MS011492 (gene) Bitter gourd (TR) v1

Overview
NameMS011492
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionN-acetyltransferase domain-containing protein
Locationscaffold239: 2385782 .. 2387352 (+)
RNA-Seq ExpressionMS011492
SyntenyMS011492
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAATAGTGATTAGAGAGTTCGATGCGAGTAAAGATGGCTTGGGGGTGGAAGATGTGGAGAGAAGGTGCGAAGTTGGACCAAGCGGCAAACTCTGTCTTTTCACCGACCTCTTGGGTGACCCAATTTGCAGGGTCCGCAATTCCCCTGCCTTCCTCATGCTGGTATATATTTTTCTTTTTTTTTTTAATTTTCCATTTCTTTCTAATCCCCTCCCCTTCCCCTTCCCCTTCCCCTTCCCCCATACTTTCACCTATCCAATCCCCTTTTGTCTTTTTCCAGAATTTGTAACCCCCCGAACCAACCCTCTCTCTCTTATAACAATAACAATAAATGCGAGGGAGAGGGAGAGGGTTTGGTTTGGTAATTGGGTTTTGTTGTTTGTGCTGACCCTCATTATGGTGTTGGCGTTTCAACAGGTGGCGTCGACGGGCGACGAGAACGAGATACTGGGGATGATTAGAGGCTGCATCAAAACCGTTACATGCGGTAAAAAACTCTCCCGAACTCCCAAAACCACCACCCACGACGATCCCCCCTCCAAACACGTCCCCGTTTTCACTAAACTCGCCTACATCTTGGGCCTCCGCGTCTCTCCCGCCCACCGGTATGTATCATCAACATATTCATTTCTTTCTCTCTTTTTTTTCCTTGTTTTTTGGGTGATTGTAGAGGTTTTTTTCCTTTCTGATTGGATTGGTGGGCGTATTGTGTTTTCAGGAGGATGGGAATTGGGCTGAAGCTGGTGCGCGGAATGGAGGAGTGGTTCAGAGAGAATGGCGCCGAGTATTCGTACATAGCAACGGAAAACGACAACGTTGCTTCGGTTAATCTGTTCACCGAAAAATGCGGCTACTCCAAGTTCCGTACACCGGCCATCCTTGTCAATCCCGTGTTCGCCCACCGAGTCCCGCTGTCCCATCGCGTCACAATCCTCCCACTCTCACGCGCCGACGCCGAGATTCTCTATCGCCGCCGCTTCTCCACCACCGAGTTTTTCCCCCGCGACATTGACTCCGTCCTCGGTAACAAACTCACACTCGGCACCTTTCTCGCGCTGCCACGTGGCTCTTACTCCTGGACCGGGTCCGACCGCTTCCTGCTCGACCCGCCCGAGTCGTGGGCGGTTCTGAGTGTGTGGAACTGCAAGGACGTGTTCACGCTCCAGGTACGTGGAGCGTCACGCGTGAAGCGGACGTTGGCGAAAACGACACGCGTCTTGGATCGGGCGTTTCCGTGGCTGCGCCTGCCTTCGGTGCCGGAGCTGTTCAGGCCGTTCGGGCTGCACTTTCTGTACGGGCTGGGAGGGGAGGGCCCGGCGGCGGGGAAGTTGATGAAGGCGCTGTGTGGGTACGCGCACAATCTGGCCAAGGAGCGTGGGTGTGGGGTGGTGGCGACGGAGGTGTCGGCGAGGGAGCCGCTCCGGCCGGCGATTCCTCACTGGAAAATGCTGTCGTGCGAGGAGGATCTGTGGTGCATCAAGCGCCTCGGGGAAGACTATAGCGACGGCTCTGTCGGTGACTGGACCAAATCACCACCTGGCTTGTCGATTTTTGTCGACCCCAGAGAGTTC

mRNA sequence

GAAATAGTGATTAGAGAGTTCGATGCGAGTAAAGATGGCTTGGGGGTGGAAGATGTGGAGAGAAGGTGCGAAGTTGGACCAAGCGGCAAACTCTGTCTTTTCACCGACCTCTTGGGTGACCCAATTTGCAGGGTCCGCAATTCCCCTGCCTTCCTCATGCTGGTGGCGTCGACGGGCGACGAGAACGAGATACTGGGGATGATTAGAGGCTGCATCAAAACCGTTACATGCGGTAAAAAACTCTCCCGAACTCCCAAAACCACCACCCACGACGATCCCCCCTCCAAACACGTCCCCGTTTTCACTAAACTCGCCTACATCTTGGGCCTCCGCGTCTCTCCCGCCCACCGGAGGATGGGAATTGGGCTGAAGCTGGTGCGCGGAATGGAGGAGTGGTTCAGAGAGAATGGCGCCGAGTATTCGTACATAGCAACGGAAAACGACAACGTTGCTTCGGTTAATCTGTTCACCGAAAAATGCGGCTACTCCAAGTTCCGTACACCGGCCATCCTTGTCAATCCCGTGTTCGCCCACCGAGTCCCGCTGTCCCATCGCGTCACAATCCTCCCACTCTCACGCGCCGACGCCGAGATTCTCTATCGCCGCCGCTTCTCCACCACCGAGTTTTTCCCCCGCGACATTGACTCCGTCCTCGGTAACAAACTCACACTCGGCACCTTTCTCGCGCTGCCACGTGGCTCTTACTCCTGGACCGGGTCCGACCGCTTCCTGCTCGACCCGCCCGAGTCGTGGGCGGTTCTGAGTGTGTGGAACTGCAAGGACGTGTTCACGCTCCAGGTACGTGGAGCGTCACGCGTGAAGCGGACGTTGGCGAAAACGACACGCGTCTTGGATCGGGCGTTTCCGTGGCTGCGCCTGCCTTCGGTGCCGGAGCTGTTCAGGCCGTTCGGGCTGCACTTTCTGTACGGGCTGGGAGGGGAGGGCCCGGCGGCGGGGAAGTTGATGAAGGCGCTGTGTGGGTACGCGCACAATCTGGCCAAGGAGCGTGGGTGTGGGGTGGTGGCGACGGAGGTGTCGGCGAGGGAGCCGCTCCGGCCGGCGATTCCTCACTGGAAAATGCTGTCGTGCGAGGAGGATCTGTGGTGCATCAAGCGCCTCGGGGAAGACTATAGCGACGGCTCTGTCGGTGACTGGACCAAATCACCACCTGGCTTGTCGATTTTTGTCGACCCCAGAGAGTTC

Coding sequence (CDS)

GAAATAGTGATTAGAGAGTTCGATGCGAGTAAAGATGGCTTGGGGGTGGAAGATGTGGAGAGAAGGTGCGAAGTTGGACCAAGCGGCAAACTCTGTCTTTTCACCGACCTCTTGGGTGACCCAATTTGCAGGGTCCGCAATTCCCCTGCCTTCCTCATGCTGGTGGCGTCGACGGGCGACGAGAACGAGATACTGGGGATGATTAGAGGCTGCATCAAAACCGTTACATGCGGTAAAAAACTCTCCCGAACTCCCAAAACCACCACCCACGACGATCCCCCCTCCAAACACGTCCCCGTTTTCACTAAACTCGCCTACATCTTGGGCCTCCGCGTCTCTCCCGCCCACCGGAGGATGGGAATTGGGCTGAAGCTGGTGCGCGGAATGGAGGAGTGGTTCAGAGAGAATGGCGCCGAGTATTCGTACATAGCAACGGAAAACGACAACGTTGCTTCGGTTAATCTGTTCACCGAAAAATGCGGCTACTCCAAGTTCCGTACACCGGCCATCCTTGTCAATCCCGTGTTCGCCCACCGAGTCCCGCTGTCCCATCGCGTCACAATCCTCCCACTCTCACGCGCCGACGCCGAGATTCTCTATCGCCGCCGCTTCTCCACCACCGAGTTTTTCCCCCGCGACATTGACTCCGTCCTCGGTAACAAACTCACACTCGGCACCTTTCTCGCGCTGCCACGTGGCTCTTACTCCTGGACCGGGTCCGACCGCTTCCTGCTCGACCCGCCCGAGTCGTGGGCGGTTCTGAGTGTGTGGAACTGCAAGGACGTGTTCACGCTCCAGGTACGTGGAGCGTCACGCGTGAAGCGGACGTTGGCGAAAACGACACGCGTCTTGGATCGGGCGTTTCCGTGGCTGCGCCTGCCTTCGGTGCCGGAGCTGTTCAGGCCGTTCGGGCTGCACTTTCTGTACGGGCTGGGAGGGGAGGGCCCGGCGGCGGGGAAGTTGATGAAGGCGCTGTGTGGGTACGCGCACAATCTGGCCAAGGAGCGTGGGTGTGGGGTGGTGGCGACGGAGGTGTCGGCGAGGGAGCCGCTCCGGCCGGCGATTCCTCACTGGAAAATGCTGTCGTGCGAGGAGGATCTGTGGTGCATCAAGCGCCTCGGGGAAGACTATAGCGACGGCTCTGTCGGTGACTGGACCAAATCACCACCTGGCTTGTCGATTTTTGTCGACCCCAGAGAGTTC

Protein sequence

EIVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGDENEILGMIRGCIKTVTCGKKLSRTPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAHRRMGIGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFAHRVPLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSYSWTGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLPSVPELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPAIPHWKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF
Homology
BLAST of MS011492 vs. NCBI nr
Match: XP_023003005.1 (probable N-acetyltransferase HLS1 [Cucurbita maxima])

HSP 1 Score: 733.8 bits (1893), Expect = 8.0e-208
Identity = 351/404 (86.88%), Postives = 376/404 (93.07%), Query Frame = 0

Query: 1   EIVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGD 60
           +IVIREFDASKD +GVEDVERRCE+GPSGKLCLFTDLLGDPICRVRNSPAFLMLVA+TGD
Sbjct: 4   KIVIREFDASKDCVGVEDVERRCEIGPSGKLCLFTDLLGDPICRVRNSPAFLMLVAATGD 63

Query: 61  ENEILGMIRGCIKTVTCGKKLSRTPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAHRRMG 120
            ++ILGMIRGCIKTVTCGKKLSRTPKT   D  P+K+VPV+TKLAYILGLRVSP HRRMG
Sbjct: 64  HDDILGMIRGCIKTVTCGKKLSRTPKTAPADH-PTKYVPVYTKLAYILGLRVSPGHRRMG 123

Query: 121 IGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFAHRV 180
           IGLKLV+ MEEWFRENGAEYSYIATE DNVASVNLFTE CGYSKFRTPAILVNPVFAH +
Sbjct: 124 IGLKLVKKMEEWFRENGAEYSYIATEKDNVASVNLFTEXCGYSKFRTPAILVNPVFAHPL 183

Query: 181 PLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSY---SW 240
           PLSHRVTILPLSR+DAEILYR RFSTTEFFPRDIDS+L N+LTLGTFLA+PRG Y   +W
Sbjct: 184 PLSHRVTILPLSRSDAEILYRSRFSTTEFFPRDIDSILNNRLTLGTFLAIPRGVYTFDTW 243

Query: 241 TGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLPSVP 300
            GSDRFLL+PP SWAVLSVWNC DVF LQVRG S VKRTLA+TTRVLDRAFPWLRLPSVP
Sbjct: 244 PGSDRFLLNPPHSWAVLSVWNCNDVFRLQVRGVSLVKRTLARTTRVLDRAFPWLRLPSVP 303

Query: 301 ELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPAIPH 360
           ELF+PFGLHFLYGLGGEGP AG+++KALCGYAHNLAKERGCGVVATEVSARE +R AIPH
Sbjct: 304 ELFKPFGLHFLYGLGGEGPEAGRMVKALCGYAHNLAKERGCGVVATEVSARERVRAAIPH 363

Query: 361 WKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           WKMLSCEEDLWCI+RL ED+SDGSVGDWTKSPPGLSIFVDPREF
Sbjct: 364 WKMLSCEEDLWCIRRLTEDFSDGSVGDWTKSPPGLSIFVDPREF 406

BLAST of MS011492 vs. NCBI nr
Match: XP_023518402.1 (probable N-acetyltransferase HLS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 732.3 bits (1889), Expect = 2.3e-207
Identity = 352/407 (86.49%), Postives = 375/407 (92.14%), Query Frame = 0

Query: 1   EIVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGD 60
           +IVIREFDASKD +GVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVA+T D
Sbjct: 4   KIVIREFDASKDCMGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVAATAD 63

Query: 61  ENEILGMIRGCIKTVTCGKKLSRTPKTTTHD---DPPSKHVPVFTKLAYILGLRVSPAHR 120
            ++ILGMIRGCIKTVTCGKKLSRTPKTT H    D P+K+VPV+TKLAYILGLRVSPAHR
Sbjct: 64  HDDILGMIRGCIKTVTCGKKLSRTPKTTIHHAAADHPTKYVPVYTKLAYILGLRVSPAHR 123

Query: 121 RMGIGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFA 180
           RMGIGLKLV  MEEWFRENGAEYSYIATE DNVASVNLFTEKCGYSKFRTPAILVNPVFA
Sbjct: 124 RMGIGLKLVEKMEEWFRENGAEYSYIATEKDNVASVNLFTEKCGYSKFRTPAILVNPVFA 183

Query: 181 HRVPLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSY-- 240
           H +PLS RVTILPLSR+DAEILYR RFSTTEFFPRDIDS+L N LTLGTFLA+PRG Y  
Sbjct: 184 HPLPLSQRVTILPLSRSDAEILYRSRFSTTEFFPRDIDSILNNHLTLGTFLAIPRGVYTH 243

Query: 241 -SWTGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLP 300
            +W GSDRFLL+PP SWAVLSVWNC DVF LQVRG S VKRTLA+TTRVLDRAFPWLRLP
Sbjct: 244 DTWPGSDRFLLNPPHSWAVLSVWNCNDVFRLQVRGVSLVKRTLARTTRVLDRAFPWLRLP 303

Query: 301 SVPELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPA 360
           SVPELF+PFGLHFLYGLGGEGP AG+++K LCGYAHNLAKE+GCGVVATEVSARE +R A
Sbjct: 304 SVPELFKPFGLHFLYGLGGEGPEAGRMVKTLCGYAHNLAKEKGCGVVATEVSARERVRAA 363

Query: 361 IPHWKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           IPHWKMLSCEEDLWCI+RL ED+SDGSVGDWTKSPPGLSIFVDPREF
Sbjct: 364 IPHWKMLSCEEDLWCIRRLTEDFSDGSVGDWTKSPPGLSIFVDPREF 410

BLAST of MS011492 vs. NCBI nr
Match: KAG6604134.1 (putative N-acetyltransferase HLS1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 730.7 bits (1885), Expect = 6.8e-207
Identity = 352/406 (86.70%), Postives = 374/406 (92.12%), Query Frame = 0

Query: 2   IVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGDE 61
           +VIREFDASKD   VEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVA+TG+E
Sbjct: 8   MVIREFDASKDSKSVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVAATGEE 67

Query: 62  NEILGMIRGCIKTVTCGKKLSR---TPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAHRR 121
           NEI+GMIRGCIKTVTCG+KLSR    PKT  H    + H+PVFTKLAYILGLRVSPAHRR
Sbjct: 68  NEIVGMIRGCIKTVTCGQKLSRAPPNPKTHHHHHSTNHHLPVFTKLAYILGLRVSPAHRR 127

Query: 122 MGIGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFAH 181
           MGIG+KLV+ MEEWFRENGAEYSYIATE DNVASVNLFTEKC YSKFRTPAILVNPVFAH
Sbjct: 128 MGIGMKLVKKMEEWFRENGAEYSYIATEKDNVASVNLFTEKCDYSKFRTPAILVNPVFAH 187

Query: 182 RVPLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSY--- 241
            +P+S RVTILPLSRADAEILYRRRFSTTEFFPRDID++L N LTLGTFLA PRG+Y   
Sbjct: 188 PLPVSQRVTILPLSRADAEILYRRRFSTTEFFPRDIDAILNNVLTLGTFLAFPRGTYLPQ 247

Query: 242 SWTGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLPS 301
           +W GSDRFLLDPPESWAVLSVWNC DVF LQVRGASR+KR+LA+TTRVLDRAFPWLRLPS
Sbjct: 248 TWPGSDRFLLDPPESWAVLSVWNCNDVFKLQVRGASRLKRSLARTTRVLDRAFPWLRLPS 307

Query: 302 VPELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPAI 361
           VPELFRPFGLHFLYGLGGEGP AG+++KALCGYAHNLAKERGCGVVATEVSARE LR AI
Sbjct: 308 VPELFRPFGLHFLYGLGGEGPEAGRMVKALCGYAHNLAKERGCGVVATEVSARERLRGAI 367

Query: 362 PHWKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           PHWKMLSCEEDLWCIKRL ED+SDGSVGDWTKSPPGLSIFVDPREF
Sbjct: 368 PHWKMLSCEEDLWCIKRLTEDFSDGSVGDWTKSPPGLSIFVDPREF 413

BLAST of MS011492 vs. NCBI nr
Match: KAG7026942.1 (putative N-acetyltransferase HLS1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 728.4 bits (1879), Expect = 3.4e-206
Identity = 350/407 (86.00%), Postives = 374/407 (91.89%), Query Frame = 0

Query: 1   EIVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGD 60
           +IVIREFDASKD +GVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVA+T D
Sbjct: 4   KIVIREFDASKDCMGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVAATAD 63

Query: 61  ENEILGMIRGCIKTVTCGKKLSRTPKTTTHD---DPPSKHVPVFTKLAYILGLRVSPAHR 120
            ++ILGMIRGCIKTVTCGKKLSRTPKTT H    D P+K+VPV+TKLAYILGLRVSPAHR
Sbjct: 64  HDDILGMIRGCIKTVTCGKKLSRTPKTTLHHAAADHPTKYVPVYTKLAYILGLRVSPAHR 123

Query: 121 RMGIGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFA 180
           RMGIGLKLV+ MEEWFRENGAEYSYIATE DNVASVNLFTEKCGYSKFRTPAILVNPVFA
Sbjct: 124 RMGIGLKLVKKMEEWFRENGAEYSYIATEKDNVASVNLFTEKCGYSKFRTPAILVNPVFA 183

Query: 181 HRVPLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSY-- 240
           H +PLS RVTILPLSR+DAEILYR RFSTTEFFPRDIDS+L N LTLGTFLA+PRG Y  
Sbjct: 184 HPLPLSQRVTILPLSRSDAEILYRSRFSTTEFFPRDIDSILNNHLTLGTFLAIPRGVYTH 243

Query: 241 -SWTGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLP 300
            +W GSDRFLL+PP SWAVLSVWNC DVF LQVRG S VKRTLA+TTRVLDRAFPWLRLP
Sbjct: 244 DTWPGSDRFLLNPPHSWAVLSVWNCNDVFRLQVRGVSLVKRTLARTTRVLDRAFPWLRLP 303

Query: 301 SVPELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPA 360
           SVPELF+PFGLHFLYGLGGEGP  G+++K LCGYAHNLAKE+ CGVVATEVSARE +R A
Sbjct: 304 SVPELFKPFGLHFLYGLGGEGPEVGRMVKTLCGYAHNLAKEKECGVVATEVSARERVRAA 363

Query: 361 IPHWKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           IPHWKMLSCEEDLWCI+RL ED+SDGSVGDWTKSPPGLSIFVDPREF
Sbjct: 364 IPHWKMLSCEEDLWCIRRLTEDFSDGSVGDWTKSPPGLSIFVDPREF 410

BLAST of MS011492 vs. NCBI nr
Match: XP_023544685.1 (probable N-acetyltransferase HLS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 727.6 bits (1877), Expect = 5.7e-206
Identity = 351/406 (86.45%), Postives = 372/406 (91.63%), Query Frame = 0

Query: 2   IVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGDE 61
           IVIREFDASKD   VEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVA+TG+E
Sbjct: 8   IVIREFDASKDSKSVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVAATGEE 67

Query: 62  NEILGMIRGCIKTVTCGKKLSR---TPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAHRR 121
           NEI+GMIRGCIKTVTCG+KLSR    PKT  H    + H+PVFTKLAYILGLRVSPAHRR
Sbjct: 68  NEIVGMIRGCIKTVTCGQKLSRAPPNPKTHHHHHSTNHHLPVFTKLAYILGLRVSPAHRR 127

Query: 122 MGIGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFAH 181
           MGIG+KLV+ MEEWFRENGAEYSYIATE DNVASVNLFTEKC YSKFRTPAILVNPVFAH
Sbjct: 128 MGIGMKLVKKMEEWFRENGAEYSYIATEKDNVASVNLFTEKCDYSKFRTPAILVNPVFAH 187

Query: 182 RVPLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSY--- 241
            +P+S RVT+LPLSRADAEILYRRRFSTTEFFPRDID++L N LTLGTFLA PRG Y   
Sbjct: 188 PLPVSQRVTLLPLSRADAEILYRRRFSTTEFFPRDIDAILNNVLTLGTFLAFPRGIYTPQ 247

Query: 242 SWTGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLPS 301
           +W GSDRFLLDPPESWAVLSVWNC DVF LQVRGASR+KR+LA+TTRVLDRAFPWLRLPS
Sbjct: 248 TWPGSDRFLLDPPESWAVLSVWNCNDVFKLQVRGASRLKRSLARTTRVLDRAFPWLRLPS 307

Query: 302 VPELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPAI 361
           VPELFRPFGLHFLYGLGGEG  AG+++KALCGYAHNLAKERGCGVVATEVSARE LR AI
Sbjct: 308 VPELFRPFGLHFLYGLGGEGSEAGRMVKALCGYAHNLAKERGCGVVATEVSARERLRGAI 367

Query: 362 PHWKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           PHWKMLSCEEDLWCIKRL ED+SDGSVGDWTKSPPGLSIFVDPREF
Sbjct: 368 PHWKMLSCEEDLWCIKRLAEDFSDGSVGDWTKSPPGLSIFVDPREF 413

BLAST of MS011492 vs. ExPASy Swiss-Prot
Match: Q42381 (Probable N-acetyltransferase HLS1 OS=Arabidopsis thaliana OX=3702 GN=HLS1 PE=1 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 1.0e-173
Identity = 290/406 (71.43%), Postives = 338/406 (83.25%), Query Frame = 0

Query: 3   VIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGDE- 62
           V+RE+D ++D +GVEDVERRCEVGPSGKL LFTDLLGDPICR+R+SP++LMLVA  G E 
Sbjct: 3   VVREYDPTRDLVGVEDVERRCEVGPSGKLSLFTDLLGDPICRIRHSPSYLMLVAEMGTEK 62

Query: 63  NEILGMIRGCIKTVTCGKKLSRTPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAHRRMGI 122
            EI+GMIRGCIKTVTCG+KL        H        P++TKLAY+LGLRVSP HRR GI
Sbjct: 63  KEIVGMIRGCIKTVTCGQKLD-----LNHKSQNDVVKPLYTKLAYVLGLRVSPFHRRQGI 122

Query: 123 GLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFAHRVP 182
           G KLV+ MEEWFR+NGAEYSYIATENDN ASVNLFT KCGYS+FRTP+ILVNPV+AHRV 
Sbjct: 123 GFKLVKMMEEWFRQNGAEYSYIATENDNQASVNLFTGKCGYSEFRTPSILVNPVYAHRVN 182

Query: 183 LSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSY------ 242
           +S RVT++ L   DAE LYR RFSTTEFFPRDIDSVL NKL+LGTF+A+PRGS       
Sbjct: 183 VSRRVTVIKLEPVDAETLYRIRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCYGSGSG 242

Query: 243 SWTGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLPS 302
           SW GS +FL  PPESWAVLSVWNCKD F L+VRGASR++R +AKTTRV+D+  P+L+LPS
Sbjct: 243 SWPGSAKFLEYPPESWAVLSVWNCKDSFLLEVRGASRLRRVVAKTTRVVDKTLPFLKLPS 302

Query: 303 VPELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPAI 362
           +P +F PFGLHF+YG+GGEGP A K++K+LC +AHNLAK  GCGVVA EV+  +PLR  I
Sbjct: 303 IPSVFEPFGLHFMYGIGGEGPRAVKMVKSLCAHAHNLAKAGGCGVVAAEVAGEDPLRRGI 362

Query: 363 PHWKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           PHWK+LSC+EDLWCIKRLG+DYSDG VGDWTKSPPG+SIFVDPREF
Sbjct: 363 PHWKVLSCDEDLWCIKRLGDDYSDGVVGDWTKSPPGVSIFVDPREF 403

BLAST of MS011492 vs. ExPASy Swiss-Prot
Match: O64815 (Probable N-acetyltransferase HLS1-like OS=Arabidopsis thaliana OX=3702 GN=At2g23060 PE=2 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 1.6e-171
Identity = 288/411 (70.07%), Postives = 342/411 (83.21%), Query Frame = 0

Query: 2   IVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTG-- 61
           + +RE+D SKD   VEDVERRCEVGP+GKL LFTDLLGDPICRVR+SP++LMLVA  G  
Sbjct: 5   VEVREYDPSKDLATVEDVERRCEVGPAGKLSLFTDLLGDPICRVRHSPSYLMLVAEIGPK 64

Query: 62  DENEILGMIRGCIKTVTCG---KKLSRTPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAH 121
           ++ E++GMIRGCIKTVTCG   K+L  T   + +D   +K  P++TKLAYILGLRVSP H
Sbjct: 65  EKKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITK--PLYTKLAYILGLRVSPTH 124

Query: 122 RRMGIGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVF 181
           RR GIG KLV+ ME+WF +NGAEYSY ATENDN ASVNLFT KCGY++FRTP+ILVNPV+
Sbjct: 125 RRQGIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRTPSILVNPVY 184

Query: 182 AHRVPLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPR---- 241
           AHRV +S RVT++ L  +DAE+LYR RFSTTEFFPRDIDSVL NKL+LGTF+A+PR    
Sbjct: 185 AHRVNISRRVTVIKLEPSDAELLYRLRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCY 244

Query: 242 --GSYSWTGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPW 301
             GS SW GS +FL  PP+SWAVLSVWNCKD F L+VRGASR++R ++K TR++D+  P+
Sbjct: 245 GSGSRSWPGSAKFLEYPPDSWAVLSVWNCKDSFRLEVRGASRLRRVVSKATRMVDKTLPF 304

Query: 302 LRLPSVPELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREP 361
           L++PS+P +FRPFGLHF+YG+GGEGP A K++KALC +AHNLAKE GCGVVA EV+  EP
Sbjct: 305 LKIPSIPAVFRPFGLHFMYGIGGEGPRAEKMVKALCDHAHNLAKEGGCGVVAAEVAGEEP 364

Query: 362 LRPAIPHWKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           LR  IPHWK+LSC EDLWCIKRLGEDYSDGSVGDWTKSPPG SIFVDPREF
Sbjct: 365 LRRGIPHWKVLSCAEDLWCIKRLGEDYSDGSVGDWTKSPPGDSIFVDPREF 413

BLAST of MS011492 vs. ExPASy TrEMBL
Match: A0A6J1KQK4 (probable N-acetyltransferase HLS1 OS=Cucurbita maxima OX=3661 GN=LOC111496749 PE=4 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 3.9e-208
Identity = 351/404 (86.88%), Postives = 376/404 (93.07%), Query Frame = 0

Query: 1   EIVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGD 60
           +IVIREFDASKD +GVEDVERRCE+GPSGKLCLFTDLLGDPICRVRNSPAFLMLVA+TGD
Sbjct: 4   KIVIREFDASKDCVGVEDVERRCEIGPSGKLCLFTDLLGDPICRVRNSPAFLMLVAATGD 63

Query: 61  ENEILGMIRGCIKTVTCGKKLSRTPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAHRRMG 120
            ++ILGMIRGCIKTVTCGKKLSRTPKT   D  P+K+VPV+TKLAYILGLRVSP HRRMG
Sbjct: 64  HDDILGMIRGCIKTVTCGKKLSRTPKTAPADH-PTKYVPVYTKLAYILGLRVSPGHRRMG 123

Query: 121 IGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFAHRV 180
           IGLKLV+ MEEWFRENGAEYSYIATE DNVASVNLFTE CGYSKFRTPAILVNPVFAH +
Sbjct: 124 IGLKLVKKMEEWFRENGAEYSYIATEKDNVASVNLFTEXCGYSKFRTPAILVNPVFAHPL 183

Query: 181 PLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSY---SW 240
           PLSHRVTILPLSR+DAEILYR RFSTTEFFPRDIDS+L N+LTLGTFLA+PRG Y   +W
Sbjct: 184 PLSHRVTILPLSRSDAEILYRSRFSTTEFFPRDIDSILNNRLTLGTFLAIPRGVYTFDTW 243

Query: 241 TGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLPSVP 300
            GSDRFLL+PP SWAVLSVWNC DVF LQVRG S VKRTLA+TTRVLDRAFPWLRLPSVP
Sbjct: 244 PGSDRFLLNPPHSWAVLSVWNCNDVFRLQVRGVSLVKRTLARTTRVLDRAFPWLRLPSVP 303

Query: 301 ELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPAIPH 360
           ELF+PFGLHFLYGLGGEGP AG+++KALCGYAHNLAKERGCGVVATEVSARE +R AIPH
Sbjct: 304 ELFKPFGLHFLYGLGGEGPEAGRMVKALCGYAHNLAKERGCGVVATEVSARERVRAAIPH 363

Query: 361 WKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           WKMLSCEEDLWCI+RL ED+SDGSVGDWTKSPPGLSIFVDPREF
Sbjct: 364 WKMLSCEEDLWCIRRLTEDFSDGSVGDWTKSPPGLSIFVDPREF 406

BLAST of MS011492 vs. ExPASy TrEMBL
Match: A0A6J1GEX3 (probable N-acetyltransferase HLS1 OS=Cucurbita moschata OX=3662 GN=LOC111453566 PE=4 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 3.6e-206
Identity = 351/407 (86.24%), Postives = 371/407 (91.15%), Query Frame = 0

Query: 2   IVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGDE 61
           IVIREFDASKD   VEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVA+TG+E
Sbjct: 8   IVIREFDASKDSKSVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVAATGEE 67

Query: 62  NEILGMIRGCIKTVTCGKKLSRTPKTTT----HDDPPSKHVPVFTKLAYILGLRVSPAHR 121
           NEI+GMIRGCIKTVTCG+KLSR P        H    + H+PVFTKLAYILGLRVSPAHR
Sbjct: 68  NEIVGMIRGCIKTVTCGQKLSRAPPNPKTHHHHHHSTNHHLPVFTKLAYILGLRVSPAHR 127

Query: 122 RMGIGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFA 181
           RMGIG+KLV+ MEEWFRENGAEYSYIATE DNVASVNLFTEKC YSKFRTPAILVNPVFA
Sbjct: 128 RMGIGMKLVKKMEEWFRENGAEYSYIATEKDNVASVNLFTEKCDYSKFRTPAILVNPVFA 187

Query: 182 HRVPLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSY-- 241
           H +P+S RVTILPLSRADAEILYRRRFSTTEFFPRDID++L N LTLGTFLA PRG+Y  
Sbjct: 188 HPLPVSQRVTILPLSRADAEILYRRRFSTTEFFPRDIDAILNNVLTLGTFLAFPRGTYLP 247

Query: 242 -SWTGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLP 301
            +W GSDRFLLDPPESWAVLSVWNC DVF LQVRGASR+KR+LA+TTRVLDRAFPWLRLP
Sbjct: 248 QTWPGSDRFLLDPPESWAVLSVWNCNDVFKLQVRGASRLKRSLARTTRVLDRAFPWLRLP 307

Query: 302 SVPELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPA 361
           SVPELFRPFGLHFLYGLGGEGP AG ++KALCGYAHNLAKERGCGVVATEVSARE LR A
Sbjct: 308 SVPELFRPFGLHFLYGLGGEGPEAGGMVKALCGYAHNLAKERGCGVVATEVSARERLRGA 367

Query: 362 IPHWKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           IPHWKMLSCEEDLWCIKRL ED+SDGSVGDWTKSPPGLSIFVDPREF
Sbjct: 368 IPHWKMLSCEEDLWCIKRLAEDFSDGSVGDWTKSPPGLSIFVDPREF 414

BLAST of MS011492 vs. ExPASy TrEMBL
Match: A0A6J1HF75 (probable N-acetyltransferase HLS1 OS=Cucurbita moschata OX=3662 GN=LOC111463421 PE=4 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 4.7e-206
Identity = 349/404 (86.39%), Postives = 373/404 (92.33%), Query Frame = 0

Query: 1   EIVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGD 60
           +IVIREFDASKD +GVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVA+T D
Sbjct: 18  KIVIREFDASKDCVGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVAATAD 77

Query: 61  ENEILGMIRGCIKTVTCGKKLSRTPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAHRRMG 120
            ++ILGMIRGCIKTVTCGKKLSRTPK    D  P+K+VPV+TKLAYILGLRVSPAHRRMG
Sbjct: 78  HDDILGMIRGCIKTVTCGKKLSRTPKAVPADH-PTKYVPVYTKLAYILGLRVSPAHRRMG 137

Query: 121 IGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFAHRV 180
           IGLKLV+ MEEWFRENGAEYSYIATE DNVASVNLFTEKCGYSKFRTPAILVNPVFAH +
Sbjct: 138 IGLKLVKKMEEWFRENGAEYSYIATEKDNVASVNLFTEKCGYSKFRTPAILVNPVFAHPL 197

Query: 181 PLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSY---SW 240
           PLS RVTILPLSR+DAEILYR RFSTTEFFPRDIDS+L N LTLGTFLA+PRG Y   +W
Sbjct: 198 PLSQRVTILPLSRSDAEILYRSRFSTTEFFPRDIDSILNNHLTLGTFLAIPRGVYTHDTW 257

Query: 241 TGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLPSVP 300
            GSDRFLL+PP SWAVLSVWNC DVF LQVRG S VKRTLA+TTRVLDRAFPWLRLPSVP
Sbjct: 258 PGSDRFLLNPPHSWAVLSVWNCNDVFRLQVRGVSLVKRTLARTTRVLDRAFPWLRLPSVP 317

Query: 301 ELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPAIPH 360
           ELF+PFGLHFLYGLGGEGP AG+++K LCGYAHNLAKE+GCGVVATEVSARE +R AIPH
Sbjct: 318 ELFKPFGLHFLYGLGGEGPEAGRMVKTLCGYAHNLAKEKGCGVVATEVSARERVRAAIPH 377

Query: 361 WKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           WKMLSCEEDLWCI+RL ED+SDGSVGDWTKSPPGLSIFVDPREF
Sbjct: 378 WKMLSCEEDLWCIRRLTEDFSDGSVGDWTKSPPGLSIFVDPREF 420

BLAST of MS011492 vs. ExPASy TrEMBL
Match: A0A6J1IJC0 (probable N-acetyltransferase HLS1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477959 PE=4 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 7.5e-204
Identity = 349/404 (86.39%), Postives = 370/404 (91.58%), Query Frame = 0

Query: 2   IVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGDE 61
           IVIREFDASKD  GVEDVERRC VGPSGKLCLFTDLLGDPICRVRNSPAFLMLVA+TG+E
Sbjct: 8   IVIREFDASKDSKGVEDVERRCAVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVAATGEE 67

Query: 62  NEILGMIRGCIKTVTCGKKLSRT-PKTTTHDDPPSKHVPVFTKLAYILGLRVSPAHRRMG 121
           NEI+GMIRGCIKTVTCG+KLSR  P   TH      H+PVFTKLAYILGLRVSPAHRRMG
Sbjct: 68  NEIVGMIRGCIKTVTCGQKLSRAPPNPKTH-----HHLPVFTKLAYILGLRVSPAHRRMG 127

Query: 122 IGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFAHRV 181
           IG+KLV+ MEEWFRENGAEYSYIATE DNVASVNLFTEKC YSKFRTPAILVNPVFA  +
Sbjct: 128 IGMKLVKKMEEWFRENGAEYSYIATEKDNVASVNLFTEKCDYSKFRTPAILVNPVFARPL 187

Query: 182 PLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSY---SW 241
           P+S RVTILPLSRADAEILYRRRFSTTEFFPRDID++L N LTLGTFLA PRG+Y   +W
Sbjct: 188 PVSQRVTILPLSRADAEILYRRRFSTTEFFPRDIDAILDNVLTLGTFLAFPRGTYTPQTW 247

Query: 242 TGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLPSVP 301
            GSDRFLLDPPESWAVLSVWNC DVF LQVRGASR+KR+LA+TTRVLD+AFPWLRLPSVP
Sbjct: 248 PGSDRFLLDPPESWAVLSVWNCNDVFKLQVRGASRLKRSLARTTRVLDKAFPWLRLPSVP 307

Query: 302 ELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPAIPH 361
           ELFRPFGLHFLYGLGGEGP AG+++KALCGYAHNLAKERGCGVVATEVSARE LR AIPH
Sbjct: 308 ELFRPFGLHFLYGLGGEGPEAGRMVKALCGYAHNLAKERGCGVVATEVSARERLRGAIPH 367

Query: 362 WKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           WKMLSCEEDLWCIKRL ED+SDGSVGDWTKS PGLSIFVDPREF
Sbjct: 368 WKMLSCEEDLWCIKRLAEDFSDGSVGDWTKSAPGLSIFVDPREF 406

BLAST of MS011492 vs. ExPASy TrEMBL
Match: A0A0A0KLH9 (N-acetyltransferase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G495060 PE=4 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 7.1e-202
Identity = 340/403 (84.37%), Postives = 370/403 (91.81%), Query Frame = 0

Query: 2   IVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGDE 61
           IVIREFD SKD + VEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVA+T D+
Sbjct: 2   IVIREFDPSKDCIAVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVAATADQ 61

Query: 62  NEILGMIRGCIKTVTCGKKLSRTPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAHRRMGI 121
           NEI+GMIRGCIKTVTCG+KLSR+    +   PP KH+PV+TKLAYILGLRVSPAHRRMGI
Sbjct: 62  NEIVGMIRGCIKTVTCGQKLSRSAIPNSDHQPP-KHLPVYTKLAYILGLRVSPAHRRMGI 121

Query: 122 GLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFAHRVP 181
           G+KLV+ MEEWFRE+GAEYSYIATE DNVASVNLFTEKC YSKFRTPAILVNPVFAH VP
Sbjct: 122 GIKLVKKMEEWFRESGAEYSYIATEKDNVASVNLFTEKCEYSKFRTPAILVNPVFAHPVP 181

Query: 182 LSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSY---SWT 241
           LS RVTILPLSR+DAEILYRRRFSTTEFFPRDID+VL N LTLGTFLA+PRG+Y   +W 
Sbjct: 182 LSKRVTILPLSRSDAEILYRRRFSTTEFFPRDIDAVLNNPLTLGTFLAIPRGTYTPHTWP 241

Query: 242 GSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLPSVPE 301
           GSDRFL+DPP+SWAVLSVWNC DVF LQVRG SR+KR+ A+TTRVLD+AFPWLRLPSVPE
Sbjct: 242 GSDRFLVDPPQSWAVLSVWNCNDVFRLQVRGVSRLKRSFARTTRVLDKAFPWLRLPSVPE 301

Query: 302 LFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPAIPHW 361
           LF PFGLHF+YGLGGEGP A +++KALCGYAHNLAKE+GCGVVATEVSA E LR AIPHW
Sbjct: 302 LFSPFGLHFMYGLGGEGPDAERMLKALCGYAHNLAKEKGCGVVATEVSAGERLRTAIPHW 361

Query: 362 KMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           KMLSCEEDLWCIKRLGED+SDGSVGDWTKSPPG+SIFVDPREF
Sbjct: 362 KMLSCEEDLWCIKRLGEDFSDGSVGDWTKSPPGMSIFVDPREF 403

BLAST of MS011492 vs. TAIR 10
Match: AT4G37580.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 610.9 bits (1574), Expect = 7.3e-175
Identity = 290/406 (71.43%), Postives = 338/406 (83.25%), Query Frame = 0

Query: 3   VIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGDE- 62
           V+RE+D ++D +GVEDVERRCEVGPSGKL LFTDLLGDPICR+R+SP++LMLVA  G E 
Sbjct: 3   VVREYDPTRDLVGVEDVERRCEVGPSGKLSLFTDLLGDPICRIRHSPSYLMLVAEMGTEK 62

Query: 63  NEILGMIRGCIKTVTCGKKLSRTPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAHRRMGI 122
            EI+GMIRGCIKTVTCG+KL        H        P++TKLAY+LGLRVSP HRR GI
Sbjct: 63  KEIVGMIRGCIKTVTCGQKLD-----LNHKSQNDVVKPLYTKLAYVLGLRVSPFHRRQGI 122

Query: 123 GLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFAHRVP 182
           G KLV+ MEEWFR+NGAEYSYIATENDN ASVNLFT KCGYS+FRTP+ILVNPV+AHRV 
Sbjct: 123 GFKLVKMMEEWFRQNGAEYSYIATENDNQASVNLFTGKCGYSEFRTPSILVNPVYAHRVN 182

Query: 183 LSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSY------ 242
           +S RVT++ L   DAE LYR RFSTTEFFPRDIDSVL NKL+LGTF+A+PRGS       
Sbjct: 183 VSRRVTVIKLEPVDAETLYRIRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCYGSGSG 242

Query: 243 SWTGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLPS 302
           SW GS +FL  PPESWAVLSVWNCKD F L+VRGASR++R +AKTTRV+D+  P+L+LPS
Sbjct: 243 SWPGSAKFLEYPPESWAVLSVWNCKDSFLLEVRGASRLRRVVAKTTRVVDKTLPFLKLPS 302

Query: 303 VPELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPAI 362
           +P +F PFGLHF+YG+GGEGP A K++K+LC +AHNLAK  GCGVVA EV+  +PLR  I
Sbjct: 303 IPSVFEPFGLHFMYGIGGEGPRAVKMVKSLCAHAHNLAKAGGCGVVAAEVAGEDPLRRGI 362

Query: 363 PHWKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           PHWK+LSC+EDLWCIKRLG+DYSDG VGDWTKSPPG+SIFVDPREF
Sbjct: 363 PHWKVLSCDEDLWCIKRLGDDYSDGVVGDWTKSPPGVSIFVDPREF 403

BLAST of MS011492 vs. TAIR 10
Match: AT2G23060.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 603.6 bits (1555), Expect = 1.2e-172
Identity = 288/411 (70.07%), Postives = 342/411 (83.21%), Query Frame = 0

Query: 2   IVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTG-- 61
           + +RE+D SKD   VEDVERRCEVGP+GKL LFTDLLGDPICRVR+SP++LMLVA  G  
Sbjct: 5   VEVREYDPSKDLATVEDVERRCEVGPAGKLSLFTDLLGDPICRVRHSPSYLMLVAEIGPK 64

Query: 62  DENEILGMIRGCIKTVTCG---KKLSRTPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAH 121
           ++ E++GMIRGCIKTVTCG   K+L  T   + +D   +K  P++TKLAYILGLRVSP H
Sbjct: 65  EKKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITK--PLYTKLAYILGLRVSPTH 124

Query: 122 RRMGIGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVF 181
           RR GIG KLV+ ME+WF +NGAEYSY ATENDN ASVNLFT KCGY++FRTP+ILVNPV+
Sbjct: 125 RRQGIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRTPSILVNPVY 184

Query: 182 AHRVPLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPR---- 241
           AHRV +S RVT++ L  +DAE+LYR RFSTTEFFPRDIDSVL NKL+LGTF+A+PR    
Sbjct: 185 AHRVNISRRVTVIKLEPSDAELLYRLRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCY 244

Query: 242 --GSYSWTGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPW 301
             GS SW GS +FL  PP+SWAVLSVWNCKD F L+VRGASR++R ++K TR++D+  P+
Sbjct: 245 GSGSRSWPGSAKFLEYPPDSWAVLSVWNCKDSFRLEVRGASRLRRVVSKATRMVDKTLPF 304

Query: 302 LRLPSVPELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREP 361
           L++PS+P +FRPFGLHF+YG+GGEGP A K++KALC +AHNLAKE GCGVVA EV+  EP
Sbjct: 305 LKIPSIPAVFRPFGLHFMYGIGGEGPRAEKMVKALCDHAHNLAKEGGCGVVAAEVAGEEP 364

Query: 362 LRPAIPHWKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           LR  IPHWK+LSC EDLWCIKRLGEDYSDGSVGDWTKSPPG SIFVDPREF
Sbjct: 365 LRRGIPHWKVLSCAEDLWCIKRLGEDYSDGSVGDWTKSPPGDSIFVDPREF 413

BLAST of MS011492 vs. TAIR 10
Match: AT2G23060.2 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 521.9 bits (1343), Expect = 4.4e-148
Identity = 250/360 (69.44%), Postives = 297/360 (82.50%), Query Frame = 0

Query: 53  MLVASTG--DENEILGMIRGCIKTVTCG---KKLSRTPKTTTHDDPPSKHVPVFTKLAYI 112
           MLVA  G  ++ E++GMIRGCIKTVTCG   K+L  T   + +D   +K  P++TKLAYI
Sbjct: 1   MLVAEIGPKEKKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITK--PLYTKLAYI 60

Query: 113 LGLRVSPAHRRMGIGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRT 172
           LGLRVSP HRR GIG KLV+ ME+WF +NGAEYSY ATENDN ASVNLFT KCGY++FRT
Sbjct: 61  LGLRVSPTHRRQGIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRT 120

Query: 173 PAILVNPVFAHRVPLSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTF 232
           P+ILVNPV+AHRV +S RVT++ L  +DAE+LYR RFSTTEFFPRDIDSVL NKL+LGTF
Sbjct: 121 PSILVNPVYAHRVNISRRVTVIKLEPSDAELLYRLRFSTTEFFPRDIDSVLNNKLSLGTF 180

Query: 233 LALPR------GSYSWTGSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTT 292
           +A+PR      GS SW GS +FL  PP+SWAVLSVWNCKD F L+VRGASR++R ++K T
Sbjct: 181 VAVPRGSCYGSGSRSWPGSAKFLEYPPDSWAVLSVWNCKDSFRLEVRGASRLRRVVSKAT 240

Query: 293 RVLDRAFPWLRLPSVPELFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVV 352
           R++D+  P+L++PS+P +FRPFGLHF+YG+GGEGP A K++KALC +AHNLAKE GCGVV
Sbjct: 241 RMVDKTLPFLKIPSIPAVFRPFGLHFMYGIGGEGPRAEKMVKALCDHAHNLAKEGGCGVV 300

Query: 353 ATEVSAREPLRPAIPHWKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 402
           A EV+  EPLR  IPHWK+LSC EDLWCIKRLGEDYSDGSVGDWTKSPPG SIFVDPREF
Sbjct: 301 AAEVAGEEPLRRGIPHWKVLSCAEDLWCIKRLGEDYSDGSVGDWTKSPPGDSIFVDPREF 358

BLAST of MS011492 vs. TAIR 10
Match: AT5G67430.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 471.9 bits (1213), Expect = 5.3e-133
Identity = 242/399 (60.65%), Postives = 296/399 (74.19%), Query Frame = 0

Query: 2   IVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGDE 61
           +V+RE+D  +D   VE++E  CEVG      L  DL+GDP+ R+R SP+F MLVA  G  
Sbjct: 8   VVVREYDPKRDLTSVEELEESCEVG-----SLLVDLMGDPLARIRQSPSFHMLVAEIG-- 67

Query: 62  NEILGMIRGCIKTVTCGKKLSRTPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAHRRMGI 121
           NEI+GMIRG IK VT G    R        D  S  +   TKLA++ GLRVSP +RRMGI
Sbjct: 68  NEIVGMIRGTIKMVTRGVNALR------QADDVSPEINT-TKLAFVSGLRVSPFYRRMGI 127

Query: 122 GLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFAHRVP 181
           GLKLV+ +EEWF  N A YSY+ TENDN+ASV LFTEK GYSKFRTP  LVNPVF HRV 
Sbjct: 128 GLKLVQRLEEWFLRNDAVYSYVQTENDNIASVKLFTEKSGYSKFRTPTFLVNPVFNHRVT 187

Query: 182 LSHRVTILPLSRADAEILYRRRFSTTEFFPRDIDSVLGNKLTLGTFLALPRGSYSWTGSD 241
           +S RV I+ L+ +DAE LYR RFSTTEFFP DI+S+L NKL+LGT+LA+PRG  + +GS 
Sbjct: 188 VSRRVKIIKLAPSDAESLYRNRFSTTEFFPSDINSILTNKLSLGTYLAVPRGGDNVSGS- 247

Query: 242 RFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLPSVPELFR 301
             L D   SWAV+S+WN KDV+ LQV+GASR+KR LAK+TRV D AFP+L++PS P LF+
Sbjct: 248 --LPDQTGSWAVISIWNSKDVYRLQVKGASRLKRMLAKSTRVFDGAFPFLKIPSFPNLFK 307

Query: 302 PFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKERGCGVVATEVSAREPLRPAIPHWKML 361
            F +HF+YG+GGEGP A ++++ALC +AHNLA++ GC VVA EV++ EPLR  IPHWK+L
Sbjct: 308 SFAMHFMYGIGGEGPRAAEMVEALCSHAHNLARKSGCAVVAAEVASCEPLRVGIPHWKVL 367

Query: 362 SCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPRE 401
           S  EDLWC+KRL   Y D  V DWTKSPPGLSIFVDPRE
Sbjct: 368 S-PEDLWCLKRL--RYDDDGV-DWTKSPPGLSIFVDPRE 385

BLAST of MS011492 vs. TAIR 10
Match: AT2G30090.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.8e-83
Identity = 175/409 (42.79%), Postives = 240/409 (58.68%), Query Frame = 0

Query: 1   EIVIREFDASKDGLGVEDVERRCEVGPSGKLCLFTDLLGDPICRVRNSPAFLMLVASTGD 60
           E+VIR +D  +D + +  +E+ CE+G   +  LFTD LGDPICR+RNSP F+MLVA  G 
Sbjct: 12  EVVIRCYDDRRDRIQMGRMEKSCEIGHDHQTLLFTDTLGDPICRIRNSPFFIMLVAGVG- 71

Query: 61  ENEILGMIRGCIKTVTCGKKLSRTPKTTTHDDPPSKHVPVFTKLAYILGLRVSPAHRRMG 120
            N+++G I+G +K V              HD           ++ Y+LGLRV P++RR G
Sbjct: 72  -NKLVGSIQGSVKPV------------EFHDKS--------VRVGYVLGLRVVPSYRRRG 131

Query: 121 IGLKLVRGMEEWFRENGAEYSYIATENDNVASVNLFTEKCGYSKFRTPAILVNPVFAHR- 180
           IG  LVR +EEWF  + A+Y+Y+ATE DN AS  LF  + GY  FR PAILVNPV   R 
Sbjct: 132 IGSILVRKLEEWFESHNADYAYMATEKDNEASHGLFIGRLGYVVFRNPAILVNPVNPGRG 191

Query: 181 VPLSHRVTILPLSRADAEILYRRRF-STTEFFPRDIDSVLGNKLTLGTFLALPRGSYSWT 240
           + L   + I  L   +AE LYRR   +TTEFFP DI+ +L NKL++GT++A         
Sbjct: 192 LKLPSDIGIRKLKVKEAESLYRRNVAATTEFFPDDINKILRNKLSIGTWVAYYNN----- 251

Query: 241 GSDRFLLDPPESWAVLSVWNCKDVFTLQVRGASRVKRTLAKTTRVLDRAFPWLRLPSVPE 300
                 +D   SWA+LSVW+   VF L++  A      L K +++       L L  +P+
Sbjct: 252 ------VDNTRSWAMLSVWDSSKVFKLRIERAPLSYLLLTKVSKLFGNFLSLLGLTVLPD 311

Query: 301 LFRPFGLHFLYGLGGEGPAAGKLMKALCGYAHNLAKER---GCGVVATEV----SAREPL 360
           LF PFG +FLYG+  EGP  GKL++ALC + HN+A       C VV  EV    +  + L
Sbjct: 312 LFTPFGFYFLYGVHSEGPHCGKLVRALCEHVHNMAALNDGCACKVVVVEVDKGSNGDDSL 371

Query: 361 RPAIPHWKMLSCEEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPRE 401
           +  IPHWKMLSC++D+WCIK L  + +   + + +KS    S+FVDPRE
Sbjct: 372 QRCIPHWKMLSCDDDMWCIKPLKCEKNKFDLSERSKSRS--SLFVDPRE 385

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023003005.18.0e-20886.88probable N-acetyltransferase HLS1 [Cucurbita maxima][more]
XP_023518402.12.3e-20786.49probable N-acetyltransferase HLS1 [Cucurbita pepo subsp. pepo][more]
KAG6604134.16.8e-20786.70putative N-acetyltransferase HLS1-like protein, partial [Cucurbita argyrosperma ... [more]
KAG7026942.13.4e-20686.00putative N-acetyltransferase HLS1-like protein, partial [Cucurbita argyrosperma ... [more]
XP_023544685.15.7e-20686.45probable N-acetyltransferase HLS1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q423811.0e-17371.43Probable N-acetyltransferase HLS1 OS=Arabidopsis thaliana OX=3702 GN=HLS1 PE=1 S... [more]
O648151.6e-17170.07Probable N-acetyltransferase HLS1-like OS=Arabidopsis thaliana OX=3702 GN=At2g23... [more]
Match NameE-valueIdentityDescription
A0A6J1KQK43.9e-20886.88probable N-acetyltransferase HLS1 OS=Cucurbita maxima OX=3661 GN=LOC111496749 PE... [more]
A0A6J1GEX33.6e-20686.24probable N-acetyltransferase HLS1 OS=Cucurbita moschata OX=3662 GN=LOC111453566 ... [more]
A0A6J1HF754.7e-20686.39probable N-acetyltransferase HLS1 OS=Cucurbita moschata OX=3662 GN=LOC111463421 ... [more]
A0A6J1IJC07.5e-20486.39probable N-acetyltransferase HLS1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A0A0KLH97.1e-20284.37N-acetyltransferase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_... [more]
Match NameE-valueIdentityDescription
AT4G37580.17.3e-17571.43Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
AT2G23060.11.2e-17270.07Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
AT2G23060.24.4e-14869.44Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
AT5G67430.15.3e-13360.65Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
AT2G30090.13.8e-8342.79Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000182GNAT domainPFAMPF00583Acetyltransf_1coord: 55..162
e-value: 6.3E-17
score: 61.9
IPR000182GNAT domainPROSITEPS51186GNATcoord: 2..187
score: 13.571998
NoneNo IPR availableGENE3D3.40.630.30coord: 41..168
e-value: 4.8E-15
score: 57.7
NoneNo IPR availablePANTHERPTHR43072N-ACETYLTRANSFERASEcoord: 2..401
NoneNo IPR availablePANTHERPTHR43072:SF42N-ACETYLTRANSFERASE HLS1-LIKE-RELATEDcoord: 2..401
NoneNo IPR availableCDDcd04301NAT_SFcoord: 105..140
e-value: 5.6686E-7
score: 44.5741
IPR016181Acyl-CoA N-acyltransferaseSUPERFAMILY55729Acyl-CoA N-acyltransferases (Nat)coord: 42..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS011492.1MS011492.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0008080 N-acetyltransferase activity