MS011365 (gene) Bitter gourd (TR) v1

Overview


Name	MS011365
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	E3 ubiquitin-protein ligase RNF185
Location	scaffold239: 1346208 .. 1346891 (+)
RNA-Seq Expression	MS011365
Synteny	MS011365

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAACGAGTTTGGAGAATCAACAAGCGTGCCACATCAAAACCCCTTTTGTTCCATTAACAGTGACAGCAGCAGCAATGACACTGGTGATTTCGAATGCAATATTTGCTTCGAGTTAGCTCAAGATCCAATCATCACTCTTTGCGGCCATCTCTATTGCTGGCCCTGTCTGTATAGATGGCTGCACTATCACTCGCATTCCCAAGAATGTCCAGTTTGTAAGGCTGTTGTTCAGGAGGAGAAGTTGGTTCCCCTTTATGGTAGGGGAAAGATACCATCTGACCCAAGATTAACTAGATATCCCGGTATTGACGTTCCCAATCGCCCTGCTGGGCAAAGGCCTCAAACTGCACCTCCCCCTGTTGCCAATGACTTTGCAAACTACGGGTTTGGATTCACTGGTGGGTTTATGCCAATGGCAACAGCAAGGATTGGCAACTTCACTTTGGCTACTGCTTTTGGTGGGCTGATCCCATCTTTATTCAACATACAGTTTCATGGATTTCCTGATGCTACAGTTTATGGAACTACTTCTGGTTATCCCTATGCTTTCAATACATTCCATGGGGGTCATGGCCACCATTTTCCTCAACCGCCAATTCGAGGTCAGCATGCTGATAACGTACTGAAAAACCTCCTTTTGCTTGTTGGTGTCTTTGTAATTCTTGCCTTGCTTTGGTGG

mRNA sequence

ATGGCGAACGAGTTTGGAGAATCAACAAGCGTGCCACATCAAAACCCCTTTTGTTCCATTAACAGTGACAGCAGCAGCAATGACACTGGTGATTTCGAATGCAATATTTGCTTCGAGTTAGCTCAAGATCCAATCATCACTCTTTGCGGCCATCTCTATTGCTGGCCCTGTCTGTATAGATGGCTGCACTATCACTCGCATTCCCAAGAATGTCCAGTTTGTAAGGCTGTTGTTCAGGAGGAGAAGTTGGTTCCCCTTTATGGTAGGGGAAAGATACCATCTGACCCAAGATTAACTAGATATCCCGGTATTGACGTTCCCAATCGCCCTGCTGGGCAAAGGCCTCAAACTGCACCTCCCCCTGTTGCCAATGACTTTGCAAACTACGGGTTTGGATTCACTGGTGGGTTTATGCCAATGGCAACAGCAAGGATTGGCAACTTCACTTTGGCTACTGCTTTTGGTGGGCTGATCCCATCTTTATTCAACATACAGTTTCATGGATTTCCTGATGCTACAGTTTATGGAACTACTTCTGGTTATCCCTATGCTTTCAATACATTCCATGGGGGTCATGGCCACCATTTTCCTCAACCGCCAATTCGAGGTCAGCATGCTGATAACGTACTGAAAAACCTCCTTTTGCTTGTTGGTGTCTTTGTAATTCTTGCCTTGCTTTGGTGG

Coding sequence (CDS)

ATGGCGAACGAGTTTGGAGAATCAACAAGCGTGCCACATCAAAACCCCTTTTGTTCCATTAACAGTGACAGCAGCAGCAATGACACTGGTGATTTCGAATGCAATATTTGCTTCGAGTTAGCTCAAGATCCAATCATCACTCTTTGCGGCCATCTCTATTGCTGGCCCTGTCTGTATAGATGGCTGCACTATCACTCGCATTCCCAAGAATGTCCAGTTTGTAAGGCTGTTGTTCAGGAGGAGAAGTTGGTTCCCCTTTATGGTAGGGGAAAGATACCATCTGACCCAAGATTAACTAGATATCCCGGTATTGACGTTCCCAATCGCCCTGCTGGGCAAAGGCCTCAAACTGCACCTCCCCCTGTTGCCAATGACTTTGCAAACTACGGGTTTGGATTCACTGGTGGGTTTATGCCAATGGCAACAGCAAGGATTGGCAACTTCACTTTGGCTACTGCTTTTGGTGGGCTGATCCCATCTTTATTCAACATACAGTTTCATGGATTTCCTGATGCTACAGTTTATGGAACTACTTCTGGTTATCCCTATGCTTTCAATACATTCCATGGGGGTCATGGCCACCATTTTCCTCAACCGCCAATTCGAGGTCAGCATGCTGATAACGTACTGAAAAACCTCCTTTTGCTTGTTGGTGTCTTTGTAATTCTTGCCTTGCTTTGGTGG

