MS011743 (gene) Bitter gourd (TR) v1

Overview


Name	MS011743
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	cucumber peeling cupredoxin-like
Location	scaffold11: 330402 .. 330620 (-)
RNA-Seq Expression	MS011743
Synteny	MS011743

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CATAGTGTGCTTGAAGTAACAAAGGATGGGTTTGAAAATTGTACCAGTTCAGGAACACCTAATGGCACCAGCCCTGTGATAATTCCTCTGGAGACTGTTGGCTCTAAATACTTTATCTGCACGGTCGGTCCCCACTGCTCGTTGGGGCAGAAGTTGAGCGTGACTGTTGGATCTAAGAACTCGGCACCTCCAGCAGCACCAACAACACCTGCAACACCA

mRNA sequence

CATAGTGTGCTTGAAGTAACAAAGGATGGGTTTGAAAATTGTACCAGTTCAGGAACACCTAATGGCACCAGCCCTGTGATAATTCCTCTGGAGACTGTTGGCTCTAAATACTTTATCTGCACGGTCGGTCCCCACTGCTCGTTGGGGCAGAAGTTGAGCGTGACTGTTGGATCTAAGAACTCGGCACCTCCAGCAGCACCAACAACACCTGCAACACCA

Coding sequence (CDS)

CATAGTGTGCTTGAAGTAACAAAGGATGGGTTTGAAAATTGTACCAGTTCAGGAACACCTAATGGCACCAGCCCTGTGATAATTCCTCTGGAGACTGTTGGCTCTAAATACTTTATCTGCACGGTCGGTCCCCACTGCTCGTTGGGGCAGAAGTTGAGCGTGACTGTTGGATCTAAGAACTCGGCACCTCCAGCAGCACCAACAACACCTGCAACACCA

Protein sequence

HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKNSAPPAAPTTPATP

Homology

[Close]

BLAST of MS011743 vs. NCBI nr
Match: XP_022143139.1 (umecyanin-like [Momordica charantia])

HSP 1 Score: 151.0 bits (380), Expect = 4.0e-33
Identity = 73/73 (100.00%), Postives = 73/73 (100.00%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN 60
           HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN
Sbjct: 98  HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN 157

Query: 61  SAPPAAPTTPATP 74
           SAPPAAPTTPATP
Sbjct: 158 SAPPAAPTTPATP 170

[Close]

BLAST of MS011743 vs. NCBI nr
Match: VFQ64268.1 (unnamed protein product [Cuscuta campestris])

HSP 1 Score: 76.6 bits (187), Expect = 9.6e-11
Identity = 33/72 (45.83%), Postives = 48/72 (66.67%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN 60
           H+V EV++  F +C+++   N T PV I + T G  Y+ICTVGPHCSLGQKL++ V  + 
Sbjct: 90  HNVAEVSRSSFNSCSTTAAANTTGPVTIRIRTTGEHYYICTVGPHCSLGQKLAINVSGEA 149

Query: 61  SAPPAAPTTPAT 73
           + P A P + A+
Sbjct: 150 AGPAATPPSAAS 161

[Close]

BLAST of MS011743 vs. NCBI nr
Match: RAL50935.1 (hypothetical protein DM860_005291 [Cuscuta australis])

HSP 1 Score: 76.3 bits (186), Expect = 1.3e-10
Identity = 32/70 (45.71%), Postives = 47/70 (67.14%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN 60
           H+V EV++  F +C+++   N T PV I ++T G  Y+ICTVGPHC LGQKL++ V    
Sbjct: 85  HNVAEVSRTSFNSCSTTAAANTTGPVTIRIKTTGEHYYICTVGPHCLLGQKLAINVSGGT 144

Query: 61  SAPPAAPTTP 71
           ++P + P TP
Sbjct: 145 ASPVSRPATP 154

[Close]

BLAST of MS011743 vs. NCBI nr
Match: VFQ61189.1 (unnamed protein product [Cuscuta campestris])

HSP 1 Score: 75.9 bits (185), Expect = 1.6e-10
Identity = 32/70 (45.71%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 1  HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN 60
          H+V EV++  F +C+++   N T PV I + T G  Y+ICTVGPHC LGQKL++ V    
Sbjct: 4  HNVAEVSRTSFNSCSTTAAANTTGPVTIRIRTTGEHYYICTVGPHCLLGQKLAINVSGGT 63

Query: 61 SAPPAAPTTP 71
          ++P + P TP
Sbjct: 64 ASPVSRPATP 73

[Close]

BLAST of MS011743 vs. NCBI nr
Match: XP_018830411.1 (cucumber peeling cupredoxin-like [Juglans regia] >KAF5466752.1 hypothetical protein F2P56_016652 [Juglans regia])

HSP 1 Score: 75.5 bits (184), Expect = 2.1e-10
Identity = 42/84 (50.00%), Postives = 52/84 (61.90%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSS---GTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVG 60
           H VL+V K+ +++C+SS   G    T PV I L T G+ Y+ICT+G HC  GQKLSVTV 
Sbjct: 71  HDVLQVPKESYDSCSSSNPIGDTITTGPVNITLSTAGTHYYICTLGRHCLSGQKLSVTVS 130

Query: 61  S--------KNSAPPAAPTTPATP 74
           S          S PP+ PTTPATP
Sbjct: 131 SSPSGVPPTSMSTPPSTPTTPATP 154

[Close]

BLAST of MS011743 vs. ExPASy Swiss-Prot
Match: P42849 (Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.5e-09
Identity = 34/72 (47.22%), Postives = 41/72 (56.94%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSSG--TPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTV-- 60
           H V  VTKD F+NC      +   T PV I L T G +Y+ICTVG HC +GQKLS+ V  
Sbjct: 44  HDVAVVTKDAFDNCKKENPISHMTTPPVKIMLNTTGPQYYICTVGDHCRVGQKLSINVVG 103

Query: 61  --GSKNSAPPAA 67
             G+   A P A
Sbjct: 104 AGGAGGGATPGA 115

[Close]

BLAST of MS011743 vs. ExPASy Swiss-Prot
Match: Q07488 (Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 3.2e-09
Identity = 36/76 (47.37%), Postives = 43/76 (56.58%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSSGTPNGTS--PVIIPLETVGSKYFICTVGPHCSLGQKLSVTV-- 60
           H V  V++  FENC      +  +  PV I L T G +YFICTVG HC  GQKLS+TV  
Sbjct: 66  HDVAVVSEAAFENCEKEKPISHMTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLSITVVA 125

Query: 61  -GSKNSAPPAAPTTPA 72
            G+   A P A  TPA
Sbjct: 126 AGATGGATPGAGATPA 141

[Close]

BLAST of MS011743 vs. ExPASy Swiss-Prot
Match: P29602 (Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3)

HSP 1 Score: 58.5 bits (140), Expect = 3.6e-08
Identity = 35/79 (44.30%), Postives = 48/79 (60.76%), Query Frame = 0


Query: 1   HSVLEV-TKDGFENCTSSGTPNG---TSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTV 60
           H+V E+ TK  F+ C    + N    TSPVI  L+ +G  YF+CTVG HCS GQKLS+ V
Sbjct: 46  HNVHEMETKQSFDACNFVNSDNDVERTSPVIERLDELGMHYFVCTVGTHCSNGQKLSINV 105

Query: 61  GSKN---SAPPAAPTTPAT 73
            + N   S PP + + P++
Sbjct: 106 VAANATVSMPPPSSSPPSS 124

[Close]

BLAST of MS011743 vs. ExPASy Swiss-Prot
Match: O82081 (Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.3e-06
Identity = 31/77 (40.26%), Postives = 40/77 (51.95%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSSGT----PNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTV 60
           H V+EVTK  F++C +        NG S  ++PL T G +YFIC +  HCS G KL V V
Sbjct: 64  HDVVEVTKPEFDSCQAVKPLITFANGNS--LVPLTTPGKRYFICGMPGHCSQGMKLEVNV 123

Query: 61  GSKNSAPPAAPTTPATP 74
               +  P AP     P
Sbjct: 124 VPTATVAPTAPLPNTVP 138

[Close]

BLAST of MS011743 vs. ExPASy Swiss-Prot
Match: O80517 (Uclacyanin-2 OS=Arabidopsis thaliana OX=3702 GN=At2g44790 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 7.4e-06
Identity = 35/81 (43.21%), Postives = 44/81 (54.32%), Query Frame = 0


Query: 1   HSVLEVTKDGFENC-TSSGTPN-GTSPVIIPLETVGSKYFICTVGPHCSL--GQKLSVTV 60
           H+V  V K G++ C  SS T N       I L+TVG  YFIC+   HC    G KL+V V
Sbjct: 65  HTVDVVDKAGYDGCDASSSTENHSDGDTKIDLKTVGINYFICSTPGHCRTNGGMKLAVNV 124

Query: 61  GSKNSAPPAAPT----TPATP 74
            + ++ PPA PT    TP TP
Sbjct: 125 VAGSAGPPATPTPPSSTPGTP 145

[Close]

BLAST of MS011743 vs. ExPASy TrEMBL
Match: A0A6J1CNF9 (umecyanin-like OS=Momordica charantia OX=3673 GN=LOC111013077 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.9e-33
Identity = 73/73 (100.00%), Postives = 73/73 (100.00%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN 60
           HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN
Sbjct: 98  HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN 157

Query: 61  SAPPAAPTTPATP 74
           SAPPAAPTTPATP
Sbjct: 158 SAPPAAPTTPATP 170

[Close]

BLAST of MS011743 vs. ExPASy TrEMBL
Match: A0A484KHC5 (Phytocyanin domain-containing protein OS=Cuscuta campestris OX=132261 GN=CCAM_LOCUS6044 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 4.7e-11
Identity = 33/72 (45.83%), Postives = 48/72 (66.67%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN 60
           H+V EV++  F +C+++   N T PV I + T G  Y+ICTVGPHCSLGQKL++ V  + 
Sbjct: 90  HNVAEVSRSSFNSCSTTAAANTTGPVTIRIRTTGEHYYICTVGPHCSLGQKLAINVSGEA 149

Query: 61  SAPPAAPTTPAT 73
           + P A P + A+
Sbjct: 150 AGPAATPPSAAS 161

[Close]

BLAST of MS011743 vs. ExPASy TrEMBL
Match: A0A328DYY1 (Phytocyanin domain-containing protein OS=Cuscuta australis OX=267555 GN=DM860_005291 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 6.1e-11
Identity = 32/70 (45.71%), Postives = 47/70 (67.14%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN 60
           H+V EV++  F +C+++   N T PV I ++T G  Y+ICTVGPHC LGQKL++ V    
Sbjct: 85  HNVAEVSRTSFNSCSTTAAANTTGPVTIRIKTTGEHYYICTVGPHCLLGQKLAINVSGGT 144

Query: 61  SAPPAAPTTP 71
           ++P + P TP
Sbjct: 145 ASPVSRPATP 154

[Close]

BLAST of MS011743 vs. ExPASy TrEMBL
Match: A0A484KE05 (Phytocyanin domain-containing protein OS=Cuscuta campestris OX=132261 GN=CCAM_LOCUS2965 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 8.0e-11
Identity = 32/70 (45.71%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 1  HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN 60
          H+V EV++  F +C+++   N T PV I + T G  Y+ICTVGPHC LGQKL++ V    
Sbjct: 4  HNVAEVSRTSFNSCSTTAAANTTGPVTIRIRTTGEHYYICTVGPHCLLGQKLAINVSGGT 63

Query: 61 SAPPAAPTTP 71
          ++P + P TP
Sbjct: 64 ASPVSRPATP 73

[Close]

BLAST of MS011743 vs. ExPASy TrEMBL
Match: A0A2I4FFI8 (cucumber peeling cupredoxin-like OS=Juglans regia OX=51240 GN=LOC108998338 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.0e-10
Identity = 42/84 (50.00%), Postives = 52/84 (61.90%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSS---GTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVG 60
           H VL+V K+ +++C+SS   G    T PV I L T G+ Y+ICT+G HC  GQKLSVTV 
Sbjct: 71  HDVLQVPKESYDSCSSSNPIGDTITTGPVNITLSTAGTHYYICTLGRHCLSGQKLSVTVS 130

Query: 61  S--------KNSAPPAAPTTPATP 74
           S          S PP+ PTTPATP
Sbjct: 131 SSPSGVPPTSMSTPPSTPTTPATP 154

[Close]

BLAST of MS011743 vs. TAIR 10
Match: AT5G20230.1 (blue-copper-binding protein )

HSP 1 Score: 62.0 bits (149), Expect = 2.3e-10
Identity = 36/76 (47.37%), Postives = 43/76 (56.58%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSSGTPNGTS--PVIIPLETVGSKYFICTVGPHCSLGQKLSVTV-- 60
           H V  V++  FENC      +  +  PV I L T G +YFICTVG HC  GQKLS+TV  
Sbjct: 66  HDVAVVSEAAFENCEKEKPISHMTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLSITVVA 125

Query: 61  -GSKNSAPPAAPTTPA 72
            G+   A P A  TPA
Sbjct: 126 AGATGGATPGAGATPA 141

[Close]

BLAST of MS011743 vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 52.0 bits (123), Expect = 2.4e-07
Identity = 31/77 (40.26%), Postives = 40/77 (51.95%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCTSSGT----PNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTV 60
           H V+EVTK  F++C +        NG S  ++PL T G +YFIC +  HCS G KL V V
Sbjct: 64  HDVVEVTKPEFDSCQAVKPLITFANGNS--LVPLTTPGKRYFICGMPGHCSQGMKLEVNV 123

Query: 61  GSKNSAPPAAPTTPATP 74
               +  P AP     P
Sbjct: 124 VPTATVAPTAPLPNTVP 138

[Close]

BLAST of MS011743 vs. TAIR 10
Match: AT2G44790.1 (uclacyanin 2 )

HSP 1 Score: 50.8 bits (120), Expect = 5.3e-07
Identity = 35/81 (43.21%), Postives = 44/81 (54.32%), Query Frame = 0


Query: 1   HSVLEVTKDGFENC-TSSGTPN-GTSPVIIPLETVGSKYFICTVGPHCSL--GQKLSVTV 60
           H+V  V K G++ C  SS T N       I L+TVG  YFIC+   HC    G KL+V V
Sbjct: 65  HTVDVVDKAGYDGCDASSSTENHSDGDTKIDLKTVGINYFICSTPGHCRTNGGMKLAVNV 124

Query: 61  GSKNSAPPAAPT----TPATP 74
            + ++ PPA PT    TP TP
Sbjct: 125 VAGSAGPPATPTPPSSTPGTP 145

[Close]

BLAST of MS011743 vs. TAIR 10
Match: AT1G72230.1 (Cupredoxin superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 2.9e-05
Identity = 23/63 (36.51%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 1   HSVLEVTKDGFENCT--SSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGS 60
           H+V EV++  +++CT  ++ + + +    I L+T G  YFIC +  HC+ G KLSV V +
Sbjct: 60  HTVDEVSESDYKSCTLGNAISSDSSGTTSIALKTPGPHYFICGIPGHCTGGMKLSVIVPA 119

Query: 61  KNS 62
            +S
Sbjct: 120 ASS 122

The following BLAST results are available for this feature:

BLAST of MS011743 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143139.1	4.0e-33	100.00	umecyanin-like [Momordica charantia]	[more]
VFQ64268.1	9.6e-11	45.83	unnamed protein product [Cuscuta campestris]	[more]
RAL50935.1	1.3e-10	45.71	hypothetical protein DM860_005291 [Cuscuta australis]	[more]
VFQ61189.1	1.6e-10	45.71	unnamed protein product [Cuscuta campestris]	[more]
XP_018830411.1	2.1e-10	50.00	cucumber peeling cupredoxin-like [Juglans regia] >KAF5466752.1 hypothetical prot...	[more]

BLAST of MS011743 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42849	2.5e-09	47.22	Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1	[more]
Q07488	3.2e-09	47.37	Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2	[more]
P29602	3.6e-08	44.30	Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3	[more]
O82081	3.3e-06	40.26	Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1	[more]
O80517	7.4e-06	43.21	Uclacyanin-2 OS=Arabidopsis thaliana OX=3702 GN=At2g44790 PE=2 SV=1	[more]

BLAST of MS011743 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CNF9	1.9e-33	100.00	umecyanin-like OS=Momordica charantia OX=3673 GN=LOC111013077 PE=4 SV=1	[more]
A0A484KHC5	4.7e-11	45.83	Phytocyanin domain-containing protein OS=Cuscuta campestris OX=132261 GN=CCAM_LO...	[more]
A0A328DYY1	6.1e-11	45.71	Phytocyanin domain-containing protein OS=Cuscuta australis OX=267555 GN=DM860_00...	[more]
A0A484KE05	8.0e-11	45.71	Phytocyanin domain-containing protein OS=Cuscuta campestris OX=132261 GN=CCAM_LO...	[more]
A0A2I4FFI8	1.0e-10	50.00	cucumber peeling cupredoxin-like OS=Juglans regia OX=51240 GN=LOC108998338 PE=4 ...	[more]

BLAST of MS011743 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20230.1	2.3e-10	47.37	blue-copper-binding protein	[more]
AT2G32300.1	2.4e-07	40.26	uclacyanin 1	[more]
AT2G44790.1	5.3e-07	43.21	uclacyanin 2	[more]
AT1G72230.1	2.9e-05	36.51	Cupredoxin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 1..50 e-value: 7.6E-9 score: 35.5
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 1..58 score: 21.451101
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 1..68 e-value: 1.8E-12 score: 49.3
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 1..56
None	No IPR available	PANTHER	PTHR33021:SF360	OS08G0482600 PROTEIN	coord: 1..63
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 1..63
IPR028871	Blue (type 1) copper protein, binding site	PROSITE	PS00196	COPPER_BLUE	coord: 34..50

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS011743.1	MS011743.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0009055	electron transfer activity