MS011414 (gene) Bitter gourd (TR) v1

BLAST of MS011414 vs. NCBI nr
Match: XP_022133258.1 (protein misato homolog 1 isoform X1 [Momordica charantia])

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 568/600 (94.67%), Postives = 572/600 (95.33%), Query Frame = 0

Query: 1   MREIVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60
           MREIVT+QVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV
Sbjct: 1   MREIVTIQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60

Query: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQSL 120
           LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTH+TEPLKKNLFLQSL
Sbjct: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHNTEPLKKNLFLQSL 120

Query: 121 SEEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV 180
           SEEEQENSMNGKNSEQATSR EIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV
Sbjct: 121 SEEEQENSMNGKNSEQATSRIEIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV 180

Query: 181 DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDDSGGFSAIAGDFLE 240
           DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVD+SGGFSAIAGDFLE
Sbjct: 181 DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDNSGGFSAIAGDFLE 240

Query: 241 NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFMPVGLPS 300
           NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLF+PVGLPS
Sbjct: 241 NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFVPVGLPS 300

Query: 301 LSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMVR 360
           LSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMVR
Sbjct: 301 LSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMVR 360

Query: 361 MLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHHQSQASAICNTGKHLDQPLL 420
           MLSGQGRQNMVAILDVAMPAPSLC                           GKHLDQPLL
Sbjct: 361 MLSGQGRQNMVAILDVAMPAPSLC---------------------------GKHLDQPLL 420

Query: 421 GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH 480
           GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH
Sbjct: 421 GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH 480

Query: 481 LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL 540
           LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL
Sbjct: 481 LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL 540

Query: 541 PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD 600
           PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD
Sbjct: 541 PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD 573

BLAST of MS011414 vs. NCBI nr
Match: XP_022133259.1 (protein misato homolog 1 isoform X2 [Momordica charantia])

HSP 1 Score: 1015.8 bits (2625), Expect = 1.6e-292
Identity = 514/556 (92.45%), Postives = 521/556 (93.71%), Query Frame = 0

Query: 45  LNMDVLYRSGETQQGVLTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVS 104
           L +++L  +  T  GVLTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVS
Sbjct: 4   LRINILTWTYSTVLGVLTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVS 63

Query: 105 THHTEPLKKNLFLQSLSEEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSK 164
           TH+TEPLKKNLFLQSLSEEEQENSMNGKNSEQATSR EIQDKDIVECLEKDVTFWTDFSK
Sbjct: 64  THNTEPLKKNLFLQSLSEEEQENSMNGKNSEQATSRIEIQDKDIVECLEKDVTFWTDFSK 123

Query: 165 VHYHPQSLYQLNGLWVDVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFI 224
           VHYHPQSLYQLNGLWVDVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFI
Sbjct: 124 VHYHPQSLYQLNGLWVDVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFI 183

Query: 225 VDDSGGFSAIAGDFLENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFA 284
           VD+SGGFSAIAGDFLENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFA
Sbjct: 184 VDNSGGFSAIAGDFLENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFA 243

Query: 285 RLSSFCQLFMPVGLPSLSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTA 344
           RLSSFCQLF+PVGLPSLSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTA
Sbjct: 244 RLSSFCQLFVPVGLPSLSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTA 303

Query: 345 DSCYVSGAVHINDMVRMLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHHQSQ 404
           DSCYVSGAVHINDMVRMLSGQGRQNMVAILDVAMPAPSLC                    
Sbjct: 304 DSCYVSGAVHINDMVRMLSGQGRQNMVAILDVAMPAPSLC-------------------- 363

Query: 405 ASAICNTGKHLDQPLLGNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQ 464
                  GKHLDQPLLGNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQ
Sbjct: 364 -------GKHLDQPLLGNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQ 423

Query: 465 ICAAYEGSTERPMLCHLSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDV 524
           ICAAYEGSTERPMLCHLSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDV
Sbjct: 424 ICAAYEGSTERPMLCHLSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDV 483

Query: 525 HSIPMAARLRSSKAILPFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSK 584
           HSIPMAARLRSSKAILPFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSK
Sbjct: 484 HSIPMAARLRSSKAILPFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSK 532

Query: 585 MVLALNPHSQSSSDSD 601
           MVLALNPHSQSSSDSD
Sbjct: 544 MVLALNPHSQSSSDSD 532

BLAST of MS011414 vs. NCBI nr
Match: XP_038883553.1 (protein misato homolog 1 isoform X1 [Benincasa hispida] >XP_038883554.1 protein misato homolog 1 isoform X1 [Benincasa hispida] >XP_038883555.1 protein misato homolog 1 isoform X1 [Benincasa hispida] >XP_038883559.1 protein misato homolog 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1007.3 bits (2603), Expect = 5.6e-290
Identity = 507/600 (84.50%), Postives = 534/600 (89.00%), Query Frame = 0

Query: 1   MREIVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60
           MRE+VTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV
Sbjct: 1   MRELVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60

Query: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQSL 120
           LTYTPRLVSVGFKGGLGSVSARGTLYNDDA  PSD+ITWRGNV+THHTE  KKNLFLQSL
Sbjct: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDADRPSDIITWRGNVATHHTEQRKKNLFLQSL 120

Query: 121 SEEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV 180
           SEEEQENS+NGKN+     R EI+DKDIVECLEKDVTFWTDFSKVHYHPQSLYQ NG WV
Sbjct: 121 SEEEQENSVNGKNN----GRGEIEDKDIVECLEKDVTFWTDFSKVHYHPQSLYQFNGSWV 180

Query: 181 DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDDSGGFSAIAGDFLE 240
           D QEF+NYGIGKESF+WS Q E+IDERLRFFVEECDHIQGFQFIVDDSGGFSAIAGDFLE
Sbjct: 181 DAQEFNNYGIGKESFTWSLQGEEIDERLRFFVEECDHIQGFQFIVDDSGGFSAIAGDFLE 240

Query: 241 NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFMPVGLPS 300
           +VADEYSNTPVLLYSVRSP S S  RENKRQ VSR+LHDAVSFARLSSFCQL +PVGLPS
Sbjct: 241 SVADEYSNTPVLLYSVRSPTSPSTLRENKRQIVSRDLHDAVSFARLSSFCQLCVPVGLPS 300

Query: 301 LSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMVR 360
           LSRSKA ++LCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPT DSCYVSGAVH+NDMVR
Sbjct: 301 LSRSKATSYLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTTDSCYVSGAVHVNDMVR 360

Query: 361 MLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHHQSQASAICNTGKHLDQPLL 420
           ML+GQGRQNMVAILD++MPAPSL                           +GKHL Q LL
Sbjct: 361 MLAGQGRQNMVAILDISMPAPSL---------------------------SGKHLGQSLL 420

Query: 421 GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH 480
           G+LQPLTPEV EDAED LALESMTVHGVFG GG+RAS+ ELKDQI AAYEG + RPMLCH
Sbjct: 421 GSLQPLTPEVKEDAEDLLALESMTVHGVFGSGGERASISELKDQISAAYEGESVRPMLCH 480

Query: 481 LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL 540
           LSA QCPLP+PLPFPSIFGNLVGQHG+LLATPIS AS RGSLDVHSIPMAARLRSS AIL
Sbjct: 481 LSAAQCPLPVPLPFPSIFGNLVGQHGQLLATPISSASFRGSLDVHSIPMAARLRSSNAIL 540

Query: 541 PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD 600
           PFLERRLTNLLRHGT QGSSAA LLR+WGFGREELEDMGES+SKMVLALNPHS SSSDSD
Sbjct: 541 PFLERRLTNLLRHGTVQGSSAAPLLRSWGFGREELEDMGESLSKMVLALNPHSLSSSDSD 569

BLAST of MS011414 vs. NCBI nr
Match: XP_022133260.1 (protein misato homolog 1 isoform X3 [Momordica charantia])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.2e-289
Identity = 518/600 (86.33%), Postives = 523/600 (87.17%), Query Frame = 0

Query: 1   MREIVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60
           MREIVT+QVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV
Sbjct: 1   MREIVTIQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60

Query: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQSL 120
           LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTH+TEPLKKNLFLQSL
Sbjct: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHNTEPLKKNLFLQSL 120

Query: 121 SEEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV 180
           SEEEQENSMNGKNSEQATSR EIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV
Sbjct: 121 SEEEQENSMNGKNSEQATSRIEIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV 180

Query: 181 DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDDSGGFSAIAGDFLE 240
           DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVD+SGGFSAIAGDFLE
Sbjct: 181 DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDNSGGFSAIAGDFLE 240

Query: 241 NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFMPVGLPS 300
           NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLF+PVGLPS
Sbjct: 241 NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFVPVGLPS 300

Query: 301 LSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMVR 360
           LSR                                                 +HINDMVR
Sbjct: 301 LSR-------------------------------------------------IHINDMVR 360

Query: 361 MLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHHQSQASAICNTGKHLDQPLL 420
           MLSGQGRQNMVAILDVAMPAPSLC                           GKHLDQPLL
Sbjct: 361 MLSGQGRQNMVAILDVAMPAPSLC---------------------------GKHLDQPLL 420

Query: 421 GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH 480
           GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH
Sbjct: 421 GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH 480

Query: 481 LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL 540
           LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL
Sbjct: 481 LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL 524

Query: 541 PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD 600
           PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD
Sbjct: 541 PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD 524

BLAST of MS011414 vs. NCBI nr
Match: XP_008440245.1 (PREDICTED: protein misato homolog 1 isoform X1 [Cucumis melo])

HSP 1 Score: 990.7 bits (2560), Expect = 5.4e-285
Identity = 498/600 (83.00%), Postives = 530/600 (88.33%), Query Frame = 0

Query: 1   MREIVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60
           MRE+VTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV
Sbjct: 1   MRELVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60

Query: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQSL 120
           LTYTPRLVSVGFKGGLGSVSARGTLYNDD  TPSD++TWRG+V+THHTEP KKNLFLQSL
Sbjct: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDVDTPSDIVTWRGSVATHHTEPRKKNLFLQSL 120

Query: 121 SEEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV 180
           SEEEQEN ++G+NS    SR EI+DKDIV+CLE DVTFWTDFSKVHYHPQSLYQL G WV
Sbjct: 121 SEEEQENLVDGENS----SRGEIEDKDIVKCLENDVTFWTDFSKVHYHPQSLYQLAGSWV 180

Query: 181 DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDDSGGFSAIAGDFLE 240
           D QEFDNYGIGKESFSWS Q EDIDERLRFFVEECDHIQGFQFIV+DSGGFSA+AGDFLE
Sbjct: 181 DAQEFDNYGIGKESFSWSLQGEDIDERLRFFVEECDHIQGFQFIVEDSGGFSAVAGDFLE 240

Query: 241 NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFMPVGLPS 300
           +VADEYSNTPVLLYSVRSP S ++ RENKRQ VSR+LHDA+SFARLSSFCQL++PVGLPS
Sbjct: 241 SVADEYSNTPVLLYSVRSPSS-NVLRENKRQIVSRDLHDAISFARLSSFCQLYVPVGLPS 300

Query: 301 LSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMVR 360
           LSRSKA THLCIDDQKPYHCSAVYAAALHSIGLP RMEA GPT DSCYVSGA+H+NDMVR
Sbjct: 301 LSRSKAATHLCIDDQKPYHCSAVYAAALHSIGLPFRMEAQGPTTDSCYVSGALHVNDMVR 360

Query: 361 MLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHHQSQASAICNTGKHLDQPLL 420
           ML+GQGRQNMVAILDVAMPAP+L                             KHL Q LL
Sbjct: 361 MLAGQGRQNMVAILDVAMPAPTL---------------------------LEKHLGQSLL 420

Query: 421 GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH 480
           G+LQPLTPEV EDAED LALESMTVHGVF  GGDRASV ELKDQI AAYEG T RPMLCH
Sbjct: 421 GSLQPLTPEVKEDAEDLLALESMTVHGVFESGGDRASVSELKDQISAAYEGETVRPMLCH 480

Query: 481 LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL 540
           LSA QCPLP+PL FPSIFGNLVGQHG+LLATPISG SSRGSLDV+SIPMAARLRSS A+L
Sbjct: 481 LSAAQCPLPVPLSFPSIFGNLVGQHGQLLATPISGVSSRGSLDVYSIPMAARLRSSNAVL 540

Query: 541 PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD 600
           PF+ERRLTNLLRHGT QGSSA  LLR+WGFGREELEDMGES+SKMVLALNPHS +SSDSD
Sbjct: 541 PFIERRLTNLLRHGTVQGSSATPLLRSWGFGREELEDMGESLSKMVLALNPHSLTSSDSD 568

BLAST of MS011414 vs. ExPASy Swiss-Prot
Match: Q5RF82 (Protein misato homolog 1 OS=Pongo pygmaeus OX=9600 GN=MSTO1 PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 6.0e-45
Identity = 118/356 (33.15%), Postives = 180/356 (50.56%), Query Frame = 0

Query: 2   REIVTVQVGEFANFVGSHFWNFQDELIGLAAD---PLGDAVFKNQHLNMDVLYRSGETQQ 61
           RE++T+Q+G FA FVG+H+WN QD  +G A D   PLG+       L  DVLYR+G T  
Sbjct: 6   REVLTLQLGHFAGFVGAHWWNQQDAALGQATDSKEPLGE-------LCPDVLYRTGRTLH 65

Query: 62  GVLTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQ 121
           G  TYTPRL+ +  KG L S+   G LY D     +  I W+G ++TH  E   KN +LQ
Sbjct: 66  GQETYTPRLILMDLKGSLSSLKEEGGLYRDKQLDAA--IAWQGKLTTHKEELYPKNPYLQ 125

Query: 122 SLSEEE----------QENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYH 181
                E           ++  NGK S   T+      K ++   E  +  W+DF +VH H
Sbjct: 126 DFLSAEGVLSSDGVWRVKSIPNGKGSPPLTT--ATTPKPLIP-TEASIRVWSDFLRVHLH 185

Query: 182 PQSLYQLNGLWVDVQ--EFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVD 241
           P+S+  +     D +    + +G G+        +E++++RL F+VEECD++QGFQ + D
Sbjct: 186 PRSICMIQKYNHDGEAGRLEAFGQGESVLKEPKYQEELEDRLHFYVEECDYLQGFQILCD 245

Query: 242 DSGGFSAIAGDFLENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARL 301
              GFS +     E + DEYS   ++ + +  PG Y   R   ++ + R L+ A     L
Sbjct: 246 LHDGFSGVGAKAAELLQDEYSGRGIITWGL-LPGPY--HRGEAQRNIYRLLNTAFGLVHL 305

Query: 302 SSFCQLFMPVGLPSLSRSKA-----ITHLCIDDQKPYHCSAVYAAALHSIGLPLRM 338
           ++   L  P+ L      +        +L  D   P+HCSA+ A AL ++ +P R+
Sbjct: 306 TAHSSLVCPLSLGGSLGLRPEPPVNFPYLHYDATLPFHCSAILATALDTVTVPYRL 346

BLAST of MS011414 vs. ExPASy Swiss-Prot
Match: A5D9D4 (Protein misato homolog 1 OS=Bos taurus OX=9913 GN=MSTO1 PE=2 SV=2)

HSP 1 Score: 182.2 bits (461), Expect = 1.8e-44
Identity = 158/560 (28.21%), Postives = 251/560 (44.82%), Query Frame = 0

Query: 2   REIVTVQVGEFANFVGSHFWNFQDELIGL---AADPLGDAVFKNQHLNMDVLYRSGETQQ 61
           RE++T+Q+G FA FVG+H+WN QD  +     A +P G+       L  DVLYR+G T  
Sbjct: 6   REVLTLQLGHFAGFVGAHWWNQQDAALCRPTDAKEPPGE-------LCPDVLYRTGRTLH 65

Query: 62  GVLTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQ 121
           G  TYTPRL+ +  KG L S+   G LY D     +  I W+G ++TH  E   KN +LQ
Sbjct: 66  GQETYTPRLILMDLKGSLSSLKQEGGLYRDKQLDAA--IAWQGKLTTHKEELYPKNPYLQ 125

Query: 122 SLSEEE----------QENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYH 181
            L   E           ++  NGK     T+   I  K ++   E  +  W+DF +VH H
Sbjct: 126 DLLSAEGVLSSDGTWRVKSIPNGKGLPPFTN--AITPKPVMP-TEGSIRVWSDFLRVHLH 185

Query: 182 PQSLYQLNGLWVDVQ--EFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVD 241
           P+S+  ++    D +    + +G G+         E++++RL F+VEECD++QGFQ + D
Sbjct: 186 PRSICMIHKYNHDGEAGRLEAFGQGESILKEPKYLEELEDRLHFYVEECDYLQGFQILCD 245

Query: 242 DSGGFSAIAGDFLENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARL 301
              GFS +     E + DEYS   ++ + +  PG Y +    K   + R L+ A     L
Sbjct: 246 LHDGFSGLGAKAAELLQDEYSGRGIITWGL-LPGPYRLGELQK--NIYRLLNTAFGLVHL 305

Query: 302 SSFCQLFMPVGLPSLSRSK-----AITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHG 361
           S+   L  P+ L      +     +   L  D   P+HC A+ A AL ++ +P R+ +  
Sbjct: 306 SAHSSLVCPLSLGGSLGLRPEPPVSFPLLQYDATLPFHCGAILATALDTVTVPYRLRS-S 365

Query: 362 PTADSCYVSGAVHINDMVRMLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHH 421
           P +        VH+ D   ML+  G++ + A   +  P+     +  ++    +   +  
Sbjct: 366 PVS-------MVHLAD---MLNFSGKKVVTAGATIPFPSVPSQSLPDTLMQLGEATPWTP 425

Query: 422 QSQASAICNTGKHLDQPLLGNLQPL--TPEVAEDAEDPLALESMTVHGVFGLGGDRASVP 481
            S       T       +L  L     T ++A     P  L +          G+     
Sbjct: 426 LSACGDPSGTCCFAQSVVLRGLDRAHHTSQLAPGTPLPSPLHACAT-------GEEVLAQ 485

Query: 482 ELKDQICAAYEGSTERPMLCHLSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSR 540
            L+ Q       S       HL  T C + +P P+PS+F + + QHG +L    +G +  
Sbjct: 486 YLQQQQPRVRSSS-------HLLLTPCKV-VP-PYPSLFSSSLSQHGSVLDGLPTGTA-- 517

BLAST of MS011414 vs. ExPASy Swiss-Prot
Match: Q2YDW2 (Protein misato homolog 1 OS=Mus musculus OX=10090 GN=Msto1 PE=1 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 5.1e-44
Identity = 130/401 (32.42%), Postives = 194/401 (48.38%), Query Frame = 0

Query: 2   REIVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGVL 61
           RE++T+Q+G FA FVG+H+WN QD  +G  A+           L  DVLYR+G T  G  
Sbjct: 6   REVLTLQLGHFAGFVGAHWWNQQDAALGRMAEDEESP----GELCPDVLYRTGRTLHGQE 65

Query: 62  TYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQSLS 121
           TYTPRL+ +  KG L ++   G LY D     +  + W+G +STH  +   KN  LQ L 
Sbjct: 66  TYTPRLILMDLKGSLNTLKEEGNLYRDRQLEAA--VAWQGKLSTHREDAHPKNPNLQGLL 125

Query: 122 EEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWVD 181
             E   S +G     A   + IQ  +I    E  +  W+DF +VH HP+S+  ++    D
Sbjct: 126 SAEGVRSSDG-----AWRAKLIQ--NIQNGKENSIKVWSDFLRVHLHPRSICVIHKYHHD 185

Query: 182 VQ--EFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDDSGGFSAIAGDFL 241
            +    + +G G+         E++++RL F+VEECD++QGFQ + D   GFS +     
Sbjct: 186 GETGRLEAFGQGESVLKEPRYLEELEDRLHFYVEECDYLQGFQLLCDLHDGFSGVGAKTA 245

Query: 242 ENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFMPVGLP 301
           E + DEY+   VL + +  PG YS+    K   + R L+ A     L+ +     P+ L 
Sbjct: 246 ELLQDEYAGRGVLTWGL-LPGPYSLGEPQK--NIYRLLNTAFGLVHLTGYSSFVCPLSLG 305

Query: 302 SL--SRSKA---ITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPT----ADSCYVS 361
                R K       L  D   P+HCSA+ A AL ++ +P R+ +   T    AD    S
Sbjct: 306 GNLGLRPKPPVNFPSLHYDATLPFHCSAILATALDTVTVPYRLRSSMVTMAHLADVLSFS 365

Query: 362 GAVHINDMV----RMLSGQGRQNMVAILDVAMPAPSL--CG 386
           G   +         ++ GQ   +++  L  A P  SL  CG
Sbjct: 366 GKKVVTAEAIIPFPLVRGQSLPDILTQLGEATPWTSLSACG 390

BLAST of MS011414 vs. ExPASy Swiss-Prot
Match: Q9BUK6 (Protein misato homolog 1 OS=Homo sapiens OX=9606 GN=MSTO1 PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 8.7e-44
Identity = 116/356 (32.58%), Postives = 179/356 (50.28%), Query Frame = 0

Query: 2   REIVTVQVGEFANFVGSHFWNFQDELIGLAAD---PLGDAVFKNQHLNMDVLYRSGETQQ 61
           RE++T+Q+G FA FVG+H+WN QD  +G A D   P G+       L  DVLYR+G T  
Sbjct: 6   REVLTLQLGHFAGFVGAHWWNQQDAALGRATDSKEPPGE-------LCPDVLYRTGRTLH 65

Query: 62  GVLTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQ 121
           G  TYTPRL+ +  KG L S+   G LY D     +  I W+G ++TH  E   KN +LQ
Sbjct: 66  GQETYTPRLILMDLKGSLSSLKEEGGLYRDKQLDAA--IAWQGKLTTHKEELYPKNPYLQ 125

Query: 122 SLSEEE----------QENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYH 181
                E           ++  NGK S    +      K ++   E  +  W+DF +VH H
Sbjct: 126 DFLSAEGVLSSDGVWRVKSIPNGKGSSPLPT--ATTPKPLIP-TEASIRVWSDFLRVHLH 185

Query: 182 PQSLYQLNGLWVDVQ--EFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVD 241
           P+S+  +     D +    + +G G+        +E++++RL F+VEECD++QGFQ + D
Sbjct: 186 PRSICMIQKYNHDGEAGRLEAFGQGESVLKEPKYQEELEDRLHFYVEECDYLQGFQILCD 245

Query: 242 DSGGFSAIAGDFLENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARL 301
              GFS +     E + DEYS   ++ + +  PG Y   R   ++ + R L+ A     L
Sbjct: 246 LHDGFSGVGAKAAELLQDEYSGRGIITWGL-LPGPY--HRGEAQRNIYRLLNTAFGLVHL 305

Query: 302 SSFCQLFMPVGLPSLSRSK-----AITHLCIDDQKPYHCSAVYAAALHSIGLPLRM 338
           ++   L  P+ L      +     +  +L  D   P+HCSA+ A AL ++ +P R+
Sbjct: 306 TAHSSLVCPLSLGGSLGLRPEPPVSFPYLHYDATLPFHCSAILATALDTVTVPYRL 346

BLAST of MS011414 vs. ExPASy Swiss-Prot
Match: Q4R681 (Protein misato homolog 1 OS=Macaca fascicularis OX=9541 GN=MSTO1 PE=2 SV=2)

HSP 1 Score: 177.9 bits (450), Expect = 3.3e-43
Identity = 123/395 (31.14%), Postives = 190/395 (48.10%), Query Frame = 0

Query: 2   REIVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGVL 61
           RE++T+Q+G FA FVG+H+WN QD  +G A     DA      L  DVLYR+G T  G  
Sbjct: 6   REVLTLQLGHFAGFVGAHWWNQQDAALGRAT----DAKESPGELCPDVLYRTGRTLHGQD 65

Query: 62  TYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQSLS 121
           T TPRL+ +  KG L S+   G LY D     +  I W+G ++TH  E   KN +LQ   
Sbjct: 66  TCTPRLILMDLKGSLSSLKEEGGLYRDKQLDAA--IAWQGKLTTHKEELCPKNPYLQDFL 125

Query: 122 EEE----------QENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYHPQS 181
             E           ++  NGK S   T+      K ++   E  +  W+DF +VH HP+S
Sbjct: 126 STEGVLSSDGVWRVKSIPNGKGSPPLTT--ATTPKPLIP-TEASIRVWSDFLRVHLHPRS 185

Query: 182 LYQLNGLWVDVQ--EFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDDSG 241
           +  +     D +    + +G G+        +E++++RL F+VEECD++QGFQ + D   
Sbjct: 186 ICMIQKYNHDGEAGRLEAFGQGESVLKEPKYQEELEDRLHFYVEECDYLQGFQILCDLHD 245

Query: 242 GFSAIAGDFLENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSF 301
           GFS +     E + DEYS   ++ + +  PG Y   R   ++ + R L+ A     L++ 
Sbjct: 246 GFSGVGAKAAELLQDEYSGRGIITWGL-LPGPY--HRGEAQRNIYRLLNTAFGLVHLTAH 305

Query: 302 CQLFMPVGLPSLSRSKA-----ITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTA 361
             L  P+ L      +        +L  D   P+HCSA+ A AL ++ +P R+       
Sbjct: 306 SSLVCPLSLGGSLGLRPEPPVNFPYLHYDATLPFHCSAILATALDTVTVPYRL------- 365

Query: 362 DSCYVSGAVHINDMVRMLSGQGRQNMVAILDVAMP 380
                S  V +  +  MLS  G++ + A   +  P
Sbjct: 366 ----CSSPVSMVHLADMLSFCGKKVVTAGATIPFP 377

BLAST of MS011414 vs. ExPASy TrEMBL
Match: A0A6J1BYL9 (protein misato homolog 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005896 PE=3 SV=1)

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 568/600 (94.67%), Postives = 572/600 (95.33%), Query Frame = 0

Query: 1   MREIVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60
           MREIVT+QVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV
Sbjct: 1   MREIVTIQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60

Query: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQSL 120
           LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTH+TEPLKKNLFLQSL
Sbjct: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHNTEPLKKNLFLQSL 120

Query: 121 SEEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV 180
           SEEEQENSMNGKNSEQATSR EIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV
Sbjct: 121 SEEEQENSMNGKNSEQATSRIEIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV 180

Query: 181 DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDDSGGFSAIAGDFLE 240
           DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVD+SGGFSAIAGDFLE
Sbjct: 181 DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDNSGGFSAIAGDFLE 240

Query: 241 NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFMPVGLPS 300
           NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLF+PVGLPS
Sbjct: 241 NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFVPVGLPS 300

Query: 301 LSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMVR 360
           LSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMVR
Sbjct: 301 LSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMVR 360

Query: 361 MLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHHQSQASAICNTGKHLDQPLL 420
           MLSGQGRQNMVAILDVAMPAPSLC                           GKHLDQPLL
Sbjct: 361 MLSGQGRQNMVAILDVAMPAPSLC---------------------------GKHLDQPLL 420

Query: 421 GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH 480
           GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH
Sbjct: 421 GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH 480

Query: 481 LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL 540
           LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL
Sbjct: 481 LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL 540

Query: 541 PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD 600
           PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD
Sbjct: 541 PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD 573

BLAST of MS011414 vs. ExPASy TrEMBL
Match: A0A6J1BVH8 (protein misato homolog 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005896 PE=3 SV=1)

HSP 1 Score: 1015.8 bits (2625), Expect = 7.6e-293
Identity = 514/556 (92.45%), Postives = 521/556 (93.71%), Query Frame = 0

Query: 45  LNMDVLYRSGETQQGVLTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVS 104
           L +++L  +  T  GVLTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVS
Sbjct: 4   LRINILTWTYSTVLGVLTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVS 63

Query: 105 THHTEPLKKNLFLQSLSEEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSK 164
           TH+TEPLKKNLFLQSLSEEEQENSMNGKNSEQATSR EIQDKDIVECLEKDVTFWTDFSK
Sbjct: 64  THNTEPLKKNLFLQSLSEEEQENSMNGKNSEQATSRIEIQDKDIVECLEKDVTFWTDFSK 123

Query: 165 VHYHPQSLYQLNGLWVDVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFI 224
           VHYHPQSLYQLNGLWVDVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFI
Sbjct: 124 VHYHPQSLYQLNGLWVDVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFI 183

Query: 225 VDDSGGFSAIAGDFLENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFA 284
           VD+SGGFSAIAGDFLENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFA
Sbjct: 184 VDNSGGFSAIAGDFLENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFA 243

Query: 285 RLSSFCQLFMPVGLPSLSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTA 344
           RLSSFCQLF+PVGLPSLSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTA
Sbjct: 244 RLSSFCQLFVPVGLPSLSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTA 303

Query: 345 DSCYVSGAVHINDMVRMLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHHQSQ 404
           DSCYVSGAVHINDMVRMLSGQGRQNMVAILDVAMPAPSLC                    
Sbjct: 304 DSCYVSGAVHINDMVRMLSGQGRQNMVAILDVAMPAPSLC-------------------- 363

Query: 405 ASAICNTGKHLDQPLLGNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQ 464
                  GKHLDQPLLGNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQ
Sbjct: 364 -------GKHLDQPLLGNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQ 423

Query: 465 ICAAYEGSTERPMLCHLSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDV 524
           ICAAYEGSTERPMLCHLSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDV
Sbjct: 424 ICAAYEGSTERPMLCHLSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDV 483

Query: 525 HSIPMAARLRSSKAILPFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSK 584
           HSIPMAARLRSSKAILPFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSK
Sbjct: 484 HSIPMAARLRSSKAILPFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSK 532

Query: 585 MVLALNPHSQSSSDSD 601
           MVLALNPHSQSSSDSD
Sbjct: 544 MVLALNPHSQSSSDSD 532

BLAST of MS011414 vs. ExPASy TrEMBL
Match: A0A6J1BUS3 (protein misato homolog 1 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111005896 PE=3 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 6.0e-290
Identity = 518/600 (86.33%), Postives = 523/600 (87.17%), Query Frame = 0

Query: 1   MREIVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60
           MREIVT+QVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV
Sbjct: 1   MREIVTIQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60

Query: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQSL 120
           LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTH+TEPLKKNLFLQSL
Sbjct: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHNTEPLKKNLFLQSL 120

Query: 121 SEEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV 180
           SEEEQENSMNGKNSEQATSR EIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV
Sbjct: 121 SEEEQENSMNGKNSEQATSRIEIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV 180

Query: 181 DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDDSGGFSAIAGDFLE 240
           DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVD+SGGFSAIAGDFLE
Sbjct: 181 DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDNSGGFSAIAGDFLE 240

Query: 241 NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFMPVGLPS 300
           NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLF+PVGLPS
Sbjct: 241 NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFVPVGLPS 300

Query: 301 LSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMVR 360
           LSR                                                 +HINDMVR
Sbjct: 301 LSR-------------------------------------------------IHINDMVR 360

Query: 361 MLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHHQSQASAICNTGKHLDQPLL 420
           MLSGQGRQNMVAILDVAMPAPSLC                           GKHLDQPLL
Sbjct: 361 MLSGQGRQNMVAILDVAMPAPSLC---------------------------GKHLDQPLL 420

Query: 421 GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH 480
           GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH
Sbjct: 421 GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH 480

Query: 481 LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL 540
           LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL
Sbjct: 481 LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL 524

Query: 541 PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD 600
           PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD
Sbjct: 541 PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD 524

BLAST of MS011414 vs. ExPASy TrEMBL
Match: A0A5D3CMX3 (Protein misato-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004990 PE=3 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 2.6e-285
Identity = 498/600 (83.00%), Postives = 530/600 (88.33%), Query Frame = 0

Query: 1   MREIVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60
           MRE+VTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV
Sbjct: 75  MRELVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 134

Query: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQSL 120
           LTYTPRLVSVGFKGGLGSVSARGTLYNDD  TPSD++TWRG+V+THHTEP KKNLFLQSL
Sbjct: 135 LTYTPRLVSVGFKGGLGSVSARGTLYNDDVDTPSDIVTWRGSVATHHTEPRKKNLFLQSL 194

Query: 121 SEEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV 180
           SEEEQEN ++G+NS    SR EI+DKDIV+CLE DVTFWTDFSKVHYHPQSLYQL G WV
Sbjct: 195 SEEEQENLVDGENS----SRGEIEDKDIVKCLENDVTFWTDFSKVHYHPQSLYQLAGSWV 254

Query: 181 DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDDSGGFSAIAGDFLE 240
           D QEFDNYGIGKESFSWS Q EDIDERLRFFVEECDHIQGFQFIV+DSGGFSA+AGDFLE
Sbjct: 255 DAQEFDNYGIGKESFSWSLQGEDIDERLRFFVEECDHIQGFQFIVEDSGGFSAVAGDFLE 314

Query: 241 NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFMPVGLPS 300
           +VADEYSNTPVLLYSVRSP S ++ RENKRQ VSR+LHDA+SFARLSSFCQL++PVGLPS
Sbjct: 315 SVADEYSNTPVLLYSVRSPSS-NVLRENKRQIVSRDLHDAISFARLSSFCQLYVPVGLPS 374

Query: 301 LSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMVR 360
           LSRSKA THLCIDDQKPYHCSAVYAAALHSIGLP RMEA GPT DSCYVSGA+H+NDMVR
Sbjct: 375 LSRSKAATHLCIDDQKPYHCSAVYAAALHSIGLPFRMEAQGPTTDSCYVSGALHVNDMVR 434

Query: 361 MLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHHQSQASAICNTGKHLDQPLL 420
           ML+GQGRQNMVAILDVAMPAP+L                             KHL Q LL
Sbjct: 435 MLAGQGRQNMVAILDVAMPAPTL---------------------------LEKHLGQSLL 494

Query: 421 GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH 480
           G+LQPLTPEV EDAED LALESMTVHGVF  GGDRASV ELKDQI AAYEG T RPMLCH
Sbjct: 495 GSLQPLTPEVKEDAEDLLALESMTVHGVFESGGDRASVSELKDQISAAYEGETVRPMLCH 554

Query: 481 LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL 540
           LSA QCPLP+PL FPSIFGNLVGQHG+LLATPISG SSRGSLDV+SIPMAARLRSS A+L
Sbjct: 555 LSAAQCPLPVPLSFPSIFGNLVGQHGQLLATPISGVSSRGSLDVYSIPMAARLRSSNAVL 614

Query: 541 PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD 600
           PF+ERRLTNLLRHGT QGSSA  LLR+WGFGREELEDMGES+SKMVLALNPHS +SSDSD
Sbjct: 615 PFIERRLTNLLRHGTVQGSSATPLLRSWGFGREELEDMGESLSKMVLALNPHSLTSSDSD 642

BLAST of MS011414 vs. ExPASy TrEMBL
Match: A0A1S3B0M6 (protein misato homolog 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484759 PE=3 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 2.6e-285
Identity = 498/600 (83.00%), Postives = 530/600 (88.33%), Query Frame = 0

Query: 1   MREIVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60
           MRE+VTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV
Sbjct: 1   MRELVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60

Query: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSDVITWRGNVSTHHTEPLKKNLFLQSL 120
           LTYTPRLVSVGFKGGLGSVSARGTLYNDD  TPSD++TWRG+V+THHTEP KKNLFLQSL
Sbjct: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDVDTPSDIVTWRGSVATHHTEPRKKNLFLQSL 120

Query: 121 SEEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLWV 180
           SEEEQEN ++G+NS    SR EI+DKDIV+CLE DVTFWTDFSKVHYHPQSLYQL G WV
Sbjct: 121 SEEEQENLVDGENS----SRGEIEDKDIVKCLENDVTFWTDFSKVHYHPQSLYQLAGSWV 180

Query: 181 DVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDDSGGFSAIAGDFLE 240
           D QEFDNYGIGKESFSWS Q EDIDERLRFFVEECDHIQGFQFIV+DSGGFSA+AGDFLE
Sbjct: 181 DAQEFDNYGIGKESFSWSLQGEDIDERLRFFVEECDHIQGFQFIVEDSGGFSAVAGDFLE 240

Query: 241 NVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFMPVGLPS 300
           +VADEYSNTPVLLYSVRSP S ++ RENKRQ VSR+LHDA+SFARLSSFCQL++PVGLPS
Sbjct: 241 SVADEYSNTPVLLYSVRSPSS-NVLRENKRQIVSRDLHDAISFARLSSFCQLYVPVGLPS 300

Query: 301 LSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMVR 360
           LSRSKA THLCIDDQKPYHCSAVYAAALHSIGLP RMEA GPT DSCYVSGA+H+NDMVR
Sbjct: 301 LSRSKAATHLCIDDQKPYHCSAVYAAALHSIGLPFRMEAQGPTTDSCYVSGALHVNDMVR 360

Query: 361 MLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHHQSQASAICNTGKHLDQPLL 420
           ML+GQGRQNMVAILDVAMPAP+L                             KHL Q LL
Sbjct: 361 MLAGQGRQNMVAILDVAMPAPTL---------------------------LEKHLGQSLL 420

Query: 421 GNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTERPMLCH 480
           G+LQPLTPEV EDAED LALESMTVHGVF  GGDRASV ELKDQI AAYEG T RPMLCH
Sbjct: 421 GSLQPLTPEVKEDAEDLLALESMTVHGVFESGGDRASVSELKDQISAAYEGETVRPMLCH 480

Query: 481 LSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSKAIL 540
           LSA QCPLP+PL FPSIFGNLVGQHG+LLATPISG SSRGSLDV+SIPMAARLRSS A+L
Sbjct: 481 LSAAQCPLPVPLSFPSIFGNLVGQHGQLLATPISGVSSRGSLDVYSIPMAARLRSSNAVL 540

Query: 541 PFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSSDSD 600
           PF+ERRLTNLLRHGT QGSSA  LLR+WGFGREELEDMGES+SKMVLALNPHS +SSDSD
Sbjct: 541 PFIERRLTNLLRHGTVQGSSATPLLRSWGFGREELEDMGESLSKMVLALNPHSLTSSDSD 568

BLAST of MS011414 vs. TAIR 10
Match: AT4G37190.1 (LOCATED IN: cytosol, plasma membrane; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Beta tubulin, autoregulation binding site (InterPro:IPR013838), Misato Segment II, myosin-like (InterPro:IPR019605), Tubulin/FtsZ, N-terminal (InterPro:IPR019746); Has 345 Blast hits to 341 proteins in 161 species: Archae - 0; Bacteria - 0; Metazoa - 131; Fungi - 140; Plants - 55; Viruses - 0; Other Eukaryotes - 19 (source: NCBI BLink). )

HSP 1 Score: 673.3 bits (1736), Expect = 1.8e-193
Identity = 351/603 (58.21%), Postives = 444/603 (73.63%), Query Frame = 0

Query: 1   MREIVTVQVGEFANFVGSHFWNFQDELIGLAADPLGDAVFKNQHLNMDVLYRSGETQQGV 60
           MREIVT+QVGEFANFVGSHFWNFQDEL+GLA+DP  D +F+N +L+MDVLYRSGETQQGV
Sbjct: 1   MREIVTIQVGEFANFVGSHFWNFQDELLGLASDPESDPIFRNHNLDMDVLYRSGETQQGV 60

Query: 61  LTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSD-VITWRGNVSTHHTEPLKKNLFLQS 120
            TYTPRLVSV  KG LG++S+RGTLYN+ + + SD   TW G+V T  +EP K+NLFLQS
Sbjct: 61  ATYTPRLVSVNLKGALGTMSSRGTLYNEGSSSRSDSSATWFGDVDTQRSEPRKRNLFLQS 120

Query: 121 LSEEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKVHYHPQSLYQLNGLW 180
           L EEE    + GK        +EI+DKDIV CL+++V  WTDFSK HYHPQSLY+LNGLW
Sbjct: 121 LYEEEH---VVGKE-----KAKEIEDKDIVGCLDEEVECWTDFSKSHYHPQSLYELNGLW 180

Query: 181 VDVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIVDDSGGFSAIAGDFL 240
           +D Q F+NYGIGK+ FS +S+ E+I +RLRFFVEECDHIQG +F+VDDSGGFSA+A DFL
Sbjct: 181 MDSQAFNNYGIGKDVFSEASRGEEICDRLRFFVEECDHIQGIKFLVDDSGGFSAVAADFL 240

Query: 241 ENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFARLSSFCQLFMPVGLP 300
           EN+ADEY+N PVLLYSVR+P    M + + ++TVS  LHDA+SF+RLSSFC+LF P+GLP
Sbjct: 241 ENMADEYTNVPVLLYSVRTP----MSQMSSKKTVSNKLHDAISFSRLSSFCKLFTPIGLP 300

Query: 301 SLSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTADSCYVSGAVHINDMV 360
           SL+ SKA   L + D+KPY  SAVYAAALHS  +P RM+    ++DS  VS ++ +N +V
Sbjct: 301 SLTGSKASKFLNLGDEKPYRSSAVYAAALHSSTIPFRMQP--TSSDSSEVSNSMDVNTLV 360

Query: 361 RMLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHHQSQASAICNTGKHLDQPL 420
           ++L+G+GRQN+VAILD AMPAP+L                            GK L+  L
Sbjct: 361 QLLTGRGRQNIVAILDSAMPAPTL---------------------------AGKQLENTL 420

Query: 421 LGNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQICAAYEGSTE--RPM 480
           L +LQ LTPEV ED ED  A+ESM + G        A V E+K+ + A+YE +T   +P+
Sbjct: 421 LTSLQALTPEVTEDVEDNQAVESMCILGALRSEDKEALVSEVKNAVDASYEQATTKGKPL 480

Query: 481 LCHLSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLDVHSIPMAARLRSSK 540
            C+LS ++CPLP+PLPFPSIFGNLVG+ GE+L++P+S +  RGSLDVHSIP+A R RSS 
Sbjct: 481 FCNLSVSRCPLPVPLPFPSIFGNLVGRKGEILSSPVSDSLYRGSLDVHSIPVATRWRSSS 540

Query: 541 AILPFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVSKMVLALNPHSQSSS 600
           AILPFLE R+ NL + G   G+  + ++R WGFGREEL++M E++SKMV  LNP    SS
Sbjct: 541 AILPFLETRMGNLEKLGIQWGAMGSDVVRAWGFGREELQEMRENLSKMVSELNPQFFESS 562

BLAST of MS011414 vs. TAIR 10
Match: AT4G37190.2 (LOCATED IN: cytosol, plasma membrane; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Misato Segment II, myosin-like (InterPro:IPR019605), Tubulin/FtsZ, N-terminal (InterPro:IPR019746); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 594.3 bits (1531), Expect = 1.1e-169
Identity = 316/557 (56.73%), Postives = 402/557 (72.17%), Query Frame = 0

Query: 47  MDVLYRSGETQQGVLTYTPRLVSVGFKGGLGSVSARGTLYNDDAYTPSD-VITWRGNVST 106
           MDVLYRSGETQQGV TYTPRLVSV  KG LG++S+RGTLYN+ + + SD   TW G+V T
Sbjct: 1   MDVLYRSGETQQGVATYTPRLVSVNLKGALGTMSSRGTLYNEGSSSRSDSSATWFGDVDT 60

Query: 107 HHTEPLKKNLFLQSLSEEEQENSMNGKNSEQATSRREIQDKDIVECLEKDVTFWTDFSKV 166
             +EP K+NLFLQSL EEE    + GK        +EI+DKDIV CL+++V  WTDFSK 
Sbjct: 61  QRSEPRKRNLFLQSLYEEEH---VVGKE-----KAKEIEDKDIVGCLDEEVECWTDFSKS 120

Query: 167 HYHPQSLYQLNGLWVDVQEFDNYGIGKESFSWSSQREDIDERLRFFVEECDHIQGFQFIV 226
           HYHPQSLY+LNGLW+D Q F+NYGIGK+ FS +S+ E+I +RLRFFVEECDHIQG +F+V
Sbjct: 121 HYHPQSLYELNGLWMDSQAFNNYGIGKDVFSEASRGEEICDRLRFFVEECDHIQGIKFLV 180

Query: 227 DDSGGFSAIAGDFLENVADEYSNTPVLLYSVRSPGSYSMQRENKRQTVSRNLHDAVSFAR 286
           DDSGGFSA+A DFLEN+ADEY+N PVLLYSVR+P    M + + ++TVS  LHDA+SF+R
Sbjct: 181 DDSGGFSAVAADFLENMADEYTNVPVLLYSVRTP----MSQMSSKKTVSNKLHDAISFSR 240

Query: 287 LSSFCQLFMPVGLPSLSRSKAITHLCIDDQKPYHCSAVYAAALHSIGLPLRMEAHGPTAD 346
           LSSFC+LF P+GLPSL+ SKA   L + D+KPY  SAVYAAALHS  +P RM+    ++D
Sbjct: 241 LSSFCKLFTPIGLPSLTGSKASKFLNLGDEKPYRSSAVYAAALHSSTIPFRMQP--TSSD 300

Query: 347 SCYVSGAVHINDMVRMLSGQGRQNMVAILDVAMPAPSLCGIYSSIFFYLQKRTFHHQSQA 406
           S  VS ++ +N +V++L+G+GRQN+VAILD AMPAP+L                      
Sbjct: 301 SSEVSNSMDVNTLVQLLTGRGRQNIVAILDSAMPAPTL---------------------- 360

Query: 407 SAICNTGKHLDQPLLGNLQPLTPEVAEDAEDPLALESMTVHGVFGLGGDRASVPELKDQI 466
                 GK L+  LL +LQ LTPEV ED ED  A+ESM + G        A V E+K+ +
Sbjct: 361 -----AGKQLENTLLTSLQALTPEVTEDVEDNQAVESMCILGALRSEDKEALVSEVKNAV 420

Query: 467 CAAYEGSTE--RPMLCHLSATQCPLPIPLPFPSIFGNLVGQHGELLATPISGASSRGSLD 526
            A+YE +T   +P+ C+LS ++CPLP+PLPFPSIFGNLVG+ GE+L++P+S +  RGSLD
Sbjct: 421 DASYEQATTKGKPLFCNLSVSRCPLPVPLPFPSIFGNLVGRKGEILSSPVSDSLYRGSLD 480

Query: 527 VHSIPMAARLRSSKAILPFLERRLTNLLRHGTAQGSSAASLLRNWGFGREELEDMGESVS 586
           VHSIP+A R RSS AILPFLE R+ NL + G   G+  + ++R WGFGREEL++M E++S
Sbjct: 481 VHSIPVATRWRSSSAILPFLETRMGNLEKLGIQWGAMGSDVVRAWGFGREELQEMRENLS 516

Query: 587 KMVLALNPHSQSSSDSD 601
           KMV  LNP    SSDSD
Sbjct: 541 KMVSELNPQFFESSDSD 516