Protein sequence

MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYRWLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPPPVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW

Homology

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BLAST of MS011365 vs. NCBI nr
Match: XP_022132409.1 (E3 ubiquitin-protein ligase RNF185 [Momordica charantia] >XP_022132410.1 E3 ubiquitin-protein ligase RNF185 [Momordica charantia])

HSP 1 Score: 490.0 bits (1260), Expect = 1.1e-134
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 1   MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR 60
           MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR
Sbjct: 1   MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR 60

Query: 61  WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP 120
           WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP
Sbjct: 61  WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW 228

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BLAST of MS011365 vs. NCBI nr
Match: TYK12950.1 (E3 ubiquitin-protein ligase RNF185 [Cucumis melo var. makuwa])

HSP 1 Score: 458.4 bits (1178), Expect = 3.7e-125
Identity = 210/228 (92.11%), Postives = 220/228 (96.49%), Query Frame = 0


Query: 1   MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR 60
           MANEFGESTS+PH+NP CS NSD+++NDTGDFECNICFELAQDPIITLCGHL+CWPCLYR
Sbjct: 1   MANEFGESTSMPHRNPSCSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP 120
           WLH+HS  QECPVCKA+VQEEKLVPLYGRGKIPSDPRL RYPGID+PNRPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKIPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PV NDFANYGFGFTGGFMPMA+ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP  RGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPSTRGQHADNVLKNLLLLVGVFVILALLWW 228

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BLAST of MS011365 vs. NCBI nr
Match: XP_008440158.1 (PREDICTED: E3 ubiquitin-protein ligase RNF185 [Cucumis melo] >XP_008440160.1 PREDICTED: E3 ubiquitin-protein ligase RNF185 [Cucumis melo])

HSP 1 Score: 458.0 bits (1177), Expect = 4.8e-125
Identity = 209/228 (91.67%), Postives = 220/228 (96.49%), Query Frame = 0


Query: 1   MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR 60
           MANEFGESTS+PH+NP CS NSD+++NDTGDFECNICFELAQDPIITLCGHL+CWPCLYR
Sbjct: 1   MANEFGESTSMPHRNPSCSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP 120
           WLH+HS  QECPVCKA++QEEKLVPLYGRGKIPSDPRL RYPGID+PNRPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PV NDFANYGFGFTGGFMPMA+ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP  RGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPSTRGQHADNVLKNLLLLVGVFVILALLWW 228

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BLAST of MS011365 vs. NCBI nr
Match: XP_022949606.1 (E3 ubiquitin-protein ligase RNF185-like [Cucurbita moschata] >XP_022978947.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita maxima] >XP_023543222.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita pepo subsp. pepo] >KAG7034387.1 RING finger protein 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 455.7 bits (1171), Expect = 2.4e-124
Identity = 210/228 (92.11%), Postives = 217/228 (95.18%), Query Frame = 0


Query: 1   MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR 60
           MANEFGESTSVPHQNP  S NSD+++NDTGDFECNICFELAQDPIITLCGHL+CWPCLYR
Sbjct: 1   MANEFGESTSVPHQNPSWSSNSDNTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP 120
           WLH+HS  QECPVCKA+VQEEKLVPLYGRGK PSDPRL RYPGID+PNRPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKAPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           P ANDFANYGFGFTGGFMPMA ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PAANDFANYGFGFTGGFMPMAAARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQPP RGQHADNVLKNL LLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPPSRGQHADNVLKNLFLLVGVFVILALLWW 228

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BLAST of MS011365 vs. NCBI nr
Match: KAG6604228.1 (E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 455.7 bits (1171), Expect = 2.4e-124
Identity = 210/228 (92.11%), Postives = 217/228 (95.18%), Query Frame = 0


Query: 1   MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR 60
           MANEFGESTSVPHQNP  S NSD+++NDTGDFECNICFELAQDPIITLCGHL+CWPCLYR
Sbjct: 59  MANEFGESTSVPHQNPSWSSNSDNTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 118

Query: 61  WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP 120
           WLH+HS  QECPVCKA+VQEEKLVPLYGRGK PSDPRL RYPGID+PNRPAGQRPQTAPP
Sbjct: 119 WLHHHSQCQECPVCKALVQEEKLVPLYGRGKAPSDPRLNRYPGIDIPNRPAGQRPQTAPP 178

Query: 121 PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           P ANDFANYGFGFTGGFMPMA ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 179 PAANDFANYGFGFTGGFMPMAAARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 238

Query: 181 YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQPP RGQHADNVLKNL LLVGVFVILALLWW
Sbjct: 239 YPYAFNTFHGGHGHHFPQPPSRGQHADNVLKNLFLLVGVFVILALLWW 286

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BLAST of MS011365 vs. ExPASy Swiss-Prot
Match: Q09463 (E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 6.3e-27
Identity = 83/245 (33.88%), Postives = 116/245 (47.35%), Query Frame = 0


Query: 9   TSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYRWLHYHSHS 68
           T  P + P  S N D S+     FECNIC + A+D +++LCGHL+CWPCL +WL    ++
Sbjct: 5   TKAPSEEPTSSSNKDESAR----FECNICLDAAKDAVVSLCGHLFCWPCLSQWLDTRPNN 64

Query: 69  QECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPPPVANDFAN 128
           Q CPVCK+ +   K+VP+YGRG   SDPR        VP RP GQR +  PPP    FA 
Sbjct: 65  QVCPVCKSAIDGNKVVPIYGRGGDSSDPRK------KVPPRPKGQRSE--PPP--QSFAG 124

Query: 129 YGFGFTGGFMPMATARIG-NFTLATAFGGLIPSLFNIQF---------------HGFPDA 188
           + +G  GG M      +  +F + T   GL P +F + F               +G   A
Sbjct: 125 FNWGGDGGMMGGGGPNVHFSFGIGTV-NGLFPLMFMLPFIQGIFPLSFVASFFGNGNQGA 184

Query: 189 TVYGTTSG---------YPYAFNTFHGGHGH-HFPQPPIRGQHADNVLKNLLLLVGVFVI 228
              G  +G         + +     HG  GH     P  R    +  L N+   +G F++
Sbjct: 185 AAAGGGNGGGNDGNDGTHAHTHGHTHGPRGHGESAAPGSRMAQEEEYLSNIFKYIGFFML 234

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BLAST of MS011365 vs. ExPASy Swiss-Prot
Match: Q5ZIR9 (E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.5e-25
Identity = 71/165 (43.03%), Postives = 89/165 (53.94%), Query Frame = 0


Query: 21  NSDSSSNDTGD-------FECNICFELAQDPIITLCGHLYCWPCLYRWLHYHSHSQECPV 80
           +  SSSN  GD       FECNIC + A+D +I+LCGHL+CWPCL++WL    + Q CPV
Sbjct: 21  SGSSSSNGAGDNTNQDNTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPV 80

Query: 81  CKAVVQEEKLVPLYGRGKI-PSDPRLTRYPGIDVPNRPAGQRPQTAPPPVANDFANYGFG 140
           CKA +  +K++PLYGRG     DPR         P RP GQRP+   P     F  +GFG
Sbjct: 81  CKAGISRDKVIPLYGRGSTGQQDPR------EKTPPRPQGQRPE---PENRGGFQGFGFG 140

Query: 141 FTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGT 178
             GGF    +  IG F       G+  + FNI   G P   V GT
Sbjct: 141 -DGGF--QMSFGIGAFPF-----GIFATAFNIN-DGRPPPAVPGT 167

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BLAST of MS011365 vs. ExPASy Swiss-Prot
Match: Q568Y3 (E3 ubiquitin-protein ligase RNF185 OS=Rattus norvegicus OX=10116 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.7e-24
Identity = 74/180 (41.11%), Postives = 93/180 (51.67%), Query Frame = 0


Query: 6   GESTSVPHQNPFCSINSDSSSNDTGD-------FECNICFELAQDPIITLCGHLYCWPCL 65
           G S S   +N         SSN TG+       FECNIC + A+D +I+LCGHL+CWPCL
Sbjct: 5   GPSASASTENSSAG-GPSGSSNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCL 64

Query: 66  YRWLHYHSHSQECPVCKAVVQEEKLVPLYGRGKI-PSDPRLTRYPGIDVPNRPAGQRPQT 125
           ++WL    + Q CPVCKA +  +K++PLYGRG     DPR         P RP GQRP+ 
Sbjct: 65  HQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPR------EKTPPRPQGQRPE- 124

Query: 126 APPPVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGT 178
             P     F  +GFG  GGF    +  IG F       G+  + FNI   G P   V GT
Sbjct: 125 --PENRGGFQGFGFG-DGGF--QMSFGIGAFPF-----GIFATAFNIN-DGRPPPAVPGT 165

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BLAST of MS011365 vs. ExPASy Swiss-Prot
Match: Q91YT2 (E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.2e-24
Identity = 74/180 (41.11%), Postives = 93/180 (51.67%), Query Frame = 0


Query: 6   GESTSVPHQNPFCSINSDSSSNDTGD-------FECNICFELAQDPIITLCGHLYCWPCL 65
           G S S   +N         SSN TG+       FECNIC + A+D +I+LCGHL+CWPCL
Sbjct: 5   GPSASASTENSNAG-GPSGSSNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCL 64

Query: 66  YRWLHYHSHSQECPVCKAVVQEEKLVPLYGRGKI-PSDPRLTRYPGIDVPNRPAGQRPQT 125
           ++WL    + Q CPVCKA +  +K++PLYGRG     DPR         P RP GQRP+ 
Sbjct: 65  HQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPR------EKTPPRPQGQRPE- 124

Query: 126 APPPVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGT 178
             P     F  +GFG  GGF    +  IG F       G+  + FNI   G P   V GT
Sbjct: 125 --PENRGGFQGFGFG-DGGF--QMSFGIGAFPF-----GIFATAFNIN-DGRPPPAVPGT 165

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BLAST of MS011365 vs. ExPASy Swiss-Prot
Match: Q99942 (E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens OX=9606 GN=RNF5 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.8e-24
Identity = 57/107 (53.27%), Postives = 68/107 (63.55%), Query Frame = 0


Query: 32  FECNICFELAQDPIITLCGHLYCWPCLYRWLHYHSHSQECPVCKAVVQEEKLVPLYGRG- 91
           FECNIC E A++ ++++CGHLYCWPCL++WL      QECPVCKA +  EK+VPLYGRG 
Sbjct: 25  FECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGS 84

Query: 92  KIPSDPRLTRYPGIDVPNRPAGQRPQTAPPPVANDFANYGFGFTGGF 138
           + P DPRL        P RP GQRP    P     F    FG TGGF
Sbjct: 85  QKPQDPRL------KTPPRPQGQRP---APESRGGF--QPFGDTGGF 120

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BLAST of MS011365 vs. ExPASy TrEMBL
Match: A0A6J1BSE2 (E3 ubiquitin-protein ligase RNF185 OS=Momordica charantia OX=3673 GN=LOC111005272 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 5.5e-135
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 1   MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR 60
           MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR
Sbjct: 1   MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR 60

Query: 61  WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP 120
           WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP
Sbjct: 61  WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW 228

[Close]

BLAST of MS011365 vs. ExPASy TrEMBL
Match: A0A5D3CNC7 (E3 ubiquitin-protein ligase RNF185 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005460 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.8e-125
Identity = 210/228 (92.11%), Postives = 220/228 (96.49%), Query Frame = 0


Query: 1   MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR 60
           MANEFGESTS+PH+NP CS NSD+++NDTGDFECNICFELAQDPIITLCGHL+CWPCLYR
Sbjct: 1   MANEFGESTSMPHRNPSCSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP 120
           WLH+HS  QECPVCKA+VQEEKLVPLYGRGKIPSDPRL RYPGID+PNRPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKIPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PV NDFANYGFGFTGGFMPMA+ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP  RGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPSTRGQHADNVLKNLLLLVGVFVILALLWW 228

[Close]

BLAST of MS011365 vs. ExPASy TrEMBL
Match: A0A1S3B021 (E3 ubiquitin-protein ligase RNF185 OS=Cucumis melo OX=3656 GN=LOC103484707 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 2.3e-125
Identity = 209/228 (91.67%), Postives = 220/228 (96.49%), Query Frame = 0


Query: 1   MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR 60
           MANEFGESTS+PH+NP CS NSD+++NDTGDFECNICFELAQDPIITLCGHL+CWPCLYR
Sbjct: 1   MANEFGESTSMPHRNPSCSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP 120
           WLH+HS  QECPVCKA++QEEKLVPLYGRGKIPSDPRL RYPGID+PNRPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PV NDFANYGFGFTGGFMPMA+ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP  RGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPSTRGQHADNVLKNLLLLVGVFVILALLWW 228

[Close]

BLAST of MS011365 vs. ExPASy TrEMBL
Match: A0A6J1IMG5 (E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita maxima OX=3661 GN=LOC111478748 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.2e-124
Identity = 210/228 (92.11%), Postives = 217/228 (95.18%), Query Frame = 0


Query: 1   MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR 60
           MANEFGESTSVPHQNP  S NSD+++NDTGDFECNICFELAQDPIITLCGHL+CWPCLYR
Sbjct: 1   MANEFGESTSVPHQNPSWSSNSDNTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP 120
           WLH+HS  QECPVCKA+VQEEKLVPLYGRGK PSDPRL RYPGID+PNRPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKAPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           P ANDFANYGFGFTGGFMPMA ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PAANDFANYGFGFTGGFMPMAAARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQPP RGQHADNVLKNL LLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPPSRGQHADNVLKNLFLLVGVFVILALLWW 228

[Close]

BLAST of MS011365 vs. ExPASy TrEMBL
Match: A0A6J1GCG3 (E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita moschata OX=3662 GN=LOC111452948 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.2e-124
Identity = 210/228 (92.11%), Postives = 217/228 (95.18%), Query Frame = 0


Query: 1   MANEFGESTSVPHQNPFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYR 60
           MANEFGESTSVPHQNP  S NSD+++NDTGDFECNICFELAQDPIITLCGHL+CWPCLYR
Sbjct: 1   MANEFGESTSVPHQNPSWSSNSDNTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHYHSHSQECPVCKAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPP 120
           WLH+HS  QECPVCKA+VQEEKLVPLYGRGK PSDPRL RYPGID+PNRPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKAPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           P ANDFANYGFGFTGGFMPMA ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PAANDFANYGFGFTGGFMPMAAARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPPIRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQPP RGQHADNVLKNL LLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPPSRGQHADNVLKNLFLLVGVFVILALLWW 228

[Close]

BLAST of MS011365 vs. TAIR 10
Match: AT2G23780.1 (RING/U-box superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 1.4e-74
Identity = 139/227 (61.23%), Postives = 165/227 (72.69%), Query Frame = 0


Query: 19  SINSDSSSNDT----GDFECNICFELAQDPIITLCGHLYCWPCLYRWLHYHSHSQECPVC 78
           S +   S+NDT    GDFECNICFELAQDPI+TLCGHL+CWPCLYRWLH+HSHSQECPVC
Sbjct: 9   STSYSDSNNDTNDQGGDFECNICFELAQDPIVTLCGHLFCWPCLYRWLHHHSHSQECPVC 68

Query: 79  KAVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPPP----VANDFANYG 138
           KAVVQ++KLVPLYGRGK  +DPR  RYPG+ +PNRP GQRP+TA PP     A++F NYG
Sbjct: 69  KAVVQDDKLVPLYGRGKNQTDPRSKRYPGLRIPNRPTGQRPETAAPPPQPEAASNFFNYG 128

Query: 139 FGFTGGFMP-MATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPYAFNTFH 198
            G  GG MP MAT R GNF++   FGGL+PSLFN QFHGF DAT+YG+T GYPY      
Sbjct: 129 IGLMGGIMPMMATTRFGNFSM--GFGGLLPSLFNFQFHGFHDATLYGSTPGYPY------ 188

Query: 199 GGHGHHFPQPPIRGQ---------HADNVLKNLLLLVGVFVILALLW 228
           GG+ + F   P RGQ          +D  LKN+L  VG+ V++ L+W
Sbjct: 189 GGYHNGFRGVPPRGQERPMARGGNQSDAFLKNILFFVGICVVIFLIW 227

[Close]

BLAST of MS011365 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 3.6e-70
Identity = 130/219 (59.36%), Postives = 167/219 (76.26%), Query Frame = 0


Query: 16  PFCSINSDSSSNDTGDFECNICFELAQDPIITLCGHLYCWPCLYRWLHYHSHSQECPVCK 75
           P CS  +D+++ND+ +FECNIC +LAQDPI+TLCGHL+CWPCLY+WLH HS S++CPVCK
Sbjct: 5   PSCSSGNDTNNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQSKDCPVCK 64

Query: 76  AVVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPPPVAN-DFA-NYGF-G 135
           AV++E++LVPLYGRGK  +DPR    PG++VPNRP+GQRP+TA PP  N  FA ++GF G
Sbjct: 65  AVIEEDRLVPLYGRGKSSADPRSKSIPGLEVPNRPSGQRPETAQPPDPNHGFAHHHGFGG 124

Query: 136 FTGGF-MPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYG--TTSGYPYAF-NTF 195
           F GGF  PMA+AR GN TL+ AFGGLIPSLFN+ FHGFPDA +YG   + G+P+ F N F
Sbjct: 125 FMGGFAAPMASARFGNVTLSAAFGGLIPSLFNLHFHGFPDAAMYGAAASGGFPHGFSNPF 184

Query: 196 HGGHG--HHFPQPPIRGQHADNVLKNLLLLVGVFVILAL 226
           HGGH   H + +   R    D+ L+ LLL+V V V+ +L
Sbjct: 185 HGGHSHMHSYQRHTGRQGQQDHHLRILLLIVFVVVVFSL 223

[Close]

BLAST of MS011365 vs. TAIR 10
Match: AT1G74990.1 (RING/U-box superfamily protein )

HSP 1 Score: 146.0 bits (367), Expect = 3.8e-35
Identity = 74/138 (53.62%), Postives = 94/138 (68.12%), Query Frame = 0


Query: 19  SINSDSSSND--TGDFECNICFELAQDPIITLCGHLYCWPCLYRWLHYHSHSQECPVCKA 78
           +IN+ ++  D  + +F CNIC ELA++PI+TLCGHL+CWPCLY+WLHYHS S  CPVCKA
Sbjct: 2   AINTITNEEDDASNNFGCNICLELAREPIVTLCGHLFCWPCLYKWLHYHSKSNHCPVCKA 61

Query: 79  VVQEEKLVPLYGRGKIPSDPRLTRYPGIDVPNRPAGQRPQTAPPPVANDFANYGFGFTGG 138
           +V+E+ LVPLYG GK  SDPR     G+ VPNRPA  R +TA P +  +  ++G  F GG
Sbjct: 62  LVKEDTLVPLYGMGKPSSDPRSKLNSGVTVPNRPAATRTETARPRL--EQRHHGSSFFGG 121

Query: 139 ---FMPMATA-RIGNFTL 151
              F  M T  R  NF L
Sbjct: 122 HSSFAAMPTGLRFSNFLL 137

[Close]

BLAST of MS011365 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 100.1 bits (248), Expect = 2.4e-21
Identity = 46/99 (46.46%), Postives = 59/99 (59.60%), Query Frame = 0


Query: 26  SNDTGDFECNICFELAQDPIITLCGHLYCWPCLYRWLHYH-------SHSQECPVCKAVV 85
           +N++G F+CNIC + A DP++TLCGHL+CWPC+Y+WLH          H   CPVCK+ +
Sbjct: 36  ANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNI 95

Query: 86  QEEKLVPLYGRGKIPSDPRLT------RYPGIDVPNRPA 112
               LVPLYGRG   S P  T           D+P RPA
Sbjct: 96  TITSLVPLYGRGM--SSPSSTFGSKKQDALSTDIPRRPA 132

[Close]

BLAST of MS011365 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 96.7 bits (239), Expect = 2.6e-20
Identity = 43/97 (44.33%), Postives = 61/97 (62.89%), Query Frame = 0


Query: 30  GDFECNICFELAQDPIITLCGHLYCWPCLYRWLHYHSHSQE-------------CPVCKA 89
           GDF+CNIC +  +DP++TLCGHL+CWPC+++W +  ++S++             CPVCK+
Sbjct: 17  GDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKS 76

Query: 90  VVQEEKLVPLYGRG-KIPSDPRLTRYPGIDVPNRPAG 113
            V E  LVP+YGRG K P         G +VP+RP G
Sbjct: 77  DVSEATLVPIYGRGQKAPQS-------GSNVPSRPTG 106

The following BLAST results are available for this feature:

BLAST of MS011365 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132409.1	1.1e-134	100.00	E3 ubiquitin-protein ligase RNF185 [Momordica charantia] >XP_022132410.1 E3 ubiq...	[more]
TYK12950.1	3.7e-125	92.11	E3 ubiquitin-protein ligase RNF185 [Cucumis melo var. makuwa]	[more]
XP_008440158.1	4.8e-125	91.67	PREDICTED: E3 ubiquitin-protein ligase RNF185 [Cucumis melo] >XP_008440160.1 PRE...	[more]
XP_022949606.1	2.4e-124	92.11	E3 ubiquitin-protein ligase RNF185-like [Cucurbita moschata] >XP_022978947.1 E3 ...	[more]
KAG6604228.1	2.4e-124	92.11	E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MS011365 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q09463	6.3e-27	33.88	E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1	[more]
Q5ZIR9	4.5e-25	43.03	E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1	[more]
Q568Y3	1.7e-24	41.11	E3 ubiquitin-protein ligase RNF185 OS=Rattus norvegicus OX=10116 GN=Rnf185 PE=2 ...	[more]
Q91YT2	2.2e-24	41.11	E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1	[more]
Q99942	3.8e-24	53.27	E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens OX=9606 GN=RNF5 PE=1 SV=1	[more]

BLAST of MS011365 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BSE2	5.5e-135	100.00	E3 ubiquitin-protein ligase RNF185 OS=Momordica charantia OX=3673 GN=LOC11100527...	[more]
A0A5D3CNC7	1.8e-125	92.11	E3 ubiquitin-protein ligase RNF185 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B021	2.3e-125	91.67	E3 ubiquitin-protein ligase RNF185 OS=Cucumis melo OX=3656 GN=LOC103484707 PE=4 ...	[more]
A0A6J1IMG5	1.2e-124	92.11	E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita maxima OX=3661 GN=LOC111478...	[more]
A0A6J1GCG3	1.2e-124	92.11	E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of MS011365 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23780.1	1.4e-74	61.23	RING/U-box superfamily protein	[more]
AT1G19310.1	3.6e-70	59.36	RING/U-box superfamily protein	[more]
AT1G74990.1	3.8e-35	53.62	RING/U-box superfamily protein	[more]
AT4G27470.1	2.4e-21	46.46	RING membrane-anchor 3	[more]
AT4G28270.1	2.6e-20	44.33	RING membrane-anchor 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 34..74 e-value: 1.5E-7 score: 41.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 34..75 score: 13.167916
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 19..91 e-value: 1.5E-17 score: 65.2
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 32..79 e-value: 8.3E-9 score: 35.2
None	No IPR available	PANTHER	PTHR12313:SF47	RING ZINC FINGER PROTEIN-RELATED	coord: 2..227
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 32..76 e-value: 1.40621E-29 score: 102.768
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 29..87
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 2..227
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 49..58

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS011365.1	MS011365.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
molecular_function	GO:0061630	ubiquitin protein ligase activity