PEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKR 121
           PEAL+TAEL   FP+NGGYVVW+++A GP+WGFQ+G+ KW SG +DNALYPVLFLDYLK 
Sbjct: 130 PEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKS 189

Query: 122 SFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRIR 181
             P L     R  A+ G+T  LT LNYRGL +VG+ A+ L VFSL PF VM ++++P++R
Sbjct: 190 GVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLR 249

Query: 182 PKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSSY 241
           P RWLV++   V+W  Y N++FWNLNYWD  ST+AGEV+NP KT PKA+F AV+ VV +Y
Sbjct: 250 PARWLVIDLHNVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAY 309

Query: 242 LIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDAF 301
           L PLLAGTGA+  +  +W+DGYFA++  L+GG WL WW+++AAA+SNMG+F AEMSSD++
Sbjct: 310 LYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSY 369

Query: 302 QLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGMLL 361
           QLLGM+E GM+PS FA+RS+YGTP   IL SA GV+ LS MSFQEI+   NFLY  GMLL
Sbjct: 370 QLLGMAERGMLPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLL 429

Query: 362 ELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIAV 421
           E  AF+  R+++PD  RPYRVPL T G   + +PP  L+ +V++L++L+  +VS   +A+
Sbjct: 430 EFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAM 489

Query: 422 GFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQPLPDEAAV 469
           G +L P L   + +RW +F S +P  P   V   PA     PDE  V
Sbjct: 490 GLVLQPALRFVEKKRWLRF-SVNPDLPEIGVIRPPA----APDEPLV 530

BLAST of MS012187 vs. ExPASy TrEMBL
Match: A0A6J1D4Y1 (probable polyamine transporter At3g19553 OS=Momordica charantia OX=3673 GN=LOC111017345 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 1.6e-266
Identity = 473/474 (99.79%), Postives = 473/474 (99.79%), Query Frame = 0

Query: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60
           MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS
Sbjct: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFG FWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGHFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180
           RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI
Sbjct: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180

Query: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240
           RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300

Query: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360
           FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML
Sbjct: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420
           LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA
Sbjct: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420

Query: 421 VGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQPLPDEAAVRLLSES 475
           VGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQPLPDEAAVRLLSES
Sbjct: 421 VGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQPLPDEAAVRLLSES 474

BLAST of MS012187 vs. ExPASy TrEMBL
Match: A0A0A0K2Y2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071470 PE=3 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 2.6e-235
Identity = 415/478 (86.82%), Postives = 443/478 (92.68%), Query Frame = 0

Query: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60
           MG+ER  DAKI  KLTILPL+ALIFYDVSGGPFGVEDSVSTGGGPLLA+LGFL+FPFIWS
Sbjct: 1   MGDERRVDAKITQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180
           RSFPV NH+FARIPAL GIT SLTYLNYRGLHIVG SAVVLAVFSLCPFVVM +LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240
            PK+WLVVE+SKVNWRGYFNSMFWNLNYWDKAST+AGEVENPSKTFPKAMFGAVVLVVS 
Sbjct: 181 SPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSF 240

Query: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALE++SSEWSDGYFAEVGALIGGVWLKWWI+AAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALETDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360
           +QLLGMSEMGM+PSVFASRSKYGTPTFSILCSA GVIFLSWMSFQEILEFLNFLY+IGML
Sbjct: 301 YQLLGMSEMGMLPSVFASRSKYGTPTFSILCSALGVIFLSWMSFQEILEFLNFLYAIGML 360

Query: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420
           LE AAF+KLRI+KPDL+RPY+VPLQTFG T+LC PPA LL LVM LAS +TFL+SG IIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTFGVTLLCFPPAALLFLVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLLAKSRRWAKFISQHPA-APLPDVEDQPAES---QPLPDEAAVRLLSES 475
           VGFLLYPTLL AK+RRW KFIS+ P    LPDVED+  ES   Q +P+EA VRLLSES
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLPDVEDRLVESQQQQEVPNEAEVRLLSES 478

BLAST of MS012187 vs. ExPASy TrEMBL
Match: A0A5D3C4H2 (Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001140 PE=3 SV=1)

HSP 1 Score: 821.2 bits (2120), Expect = 2.2e-234
Identity = 412/478 (86.19%), Postives = 443/478 (92.68%), Query Frame = 0

Query: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60
           MG+ER  DAKIA KLTILPL+ALIFYDVSGGPFGVEDSVSTGGGPLLA+LGFL+FPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180
           RSFPV NH+FARIPAL GIT SLTYLNYRGLHIVG SAVVLAVFSLCPFVVM +LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240
            PK+WL++E+SKVNWRGYFNSMFWNLNYWDKAST+AGEVENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL ++SSEWSDGYFAEVGALIGGVWLKWWI+AAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360
           +QLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420
           LE AAF+KLRI+KPDL+RPY+VPLQT G T+LC PP+ LL+LVM LAS +TFL+SG IIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLLAKSRRWAKFISQHPA-APLPDVEDQPAESQP---LPDEAAVRLLSES 475
           VGFLLYPTLL AK+RRW KFIS+ P    L DVED+  E QP   +P+EA VRLLSES
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSES 478

BLAST of MS012187 vs. ExPASy TrEMBL
Match: A0A6J1I9Z5 (probable polyamine transporter At3g19553 OS=Cucurbita maxima OX=3661 GN=LOC111471008 PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 2.8e-234
Identity = 414/477 (86.79%), Postives = 442/477 (92.66%), Query Frame = 0

Query: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60
           MG+ER   AKIAPKLTILPL+ALIFYDVSGGPFGVEDSVSTGGGPLLA+LGFL+FPFIWS
Sbjct: 1   MGDER---AKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180
           RSFPV +H+FARIPAL GIT SLTYLNYRGLHIVGFSAVVLAVFSLCPFVVM ILSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240
           RP++WLVV++S VNWRGYFNSMFWNLNYWDKAST+AGEVENPSKTFPKAMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALE++SS+WSDGYFAEVGALIGG WLKWWI+AAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSDWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360
           +QLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIFLSWMSFQEILEFLNFLYSIGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420
           LELAAF+KLRIKKPDLHRPY+VPLQTFGA MLCLPPA LLVLVM LAS +TFL+SG II+
Sbjct: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGIIIS 420

Query: 421 VGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVED---QPAESQPLPDEAAVRLLSES 475
           VGFLLYP+L  AK+R W KFIS+     LPDVED   +P   Q + DEA +RLLSES
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDEAVIRLLSES 474

BLAST of MS012187 vs. ExPASy TrEMBL
Match: A0A1S3C014 (probable polyamine transporter At3g19553 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495296 PE=3 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 4.8e-234
Identity = 411/478 (85.98%), Postives = 443/478 (92.68%), Query Frame = 0

Query: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60
           MG+ER  DAKIA KLTILPL+ALIFYDVSGGPFGVEDSVSTGGGPLLA+LGFL+FPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180
           RSFPV NH+FARIPAL GIT SLTYLNYRGLHIVG SAVVLAVFSLCPFVVM +LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240
            PK+WL++E+SKVNWRGYFNSMFWNLNYWDKAST+AGEVENPSKTFP+AMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL ++SSEWSDGYFAEVGALIGGVWLKWWI+AAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360
           +QLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420
           LE AAF+KLRI+KPDL+RPY+VPLQT G T+LC PP+ LL+LVM LAS +TFL+SG IIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLLAKSRRWAKFISQHPA-APLPDVEDQPAESQP---LPDEAAVRLLSES 475
           VGFLLYPTLL AK+RRW KFIS+ P    L DVED+  E QP   +P+EA VRLLSES
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSES 478

BLAST of MS012187 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 728.0 bits (1878), Expect = 4.8e-210
Identity = 361/472 (76.48%), Postives = 412/472 (87.29%), Query Frame = 0

Query: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60
           + +E  +  K +PKLT+LPLV LIFY+VSGGPFGVEDSV +GGGPLLA+LGFLIFP IWS
Sbjct: 8   VNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWS 67

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATSFP+NGGYVVWIS+AFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 68  IPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 127

Query: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180
            SFPVL+H+ AR+PAL  IT SLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMA+L++P I
Sbjct: 128 HSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNI 187

Query: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240
           RPKRWL V+  K+NWRGYFN+MFWNLNYWDKAST+AGEV+ P KTFPKA+FGAV+LV+ S
Sbjct: 188 RPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGS 247

Query: 241 YLIPLLAGTGALESNSS-EWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSD 300
           YLIPL+AGTGAL S++S EWSDGYFAEVG LIGGVWLK WI+AAAAMSN+GLFEAEMSSD
Sbjct: 248 YLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSD 307

Query: 301 AFQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGM 360
           AFQLLGMSE+GM+P+ FA RSKYGTPT SILCSA GVIFLSWMSFQEI+EFLNFLY++GM
Sbjct: 308 AFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGM 367

Query: 361 LLELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFII 420
           LLE AAFVKLRIKKPDLHRPYRVPL TFG +MLCLPP+LL++LVM LA+ +TFL+SG II
Sbjct: 368 LLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVII 427

Query: 421 AVGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQPLPDEAAVRLL 472
            +GF LYP L L K ++WA+FI +    P+  V  +    +   DE+A  LL
Sbjct: 428 VLGFCLYPFLTLVKEKQWARFIPEE-TRPVSGVSSESQLDEEHGDESAASLL 478

BLAST of MS012187 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 533.1 bits (1372), Expect = 2.3e-151
Identity = 255/445 (57.30%), Postives = 338/445 (75.96%), Query Frame = 0

Query: 14  KLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWSIPEALVTAELATS 73
           K+++LPLV LIFY+VSGGPFGVEDSV+   GPLLA+LGF+IFPFIWSIPEAL+TAE+ T 
Sbjct: 48  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 107

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVLNHLFARI 133
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW SG +DNALYPVLFLDYLK   P L     R+
Sbjct: 108 YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 167

Query: 134 PALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRIRPKRWLVVEFSKV 193
            ++  +T+ LTYLNYRGL IVG+ AV++ VFS+ PF VM ++SIP++ P RWLV++   V
Sbjct: 168 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 227

Query: 194 NWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++FWNLNYWD  ST+AGEVENP+ T PKA+F  V+LV  SY+ PLLAG GA+ 
Sbjct: 228 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 287

Query: 254 SNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDAFQLLGMSEMGMIP 313
               +W+DGYF++V   +GG WL+WW++AAAA SNMG+F AEMSSD+FQLLGM+E GM+P
Sbjct: 288 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 347

Query: 314 SVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFVKLRIKK 373
             FA RS+YGTP   IL SA GV+ LSW+SFQEI+   N LY +GM+LE  AFV++R+K 
Sbjct: 348 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 407

Query: 374 PDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIAVGFLLYPTLLLAK 433
           P   RPY++P+ T G+ ++C+PP +L+  V++L+SL+   VS  ++ +GFL++P L    
Sbjct: 408 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 467

Query: 434 SRRWAKFISQHPAAPLPDVEDQPAE 459
            +RW KF     ++ LPD++ Q  E
Sbjct: 468 RKRWVKF---SISSDLPDLQQQTRE 488

BLAST of MS012187 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 533.1 bits (1372), Expect = 2.3e-151
Identity = 255/445 (57.30%), Postives = 338/445 (75.96%), Query Frame = 0

Query: 14  KLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWSIPEALVTAELATS 73
           K+++LPLV LIFY+VSGGPFGVEDSV+   GPLLA+LGF+IFPFIWSIPEAL+TAE+ T 
Sbjct: 32  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 91

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVLNHLFARI 133
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW SG +DNALYPVLFLDYLK   P L     R+
Sbjct: 92  YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 151

Query: 134 PALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRIRPKRWLVVEFSKV 193
            ++  +T+ LTYLNYRGL IVG+ AV++ VFS+ PF VM ++SIP++ P RWLV++   V
Sbjct: 152 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 211

Query: 194 NWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++FWNLNYWD  ST+AGEVENP+ T PKA+F  V+LV  SY+ PLLAG GA+ 
Sbjct: 212 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 271

Query: 254 SNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDAFQLLGMSEMGMIP 313
               +W+DGYF++V   +GG WL+WW++AAAA SNMG+F AEMSSD+FQLLGM+E GM+P
Sbjct: 272 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 331

Query: 314 SVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFVKLRIKK 373
             FA RS+YGTP   IL SA GV+ LSW+SFQEI+   N LY +GM+LE  AFV++R+K 
Sbjct: 332 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 391

Query: 374 PDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIAVGFLLYPTLLLAK 433
           P   RPY++P+ T G+ ++C+PP +L+  V++L+SL+   VS  ++ +GFL++P L    
Sbjct: 392 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 451

Query: 434 SRRWAKFISQHPAAPLPDVEDQPAE 459
            +RW KF     ++ LPD++ Q  E
Sbjct: 452 RKRWVKF---SISSDLPDLQQQTRE 472

BLAST of MS012187 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 526.2 bits (1354), Expect = 2.8e-149
Identity = 253/429 (58.97%), Postives = 329/429 (76.69%), Query Frame = 0

Query: 14  KLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWSIPEALVTAELATS 73
           K+T+LPLV LIFY+VSGGPFG+EDSV    GPLLAI+GF++FPFIWSIPEAL+TAE+ T 
Sbjct: 52  KITVLPLVFLIFYEVSGGPFGIEDSVK-AAGPLLAIVGFIVFPFIWSIPEALITAEMGTM 111

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVLNHLFARI 133
           FP+NGGYVVW++ A GP+WGFQ+G+ KW SG +DNALYP+LFLDYLK   P+L     R+
Sbjct: 112 FPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRV 171

Query: 134 PALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRIRPKRWLVV--EFS 193
            A+  +TV+LTYLNYRGL IVG +AV+L VFS+ PFVVM+ +SIP+++P RWLVV  +  
Sbjct: 172 AAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMK 231

Query: 194 KVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGA 253
            VNW  Y N++FWNLNYWD  ST+ GEVENPSKT P+A+F A++LVV SY+ P+L GTGA
Sbjct: 232 GVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGA 291

Query: 254 LESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDAFQLLGMSEMGM 313
           +  +   W+DGYFA++G +IGGVWL WWI+AAAA SNMG+F AEMSSD+FQLLGM+E GM
Sbjct: 292 IALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGM 351

Query: 314 IPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFVKLRI 373
           +P VFA RS+Y TP   IL SA GVI LSW+SFQEI+   N LY  GM+LE   FV+LR+
Sbjct: 352 LPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRM 411

Query: 374 KKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIAVGFLLYPTLLL 433
           K P   RP+++P+   G+ ++C+PP +L+ ++M+  +L+  LVS   I +G +L P L  
Sbjct: 412 KYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQ 471

Query: 434 AKSRRWAKF 441
            + + W KF
Sbjct: 472 VEKKGWLKF 479

BLAST of MS012187 vs. TAIR 10
Match: AT1G31820.1 (Amino acid permease family protein )

HSP 1 Score: 489.6 bits (1259), Expect = 2.9e-138
Identity = 240/427 (56.21%), Postives = 318/427 (74.47%), Query Frame = 0

Query: 14  KLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWSIPEALVTAELATS 73
           K+++LPLV LIFY+VSGGPFG E SV+   GPLLA+LGF+IFPFIW IPEAL+TAE++T 
Sbjct: 35  KVSMLPLVFLIFYEVSGGPFGAEGSVN-AAGPLLALLGFVIFPFIWCIPEALITAEMSTM 94

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVLNHLFARI 133
           FP NGG+VVW+S+A G FWGFQ G+ KW  G +DNALYPVLFLDYLK + P L     R+
Sbjct: 95  FPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNALYPVLFLDYLKSAVPALATGLPRV 154

Query: 134 PALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRIRPKRWLVVEFSKV 193
            ++  +T+ LTYLNYRGL IVG++AV + VFS+ PF VM+++SIP++ P RWLV++   V
Sbjct: 155 ASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPFAVMSLVSIPQLEPSRWLVMDLGNV 214

Query: 194 NWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++ WNLNYWD  ST+AGEV NP KT PKA+   V+ V  S  +PLL+GTGA+ 
Sbjct: 215 NWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKALCYGVIFVALSNFLPLLSGTGAIP 274

Query: 254 SNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDAFQLLGMSEMGMIP 313
            +   W+DGY AEV   IGG WL+ W++AAAA SNMG+F AEMSSD+FQLLGM+E+G++P
Sbjct: 275 LDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNMGMFLAEMSSDSFQLLGMAELGILP 334

Query: 314 SVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFVKLRIKK 373
            +FA RS+YGTP   IL SA GV+ LS +SFQEI+   N LY  GM+LE  AFV+LR K 
Sbjct: 335 EIFAQRSRYGTPLLGILFSASGVLLLSGLSFQEIIAAENLLYCGGMILEFIAFVRLRKKH 394

Query: 374 PDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIAVGFLLYPTLLLAK 433
           P   RPY++P+ T G+ ++C+PP +L+ LV+ L++++  LVS  ++ +GFL+ P L    
Sbjct: 395 PAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTIKVALVSFVMVVIGFLMKPCLNHMD 454

Query: 434 SRRWAKF 441
            ++W KF
Sbjct: 455 GKKWVKF 460

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148759.13.4e-26699.79probable polyamine transporter At3g19553 [Momordica charantia][more]
XP_038888395.12.1e-23988.05probable polyamine transporter At3g19553 [Benincasa hispida][more]
XP_004136943.15.3e-23586.82probable polyamine transporter At3g19553 [Cucumis sativus] >KGN43858.1 hypotheti... [more]
XP_023553804.11.5e-23487.21probable polyamine transporter At3g19553 [Cucurbita pepo subsp. pepo][more]
KAA0031359.14.5e-23486.19putative polyamine transporter [Cucumis melo var. makuwa] >TYK06811.1 putative p... [more]
Match NameE-valueIdentityDescription
Q9LH396.8e-20976.48Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q9C6S53.2e-15057.30Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
Q9FFL13.9e-14858.97Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
A2X8M82.4e-14555.25Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Q6Z8D02.4e-14555.25Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1D4Y11.6e-26699.79probable polyamine transporter At3g19553 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A0A0K2Y22.6e-23586.82Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071470 PE=3 SV=1[more]
A0A5D3C4H22.2e-23486.19Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A6J1I9Z52.8e-23486.79probable polyamine transporter At3g19553 OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A1S3C0144.8e-23485.98probable polyamine transporter At3g19553 isoform X1 OS=Cucumis melo OX=3656 GN=L... [more]
Match NameE-valueIdentityDescription
AT3G19553.14.8e-21076.48Amino acid permease family protein [more]
AT1G31830.12.3e-15157.30Amino acid permease family protein [more]
AT1G31830.22.3e-15157.30Amino acid permease family protein [more]
AT5G05630.12.8e-14958.97Amino acid permease family protein [more]
AT1G31820.12.9e-13856.21Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 3..462
e-value: 3.1E-66
score: 221.3
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 14..440
e-value: 1.5E-54
score: 187.3
NoneNo IPR availablePANTHERPTHR45826:SF21BNAA05G20530D PROTEINcoord: 4..453
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 22..401
e-value: 2.3E-31
score: 109.1
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 4..453

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS012187.1MS012187.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity