MS010861 (gene) Bitter gourd (TR) v1

Overview


Name	MS010861
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	heat stress transcription factor B-2a-like
Location	scaffold35: 2554495 .. 2555404 (-)
RNA-Seq Expression	MS010861
Synteny	MS010861

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGTCTTCAAGAACCAGAAGCCCTGCTCCTTTTCTATCCAAGACTTATGATCTGTTAGAAGAAGCTGGAAGCTTCCATTTTAAAGCAGCTCATCATGAAGAAGAAGAAGAAGAAAATGGTGGCAATCCTAGGATTGTGTCCTGGAATGCAGAGGGAAGTGGGTTCATAGTTTGGTCTCCTACTGAGTTCTCTGAGATTTTATTGCCCAAGTACTTCAAGCACAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACCTATGTATGTGATTCTCTCTCTCTCTTTCTCTCTCTGGGAATGCCCCACCATTGCTATTCCCATAAACATCCTGAAAGACGAGTTGATCATTTAGATCCAAGAGGCGCTTTTCCTAATTTTTTGAATACTGGTATTCGTCCTTAGAAATTGGATCTATACATATATTTTATCTATAATACATATTCTTCATACTCTAATCCTGTAGAATTCGAAGATACCATATTTAGTTACCACTAAATCGAAAAGCTTAAGATGATAGGTTACAATATTAGTAACCTTATCTTTCGGACGTCATTTTCTAGGAAGGGTTTCGATCTAGTTCTTGTGATTTTGGAGACAGGGCTTCAAGAAAACGTCGCCAAAGAGGTGGGAGTTCAAGCACGAGAAGTTTCAGCGGGGAAACAGGCACATGTTAGGGGAGATTGTACGTAAGAAGTGTGAACCAAGTGTGTTTCCAGCCTTTCTAAGATCGTCTCGTGAAGGGGATACAACAATAGTGGCTGCTGATCGGGAGAATGACGACCATATGTTACTAATGGAAGAGAACAAGAACCTAAGAAGACAGAAGCAGGAATTGCAGACAAAAATAGCCCAGTTCAAAGAACTCCATATTAGACTGCTGGACTGTCTCGGTCAGTGCATTGAGAAA

mRNA sequence

ATGCAGTCTTCAAGAACCAGAAGCCCTGCTCCTTTTCTATCCAAGACTTATGATCTGTTAGAAGAAGCTGGAAGCTTCCATTTTAAAGCAGCTCATCATGAAGAAGAAGAAGAAGAAAATGGTGGCAATCCTAGGATTGTGTCCTGGAATGCAGAGGGAAGTGGGTTCATAGTTTGGTCTCCTACTGAGTTCTCTGAGATTTTATTGCCCAAGTACTTCAAGCACAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACCTATGGCTTCAAGAAAACGTCGCCAAAGAGGTGGGAGTTCAAGCACGAGAAGTTTCAGCGGGGAAACAGGCACATGTTAGGGGAGATTGTACGTAAGAAGTGTGAACCAAGTGTGTTTCCAGCCTTTCTAAGATCGTCTCGTGAAGGGGATACAACAATAGTGGCTGCTGATCGGGAGAATGACGACCATATGTTACTAATGGAAGAGAACAAGAACCTAAGAAGACAGAAGCAGGAATTGCAGACAAAAATAGCCCAGTTCAAAGAACTCCATATTAGACTGCTGGACTGTCTCGGTCAGTGCATTGAGAAA

Coding sequence (CDS)

ATGCAGTCTTCAAGAACCAGAAGCCCTGCTCCTTTTCTATCCAAGACTTATGATCTGTTAGAAGAAGCTGGAAGCTTCCATTTTAAAGCAGCTCATCATGAAGAAGAAGAAGAAGAAAATGGTGGCAATCCTAGGATTGTGTCCTGGAATGCAGAGGGAAGTGGGTTCATAGTTTGGTCTCCTACTGAGTTCTCTGAGATTTTATTGCCCAAGTACTTCAAGCACAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACCTATGGCTTCAAGAAAACGTCGCCAAAGAGGTGGGAGTTCAAGCACGAGAAGTTTCAGCGGGGAAACAGGCACATGTTAGGGGAGATTGTACGTAAGAAGTGTGAACCAAGTGTGTTTCCAGCCTTTCTAAGATCGTCTCGTGAAGGGGATACAACAATAGTGGCTGCTGATCGGGAGAATGACGACCATATGTTACTAATGGAAGAGAACAAGAACCTAAGAAGACAGAAGCAGGAATTGCAGACAAAAATAGCCCAGTTCAAAGAACTCCATATTAGACTGCTGGACTGTCTCGGTCAGTGCATTGAGAAA

Protein sequence

MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWSPTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKKCEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFKELHIRLLDCLGQCIEK

Homology

[Close]

BLAST of MS010861 vs. NCBI nr
Match: XP_022142291.1 (heat stress transcription factor B-2a-like [Momordica charantia])

HSP 1 Score: 391.0 bits (1003), Expect = 6.0e-105
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWS 60
           MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWS
Sbjct: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWS 60

Query: 61  PTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKK 120
           PTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKK
Sbjct: 61  PTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKK 120

Query: 121 CEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFKELHIR 180
           CEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFKELHIR
Sbjct: 121 CEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFKELHIR 180

Query: 181 LLDCLGQCIEK 192
           LLDCLGQCIEK
Sbjct: 181 LLDCLGQCIEK 191

[Close]

BLAST of MS010861 vs. NCBI nr
Match: TYK13134.1 (heat stress transcription factor B-2a-like [Cucumis melo var. makuwa])

HSP 1 Score: 321.6 bits (823), Expect = 4.5e-84
Identity = 165/196 (84.18%), Postives = 173/196 (88.27%), Query Frame = 0


Query: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAA-----HHEEEEEENGGNPRIVSWNAEGSG 60
           MQSSRTR+PAPFLSKTYDLLEEAGSFHFK A       EEEEEENGGN RIVSWNAEGSG
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEEEEEEENGGN-RIVSWNAEGSG 60

Query: 61  FIVWSPTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGE 120
           FIVWSP +FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG RHML E
Sbjct: 61  FIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHMLVE 120

Query: 121 IVRKKCEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFK 180
           IVRKKCEPSVFPAFLRSS EG  T +A ++EN DH+LLMEEN NLRRQK ELQ +IAQFK
Sbjct: 121 IVRKKCEPSVFPAFLRSSHEGGAT-MAVNQENGDHLLLMEENNNLRRQKLELQAQIAQFK 180

Query: 181 ELHIRLLDCLGQCIEK 192
            LHIRLLDCLGQCIEK
Sbjct: 181 ALHIRLLDCLGQCIEK 194

[Close]

BLAST of MS010861 vs. NCBI nr
Match: XP_008439862.1 (PREDICTED: heat stress transcription factor B-2a-like [Cucumis melo] >KAA0052690.1 heat stress transcription factor B-2a-like [Cucumis melo var. makuwa])

HSP 1 Score: 320.5 bits (820), Expect = 1.0e-83
Identity = 165/200 (82.50%), Postives = 173/200 (86.50%), Query Frame = 0


Query: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAA---------HHEEEEEENGGNPRIVSWNA 60
           MQSSRTR+PAPFLSKTYDLLEEAGSFHFK A           EEEEEENGGN RIVSWNA
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEDEEEEEEEEENGGN-RIVSWNA 60

Query: 61  EGSGFIVWSPTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRH 120
           EGSGFIVWSP +FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG RH
Sbjct: 61  EGSGFIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRH 120

Query: 121 MLGEIVRKKCEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKI 180
           ML EIVRKKCEPSVFPAFLRSS EG  T +A ++EN DH+LLMEEN NLRRQK ELQ +I
Sbjct: 121 MLVEIVRKKCEPSVFPAFLRSSHEGGAT-MAVNQENGDHLLLMEENNNLRRQKLELQAQI 180

Query: 181 AQFKELHIRLLDCLGQCIEK 192
           AQFK LHIRLLDCLGQCIEK
Sbjct: 181 AQFKALHIRLLDCLGQCIEK 198

[Close]

BLAST of MS010861 vs. NCBI nr
Match: XP_023543806.1 (heat stress transcription factor B-2a-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 319.3 bits (817), Expect = 2.2e-83
Identity = 161/198 (81.31%), Postives = 173/198 (87.37%), Query Frame = 0


Query: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEE-------EENGGNPRIVSWNAEG 60
           MQSSR R+PAPFLSKTYDLL+EAGSFHFKAA  E+EE       EENGG  RIVSWNA+G
Sbjct: 5   MQSSRIRNPAPFLSKTYDLLQEAGSFHFKAADEEDEEDEEDEEAEENGGGNRIVSWNADG 64

Query: 61  SGFIVWSPTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHML 120
           SGFIVWSP +FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHML
Sbjct: 65  SGFIVWSPADFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHML 124

Query: 121 GEIVRKKCEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQ 180
            EIVRKKCEPSVFPAFLRSS EG  T +  D+EN DH+LLMEENKNLRRQK ELQ +IAQ
Sbjct: 125 VEIVRKKCEPSVFPAFLRSSHEGAAT-MGVDQENGDHLLLMEENKNLRRQKLELQAQIAQ 184

Query: 181 FKELHIRLLDCLGQCIEK 192
           FK LHIRLLDC+G+ IEK
Sbjct: 185 FKALHIRLLDCVGRRIEK 201

[Close]

BLAST of MS010861 vs. NCBI nr
Match: XP_022925809.1 (heat stress transcription factor B-2a-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 317.8 bits (813), Expect = 6.5e-83
Identity = 160/195 (82.05%), Postives = 173/195 (88.72%), Query Frame = 0


Query: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHE----EEEEENGGNPRIVSWNAEGSGF 60
           MQSSR R+PAPFLSKTYDLL+EAGSFHFKAA  E    EE+EENGG  RIVSWNA+GSGF
Sbjct: 5   MQSSRIRNPAPFLSKTYDLLQEAGSFHFKAADEEDEEDEEDEENGGGNRIVSWNADGSGF 64

Query: 61  IVWSPTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEI 120
           IVWSP +FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNR ML EI
Sbjct: 65  IVWSPADFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRRMLVEI 124

Query: 121 VRKKCEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFKE 180
           VRKKCEPSVFPA+LRSS EG  T +A D+EN DH+LLMEENKNLRRQK ELQ +IAQFK 
Sbjct: 125 VRKKCEPSVFPAYLRSSHEGAAT-MAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKA 184

Query: 181 LHIRLLDCLGQCIEK 192
           LHIRLLDC+G+ IEK
Sbjct: 185 LHIRLLDCVGRRIEK 198

[Close]

BLAST of MS010861 vs. ExPASy Swiss-Prot
Match: Q9SCW4 (Heat stress transcription factor B-2a OS=Arabidopsis thaliana OX=3702 GN=HSFB2A PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.3e-29
Identity = 75/203 (36.95%), Postives = 109/203 (53.69%), Query Frame = 0


Query: 3   SSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWSPT 62
           SS+   P PFL+KT++L+E++                      ++SWN +GS FIVW+PT
Sbjct: 15  SSQRSIPTPFLTKTFNLVEDSSI------------------DDVISWNEDGSSFIVWNPT 74

Query: 63  EFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKKCE 122
           +F++ LLPK+FKHNNFSSF+RQLNTYGFKK  P RWEF ++ F+RG + +L EI R+K  
Sbjct: 75  DFAKDLLPKHFKHNNFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKIT 134

Query: 123 PSVFPAFLRSSREGDTTIVAA------DRENDDHML----------------------LM 178
            +       SS + + T+V +      D  N+  M                       L+
Sbjct: 135 TTHQTVVAPSSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELL 194

[Close]

BLAST of MS010861 vs. ExPASy Swiss-Prot
Match: Q6VBB2 (Heat stress transcription factor A-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA2B PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.8e-27
Identity = 70/183 (38.25%), Postives = 100/183 (54.64%), Query Frame = 0


Query: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWS 60
           M+      P PFL+KTYD++++AG+                     VSW+A  + F+VW 
Sbjct: 42  MEGLHDAGPPPFLTKTYDMVDDAGT------------------DAAVSWSATSNSFVVWD 101

Query: 61  PTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKK 120
           P  F+ +LLP++FKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG RH+L  I R+K
Sbjct: 102 PHAFATVLLPRFFKHNNFSSFVRQLNTYGFRKVDPDRWEFANENFLRGQRHLLKNIKRRK 161

Query: 121 -------CEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQ 177
                   + S+ P +L     G    +  DR   D  LLM E   LR+++Q  +  +  
Sbjct: 162 PPSHTASNQQSLGP-YLEVGHFGYDAEI--DRLKRDKQLLMAEVVKLRQEQQNTKANLKA 203

[Close]

BLAST of MS010861 vs. ExPASy Swiss-Prot
Match: Q9LQM7 (Heat stress transcription factor A-1d OS=Arabidopsis thaliana OX=3702 GN=HSFA1D PE=1 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 4.0e-27
Identity = 76/193 (39.38%), Postives = 105/193 (54.40%), Query Frame = 0


Query: 6   TRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWSPTEFS 65
           + +P PFLSKTYD++++         H+ +          IVSW+A  + FIVW P EF+
Sbjct: 32  SNAPPPFLSKTYDMVDD---------HNTDS---------IVSWSANNNSFIVWKPPEFA 91

Query: 66  EILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKKCEPSV 125
             LLPK FKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG +H+L  I R+K     
Sbjct: 92  RDLLPKNFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKPAHGQ 151

Query: 126 FPAFLRSSR-EGDTTIVAA-------------DRENDDHMLLMEENKNLRRQKQ----EL 181
                RS    G  + V+A             +R   D  +LM+E   LR+Q+Q    +L
Sbjct: 152 GQGHQRSQHSNGQNSSVSACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQL 206

[Close]

BLAST of MS010861 vs. ExPASy Swiss-Prot
Match: Q93VB5 (Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4D PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 5.3e-27
Identity = 58/112 (51.79%), Postives = 74/112 (66.07%), Query Frame = 0


Query: 9   PAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWSPTEFSEIL 68
           P PFL KTY+++E+A + H                  +VSW   G+ F+VW+P +FS  L
Sbjct: 18  PPPFLIKTYEMVEDAATNH------------------VVSWGPGGASFVVWNPLDFSRDL 77

Query: 69  LPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKK 121
           LPKYFKHNNFSSFIRQLNTYGF+K  P+RWEF +E F RG+ H+L  I R+K
Sbjct: 78  LPKYFKHNNFSSFIRQLNTYGFRKIDPERWEFANEDFIRGHTHLLKNIHRRK 111

[Close]

BLAST of MS010861 vs. ExPASy Swiss-Prot
Match: P41151 (Heat stress transcription factor A-1a OS=Arabidopsis thaliana OX=3702 GN=HSFA1A PE=1 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 1.2e-26
Identity = 69/173 (39.88%), Postives = 93/173 (53.76%), Query Frame = 0


Query: 9   PAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWSPTEFSEIL 68
           P PFLSKTYD++E+  +                    IVSW+   + FIVW P EFS  L
Sbjct: 50  PPPFLSKTYDMVEDPAT------------------DAIVSWSPTNNSFIVWDPPEFSRDL 109

Query: 69  LPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKKC-----EP 128
           LPKYFKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG +H+L +I R+K        
Sbjct: 110 LPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSS 169

Query: 129 SVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFKE 177
           S  P   + S+   +    +         L EE + L+R K  L  ++ + ++
Sbjct: 170 SSNPQSQQLSQGQGSMAALSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQ 204

[Close]

BLAST of MS010861 vs. ExPASy TrEMBL
Match: A0A6J1CLR9 (heat stress transcription factor B-2a-like OS=Momordica charantia OX=3673 GN=LOC111012446 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.9e-105
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWS 60
           MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWS
Sbjct: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWS 60

Query: 61  PTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKK 120
           PTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKK
Sbjct: 61  PTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKK 120

Query: 121 CEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFKELHIR 180
           CEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFKELHIR
Sbjct: 121 CEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFKELHIR 180

Query: 181 LLDCLGQCIEK 192
           LLDCLGQCIEK
Sbjct: 181 LLDCLGQCIEK 191

[Close]

BLAST of MS010861 vs. ExPASy TrEMBL
Match: A0A5D3CQ35 (Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007440 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.2e-84
Identity = 165/196 (84.18%), Postives = 173/196 (88.27%), Query Frame = 0


Query: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAA-----HHEEEEEENGGNPRIVSWNAEGSG 60
           MQSSRTR+PAPFLSKTYDLLEEAGSFHFK A       EEEEEENGGN RIVSWNAEGSG
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEEEEEEENGGN-RIVSWNAEGSG 60

Query: 61  FIVWSPTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGE 120
           FIVWSP +FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG RHML E
Sbjct: 61  FIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHMLVE 120

Query: 121 IVRKKCEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFK 180
           IVRKKCEPSVFPAFLRSS EG  T +A ++EN DH+LLMEEN NLRRQK ELQ +IAQFK
Sbjct: 121 IVRKKCEPSVFPAFLRSSHEGGAT-MAVNQENGDHLLLMEENNNLRRQKLELQAQIAQFK 180

Query: 181 ELHIRLLDCLGQCIEK 192
            LHIRLLDCLGQCIEK
Sbjct: 181 ALHIRLLDCLGQCIEK 194

[Close]

BLAST of MS010861 vs. ExPASy TrEMBL
Match: A0A5A7UBZ5 (Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003020 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.9e-84
Identity = 165/200 (82.50%), Postives = 173/200 (86.50%), Query Frame = 0


Query: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAA---------HHEEEEEENGGNPRIVSWNA 60
           MQSSRTR+PAPFLSKTYDLLEEAGSFHFK A           EEEEEENGGN RIVSWNA
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEDEEEEEEEEENGGN-RIVSWNA 60

Query: 61  EGSGFIVWSPTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRH 120
           EGSGFIVWSP +FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG RH
Sbjct: 61  EGSGFIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRH 120

Query: 121 MLGEIVRKKCEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKI 180
           ML EIVRKKCEPSVFPAFLRSS EG  T +A ++EN DH+LLMEEN NLRRQK ELQ +I
Sbjct: 121 MLVEIVRKKCEPSVFPAFLRSSHEGGAT-MAVNQENGDHLLLMEENNNLRRQKLELQAQI 180

Query: 181 AQFKELHIRLLDCLGQCIEK 192
           AQFK LHIRLLDCLGQCIEK
Sbjct: 181 AQFKALHIRLLDCLGQCIEK 198

[Close]

BLAST of MS010861 vs. ExPASy TrEMBL
Match: A0A1S3AZS2 (heat stress transcription factor B-2a-like OS=Cucumis melo OX=3656 GN=LOC103484524 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.9e-84
Identity = 165/200 (82.50%), Postives = 173/200 (86.50%), Query Frame = 0


Query: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAA---------HHEEEEEENGGNPRIVSWNA 60
           MQSSRTR+PAPFLSKTYDLLEEAGSFHFK A           EEEEEENGGN RIVSWNA
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEDEEEEEEEEENGGN-RIVSWNA 60

Query: 61  EGSGFIVWSPTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRH 120
           EGSGFIVWSP +FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG RH
Sbjct: 61  EGSGFIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRH 120

Query: 121 MLGEIVRKKCEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKI 180
           ML EIVRKKCEPSVFPAFLRSS EG  T +A ++EN DH+LLMEEN NLRRQK ELQ +I
Sbjct: 121 MLVEIVRKKCEPSVFPAFLRSSHEGGAT-MAVNQENGDHLLLMEENNNLRRQKLELQAQI 180

Query: 181 AQFKELHIRLLDCLGQCIEK 192
           AQFK LHIRLLDCLGQCIEK
Sbjct: 181 AQFKALHIRLLDCLGQCIEK 198

[Close]

BLAST of MS010861 vs. ExPASy TrEMBL
Match: A0A6J1ED85 (heat stress transcription factor B-2a-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433105 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.1e-83
Identity = 160/195 (82.05%), Postives = 173/195 (88.72%), Query Frame = 0


Query: 1   MQSSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHE----EEEEENGGNPRIVSWNAEGSGF 60
           MQSSR R+PAPFLSKTYDLL+EAGSFHFKAA  E    EE+EENGG  RIVSWNA+GSGF
Sbjct: 5   MQSSRIRNPAPFLSKTYDLLQEAGSFHFKAADEEDEEDEEDEENGGGNRIVSWNADGSGF 64

Query: 61  IVWSPTEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEI 120
           IVWSP +FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNR ML EI
Sbjct: 65  IVWSPADFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRRMLVEI 124

Query: 121 VRKKCEPSVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFKE 180
           VRKKCEPSVFPA+LRSS EG  T +A D+EN DH+LLMEENKNLRRQK ELQ +IAQFK 
Sbjct: 125 VRKKCEPSVFPAYLRSSHEGAAT-MAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKA 184

Query: 181 LHIRLLDCLGQCIEK 192
           LHIRLLDC+G+ IEK
Sbjct: 185 LHIRLLDCVGRRIEK 198

[Close]

BLAST of MS010861 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 129.4 bits (324), Expect = 3.1e-30
Identity = 75/203 (36.95%), Postives = 109/203 (53.69%), Query Frame = 0


Query: 3   SSRTRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWSPT 62
           SS+   P PFL+KT++L+E++                      ++SWN +GS FIVW+PT
Sbjct: 15  SSQRSIPTPFLTKTFNLVEDSSI------------------DDVISWNEDGSSFIVWNPT 74

Query: 63  EFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKKCE 122
           +F++ LLPK+FKHNNFSSF+RQLNTYGFKK  P RWEF ++ F+RG + +L EI R+K  
Sbjct: 75  DFAKDLLPKHFKHNNFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKIT 134

Query: 123 PSVFPAFLRSSREGDTTIVAA------DRENDDHML----------------------LM 178
            +       SS + + T+V +      D  N+  M                       L+
Sbjct: 135 TTHQTVVAPSSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELL 194

[Close]

BLAST of MS010861 vs. TAIR 10
Match: AT1G32330.1 (heat shock transcription factor A1D )

HSP 1 Score: 122.9 bits (307), Expect = 2.9e-28
Identity = 76/193 (39.38%), Postives = 105/193 (54.40%), Query Frame = 0


Query: 6   TRSPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWSPTEFS 65
           + +P PFLSKTYD++++         H+ +          IVSW+A  + FIVW P EF+
Sbjct: 32  SNAPPPFLSKTYDMVDD---------HNTDS---------IVSWSANNNSFIVWKPPEFA 91

Query: 66  EILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKKCEPSV 125
             LLPK FKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG +H+L  I R+K     
Sbjct: 92  RDLLPKNFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKPAHGQ 151

Query: 126 FPAFLRSSR-EGDTTIVAA-------------DRENDDHMLLMEENKNLRRQKQ----EL 181
                RS    G  + V+A             +R   D  +LM+E   LR+Q+Q    +L
Sbjct: 152 GQGHQRSQHSNGQNSSVSACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQL 206

[Close]

BLAST of MS010861 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 121.3 bits (303), Expect = 8.3e-28
Identity = 69/173 (39.88%), Postives = 93/173 (53.76%), Query Frame = 0


Query: 9   PAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWSPTEFSEIL 68
           P PFLSKTYD++E+  +                    IVSW+   + FIVW P EFS  L
Sbjct: 50  PPPFLSKTYDMVEDPAT------------------DAIVSWSPTNNSFIVWDPPEFSRDL 109

Query: 69  LPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKKC-----EP 128
           LPKYFKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG +H+L +I R+K        
Sbjct: 110 LPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSS 169

Query: 129 SVFPAFLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFKE 177
           S  P   + S+   +    +         L EE + L+R K  L  ++ + ++
Sbjct: 170 SSNPQSQQLSQGQGSMAALSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQ 204

[Close]

BLAST of MS010861 vs. TAIR 10
Match: AT4G13980.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 120.6 bits (301), Expect = 1.4e-27
Identity = 65/168 (38.69%), Postives = 96/168 (57.14%), Query Frame = 0


Query: 9   PAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWSPTEFSEIL 68
           PAPFL KTY++++++ +                   +IVSW+A  + FIVW+  EFS +L
Sbjct: 21  PAPFLVKTYEMVDDSST------------------DQIVSWSANNNSFIVWNHAEFSRLL 80

Query: 69  LPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKKCEPSVFPA 128
           LP YFKHNNFSSFIRQLNTYGF+K  P+RWEF ++ F +  +H+L  I R+K      P 
Sbjct: 81  LPTYFKHNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRK------PI 140

Query: 129 FLRSSREGDTTIVAADRENDDHMLLMEENKNLRRQKQELQTKIAQFKE 177
              S     +T       + +  +L E+   L R+K  ++ K+ +FK+
Sbjct: 141 HSHSHPPASST-------DQERAVLQEQMDKLSREKAAIEAKLLKFKQ 157

[Close]

BLAST of MS010861 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 120.2 bits (300), Expect = 1.9e-27
Identity = 69/174 (39.66%), Postives = 99/174 (56.90%), Query Frame = 0


Query: 8   SPAPFLSKTYDLLEEAGSFHFKAAHHEEEEEENGGNPRIVSWNAEGSGFIVWSPTEFSEI 67
           S  PFLSKTYD++++  +                    +VSW++  + F+VWS  EFS++
Sbjct: 24  SVPPFLSKTYDMVDDPLT------------------NEVVSWSSGNNSFVVWSAPEFSKV 83

Query: 68  LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRHMLGEIVRKK---CEPS 127
           LLPKYFKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG + +L  IVR+K    + +
Sbjct: 84  LLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQN 143

Query: 128 VFPAFLRSSREGDTTIVA-------ADRENDDHMLLMEENKNLRRQKQELQTKI 172
                ++SS  G    V         +R   D  +LM+E   LR+Q+Q  + ++
Sbjct: 144 QQQTQVQSSSVGACVEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQL 179

The following BLAST results are available for this feature:

BLAST of MS010861 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142291.1	6.0e-105	100.00	heat stress transcription factor B-2a-like [Momordica charantia]	[more]
TYK13134.1	4.5e-84	84.18	heat stress transcription factor B-2a-like [Cucumis melo var. makuwa]	[more]
XP_008439862.1	1.0e-83	82.50	PREDICTED: heat stress transcription factor B-2a-like [Cucumis melo] >KAA0052690...	[more]
XP_023543806.1	2.2e-83	81.31	heat stress transcription factor B-2a-like isoform X1 [Cucurbita pepo subsp. pep...	[more]
XP_022925809.1	6.5e-83	82.05	heat stress transcription factor B-2a-like isoform X1 [Cucurbita moschata]	[more]

BLAST of MS010861 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SCW4	4.3e-29	36.95	Heat stress transcription factor B-2a OS=Arabidopsis thaliana OX=3702 GN=HSFB2A ...	[more]
Q6VBB2	1.8e-27	38.25	Heat stress transcription factor A-2b OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q9LQM7	4.0e-27	39.38	Heat stress transcription factor A-1d OS=Arabidopsis thaliana OX=3702 GN=HSFA1D ...	[more]
Q93VB5	5.3e-27	51.79	Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
P41151	1.2e-26	39.88	Heat stress transcription factor A-1a OS=Arabidopsis thaliana OX=3702 GN=HSFA1A ...	[more]

BLAST of MS010861 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CLR9	2.9e-105	100.00	heat stress transcription factor B-2a-like OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A5D3CQ35	2.2e-84	84.18	Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A5A7UBZ5	4.9e-84	82.50	Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3AZS2	4.9e-84	82.50	heat stress transcription factor B-2a-like OS=Cucumis melo OX=3656 GN=LOC1034845...	[more]
A0A6J1ED85	3.1e-83	82.05	heat stress transcription factor B-2a-like isoform X1 OS=Cucurbita moschata OX=3...	[more]

BLAST of MS010861 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62020.1	3.1e-30	36.95	heat shock transcription factor B2A	[more]
AT1G32330.1	2.9e-28	39.38	heat shock transcription factor A1D	[more]
AT4G17750.1	8.3e-28	39.88	heat shock factor 1	[more]
AT4G13980.1	1.4e-27	38.69	winged-helix DNA-binding transcription factor family protein	[more]
AT5G16820.1	1.9e-27	39.66	heat shock factor 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 143..177
None	No IPR available	PANTHER	PTHR10015:SF308	HEAT STRESS TRANSCRIPTION FACTOR B-2A-LIKE	coord: 3..190
IPR000232	Heat shock factor (HSF)-type, DNA-binding	SMART	SM00415	hsfneu3	coord: 8..119 e-value: 1.5E-37 score: 140.8
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PFAM	PF00447	HSF_DNA-bind	coord: 12..119 e-value: 2.5E-28 score: 98.5
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 4..120 e-value: 7.7E-31 score: 108.6
IPR027725	Heat shock transcription factor family	PANTHER	PTHR10015	HEAT SHOCK TRANSCRIPTION FACTOR	coord: 3..190
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 40..119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010861.1	MS010861.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding