MS011629 (gene) Bitter gourd (TR) v1

Overview
NameMS011629
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAsparagine synthetase [glutamine-hydrolyzing]
Locationscaffold239: 3373043 .. 3378124 (+)
RNA-Seq ExpressionMS011629
SyntenyMS011629
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTGGAATCCTCGCAGTTTTAGGCTGCGCCGACGGCTCTCAGGCTAAGCGTTCCCGAATCATCGAATTGTCTCGGAGGTCATTTTCCTCACATTTCTTTCTCTGTAATCGCCTTGGTATTTTGACATTGATGCCAATGGAAAATAAGCGTTATGAATGTTTGACAATATAAAAGCGCCGCATTTGTTTTATGATTCCTTTTGTTGTTTGTTCTCTTCCCTCTTCGTCTGAAGTGAAACAGTGGATTATTTTATATTAATTTGTGGGGTTTTAATCTGTTTGGAACTGCAGGTTGCGCCATAGAGGTCCCGATTGGAGTGGTTTGCATTGTTTTGAGAACTGTTATCTTGCTCATCAAAGATTGGCTATTGTAGACCCCACTTCCGGGGATCAACCCCTTTACAACGAAGACAAGACCGTCGTTGTTACAGTAAGGATAAATGGATGCCATTTCTGCAAATATACTTTCTCATATCATTTAATTATGCCGTCATGTCGTTTAATTTTCATTGCTTTTGTTTCTGGTTTCTGAAGAAAAAACTAAGCTTGTTTCAAACATTTGGCAGAAAGACCTGATAATCAGCATCAAGTTTGGTCAAAGTGTGTGAGAATTGATCAAGAAATATTGTTTTTTTATTGTGCACTTTACCGAGTCTGGATAAAATTTACCATATACCGTTTGTCATTTTTAATGATAGTGTGAACCACCAATACAAGTTTATTTGTGGGCATATACATGGTTTGCTTAAAATTTAATGTGTTATAAATTGTTCTTCAATCTTTAATACTTTTTACTGTGTATATAATTGATGATATGATGCTCCCCTTATTTCTCAATGAAGGAACTATTAGGTTTTCCTTAGTACCGAGATATGTTTTGCAATCTTAAATTAGGCTCACTTGTTTATTACGTTTAAATATTGTTTTGTTTAAGACTTTTGATAGAAGCATTTTTTGGTTCCTAGATGGCATACTCTATCTTAAGATTGTGCTGTCTGTATTTACTCTATGTTAGTAACTAATAACTTAGTTAAATTTGAATTTTGAACCATGTACTAGGTTAACGGGGAGATCTACAACCACAAGGAATTGAGAGAAAAGCTCAAGGGTCATCAGTTCCGAACTGGCAGTGACTGTGAAGTGATTGCACACCTTGTTAGTAGCCAAGAACTTACTGAAATAATTTTTCCATGCACCTATGCGTTCCTGCTATTAGTTTGTTCTGTTCTTAACTTAATACTTATCTTTTCCAGTACGAAGAATATGGAGAAGAGTTTGTGGACATGTTGGATGGCATGTTTTCCTTCGTCCTTCTTGATACTCGTAATAAAACTTTTATTGCAGCCAGGGATGCTATTGGGATCACCCCACTATATATGGGATGGGGTCTTGATGGTATGTAATTTCACATTGTATATGTGACTCACAACCTCAAGTTGTAGTGAGAAAACTCTCCAATTGATAATTAGTGTTCATTTATACGTATTACTGCTTACATTTTTACTGATTGTCGATTGATCTCTGAAAATATATGGCTGCTACCTTCTTGCCAGGATCAATCTGGTTTGCTTCTGAAATGAAAGCTCTAAGTGATGATTGCGAGAGATTCATTTCTTTTCTTCCTGGGCATATATATTCTAGCAAAGAAGGTATGCATCTTTTGTTTTCCCGAATCATATGGTTTACTCTACTTTTTTCCAGTTTTCACGAAGAGTAATGTTATAATCCATGAGATTCGTTACTAAATGGTGGATTTTAAATGTTTTTAGGAGGGTTGAGAAGATGGTATAACCCTCCGTGGTACACAGAGATGGTACCATCAGGTCCATATGATCCTTTGGTTCTACGTAAGGCATTTGAGAAGGCATGTTCTGGACTTACACTTATTAGTTTAACATACTATGTCATCTTCTTCGAGGAGTTTTATTTAACACAGTAGTTTTTCAGTTTTAATGTGATGAGATTGCTACCATTGTTAGTGTTTGATGTTTACTGTTTTACAAGGACTTTTATATGAAAACCTGCTGTCTTGCTTGATTGACGAATCATGCAATGTCTCATATGCTAAGATTTTCTGATTTTAAGCTTTCCTCTATATTTTTATGGTGTAGGCTGTTACTAAGAGACTTATGACAGATGTGCCCTTTGGGGTCCTTTTATCTGGAGGATTGGATTCATCACTAGTAGCTGCTGTTGCCTGCCGCCATTTGGCTGATTCTGAAGCAGCTCGTCAGTGGGGGTCACAATTACACACCTTTTGCGTTGGCTTGAAGGTTAGATAATGAGGGAATTAAGCATATATTTTACTTGAAATCGTAAAACATTTTCATCGAAGGGTAACCCTCGGCCCTCGGTAATAGGTTTCTTCTGTTACATTCTTTTTAACATTGTGGTTGGTTTATTTCTTTGCAAAATTTAATTATTTTTTATAACAAATATGTTGTTTGATGAACGAATGAAATGCTCTACTATTGACATATAATTTTAGTAAATTTTTACATGTAATCATTGAAAGATGAAATTACTTTAAATTAGTGGTTGGAAGCTGTTTAAAGAGCATGCCCCCCGAGTGTCTCTACCAGCATATCTGGATTATTGTCATAATCTTAACTTAAGACATGCCTTCATTTTAACTGTTCTCTACCAGCATAATTTTACTTGGATATGGGGACTTAATGGGACTTTAAACTGAGTGTTGTTGCCTTCTCCGTCTTATCTGTTTGCTTTTGGAGATTCCCATGTGTTCCCATTCAGTATCATCTAAATACTTACTTTCAGTTTTATTGCCCAACTATTTCTTTTGCGTGAAGAATTGATTTTCAGTTTGGGTTTTGGCACATCCATGCGAAGGCAGTCTCGCACTTGTGTTTTCAAAATTTAACAAGGGATCTTGTCTATGGTGTCAATTAAATTTTTTTTTCCGCATAACCAAAATTTCAAAGTCTTGAGAAAAGAATTTCTAGAATGGGTTGTTTATTTGTTGCCCCAGTAAATGTTGTATAACTAGACCCCGTTTGATTCAATCATACTTCTGTTGACTTATGTTTGTTACTTTTACTATATCCAGGGTTCTCCCGATCTTGGTGCTGCTAGAGAGGTAGCCGACTACCTTGGAACTCGTCATCATGAGTTTCACTTTACGGTGCAGGTAACTACTTTTTTTTGGGATCAGTTGAAAATCCTTTTTTCAATGTTCACCACCAAAATAATGTGTTTGTGCTTTGTTCCCTTCCATATCAGGAAGGCATAGATGCGCTTGAGGAAGTGATTTACCACATTGAGACATACGATGTGACCACTATCAGAGCTAGCACACCCATGTTCCTTATGTCCCGGAAAATCAAATCTTTGGGGGTGAAAATGGTTCTTTCTGGAGAAGGTTCTGATGAAATTTTTGGAGGTTATTTGTACTTCCACAAGGCACCAAACAAAAAAGAGTTTCATGAAGAAACATGCAGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGCTGTATTTTTTTTTTACTTGTTCTAATTTATTTTCTTTTGATGATATCAGATTAAAGCTCTTCACTTATATGACTGTTTGAGGGCCAATAAATCAACCTCAGCATGGGGTGTTGAGGCCCGTGTCCCTTTTCTTGATAAAGAATTTATAAGCACAGCCATGAGCATGGATCCTGATTGGAAAATGGTATACTTTTTTTTTTAGTTTTCATTCTTGATGAAAAATTTTGGTAGCTTGAGCATGTTGAATTGGCCCCACTATTTACTTTTTTTGAGTTGAACGTGTCTCATCAGTGGACAATTTACTATTTATTGCATAGAAAAAATAGAAAATGTAAGTGATCAATAGGTTAGGTCCTATTATTTTCTGGAAAAGTAGTGAACATTCAAAAGAGAAATATTCTGGTAATAAGATAAATATAATATTTACCTTTGCATGTTTTCTTTTTTTGGGTATTCAATTCTTTAGCTCATGTCACTTAACTGTTGTTATCTAATTGTGGGAATTGTCTTCGCTTAAGTTTGAGCTCTTAAGTGATTGAATTAATTTTGTACTATCTGATTACATCTCTATCATAAAGCTCTCTTGTACAAGTACTATTGGAGCTGTTATTATCAATGAAGACAATTTGTGACGTTAATTACTTGCCATACATTTTTTACTGCAGATCAGGCGTGATCTTGGGAGAATAGAGAAGTGGGTCTTGCGCAATGCATTTGATGACGAGAAGGAACCATATTTGCCCAAGGTAGCTTCTGTCTTTGCAATGTGTCCACTGAATCAGACACGGACCCCATGCTTAGCATGTTTCAGTTGTTTCTTTCGTCTTTTTTAACCAAGGCATTGAATCGTTATGTGTTCTTTATCCAAGGAAGAATATTGAATTTGCCCCACCCCTCCCTGCTTTATAGCACATATTGTACAGGCAAAAGGAACAATTCAGTGATGGTGTTGGGTACAGTTGGATTGATGGTCTGAAGGACCACGCGGAGCAACAAGTAAGCTTTATCTCAAAACTGTCTCACTATTTTTGCTGTATTTAACATATTCAGATTGTCTTTGCTTAAGGAGTAAGGTGAAATAATGCCGCTTTGCCCTCATCAGGTGACTGATGCAATGTTGATGAATGCGAACTTCGTCTACCCAGAAAACACTCCTACTACAAAAGAAGCATATTACTACCGGACAATATTTGAGAAATTCTTCCCTAAGGTGCTGTTTTCTTTTGTTTTTATGACACGAATGGAGTGTGTATTTCCCCAGTCTTCTGATCACGGCTTCCTCATTCTATTTAAGCAAAATACTGATGTATTAGTTTCTCAATTTACAACAGAATGCTGCTAGATCGACTGTCCCAGGAGGTCCAAGTGTGGCATGCAGTACTGCAAAAGCAGTGGAATGGGATGCTGCATGGTCGAAAAATCTCGATCCCTCTGGTCGTGCTGCCCTCGGTGTTCATGTAGCTGCATATGAGGAAAAGCTGAATGCTAAAATTGCTACTGCACTGAATGACTCTCCCCAAGAAATTCAAGTAACCCTCGAGAAAGCTGCAGCCGTAGTA

mRNA sequence

ATGTGTGGAATCCTCGCAGTTTTAGGCTGCGCCGACGGCTCTCAGGCTAAGCGTTCCCGAATCATCGAATTGTCTCGGAGGTTGCGCCATAGAGGTCCCGATTGGAGTGGTTTGCATTGTTTTGAGAACTGTTATCTTGCTCATCAAAGATTGGCTATTGTAGACCCCACTTCCGGGGATCAACCCCTTTACAACGAAGACAAGACCGTCGTTGTTACAGTTAACGGGGAGATCTACAACCACAAGGAATTGAGAGAAAAGCTCAAGGGTCATCAGTTCCGAACTGGCAGTGACTGTGAAGTGATTGCACACCTTTACGAAGAATATGGAGAAGAGTTTGTGGACATGTTGGATGGCATGTTTTCCTTCGTCCTTCTTGATACTCGTAATAAAACTTTTATTGCAGCCAGGGATGCTATTGGGATCACCCCACTATATATGGGATGGGGTCTTGATGGATCAATCTGGTTTGCTTCTGAAATGAAAGCTCTAAGTGATGATTGCGAGAGATTCATTTCTTTTCTTCCTGGGCATATATATTCTAGCAAAGAAGGAGGGTTGAGAAGATGGTATAACCCTCCGTGGTACACAGAGATGGTACCATCAGGTCCATATGATCCTTTGGTTCTACGTAAGGCATTTGAGAAGGCATGTTCTGGACTTACACTTATTAGTTTAACATACTATGCTGTTACTAAGAGACTTATGACAGATGTGCCCTTTGGGGTCCTTTTATCTGGAGGATTGGATTCATCACTAGTAGCTGCTGTTGCCTGCCGCCATTTGGCTGATTCTGAAGCAGCTCGTCAGTGGGGGTCACAATTACACACCTTTTGCGTTGGCTTGAAGGGTTCTCCCGATCTTGGTGCTGCTAGAGAGGTAGCCGACTACCTTGGAACTCGTCATCATGAGTTTCACTTTACGGTGCAGGAAGGCATAGATGCGCTTGAGGAAGTGATTTACCACATTGAGACATACGATGTGACCACTATCAGAGCTAGCACACCCATGTTCCTTATGTCCCGGAAAATCAAATCTTTGGGGGTGAAAATGGTTCTTTCTGGAGAAGGTTCTGATGAAATTTTTGGAGGTTATTTGTACTTCCACAAGGCACCAAACAAAAAAATTAAAGCTCTTCACTTATATGACTGTTTGAGGGCCAATAAATCAACCTCAGCATGGGGTGTTGAGGCCCGTGTCCCTTTTCTTGATAAAGAATTTATAAGCACAGCCATGAGCATGGATCCTGATTGGAAAATGATCAGGCGTGATCTTGGGAGAATAGAGAAGTGGGTCTTGCGCAATGCATTTGATGACGAGAAGGAACCATATTTGCCCAAGCACATATTGTACAGGCAAAAGGAACAATTCAGTGATGGTGTTGGGTACAGTTGGATTGATGGTCTGAAGGACCACGCGGAGCAACAAGTGACTGATGCAATGTTGATGAATGCGAACTTCGTCTACCCAGAAAACACTCCTACTACAAAAGAAGCATATTACTACCGGACAATATTTGAGAAATTCTTCCCTAAGAATGCTGCTAGATCGACTGTCCCAGGAGGTCCAAGTGTGGCATGCAGTACTGCAAAAGCAGTGGAATGGGATGCTGCATGGTCGAAAAATCTCGATCCCTCTGGTCGTGCTGCCCTCGGTGTTCATGTAGCTGCATATGAGGAAAAGCTGAATGCTAAAATTGCTACTGCACTGAATGACTCTCCCCAAGAAATTCAAGTAACCCTCGAGAAAGCTGCAGCCGTAGTA

Coding sequence (CDS)

ATGTGTGGAATCCTCGCAGTTTTAGGCTGCGCCGACGGCTCTCAGGCTAAGCGTTCCCGAATCATCGAATTGTCTCGGAGGTTGCGCCATAGAGGTCCCGATTGGAGTGGTTTGCATTGTTTTGAGAACTGTTATCTTGCTCATCAAAGATTGGCTATTGTAGACCCCACTTCCGGGGATCAACCCCTTTACAACGAAGACAAGACCGTCGTTGTTACAGTTAACGGGGAGATCTACAACCACAAGGAATTGAGAGAAAAGCTCAAGGGTCATCAGTTCCGAACTGGCAGTGACTGTGAAGTGATTGCACACCTTTACGAAGAATATGGAGAAGAGTTTGTGGACATGTTGGATGGCATGTTTTCCTTCGTCCTTCTTGATACTCGTAATAAAACTTTTATTGCAGCCAGGGATGCTATTGGGATCACCCCACTATATATGGGATGGGGTCTTGATGGATCAATCTGGTTTGCTTCTGAAATGAAAGCTCTAAGTGATGATTGCGAGAGATTCATTTCTTTTCTTCCTGGGCATATATATTCTAGCAAAGAAGGAGGGTTGAGAAGATGGTATAACCCTCCGTGGTACACAGAGATGGTACCATCAGGTCCATATGATCCTTTGGTTCTACGTAAGGCATTTGAGAAGGCATGTTCTGGACTTACACTTATTAGTTTAACATACTATGCTGTTACTAAGAGACTTATGACAGATGTGCCCTTTGGGGTCCTTTTATCTGGAGGATTGGATTCATCACTAGTAGCTGCTGTTGCCTGCCGCCATTTGGCTGATTCTGAAGCAGCTCGTCAGTGGGGGTCACAATTACACACCTTTTGCGTTGGCTTGAAGGGTTCTCCCGATCTTGGTGCTGCTAGAGAGGTAGCCGACTACCTTGGAACTCGTCATCATGAGTTTCACTTTACGGTGCAGGAAGGCATAGATGCGCTTGAGGAAGTGATTTACCACATTGAGACATACGATGTGACCACTATCAGAGCTAGCACACCCATGTTCCTTATGTCCCGGAAAATCAAATCTTTGGGGGTGAAAATGGTTCTTTCTGGAGAAGGTTCTGATGAAATTTTTGGAGGTTATTTGTACTTCCACAAGGCACCAAACAAAAAAATTAAAGCTCTTCACTTATATGACTGTTTGAGGGCCAATAAATCAACCTCAGCATGGGGTGTTGAGGCCCGTGTCCCTTTTCTTGATAAAGAATTTATAAGCACAGCCATGAGCATGGATCCTGATTGGAAAATGATCAGGCGTGATCTTGGGAGAATAGAGAAGTGGGTCTTGCGCAATGCATTTGATGACGAGAAGGAACCATATTTGCCCAAGCACATATTGTACAGGCAAAAGGAACAATTCAGTGATGGTGTTGGGTACAGTTGGATTGATGGTCTGAAGGACCACGCGGAGCAACAAGTGACTGATGCAATGTTGATGAATGCGAACTTCGTCTACCCAGAAAACACTCCTACTACAAAAGAAGCATATTACTACCGGACAATATTTGAGAAATTCTTCCCTAAGAATGCTGCTAGATCGACTGTCCCAGGAGGTCCAAGTGTGGCATGCAGTACTGCAAAAGCAGTGGAATGGGATGCTGCATGGTCGAAAAATCTCGATCCCTCTGGTCGTGCTGCCCTCGGTGTTCATGTAGCTGCATATGAGGAAAAGCTGAATGCTAAAATTGCTACTGCACTGAATGACTCTCCCCAAGAAATTCAAGTAACCCTCGAGAAAGCTGCAGCCGTAGTA

Protein sequence

MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGDQPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGMFSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIYSSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVPFGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTAMSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV
Homology
BLAST of MS011629 vs. NCBI nr
Match: XP_022132869.1 (asparagine synthetase [glutamine-hydrolyzing] 2 [Momordica charantia])

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 574/597 (96.15%), Postives = 574/597 (96.15%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY
Sbjct: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSKEGGLRRWYNPPWYTEMVPSGPYD LVLRKAFEK             AVTKRLMTDVP
Sbjct: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDLLVLRKAFEK-------------AVTKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA
Sbjct: 361 IFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK
Sbjct: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 589
           AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV
Sbjct: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 584

BLAST of MS011629 vs. NCBI nr
Match: KAA0036302.1 (asparagine synthetase [Cucumis melo var. makuwa])

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 555/602 (92.19%), Postives = 575/602 (95.51%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERF+SFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYY-----AVTKRL 240
           SSK+GGLRRWYNPPW+TEMVPSG YDPLVLR AFEKA SGL +  L++Y     AVTKRL
Sbjct: 181 SSKQGGLRRWYNPPWFTEMVPSGSYDPLVLRNAFEKASSGLLIFDLSHYCLLQGAVTKRL 240

Query: 241 MTDVPFGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVA 300
           MTDVPFGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVA
Sbjct: 241 MTDVPFGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVA 300

Query: 301 DYLGTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSG 360
           DYLGTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSG
Sbjct: 301 DYLGTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSG 360

Query: 361 EGSDEIFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKE 420
           EGSDEIFGGYLYFHKAPNK         KIKALHLYDCLRANKSTSAWG+EARVPFLDKE
Sbjct: 361 EGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKE 420

Query: 421 FISTAMSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWI 480
           FI+ AMS+DPDWKMIRRD+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWI
Sbjct: 421 FINIAMSIDPDWKMIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWI 480

Query: 481 DGLKDHAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVA 540
           DGLKDHAE+QVTD+ML+NANF+YPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVA
Sbjct: 481 DGLKDHAEKQVTDSMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVA 540

Query: 541 CSTAKAVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAA 589
           CSTAKAVEWDAAWSKNLDPSGRAALGVHVAAY+EKLN KIA   NDSPQEI+VT+E+AAA
Sbjct: 541 CSTAKAVEWDAAWSKNLDPSGRAALGVHVAAYDEKLNGKIANVPNDSPQEIKVTVEQAAA 600

BLAST of MS011629 vs. NCBI nr
Match: XP_008440595.1 (PREDICTED: asparagine synthetase [glutamine-hydrolyzing] 2 [Cucumis melo] >TYK12696.1 asparagine synthetase [Cucumis melo var. makuwa])

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 549/597 (91.96%), Postives = 566/597 (94.81%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERF+SFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK+GGLRRWYNPPW+TEMVPSG YDPLVLR AFEK             AVTKRLMTDVP
Sbjct: 181 SSKQGGLRRWYNPPWFTEMVPSGSYDPLVLRNAFEK-------------AVTKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALHLYDCLRANKSTSAWG+EARVPFLDKEFI+ A
Sbjct: 361 IFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFINIA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DPDWKMIRRD+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSIDPDWKMIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HAE+QVTD+ML+NANF+YPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK
Sbjct: 481 HAEKQVTDSMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 589
           AVEWDAAWSKNLDPSGRAALGVHVAAY+EKLN KIA   NDSPQEI+VT+E+AAAVV
Sbjct: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYDEKLNGKIANVPNDSPQEIKVTVEQAAAVV 584

BLAST of MS011629 vs. NCBI nr
Match: XP_031743076.1 (asparagine synthetase [glutamine-hydrolyzing] 2 [Cucumis sativus] >KGN48750.1 hypothetical protein Csa_003204 [Cucumis sativus])

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 549/597 (91.96%), Postives = 565/597 (94.64%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERF+SFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK+G LRRWYNPPW+TEMVPSG YDPLVLR AFEK             AVTKRLMTDVP
Sbjct: 181 SSKQGELRRWYNPPWFTEMVPSGSYDPLVLRNAFEK-------------AVTKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDL AAREVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLSAAREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALHLYDCLRANKSTSAWG+EARVPFLDKEFI+ A
Sbjct: 361 IFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFINIA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DP+WKMIRRD+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSIDPEWKMIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HAE+QVTDAML+NANF+YPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK
Sbjct: 481 HAEKQVTDAMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 589
           AVEWDAAWSKNLDPSGRAALGVHVAAY+EKLNAKIA   NDSPQEI+VT+EKAAAVV
Sbjct: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYDEKLNAKIANVPNDSPQEIKVTVEKAAAVV 584

BLAST of MS011629 vs. NCBI nr
Match: XP_038882037.1 (asparagine synthetase [glutamine-hydrolyzing] 2 [Benincasa hispida])

HSP 1 Score: 1129.0 bits (2919), Expect = 0.0e+00
Identity = 551/597 (92.29%), Postives = 564/597 (94.47%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTVVVTVNGEIYNHKELREKLKGH+FRTGSDCEVIAHLYEEYGE+FVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHEFRTGSDCEVIAHLYEEYGEDFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERF+SFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK+GGLRRWYNPPWYTEMVPSG YDPLVLRKAFEK             AVTKRLMTDVP
Sbjct: 181 SSKQGGLRRWYNPPWYTEMVPSGHYDPLVLRKAFEK-------------AVTKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVAAVA RHL DSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVAAVASRHLVDSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALHLYDCLRANKSTSAWG+EARVPFLDKEFIS A
Sbjct: 361 IFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DP+WKMIR DLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSIDPEWKMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HAE+QVTDAMLMNA+F+YPENTP TKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK
Sbjct: 481 HAEKQVTDAMLMNASFIYPENTPMTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 589
           AVEWDAAWSKNLDPSGRAALGVHVAAY+EKLNAKIA   NDSPQEIQVT+EKAAAVV
Sbjct: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYDEKLNAKIANVPNDSPQEIQVTVEKAAAVV 584

BLAST of MS011629 vs. ExPASy Swiss-Prot
Match: Q9LV77 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Arabidopsis thaliana OX=3702 GN=ASN2 PE=2 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 1.8e-296
Identity = 489/581 (84.17%), Postives = 524/581 (90.19%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHC+E+CYLAH+RLAI+DPTSGD
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTV VTVNGEIYNHK LREKLK HQFRTGSDCEVIAHLYEE+GEEF+DMLDGM
Sbjct: 61  QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           F+FVLLDTR+K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+SF PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK+GGLRRWYNPPWY E VPS PYDPLVLR AFEK             AV KRLMTDVP
Sbjct: 181 SSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEK-------------AVIKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVAAVA RHL  SEAARQWGSQLHTFC+GL+GSPDL A REVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHEF FTVQ+GIDA+EEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHEFQFTVQDGIDAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           I GGYLYFHKAPNK         KIKALH +DCLRANKSTSAWGVEARVPFLDKEF++ A
Sbjct: 361 ILGGYLYFHKAPNKKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DP+WK+I+ DLGRIEKWVLRNAFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSIDPEWKLIKPDLGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HA + V+D ML NA+FV+P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPS+ACSTAK
Sbjct: 481 HANKHVSDTMLSNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSIACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALND 573
           AVEWDA WSKNLDPSGRAALGVHVAAYEE   A  A A +D
Sbjct: 541 AVEWDATWSKNLDPSGRAALGVHVAAYEEDKAAAAAKAGSD 568

BLAST of MS011629 vs. ExPASy Swiss-Prot
Match: Q9LFU1 (Asparagine synthetase [glutamine-hydrolyzing] 3 OS=Arabidopsis thaliana OX=3702 GN=ASN3 PE=2 SV=1)

HSP 1 Score: 1016.1 bits (2626), Expect = 1.5e-295
Identity = 489/597 (81.91%), Postives = 530/597 (88.78%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHC+E+CYLAH+RLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKT+ VTVNGEIYNHK LRE LK HQFRTGSDCEVIAHLYEE+GEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLYEEHGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           F+FVLLDTR+K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+ F PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK+GGLRRWYNPPW++E+VPS PYDPLV+R  FEK             AV KRLMTDVP
Sbjct: 181 SSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEK-------------AVIKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVA+VA RHL  SEAA QWGS+LHTFC+GLKGSPDL A REVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHE HFTVQ+GIDA+EEVIYH+ETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHELHFTVQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALH YDCLRANKSTSAWGVEARVPFLDKEFI+ A
Sbjct: 361 IFGGYLYFHKAPNKKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DP+WKMIR DLGRIEKWVLRNAFDDEK PYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSIDPEWKMIRPDLGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HA + V++ MLMNA+FV+P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPSVACSTAK
Sbjct: 481 HANKHVSETMLMNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 589
           AVEWDAAWS+NLDPSGRAALGVHV+AY E          +  P+++Q   EK  A+V
Sbjct: 541 AVEWDAAWSQNLDPSGRAALGVHVSAYGEDKTE------DSRPEKLQKLAEKTPAIV 578

BLAST of MS011629 vs. ExPASy Swiss-Prot
Match: Q43011 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0265000 PE=2 SV=3)

HSP 1 Score: 1003.0 bits (2592), Expect = 1.4e-291
Identity = 478/578 (82.70%), Postives = 523/578 (90.48%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLG AD S AKRSRIIELSRRLRHRGPDWSG+HC+++CYLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGVADVSLAKRSRIIELSRRLRHRGPDWSGIHCYQDCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDK+VVVTVNGEIYNH+EL+  LK H+F+T SDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKSVVVTVNGEIYNHEELKANLKSHKFQTASDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           F+FVLLDTR+K+FIAARDAIGI PLYMGWGLDGS+WF+SEMKALSDDCERFISF PGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGICPLYMGWGLDGSVWFSSEMKALSDDCERFISFPPGHLY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK GGLRRWYNPPW++E +PS PY+PL+LR++FEK             A+ KRLMTDVP
Sbjct: 181 SSKTGGLRRWYNPPWFSESIPSTPYNPLLLRQSFEK-------------AIIKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVA+V  RHLA+++ A QWG++LHTFC+GLKGSPDL AA+EVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVASVVSRHLAEAKVAAQWGNKLHTFCIGLKGSPDLRAAKEVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
            HHE HFTVQEGIDALEEVIYH+ETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 VHHELHFTVQEGIDALEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALHLYDCLRANKSTSAWGVEARVPFLDK FI+ A
Sbjct: 361 IFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKNFINVA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           M +DP+WKMI+RDLGRIEKWVLRNAFDDE++PYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MDIDPEWKMIKRDLGRIEKWVLRNAFDDEEKPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HA + V+D+M+MNA+FVYPENTP TKEAYYYRTIFEKFFPKNAAR TVPGGPSVACSTAK
Sbjct: 481 HANEHVSDSMMMNASFVYPENTPVTKEAYYYRTIFEKFFPKNAARLTVPGGPSVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATA 570
           AVEWDAAWSKNLDPSGRAALGVH AAYE+ L    A+A
Sbjct: 541 AVEWDAAWSKNLDPSGRAALGVHDAAYEDTLQKSPASA 565

BLAST of MS011629 vs. ExPASy Swiss-Prot
Match: P31752 (Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 PE=2 SV=2)

HSP 1 Score: 931.0 bits (2405), Expect = 6.5e-270
Identity = 440/576 (76.39%), Postives = 498/576 (86.46%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC+D SQAKR R++ELSRRL+HRGPDWSGL    +C+L+HQRLAI+DP SGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCFLSHQRLAIIDPASGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDK++VVTVNGEIYNH+ELR +L  H++RTGSDCEVIAHLYEE+GE+FVDMLDGM
Sbjct: 61  QPLYNEDKSIVVTVNGEIYNHEELRRRLPDHKYRTGSDCEVIAHLYEEHGEDFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTRN  F+AARDA+GITPLY+GWGLDGS+W +SEMK L+DDCE F  F PG++Y
Sbjct: 121 FSFVLLDTRNNCFVAARDAVGITPLYIGWGLDGSVWLSSEMKGLNDDCEHFEVFPPGNLY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SS+ G  RRWYNP WY E +PS PYDPLVLRKAFE              AV KRLMTDVP
Sbjct: 181 SSRSGSFRRWYNPQWYNETIPSAPYDPLVLRKAFED-------------AVIKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVAAV  RHLA S+AA QWG+QLH+FCVGL+GSPDL AA+EVA+YLGT
Sbjct: 241 FGVLLSGGLDSSLVAAVTARHLAGSKAAEQWGTQLHSFCVGLEGSPDLKAAKEVAEYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
            HHEFHFTVQ+GIDA+E+VI+HIETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEGSDE
Sbjct: 301 VHHEFHFTVQDGIDAIEDVIFHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALH YDCLRANK+TSAWG+EARVPFLDKEF+  A
Sbjct: 361 IFGGYLYFHKAPNKEEFHHETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFMDVA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DP+ KMI+ DLGRIEKWVLR AFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLK 
Sbjct: 421 MSIDPESKMIKPDLGRIEKWVLRKAFDDEENPYLPKHILYRQKEQFSDGVGYSWIDGLKA 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HA + VTD M++NA  +YP NTPTTKEAYYYR IFE+FFP+N+AR TVPGGPS+ACSTAK
Sbjct: 481 HAAKHVTDRMMLNAARIYPHNTPTTKEAYYYRMIFERFFPQNSARFTVPGGPSIACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIA 568
           A+EWDA WS NLDPSGRAALGVH +AY+  L + I+
Sbjct: 541 AIEWDARWSNNLDPSGRAALGVHDSAYDPPLPSSIS 563

BLAST of MS011629 vs. ExPASy Swiss-Prot
Match: P49094 (Asparagine synthetase [glutamine-hydrolyzing] OS=Zea mays OX=4577 GN=ASN1 PE=2 SV=2)

HSP 1 Score: 923.3 bits (2385), Expect = 1.4e-267
Identity = 441/557 (79.17%), Postives = 484/557 (86.89%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLG  + S AKRSRIIELSRRLRHRGPDWSGLHC E+CYLAHQRLAI+DPTSGD
Sbjct: 1   MCGILAVLGVVEVSLAKRSRIIELSRRLRHRGPDWSGLHCHEDCYLAHQRLAIIDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTVVVTVNGEIYNH+EL+ KLK H+F+TGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHEELKAKLKTHEFQTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTR+K+FIAARDAIGI PLYMGWGLDGS+WF+SEMKALSDDCERFI+F PGH+Y
Sbjct: 121 FSFVLLDTRDKSFIAARDAIGICPLYMGWGLDGSVWFSSEMKALSDDCERFITFPPGHLY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK GGLRRWYNPPW++E VPS PY+ L LR+ FEK             AV KRLMTDVP
Sbjct: 181 SSKTGGLRRWYNPPWFSETVPSTPYNALFLREMFEK-------------AVIKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVA+VA RHL +++  RQWG++LHTFC+GLKGSPDL AAREVADYL T
Sbjct: 241 FGVLLSGGLDSSLVASVASRHLNETKVDRQWGNKLHTFCIGLKGSPDLKAAREVADYLST 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
            HHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMV+SGEGSDE
Sbjct: 301 VHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALHLYDCLRANK+TSAWGVEARVPFLDK FIS A
Sbjct: 361 IFGGYLYFHKAPNKKEFLEETCRKIKALHLYDCLRANKATSAWGVEARVPFLDKSFISVA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           M +DP+W MI+RDLGRIEKWV+R AFDD++ PYLPKHILYRQKEQFSDGVGY+WIDGLK 
Sbjct: 421 MDIDPEWNMIKRDLGRIEKWVMRKAFDDDEHPYLPKHILYRQKEQFSDGVGYNWIDGLKS 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
             EQQVTD M+ NA  ++P NTP  KEAYYYR IFE+ FP+++AR TVP GPS+ACST  
Sbjct: 481 FTEQQVTDEMMNNAAQMFPYNTPVNKEAYYYRMIFERLFPQDSARETVPWGPSIACSTPA 540

Query: 541 AVEWDAAWSKNLDPSGR 549
           A+EW   W  + DPSGR
Sbjct: 541 AIEWVEQWKASNDPSGR 544

BLAST of MS011629 vs. ExPASy TrEMBL
Match: A0A6J1BUB1 (Asparagine synthetase [glutamine-hydrolyzing] OS=Momordica charantia OX=3673 GN=LOC111005610 PE=4 SV=1)

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 574/597 (96.15%), Postives = 574/597 (96.15%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY
Sbjct: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSKEGGLRRWYNPPWYTEMVPSGPYD LVLRKAFEK             AVTKRLMTDVP
Sbjct: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDLLVLRKAFEK-------------AVTKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA
Sbjct: 361 IFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK
Sbjct: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 589
           AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV
Sbjct: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 584

BLAST of MS011629 vs. ExPASy TrEMBL
Match: A0A5A7SYK6 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G001010 PE=4 SV=1)

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 555/602 (92.19%), Postives = 575/602 (95.51%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERF+SFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYY-----AVTKRL 240
           SSK+GGLRRWYNPPW+TEMVPSG YDPLVLR AFEKA SGL +  L++Y     AVTKRL
Sbjct: 181 SSKQGGLRRWYNPPWFTEMVPSGSYDPLVLRNAFEKASSGLLIFDLSHYCLLQGAVTKRL 240

Query: 241 MTDVPFGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVA 300
           MTDVPFGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVA
Sbjct: 241 MTDVPFGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVA 300

Query: 301 DYLGTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSG 360
           DYLGTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSG
Sbjct: 301 DYLGTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSG 360

Query: 361 EGSDEIFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKE 420
           EGSDEIFGGYLYFHKAPNK         KIKALHLYDCLRANKSTSAWG+EARVPFLDKE
Sbjct: 361 EGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKE 420

Query: 421 FISTAMSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWI 480
           FI+ AMS+DPDWKMIRRD+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWI
Sbjct: 421 FINIAMSIDPDWKMIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWI 480

Query: 481 DGLKDHAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVA 540
           DGLKDHAE+QVTD+ML+NANF+YPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVA
Sbjct: 481 DGLKDHAEKQVTDSMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVA 540

Query: 541 CSTAKAVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAA 589
           CSTAKAVEWDAAWSKNLDPSGRAALGVHVAAY+EKLN KIA   NDSPQEI+VT+E+AAA
Sbjct: 541 CSTAKAVEWDAAWSKNLDPSGRAALGVHVAAYDEKLNGKIANVPNDSPQEIKVTVEQAAA 600

BLAST of MS011629 vs. ExPASy TrEMBL
Match: A0A5D3CQI6 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002840 PE=4 SV=1)

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 549/597 (91.96%), Postives = 566/597 (94.81%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERF+SFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK+GGLRRWYNPPW+TEMVPSG YDPLVLR AFEK             AVTKRLMTDVP
Sbjct: 181 SSKQGGLRRWYNPPWFTEMVPSGSYDPLVLRNAFEK-------------AVTKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALHLYDCLRANKSTSAWG+EARVPFLDKEFI+ A
Sbjct: 361 IFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFINIA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DPDWKMIRRD+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSIDPDWKMIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HAE+QVTD+ML+NANF+YPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK
Sbjct: 481 HAEKQVTDSMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 589
           AVEWDAAWSKNLDPSGRAALGVHVAAY+EKLN KIA   NDSPQEI+VT+E+AAAVV
Sbjct: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYDEKLNGKIANVPNDSPQEIKVTVEQAAAVV 584

BLAST of MS011629 vs. ExPASy TrEMBL
Match: A0A1S3B235 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC103484971 PE=4 SV=1)

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 549/597 (91.96%), Postives = 566/597 (94.81%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERF+SFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK+GGLRRWYNPPW+TEMVPSG YDPLVLR AFEK             AVTKRLMTDVP
Sbjct: 181 SSKQGGLRRWYNPPWFTEMVPSGSYDPLVLRNAFEK-------------AVTKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALHLYDCLRANKSTSAWG+EARVPFLDKEFI+ A
Sbjct: 361 IFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFINIA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DPDWKMIRRD+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSIDPDWKMIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HAE+QVTD+ML+NANF+YPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK
Sbjct: 481 HAEKQVTDSMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 589
           AVEWDAAWSKNLDPSGRAALGVHVAAY+EKLN KIA   NDSPQEI+VT+E+AAAVV
Sbjct: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYDEKLNGKIANVPNDSPQEIKVTVEQAAAVV 584

BLAST of MS011629 vs. ExPASy TrEMBL
Match: A0A0A0KG93 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis sativus OX=3659 GN=Csa_6G500420 PE=4 SV=1)

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 549/597 (91.96%), Postives = 565/597 (94.64%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERF+SFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK+G LRRWYNPPW+TEMVPSG YDPLVLR AFEK             AVTKRLMTDVP
Sbjct: 181 SSKQGELRRWYNPPWFTEMVPSGSYDPLVLRNAFEK-------------AVTKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDL AAREVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLSAAREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALHLYDCLRANKSTSAWG+EARVPFLDKEFI+ A
Sbjct: 361 IFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFINIA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DP+WKMIRRD+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSIDPEWKMIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HAE+QVTDAML+NANF+YPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK
Sbjct: 481 HAEKQVTDAMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 589
           AVEWDAAWSKNLDPSGRAALGVHVAAY+EKLNAKIA   NDSPQEI+VT+EKAAAVV
Sbjct: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYDEKLNAKIANVPNDSPQEIKVTVEKAAAVV 584

BLAST of MS011629 vs. TAIR 10
Match: AT5G65010.1 (asparagine synthetase 2 )

HSP 1 Score: 1019.2 bits (2634), Expect = 1.3e-297
Identity = 489/581 (84.17%), Postives = 524/581 (90.19%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHC+E+CYLAH+RLAI+DPTSGD
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTV VTVNGEIYNHK LREKLK HQFRTGSDCEVIAHLYEE+GEEF+DMLDGM
Sbjct: 61  QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           F+FVLLDTR+K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+SF PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK+GGLRRWYNPPWY E VPS PYDPLVLR AFEK             AV KRLMTDVP
Sbjct: 181 SSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEK-------------AVIKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVAAVA RHL  SEAARQWGSQLHTFC+GL+GSPDL A REVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHEF FTVQ+GIDA+EEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHEFQFTVQDGIDAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           I GGYLYFHKAPNK         KIKALH +DCLRANKSTSAWGVEARVPFLDKEF++ A
Sbjct: 361 ILGGYLYFHKAPNKKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DP+WK+I+ DLGRIEKWVLRNAFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSIDPEWKLIKPDLGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HA + V+D ML NA+FV+P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPS+ACSTAK
Sbjct: 481 HANKHVSDTMLSNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSIACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALND 573
           AVEWDA WSKNLDPSGRAALGVHVAAYEE   A  A A +D
Sbjct: 541 AVEWDATWSKNLDPSGRAALGVHVAAYEEDKAAAAAKAGSD 568

BLAST of MS011629 vs. TAIR 10
Match: AT5G10240.1 (asparagine synthetase 3 )

HSP 1 Score: 1016.1 bits (2626), Expect = 1.1e-296
Identity = 489/597 (81.91%), Postives = 530/597 (88.78%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHC+E+CYLAH+RLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKT+ VTVNGEIYNHK LRE LK HQFRTGSDCEVIAHLYEE+GEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLYEEHGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           F+FVLLDTR+K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+ F PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK+GGLRRWYNPPW++E+VPS PYDPLV+R  FEK             AV KRLMTDVP
Sbjct: 181 SSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEK-------------AVIKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVA+VA RHL  SEAA QWGS+LHTFC+GLKGSPDL A REVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHE HFTVQ+GIDA+EEVIYH+ETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHELHFTVQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALH YDCLRANKSTSAWGVEARVPFLDKEFI+ A
Sbjct: 361 IFGGYLYFHKAPNKKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DP+WKMIR DLGRIEKWVLRNAFDDEK PYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSIDPEWKMIRPDLGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HA + V++ MLMNA+FV+P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPSVACSTAK
Sbjct: 481 HANKHVSETMLMNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 589
           AVEWDAAWS+NLDPSGRAALGVHV+AY E          +  P+++Q   EK  A+V
Sbjct: 541 AVEWDAAWSQNLDPSGRAALGVHVSAYGEDKTE------DSRPEKLQKLAEKTPAIV 578

BLAST of MS011629 vs. TAIR 10
Match: AT5G65010.2 (asparagine synthetase 2 )

HSP 1 Score: 1014.6 bits (2622), Expect = 3.2e-296
Identity = 489/582 (84.02%), Postives = 524/582 (90.03%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHC+E+CYLAH+RLAI+DPTSGD
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKTV VTVNGEIYNHK LREKLK HQFRTGSDCEVIAHLYEE+GEEF+DMLDGM
Sbjct: 61  QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           F+FVLLDTR+K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+SF PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK+GGLRRWYNPPWY E VPS PYDPLVLR AFEK             AV KRLMTDVP
Sbjct: 181 SSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEK-------------AVIKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVAAVA RHL  SEAARQWGSQLHTFC+GL+GSPDL A REVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHEF FTVQ+GIDA+EEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHEFQFTVQDGIDAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           I GGYLYFHKAPNK         KIKALH +DCLRANKSTSAWGVEARVPFLDKEF++ A
Sbjct: 361 ILGGYLYFHKAPNKKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DP+WK+I+ DLGRIEKWVLRNAFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSIDPEWKLIKPDLGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPK-NAARSTVPGGPSVACSTA 540
           HA + V+D ML NA+FV+P+NTP TKEAYYYRTIFEKFFPK +AAR+TVPGGPS+ACSTA
Sbjct: 481 HANKHVSDTMLSNASFVFPDNTPLTKEAYYYRTIFEKFFPKQSAARATVPGGPSIACSTA 540

Query: 541 KAVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALND 573
           KAVEWDA WSKNLDPSGRAALGVHVAAYEE   A  A A +D
Sbjct: 541 KAVEWDATWSKNLDPSGRAALGVHVAAYEEDKAAAAAKAGSD 569

BLAST of MS011629 vs. TAIR 10
Match: AT5G10240.2 (asparagine synthetase 3 )

HSP 1 Score: 1005.0 bits (2597), Expect = 2.5e-293
Identity = 486/597 (81.41%), Postives = 528/597 (88.44%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC D SQAKRSRIIELSRRLRHRGPDWSGLHC+E+CYLAH+RLAIVDPTSGD
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPLYNEDKT+ VTVNGEIYNHK LRE LK HQFRTGSDCEVIAHL  ++GEEFVDMLDGM
Sbjct: 61  QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLV-KHGEEFVDMLDGM 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           F+FVLLDTR+K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+ F PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK+GGLRRWYNPPW++E+VPS PYDPLV+R  FEK             AV KRLMTDVP
Sbjct: 181 SSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEK-------------AVIKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVA+VA RHL  SEAA QWGS+LHTFC+GLKGSPDL A REVADYLGT
Sbjct: 241 FGVLLSGGLDSSLVASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
           RHHE HFTVQ+GIDA+EEVIYH+ETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE
Sbjct: 301 RHHELHFTVQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALH YDCLRANKSTSAWGVEARVPFLDKEFI+ A
Sbjct: 361 IFGGYLYFHKAPNKKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DP+WKMIR DLGRIEKWVLRNAFDDEK PYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSIDPEWKMIRPDLGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HA + V++ MLMNA+FV+P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPSVACSTAK
Sbjct: 481 HANKHVSETMLMNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEKLNAKIATALNDSPQEIQVTLEKAAAVV 589
           AVEWDAAWS+NLDPSGRAALGVHV+AY E          +  P+++Q   EK  A+V
Sbjct: 541 AVEWDAAWSQNLDPSGRAALGVHVSAYGEDKTE------DSRPEKLQKLAEKTPAIV 577

BLAST of MS011629 vs. TAIR 10
Match: AT3G47340.1 (glutamine-dependent asparagine synthase 1 )

HSP 1 Score: 913.7 bits (2360), Expect = 7.7e-266
Identity = 428/570 (75.09%), Postives = 495/570 (86.84%), Query Frame = 0

Query: 1   MCGILAVLGCADGSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
           MCGILAVLGC+D SQAKR R++ELSRRLRHRGPDWSGL+   + YLAHQRLA++DP SGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGDNYLAHQRLAVIDPASGD 60

Query: 61  QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
           QPL+NEDKT+VVTVNGEIYNH+ELR++LK H+FRTGSDCEVIAHLYEEYG +FVDMLDG+
Sbjct: 61  QPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVIAHLYEEYGVDFVDMLDGI 120

Query: 121 FSFVLLDTRNKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFISFLPGHIY 180
           FSFVLLDTR+ +F+ ARDAIG+T LY+GWGLDGS+W +SEMK L+DDCE F +F PGH Y
Sbjct: 121 FSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFPPGHFY 180

Query: 181 SSKEGGLRRWYNPPWYTEMVPSGPYDPLVLRKAFEKACSGLTLISLTYYAVTKRLMTDVP 240
           SSK GG ++WYNPPW+ E VPS PY+PL +R+AFE              AV KRLMTDVP
Sbjct: 181 SSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFEN-------------AVIKRLMTDVP 240

Query: 241 FGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGT 300
           FGVLLSGGLDSSLVA++  RHLA ++AA+QWG QLH+FCVGL+GSPDL A +EVA+YLGT
Sbjct: 241 FGVLLSGGLDSSLVASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGT 300

Query: 301 RHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDE 360
            HHEFHF+VQ+GIDA+E+VIYH+ETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEG+DE
Sbjct: 301 VHHEFHFSVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADE 360

Query: 361 IFGGYLYFHKAPNK---------KIKALHLYDCLRANKSTSAWGVEARVPFLDKEFISTA 420
           IFGGYLYFHKAPNK         KIKALH YDCLRANKSTSA+G+EARVPFLDK+FI+TA
Sbjct: 361 IFGGYLYFHKAPNKKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTA 420

Query: 421 MSMDPDWKMIRRDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480
           MS+DP+ KMI+ + GRIEKWVLR AFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLKD
Sbjct: 421 MSLDPESKMIKPEEGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKD 480

Query: 481 HAEQQVTDAMLMNANFVYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAK 540
           HA Q V D M+ NA  ++P NTP TKEAYYYR IFE+FFP+N+AR TVPGG +VACSTAK
Sbjct: 481 HAAQNVNDKMMSNAGHIFPHNTPNTKEAYYYRMIFERFFPQNSARLTVPGGATVACSTAK 540

Query: 541 AVEWDAAWSKNLDPSGRAALGVHVAAYEEK 562
           AVEWDA+WS N+DPSGRAA+GVH++AY+ K
Sbjct: 541 AVEWDASWSNNMDPSGRAAIGVHLSAYDGK 557

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132869.10.0e+0096.15asparagine synthetase [glutamine-hydrolyzing] 2 [Momordica charantia][more]
KAA0036302.10.0e+0092.19asparagine synthetase [Cucumis melo var. makuwa][more]
XP_008440595.10.0e+0091.96PREDICTED: asparagine synthetase [glutamine-hydrolyzing] 2 [Cucumis melo] >TYK12... [more]
XP_031743076.10.0e+0091.96asparagine synthetase [glutamine-hydrolyzing] 2 [Cucumis sativus] >KGN48750.1 hy... [more]
XP_038882037.10.0e+0092.29asparagine synthetase [glutamine-hydrolyzing] 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LV771.8e-29684.17Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LFU11.5e-29581.91Asparagine synthetase [glutamine-hydrolyzing] 3 OS=Arabidopsis thaliana OX=3702 ... [more]
Q430111.4e-29182.70Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica ... [more]
P317526.5e-27076.39Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 P... [more]
P490941.4e-26779.17Asparagine synthetase [glutamine-hydrolyzing] OS=Zea mays OX=4577 GN=ASN1 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1BUB10.0e+0096.15Asparagine synthetase [glutamine-hydrolyzing] OS=Momordica charantia OX=3673 GN=... [more]
A0A5A7SYK60.0e+0092.19Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3CQI60.0e+0091.96Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3B2350.0e+0091.96Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A0A0KG930.0e+0091.96Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis sativus OX=3659 GN=Csa_... [more]
Match NameE-valueIdentityDescription
AT5G65010.11.3e-29784.17asparagine synthetase 2 [more]
AT5G10240.11.1e-29681.91asparagine synthetase 3 [more]
AT5G65010.23.2e-29684.02asparagine synthetase 2 [more]
AT5G10240.22.5e-29381.41asparagine synthetase 3 [more]
AT3G47340.17.7e-26675.09glutamine-dependent asparagine synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006426Asparagine synthase, glutamine-hydrolyzingPIRSFPIRSF001589Asn_synthetase_glu-hcoord: 370..521
e-value: 7.0E-25
score: 85.4
coord: 1..377
e-value: 3.1E-110
score: 367.3
IPR006426Asparagine synthase, glutamine-hydrolyzingTIGRFAMTIGR01536TIGR01536coord: 2..375
e-value: 1.7E-112
score: 375.0
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 218..518
e-value: 9.6E-109
score: 365.8
IPR017932Glutamine amidotransferase type 2 domainPFAMPF13537GATase_7coord: 48..165
e-value: 4.8E-45
score: 152.5
IPR017932Glutamine amidotransferase type 2 domainPROSITEPS51278GATASE_TYPE_2coord: 2..185
score: 38.61161
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 3..192
e-value: 2.2E-63
score: 215.0
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 1..195
IPR001962Asparagine synthasePFAMPF00733Asn_synthasecoord: 375..515
e-value: 1.5E-35
score: 123.5
coord: 227..373
e-value: 5.3E-57
score: 194.1
IPR001962Asparagine synthaseCDDcd01991Asn_Synthase_B_Ccoord: 230..460
e-value: 3.11912E-74
score: 236.162
NoneNo IPR availablePIRSRPIRSR001589-1PIRSR001589-1coord: 1..556
e-value: 4.5E-245
score: 812.1
NoneNo IPR availablePIRSRPIRSR000485-1PIRSR000485-1coord: 1..43
e-value: 1.5
score: 5.1
NoneNo IPR availablePIRSRPIRSR001589-2PIRSR001589-2coord: 1..556
e-value: 4.5E-245
score: 812.1
NoneNo IPR availablePIRSRPIRSR001589-3PIRSR001589-3coord: 1..556
e-value: 4.5E-245
score: 812.1
NoneNo IPR availablePANTHERPTHR11772:SF38ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 3coord: 1..585
NoneNo IPR availablePANTHERPTHR11772ASPARAGINE SYNTHETASEcoord: 1..585
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 223..488
IPR033738Asparagine synthase, N-terminal domainCDDcd00712AsnBcoord: 2..192
e-value: 9.27655E-88
score: 269.427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS011629.1MS011629.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006529 asparagine biosynthetic process
molecular_function GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity