MS012073 (gene) Bitter gourd (TR) v1

Overview


Name	MS012073
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold708: 79669 .. 81946 (+)
RNA-Seq Expression	MS012073
Synteny	MS012073

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTGTTATGGGAAGAACATGGGAAGCTTATCTCCAATCCCAGTGGGATGATTTTCTAGTTATATCATTCAGCTTTTGATCTCTTTCTTTTTATCCCTTTTTCTCAAACACTCAATTGCAGTCTCCATTTTCTCTATTAAAGGTCAATGCCTTAATGAAGTTGGTCTGTTCCACTGCTATAATAATCAATCCAAAAAGTAACGTTTTTGCACATCAAAGGCTTTGATTCACCTTCCCACTTTCACTTAAAGTTTTCCTTTTCCTCTCATTATATAACAAAAGATTCAGCCTTTCTTGTTTTGTGTTCATTCCATGACAAAATAGGCTATTGGGTTTCAGGAGGAATCCAAACCCCAAGTTTGAGAAAAGTTTGTGGAGAGTTTTATTGATCTTCTTAAATAACCTCTTCACCACCTCTCTTTTTCCTTATATTTGAGTTTTTGATCTGATTTTGGAGCTCAAAAAATGGCTAGTTTTGTGTTGTCACTTGCATTTTGGTGTTCTTTTATTTGTTGCCTTGTTTCTTCTCAAGGTAGATGGCATCATCCCCCCAAGCAAAAGCACTCTCACCACCACAAACATAGCTACATTTCACAGCCACCATCTCTTCCTCCTTCTCCTCCTCCTCCTGATTCACCGGACGAGGGAGATTACGTTAGCGAGATGGATGTTTTCGACGTGAGAGCTTTCGGTGCTATCGGTGACGGCACTGCAGATGACACGGCTGCATTTAAGATGGCATGGGACACGGCCTGCCAAAGCGACGACCCTGCAGTAATTCTCGTTCCCTATAGTTTCTCCTTTATGATTCAATCGACGATCTTCACAGGCCCTTGTCAGGGTCACCTTATGTTACGAGTCGATGGCACATTGATGCCACCAGATGGACCCGATGCCTGGCCAAAAAATACGAGTCATCGACAATGGTTGGTATTTTATAGAATTGATGGAATGTCCCTTGAAGGAGGAGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAGCCACACAAGGTACTCGAAGGTCTTTTTACCATGCGTAGGTAAATGTTTAGAGAATATAAATGAACAATAATTCATGGATATATTATTCTAACTCTCTTAATTTTCATCACGTTAGGGAATAAATGGAACTACGCTACCAGGACCATGTGATAGCCCAATTGTAAGTTCAAGAACCTTACAAAAGTGTTTGCTGATATATAATACTTTTCTGTAAGAAATATGAAGATAAACTTAGACATTGTCACATTGTTATGGTTTAGGCTATAAGGTTCTTCATGAGCTCCAATTTGACTGTGCAAGGACTTAAAATCACAAACAGCCCTCAGTTCCACTTCAGATTCGACGGTTGCAAGAACGTTCATATCGACTCGATTCACATAAAATCTCCCGGTCTTAGTCCGAATACTGATGGTATCCACATCGAGAACACAAATGGCGTCGAGATATATAATTCAGTCATTTACAATGGTCAGTATTGAAACCACAAAAATCTTTTACATTGTTAAGGGAATTATATATATATTCTGATTCATCTCTTTTATGCAGGGGATGATTGTGTATCAATTGGGTCAGGTTCATATGATGTAGATATCAGAAACATCACATGTGGACCAAGTCATGGAATCAGGTATTATACGTTTTATTAGTAGTTCTAAAATATAATTACGACCATAATCTGAATATAATTCTTTTTTCATATATACCAAGTTTCATGTTAATTAATATTGCTTATAAACCTTTTATCCTTCTTTTGGTGTAGCATTGGAAGTCTTGGCGTTCGCAACTCGAGAGCCTGTGTTACGAACATAACGGTTAGAGACTCCGTGATCAAAGTATCAGACAACGGAGTTCGGATCAAGACATGGCAAGGTGGCTATGGGGCAGTAAGAGGAATAACATTTAGCAACATTCACATGGACAATGTTAGGAACCCCATCATTATAGACCAATTTTATTGTCTATCAAAAGATTGCACCAACCAAACCTCAGCTGTTTCAGTATCAGACATTCAGTACTCAAACATCAAAGGAACATACGACATTCGAAGCCCGCCGATGCACCTTGCCTGCAGCGACTCCGTCCCGTGTACCAATCTAACGCTGTCGGAGATCGAGTTGCTTCCTGCTCAGGGAGATATAGTGTTGGACCCTTATTGTTGGAATGCCTATGGAGATCTTCAGACGCTTACTATTCCACCCATCTCTTGCTTGCAAGAGGGGCTTCCTTCTTCTATCAACTTAAACAATGACAATGAGTATTGT

mRNA sequence

ATTGTTATGGGAAGAACATGGGAAGCTTATCTCCAATCCCAGTGGGATGATTTTCTAGTTATATCATTCTTGATCTCTTTCTTTTTATCCCTTTTTCTCAAACACTCAATTGCAGTCTCCATTTTCTCTATTAAAGGTAGATGGCATCATCCCCCCAAGCAAAAGCACTCTCACCACCACAAACATAGCTACATTTCACAGCCACCATCTCTTCCTCCTTCTCCTCCTCCTCCTGATTCACCGGACGAGGGAGATTACGTTAGCGAGATGGATGTTTTCGACGTGAGAGCTTTCGGTGCTATCGGTGACGGCACTGCAGATGACACGGCTGCATTTAAGATGGCATGGGACACGGCCTGCCAAAGCGACGACCCTGCAGTAATTCTCGTTCCCTATAGTTTCTCCTTTATGATTCAATCGACGATCTTCACAGGCCCTTGTCAGGGTCACCTTATGTTACGAGTCGATGGCACATTGATGCCACCAGATGGACCCGATGCCTGGCCAAAAAATACGAGTCATCGACAATGGTTGGTATTTTATAGAATTGATGGAATGTCCCTTGAAGGAGGAGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAGCCACACAAGGGAATAAATGGAACTACGCTACCAGGACCATGTGATAGCCCAATTGCTATAAGGTTCTTCATGAGCTCCAATTTGACTGTGCAAGGACTTAAAATCACAAACAGCCCTCAGTTCCACTTCAGATTCGACGGTTGCAAGAACGTTCATATCGACTCGATTCACATAAAATCTCCCGGTCTTAGTCCGAATACTGATGGTATCCACATCGAGAACACAAATGGCGTCGAGATATATAATTCAGTCATTTACAATGGGGATGATTGTGTATCAATTGGGTCAGGTTCATATGATGTAGATATCAGAAACATCACATGTGGACCAAGTCATGGAATCAGCATTGGAAGTCTTGGCGTTCGCAACTCGAGAGCCTGTGTTACGAACATAACGGTTAGAGACTCCGTGATCAAAGTATCAGACAACGGAGTTCGGATCAAGACATGGCAAGGTGGCTATGGGGCAGTAAGAGGAATAACATTTAGCAACATTCACATGGACAATGTTAGGAACCCCATCATTATAGACCAATTTTATTGTCTATCAAAAGATTGCACCAACCAAACCTCAGCTGTTTCAGTATCAGACATTCAGTACTCAAACATCAAAGGAACATACGACATTCGAAGCCCGCCGATGCACCTTGCCTGCAGCGACTCCGTCCCGTGTACCAATCTAACGCTGTCGGAGATCGAGTTGCTTCCTGCTCAGGGAGATATAGTGTTGGACCCTTATTGTTGGAATGCCTATGGAGATCTTCAGACGCTTACTATTCCACCCATCTCTTGCTTGCAAGAGGGGCTTCCTTCTTCTATCAACTTAAACAATGACAATGAGTATTGT

Coding sequence (CDS)

ATTGTTATGGGAAGAACATGGGAAGCTTATCTCCAATCCCAGTGGGATGATTTTCTAGTTATATCATTCTTGATCTCTTTCTTTTTATCCCTTTTTCTCAAACACTCAATTGCAGTCTCCATTTTCTCTATTAAAGGTAGATGGCATCATCCCCCCAAGCAAAAGCACTCTCACCACCACAAACATAGCTACATTTCACAGCCACCATCTCTTCCTCCTTCTCCTCCTCCTCCTGATTCACCGGACGAGGGAGATTACGTTAGCGAGATGGATGTTTTCGACGTGAGAGCTTTCGGTGCTATCGGTGACGGCACTGCAGATGACACGGCTGCATTTAAGATGGCATGGGACACGGCCTGCCAAAGCGACGACCCTGCAGTAATTCTCGTTCCCTATAGTTTCTCCTTTATGATTCAATCGACGATCTTCACAGGCCCTTGTCAGGGTCACCTTATGTTACGAGTCGATGGCACATTGATGCCACCAGATGGACCCGATGCCTGGCCAAAAAATACGAGTCATCGACAATGGTTGGTATTTTATAGAATTGATGGAATGTCCCTTGAAGGAGGAGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAGCCACACAAGGGAATAAATGGAACTACGCTACCAGGACCATGTGATAGCCCAATTGCTATAAGGTTCTTCATGAGCTCCAATTTGACTGTGCAAGGACTTAAAATCACAAACAGCCCTCAGTTCCACTTCAGATTCGACGGTTGCAAGAACGTTCATATCGACTCGATTCACATAAAATCTCCCGGTCTTAGTCCGAATACTGATGGTATCCACATCGAGAACACAAATGGCGTCGAGATATATAATTCAGTCATTTACAATGGGGATGATTGTGTATCAATTGGGTCAGGTTCATATGATGTAGATATCAGAAACATCACATGTGGACCAAGTCATGGAATCAGCATTGGAAGTCTTGGCGTTCGCAACTCGAGAGCCTGTGTTACGAACATAACGGTTAGAGACTCCGTGATCAAAGTATCAGACAACGGAGTTCGGATCAAGACATGGCAAGGTGGCTATGGGGCAGTAAGAGGAATAACATTTAGCAACATTCACATGGACAATGTTAGGAACCCCATCATTATAGACCAATTTTATTGTCTATCAAAAGATTGCACCAACCAAACCTCAGCTGTTTCAGTATCAGACATTCAGTACTCAAACATCAAAGGAACATACGACATTCGAAGCCCGCCGATGCACCTTGCCTGCAGCGACTCCGTCCCGTGTACCAATCTAACGCTGTCGGAGATCGAGTTGCTTCCTGCTCAGGGAGATATAGTGTTGGACCCTTATTGTTGGAATGCCTATGGAGATCTTCAGACGCTTACTATTCCACCCATCTCTTGCTTGCAAGAGGGGCTTCCTTCTTCTATCAACTTAAACAATGACAATGAGTATTGT

Protein sequence

IVMGRTWEAYLQSQWDDFLVISFLISFFLSLFLKHSIAVSIFSIKGRWHHPPKQKHSHHHKHSYISQPPSLPPSPPPPDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC

Homology

[Close]

BLAST of MS012073 vs. NCBI nr
Match: XP_022146163.1 (polygalacturonase At1g48100 [Momordica charantia])

HSP 1 Score: 942.2 bits (2434), Expect = 1.8e-270
Identity = 448/466 (96.14%), Postives = 452/466 (97.00%), Query Frame = 0


Query: 24  LISFFLSLFLKHSIAVSIFSIKGRWHHPPKQKHSHHHKHSYISQPPSLPPSPPPPDSPDE 83
           + SF LSL    S    + S +GRWHHPPKQKHSHHHKHSYISQPPS PP PPPPDSPDE
Sbjct: 1   MASFVLSLAFWCSFICCLVSSQGRWHHPPKQKHSHHHKHSYISQPPS-PPPPPPPDSPDE 60

Query: 84  GDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIF 143
           GDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIF
Sbjct: 61  GDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIF 120

Query: 144 TGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWD 203
           TGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWD
Sbjct: 121 TGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWD 180

Query: 204 LPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI 263
           LPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI
Sbjct: 181 LPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI 240

Query: 264 HIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI 323
           HIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI
Sbjct: 241 HIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI 300

Query: 324 GSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIII 383
           GSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSN+HMDNVRNPIII
Sbjct: 301 GSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIII 360

Query: 384 DQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLP 443
           DQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLP
Sbjct: 361 DQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLP 420

Query: 444 AQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 490
           AQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC
Sbjct: 421 AQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465

[Close]

BLAST of MS012073 vs. NCBI nr
Match: XP_038899064.1 (polygalacturonase At1g48100-like [Benincasa hispida])

HSP 1 Score: 877.1 bits (2265), Expect = 7.1e-251
Identity = 418/472 (88.56%), Postives = 436/472 (92.37%), Query Frame = 0


Query: 20  VISFLISF-FLSLFLKHSIAVSIFSIKGRWHHPPKQKHSHHHKHSYISQPPSLPPSPPPP 79
           V+SF I F F+S  L         S + RWHH  KQKHSHHHKHSYIS PPSL PSP P 
Sbjct: 5   VLSFAIWFSFISCLLSS-------SSQARWHHHTKQKHSHHHKHSYISHPPSLSPSPSPS 64

Query: 80  DS-PDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFM 139
           DS PD+GDY++E ++FDVRAFGAIG+G  DDT AFKMAWDTACQSDD A++LVPY +SFM
Sbjct: 65  DSPPDDGDYINETNIFDVRAFGAIGNGVVDDTEAFKMAWDTACQSDDSAIMLVPYGYSFM 124

Query: 140 IQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGR 199
           IQSTIFTGPC GHLMLRVDGTLMPPDGPDAWPKN S RQWLVFYRIDGMSLEGGGVIDGR
Sbjct: 125 IQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGR 184

Query: 200 GQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKN 259
           GQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKN
Sbjct: 185 GQKWWDLPCKPHKGRNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKN 244

Query: 260 VHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGP 319
           VHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYD+DIRNITCGP
Sbjct: 245 VHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDLDIRNITCGP 304

Query: 320 SHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNV 379
           SHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSNIHMDNV
Sbjct: 305 SHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNV 364

Query: 380 RNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLS 439
           RNPIIIDQFYCL+KDC NQTSAVSVSDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTLS
Sbjct: 365 RNPIIIDQFYCLTKDCINQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLS 424

Query: 440 EIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 490
           EIELLP+QGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSINLNNDNEYC
Sbjct: 425 EIELLPSQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 469

[Close]

BLAST of MS012073 vs. NCBI nr
Match: TYJ96898.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 863.2 bits (2229), Expect = 1.1e-246
Identity = 413/473 (87.32%), Postives = 436/473 (92.18%), Query Frame = 0


Query: 18  FLVISFLISFFLSLFLKHSIAVSIFSIKGRWHHPPKQKHSHHHKHSYISQPPSLPPSPPP 77
           F+++SF I  +LS      ++  + S + RWHH  KQKHSHHH+HSYIS PPSL  SP P
Sbjct: 4   FVLLSFAI--WLSF-----VSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPSL--SPSP 63

Query: 78  PDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQS-DDPAVILVPYSFSF 137
            DSPD+GDY+++ ++FDVRAFGAIGDG  DDT AFKMAWDTACQS DD AV+LVPY FSF
Sbjct: 64  SDSPDDGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSF 123

Query: 138 MIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDG 197
           MIQSTIFTGPC G+LMLRVDGTLMPPDGPDAWPKN+S RQWLVFYRIDGMSLEGGGVIDG
Sbjct: 124 MIQSTIFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDG 183

Query: 198 RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK 257
           RGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK
Sbjct: 184 RGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCK 243

Query: 258 NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG 317
            VHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYD+DIRNITCG
Sbjct: 244 TVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCG 303

Query: 318 PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDN 377
           PSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSNIHMDN
Sbjct: 304 PSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDN 363

Query: 378 VRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTL 437
           VRNPIIIDQFYCL+KDCTNQTSAVSVSDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTL
Sbjct: 364 VRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTL 423

Query: 438 SEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 490
           SEIELLPAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSIN NNDNEYC
Sbjct: 424 SEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

[Close]

BLAST of MS012073 vs. NCBI nr
Match: XP_008461394.1 (PREDICTED: polygalacturonase At1g48100-like [Cucumis melo])

HSP 1 Score: 861.7 bits (2225), Expect = 3.1e-246
Identity = 412/473 (87.10%), Postives = 435/473 (91.97%), Query Frame = 0


Query: 18  FLVISFLISFFLSLFLKHSIAVSIFSIKGRWHHPPKQKHSHHHKHSYISQPPSLPPSPPP 77
           F+++SF I  +LS      ++  + S + RWHH  KQKHSHHH+H YIS PPSL  SP P
Sbjct: 4   FVLLSFAI--WLSF-----VSCLLSSSQARWHHHTKQKHSHHHRHGYISHPPSL--SPSP 63

Query: 78  PDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQS-DDPAVILVPYSFSF 137
            DSPD+GDY+++ ++FDVRAFGAIGDG  DDT AFKMAWDTACQS DD AV+LVPY FSF
Sbjct: 64  SDSPDDGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSF 123

Query: 138 MIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDG 197
           MIQSTIFTGPC G+LMLRVDGTLMPPDGPDAWPKN+S RQWLVFYRIDGMSLEGGGVIDG
Sbjct: 124 MIQSTIFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDG 183

Query: 198 RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK 257
           RGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK
Sbjct: 184 RGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCK 243

Query: 258 NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG 317
            VHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYD+DIRNITCG
Sbjct: 244 TVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCG 303

Query: 318 PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDN 377
           PSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSNIHMDN
Sbjct: 304 PSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDN 363

Query: 378 VRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTL 437
           VRNPIIIDQFYCL+KDCTNQTSAVSVSDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTL
Sbjct: 364 VRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTL 423

Query: 438 SEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 490
           SEIELLPAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSIN NNDNEYC
Sbjct: 424 SEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

[Close]

BLAST of MS012073 vs. NCBI nr
Match: XP_004135975.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN45046.1 hypothetical protein Csa_016395 [Cucumis sativus])

HSP 1 Score: 850.1 bits (2195), Expect = 9.3e-243
Identity = 407/473 (86.05%), Postives = 431/473 (91.12%), Query Frame = 0


Query: 18  FLVISFLISFFLSLFLKHSIAVSIFSIKGRWHHPPKQKHSHHHKHSYISQPPSLPPSPPP 77
           F+++SF I  +LS      ++  + S + RWHH  KQKHSH H+HSYIS PPSL  SP P
Sbjct: 4   FVLLSFAI--WLSF-----VSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSL--SPSP 63

Query: 78  PDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQS-DDPAVILVPYSFSF 137
            DSPD+G Y+++ ++FDVRAFGAIGDG  DDT AFKMAWDTACQS DD  V+LVPY FSF
Sbjct: 64  SDSPDDGYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSF 123

Query: 138 MIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDG 197
           MIQSTIFTGPC G+LMLRVDGTLM PDGPDAWPKN S RQWLVFYRIDGMSLEGGGVIDG
Sbjct: 124 MIQSTIFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDG 183

Query: 198 RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK 257
           RGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK
Sbjct: 184 RGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCK 243

Query: 258 NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG 317
            VHIDSIHIKSPGLSPNTDGIHIENTNGV+IYNSVI NGDDCVSIGSGSYD+DIRNITCG
Sbjct: 244 TVHIDSIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCG 303

Query: 318 PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDN 377
           PSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSNIHMDN
Sbjct: 304 PSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDN 363

Query: 378 VRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTL 437
           VRNPIIIDQFYCL+KDCTNQTSAVSVSDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTL
Sbjct: 364 VRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTL 423

Query: 438 SEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 490
           SEIELLPAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSS+N NNDNEYC
Sbjct: 424 SEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

[Close]

BLAST of MS012073 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 9.6e-158
Identity = 262/448 (58.48%), Postives = 329/448 (73.44%), Query Frame = 0


Query: 58  HHHKHSYISQPPSLPPSPPPP---------------DSPDEGDYVSEMD-VFDVRAFGAI 117
           H+HK  +    P LPP PPPP                SPD     S+   VFDV +FGA+
Sbjct: 30  HYHKGRHGVTHP-LPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSDSGCVFDVTSFGAV 89

Query: 118 GDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQGHLMLRVDGTLMP 177
           GDG+ DDTAAF+ AW  AC + +  V+L P    F I STIF+GPC+  L+ ++DG LMP
Sbjct: 90  GDGSCDDTAAFQDAWKAAC-AVESGVVLAPEGGVFKITSTIFSGPCKPGLVFQLDGVLMP 149

Query: 178 PDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGINGTTLPGPC 237
           PDGP+ WP+  +  QWLVFYR+DG +  G G ++G GQKWWDLPCKPH+G +G++  GPC
Sbjct: 150 PDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPDGSSSSGPC 209

Query: 238 DSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDGIHIEN 297
            SP  IRFFMS+N+ V+GL+I NSPQFH +FDGC+ V I+ I I SP LSPNTDGIH+ N
Sbjct: 210 ASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPNTDGIHLGN 269

Query: 298 TNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVTNITVR 357
           T  V IYNSV+ NGDDC+SIG+G  DVDI+ +TCGPSHGISIGSLGV NS+ACV+NITVR
Sbjct: 270 TRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQACVSNITVR 329

Query: 358 DSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLSKDCTNQTSAVS 417
           ++VI+ SDNG+R+KTWQGG G+V  + F NI M+NV N II+DQ+YC SKDC N+TSAV 
Sbjct: 330 NTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDCRNETSAVK 389

Query: 418 VSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDIVLDPYCWNAYGDL 477
           V D++Y NIKGTYD+RSPP+H ACSD+V CTN+T+SE+ELLP +G++V DP+CWNAYG  
Sbjct: 390 VFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPFCWNAYGKQ 449

Query: 478 QTLTIPPISCLQEGLPSSINLNNDNEYC 490
           +TLTIPPI CL +G P      + N  C
Sbjct: 450 ETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

[Close]

BLAST of MS012073 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.5e-91
Identity = 166/379 (43.80%), Postives = 231/379 (60.95%), Query Frame = 0


Query: 98  FGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQGHLMLRVDG 157
           FGA G+G  DDT AF  AW TAC S     ILVP +++ +++    +GPC+  L L++ G
Sbjct: 58  FGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARLTLQISG 117

Query: 158 TLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGINGTTL 217
           T++ P+ PD W +  + R+WL F+ +  +++EGGG ++G GQ+WW+  CK H   N    
Sbjct: 118 TIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK-HNHSN---- 177

Query: 218 PGPC-DSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDG 277
             PC  +P A+ F    N+ V+ L + +S Q H     C+ V I  + + +P  SPNTDG
Sbjct: 178 --PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDG 237

Query: 278 IHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVT 337
           IHI  + G+ I N+ +  GDDC+SI   S  + I NI CGP HGISIGSLG   S   V 
Sbjct: 238 IHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVR 297

Query: 338 NITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLS-KDCTN 397
           +ITV  ++I  + NGVRIKTWQGG G V  I F NI M+NV NPIIIDQ+YC S K C N
Sbjct: 298 DITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCAN 357

Query: 398 QTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDIVLDPYCW 457
           QTSA+S+ +I + +++GT       + ++CSDS PC N+ L +I+L P+ GD   + +CW
Sbjct: 358 QTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCW 417

Query: 458 NAYGDLQTLTIPPISCLQE 475
            AYG       PP  CL +
Sbjct: 418 EAYGSSSGQVYPP-PCLSD 426

[Close]

BLAST of MS012073 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.4e-84
Identity = 160/360 (44.44%), Postives = 218/360 (60.56%), Query Frame = 0


Query: 90  MDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQG 149
           +++F+V  +GA+GDG  D T AF  AW  AC+    A++LVP +  F++ +  F GPCQ 
Sbjct: 56  INIFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPS-AMLLVPGNKKFVVNNLFFNGPCQP 115

Query: 150 HLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPH 209
           H   +VDG +     P +W  N   R WL F ++ G +L G GVIDG+G++WW   C   
Sbjct: 116 HFTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQC--- 175

Query: 210 KGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPG 269
           K +NG  +    D P AI+F  S+ L +QGLK+ NSP+FH  F  C+ V I  I I +P 
Sbjct: 176 KWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPR 235

Query: 270 LSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVR 329
            SPNTDGI I  +    +  + I  GDDCV+IG+GS ++ I ++ CGP HGISIGSLG  
Sbjct: 236 DSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRE 295

Query: 330 NSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCL 389
           NSRA V+ + V  +    + NG+RIKTWQGG G    I + N+ M N  NPI+I+QFYC 
Sbjct: 296 NSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCT 355

Query: 390 SKD-CTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDI 449
           S   C NQ SAV + D+ Y NI+GT    +  + L CSDS+PC ++ LS+I L    G I
Sbjct: 356 SASACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

[Close]

BLAST of MS012073 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 5.9e-83
Identity = 161/356 (45.22%), Postives = 218/356 (61.24%), Query Frame = 0


Query: 92  VFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQGHL 151
           VF+V  +GA+GDG  D T AF   W+ AC+    AV+LVP +  F + + +F GPCQ HL
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKKAS-AVLLVPANKKFFVNNLVFRGPCQPHL 117

Query: 152 MLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKG 211
             +VDGT++    P  W KN+  + WL F ++   +L G GVIDG+GQ+WW   CK    
Sbjct: 118 SFKVDGTIVAQPDPARW-KNS--KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV--- 177

Query: 212 INGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLS 271
           +NG T+    + P AI+   S ++TV+ L + NSP+FH  F  C+ V I  + IK+P  S
Sbjct: 178 VNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDS 237

Query: 272 PNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNS 331
           PNTDGI I  +    I   VI  GDDC++IG+GS ++ I+++ CGP HGISIGSLG  NS
Sbjct: 238 PNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNS 297

Query: 332 RACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLSK 391
           RA V+++ V  +    + NG+RIKTWQGG G    IT+ N+ M N  NPI+I+QFYC S 
Sbjct: 298 RAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSA 357

Query: 392 D-CTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQG 447
             C NQ SAV +  + Y NI GT    +  + L CSDSVPCT + LS + L    G
Sbjct: 358 SACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSLKLTSG 405

[Close]

BLAST of MS012073 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 5.0e-82
Identity = 160/358 (44.69%), Postives = 217/358 (60.61%), Query Frame = 0


Query: 92  VFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQGHL 151
           VF+V  +GA+GDG  D T AF+  W+ AC     AV LVP +  F++ + +F GPCQ H 
Sbjct: 59  VFNVEHYGAVGDGKHDSTDAFEKTWNAACNKLS-AVFLVPANKKFVVNNLVFYGPCQPHF 118

Query: 152 MLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKG 211
             +VDGT+     P  W KN+  + W+ F R+   +L G GVIDG+G +WW   CK    
Sbjct: 119 SFKVDGTIAAYPDPAKW-KNS--KIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKT--- 178

Query: 212 INGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLS 271
           ING T+      P AI+   S ++TV+ L +TNSP+FH  F  C  V I  I IK+P  S
Sbjct: 179 INGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRDS 238

Query: 272 PNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNS 331
           PNTDGI I  +   EI    I  GDDCV++G+GS ++ I+++TCGP HG+SIGSLG  NS
Sbjct: 239 PNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGNS 298

Query: 332 RACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLS- 391
           R+ V+ + +  +    + NG+RIKTWQGG G    IT+ N+ M N  NPI+I+QFYC S 
Sbjct: 299 RSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYCTSA 358

Query: 392 KDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDI 449
             C NQ SAV + D+ + NI GT    +  + L CSDSVPC+N+ LS + L    G +
Sbjct: 359 AACKNQRSAVKIQDVTFKNIHGT-SATTAAIQLMCSDSVPCSNIKLSNVFLKLTSGKV 408

[Close]

BLAST of MS012073 vs. ExPASy TrEMBL
Match: A0A6J1CXU8 (polygalacturonase At1g48100 OS=Momordica charantia OX=3673 GN=LOC111015443 PE=3 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 8.7e-271
Identity = 448/466 (96.14%), Postives = 452/466 (97.00%), Query Frame = 0


Query: 24  LISFFLSLFLKHSIAVSIFSIKGRWHHPPKQKHSHHHKHSYISQPPSLPPSPPPPDSPDE 83
           + SF LSL    S    + S +GRWHHPPKQKHSHHHKHSYISQPPS PP PPPPDSPDE
Sbjct: 1   MASFVLSLAFWCSFICCLVSSQGRWHHPPKQKHSHHHKHSYISQPPS-PPPPPPPDSPDE 60

Query: 84  GDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIF 143
           GDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIF
Sbjct: 61  GDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIF 120

Query: 144 TGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWD 203
           TGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWD
Sbjct: 121 TGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWD 180

Query: 204 LPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI 263
           LPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI
Sbjct: 181 LPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI 240

Query: 264 HIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI 323
           HIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI
Sbjct: 241 HIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI 300

Query: 324 GSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIII 383
           GSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSN+HMDNVRNPIII
Sbjct: 301 GSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIII 360

Query: 384 DQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLP 443
           DQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLP
Sbjct: 361 DQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLP 420

Query: 444 AQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 490
           AQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC
Sbjct: 421 AQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465

[Close]

BLAST of MS012073 vs. ExPASy TrEMBL
Match: A0A5D3BFC7 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00080 PE=3 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 5.1e-247
Identity = 413/473 (87.32%), Postives = 436/473 (92.18%), Query Frame = 0


Query: 18  FLVISFLISFFLSLFLKHSIAVSIFSIKGRWHHPPKQKHSHHHKHSYISQPPSLPPSPPP 77
           F+++SF I  +LS      ++  + S + RWHH  KQKHSHHH+HSYIS PPSL  SP P
Sbjct: 4   FVLLSFAI--WLSF-----VSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPSL--SPSP 63

Query: 78  PDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQS-DDPAVILVPYSFSF 137
            DSPD+GDY+++ ++FDVRAFGAIGDG  DDT AFKMAWDTACQS DD AV+LVPY FSF
Sbjct: 64  SDSPDDGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSF 123

Query: 138 MIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDG 197
           MIQSTIFTGPC G+LMLRVDGTLMPPDGPDAWPKN+S RQWLVFYRIDGMSLEGGGVIDG
Sbjct: 124 MIQSTIFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDG 183

Query: 198 RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK 257
           RGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK
Sbjct: 184 RGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCK 243

Query: 258 NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG 317
            VHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYD+DIRNITCG
Sbjct: 244 TVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCG 303

Query: 318 PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDN 377
           PSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSNIHMDN
Sbjct: 304 PSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDN 363

Query: 378 VRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTL 437
           VRNPIIIDQFYCL+KDCTNQTSAVSVSDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTL
Sbjct: 364 VRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTL 423

Query: 438 SEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 490
           SEIELLPAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSIN NNDNEYC
Sbjct: 424 SEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

[Close]

BLAST of MS012073 vs. ExPASy TrEMBL
Match: A0A1S3CFX8 (polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.5e-246
Identity = 412/473 (87.10%), Postives = 435/473 (91.97%), Query Frame = 0


Query: 18  FLVISFLISFFLSLFLKHSIAVSIFSIKGRWHHPPKQKHSHHHKHSYISQPPSLPPSPPP 77
           F+++SF I  +LS      ++  + S + RWHH  KQKHSHHH+H YIS PPSL  SP P
Sbjct: 4   FVLLSFAI--WLSF-----VSCLLSSSQARWHHHTKQKHSHHHRHGYISHPPSL--SPSP 63

Query: 78  PDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQS-DDPAVILVPYSFSF 137
            DSPD+GDY+++ ++FDVRAFGAIGDG  DDT AFKMAWDTACQS DD AV+LVPY FSF
Sbjct: 64  SDSPDDGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSF 123

Query: 138 MIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDG 197
           MIQSTIFTGPC G+LMLRVDGTLMPPDGPDAWPKN+S RQWLVFYRIDGMSLEGGGVIDG
Sbjct: 124 MIQSTIFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDG 183

Query: 198 RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK 257
           RGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK
Sbjct: 184 RGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCK 243

Query: 258 NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG 317
            VHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYD+DIRNITCG
Sbjct: 244 TVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCG 303

Query: 318 PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDN 377
           PSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSNIHMDN
Sbjct: 304 PSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDN 363

Query: 378 VRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTL 437
           VRNPIIIDQFYCL+KDCTNQTSAVSVSDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTL
Sbjct: 364 VRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTL 423

Query: 438 SEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 490
           SEIELLPAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSIN NNDNEYC
Sbjct: 424 SEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

[Close]

BLAST of MS012073 vs. ExPASy TrEMBL
Match: A0A0A0KBA7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414430 PE=3 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 4.5e-243
Identity = 407/473 (86.05%), Postives = 431/473 (91.12%), Query Frame = 0


Query: 18  FLVISFLISFFLSLFLKHSIAVSIFSIKGRWHHPPKQKHSHHHKHSYISQPPSLPPSPPP 77
           F+++SF I  +LS      ++  + S + RWHH  KQKHSH H+HSYIS PPSL  SP P
Sbjct: 4   FVLLSFAI--WLSF-----VSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSL--SPSP 63

Query: 78  PDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQS-DDPAVILVPYSFSF 137
            DSPD+G Y+++ ++FDVRAFGAIGDG  DDT AFKMAWDTACQS DD  V+LVPY FSF
Sbjct: 64  SDSPDDGYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSF 123

Query: 138 MIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDG 197
           MIQSTIFTGPC G+LMLRVDGTLM PDGPDAWPKN S RQWLVFYRIDGMSLEGGGVIDG
Sbjct: 124 MIQSTIFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDG 183

Query: 198 RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK 257
           RGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK
Sbjct: 184 RGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCK 243

Query: 258 NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG 317
            VHIDSIHIKSPGLSPNTDGIHIENTNGV+IYNSVI NGDDCVSIGSGSYD+DIRNITCG
Sbjct: 244 TVHIDSIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCG 303

Query: 318 PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDN 377
           PSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSNIHMDN
Sbjct: 304 PSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDN 363

Query: 378 VRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTL 437
           VRNPIIIDQFYCL+KDCTNQTSAVSVSDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTL
Sbjct: 364 VRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTL 423

Query: 438 SEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 490
           SEIELLPAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSS+N NNDNEYC
Sbjct: 424 SEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

[Close]

BLAST of MS012073 vs. ExPASy TrEMBL
Match: A0A6J1JNI5 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.8e-242
Identity = 401/471 (85.14%), Postives = 426/471 (90.45%), Query Frame = 0


Query: 19  LVISFLISFFLSLFLKHSIAVSIFSIKGRWHHPPKQKHSHHHKHSYISQPPSLPPSPPPP 78
           L +SF ++F   L         + S + RWHH  KQKHSHHHK SYIS PP+  PSP   
Sbjct: 6   LSLSFWVTFLCCL---------LCSSQARWHHHSKQKHSHHHKRSYISHPPT--PSP--- 65

Query: 79  DSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMI 138
            SP +GDY++E ++FDVR FGA+GDG ADDT AFKMAWDTACQSDD AV+LVPY +SFMI
Sbjct: 66  -SPGDGDYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDDAAVVLVPYGYSFMI 125

Query: 139 QSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRG 198
           QSTIFTGPC GHLML VDGTLMPPDGPDAWP+N S RQWLVFYRIDGMSLEGGGV+DGRG
Sbjct: 126 QSTIFTGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRG 185

Query: 199 QKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNV 258
           QKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNV
Sbjct: 186 QKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNV 245

Query: 259 HIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPS 318
           HI+SIHIKSPGLSPNTDGIHIENTN V IYNS+IYNGDDCVSIGSGSYDVDIRNITCGPS
Sbjct: 246 HIESIHIKSPGLSPNTDGIHIENTNDVGIYNSIIYNGDDCVSIGSGSYDVDIRNITCGPS 305

Query: 319 HGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNVR 378
           HGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSNIHMDNVR
Sbjct: 306 HGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVR 365

Query: 379 NPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSE 438
           NPIIIDQFYCL+KDCTNQTSAVS+SDI+Y+NIKGTYDIRS PMHLACSD+VPCTNLTLSE
Sbjct: 366 NPIIIDQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSE 425

Query: 439 IELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 490
           IELLPAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSI LNNDNEYC
Sbjct: 426 IELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

[Close]

BLAST of MS012073 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 642.5 bits (1656), Expect = 2.7e-184
Identity = 298/450 (66.22%), Postives = 362/450 (80.44%), Query Frame = 0


Query: 49  HHPPKQKHSHHHKH--------SYISQPPSLPPSPPP------PDSPDEGDYVSEMDVFD 108
           HH  K KH++H+ H        S ISQPP+ PP PP       P SP +        +++
Sbjct: 31  HHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPSLPPSPSDDPADDNNGIYN 90

Query: 109 VRAFGAIGDGTADDTAAFKMAWDTACQSDD--PAVILVPYSFSFMIQSTIFTGPCQGHLM 168
           VR +GA+GDG  DDT AFK AWD++C +++   +V+LVPY ++FMIQSTIFTGPC+ +  
Sbjct: 91  VRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLVPYGYTFMIQSTIFTGPCRSYQF 150

Query: 169 LRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGI 228
            +VDGT++ PDGP++WP N S RQWLVFYR++GM+L+G GVIDGRGQKWWDLPCKPH+ +
Sbjct: 151 FQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEGVIDGRGQKWWDLPCKPHRSV 210

Query: 229 NGTTL-PGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLS 288
           N + +  GPCDSPIA+RFFMSSNL V+GL+I NSPQFHFRFDGC+ VH++S+HI +P LS
Sbjct: 211 NKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRFDGCQGVHVESLHITAPPLS 270

Query: 289 PNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGP-SHGISIGSLGVRN 348
           PNTDGIHIEN+N V IYNS+I NGDDCVSIGSGSYDVDIRN+TCGP  HGISIGSLG  N
Sbjct: 271 PNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDVDIRNLTCGPGGHGISIGSLGNHN 330

Query: 349 SRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLS 408
           SRACV+NITVRDSVIK SDNGVRIKTWQGG G+V G+TF+NIH+D+VRNPIIIDQ+YC++
Sbjct: 331 SRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVTFNNIHVDSVRNPIIIDQYYCMT 390

Query: 409 KDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDIVL 468
           KDC N+TSAV VSDI Y  IKGTYDIRSPPMH  CSD+VPCTNLTLS+IELLPA+G+IVL
Sbjct: 391 KDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDAVPCTNLTLSDIELLPAKGEIVL 450

Query: 469 DPYCWNAYGDLQTLTIPPISCLQEGLPSSI 481
           DP+CWNAYG  + L+IPP+ CL    P  +
Sbjct: 451 DPFCWNAYGIAEELSIPPVWCLMSDPPKGL 480

[Close]

BLAST of MS012073 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 580.5 bits (1495), Expect = 1.3e-165
Identity = 271/425 (63.76%), Postives = 332/425 (78.12%), Query Frame = 0


Query: 62  HSYISQPPSLPPSP-PPPDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTAC 121
           H   SQPPS  P+P  P  SP     +    V+DVR +GA+G+G ADDT +FK AWD+AC
Sbjct: 33  HHQASQPPSPSPNPNDPSKSPSRSQDLDHEVVYDVRKYGAVGNGVADDTVSFKTAWDSAC 92

Query: 122 ---QSDDPAVILVPYSFSFMIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQW 181
              +++  +V+ VPY F+FMI+STIFTGPC+ +   +VDGT++P DGP +WP + + RQW
Sbjct: 93  SNNKNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSWPSSLNKRQW 152

Query: 182 LVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPH-KGINGTTLPGPCDSPIAIRFFMSSNLT 241
           L FYRI+GM+L+G GVIDGRGQ WWDLPCKPH + +N T L GPC+SP A+RFFMSSN+ 
Sbjct: 153 LAFYRINGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAALRFFMSSNVI 212

Query: 242 VQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGD 301
           V+GL I NSPQ H + DGC  VHI+S+ I SP  SPNTDGIHIEN+N VEIYNSVI NGD
Sbjct: 213 VKGLSIKNSPQVHLKLDGCHVVHINSLRIISPPASPNTDGIHIENSNSVEIYNSVISNGD 272

Query: 302 DCVSIGSGSYDVDIRNITCGP-SHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIK 361
           DCVSIG G+YD+DIRNITCGP  HGISIGSLG +NS ACV+N+TVRDS IK S+NGVRIK
Sbjct: 273 DCVSIGPGAYDIDIRNITCGPGGHGISIGSLGEKNSHACVSNVTVRDSFIKFSENGVRIK 332

Query: 362 TWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYD 421
           TWQGG G+V G+TF NIH+D VRNPIIIDQ+YC +K C N+TSAV V+DI Y +IKGTYD
Sbjct: 333 TWQGGSGSVSGVTFDNIHVDTVRNPIIIDQYYCTTKSCANKTSAVFVNDIVYQSIKGTYD 392

Query: 422 IRSPPMHLACSDSVPCTNLTLSEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEG 481
           IRSPPMH  CS++VPCTNLTLS IELLP++ DIV+ P+CWNAYG     ++P ISCL+  
Sbjct: 393 IRSPPMHFGCSNNVPCTNLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSVPLISCLKSN 452

[Close]

BLAST of MS012073 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 558.1 bits (1437), Expect = 6.8e-159
Identity = 262/448 (58.48%), Postives = 329/448 (73.44%), Query Frame = 0


Query: 58  HHHKHSYISQPPSLPPSPPPP---------------DSPDEGDYVSEMD-VFDVRAFGAI 117
           H+HK  +    P LPP PPPP                SPD     S+   VFDV +FGA+
Sbjct: 30  HYHKGRHGVTHP-LPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSDSGCVFDVTSFGAV 89

Query: 118 GDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQGHLMLRVDGTLMP 177
           GDG+ DDTAAF+ AW  AC + +  V+L P    F I STIF+GPC+  L+ ++DG LMP
Sbjct: 90  GDGSCDDTAAFQDAWKAAC-AVESGVVLAPEGGVFKITSTIFSGPCKPGLVFQLDGVLMP 149

Query: 178 PDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGINGTTLPGPC 237
           PDGP+ WP+  +  QWLVFYR+DG +  G G ++G GQKWWDLPCKPH+G +G++  GPC
Sbjct: 150 PDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPDGSSSSGPC 209

Query: 238 DSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDGIHIEN 297
            SP  IRFFMS+N+ V+GL+I NSPQFH +FDGC+ V I+ I I SP LSPNTDGIH+ N
Sbjct: 210 ASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPNTDGIHLGN 269

Query: 298 TNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVTNITVR 357
           T  V IYNSV+ NGDDC+SIG+G  DVDI+ +TCGPSHGISIGSLGV NS+ACV+NITVR
Sbjct: 270 TRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQACVSNITVR 329

Query: 358 DSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLSKDCTNQTSAVS 417
           ++VI+ SDNG+R+KTWQGG G+V  + F NI M+NV N II+DQ+YC SKDC N+TSAV 
Sbjct: 330 NTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDCRNETSAVK 389

Query: 418 VSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDIVLDPYCWNAYGDL 477
           V D++Y NIKGTYD+RSPP+H ACSD+V CTN+T+SE+ELLP +G++V DP+CWNAYG  
Sbjct: 390 VFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPFCWNAYGKQ 449

Query: 478 QTLTIPPISCLQEGLPSSINLNNDNEYC 490
           +TLTIPPI CL +G P      + N  C
Sbjct: 450 ETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

[Close]

BLAST of MS012073 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 554.7 bits (1428), Expect = 7.5e-158
Identity = 253/415 (60.96%), Postives = 330/415 (79.52%), Query Frame = 0


Query: 65  ISQPPSLPPSPPPPDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDD 124
           I+Q   L PSP P  + ++ D ++   VFDV +FGAIGD + DDT+AFKMAWD AC S  
Sbjct: 21  IAQSIYLSPSPAPNPAYNDNDNIAP-TVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTG 80

Query: 125 P--AVILVPYSFSFMIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYR 184
           P  A++LVPY+F F+++ T F GPC+ +L+L++DG ++ PDGP +WP N   RQW++FYR
Sbjct: 81  PKSALLLVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSN-YQRQWMMFYR 140

Query: 185 IDGMSLEGGGVIDGRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKI 244
           ++G+S++G GVI+GRGQKWW+LPCKPHKG+NGTT  GPCDSP+AIR F SS + +QG+  
Sbjct: 141 VNGLSIQGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINF 200

Query: 245 TNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIG 304
            NS QFH RFD C +V +DS+ IK+P  SPNTDGIHIENT+ V+I NS+I NGDDC+SIG
Sbjct: 201 MNSAQFHVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIG 260

Query: 305 SGSYDVDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYG 364
           +G ++VDI+N+TCGPSHGISIGSLGV NS+A V+NITV +S I  SDNGVRIKTWQGG G
Sbjct: 261 AGCFNVDIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSG 320

Query: 365 AVRGITFSNIHMDNVRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMH 424
           +V  I FSNI M NVRNPI+IDQ+YC + +C NQTSAV +SD+ Y+NIKGTYD+RSPP+H
Sbjct: 321 SVSRIVFSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIH 380

Query: 425 LACSDSVPCTNLTLSEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLP 478
             CSDSVPCTNLTL+E++L P++G  + +P+CWNAYG ++ +T+PP+ CL +  P
Sbjct: 381 FGCSDSVPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

[Close]

BLAST of MS012073 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 378.6 bits (971), Expect = 7.4e-105
Identity = 192/454 (42.29%), Postives = 278/454 (61.23%), Query Frame = 0


Query: 65  ISQPPSLPPSPP----PPDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTAC 124
           +SQPP +   PP    PP +P     V +   F+V  FGA GDG +DDT AF+ AW +AC
Sbjct: 91  VSQPPQVQQPPPPHVQPPPTPLPLQPVEDSQQFNVLDFGAKGDGMSDDTQAFEAAWASAC 150

Query: 125 QSDDPAVILVPYSFSFMIQSTIFTGP-CQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLV 184
           +  + + +++P  + F++    F+GP CQ +++ +++G ++ P   ++W        W+ 
Sbjct: 151 KV-EASTMIIPPDYIFLVGPISFSGPYCQANIVFQLEGMIVAPTDTESW--GGGLMWWIE 210

Query: 185 FYRIDGMSLEGGGVIDGRGQKWW------DLPC----------------KPHKGING-TT 244
           F ++ G++++G GVIDGRG  WW      D P                 +P   I     
Sbjct: 211 FTKLSGITIQGNGVIDGRGTVWWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELN 270

Query: 245 LPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDG 304
              P   P A+RF+ S ++TV G+ I NSPQ H +FD C  V +  +++ SPG SPNTDG
Sbjct: 271 WRMPSIKPTALRFYGSIDVTVTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDG 330

Query: 305 IHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVT 364
           IH++NT  V I+ + +  GDDC+SI +G  +V + N+ CGP HGISIGSLG  +++ACV+
Sbjct: 331 IHLQNTRDVMIHTTTLACGDDCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVS 390

Query: 365 NITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLSKDCTNQ 424
           NITVRD V+  +  GVRIKTWQGG G+V+GI FSNI +  V+ PI+IDQFYC    C N 
Sbjct: 391 NITVRDVVMHNTMTGVRIKTWQGGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMNH 450

Query: 425 TSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDI-VLDPYCW 484
           TSAVSV  + Y  I+GTY ++  P+H ACSDS PC ++ LS IEL P Q    + DP+CW
Sbjct: 451 TSAVSVEGVTYEKIRGTYTVK--PVHFACSDSFPCIDVQLSGIELKPVQLQYHMYDPFCW 510

Query: 485 NAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 490
             +G+L + T+PPI CLQ G P+   +++D++ C
Sbjct: 511 KTFGELNSATVPPIDCLQIGKPARNGVHSDHDIC 539

The following BLAST results are available for this feature:

BLAST of MS012073 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146163.1	1.8e-270	96.14	polygalacturonase At1g48100 [Momordica charantia]	[more]
XP_038899064.1	7.1e-251	88.56	polygalacturonase At1g48100-like [Benincasa hispida]	[more]
TYJ96898.1	1.1e-246	87.32	polygalacturonase [Cucumis melo var. makuwa]	[more]
XP_008461394.1	3.1e-246	87.10	PREDICTED: polygalacturonase At1g48100-like [Cucumis melo]	[more]
XP_004135975.1	9.3e-243	86.05	polygalacturonase At1g48100 [Cucumis sativus] >KGN45046.1 hypothetical protein C...	[more]

BLAST of MS012073 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949Z1	9.6e-158	58.48	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
Q94AJ5	3.5e-91	43.80	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
P43212	1.4e-84	44.44	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]
Q7M1E7	5.9e-83	45.22	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
Q9FY19	5.0e-82	44.69	Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1	[more]

BLAST of MS012073 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXU8	8.7e-271	96.14	polygalacturonase At1g48100 OS=Momordica charantia OX=3673 GN=LOC111015443 PE=3 ...	[more]
A0A5D3BFC7	5.1e-247	87.32	Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00...	[more]
A0A1S3CFX8	1.5e-246	87.10	polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV...	[more]
A0A0A0KBA7	4.5e-243	86.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414430 PE=3 SV=1	[more]
A0A6J1JNI5	3.8e-242	85.14	polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=...	[more]

BLAST of MS012073 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02460.1	2.7e-184	66.22	Pectin lyase-like superfamily protein	[more]
AT4G01890.1	1.3e-165	63.76	Pectin lyase-like superfamily protein	[more]
AT1G48100.1	6.8e-159	58.48	Pectin lyase-like superfamily protein	[more]
AT1G56710.1	7.5e-158	60.96	Pectin lyase-like superfamily protein	[more]
AT1G60590.1	7.4e-105	42.29	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 282..303 e-value: 90.0 score: 11.9 coord: 364..385 e-value: 710.0 score: 8.3 coord: 305..325 e-value: 6600.0 score: 1.4 coord: 255..281 e-value: 94.0 score: 11.9 coord: 335..356 e-value: 14.0 score: 14.6
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 86..478 e-value: 1.1E-120 score: 405.3
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 120..441 e-value: 1.8E-89 score: 300.0
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 312..325
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 68..82
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 52..85
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 54..478
None	No IPR available	PANTHER	PTHR31375:SF119	POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEIN	coord: 54..478
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 91..481

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012073.1	MS012073.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity