MS011023 (gene) Bitter gourd (TR) v1

BLAST of MS011023 vs. NCBI nr
Match: APO14274.1 (9-cis-epoxycarotenoid dioxygenase NCED2 [Luffa aegyptiaca])

HSP 1 Score: 986.1 bits (2548), Expect = 1.3e-283
Identity = 487/586 (83.11%), Postives = 521/586 (88.91%), Query Frame = 0

Query: 3   SSSSYSSSICNWK-RQRPNNHKRSIIIQSALHSPSVLHFPNQ-NYHNKLSSLREDQDKPI 62
           S+S+ S    N K  Q+ NN    +++  ALHSPSVLHFPNQ NYH KL    +      
Sbjct: 19  SNSTKSIRFKNVKISQQSNNRNAVLVVHCALHSPSVLHFPNQTNYHKKLVLKEDTNTNKP 78

Query: 63  KDP--NWNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNL 122
           K P  NWNF QK A+MAFD+VESAL+S ELQHPLPKTADPRVQISGNFAPVPE PVKHNL
Sbjct: 79  KPPNSNWNFLQKTASMAFDIVESALLSHELQHPLPKTADPRVQISGNFAPVPEQPVKHNL 138

Query: 123 TVAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRL 182
            + GTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAV+ING +ASYACRFTET RL
Sbjct: 139 PITGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVSINGATASYACRFTETQRL 198

Query: 183 VQERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLL 242
           VQER LGR VFPKAIGELHGHSGI RLLLFYGRGLFGLVDHKNGTGVANAGLV+FNDRLL
Sbjct: 199 VQERRLGRSVFPKAIGELHGHSGIARLLLFYGRGLFGLVDHKNGTGVANAGLVYFNDRLL 258

Query: 243 AMSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHK 302
           AMSEDDLPYHVRIT SGDL+TVGRYDF  QL   STMIAHPKVDPVSRELYALSYDVV K
Sbjct: 259 AMSEDDLPYHVRITASGDLETVGRYDFKMQL--KSTMIAHPKVDPVSRELYALSYDVVRK 318

Query: 303 PYLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVI 362
           PYLKYFRFSPDGTKS D+EIPL TPTMMHDFAITENFVVIPDQQVVFKL EMVKGGSPVI
Sbjct: 319 PYLKYFRFSPDGTKSKDLEIPLHTPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVI 378

Query: 363 YDKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSI 422
           YDK+KKSRFGILPKNASD++ +IWVESP+TFCFHLWNAWEEP T+EVVVIGSCMTP DSI
Sbjct: 379 YDKNKKSRFGILPKNASDSNDLIWVESPDTFCFHLWNAWEEPETDEVVVIGSCMTPPDSI 438

Query: 423 FNECDENLKSVLSEIRLNLRTGKSTRRAIMA-EEEQVNLEAGMVNRNRLGRRTRFAYLAI 482
           FNECDENLKSVLSEIRLNLRTGKSTRR I++ EE+QVNLEAGMVNRNRLGR+TR+AYLAI
Sbjct: 439 FNECDENLKSVLSEIRLNLRTGKSTRRPIISDEEQQVNLEAGMVNRNRLGRKTRYAYLAI 498

Query: 483 AEPWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNS---AAEDDGYILAFVHD 542
           AEPWPKVSGFAKVDL TG+VKKHIYG RK+GGEPFFLP++  S     ED+GYILAFVHD
Sbjct: 499 AEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGKSEKEEEEDEGYILAFVHD 558

Query: 543 ERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           ERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVD+N+LQNQA
Sbjct: 559 ERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDANNLQNQA 602

BLAST of MS011023 vs. NCBI nr
Match: XP_038883946.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 980.7 bits (2534), Expect = 5.4e-282
Identity = 489/597 (81.91%), Postives = 525/597 (87.94%), Query Frame = 0

Query: 1   PSSSS-----SYSSSI-CNWKRQRPNNHKRSI--------IIQSALHSPSVLHFPNQ--N 60
           PSSSS     S+S+S   N+     NN+ + I        II SALHSPSVLHFPNQ  N
Sbjct: 8   PSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHFPNQTPN 67

Query: 61  YHNKLSSLREDQDKPIKDPNWNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISG 120
           +HN L+   +DQ       NWN  QK A+MA  +VESAL+S ELQHPLPKTADPRVQISG
Sbjct: 68  FHNNLT--LKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRVQISG 127

Query: 121 NFAPVPEHPVKHNLTVAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGG 180
           NFAPVPE PVKHNL V GTIPDCI+GVYLRNGANPLF+PTAGHHLFDGDGMVHAVTINGG
Sbjct: 128 NFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTINGG 187

Query: 181 SASYACRFTETHRLVQERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTG 240
           SASYACRFT+T RLVQER LGRPVFPKAIGELHGHSGI RLLLFY RG+FGLVDHK GTG
Sbjct: 188 SASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTG 247

Query: 241 VANAGLVFFNDRLLAMSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPV 300
           VANAGLV+FNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDFN QL  +STMIAHPKVDP+
Sbjct: 248 VANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQL--HSTMIAHPKVDPI 307

Query: 301 SRELYALSYDVVHKPYLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVV 360
           S+ELYALSYDVV KPYLKYFRFSPDG KS DVEIPL+TPTMMHDFAITENFVVIPDQQVV
Sbjct: 308 SKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQVV 367

Query: 361 FKLGEMVKGGSPVIYDKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEE 420
           FKL EMVKGGSPVIYDK KKSRFGILPKNA+D+  +IWVESP+TFCFHLWNAWEEP TEE
Sbjct: 368 FKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEE 427

Query: 421 VVVIGSCMTPADSIFNECDENLKSVLSEIRLNLRTGKSTRRAIMAEE-EQVNLEAGMVNR 480
           VVVIGSCMTP DSIFNEC+ENLKSVLSEIRLNLRTGKSTRRAI+ EE EQ+NLEAGMVNR
Sbjct: 428 VVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMVNR 487

Query: 481 NRLGRRTRFAYLAIAEPWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNSAAE 540
           NRLGR+TRFAYLAIAEPWPKVSGFAKVDL TG+VKKHIYG RK+GGEPFFLP++ NS  E
Sbjct: 488 NRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSKEE 547

Query: 541 DDGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           D+GYILAFVHDERTWKSE+QIVNAMDLKVEAT+KLPSRVPYGFHGTFV SN LQNQA
Sbjct: 548 DEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 600

BLAST of MS011023 vs. NCBI nr
Match: XP_022926307.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 975.3 bits (2520), Expect = 2.3e-280
Identity = 481/585 (82.22%), Postives = 520/585 (88.89%), Query Frame = 0

Query: 2   SSSSSYSSSIC--NWKRQRPNNHKRSIIIQSALHSPSVLHFPNQNYHNKLSSLREDQDKP 61
           SSSSS    +C  N K ++ +N +R+ ++Q  LHSPSVLHFP+Q   NK   ++E+    
Sbjct: 5   SSSSSLFKPLCFDNIKIRQQSN-RRNNVVQCVLHSPSVLHFPDQTTSNKKLIVKEENKAS 64

Query: 62  IKDPNWNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLT 121
           I    WNF QK A+MA D+VESAL+S ELQHPLPKTADPRVQISGNFAPVPE P+KHNL 
Sbjct: 65  IS--GWNFLQKTASMALDMVESALLSFELQHPLPKTADPRVQISGNFAPVPEQPIKHNLP 124

Query: 122 VAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLV 181
           V GTIPDCINGVY+RNGANPLFEPTAGHHLFDGDGMVHAV+IN GSASYACRFTET RLV
Sbjct: 125 VTGTIPDCINGVYIRNGANPLFEPTAGHHLFDGDGMVHAVSINDGSASYACRFTETQRLV 184

Query: 182 QERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLA 241
           QER+LGR +FPKAIGELHGHSGI RLLLFY RGLFGLVDH+NGTGVANAGLVFFNDRLLA
Sbjct: 185 QERKLGRSIFPKAIGELHGHSGIVRLLLFYARGLFGLVDHRNGTGVANAGLVFFNDRLLA 244

Query: 242 MSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKP 301
           MSEDDLPYHVRITPSGDL+TVGRYDFN QL   STMIAHPKVDPVSRE+YALSYDVVHKP
Sbjct: 245 MSEDDLPYHVRITPSGDLETVGRYDFNTQL--QSTMIAHPKVDPVSREMYALSYDVVHKP 304

Query: 302 YLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIY 361
           YLKYFRFSPDG KS DVEIPLQTPTMMHDFAITENFV+IPDQQVVFKL EMVKGGSPVIY
Sbjct: 305 YLKYFRFSPDGIKSNDVEIPLQTPTMMHDFAITENFVIIPDQQVVFKLQEMVKGGSPVIY 364

Query: 362 DKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIF 421
           DK+KKSRFGILPKNA+D+  +IWVESPETFCFHLWNAWEE  TEEVVVIGSCMTP DSIF
Sbjct: 365 DKNKKSRFGILPKNATDSKDLIWVESPETFCFHLWNAWEEAETEEVVVIGSCMTPPDSIF 424

Query: 422 NECDENLKSVLSEIRLNLRTGKSTRRAIMAEEE-QVNLEAGMVNRNRLGRRTRFAYLAIA 481
           NECDENLKSVLSEIRLNLRTGKSTRR I+ EE+ QVNLEAGMVNRNRLGR+TR+AYLAIA
Sbjct: 425 NECDENLKSVLSEIRLNLRTGKSTRRPIITEEDHQVNLEAGMVNRNRLGRKTRYAYLAIA 484

Query: 482 EPWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNS---AAEDDGYILAFVHDE 541
           EPWPKVSGFAKVDL TG+VKKHIYG RK+GGEPFFLP++ NS     EDDG+IL FVHDE
Sbjct: 485 EPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEVNSEKEEEEDDGHILVFVHDE 544

Query: 542 RTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           RTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVD+  L+NQA
Sbjct: 545 RTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDATKLRNQA 584

BLAST of MS011023 vs. NCBI nr
Match: KAG7026460.1 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 973.0 bits (2514), Expect = 1.1e-279
Identity = 480/585 (82.05%), Postives = 518/585 (88.55%), Query Frame = 0

Query: 2   SSSSSYSSSIC--NWKRQRPNNHKRSIIIQSALHSPSVLHFPNQNYHNKLSSLREDQDKP 61
           SSSSS    +C  N K ++ +N +R+ ++Q  LHSPSVLHFP+Q   N    ++E+    
Sbjct: 4   SSSSSLFKPLCFDNIKIRQQSN-RRNNVVQCVLHSPSVLHFPDQTTSNNKLIVKEENKAS 63

Query: 62  IKDPNWNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLT 121
           I    WNF QK A+MA D+VES L+S ELQHPLPKTADPRVQISGNFAPVPE P+KHNL 
Sbjct: 64  IS--GWNFLQKTASMALDMVESVLLSFELQHPLPKTADPRVQISGNFAPVPEQPIKHNLP 123

Query: 122 VAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLV 181
           V GTIPDCINGVY+RNGANPLFEPTAGHHLFDGDGMVHAV+IN GSASYACRFTET RLV
Sbjct: 124 VTGTIPDCINGVYIRNGANPLFEPTAGHHLFDGDGMVHAVSINDGSASYACRFTETQRLV 183

Query: 182 QERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLA 241
           QER+LGR +FPKAIGELHGHSGI RLLLFY RGLFGLVDH+NGTGVANAGLVFFNDRLLA
Sbjct: 184 QERKLGRSIFPKAIGELHGHSGIVRLLLFYARGLFGLVDHRNGTGVANAGLVFFNDRLLA 243

Query: 242 MSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKP 301
           MSEDDLPYHVRITPSGDL+TVGRYDFN QL   STMIAHPKVDPVSRE+YALSYDVVHKP
Sbjct: 244 MSEDDLPYHVRITPSGDLETVGRYDFNTQL--QSTMIAHPKVDPVSREMYALSYDVVHKP 303

Query: 302 YLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIY 361
           YLKYFRFSPDG KS DVEIPLQTPTMMHDFAITENFV+IPDQQVVFKL EMVKGGSPVIY
Sbjct: 304 YLKYFRFSPDGIKSNDVEIPLQTPTMMHDFAITENFVIIPDQQVVFKLQEMVKGGSPVIY 363

Query: 362 DKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIF 421
           DK+KKSRFGILPKNA+D+  +IWVESPETFCFHLWNAWEE  TEEVVVIGSCMTP DSIF
Sbjct: 364 DKNKKSRFGILPKNATDSKDLIWVESPETFCFHLWNAWEEAETEEVVVIGSCMTPPDSIF 423

Query: 422 NECDENLKSVLSEIRLNLRTGKSTRRAIMAEEE-QVNLEAGMVNRNRLGRRTRFAYLAIA 481
           NECDENLKSVLSEIRLNLRTGKSTRR I+ EEE QVNLEAGMVNRNRLGR+TR+AYLAIA
Sbjct: 424 NECDENLKSVLSEIRLNLRTGKSTRRPIITEEEQQVNLEAGMVNRNRLGRKTRYAYLAIA 483

Query: 482 EPWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNS---AAEDDGYILAFVHDE 541
           EPWPKVSGFAKVDL TG+VKKHIYG RK+GGEPFFLP++ NS     EDDG+IL FVHDE
Sbjct: 484 EPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEVNSEKEEEEDDGHILVFVHDE 543

Query: 542 RTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           RTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVD+  L+NQA
Sbjct: 544 RTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDATKLRNQA 583

BLAST of MS011023 vs. NCBI nr
Match: KAG6594462.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 972.6 bits (2513), Expect = 1.5e-279
Identity = 479/585 (81.88%), Postives = 518/585 (88.55%), Query Frame = 0

Query: 2   SSSSSYSSSIC--NWKRQRPNNHKRSIIIQSALHSPSVLHFPNQNYHNKLSSLREDQDKP 61
           SSSSS    +C  N K ++ +N +R+ ++Q  LHSPSVLHFP+Q   N    ++E+    
Sbjct: 4   SSSSSLFKPLCFDNIKIRQQSN-RRNNVVQCVLHSPSVLHFPDQTTSNNKLIVKEENKAS 63

Query: 62  IKDPNWNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLT 121
           I    WNF QK A+MA D+VES L+S ELQHPLPKTADPRVQISGNFAPVPE P+KHNL 
Sbjct: 64  IS--GWNFLQKTASMALDMVESVLLSFELQHPLPKTADPRVQISGNFAPVPEQPIKHNLP 123

Query: 122 VAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLV 181
           V GTIPDCINGVY+RNGANPLFEPTAGHHLFDGDGMVHAV+IN GSASYACRFTET RLV
Sbjct: 124 VTGTIPDCINGVYIRNGANPLFEPTAGHHLFDGDGMVHAVSINDGSASYACRFTETQRLV 183

Query: 182 QERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLA 241
           QER+LGR +FPKAIGELHGHSGI RLLLFY RGLFGLVDH+NGTGVANAGLVFFNDRLLA
Sbjct: 184 QERKLGRSIFPKAIGELHGHSGIVRLLLFYARGLFGLVDHRNGTGVANAGLVFFNDRLLA 243

Query: 242 MSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKP 301
           MSEDDLPYHVRITPSGDL+TVGRYDFN QL   STMIAHPKVDP+SRE+YALSYDVVHKP
Sbjct: 244 MSEDDLPYHVRITPSGDLETVGRYDFNTQL--QSTMIAHPKVDPISREMYALSYDVVHKP 303

Query: 302 YLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIY 361
           YLKYFRFSPDG KS DVEIPLQTPTMMHDFAITENFV+IPDQQVVFKL EMVKGGSPVIY
Sbjct: 304 YLKYFRFSPDGIKSNDVEIPLQTPTMMHDFAITENFVIIPDQQVVFKLQEMVKGGSPVIY 363

Query: 362 DKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIF 421
           DK+KKSRFGILPKNA+D+  +IWVESPETFCFHLWNAWEE  TEEVVVIGSCMTP DSIF
Sbjct: 364 DKNKKSRFGILPKNATDSKDLIWVESPETFCFHLWNAWEEAETEEVVVIGSCMTPPDSIF 423

Query: 422 NECDENLKSVLSEIRLNLRTGKSTRRAIMAEEE-QVNLEAGMVNRNRLGRRTRFAYLAIA 481
           NECDENLKSVLSEIRLNLRTGKSTRR I+ EEE QVNLEAGMVNRNRLGR+TR+AYLAIA
Sbjct: 424 NECDENLKSVLSEIRLNLRTGKSTRRPIITEEEQQVNLEAGMVNRNRLGRKTRYAYLAIA 483

Query: 482 EPWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNS---AAEDDGYILAFVHDE 541
           EPWPKVSGFAKVDL TG+VKKHIYG RK+GGEPFFLP++ NS     EDDG+IL FVHDE
Sbjct: 484 EPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEVNSEKEEEEDDGHILVFVHDE 543

Query: 542 RTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           RTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVD+  L+NQA
Sbjct: 544 RTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDATKLRNQA 583

BLAST of MS011023 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 5.9e-263
Identity = 436/581 (75.04%), Postives = 498/581 (85.71%), Query Frame = 0

Query: 8   SSSICNWKRQRPNNHKRSIIIQSALHSPSVL---HFPNQ-NYHNKLSSLREDQDKPIKDP 67
           +S+I N++     +   S  +   L S S+    H   +   H+ L +L +  + P   P
Sbjct: 2   TSTIANYRVSHSFSPSTSYSLDFTLPSKSISMKNHTTTKTKIHSALLTLPKQNNTPKNQP 61

Query: 68  -----NWNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNL 127
                +WNFFQKAAA A D+VESAL+S+ELQ+PLPKTADPRVQI+GNFAPVPE  V+HNL
Sbjct: 62  QFQTSHWNFFQKAAAKALDIVESALVSRELQNPLPKTADPRVQIAGNFAPVPEQSVRHNL 121

Query: 128 TVAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRL 187
            V GTIPDCINGVY+RNGANPLFEP AGHHLFDGDGMVHAVT+  GS SY+CRFTET RL
Sbjct: 122 PVTGTIPDCINGVYVRNGANPLFEPVAGHHLFDGDGMVHAVTVENGSVSYSCRFTETERL 181

Query: 188 VQERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLL 247
           VQERELG PVFPKAIGELHGHSGI RLLLFY RG+FGLVDH +GTGVANAGLVFFN+RLL
Sbjct: 182 VQERELGHPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHSHGTGVANAGLVFFNNRLL 241

Query: 248 AMSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHK 307
           AMSEDD+PYHV++ PSGDL+TVGRY+F++QL   STMIAHPK+DPVS EL+ALSYDVV K
Sbjct: 242 AMSEDDVPYHVQVLPSGDLQTVGRYNFDDQL--KSTMIAHPKIDPVSGELFALSYDVVQK 301

Query: 308 PYLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVI 367
           PYLK F+FSPDG KSPDVEIPL  PTMMHDFAITEN+VVIPDQQVVFKL EM+KGGSPVI
Sbjct: 302 PYLKSFKFSPDGEKSPDVEIPLDVPTMMHDFAITENYVVIPDQQVVFKLQEMIKGGSPVI 361

Query: 368 YDKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSI 427
           YDK+KKSRFGILPKNA ++  IIWVES ETFCFHLWNAWEEP T+EV+VIGSCMTP DSI
Sbjct: 362 YDKNKKSRFGILPKNAENSENIIWVESAETFCFHLWNAWEEPETDEVIVIGSCMTPPDSI 421

Query: 428 FNECDENLKSVLSEIRLNLRTGKSTRRAIMAEEEQVNLEAGMVNRNRLGRRTRFAYLAIA 487
           FNEC+ENLKSVLSEIRLNL+TG+STRR +++  +QVNLEAGMVNRN+LGR+T+FAYLAIA
Sbjct: 422 FNECNENLKSVLSEIRLNLKTGESTRRQLLSPSDQVNLEAGMVNRNKLGRKTQFAYLAIA 481

Query: 488 EPWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNSAAEDDGYILAFVHDERTW 547
           EPWPKVSGFAKVDL+TG++KKHIYGD++YGGEP FLPR+ NS  EDDGYILAF HDE+TW
Sbjct: 482 EPWPKVSGFAKVDLSTGEIKKHIYGDKRYGGEPLFLPRNVNSEKEDDGYILAFCHDEKTW 541

Query: 548 KSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQ 580
           KSELQIVNAM L++EATVKLPSRVPYGFHGTF+ S DLQNQ
Sbjct: 542 KSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQNQ 580

BLAST of MS011023 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 879.8 bits (2272), Expect = 1.7e-254
Identity = 428/582 (73.54%), Postives = 488/582 (83.85%), Query Frame = 0

Query: 8   SSSICNWKRQRPNNHKRSIIIQSALHSPSVLHFPNQ--NY----HNKLSSLREDQDKPIK 67
           S+SI   K Q   ++++S+ I S+L +P +LHFP Q  NY    +N +S  +++ +    
Sbjct: 29  SNSISLLKNQ---HNRQSLNINSSLQAPPILHFPKQSSNYQTPKNNTISHPKQENNNSSS 88

Query: 68  D--PNWNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLT 127
                WN  QKAAAMA D VESAL   EL+HPLPKTADPRVQISGNFAPVPE+PV  +L 
Sbjct: 89  SSTSKWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPVPENPVCQSLP 148

Query: 128 VAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLV 187
           V G IP C+ GVY+RNGANPLFEPTAGHH FDGDGMVHAV    GSASYACRFTET RLV
Sbjct: 149 VTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLV 208

Query: 188 QERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLA 247
           QE+ LGRPVFPKAIGELHGHSGI RL+LFY RGLFGLVDH  GTGVANAGLV+FN+RLLA
Sbjct: 209 QEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANAGLVYFNNRLLA 268

Query: 248 MSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKP 307
           MSEDDLPYHV++TP+GDLKT GR+DF+ QL   STMIAHPK+DPVS EL+ALSYDV+ KP
Sbjct: 269 MSEDDLPYHVKVTPTGDLKTEGRFDFDGQL--KSTMIAHPKLDPVSGELFALSYDVIQKP 328

Query: 308 YLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIY 367
           YLKYFRFS +G KS DVEIP++ PTMMHDFAITENFVVIPDQQVVFK+ EM++GGSPV+Y
Sbjct: 329 YLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITENFVVIPDQQVVFKMSEMIRGGSPVVY 388

Query: 368 DKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIF 427
           DK+K SRFGIL K A D S + WVE P+ FCFHLWNAWEE  T+E+VVIGSCMTP DSIF
Sbjct: 389 DKNKVSRFGILDKYAKDGSDLKWVEVPDCFCFHLWNAWEEAETDEIVVIGSCMTPPDSIF 448

Query: 428 NECDENLKSVLSEIRLNLRTGKSTRRAIMAE-EEQVNLEAGMVNRNRLGRRTRFAYLAIA 487
           NECDE LKSVLSEIRLNL+TGKSTR++I+   +EQVNLEAGMVNRN+LGR+T +AYLAIA
Sbjct: 449 NECDEGLKSVLSEIRLNLKTGKSTRKSIIENPDEQVNLEAGMVNRNKLGRKTEYAYLAIA 508

Query: 488 EPWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNSAAEDDGYILAFVHDERTW 547
           EPWPKVSGFAKV+L TG+V+K IYGD KYGGEP FLPRDPNS  EDDGYILAFVHDE+ W
Sbjct: 509 EPWPKVSGFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRDPNSKEEDDGYILAFVHDEKEW 568

Query: 548 KSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           KSELQIVNAM LK+EATVKLPSRVPYGFHGTF+++NDL NQA
Sbjct: 569 KSELQIVNAMSLKLEATVKLPSRVPYGFHGTFINANDLANQA 605

BLAST of MS011023 vs. ExPASy Swiss-Prot
Match: Q9C6Z1 (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED5 PE=1 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 7.9e-244
Identity = 413/581 (71.08%), Postives = 477/581 (82.10%), Query Frame = 0

Query: 1   PSSSSSYSSSICNWKRQRPNNHKRSIIIQSALHSPSVLHFPNQNYHNKLSSLREDQDKPI 60
           PS SSS   S  N K +R     R +   S   +P++L+FPN    N +         P 
Sbjct: 26  PSPSSSV--SFTNTKPRR-----RKLSANSVSDTPNLLNFPNYPSPNPII--------PE 85

Query: 61  KDPN-WNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLT 120
           KD + WN  Q+AA+ A D  E+AL+ +E   PLPKT DPR QISGN+APVPE  VK +L+
Sbjct: 86  KDTSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLS 145

Query: 121 VAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLV 180
           V G IPDCI+GVYLRNGANPLFEP +GHHLFDGDGMVHAV I  G ASY+CRFTET RLV
Sbjct: 146 VDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLV 205

Query: 181 QERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLA 240
           QE++LG P+FPKAIGELHGHSGI RL+LFY RGLFGL++HKNGTGVANAGLV+F+DRLLA
Sbjct: 206 QEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLA 265

Query: 241 MSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKP 300
           MSEDDLPY VR+T +GDL+T+GR+DF+ QL  +S MIAHPK+DPV++EL+ALSYDVV KP
Sbjct: 266 MSEDDLPYQVRVTDNGDLETIGRFDFDGQL--SSAMIAHPKIDPVTKELFALSYDVVKKP 325

Query: 301 YLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIY 360
           YLKYF+FSP+G KSPDVEIPL +PTMMHDFAITENFVVIPDQQVVFKL +M  G SPV Y
Sbjct: 326 YLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKY 385

Query: 361 DKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIF 420
           D +K SRFGILP+NA DAS ++WVESPETFCFHLWNAWE P T+EVVVIGSCMTPADSIF
Sbjct: 386 DGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIF 445

Query: 421 NECDENLKSVLSEIRLNLRTGKSTRRAIMAEEEQVNLEAGMVNRNRLGRRTRFAYLAIAE 480
           NECDE L SVLSEIRLNL+TGKSTRR I+    Q+NLEAGMVNRN LGR+TR+AYLAIAE
Sbjct: 446 NECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAE 505

Query: 481 PWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNSAAEDDGYILAFVHDERTWK 540
           PWPKVSGFAKVDL+TG+VK H YG +KYGGEPFFLPR   S  EDDGYI++FVHDE +W+
Sbjct: 506 PWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWE 565

Query: 541 SELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           SEL IVNA+ L++EATVKLPSRVPYGFHGTFV+S D+ NQA
Sbjct: 566 SELHIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQA 589

BLAST of MS011023 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 2.8e-241
Identity = 407/595 (68.40%), Postives = 475/595 (79.83%), Query Frame = 0

Query: 2   SSSSSYSSSICNWKRQRPNNHKRSIIIQSALHSP----------SVLHFPNQNYHNKLSS 61
           +S+SS  +++  +K++  +N          LH P          +    P +      ++
Sbjct: 26  ASTSSSPTTLLPFKKRSSSNTNTITCSLQTLHYPKQYQPTSTSTTTTPTPIKPTTTTTTT 85

Query: 62  LREDQDKPIKD------PNWNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGN 121
               + KP+ D        WNF QKAAA   D+VE+AL+S E +HPLPKTADP+VQI+GN
Sbjct: 86  TPHRETKPLSDTKQPFPQKWNFLQKAAATGLDMVETALVSHESKHPLPKTADPKVQIAGN 145

Query: 122 FAPVPEHPVKHNLTVAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGS 181
           FAPVPEH     L V G IP CI+GVY+RNGANPL+EP AGHH FDGDGMVHAV    G+
Sbjct: 146 FAPVPEHAADQALPVVGKIPKCIDGVYVRNGANPLYEPVAGHHFFDGDGMVHAVKFTNGA 205

Query: 182 ASYACRFTETHRLVQERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGV 241
           ASYACRFTET RL QE+ LGRPVFPKAIGELHGHSGI RLLLFY R LF LVD  +G GV
Sbjct: 206 ASYACRFTETQRLAQEKSLGRPVFPKAIGELHGHSGIARLLLFYARSLFQLVDGSHGMGV 265

Query: 242 ANAGLVFFNDRLLAMSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVS 301
           ANAGLV+FN+ LLAMSEDDLPYHVRIT +GDL TVGRYDFN QL  NSTMIAHPK+DPV+
Sbjct: 266 ANAGLVYFNNHLLAMSEDDLPYHVRITSNGDLTTVGRYDFNGQL--NSTMIAHPKLDPVN 325

Query: 302 RELYALSYDVVHKPYLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVF 361
            +L+ALSYDVV KPYLKYFRFS DG KSPDVEIPL+ PTMMHDFAITENFVV+PDQQVVF
Sbjct: 326 GDLHALSYDVVQKPYLKYFRFSADGVKSPDVEIPLKEPTMMHDFAITENFVVVPDQQVVF 385

Query: 362 KLGEMVKGGSPVIYDKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEV 421
           KL EM+ GGSPV+YDK+K SRFGIL KNA DA+ + W+++PE FCFHLWNAWEEP T+E+
Sbjct: 386 KLTEMITGGSPVVYDKNKTSRFGILDKNAKDANAMRWIDAPECFCFHLWNAWEEPETDEI 445

Query: 422 VVIGSCMTPADSIFNECDENLKSVLSEIRLNLRTGKSTRRAIMAEEEQVNLEAGMVNRNR 481
           VVIGSCMTPADSIFNECDE+LKSVLSEIRLNLRTGKSTRR I+++ EQVNLEAGMVNRN+
Sbjct: 446 VVIGSCMTPADSIFNECDESLKSVLSEIRLNLRTGKSTRRPIISDAEQVNLEAGMVNRNK 505

Query: 482 LGRRTRFAYLAIAEPWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNSAAEDD 541
           LGR+T+FAYLA+AEPWPKVSGFAKVDL +G+V+K++YG+ K+GGEP FL   PN   E D
Sbjct: 506 LGRKTQFAYLALAEPWPKVSGFAKVDLFSGEVQKYMYGEEKFGGEPLFL---PNGEEEGD 565

Query: 542 GYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           GYILAFVHDE+ WKSELQIVNA +LK+EA++KLPSRVPYGFHGTF+ S DL+ QA
Sbjct: 566 GYILAFVHDEKEWKSELQIVNAQNLKLEASIKLPSRVPYGFHGTFIHSKDLRKQA 615

BLAST of MS011023 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 3.1e-240
Identity = 408/579 (70.47%), Postives = 469/579 (81.00%), Query Frame = 0

Query: 2   SSSSSYSSSICNWKRQRPNNHKRSIIIQSALHSPSVLHFPNQNYHNKLSSLREDQDKPIK 61
           SSS S   S C+      +   R + + SALH+P  LHFP Q+  N  + + + + K   
Sbjct: 25  SSSQSSDLSYCS-SLPMASRVTRKLNVSSALHTPPALHFPKQS-SNSPAIVVKPKAKESN 84

Query: 62  DPNWNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLTVA 121
               N FQ+AAA A D  E  L+S E  HPLPKTADP VQI+GNFAPV E PV+ NL V 
Sbjct: 85  TKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVV 144

Query: 122 GTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLVQE 181
           G +PD I GVY+RNGANPL EP  GHH FDGDGMVHAV    GSASYACRFT+T+R VQE
Sbjct: 145 GKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQE 204

Query: 182 RELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLAMS 241
           R+LGRPVFPKAIGELHGH+GI RL+LFY R   G+VD  +GTGVANAGLV+FN RLLAMS
Sbjct: 205 RQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMS 264

Query: 242 EDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKPYL 301
           EDDLPY V+ITP+GDLKTVGR+DF+ QL   STMIAHPKVDP S EL+ALSYDVV KPYL
Sbjct: 265 EDDLPYQVQITPNGDLKTVGRFDFDGQL--ESTMIAHPKVDPESGELFALSYDVVSKPYL 324

Query: 302 KYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIYDK 361
           KYFRFSPDGTKSPDVEI L  PTMMHDFAITENFVV+PDQQVVFKL EM++GGSPV+YDK
Sbjct: 325 KYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYDK 384

Query: 362 DKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIFNE 421
           +K +RFGIL K A D+S I W+++P+ FCFHLWNAWEEP T+EVVVIGSCMTP DSIFNE
Sbjct: 385 NKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVVIGSCMTPPDSIFNE 444

Query: 422 CDENLKSVLSEIRLNLRTGKSTRRAIMA-EEEQVNLEAGMVNRNRLGRRTRFAYLAIAEP 481
            DENLKSVLSEIRLNL+TG+STRR I++ E++QVNLEAGMVNRN LGR+T+FAYLA+AEP
Sbjct: 445 SDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEP 504

Query: 482 WPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNSAAEDDGYILAFVHDERTWKS 541
           WPKVSGFAKVDL TG+VKKH+YGD +YGGEP FLP +     ED+GYIL FVHDE+TWKS
Sbjct: 505 WPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGE--GGEEDEGYILCFVHDEKTWKS 564

Query: 542 ELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQ 580
           ELQIVNA+ L+VEATVKLPSRVPYGFHGTF+ ++DL  Q
Sbjct: 565 ELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQ 597

BLAST of MS011023 vs. ExPASy TrEMBL
Match: A0A1L5JHV5 (9-cis-epoxycarotenoid dioxygenase NCED2 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 6.2e-284
Identity = 487/586 (83.11%), Postives = 521/586 (88.91%), Query Frame = 0

Query: 3   SSSSYSSSICNWK-RQRPNNHKRSIIIQSALHSPSVLHFPNQ-NYHNKLSSLREDQDKPI 62
           S+S+ S    N K  Q+ NN    +++  ALHSPSVLHFPNQ NYH KL    +      
Sbjct: 19  SNSTKSIRFKNVKISQQSNNRNAVLVVHCALHSPSVLHFPNQTNYHKKLVLKEDTNTNKP 78

Query: 63  KDP--NWNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNL 122
           K P  NWNF QK A+MAFD+VESAL+S ELQHPLPKTADPRVQISGNFAPVPE PVKHNL
Sbjct: 79  KPPNSNWNFLQKTASMAFDIVESALLSHELQHPLPKTADPRVQISGNFAPVPEQPVKHNL 138

Query: 123 TVAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRL 182
            + GTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAV+ING +ASYACRFTET RL
Sbjct: 139 PITGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVSINGATASYACRFTETQRL 198

Query: 183 VQERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLL 242
           VQER LGR VFPKAIGELHGHSGI RLLLFYGRGLFGLVDHKNGTGVANAGLV+FNDRLL
Sbjct: 199 VQERRLGRSVFPKAIGELHGHSGIARLLLFYGRGLFGLVDHKNGTGVANAGLVYFNDRLL 258

Query: 243 AMSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHK 302
           AMSEDDLPYHVRIT SGDL+TVGRYDF  QL   STMIAHPKVDPVSRELYALSYDVV K
Sbjct: 259 AMSEDDLPYHVRITASGDLETVGRYDFKMQL--KSTMIAHPKVDPVSRELYALSYDVVRK 318

Query: 303 PYLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVI 362
           PYLKYFRFSPDGTKS D+EIPL TPTMMHDFAITENFVVIPDQQVVFKL EMVKGGSPVI
Sbjct: 319 PYLKYFRFSPDGTKSKDLEIPLHTPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVI 378

Query: 363 YDKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSI 422
           YDK+KKSRFGILPKNASD++ +IWVESP+TFCFHLWNAWEEP T+EVVVIGSCMTP DSI
Sbjct: 379 YDKNKKSRFGILPKNASDSNDLIWVESPDTFCFHLWNAWEEPETDEVVVIGSCMTPPDSI 438

Query: 423 FNECDENLKSVLSEIRLNLRTGKSTRRAIMA-EEEQVNLEAGMVNRNRLGRRTRFAYLAI 482
           FNECDENLKSVLSEIRLNLRTGKSTRR I++ EE+QVNLEAGMVNRNRLGR+TR+AYLAI
Sbjct: 439 FNECDENLKSVLSEIRLNLRTGKSTRRPIISDEEQQVNLEAGMVNRNRLGRKTRYAYLAI 498

Query: 483 AEPWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNS---AAEDDGYILAFVHD 542
           AEPWPKVSGFAKVDL TG+VKKHIYG RK+GGEPFFLP++  S     ED+GYILAFVHD
Sbjct: 499 AEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGKSEKEEEEDEGYILAFVHD 558

Query: 543 ERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           ERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVD+N+LQNQA
Sbjct: 559 ERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDANNLQNQA 602

BLAST of MS011023 vs. ExPASy TrEMBL
Match: A0A6J1EE62 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433499 PE=3 SV=1)

HSP 1 Score: 975.3 bits (2520), Expect = 1.1e-280
Identity = 481/585 (82.22%), Postives = 520/585 (88.89%), Query Frame = 0

Query: 2   SSSSSYSSSIC--NWKRQRPNNHKRSIIIQSALHSPSVLHFPNQNYHNKLSSLREDQDKP 61
           SSSSS    +C  N K ++ +N +R+ ++Q  LHSPSVLHFP+Q   NK   ++E+    
Sbjct: 5   SSSSSLFKPLCFDNIKIRQQSN-RRNNVVQCVLHSPSVLHFPDQTTSNKKLIVKEENKAS 64

Query: 62  IKDPNWNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLT 121
           I    WNF QK A+MA D+VESAL+S ELQHPLPKTADPRVQISGNFAPVPE P+KHNL 
Sbjct: 65  IS--GWNFLQKTASMALDMVESALLSFELQHPLPKTADPRVQISGNFAPVPEQPIKHNLP 124

Query: 122 VAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLV 181
           V GTIPDCINGVY+RNGANPLFEPTAGHHLFDGDGMVHAV+IN GSASYACRFTET RLV
Sbjct: 125 VTGTIPDCINGVYIRNGANPLFEPTAGHHLFDGDGMVHAVSINDGSASYACRFTETQRLV 184

Query: 182 QERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLA 241
           QER+LGR +FPKAIGELHGHSGI RLLLFY RGLFGLVDH+NGTGVANAGLVFFNDRLLA
Sbjct: 185 QERKLGRSIFPKAIGELHGHSGIVRLLLFYARGLFGLVDHRNGTGVANAGLVFFNDRLLA 244

Query: 242 MSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKP 301
           MSEDDLPYHVRITPSGDL+TVGRYDFN QL   STMIAHPKVDPVSRE+YALSYDVVHKP
Sbjct: 245 MSEDDLPYHVRITPSGDLETVGRYDFNTQL--QSTMIAHPKVDPVSREMYALSYDVVHKP 304

Query: 302 YLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIY 361
           YLKYFRFSPDG KS DVEIPLQTPTMMHDFAITENFV+IPDQQVVFKL EMVKGGSPVIY
Sbjct: 305 YLKYFRFSPDGIKSNDVEIPLQTPTMMHDFAITENFVIIPDQQVVFKLQEMVKGGSPVIY 364

Query: 362 DKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIF 421
           DK+KKSRFGILPKNA+D+  +IWVESPETFCFHLWNAWEE  TEEVVVIGSCMTP DSIF
Sbjct: 365 DKNKKSRFGILPKNATDSKDLIWVESPETFCFHLWNAWEEAETEEVVVIGSCMTPPDSIF 424

Query: 422 NECDENLKSVLSEIRLNLRTGKSTRRAIMAEEE-QVNLEAGMVNRNRLGRRTRFAYLAIA 481
           NECDENLKSVLSEIRLNLRTGKSTRR I+ EE+ QVNLEAGMVNRNRLGR+TR+AYLAIA
Sbjct: 425 NECDENLKSVLSEIRLNLRTGKSTRRPIITEEDHQVNLEAGMVNRNRLGRKTRYAYLAIA 484

Query: 482 EPWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNS---AAEDDGYILAFVHDE 541
           EPWPKVSGFAKVDL TG+VKKHIYG RK+GGEPFFLP++ NS     EDDG+IL FVHDE
Sbjct: 485 EPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEVNSEKEEEEDDGHILVFVHDE 544

Query: 542 RTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           RTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVD+  L+NQA
Sbjct: 545 RTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDATKLRNQA 584

BLAST of MS011023 vs. ExPASy TrEMBL
Match: A0A6J1EH85 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433338 PE=3 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.6e-279
Identity = 475/570 (83.33%), Postives = 514/570 (90.18%), Query Frame = 0

Query: 17  QRPNNHKRSIIIQSALHSPSVLHFPNQ--NYHNKLSSLREDQDKPIKDP--NWNFFQKAA 76
           ++P+NH  + +I SALHSPSVLHFPN   N+H+ L          IK P  NWNF QK+A
Sbjct: 33  RQPSNH--NAVIHSALHSPSVLHFPNHSPNFHDNL----------IKSPNSNWNFLQKSA 92

Query: 77  AMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLTVAGTIPDCINGVY 136
           ++A D+ ESAL+S EL+HPLPKTADPRVQISGNFAPVPE PVKHNL V GTIPDCINGVY
Sbjct: 93  SIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVY 152

Query: 137 LRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLVQERELGRPVFPKA 196
           LRNGANPLFEPTAGHHLFDGDGMVHAVTIN GSASYACRFT+T RL QE++LGRP+FPKA
Sbjct: 153 LRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKA 212

Query: 197 IGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLAMSEDDLPYHVRIT 256
           IGELHGHSGI RLLLFY RG+FGLVDHK GTGVANAGLV+FNDRLLAMSEDDLPYHVRIT
Sbjct: 213 IGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRIT 272

Query: 257 PSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKPYLKYFRFSPDGTK 316
           PSGDL+TVGRYDFN QL  +STMIAHPKVDPVSRELYALSYDVV KPYLKYFRFSPDG K
Sbjct: 273 PSGDLETVGRYDFNTQL--DSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIK 332

Query: 317 SPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIYDKDKKSRFGILPK 376
           S DVEIPLQTPTMMHDFAITENFVVIPDQQVVFKL EM+KGGSPVIYDK+KKSRFGILPK
Sbjct: 333 SKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPK 392

Query: 377 NASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIFNECDENLKSVLSE 436
           NA+++  +IWVESP+TFCFHLWNAWEEP TEEVVVIGSCMTP DSIFNECDENLKSVLSE
Sbjct: 393 NATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSE 452

Query: 437 IRLNLRTGKSTRRAIMAEE-EQVNLEAGMVNRNRLGRRTRFAYLAIAEPWPKVSGFAKVD 496
           IRLNLRTGKSTRR I+++E EQVNLEAGMVNRNRLGR+TR+AYLAIAEPWPKVSGFAKVD
Sbjct: 453 IRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVD 512

Query: 497 LATGQVKKHIYGDRKYGGEPFFLPRDPNSA-AEDDGYILAFVHDERTWKSELQIVNAMDL 556
           L TG+VKKHIYG RK+GGEPFFL ++ NS   ED+GYILAFVHDERTWKSELQIVNAMDL
Sbjct: 513 LVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDL 572

Query: 557 KVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           KVEATVKLPSRVPYGFHGTFVD+  LQNQA
Sbjct: 573 KVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of MS011023 vs. ExPASy TrEMBL
Match: A0A6J1IVE0 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478741 PE=3 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 1.0e-278
Identity = 473/570 (82.98%), Postives = 514/570 (90.18%), Query Frame = 0

Query: 17  QRPNNHKRSIIIQSALHSPSVLHFPNQ--NYHNKLSSLREDQDKPIKDP--NWNFFQKAA 76
           ++P+NH  + +I SALHSPSVLHFPN   N+H+ L          IK P  NWNF QK+A
Sbjct: 33  RQPSNH--NAVIHSALHSPSVLHFPNHSPNFHDNL----------IKSPNSNWNFLQKSA 92

Query: 77  AMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLTVAGTIPDCINGVY 136
           ++A D+ ESAL+S EL+HPLPKTADPRVQISGNFAPVPE PVK+NL V GTIPDCINGVY
Sbjct: 93  SIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQPVKYNLPVTGTIPDCINGVY 152

Query: 137 LRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLVQERELGRPVFPKA 196
           LRNGANPLFEPTAGHHLFDGDGMVHAVTIN GSASYACRFT+T RL QE++LGRP+FPKA
Sbjct: 153 LRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKA 212

Query: 197 IGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLAMSEDDLPYHVRIT 256
           IGELHGHSGI RLLLFY RG+FGLVDHK GTGVANAGLV+FNDRLLAMSEDDLPYHVRIT
Sbjct: 213 IGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRIT 272

Query: 257 PSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKPYLKYFRFSPDGTK 316
           PSGDL+TVGRYDFN QL  +STMIAHPKVDPVSRELYALSYDVV KPYLKYFRFSPDG K
Sbjct: 273 PSGDLETVGRYDFNTQL--DSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIK 332

Query: 317 SPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIYDKDKKSRFGILPK 376
           S DVEIPLQTPTMMHDFAITENFVVIPDQQVVFKL EM+KGGSPVIYDK+KKSRFGILPK
Sbjct: 333 SKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPK 392

Query: 377 NASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIFNECDENLKSVLSE 436
           NA+++  +IWVESP+TFCFHLWNAWEEP TEEVVVIGSCMTP DSIFNECDENLKSVLSE
Sbjct: 393 NATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSE 452

Query: 437 IRLNLRTGKSTRRAIMAEE-EQVNLEAGMVNRNRLGRRTRFAYLAIAEPWPKVSGFAKVD 496
           IRLNLRTGKSTRR I+++E EQVNLEAGMVNRNRLGR+TR+AYLAIAEPWPKVSGFAKVD
Sbjct: 453 IRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVD 512

Query: 497 LATGQVKKHIYGDRKYGGEPFFLPRDPNSA-AEDDGYILAFVHDERTWKSELQIVNAMDL 556
           L TG+VKKHIYG RK+GGEPFFL ++ NS   ED+GYILAFVHDERTWKSELQIVNAMDL
Sbjct: 513 LVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDL 572

Query: 557 KVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           KVEATVKLPSRVPYGFHGTF+D+  LQNQA
Sbjct: 573 KVEATVKLPSRVPYGFHGTFIDAKHLQNQA 588

BLAST of MS011023 vs. ExPASy TrEMBL
Match: A0A2Z4GT57 (9-cis-epoxycarotenoid dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 1.3e-278
Identity = 474/570 (83.16%), Postives = 513/570 (90.00%), Query Frame = 0

Query: 17  QRPNNHKRSIIIQSALHSPSVLHFPNQ--NYHNKLSSLREDQDKPIKDP--NWNFFQKAA 76
           ++P+NH  + +I SALHSPSVLHFPN   N+H+ L          IK P  NWNF QK+A
Sbjct: 33  RQPSNH--NALIHSALHSPSVLHFPNHSPNFHDNL----------IKSPNSNWNFLQKSA 92

Query: 77  AMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLTVAGTIPDCINGVY 136
           ++A D+ ESAL+S EL+HPLPKTADPRVQISGNFAPVPE PVKHNL V GTIPDCINGVY
Sbjct: 93  SIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVY 152

Query: 137 LRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLVQERELGRPVFPKA 196
           LRNGANPLFEPTAGHHLFDGDGMVHAVTIN GSASYACRFT+T RL QE++LGRP+FPKA
Sbjct: 153 LRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKA 212

Query: 197 IGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLAMSEDDLPYHVRIT 256
           IGELHGHSGI RLLLFY RG+FGLVDHK GTGVANAGLV+FNDRLLAMSEDDLPYHVRIT
Sbjct: 213 IGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRIT 272

Query: 257 PSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKPYLKYFRFSPDGTK 316
           PSGDL+TVGRYDFN QL  +STMIAHPKVDPVSR LYALSYDVV KPYLKYFRFSPDG K
Sbjct: 273 PSGDLETVGRYDFNTQL--DSTMIAHPKVDPVSRGLYALSYDVVRKPYLKYFRFSPDGIK 332

Query: 317 SPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIYDKDKKSRFGILPK 376
           S DVEIPLQTPTMMHDFAITENFVVIPDQQVVFKL EM+KGGSPVIYDK+KKSRFGILPK
Sbjct: 333 SQDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPK 392

Query: 377 NASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIFNECDENLKSVLSE 436
           NA+++  +IWVESP+TFCFHLWNAWEEP TEEVVVIGSCMTP DSIFNECDENLKSVLSE
Sbjct: 393 NATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSE 452

Query: 437 IRLNLRTGKSTRRAIMAEE-EQVNLEAGMVNRNRLGRRTRFAYLAIAEPWPKVSGFAKVD 496
           IRLNLRTGKSTRR I+++E EQVNLEAGMVNRNRLGR+TR+AYLAIAEPWPKVSGFAKVD
Sbjct: 453 IRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVD 512

Query: 497 LATGQVKKHIYGDRKYGGEPFFLPRDPNSA-AEDDGYILAFVHDERTWKSELQIVNAMDL 556
           L TG+VKKHIYG RK+GGEPFFL ++ NS   ED+GYILAFVHDERTWKSELQIVNAMDL
Sbjct: 513 LVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDL 572

Query: 557 KVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           KVEATVKLPSRVPYGFHGTFVD+  LQNQA
Sbjct: 573 KVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of MS011023 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 844.3 bits (2180), Expect = 5.6e-245
Identity = 413/581 (71.08%), Postives = 477/581 (82.10%), Query Frame = 0

Query: 1   PSSSSSYSSSICNWKRQRPNNHKRSIIIQSALHSPSVLHFPNQNYHNKLSSLREDQDKPI 60
           PS SSS   S  N K +R     R +   S   +P++L+FPN    N +         P 
Sbjct: 26  PSPSSSV--SFTNTKPRR-----RKLSANSVSDTPNLLNFPNYPSPNPII--------PE 85

Query: 61  KDPN-WNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLT 120
           KD + WN  Q+AA+ A D  E+AL+ +E   PLPKT DPR QISGN+APVPE  VK +L+
Sbjct: 86  KDTSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLS 145

Query: 121 VAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLV 180
           V G IPDCI+GVYLRNGANPLFEP +GHHLFDGDGMVHAV I  G ASY+CRFTET RLV
Sbjct: 146 VDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLV 205

Query: 181 QERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLA 240
           QE++LG P+FPKAIGELHGHSGI RL+LFY RGLFGL++HKNGTGVANAGLV+F+DRLLA
Sbjct: 206 QEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLA 265

Query: 241 MSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKP 300
           MSEDDLPY VR+T +GDL+T+GR+DF+ QL  +S MIAHPK+DPV++EL+ALSYDVV KP
Sbjct: 266 MSEDDLPYQVRVTDNGDLETIGRFDFDGQL--SSAMIAHPKIDPVTKELFALSYDVVKKP 325

Query: 301 YLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIY 360
           YLKYF+FSP+G KSPDVEIPL +PTMMHDFAITENFVVIPDQQVVFKL +M  G SPV Y
Sbjct: 326 YLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKY 385

Query: 361 DKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIF 420
           D +K SRFGILP+NA DAS ++WVESPETFCFHLWNAWE P T+EVVVIGSCMTPADSIF
Sbjct: 386 DGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIF 445

Query: 421 NECDENLKSVLSEIRLNLRTGKSTRRAIMAEEEQVNLEAGMVNRNRLGRRTRFAYLAIAE 480
           NECDE L SVLSEIRLNL+TGKSTRR I+    Q+NLEAGMVNRN LGR+TR+AYLAIAE
Sbjct: 446 NECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAE 505

Query: 481 PWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNSAAEDDGYILAFVHDERTWK 540
           PWPKVSGFAKVDL+TG+VK H YG +KYGGEPFFLPR   S  EDDGYI++FVHDE +W+
Sbjct: 506 PWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWE 565

Query: 541 SELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           SEL IVNA+ L++EATVKLPSRVPYGFHGTFV+S D+ NQA
Sbjct: 566 SELHIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQA 589

BLAST of MS011023 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 832.4 bits (2149), Expect = 2.2e-241
Identity = 408/579 (70.47%), Postives = 469/579 (81.00%), Query Frame = 0

Query: 2   SSSSSYSSSICNWKRQRPNNHKRSIIIQSALHSPSVLHFPNQNYHNKLSSLREDQDKPIK 61
           SSS S   S C+      +   R + + SALH+P  LHFP Q+  N  + + + + K   
Sbjct: 25  SSSQSSDLSYCS-SLPMASRVTRKLNVSSALHTPPALHFPKQS-SNSPAIVVKPKAKESN 84

Query: 62  DPNWNFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLTVA 121
               N FQ+AAA A D  E  L+S E  HPLPKTADP VQI+GNFAPV E PV+ NL V 
Sbjct: 85  TKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVV 144

Query: 122 GTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLVQE 181
           G +PD I GVY+RNGANPL EP  GHH FDGDGMVHAV    GSASYACRFT+T+R VQE
Sbjct: 145 GKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQE 204

Query: 182 RELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLAMS 241
           R+LGRPVFPKAIGELHGH+GI RL+LFY R   G+VD  +GTGVANAGLV+FN RLLAMS
Sbjct: 205 RQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMS 264

Query: 242 EDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKPYL 301
           EDDLPY V+ITP+GDLKTVGR+DF+ QL   STMIAHPKVDP S EL+ALSYDVV KPYL
Sbjct: 265 EDDLPYQVQITPNGDLKTVGRFDFDGQL--ESTMIAHPKVDPESGELFALSYDVVSKPYL 324

Query: 302 KYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIYDK 361
           KYFRFSPDGTKSPDVEI L  PTMMHDFAITENFVV+PDQQVVFKL EM++GGSPV+YDK
Sbjct: 325 KYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYDK 384

Query: 362 DKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIFNE 421
           +K +RFGIL K A D+S I W+++P+ FCFHLWNAWEEP T+EVVVIGSCMTP DSIFNE
Sbjct: 385 NKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVVIGSCMTPPDSIFNE 444

Query: 422 CDENLKSVLSEIRLNLRTGKSTRRAIMA-EEEQVNLEAGMVNRNRLGRRTRFAYLAIAEP 481
            DENLKSVLSEIRLNL+TG+STRR I++ E++QVNLEAGMVNRN LGR+T+FAYLA+AEP
Sbjct: 445 SDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEP 504

Query: 482 WPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNSAAEDDGYILAFVHDERTWKS 541
           WPKVSGFAKVDL TG+VKKH+YGD +YGGEP FLP +     ED+GYIL FVHDE+TWKS
Sbjct: 505 WPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGE--GGEEDEGYILCFVHDEKTWKS 564

Query: 542 ELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQ 580
           ELQIVNA+ L+VEATVKLPSRVPYGFHGTF+ ++DL  Q
Sbjct: 565 ELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQ 597

BLAST of MS011023 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 820.8 bits (2119), Expect = 6.7e-238
Identity = 390/516 (75.58%), Postives = 452/516 (87.60%), Query Frame = 0

Query: 66  NFFQKAAAMAFDVVESALISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLTVAGTIP 125
           N FQKAAA+A D  E ALIS E   PLPKTADPRVQI+GN++PVPE  V+ NLTV GTIP
Sbjct: 70  NIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGTIP 129

Query: 126 DCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRLVQERELG 185
           DCI+GVY+RNGANP+FEPTAGHHLFDGDGMVHAV I  GSASYACRFT+T RLVQE+ LG
Sbjct: 130 DCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGSASYACRFTKTERLVQEKRLG 189

Query: 186 RPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLAMSEDDL 245
           RPVFPKAIGELHGHSGI RL+LFY RGL GL++++NG GVANAGLV+FN+RLLAMSEDDL
Sbjct: 190 RPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDDL 249

Query: 246 PYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKPYLKYFR 305
           PY ++IT +GDL+TVGRYDF+ QL   S MIAHPK+DPV++EL+ALSYDVV KPYLKYFR
Sbjct: 250 PYQLKITQTGDLQTVGRYDFDGQL--KSAMIAHPKLDPVTKELHALSYDVVKKPYLKYFR 309

Query: 306 FSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIYDKDKKS 365
           FSPDG KSP++EIPL+TPTM+HDFAITENFVVIPDQQVVFKLGEM+ G SPV++D +K S
Sbjct: 310 FSPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDGEKVS 369

Query: 366 RFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSIFNECDEN 425
           R GI+PK+A++AS IIWV SPETFCFHLWNAWE P TEE+VVIGSCM+PADSIFNE DE+
Sbjct: 370 RLGIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNERDES 429

Query: 426 LKSVLSEIRLNLRTGKSTRRAIMAEEEQVNLEAGMVNRNRLGRRTRFAYLAIAEPWPKVS 485
           L+SVLSEIR+NLRT K+TRR+++  E+ VNLE GMVNRNRLGR+TRFA+LAIA PWPKVS
Sbjct: 430 LRSVLSEIRINLRTRKTTRRSLLVNED-VNLEIGMVNRNRLGRKTRFAFLAIAYPWPKVS 489

Query: 486 GFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNSAA--EDDGYILAFVHDERTWKSELQ 545
           GFAKVDL TG++KK+IYG  KYGGEPFFLP +  +    EDDGYI   VHDE T  SELQ
Sbjct: 490 GFAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIFCHVHDEETKTSELQ 549

Query: 546 IVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQ 580
           I+NA++LK+EAT+KLPSRVPYGFHGTFVDSN+L +Q
Sbjct: 550 IINAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

BLAST of MS011023 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 788.1 bits (2034), Expect = 4.8e-228
Identity = 390/582 (67.01%), Postives = 462/582 (79.38%), Query Frame = 0

Query: 4   SSSYSSSICNWKRQRP----NNHKRSIIIQSALHSPSVLHFPNQNYHNKLSSLREDQDKP 63
           S S+SS++ N K   P    ++  +   + S+L SP+      +   N +++L       
Sbjct: 82  SLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPTFKPPSWKKLCNDVTNLIPKTTN- 141

Query: 64  IKDPNWNFFQKAAAMAFDVVESALISKE-LQHPLPKTADPRVQISGNFAPVPEHPVKHNL 123
            ++P  N  Q+ AAM  D VE+A+IS E  +HP PKTADP VQI+GNF PVPE PV HNL
Sbjct: 142 -QNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVPEKPVVHNL 201

Query: 124 TVAGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINGGSASYACRFTETHRL 183
            V GT+P+CI GVY+RNGANPL +P +GHHLFDGDGMVHAV  + GS SYACRFTET+RL
Sbjct: 202 PVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRFDNGSVSYACRFTETNRL 261

Query: 184 VQERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLL 243
           VQERE GRPVFPKAIGELHGH GI +L+LF  RGLFGLVD   G GVANAGLV+FN  LL
Sbjct: 262 VQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHLL 321

Query: 244 AMSEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHK 303
           AMSEDDLPYHV++T +GDL+T GRYDF+ QL   STMIAHPK+DP +REL+ALSYDVV K
Sbjct: 322 AMSEDDLPYHVKVTQTGDLETSGRYDFDGQL--KSTMIAHPKIDPETRELFALSYDVVSK 381

Query: 304 PYLKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVI 363
           PYLKYFRF+ DG KSPDVEIPL  PTM+HDFAITENFVVIPDQQVVF+L EM++GGSPV+
Sbjct: 382 PYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIRGGSPVV 441

Query: 364 YDKDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEEVVVIGSCMTPADSI 423
           YD+ KKSRFGIL KNA DAS I W+E P+ FCFHLWN+WEEP T+EVVVIGSCMTP DSI
Sbjct: 442 YDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIGSCMTPPDSI 501

Query: 424 FNECDENLKSVLSEIRLNLRTGKSTRRAIMAEEEQVNLEAGMVNRNRLGRRTRFAYLAIA 483
           FNE DE L+SVLSEIRLNL+TG+STRR +++  EQVNLEAGMVNRN LGR+TR+AYLA+ 
Sbjct: 502 FNEHDETLQSVLSEIRLNLKTGESTRRPVIS--EQVNLEAGMVNRNLLGRKTRYAYLALT 561

Query: 484 EPWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNSAAEDDGYILAFVHDERTW 543
           EPWPKVSGFAKVDL+TG+++K+IYG+ KYGGEP FLP       ED GYI+ FVHDE   
Sbjct: 562 EPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP--SGDGEEDGGYIMVFVHDEEKV 621

Query: 544 KSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQA 581
           KSELQ++NA+++K+EATV LPSRVPYGFHGTF+   DL  QA
Sbjct: 622 KSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQA 655

BLAST of MS011023 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 619.8 bits (1597), Expect = 2.3e-177
Identity = 306/523 (58.51%), Postives = 382/523 (73.04%), Query Frame = 0

Query: 63  PNWNFFQKAAAMAFDVVESAL-ISKELQHPLPKTADPRVQISGNFAPVPEHPVKHNLTVA 122
           PN N  QK AA   D +ES++ I  E   PLPK  DP VQ+SGNFAPV E PV++ L V 
Sbjct: 61  PNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQNGLEVV 120

Query: 123 GTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTIN-GGSASYACRFTETHRLVQ 182
           G IP C+ GVY+RNGANP+F P AGHHLFDGDGM+HAV+I      SY+CR+T+T+RLVQ
Sbjct: 121 GQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQ 180

Query: 183 ERELGRPVFPKAIGELHGHSGIGRLLLFYGRGLFGLVDHKNGTGVANAGLVFFNDRLLAM 242
           E  LGR VFPK IGELHGHSG+ RL LF  R   GLVD   G GVANAG+VFFN RLLAM
Sbjct: 181 ETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFNGRLLAM 240

Query: 243 SEDDLPYHVRITPSGDLKTVGRYDFNNQLNSNSTMIAHPKVDPVSRELYALSYDVVHKPY 302
           SEDDLPY V+I   GDL+T+GR+ F++Q+  +S++IAHPKVD  + +L+ LSY+V+ KP+
Sbjct: 241 SEDDLPYQVKIDGQGDLETIGRFGFDDQI--DSSVIAHPKVDATTGDLHTLSYNVLKKPH 300

Query: 303 LKYFRFSPDGTKSPDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLGEMVKGGSPVIYD 362
           L+Y +F+  G K+ DVEI L  PTM+HDFAITENFVVIPDQQ+VFKL EM++GGSPVIY 
Sbjct: 301 LRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYV 360

Query: 363 KDKKSRFGILPKNASDASGIIWVESPETFCFHLWNAWEEPATEE----VVVIGSCMTPAD 422
           K+K +RFG+L K     S I WV+ P+ FCFHLWNAWEE  TEE    +VVIGSCM+P D
Sbjct: 361 KEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEE-RTEEGDPVIVVIGSCMSPPD 420

Query: 423 SIFNECDENLKSVLSEIRLNLRTGKSTRRAIMAEEEQVNLEAGMVNRNRLGRRTRFAYLA 482
           +IF+E  E  +  LSEIRLN+RT +S R+ I+     VNLEAG +NR+ +GR+++F Y+A
Sbjct: 421 TIFSESGEPTRVELSEIRLNMRTKESNRKVIVT---GVNLEAGHINRSYVGRKSQFVYIA 480

Query: 483 IAEPWPKVSGFAKVDLATGQVKKHIYGDRKYGGEPFFLPRDPNSAAEDDGYILAFVHDER 542
           IA+PWPK SG AKVD+  G V +  YG  ++GGEP F+P       ED GY++ FV DE 
Sbjct: 481 IADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPE--GEGEEDKGYVMGFVRDEE 540

Query: 543 TWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDSNDLQNQ 580
             +SE  +V+A D+K  A V+LP RVPYGFHGTFV  N L+ Q
Sbjct: 541 KDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575