MS011484 (gene) Bitter gourd (TR) v1

Overview
NameMS011484
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionribonuclease III domain-containing protein RNC1, chloroplastic
Locationscaffold239: 2305800 .. 2309171 (-)
RNA-Seq ExpressionMS011484
SyntenyMS011484
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACTTTCTTCTTCTTTTGTACAACTCGCATCCCCTACTCCTAATTACTCTTTCTCGTCTTCCTTAACCCCATTTCATTTCAAAATTTACCCCAATAATCCCAGTTTCCCGAACCCCACGTCGGGAAATTTGCGTATTTTTGCCGTTGCAGTTGACCCACAGCAAGAACTGCCCAAAAACAACCCCCAGAGACTGCTCAAGGAGCTCGCGGAGCGAAAACGAGCTACTTCTCCGAAGAAGAGGGTGCCCCCAAGAAGGTTTATTCTTAGACCGCCATTAGATGATAAGAAGTTAGCGGAAAGGTTTCTCAATAGCCCTCAATTAACCCTCAAATCATTCCCATTATTGAGTTCTTGTTTGCCATCTTCGCGGCTTAACAATGCCGATAAAACTTGGATGGACGAGTACTTGCTCGAAGCCAAGCAGGCTCTTGGATACCCCCTCGAGCCATCCGATAATTATGGAGACGACAATCCTGCGAAACAGTTTGACACTTTGTTGTACTTGGCCTTTCAGCATCCCTCGTGCGAGAGAACAAAGGCACGGCACATAAGATCGGGGCACTCAAGGCTACTGTTTTTGGGCCAATATGTGCTCGAATTGGCGTTAGCAGAGTATTTTCTGCAGAGATATCCTAGGGAATCGCCGGGTCCAATGAGGGAGCGAGTTTTTGCGTTAATTGGGAAGAGGAGCCTCCCAAAGTGGATTAAAGCTGCCAGTTTACAGAATTTGATATTCCCATACGATGATATGGATAAGATGATCAGGAAGGACCGTGAACCACCTGTGAAGTAAGTTTGGTATTGTCTTTTCATTGGTCTTAATTCTTCTTGTTCCCGATCTGAAATGCTGCTATAAATGAGATATTTTGTAAGTAATCTTTTTTGGGTTTAAGTGATTGAAAATTTGTGCCATTGATGTATTAAGCCCATGTTAAGTTTGAGAGGCATTGCAAAATGCGAGATCATCAGAGTTAGAAGTACCAAATTCTTTAGGGAAGCTGATTATGCAATATGATGATAATGCATTGTATAATTGATTAGACTTAATGGGGTATAAGAGGCATTGGGAAGGTCACATTTGATTAGAGTGAAGTCAAAAGTGCAGTTAACTACATTCCTGCCCCTGGAAATCAAAAGTTAGGTTAGCTAAGTTGTGATGGTTGTAGGCACTGGTTGAAGACGAGGCTACATAAAACCAACCAGAGACAATTTCAAGCTAGTTGCCAGTGAAAATGTGGCACATAGCATTAATTTGAAGTTCTTTAGGATTTTTATAGGTGGCTTCGTGTCATGGGGTTGATTCTTTGTTGACCTCAACTTTTGAGGTCCTAGCACCACACGTTCTCTACTTGAACCTTGACCTGTGGGTTATGGACTCCTTCCACGAATCTGGCTTCCTTCCAAATTCATTGCTCCTTTGTAAAAACTTGTTTCCCCATCAATAAATCTAGTTTTTAACAACTTGGCTTTCCATTTGCCCCTTTTTGGAAAATCTGTTGATACCACCACTTGTCATGGGTTTGAACATGGGTTACAGAGTTGGCGCTCATGGGTCTGTCACATTTTGTAATGCCTGCCTTGATTATGCAACATGATGAGAAGTACTGCCCTATGACACTTGGTCTATGGTGGGACTTGGAAGATTGGATTTTGTGTACCCAGTTATGTACTTCCCATGGTCATGGTCTATCAAATTCACATTTTTTTATGGTACAGCATGTGCTAGGTTACTGTAGTGAAACATGTATTGTTAATTTTCTTTTTCCAAACTCTGGTTAACTTATGGGATGTTTGACCCAGTATAATTTTGTTTTCTTTTCCTTTCATTGAACCATGGTATTGTTTTCCCTTGTATTTCTGAGTGAACTATCTTACACTTAATTTTTAGATTAGTGACTCCAGGGAACTGAAACCCCTACAGTTTCCCTTTTCTAAATAGTTTGATTCAACTGATTATATAAAAAAATTTGAGTCTAAAAGCAATTTTCTTCAAGCAGATCTGTGTTCTGGGCGTTGTTTGGGGCAATATACTTATGTTTTGGAATGCCAGAAGTGTATCGGGTCCTTTTTGAGGTGTTTGGAATGGATCCAGATGCAGAGGAATGTGAACCGAGGTTACGTAGGCAACTTGAAGATGTAGATTATGTCTCTGTTGAATTTGAGGACAAAAAGATCAGCTGGCAAGATATGGTTGCCTATAAGGCAAGTTTCATGTTTATTCTTAATAGCACAGTGAACTATATTTTATGCTATTGTTTTCATCTTTTTTTTTGGCATGAAATTGACTCTTGTGTTCATGTTTTCTTTCCCTCCTTCCTTTCTCTTCAGCCTCCCGAAGATGCTCTATTTGCACACCCTAGGCTCTTCAGAGCTTGTGTCCCACCTGGCATGCACAGATTCCGGGGAAATATATGGGATTACGATAGCCGACCACAAGTCATGCAAGCACTAGGATACCCTGTACCTGTTTCAGATAAAATTCCGGACATTACAGAAGCGAGGAACATTGAGCTCGGACTTGGGCTGCAGGTAGTTCTCCTCCCTCAAAGACATTACTTTTCTTTCCCCTCATTCGGAACCACATAATGATCATAGTACTTACATTCTCGATTTCGGTTTTTATTGTCTGATACAATGTTGCCATGTTTATCAAAGCTGATGTCTGATAATGACCTTAATTACATCTTAGGTGGGAAGGAGGAAAATTCAAACTTCTGTGTAATCTATAACTTCTGCTTGATGTTTGATGTATATCTAATGGGTTTTAGTTAGCGATGTAAATTCTGTTAACCAGCATGATCAAGAATTATCTCAGTCAATTTCTTAAATGAAATGCAGCTCTGTTTTTTGCACCCATCAAAACACAAGTTTGAGCATCCTCGGTTCTGCTACGAACGATTAGAATACCTCGGCCAAAAGATCCAGGTAAGACCTTTCTTACATGTCTTAGGAGGAAACACTCCTTCGACTTTTTGTAAATCCCGTCTACTTTTCACCCTTTATGTTTGGATCGAGAAATTTATGGGGTCAATCCAACTGTGTTCATTCAAAGGATATCGTCATGGCTGAGAGACTGTTGATGAAGCATTTAGATGCTCCCGGAAAGTGGTTGCAGGAGAAGCACCGGCGATTGCTGATGAACAAGTTCTGCGGGCGGTATTTGAGGGAGAAGTATCTTCATAAACTCATCATCTATTCCGAAAAAGTTCAAGATGCGTACGAGAACAATCGGAGGCTTCGAAACCCAGCTACCACGGCTGTTCAGCAAGCTCTTCATGGACTTTCTTATGCAGTTTATGGAAAGCCTGATGTTAGACGTCTCATGTTTGAGGTTTTTGACTTTGAGCAAATCCAACCTAAAGCCGTC

mRNA sequence

ATGGAACTTTCTTCTTCTTTTGTACAACTCGCATCCCCTACTCCTAATTACTCTTTCTCGTCTTCCTTAACCCCATTTCATTTCAAAATTTACCCCAATAATCCCAGTTTCCCGAACCCCACGTCGGGAAATTTGCGTATTTTTGCCGTTGCAGTTGACCCACAGCAAGAACTGCCCAAAAACAACCCCCAGAGACTGCTCAAGGAGCTCGCGGAGCGAAAACGAGCTACTTCTCCGAAGAAGAGGGTGCCCCCAAGAAGGTTTATTCTTAGACCGCCATTAGATGATAAGAAGTTAGCGGAAAGGTTTCTCAATAGCCCTCAATTAACCCTCAAATCATTCCCATTATTGAGTTCTTGTTTGCCATCTTCGCGGCTTAACAATGCCGATAAAACTTGGATGGACGAGTACTTGCTCGAAGCCAAGCAGGCTCTTGGATACCCCCTCGAGCCATCCGATAATTATGGAGACGACAATCCTGCGAAACAGTTTGACACTTTGTTGTACTTGGCCTTTCAGCATCCCTCGTGCGAGAGAACAAAGGCACGGCACATAAGATCGGGGCACTCAAGGCTACTGTTTTTGGGCCAATATGTGCTCGAATTGGCGTTAGCAGAGTATTTTCTGCAGAGATATCCTAGGGAATCGCCGGGTCCAATGAGGGAGCGAGTTTTTGCGTTAATTGGGAAGAGGAGCCTCCCAAAGTGGATTAAAGCTGCCAGTTTACAGAATTTGATATTCCCATACGATGATATGGATAAGATGATCAGGAAGGACCGTGAACCACCTGTGAAATCTGTGTTCTGGGCGTTGTTTGGGGCAATATACTTATGTTTTGGAATGCCAGAAGTGTATCGGGTCCTTTTTGAGGTGTTTGGAATGGATCCAGATGCAGAGGAATGTGAACCGAGGTTACGTAGGCAACTTGAAGATGTAGATTATGTCTCTGTTGAATTTGAGGACAAAAAGATCAGCTGGCAAGATATGGTTGCCTATAAGGCAAGTTTCATGTTTATTCTTAATAGCACACCTCCCGAAGATGCTCTATTTGCACACCCTAGGCTCTTCAGAGCTTGTGTCCCACCTGGCATGCACAGATTCCGGGGAAATATATGGGATTACGATAGCCGACCACAAGTCATGCAAGCACTAGGATACCCTGTACCTGTTTCAGATAAAATTCCGGACATTACAGAAGCGAGGAACATTGAGCTCGGACTTGGGCTGCAGCTCTGTTTTTTGCACCCATCAAAACACAAGTTTGAGCATCCTCGGTTCTGCTACGAACGATTAGAATACCTCGGCCAAAAGATCCAGGATATCGTCATGGCTGAGAGACTGTTGATGAAGCATTTAGATGCTCCCGGAAAGTGGTTGCAGGAGAAGCACCGGCGATTGCTGATGAACAAGTTCTGCGGGCGGTATTTGAGGGAGAAGTATCTTCATAAACTCATCATCTATTCCGAAAAAGTTCAAGATGCGTACGAGAACAATCGGAGGCTTCGAAACCCAGCTACCACGGCTGTTCAGCAAGCTCTTCATGGACTTTCTTATGCAGTTTATGGAAAGCCTGATGTTAGACGTCTCATGTTTGAGGTTTTTGACTTTGAGCAAATCCAACCTAAAGCCGTC

Coding sequence (CDS)

ATGGAACTTTCTTCTTCTTTTGTACAACTCGCATCCCCTACTCCTAATTACTCTTTCTCGTCTTCCTTAACCCCATTTCATTTCAAAATTTACCCCAATAATCCCAGTTTCCCGAACCCCACGTCGGGAAATTTGCGTATTTTTGCCGTTGCAGTTGACCCACAGCAAGAACTGCCCAAAAACAACCCCCAGAGACTGCTCAAGGAGCTCGCGGAGCGAAAACGAGCTACTTCTCCGAAGAAGAGGGTGCCCCCAAGAAGGTTTATTCTTAGACCGCCATTAGATGATAAGAAGTTAGCGGAAAGGTTTCTCAATAGCCCTCAATTAACCCTCAAATCATTCCCATTATTGAGTTCTTGTTTGCCATCTTCGCGGCTTAACAATGCCGATAAAACTTGGATGGACGAGTACTTGCTCGAAGCCAAGCAGGCTCTTGGATACCCCCTCGAGCCATCCGATAATTATGGAGACGACAATCCTGCGAAACAGTTTGACACTTTGTTGTACTTGGCCTTTCAGCATCCCTCGTGCGAGAGAACAAAGGCACGGCACATAAGATCGGGGCACTCAAGGCTACTGTTTTTGGGCCAATATGTGCTCGAATTGGCGTTAGCAGAGTATTTTCTGCAGAGATATCCTAGGGAATCGCCGGGTCCAATGAGGGAGCGAGTTTTTGCGTTAATTGGGAAGAGGAGCCTCCCAAAGTGGATTAAAGCTGCCAGTTTACAGAATTTGATATTCCCATACGATGATATGGATAAGATGATCAGGAAGGACCGTGAACCACCTGTGAAATCTGTGTTCTGGGCGTTGTTTGGGGCAATATACTTATGTTTTGGAATGCCAGAAGTGTATCGGGTCCTTTTTGAGGTGTTTGGAATGGATCCAGATGCAGAGGAATGTGAACCGAGGTTACGTAGGCAACTTGAAGATGTAGATTATGTCTCTGTTGAATTTGAGGACAAAAAGATCAGCTGGCAAGATATGGTTGCCTATAAGGCAAGTTTCATGTTTATTCTTAATAGCACACCTCCCGAAGATGCTCTATTTGCACACCCTAGGCTCTTCAGAGCTTGTGTCCCACCTGGCATGCACAGATTCCGGGGAAATATATGGGATTACGATAGCCGACCACAAGTCATGCAAGCACTAGGATACCCTGTACCTGTTTCAGATAAAATTCCGGACATTACAGAAGCGAGGAACATTGAGCTCGGACTTGGGCTGCAGCTCTGTTTTTTGCACCCATCAAAACACAAGTTTGAGCATCCTCGGTTCTGCTACGAACGATTAGAATACCTCGGCCAAAAGATCCAGGATATCGTCATGGCTGAGAGACTGTTGATGAAGCATTTAGATGCTCCCGGAAAGTGGTTGCAGGAGAAGCACCGGCGATTGCTGATGAACAAGTTCTGCGGGCGGTATTTGAGGGAGAAGTATCTTCATAAACTCATCATCTATTCCGAAAAAGTTCAAGATGCGTACGAGAACAATCGGAGGCTTCGAAACCCAGCTACCACGGCTGTTCAGCAAGCTCTTCATGGACTTTCTTATGCAGTTTATGGAAAGCCTGATGTTAGACGTCTCATGTTTGAGGTTTTTGACTTTGAGCAAATCCAACCTAAAGCCGTC

Protein sequence

MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPKNNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSCLPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERTKARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAASLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEECEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACVPPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHKFEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKYLHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQPKAV
Homology
BLAST of MS011484 vs. NCBI nr
Match: XP_022133289.1 (ribonuclease III domain-containing protein RNC1, chloroplastic [Momordica charantia])

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 534/544 (98.16%), Postives = 534/544 (98.16%), Query Frame = 0

Query: 1   MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK 60
           MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK
Sbjct: 1   MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK 60

Query: 61  NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120
           NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC
Sbjct: 61  NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120

Query: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT 180
           LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT
Sbjct: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT 180

Query: 181 KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA 240
           KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA
Sbjct: 181 KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA 240

Query: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300
           SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE
Sbjct: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300

Query: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACV 360
           CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYK          PPEDALFAHPRLFRACV
Sbjct: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYK----------PPEDALFAHPRLFRACV 360

Query: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK 420
           PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK
Sbjct: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK 420

Query: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480
           FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY
Sbjct: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480

Query: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ 540
           LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ
Sbjct: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ 534

Query: 541 PKAV 545
           PKAV
Sbjct: 541 PKAV 534

BLAST of MS011484 vs. NCBI nr
Match: XP_038883471.1 (ribonuclease III domain-containing protein RNC1, chloroplastic [Benincasa hispida])

HSP 1 Score: 1058.1 bits (2735), Expect = 2.5e-305
Identity = 514/544 (94.49%), Postives = 529/544 (97.24%), Query Frame = 0

Query: 1   MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK 60
           MELSSSFV+L+SP+PNYSFSSSLTPF  KI+PN+P FPNP+S NLRIFAVAVDPQQELPK
Sbjct: 1   MELSSSFVKLSSPSPNYSFSSSLTPFIVKIHPNHPIFPNPSSANLRIFAVAVDPQQELPK 60

Query: 61  NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120
           NNPQRLLKELAERKRATSPKK+VPP+RFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC
Sbjct: 61  NNPQRLLKELAERKRATSPKKKVPPKRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120

Query: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT 180
           LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSD+YGDDNPAKQFDTLLYLAFQHPSCERT
Sbjct: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDSYGDDNPAKQFDTLLYLAFQHPSCERT 180

Query: 181 KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA 240
           KARHIRSGHSRLLFLGQYV+ELALAEYFLQRYPRESPGPMRERVFALIGKR+LPKWIKAA
Sbjct: 181 KARHIRSGHSRLLFLGQYVIELALAEYFLQRYPRESPGPMRERVFALIGKRNLPKWIKAA 240

Query: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300
           SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE
Sbjct: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300

Query: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACV 360
           CEPRLRRQLEDVDYVSVEFEDK+ISWQDMV+YK          PPEDALFAHPRLFRACV
Sbjct: 301 CEPRLRRQLEDVDYVSVEFEDKRISWQDMVSYK----------PPEDALFAHPRLFRACV 360

Query: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK 420
           PPGMHRFRGNIWDYDSRPQVMQALGYP+PV+DKIPDITEARNIELGLGLQLCFLHPSKHK
Sbjct: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPLPVTDKIPDITEARNIELGLGLQLCFLHPSKHK 420

Query: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480
           FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY
Sbjct: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480

Query: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ 540
           LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSY VYGKPDVRRLMFEVFDFEQIQ
Sbjct: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYVVYGKPDVRRLMFEVFDFEQIQ 534

Query: 541 PKAV 545
           PKAV
Sbjct: 541 PKAV 534

BLAST of MS011484 vs. NCBI nr
Match: XP_008440359.1 (PREDICTED: ribonuclease III domain-containing protein RNC1, chloroplastic [Cucumis melo])

HSP 1 Score: 1056.2 bits (2730), Expect = 9.5e-305
Identity = 513/544 (94.30%), Postives = 528/544 (97.06%), Query Frame = 0

Query: 1   MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK 60
           MELSSSFV+L+SP+PNYSFSSSLTPF  KI+PN+P FPNP+SGNLRIFAVAVDPQQELPK
Sbjct: 1   MELSSSFVKLSSPSPNYSFSSSLTPFIVKIHPNHPIFPNPSSGNLRIFAVAVDPQQELPK 60

Query: 61  NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120
           NNPQRLLKELAERKRATSPKK+VPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC
Sbjct: 61  NNPQRLLKELAERKRATSPKKKVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120

Query: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT 180
           LPSSRLNNADKTWMDEYLLEAKQALGYPL+PSD+YGDDNPAKQFDTLLYLAFQHPSCERT
Sbjct: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLDPSDSYGDDNPAKQFDTLLYLAFQHPSCERT 180

Query: 181 KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA 240
           KARHIRSGHSRLLFLGQYV+ELALAEYFL RYPRESPGPMRERVFALIGKR+LPKWIKAA
Sbjct: 181 KARHIRSGHSRLLFLGQYVIELALAEYFLLRYPRESPGPMRERVFALIGKRNLPKWIKAA 240

Query: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300
           SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE
Sbjct: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300

Query: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACV 360
           CEPRLRRQLEDVDYVSVEFEDK+ISWQDM++YK          PPEDALFAHPRLFRACV
Sbjct: 301 CEPRLRRQLEDVDYVSVEFEDKRISWQDMISYK----------PPEDALFAHPRLFRACV 360

Query: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK 420
           PPGMHRFRGNIWDYDSRPQVMQALGYP+PV DKIPDITEARNIELGLGLQLCFLHPSKHK
Sbjct: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPLPVVDKIPDITEARNIELGLGLQLCFLHPSKHK 420

Query: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480
           FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY
Sbjct: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480

Query: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ 540
           LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSY VYGKPDVRRLMFEVFDFEQIQ
Sbjct: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYVVYGKPDVRRLMFEVFDFEQIQ 534

Query: 541 PKAV 545
           PKAV
Sbjct: 541 PKAV 534

BLAST of MS011484 vs. NCBI nr
Match: XP_023543359.1 (ribonuclease III domain-containing protein RNC1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1052.7 bits (2721), Expect = 1.1e-303
Identity = 511/544 (93.93%), Postives = 527/544 (96.88%), Query Frame = 0

Query: 1   MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK 60
           MELSS FV+L+SPTPN+SFSSSLTPF  KI+PNNPS  NP+SG+LRIFAVAVDPQQELPK
Sbjct: 1   MELSSCFVKLSSPTPNFSFSSSLTPFVVKIHPNNPSLSNPSSGSLRIFAVAVDPQQELPK 60

Query: 61  NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120
           NNPQRLLKEL ERKRATSPKK+VPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC
Sbjct: 61  NNPQRLLKELEERKRATSPKKKVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120

Query: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT 180
           LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSD+YGDDNPAKQFDTLLYLAFQHPSCERT
Sbjct: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDSYGDDNPAKQFDTLLYLAFQHPSCERT 180

Query: 181 KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA 240
           KARHIRSGHSRLLFLGQYV+ELALAEYFLQRYPRESPGPMRERVFALIGKR+LP+WIKAA
Sbjct: 181 KARHIRSGHSRLLFLGQYVIELALAEYFLQRYPRESPGPMRERVFALIGKRNLPQWIKAA 240

Query: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300
           SLQNLIFPYDDMDKM+RKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE
Sbjct: 241 SLQNLIFPYDDMDKMVRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300

Query: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACV 360
           CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYK          PPEDALFAHPRLFRACV
Sbjct: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYK----------PPEDALFAHPRLFRACV 360

Query: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK 420
           PPGMHRFRGNIWDYDSRPQVMQALGYPVPV+DKIP+ITEARNIELGLGLQLCFLHPSKHK
Sbjct: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVTDKIPEITEARNIELGLGLQLCFLHPSKHK 420

Query: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480
           FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY
Sbjct: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480

Query: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ 540
           LHKLI+YSE+VQDAYENNRRLRNPATTAVQQALHGLSY VYGKPDVRRLMFEVFDFEQIQ
Sbjct: 481 LHKLIVYSEQVQDAYENNRRLRNPATTAVQQALHGLSYTVYGKPDVRRLMFEVFDFEQIQ 534

Query: 541 PKAV 545
           PKAV
Sbjct: 541 PKAV 534

BLAST of MS011484 vs. NCBI nr
Match: XP_022949934.1 (ribonuclease III domain-containing protein RNC1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.4e-303
Identity = 511/544 (93.93%), Postives = 526/544 (96.69%), Query Frame = 0

Query: 1   MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK 60
           MELSS FV+L+SPTPN+SFSSSLTPF  KI+PNNPS  NP+SG LRIFAVAVDPQQELPK
Sbjct: 1   MELSSCFVKLSSPTPNFSFSSSLTPFVVKIHPNNPSLSNPSSGGLRIFAVAVDPQQELPK 60

Query: 61  NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120
           NNPQRLLKEL ERKRATSPKK+VPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC
Sbjct: 61  NNPQRLLKELEERKRATSPKKKVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120

Query: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT 180
           LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSD+YGDDNPAKQFDTLLYLAFQHPSCERT
Sbjct: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDSYGDDNPAKQFDTLLYLAFQHPSCERT 180

Query: 181 KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA 240
           KARHIRSGHSRLLFLGQYV+ELALAEYFLQRYPRESPGPMRERVFALIGKR+LP+WIKAA
Sbjct: 181 KARHIRSGHSRLLFLGQYVIELALAEYFLQRYPRESPGPMRERVFALIGKRNLPQWIKAA 240

Query: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300
           SLQNLIFPYDDMDKM+RKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE
Sbjct: 241 SLQNLIFPYDDMDKMVRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300

Query: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACV 360
           CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYK          PPEDALFAHPRLFRACV
Sbjct: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYK----------PPEDALFAHPRLFRACV 360

Query: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK 420
           PPGMHRFRGNIWDYDSRPQVMQALGYPVPV+DKIP+ITEARNIELGLGLQLCFLHPSKHK
Sbjct: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVTDKIPEITEARNIELGLGLQLCFLHPSKHK 420

Query: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480
           FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY
Sbjct: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480

Query: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ 540
           LHKLI+YSE+VQDAYENNRRLRNPATTAVQQALHGLSY VYGKPDVRRLMFEVFDFEQIQ
Sbjct: 481 LHKLIVYSEQVQDAYENNRRLRNPATTAVQQALHGLSYTVYGKPDVRRLMFEVFDFEQIQ 534

Query: 541 PKAV 545
           PKAV
Sbjct: 541 PKAV 534

BLAST of MS011484 vs. ExPASy Swiss-Prot
Match: Q9SZV0 (Ribonuclease III domain-containing protein RNC1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RNC1 PE=2 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 1.5e-257
Identity = 432/542 (79.70%), Postives = 476/542 (87.82%), Query Frame = 0

Query: 4   SSSFVQL-ASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPKNN 63
           SSS +++ +S  P  SFSSS++ F  K              NLRI A      Q LP+++
Sbjct: 9   SSSLLRICSSSAPEISFSSSISQFPSK---TQSILTKSRFQNLRICASVTAETQGLPRDS 68

Query: 64  PQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSCLP 123
           PQRLLKELA+RK AT PKK+VPP+RFILRPPLDDKKLAERFLNSPQL+LKSFPLLSSCLP
Sbjct: 69  PQRLLKELAQRKTATGPKKKVPPKRFILRPPLDDKKLAERFLNSPQLSLKSFPLLSSCLP 128

Query: 124 SSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERTKA 183
           SS+LNNADKTW+DEYLLE KQALGY LEPS++ GDDNPAK FDTLLYLAFQHPSC+R +A
Sbjct: 129 SSKLNNADKTWIDEYLLEVKQALGYSLEPSESLGDDNPAKHFDTLLYLAFQHPSCDRARA 188

Query: 184 RHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAASL 243
           RH+++GHSRL FLGQYVLELAL E+FLQRYPRESPGPMRERVFALIGKR LPKWIKAASL
Sbjct: 189 RHVKNGHSRLWFLGQYVLELALTEFFLQRYPRESPGPMRERVFALIGKRYLPKWIKAASL 248

Query: 244 QNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEECE 303
           QNLIFPYDDMDK+IRK+REPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDA+EC+
Sbjct: 249 QNLIFPYDDMDKLIRKEREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDADECQ 308

Query: 304 PRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACVPP 363
           PR RRQLEDVDYVSVEFE KK+ WQD+  YK          PPEDALFAHPRLFRACVPP
Sbjct: 309 PRSRRQLEDVDYVSVEFEGKKLGWQDIATYK----------PPEDALFAHPRLFRACVPP 368

Query: 364 GMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHKFE 423
           GMHRFRGNIWD+DS+P+VMQ LGYP+ ++D+I +ITEARNIELGLGLQLCFLHPSKHKFE
Sbjct: 369 GMHRFRGNIWDFDSKPKVMQTLGYPLTMNDRIKEITEARNIELGLGLQLCFLHPSKHKFE 428

Query: 424 HPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKYLH 483
           HPRFC+ERLEY+GQKIQDI MAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREK LH
Sbjct: 429 HPRFCFERLEYVGQKIQDIAMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKRLH 488

Query: 484 KLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQPK 543
             IIYSE+V D YE+NRRLRNPATTAVQQA+HGL+Y +YGKPDVRRLMFEVFDFEQIQPK
Sbjct: 489 NFIIYSEEVHDRYEHNRRLRNPATTAVQQAIHGLAYTIYGKPDVRRLMFEVFDFEQIQPK 537

Query: 544 AV 545
           AV
Sbjct: 549 AV 537

BLAST of MS011484 vs. ExPASy Swiss-Prot
Match: Q8S1Z0 (Ribonuclease III domain-containing protein RNC1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RNC1 PE=2 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 3.0e-237
Identity = 390/515 (75.73%), Postives = 446/515 (86.60%), Query Frame = 0

Query: 45  LRIFAVAVD---------PQQELPKNNPQRLLKELAERKRATSPKKRVPPRRFILRPPLD 104
           +R+ AVA D              P N+P RLL+ELAERK+A SPKK+ PPRRFIL+PPLD
Sbjct: 29  VRVLAVAADHTPPPPPSPSSPPEPANSPSRLLRELAERKKAVSPKKKHPPRRFILKPPLD 88

Query: 105 DKKLAERFLNSPQLTLKSFPLLSSCLPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNY 164
           D++L +RFL+SPQL+LK+ PLLSSCLPS+ L+ AD+TWMDEYLLEAKQALGYPL PS+  
Sbjct: 89  DERLTQRFLSSPQLSLKALPLLSSCLPSAPLSAADRTWMDEYLLEAKQALGYPLAPSETL 148

Query: 165 GDDN----PAKQFDTLLYLAFQH--PSCERTKARHIRSGHSRLLFLGQYVLELALAEYFL 224
           GD +    PA+ FD LLYLAFQH   SCERT+ RH+RSGHSRL FLGQYVLELA  E+FL
Sbjct: 149 GDGDDDGCPARHFDVLLYLAFQHLDTSCERTRTRHVRSGHSRLWFLGQYVLELAFCEFFL 208

Query: 225 QRYPRESPGPMRERVFALIGKRSLPKWIKAASLQNLIFPYDDMDKMIRKDREPPVKSVFW 284
           QRYPRESPGPMRERVFALIGKR++PKWIKAASL NL+FPYDD+DKMIRKDREPP K+VFW
Sbjct: 209 QRYPRESPGPMRERVFALIGKRAIPKWIKAASLHNLVFPYDDLDKMIRKDREPPAKAVFW 268

Query: 285 ALFGAIYLCFGMPEVYRVLFEVFGMDPDAEECEPRLRRQLEDVDYVSVEFEDKKISWQDM 344
           ALFGAIYLCFGMPEVYRVLFE FGMDP+ E C+P+LRRQLEDVDYVSVEFE ++++WQD+
Sbjct: 269 ALFGAIYLCFGMPEVYRVLFEAFGMDPEDESCQPKLRRQLEDVDYVSVEFEKRQLTWQDV 328

Query: 345 VAYKASFMFILNSTPPEDALFAHPRLFRACVPPGMHRFRGNIWDYDSRPQVMQALGYPVP 404
            AY+          PP DALFAHPRLFRACVPPGMHRFRGNIWD+D+RP+VM  LGYP+P
Sbjct: 329 AAYR----------PPPDALFAHPRLFRACVPPGMHRFRGNIWDFDNRPKVMNTLGYPLP 388

Query: 405 VSDKIPDITEARNIELGLGLQLCFLHPSKHKFEHPRFCYERLEYLGQKIQDIVMAERLLM 464
           ++D+IP+ITEARNIELGLGLQLCFLHPSKHKFEHPRFC ERLEY+GQKIQD+VMAERLLM
Sbjct: 389 MNDRIPEITEARNIELGLGLQLCFLHPSKHKFEHPRFCLERLEYVGQKIQDLVMAERLLM 448

Query: 465 KHLDAPGKWLQEKHRRLLMNKFCGRYLREKYLHKLIIYSEKVQDAYENNRRLRNPATTAV 524
           KHLDAPG+WL EKHRRLLMNK+CGRYLR+K+LH  IIY E VQD +E+NRRLRNP+TTAV
Sbjct: 449 KHLDAPGRWLAEKHRRLLMNKYCGRYLRDKHLHHYIIYGESVQDRFEHNRRLRNPSTTAV 508

Query: 525 QQALHGLSYAVYGKPDVRRLMFEVFDFEQIQPKAV 545
           QQA+HGL+Y VYGKPDVRRLMFEVFDFEQ+QPKAV
Sbjct: 509 QQAIHGLAYCVYGKPDVRRLMFEVFDFEQVQPKAV 533

BLAST of MS011484 vs. ExPASy Swiss-Prot
Match: A6YSL1 (Ribonuclease III domain-containing protein RNC1, chloroplastic OS=Zea mays OX=4577 GN=RNC1 PE=1 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 2.6e-236
Identity = 393/533 (73.73%), Postives = 453/533 (84.99%), Query Frame = 0

Query: 22  SLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVD------PQQELPKNNPQRLLKELAERKR 81
           +LTP  F ++         +S  +R+ AVA D      P  E P N+P RLL+ELA+RK+
Sbjct: 12  TLTPLPFSLH--------SSSRRVRVLAVAADQTPPPAPPSE-PANSPSRLLRELAQRKK 71

Query: 82  ATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSCLPSSRLNNADKTWMD 141
           A SPKK+ PPRRFIL+PPLDD++L  RFL+SPQL+LK+ PLLSSCLPS+ L+ AD+TWMD
Sbjct: 72  AVSPKKKHPPRRFILKPPLDDERLTRRFLSSPQLSLKALPLLSSCLPSAPLSTADRTWMD 131

Query: 142 EYLLEAKQALGYPLEPSDNY--GDDNPAKQFDTLLYLAFQH--PSCERTKARHIRSGHSR 201
           EYLLEAKQALGYPL PS+    GDD PA+ FD L YLAFQH  PS ERT+ RH+R+GHSR
Sbjct: 132 EYLLEAKQALGYPLAPSETLGEGDDCPARHFDVLFYLAFQHLDPSSERTRMRHVRNGHSR 191

Query: 202 LLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAASLQNLIFPYDD 261
           L FLGQYVLELA  E+FLQRYPRESPGPMRERVFALIGK+ LP+W+KAASL NL+FPYDD
Sbjct: 192 LWFLGQYVLELAFCEFFLQRYPRESPGPMRERVFALIGKKVLPRWLKAASLHNLVFPYDD 251

Query: 262 MDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEECEPRLRRQLED 321
           +DKMIRKDREPP K+VFWA+FGAIYLCFGMPEVYRVLFE FGMDPD E C+P+LRRQLED
Sbjct: 252 LDKMIRKDREPPSKAVFWAIFGAIYLCFGMPEVYRVLFEAFGMDPDDESCQPKLRRQLED 311

Query: 322 VDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACVPPGMHRFRGNI 381
           VDYVSVEFE ++++WQD+ AY+          PP DALFAHPRLFRACVPPGMHRFRGNI
Sbjct: 312 VDYVSVEFEKRQLTWQDVAAYR----------PPPDALFAHPRLFRACVPPGMHRFRGNI 371

Query: 382 WDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHKFEHPRFCYERL 441
           WD+DSRP+VM  LGYP+P++D+IP+ITEARNIELGLGLQLCFLHPSKHKFEHPRFCYERL
Sbjct: 372 WDFDSRPKVMTTLGYPLPMNDRIPEITEARNIELGLGLQLCFLHPSKHKFEHPRFCYERL 431

Query: 442 EYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKYLHKLIIYSEKV 501
           EY+GQKIQD+VMAERLLMKHLDAPG+WL EKHRR LMNK+CGRYLR+K+L   IIY E V
Sbjct: 432 EYVGQKIQDLVMAERLLMKHLDAPGRWLAEKHRRTLMNKYCGRYLRDKHLQHYIIYGETV 491

Query: 502 QDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQPKAV 545
           QD +E+NRRLRNP+TT+VQQALHGL+Y VYGKPDVRRLMFEVFDFEQ+QPKAV
Sbjct: 492 QDRFEHNRRLRNPSTTSVQQALHGLAYCVYGKPDVRRLMFEVFDFEQVQPKAV 525

BLAST of MS011484 vs. ExPASy Swiss-Prot
Match: Q5L0Q3 (Ribonuclease 3 OS=Geobacillus kaustophilus (strain HTA426) OX=235909 GN=rnc PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 5.7e-10
Identity = 41/124 (33.06%), Postives = 60/124 (48.39%), Query Frame = 0

Query: 165 DTLLYLAFQHPSCERTKARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERV 224
           + LL  AF H S      R +   + RL FLG  VLEL +++Y  Q++P  S G + +  
Sbjct: 32  EKLLIQAFTHSSYVNEHRRRLHEDNERLEFLGDAVLELTVSQYLFQKFPHMSEGQLTKLR 91

Query: 225 FALIGKRSLPKWIKAASLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEV 284
            A++ + SL K+  A S   L+      +    + R   +  VF A  GA+YL  GM  V
Sbjct: 92  AAIVCEPSLVKFANALSFGELVLLGKGEELTGGRTRPALLADVFEAFIGALYLDQGMDAV 151

Query: 285 YRVL 289
            R L
Sbjct: 152 IRFL 155

BLAST of MS011484 vs. ExPASy Swiss-Prot
Match: A4IM67 (Ribonuclease 3 OS=Geobacillus thermodenitrificans (strain NG80-2) OX=420246 GN=rnc PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 7.5e-10
Identity = 42/126 (33.33%), Postives = 60/126 (47.62%), Query Frame = 0

Query: 163 QFDTLLYLAFQHPSCERTKARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRE 222
           Q + LL  AF H S      R     + RL FLG  VLEL +++Y  Q++P  S G + +
Sbjct: 30  QNEKLLIQAFTHSSYVNEHRRRFHEDNERLEFLGDAVLELTVSQYLFQKFPHMSEGQLTK 89

Query: 223 RVFALIGKRSLPKWIKAASLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMP 282
              A++ + SL K+  A S   L+      +    + R   +  VF A  GA+YL  GM 
Sbjct: 90  LRAAIVCEPSLVKFANALSFGELVLLGKGEELTGGRTRPALLADVFEAFIGALYLDQGMD 149

Query: 283 EVYRVL 289
            V R L
Sbjct: 150 VVLRFL 155

BLAST of MS011484 vs. ExPASy TrEMBL
Match: A0A6J1BVK6 (ribonuclease III domain-containing protein RNC1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111005910 PE=4 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 534/544 (98.16%), Postives = 534/544 (98.16%), Query Frame = 0

Query: 1   MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK 60
           MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK
Sbjct: 1   MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK 60

Query: 61  NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120
           NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC
Sbjct: 61  NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120

Query: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT 180
           LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT
Sbjct: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT 180

Query: 181 KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA 240
           KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA
Sbjct: 181 KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA 240

Query: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300
           SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE
Sbjct: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300

Query: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACV 360
           CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYK          PPEDALFAHPRLFRACV
Sbjct: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYK----------PPEDALFAHPRLFRACV 360

Query: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK 420
           PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK
Sbjct: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK 420

Query: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480
           FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY
Sbjct: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480

Query: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ 540
           LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ
Sbjct: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ 534

Query: 541 PKAV 545
           PKAV
Sbjct: 541 PKAV 534

BLAST of MS011484 vs. ExPASy TrEMBL
Match: A0A1S3B1J9 (ribonuclease III domain-containing protein RNC1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103484834 PE=4 SV=1)

HSP 1 Score: 1056.2 bits (2730), Expect = 4.6e-305
Identity = 513/544 (94.30%), Postives = 528/544 (97.06%), Query Frame = 0

Query: 1   MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK 60
           MELSSSFV+L+SP+PNYSFSSSLTPF  KI+PN+P FPNP+SGNLRIFAVAVDPQQELPK
Sbjct: 1   MELSSSFVKLSSPSPNYSFSSSLTPFIVKIHPNHPIFPNPSSGNLRIFAVAVDPQQELPK 60

Query: 61  NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120
           NNPQRLLKELAERKRATSPKK+VPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC
Sbjct: 61  NNPQRLLKELAERKRATSPKKKVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120

Query: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT 180
           LPSSRLNNADKTWMDEYLLEAKQALGYPL+PSD+YGDDNPAKQFDTLLYLAFQHPSCERT
Sbjct: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLDPSDSYGDDNPAKQFDTLLYLAFQHPSCERT 180

Query: 181 KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA 240
           KARHIRSGHSRLLFLGQYV+ELALAEYFL RYPRESPGPMRERVFALIGKR+LPKWIKAA
Sbjct: 181 KARHIRSGHSRLLFLGQYVIELALAEYFLLRYPRESPGPMRERVFALIGKRNLPKWIKAA 240

Query: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300
           SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE
Sbjct: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300

Query: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACV 360
           CEPRLRRQLEDVDYVSVEFEDK+ISWQDM++YK          PPEDALFAHPRLFRACV
Sbjct: 301 CEPRLRRQLEDVDYVSVEFEDKRISWQDMISYK----------PPEDALFAHPRLFRACV 360

Query: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK 420
           PPGMHRFRGNIWDYDSRPQVMQALGYP+PV DKIPDITEARNIELGLGLQLCFLHPSKHK
Sbjct: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPLPVVDKIPDITEARNIELGLGLQLCFLHPSKHK 420

Query: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480
           FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY
Sbjct: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480

Query: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ 540
           LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSY VYGKPDVRRLMFEVFDFEQIQ
Sbjct: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYVVYGKPDVRRLMFEVFDFEQIQ 534

Query: 541 PKAV 545
           PKAV
Sbjct: 541 PKAV 534

BLAST of MS011484 vs. ExPASy TrEMBL
Match: A0A6J1GEC3 (ribonuclease III domain-containing protein RNC1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111453180 PE=4 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 6.7e-304
Identity = 511/544 (93.93%), Postives = 526/544 (96.69%), Query Frame = 0

Query: 1   MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK 60
           MELSS FV+L+SPTPN+SFSSSLTPF  KI+PNNPS  NP+SG LRIFAVAVDPQQELPK
Sbjct: 1   MELSSCFVKLSSPTPNFSFSSSLTPFVVKIHPNNPSLSNPSSGGLRIFAVAVDPQQELPK 60

Query: 61  NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120
           NNPQRLLKEL ERKRATSPKK+VPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC
Sbjct: 61  NNPQRLLKELEERKRATSPKKKVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120

Query: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT 180
           LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSD+YGDDNPAKQFDTLLYLAFQHPSCERT
Sbjct: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDSYGDDNPAKQFDTLLYLAFQHPSCERT 180

Query: 181 KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA 240
           KARHIRSGHSRLLFLGQYV+ELALAEYFLQRYPRESPGPMRERVFALIGKR+LP+WIKAA
Sbjct: 181 KARHIRSGHSRLLFLGQYVIELALAEYFLQRYPRESPGPMRERVFALIGKRNLPQWIKAA 240

Query: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300
           SLQNLIFPYDDMDKM+RKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE
Sbjct: 241 SLQNLIFPYDDMDKMVRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300

Query: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACV 360
           CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYK          PPEDALFAHPRLFRACV
Sbjct: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYK----------PPEDALFAHPRLFRACV 360

Query: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK 420
           PPGMHRFRGNIWDYDSRPQVMQALGYPVPV+DKIP+ITEARNIELGLGLQLCFLHPSKHK
Sbjct: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVTDKIPEITEARNIELGLGLQLCFLHPSKHK 420

Query: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480
           FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY
Sbjct: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480

Query: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ 540
           LHKLI+YSE+VQDAYENNRRLRNPATTAVQQALHGLSY VYGKPDVRRLMFEVFDFEQIQ
Sbjct: 481 LHKLIVYSEQVQDAYENNRRLRNPATTAVQQALHGLSYTVYGKPDVRRLMFEVFDFEQIQ 534

Query: 541 PKAV 545
           PKAV
Sbjct: 541 PKAV 534

BLAST of MS011484 vs. ExPASy TrEMBL
Match: A0A6J1IRK8 (ribonuclease III domain-containing protein RNC1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477828 PE=4 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 4.8e-302
Identity = 507/544 (93.20%), Postives = 526/544 (96.69%), Query Frame = 0

Query: 1   MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK 60
           MELSS FV+L+SPTPN+SFSSSLTPF  KI+PNNPS  NP+SG+LRIFAVAVDP+QELPK
Sbjct: 1   MELSSCFVKLSSPTPNFSFSSSLTPFVVKIHPNNPSLSNPSSGSLRIFAVAVDPRQELPK 60

Query: 61  NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120
           NNPQRLLKEL ERKRATSPKK+VPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC
Sbjct: 61  NNPQRLLKELEERKRATSPKKKVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120

Query: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT 180
           LPSSRLNNADKTWM+EYLLEAKQALGYPLEP D+YGDDNPAKQFDTLLYLAFQHPSCERT
Sbjct: 121 LPSSRLNNADKTWMEEYLLEAKQALGYPLEPFDSYGDDNPAKQFDTLLYLAFQHPSCERT 180

Query: 181 KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA 240
           KARHIRSGHSRLLFLGQYV+ELALAEYFLQRYPRESPGPMRERVFALIGKR+LP+WIKAA
Sbjct: 181 KARHIRSGHSRLLFLGQYVIELALAEYFLQRYPRESPGPMRERVFALIGKRNLPQWIKAA 240

Query: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300
           SLQNLIFPYDDMDKM+RKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE
Sbjct: 241 SLQNLIFPYDDMDKMVRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300

Query: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACV 360
           CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYK          PPEDALFAHPRLFRACV
Sbjct: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYK----------PPEDALFAHPRLFRACV 360

Query: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK 420
           PPGMHRFRGNIWDYDSRPQVMQALGYPVPV+DKIP+ITEARNIELGLGLQLCFLHPSKHK
Sbjct: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVTDKIPEITEARNIELGLGLQLCFLHPSKHK 420

Query: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480
           FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY
Sbjct: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480

Query: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ 540
           LHKLI+YSE+VQDAYENNRRLRNPATTAVQQ+LHGLSY VYGKPDVRRLMFEVFDFEQIQ
Sbjct: 481 LHKLIVYSEQVQDAYENNRRLRNPATTAVQQSLHGLSYTVYGKPDVRRLMFEVFDFEQIQ 534

Query: 541 PKAV 545
           PKAV
Sbjct: 541 PKAV 534

BLAST of MS011484 vs. ExPASy TrEMBL
Match: A0A0A0KGG7 (RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G494980 PE=4 SV=1)

HSP 1 Score: 1039.3 bits (2686), Expect = 5.8e-300
Identity = 505/544 (92.83%), Postives = 525/544 (96.51%), Query Frame = 0

Query: 1   MELSSSFVQLASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPK 60
           MELSSSFV+L+SP+ NYSFSSSLTPF  KI+PN+P FPNP+S NLR+FAVAVDPQQELPK
Sbjct: 1   MELSSSFVKLSSPS-NYSFSSSLTPFIVKIHPNHPIFPNPSSANLRVFAVAVDPQQELPK 60

Query: 61  NNPQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSC 120
           NNPQRLLKELAERKRATSPKK+VPPRRFILRPPLDDK+LAERFLNSPQLTLKSFPLLSSC
Sbjct: 61  NNPQRLLKELAERKRATSPKKKVPPRRFILRPPLDDKRLAERFLNSPQLTLKSFPLLSSC 120

Query: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERT 180
           LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSD+YGDDNPAKQFDTLLYLAFQHPSCERT
Sbjct: 121 LPSSRLNNADKTWMDEYLLEAKQALGYPLEPSDSYGDDNPAKQFDTLLYLAFQHPSCERT 180

Query: 181 KARHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAA 240
           KARHIRSGHSRL FLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKR+LPKWIKAA
Sbjct: 181 KARHIRSGHSRLFFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRNLPKWIKAA 240

Query: 241 SLQNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEE 300
           SLQNLIFPYDDMDK+IRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDA+E
Sbjct: 241 SLQNLIFPYDDMDKIIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAKE 300

Query: 301 CEPRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACV 360
           CEPRLRRQLEDVDYVSVEFEDK+I WQDMV+YK          PPEDALFAHPRLFRACV
Sbjct: 301 CEPRLRRQLEDVDYVSVEFEDKRIGWQDMVSYK----------PPEDALFAHPRLFRACV 360

Query: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHK 420
           PPGMHRFRGNIWDYDSRPQVMQALGYP+PV+DKIPDITEARNIELGLGLQLCFLHPSKHK
Sbjct: 361 PPGMHRFRGNIWDYDSRPQVMQALGYPLPVTDKIPDITEARNIELGLGLQLCFLHPSKHK 420

Query: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKY 480
           FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPG+WLQ KHRRLLMNKFCGRYLRE+Y
Sbjct: 421 FEHPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGRWLQVKHRRLLMNKFCGRYLRERY 480

Query: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQ 540
           LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSY VYGKPDVRR+MFEVFDFEQIQ
Sbjct: 481 LHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYVVYGKPDVRRIMFEVFDFEQIQ 533

Query: 541 PKAV 545
           PKAV
Sbjct: 541 PKAV 533

BLAST of MS011484 vs. TAIR 10
Match: AT4G37510.1 (Ribonuclease III family protein )

HSP 1 Score: 889.8 bits (2298), Expect = 1.1e-258
Identity = 432/542 (79.70%), Postives = 476/542 (87.82%), Query Frame = 0

Query: 4   SSSFVQL-ASPTPNYSFSSSLTPFHFKIYPNNPSFPNPTSGNLRIFAVAVDPQQELPKNN 63
           SSS +++ +S  P  SFSSS++ F  K              NLRI A      Q LP+++
Sbjct: 9   SSSLLRICSSSAPEISFSSSISQFPSK---TQSILTKSRFQNLRICASVTAETQGLPRDS 68

Query: 64  PQRLLKELAERKRATSPKKRVPPRRFILRPPLDDKKLAERFLNSPQLTLKSFPLLSSCLP 123
           PQRLLKELA+RK AT PKK+VPP+RFILRPPLDDKKLAERFLNSPQL+LKSFPLLSSCLP
Sbjct: 69  PQRLLKELAQRKTATGPKKKVPPKRFILRPPLDDKKLAERFLNSPQLSLKSFPLLSSCLP 128

Query: 124 SSRLNNADKTWMDEYLLEAKQALGYPLEPSDNYGDDNPAKQFDTLLYLAFQHPSCERTKA 183
           SS+LNNADKTW+DEYLLE KQALGY LEPS++ GDDNPAK FDTLLYLAFQHPSC+R +A
Sbjct: 129 SSKLNNADKTWIDEYLLEVKQALGYSLEPSESLGDDNPAKHFDTLLYLAFQHPSCDRARA 188

Query: 184 RHIRSGHSRLLFLGQYVLELALAEYFLQRYPRESPGPMRERVFALIGKRSLPKWIKAASL 243
           RH+++GHSRL FLGQYVLELAL E+FLQRYPRESPGPMRERVFALIGKR LPKWIKAASL
Sbjct: 189 RHVKNGHSRLWFLGQYVLELALTEFFLQRYPRESPGPMRERVFALIGKRYLPKWIKAASL 248

Query: 244 QNLIFPYDDMDKMIRKDREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDAEECE 303
           QNLIFPYDDMDK+IRK+REPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDA+EC+
Sbjct: 249 QNLIFPYDDMDKLIRKEREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDADECQ 308

Query: 304 PRLRRQLEDVDYVSVEFEDKKISWQDMVAYKASFMFILNSTPPEDALFAHPRLFRACVPP 363
           PR RRQLEDVDYVSVEFE KK+ WQD+  YK          PPEDALFAHPRLFRACVPP
Sbjct: 309 PRSRRQLEDVDYVSVEFEGKKLGWQDIATYK----------PPEDALFAHPRLFRACVPP 368

Query: 364 GMHRFRGNIWDYDSRPQVMQALGYPVPVSDKIPDITEARNIELGLGLQLCFLHPSKHKFE 423
           GMHRFRGNIWD+DS+P+VMQ LGYP+ ++D+I +ITEARNIELGLGLQLCFLHPSKHKFE
Sbjct: 369 GMHRFRGNIWDFDSKPKVMQTLGYPLTMNDRIKEITEARNIELGLGLQLCFLHPSKHKFE 428

Query: 424 HPRFCYERLEYLGQKIQDIVMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKYLH 483
           HPRFC+ERLEY+GQKIQDI MAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREK LH
Sbjct: 429 HPRFCFERLEYVGQKIQDIAMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKRLH 488

Query: 484 KLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQPK 543
             IIYSE+V D YE+NRRLRNPATTAVQQA+HGL+Y +YGKPDVRRLMFEVFDFEQIQPK
Sbjct: 489 NFIIYSEEVHDRYEHNRRLRNPATTAVQQAIHGLAYTIYGKPDVRRLMFEVFDFEQIQPK 537

Query: 544 AV 545
           AV
Sbjct: 549 AV 537

BLAST of MS011484 vs. TAIR 10
Match: AT2G48121.2 (LOCATED IN: mitochondrion; BEST Arabidopsis thaliana protein match is: Ribonuclease III family protein (TAIR:AT4G37510.1). )

HSP 1 Score: 141.4 bits (355), Expect = 2.2e-33
Identity = 66/77 (85.71%), Postives = 71/77 (92.21%), Query Frame = 0

Query: 468 MNKFCGRYLREKYLHKLIIYSEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVR 527
           MNKFCGRYLREK LH  IIYSE+V D YE+NRRLRNPATTAVQQA+HGL+Y +YGKPDVR
Sbjct: 1   MNKFCGRYLREKRLHNFIIYSEEVHDRYEHNRRLRNPATTAVQQAIHGLAYTIYGKPDVR 60

Query: 528 RLMFEVFDFEQIQPKAV 545
           RLMFEVFDFEQIQPKAV
Sbjct: 61  RLMFEVFDFEQIQPKAV 77

BLAST of MS011484 vs. TAIR 10
Match: AT2G48121.1 (BEST Arabidopsis thaliana protein match is: Ribonuclease III family protein (TAIR:AT4G37510.1). )

HSP 1 Score: 105.1 bits (261), Expect = 1.8e-22
Identity = 48/57 (84.21%), Postives = 54/57 (94.74%), Query Frame = 0

Query: 488 SEKVQDAYENNRRLRNPATTAVQQALHGLSYAVYGKPDVRRLMFEVFDFEQIQPKAV 545
           +E+V D YE+NRRLRNPATTAVQQA+HGL+Y +YGKPDVRRLMFEVFDFEQIQPKAV
Sbjct: 19  TEEVHDRYEHNRRLRNPATTAVQQAIHGLAYTIYGKPDVRRLMFEVFDFEQIQPKAV 75

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133289.10.0e+0098.16ribonuclease III domain-containing protein RNC1, chloroplastic [Momordica charan... [more]
XP_038883471.12.5e-30594.49ribonuclease III domain-containing protein RNC1, chloroplastic [Benincasa hispid... [more]
XP_008440359.19.5e-30594.30PREDICTED: ribonuclease III domain-containing protein RNC1, chloroplastic [Cucum... [more]
XP_023543359.11.1e-30393.93ribonuclease III domain-containing protein RNC1, chloroplastic-like [Cucurbita p... [more]
XP_022949934.11.4e-30393.93ribonuclease III domain-containing protein RNC1, chloroplastic-like [Cucurbita m... [more]
Match NameE-valueIdentityDescription
Q9SZV01.5e-25779.70Ribonuclease III domain-containing protein RNC1, chloroplastic OS=Arabidopsis th... [more]
Q8S1Z03.0e-23775.73Ribonuclease III domain-containing protein RNC1, chloroplastic OS=Oryza sativa s... [more]
A6YSL12.6e-23673.73Ribonuclease III domain-containing protein RNC1, chloroplastic OS=Zea mays OX=45... [more]
Q5L0Q35.7e-1033.06Ribonuclease 3 OS=Geobacillus kaustophilus (strain HTA426) OX=235909 GN=rnc PE=3... [more]
A4IM677.5e-1033.33Ribonuclease 3 OS=Geobacillus thermodenitrificans (strain NG80-2) OX=420246 GN=r... [more]
Match NameE-valueIdentityDescription
A0A6J1BVK60.0e+0098.16ribonuclease III domain-containing protein RNC1, chloroplastic OS=Momordica char... [more]
A0A1S3B1J94.6e-30594.30ribonuclease III domain-containing protein RNC1, chloroplastic OS=Cucumis melo O... [more]
A0A6J1GEC36.7e-30493.93ribonuclease III domain-containing protein RNC1, chloroplastic-like OS=Cucurbita... [more]
A0A6J1IRK84.8e-30293.20ribonuclease III domain-containing protein RNC1, chloroplastic-like OS=Cucurbita... [more]
A0A0A0KGG75.8e-30092.83RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G494980 P... [more]
Match NameE-valueIdentityDescription
AT4G37510.11.1e-25879.70Ribonuclease III family protein [more]
AT2G48121.22.2e-3385.71LOCATED IN: mitochondrion; BEST Arabidopsis thaliana protein match is: Ribonucle... [more]
AT2G48121.11.8e-2284.21BEST Arabidopsis thaliana protein match is: Ribonuclease III family protein (TAI... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000999Ribonuclease III domainSMARTSM00535riboneu5coord: 166..303
e-value: 7.3E-27
score: 105.2
IPR000999Ribonuclease III domainPFAMPF14622Ribonucleas_3_3coord: 166..287
e-value: 9.5E-9
score: 35.5
IPR000999Ribonuclease III domainPROSITEPS50142RNASE_3_2coord: 167..280
score: 15.471435
IPR000999Ribonuclease III domainCDDcd00593RIBOccoord: 166..297
e-value: 7.00246E-30
score: 111.936
IPR000999Ribonuclease III domainCDDcd00593RIBOccoord: 409..534
e-value: 7.43943E-20
score: 83.8162
IPR036389Ribonuclease III, endonuclease domain superfamilyGENE3D1.10.1520.10Ribonuclease III domaincoord: 163..294
e-value: 5.3E-15
score: 57.3
coord: 401..537
e-value: 4.5E-11
score: 44.5
IPR036389Ribonuclease III, endonuclease domain superfamilySUPERFAMILY69065RNase III domain-likecoord: 132..293
IPR036389Ribonuclease III, endonuclease domain superfamilySUPERFAMILY69065RNase III domain-likecoord: 409..535
NoneNo IPR availablePANTHERPTHR11207:SF21RIBONUCLEASE III DOMAIN-CONTAINING PROTEIN RNC1, CHLOROPLASTICcoord: 1..541
IPR011907Ribonuclease IIIPANTHERPTHR11207RIBONUCLEASE IIIcoord: 1..541

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS011484.1MS011484.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006396 RNA processing
biological_process GO:0016075 rRNA catabolic process
molecular_function GO:0004525 ribonuclease III activity
molecular_function GO:0003723 RNA binding