MS011273 (gene) Bitter gourd (TR) v1

BLAST of MS011273 vs. NCBI nr
Match: XP_022132703.1 (cysteine proteinase inhibitor 5-like [Momordica charantia])

HSP 1 Score: 236.9 bits (603), Expect = 9.2e-59
Identity = 120/121 (99.17%), Postives = 120/121 (99.17%), Query Frame = 0

Query: 1   MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV 60
           MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV
Sbjct: 32  MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV 91

Query: 61  VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKSSDKSWKVLSFAPL 120
           VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDK SDKSWKVLSFAPL
Sbjct: 92  VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDKSWKVLSFAPL 151

Query: 121 E 122
           E
Sbjct: 152 E 152

BLAST of MS011273 vs. NCBI nr
Match: XP_022132702.1 (cysteine proteinase inhibitor 5-like [Momordica charantia])

HSP 1 Score: 216.5 bits (550), Expect = 1.3e-52
Identity = 111/122 (90.98%), Postives = 115/122 (94.26%), Query Frame = 0

Query: 1   MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQ-PKPVGLVASMEFVG 60
           MDSVAVAPAQNDAL H+K PGGWEPIKNIKDPYVQEMGRFAVMEQ PKP  L+ASMEF+G
Sbjct: 1   MDSVAVAPAQNDALLHLKMPGGWEPIKNIKDPYVQEMGRFAVMEQHPKPSELIASMEFIG 60

Query: 61  VVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKSSDKSWKVLSFAP 120
           VVSGEIQLVA GKEYRLVVEV+VTEKIIVHGCPVSSIRLYVAVVLDK SDKSWKVLSFAP
Sbjct: 61  VVSGEIQLVAHGKEYRLVVEVRVTEKIIVHGCPVSSIRLYVAVVLDKPSDKSWKVLSFAP 120

Query: 121 LE 122
           LE
Sbjct: 121 LE 122

BLAST of MS011273 vs. NCBI nr
Match: XP_022132557.1 (cysteine proteinase inhibitor 6-like [Momordica charantia])

HSP 1 Score: 129.0 bits (323), Expect = 2.7e-26
Identity = 76/123 (61.79%), Postives = 92/123 (74.80%), Query Frame = 0

Query: 1   MDSVAVAPA--QNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFV 60
           MD VAVAPA  QNDAL +V+KPGGWEPIKNI DPYVQE+GRFAVME    +   AS EF+
Sbjct: 1   MDPVAVAPAVQQNDALLNVQKPGGWEPIKNINDPYVQELGRFAVMEHTHNL-CGASYEFI 60

Query: 61  GVVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKSSDKSWKVLSFA 120
            VVSGE Q+V  GK+Y LV+EVK  E II+   P SSI+ + A++LDK+ +KSW +LSF 
Sbjct: 61  RVVSGESQVV-GGKKYCLVIEVK--ETIIIPSMP-SSIKFFKAIMLDKAWEKSWALLSFV 118

Query: 121 PLE 122
           P E
Sbjct: 121 PCE 118

BLAST of MS011273 vs. NCBI nr
Match: WP_198843152.1 (hypothetical protein, partial [Aureibaculum sp. A20] >MBJ2176574.1 hypothetical protein [Aureibaculum sp. A20])

HSP 1 Score: 115.9 bits (289), Expect = 2.4e-22
Identity = 71/122 (58.20%), Postives = 87/122 (71.31%), Query Frame = 0

Query: 1   MDSVAVAPAQNDAL-QHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQP-KPVGLVASMEFV 60
           MD   +AP QNDAL Q+  KPGGWEPIKNI DPYVQEMGRF+VME   K VG  ASM+F+
Sbjct: 5   MDFEVIAPGQNDALHQNRCKPGGWEPIKNIHDPYVQEMGRFSVMEHTHKLVG--ASMKFI 64

Query: 61  GVVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVS---SIRLYVAVVLDKSSDKSWKVL 118
            VVSGE + ++ GKEYRLVVE  V E II   CP     S++ Y+A VL+K  D+SW++ 
Sbjct: 65  RVVSGETRPMSEGKEYRLVVE--VAEHIIT--CPPMLSVSVKFYLATVLEKPLDRSWQLE 120

BLAST of MS011273 vs. NCBI nr
Match: XP_038882290.1 (cysteine proteinase inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 115.2 bits (287), Expect = 4.1e-22
Identity = 71/120 (59.17%), Postives = 88/120 (73.33%), Query Frame = 0

Query: 1   MDSVAVAPAQNDA-LQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQP-KPVGLVASMEFV 60
           MD   VAP QNDA LQ+  +PG WEPIKNI DPYVQEMGRFAVME   K VG  AS++F+
Sbjct: 1   MDYEVVAPGQNDALLQNRCEPGCWEPIKNIHDPYVQEMGRFAVMEHTHKLVG--ASLKFI 60

Query: 61  GVVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVS-SIRLYVAVVLDKSSDKSWKVLSF 118
            VVSGE + ++ GKEYRLVVE  V E+II     +S SI+ Y+A VL+K  D+SW+++SF
Sbjct: 61  CVVSGETRPMSQGKEYRLVVE--VAEQIITSPPMLSVSIKFYLATVLEKPIDRSWQLISF 116

BLAST of MS011273 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 8.5e-07
Identity = 41/99 (41.41%), Postives = 52/99 (52.53%), Query Frame = 0

Query: 21  GGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGVVSGEIQLVAAGKEYRLVVEV 80
           GGW PI N+ DP V E+G FAV E  K     + ++F  VVSGE Q+V +G  YRL V  
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNK--RSESGLKFETVVSGETQVV-SGTNYRLKVAA 88

Query: 81  KVTEKIIVHGCPVSSIRLYVAVVLDKSSDKSWKVLSFAP 120
                    G  VS  + Y+A+V DK   K   + SF P
Sbjct: 89  N-------DGDGVS--KNYLAIVWDKPWMKFRNLTSFEP 115

BLAST of MS011273 vs. ExPASy Swiss-Prot
Match: Q10Q46 (Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210200 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 7.2e-06
Identity = 37/98 (37.76%), Postives = 52/98 (53.06%), Query Frame = 0

Query: 20  PGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGVVSGEIQLVAAGKEYRLVVE 79
           P GW PIKNI DP++QE+GR+A+ E  + V     + F  V  GE Q V +G  YRL +E
Sbjct: 20  PRGWSPIKNIDDPHIQELGRWAITENNR-VSPSDELTFHRVTGGE-QQVVSGMNYRLEIE 79

Query: 80  VKVTEKIIVHGCPVSSIRLYVAVVLDKSSDKSWKVLSF 118
                     G  V+    Y AVV ++    + K++SF
Sbjct: 80  A------ASGGGDVTG--SYGAVVFEQEWSNTRKLISF 107

BLAST of MS011273 vs. ExPASy Swiss-Prot
Match: Q10J94 (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 7.2e-06
Identity = 39/117 (33.33%), Postives = 53/117 (45.30%), Query Frame = 0

Query: 3   SVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGVVS 62
           S A A        H    GGW PI ++ DP++QE+G +AV            + F  V S
Sbjct: 15  SAAAAAQVGGNRGHGPLVGGWSPITDVGDPHIQELGGWAVERHASLSS--DGLRFRRVTS 74

Query: 63  GEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKSSDKSWKVLSFAP 120
           GE Q V +G  YRLVV             P  +   YVAVV ++S   + ++ SF P
Sbjct: 75  GE-QQVVSGMNYRLVVSA---------SDPAGATASYVAVVYEQSWTNTRQLTSFKP 119

BLAST of MS011273 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 8.0e-05
Identity = 28/59 (47.46%), Postives = 38/59 (64.41%), Query Frame = 0

Query: 21 GGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGVVSGEIQLVAAGKEYRLVVE 80
          G WEP+ +I DP+VQE+GR+AV E+ + V     + F  V  GE Q+V AG  YRL +E
Sbjct: 28 GSWEPV-DINDPHVQELGRWAVAEEDRGVA-AGGLTFERVTDGEKQVV-AGVNYRLTLE 83

BLAST of MS011273 vs. ExPASy TrEMBL
Match: A0A6J1BX23 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005501 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 4.5e-59
Identity = 120/121 (99.17%), Postives = 120/121 (99.17%), Query Frame = 0

Query: 1   MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV 60
           MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV
Sbjct: 32  MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV 91

Query: 61  VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKSSDKSWKVLSFAPL 120
           VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDK SDKSWKVLSFAPL
Sbjct: 92  VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDKSWKVLSFAPL 151

Query: 121 E 122
           E
Sbjct: 152 E 152

BLAST of MS011273 vs. ExPASy TrEMBL
Match: A0A6J1BUK6 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005500 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 6.3e-53
Identity = 111/122 (90.98%), Postives = 115/122 (94.26%), Query Frame = 0

Query: 1   MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQ-PKPVGLVASMEFVG 60
           MDSVAVAPAQNDAL H+K PGGWEPIKNIKDPYVQEMGRFAVMEQ PKP  L+ASMEF+G
Sbjct: 1   MDSVAVAPAQNDALLHLKMPGGWEPIKNIKDPYVQEMGRFAVMEQHPKPSELIASMEFIG 60

Query: 61  VVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKSSDKSWKVLSFAP 120
           VVSGEIQLVA GKEYRLVVEV+VTEKIIVHGCPVSSIRLYVAVVLDK SDKSWKVLSFAP
Sbjct: 61  VVSGEIQLVAHGKEYRLVVEVRVTEKIIVHGCPVSSIRLYVAVVLDKPSDKSWKVLSFAP 120

Query: 121 LE 122
           LE
Sbjct: 121 LE 122

BLAST of MS011273 vs. ExPASy TrEMBL
Match: A0A6J1BU61 (cysteine proteinase inhibitor 6-like OS=Momordica charantia OX=3673 GN=LOC111005388 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.3e-26
Identity = 76/123 (61.79%), Postives = 92/123 (74.80%), Query Frame = 0

Query: 1   MDSVAVAPA--QNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFV 60
           MD VAVAPA  QNDAL +V+KPGGWEPIKNI DPYVQE+GRFAVME    +   AS EF+
Sbjct: 1   MDPVAVAPAVQQNDALLNVQKPGGWEPIKNINDPYVQELGRFAVMEHTHNL-CGASYEFI 60

Query: 61  GVVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKSSDKSWKVLSFA 120
            VVSGE Q+V  GK+Y LV+EVK  E II+   P SSI+ + A++LDK+ +KSW +LSF 
Sbjct: 61  RVVSGESQVV-GGKKYCLVIEVK--ETIIIPSMP-SSIKFFKAIMLDKAWEKSWALLSFV 118

Query: 121 PLE 122
           P E
Sbjct: 121 PCE 118

BLAST of MS011273 vs. ExPASy TrEMBL
Match: A0A6J1GCT2 (uncharacterized protein LOC111453027 OS=Cucurbita moschata OX=3662 GN=LOC111453027 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.0e-19
Identity = 64/120 (53.33%), Postives = 78/120 (65.00%), Query Frame = 0

Query: 1   MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLV-ASMEFVG 60
           MD   VA  QNDAL H  K   WEP+ N+ D YVQE+GRFAVME     GL    M F+ 
Sbjct: 1   MDLKVVAHGQNDALLHKPKSCDWEPV-NVHDQYVQELGRFAVMEHVH--GLCGVGMTFIC 60

Query: 61  VVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKSSDKSWKVLSFAP 120
           VVSGE ++V  GK+Y LVVEVK    ++ H CP  +I+LYVA VLDK   KSW++L+F P
Sbjct: 61  VVSGESKMVCEGKKYSLVVEVKEV-PVLPHACP--AIKLYVADVLDKQCAKSWELLAFKP 114

BLAST of MS011273 vs. ExPASy TrEMBL
Match: A0A6J1FCN5 (cysteine proteinase inhibitor 8-like OS=Cucurbita moschata OX=3662 GN=LOC111442890 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.3e-13
Identity = 53/120 (44.17%), Postives = 76/120 (63.33%), Query Frame = 0

Query: 4   VAVAPAQNDALQHVKK---PGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV 63
           V  AP QN+A+   KK    G W+PI++IKDPYVQEMGRFAVME    + +  +++F+ V
Sbjct: 5   VEAAPGQNEAMFQDKKGIEVGQWKPIEDIKDPYVQEMGRFAVMEHDN-MRIGENLKFICV 64

Query: 64  VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKSSDKSWKVLSFAPL 121
            SGE Q+V  G  YRL+++         +   +SS  LY+A+V DK  +K+WK++ F PL
Sbjct: 65  QSGEKQVV-EGMNYRLLIKAS-------NHLGISS--LYMAIVYDKPWEKTWKLIEFVPL 113

BLAST of MS011273 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 54.7 bits (130), Expect = 6.1e-08
Identity = 41/99 (41.41%), Postives = 52/99 (52.53%), Query Frame = 0

Query: 21  GGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGVVSGEIQLVAAGKEYRLVVEV 80
           GGW PI N+ DP V E+G FAV E  K     + ++F  VVSGE Q+V +G  YRL V  
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNK--RSESGLKFETVVSGETQVV-SGTNYRLKVAA 88

Query: 81  KVTEKIIVHGCPVSSIRLYVAVVLDKSSDKSWKVLSFAP 120
                    G  VS  + Y+A+V DK   K   + SF P
Sbjct: 89  N-------DGDGVS--KNYLAIVWDKPWMKFRNLTSFEP 115