MS011437 (gene) Bitter gourd (TR) v1

Overview


Name	MS011437
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ent-kaur-16-ene synthase, chloroplastic
Location	scaffold239: 1929211 .. 1929312 (+)
RNA-Seq Expression	MS011437
Synteny	MS011437

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCTGCTTAAATTTTGGTCATCAAGATTTTCTGGAACTTGCTGTAGAAGATTTCAATATCCTGCAATCCATACATCATAAAGAACTGAAAGAGCTTGAAAGG

mRNA sequence

AGCTGCTTAAATTTTGGTCATCAAGATTTTCTGGAACTTGCTGTAGAAGATTTCAATATCCTGCAATCCATACATCATAAAGAACTGAAAGAGCTTGAAAGG

Coding sequence (CDS)

AGCTGCTTAAATTTTGGTCATCAAGATTTTCTGGAACTTGCTGTAGAAGATTTCAATATCCTGCAATCCATACATCATAAAGAACTGAAAGAGCTTGAAAGG

Protein sequence

SCLNFGHQDFLELAVEDFNILQSIHHKELKELER

Homology

[Close]

BLAST of MS011437 vs. NCBI nr
Match: XP_022147908.1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 71.6 bits (174), Expect = 1.4e-09
Identity = 33/34 (97.06%), Postives = 34/34 (100.00%), Query Frame = 0


Query: 1   SCLNFGHQDFLELAVEDFNILQSIHHKELKELER 35
           +CLNFGHQDFLELAVEDFNILQSIHHKELKELER
Sbjct: 497 TCLNFGHQDFLELAVEDFNILQSIHHKELKELER 530

[Close]

BLAST of MS011437 vs. NCBI nr
Match: XP_022147910.1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 [Momordica charantia])

HSP 1 Score: 71.6 bits (174), Expect = 1.4e-09
Identity = 33/34 (97.06%), Postives = 34/34 (100.00%), Query Frame = 0


Query: 1   SCLNFGHQDFLELAVEDFNILQSIHHKELKELER 35
           +CLNFGHQDFLELAVEDFNILQSIHHKELKELER
Sbjct: 484 TCLNFGHQDFLELAVEDFNILQSIHHKELKELER 517

[Close]

BLAST of MS011437 vs. NCBI nr
Match: XP_022147909.1 (ent-kaur-16-ene synthase, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 71.6 bits (174), Expect = 1.4e-09
Identity = 33/34 (97.06%), Postives = 34/34 (100.00%), Query Frame = 0


Query: 1   SCLNFGHQDFLELAVEDFNILQSIHHKELKELER 35
           +CLNFGHQDFLELAVEDFNILQSIHHKELKELER
Sbjct: 495 TCLNFGHQDFLELAVEDFNILQSIHHKELKELER 528

[Close]

BLAST of MS011437 vs. NCBI nr
Match: NP_001292675.1 (ent-kaur-16-ene synthase, chloroplastic [Cucumis sativus] >XP_004139844.2 ent-kaur-16-ene synthase, chloroplastic isoform X1 [Cucumis sativus] >BAB19275.1 ent-kaurene synthase [Cucumis sativus])

HSP 1 Score: 63.9 bits (154), Expect = 3.0e-07
Identity = 29/34 (85.29%), Postives = 31/34 (91.18%), Query Frame = 0


Query: 1   SCLNFGHQDFLELAVEDFNILQSIHHKELKELER 35
           +CLNFGHQDFLELAVEDFN LQ IH KELKELE+
Sbjct: 448 ACLNFGHQDFLELAVEDFNTLQGIHRKELKELEK 481

[Close]

BLAST of MS011437 vs. NCBI nr
Match: XP_011659051.1 (ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 63.9 bits (154), Expect = 3.0e-07
Identity = 29/34 (85.29%), Postives = 31/34 (91.18%), Query Frame = 0


Query: 1   SCLNFGHQDFLELAVEDFNILQSIHHKELKELER 35
           +CLNFGHQDFLELAVEDFN LQ IH KELKELE+
Sbjct: 384 ACLNFGHQDFLELAVEDFNTLQGIHRKELKELEK 417

[Close]

BLAST of MS011437 vs. ExPASy Swiss-Prot
Match: Q39548 (Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.3e-09
Identity = 28/34 (82.35%), Postives = 31/34 (91.18%), Query Frame = 0


Query: 1   SCLNFGHQDFLELAVEDFNILQSIHHKELKELER 35
           +CLNFGHQDFLELAVEDFN LQ IH KEL+EL+R
Sbjct: 452 ACLNFGHQDFLELAVEDFNTLQRIHLKELEELQR 485

[Close]

BLAST of MS011437 vs. ExPASy Swiss-Prot
Match: A0A3G1QTS7 (Ent-kaurene synthase 1, chloroplastic OS=Isodon eriocalyx OX=662907 GN=KS1 PE=1 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 8.5e-05
Identity = 21/31 (67.74%), Postives = 26/31 (83.87%), Query Frame = 0


Query: 4   NFGHQDFLELAVEDFNILQSIHHKELKELER 35
           NFG++DFL L+VEDFN  Q IH +EL+ELER
Sbjct: 462 NFGNKDFLSLSVEDFNRCQVIHREELRELER 492

[Close]

BLAST of MS011437 vs. ExPASy Swiss-Prot
Match: A0A1Z3GC64 (Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 8.5e-05
Identity = 21/31 (67.74%), Postives = 26/31 (83.87%), Query Frame = 0


Query: 4   NFGHQDFLELAVEDFNILQSIHHKELKELER 35
           NFG++DFL L+VEDFN  Q IH +EL+ELER
Sbjct: 462 NFGNKDFLSLSVEDFNRCQVIHREELRELER 492

[Close]

BLAST of MS011437 vs. ExPASy Swiss-Prot
Match: A0A1W6QDI6 (Terpene synthase 6, chloroplastic (Fragment) OS=Isodon rubescens OX=587669 GN=TPS6 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 8.5e-05
Identity = 21/31 (67.74%), Postives = 26/31 (83.87%), Query Frame = 0


Query: 4   NFGHQDFLELAVEDFNILQSIHHKELKELER 35
           NFG++DFL L+VEDFN  Q IH +EL+ELER
Sbjct: 352 NFGNKDFLSLSVEDFNRCQVIHREELRELER 382

[Close]

BLAST of MS011437 vs. ExPASy Swiss-Prot
Match: A0A2K9RFY0 (Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 PE=1 SV=1)

HSP 1 Score: 43.5 bits (101), Expect = 5.5e-04
Identity = 19/31 (61.29%), Postives = 27/31 (87.10%), Query Frame = 0


Query: 4   NFGHQDFLELAVEDFNILQSIHHKELKELER 35
           NFG++DFL L+V+D+N  Q+IH +EL+ELER
Sbjct: 459 NFGNKDFLFLSVKDYNECQAIHREELRELER 489

[Close]

BLAST of MS011437 vs. ExPASy TrEMBL
Match: A0A6J1D2L1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 7.0e-10
Identity = 33/34 (97.06%), Postives = 34/34 (100.00%), Query Frame = 0


Query: 1   SCLNFGHQDFLELAVEDFNILQSIHHKELKELER 35
           +CLNFGHQDFLELAVEDFNILQSIHHKELKELER
Sbjct: 497 TCLNFGHQDFLELAVEDFNILQSIHHKELKELER 530

[Close]

BLAST of MS011437 vs. ExPASy TrEMBL
Match: A0A6J1D2F0 (ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 7.0e-10
Identity = 33/34 (97.06%), Postives = 34/34 (100.00%), Query Frame = 0


Query: 1   SCLNFGHQDFLELAVEDFNILQSIHHKELKELER 35
           +CLNFGHQDFLELAVEDFNILQSIHHKELKELER
Sbjct: 495 TCLNFGHQDFLELAVEDFNILQSIHHKELKELER 528

[Close]

BLAST of MS011437 vs. ExPASy TrEMBL
Match: A0A6J1D1G1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 7.0e-10
Identity = 33/34 (97.06%), Postives = 34/34 (100.00%), Query Frame = 0


Query: 1   SCLNFGHQDFLELAVEDFNILQSIHHKELKELER 35
           +CLNFGHQDFLELAVEDFNILQSIHHKELKELER
Sbjct: 484 TCLNFGHQDFLELAVEDFNILQSIHHKELKELER 517

[Close]

BLAST of MS011437 vs. ExPASy TrEMBL
Match: Q9FRX5 (Ent-kaurene synthase OS=Cucumis sativus OX=3659 GN=CsKS1 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-07
Identity = 29/34 (85.29%), Postives = 31/34 (91.18%), Query Frame = 0


Query: 1   SCLNFGHQDFLELAVEDFNILQSIHHKELKELER 35
           +CLNFGHQDFLELAVEDFN LQ IH KELKELE+
Sbjct: 448 ACLNFGHQDFLELAVEDFNTLQGIHRKELKELEK 481

[Close]

BLAST of MS011437 vs. ExPASy TrEMBL
Match: A0A1S3BGJ9 (ent-kaur-16-ene synthase, chloroplastic isoform X3 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.9e-07
Identity = 29/34 (85.29%), Postives = 31/34 (91.18%), Query Frame = 0


Query: 1   SCLNFGHQDFLELAVEDFNILQSIHHKELKELER 35
           +CLNFGHQDFLELAVEDFN LQ IH KELKELE+
Sbjct: 384 ACLNFGHQDFLELAVEDFNTLQCIHRKELKELEK 417

The following BLAST results are available for this feature:

BLAST of MS011437 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147908.1	1.4e-09	97.06	ent-kaur-16-ene synthase, chloroplastic isoform X1 [Momordica charantia]	[more]
XP_022147910.1	1.4e-09	97.06	ent-kaur-16-ene synthase, chloroplastic isoform X3 [Momordica charantia]	[more]
XP_022147909.1	1.4e-09	97.06	ent-kaur-16-ene synthase, chloroplastic isoform X2 [Momordica charantia]	[more]
NP_001292675.1	3.0e-07	85.29	ent-kaur-16-ene synthase, chloroplastic [Cucumis sativus] >XP_004139844.2 ent-ka...	[more]
XP_011659051.1	3.0e-07	85.29	ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis sativus]	[more]

BLAST of MS011437 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39548	3.3e-09	82.35	Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
A0A3G1QTS7	8.5e-05	67.74	Ent-kaurene synthase 1, chloroplastic OS=Isodon eriocalyx OX=662907 GN=KS1 PE=1 ...	[more]
A0A1Z3GC64	8.5e-05	67.74	Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1...	[more]
A0A1W6QDI6	8.5e-05	67.74	Terpene synthase 6, chloroplastic (Fragment) OS=Isodon rubescens OX=587669 GN=TP...	[more]
A0A2K9RFY0	5.5e-04	61.29	Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 ...	[more]

BLAST of MS011437 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2L1	7.0e-10	97.06	ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Momordica charantia OX=367...	[more]
A0A6J1D2F0	7.0e-10	97.06	ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Momordica charantia OX=367...	[more]
A0A6J1D1G1	7.0e-10	97.06	ent-kaur-16-ene synthase, chloroplastic isoform X3 OS=Momordica charantia OX=367...	[more]
Q9FRX5	1.5e-07	85.29	Ent-kaurene synthase OS=Cucumis sativus OX=3659 GN=CsKS1 PE=2 SV=1	[more]
A0A1S3BGJ9	1.9e-07	85.29	ent-kaur-16-ene synthase, chloroplastic isoform X3 OS=Cucumis melo OX=3656 GN=LO...	[more]

BLAST of MS011437 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005630	Terpene synthase, metal-binding domain	PFAM	PF03936	Terpene_synth_C	coord: 11..34 e-value: 2.4E-6 score: 27.1
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 6..34 e-value: 9.7E-7 score: 30.1
IPR008949	Isoprenoid synthase domain superfamily	SUPERFAMILY	48576	Terpenoid synthases	coord: 8..34

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS011437.1	MS011437.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0010333	terpene synthase activity