MS010831 (gene) Bitter gourd (TR) v1

Overview


Name	MS010831
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Aspergillus nuclease S(1)
Location	scaffold35: 2306415 .. 2309253 (+)
RNA-Seq Expression	MS010831
Synteny	MS010831

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCCGGTTTGAGCTCTTCTGGAGAGCTTCCGTTTTTCTGCTACTTGTACCTGGAATCCTCGGCTGGGGAAAGGAAGGTCACTATGCAATCTGCAAGATAGCCGAGGTAAGAATATTCAGTTCATTTTTAAGCATCAGTCATCATTTATTTTGTCAAGCTTTCGGTGTTACAGAAGGGTAATAGGGAATATGGTGCAGTCCAGTTCAGAAAATAAGGATAATAACTGGGGTTGTTGATTGAAAGTCCTCGAAAGTGTAGCTTTAATAGTGTAGGTCAGGATTTAAGGTAAAGCAAACGTGGCAAAGGAGAGAGGACCTTGTGAAATTTGGTCCGCTATTAGAAAACTTTGGACCCATTTGGATATTTGACATATAGAACTGAAATAAACACTTGTATCCACTATTTTAGCTTCTTAGTAAGCTAGAAATCTGTTTGGTGAGACGACTTGGAGCTGGTTGTATCTGATATCATCCTATGATAGGGAGTGCAATCATTAGATCCTGCAGTGTTCAAGACAAGACTGATATCACTTTTCTGGTTTAGGGATATTTCACTGAAGATGCTCTATCCATGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGTGATCTCGCCGCTGTATGCTCCTGGCCTGATGAGATTCGACGAAAGGCTCATTATCGCTGGAGTAGCGTTTTACACTATGTTGACACTCCTGATTTCTTTTGTAATTATAATTACTCGAGTAAGTTTGAGCTTAAGGTATTTTCAGTTCCTTTTGTGAACTATCATGCCGTTTTACTGTTTTATATAATGGTTGTTATTGTAGTGTTTATGAGCTTTAAAATTCAAAAGCATTGTTGAACCACAGGAATACTGAGGCTGAGAAATTTGATAGTTCATGATAACATAACCAATGTATCCTCTGAAGTTAACTTATATGGCCAATGGCGTAATTATTATAGCACTAGCGCCAATATCTTCCTAAATTAAAAATTTCATATTCATAAGAACATAAGATTTTGACACAGAATTTCAATGTCTCGTTGCTTCAAGATGATAAAGTGTTCACATGAAATGAACTAACTTGTTCTGCAATTCTTGTGCCAGTTGCCTGTTGCCTTTTGTTCCCCTTCTAGCTTTGAAACATCTTATGTTGCCAACTACGAAGTTAATGAAAATATGTCATTCTGAACATGTCATATATGATGGTTATTAAATTCAATTTGATTTTTGATTCCTTTCATTCAACAAGGTATTTTTAGTTGCAAGATTTAACTCTAATGATTGATTTTCTCTTCATGACAATGTTTACGAGTGTAACAGTTTCCTCTGCTTCATTATAGGAGACTGTCATGACACTCATGGACATAACGGTAGGTGTGTGATTGCGGCAATTTACAACTATACCATGCAACTTGAATCAACTTACAAAGAGATGACTTCAGATGTTAGATGTAAGCACCGTAAATAACTACCCATCCTGTCCATACATTTTTCGAACAAACGTCATTTTTATTCACGTGGTATTTTCAGATAACTTAACAGAGGCTCTCATGTTCTTGTCCCATTTTATTGGAGATGTCCACCAGGTTTGCCCTTTCTACAGATCTGAACTTTTAGGCACTCAGAGAAACTGAAAATATGAAGTGCTAGGTTCATGAAACTTATATTATGTAACTACAAACTTTAGTCCTTGGAATATTGTTGAAAGTTTATTTTCATCCGATGGTAAAATCACTCTTTCTTTTCAAATAGGAATCTTTATAGTTTGATTAGCAAGTTTGTGCATTAACTACATAACAATACTAATAGTGTCAACTGTATTTCATTTTTTTCCCTAAAGAATCGAATAATTTATGATTTTATTCCTTTGTATCTGAATTTTCCGTTATTTGGTGGCAGCCCCTGCATGTTGGTTTCATTGGAGATCTTGGTGGTAATTCAATAACAGTCCGTTGGTACCGTAGAAAGACTAATCTTCATCATGTAAGTGTCACCTAGAGATTTGAACTCCCTCCTCACAAGTACCCTCCTTATTTCATCACTATTCTTCATACACCTTTAACCATTTGACAGTAAACACACGAAACATCTCGTAAAACTTCACCATGATGGTTAAAACTACAAGCTCGGAAATCCCATTACATACTGCAGAATGTTTTGGTTTTTATCCATCTGGTATTGGAATAGTTTTGTCCATAAAAATGAAATGGTCTTTCTACAGGTATGGGATAACATGATCATTGAGTCTGCCTTGAAGACGTTCTACAATTCAAGCCTTGCACTAATGATTCAAGCCATTCAAAATAGCATTGTGGTGTGCCTCCTTTCTCTGATTTTATGTTCTGACTTGCCTTTTTTGATAGAACAATTTACTTTTCTTATTTTTTACTCATAGTTCTTTTTCCTTATGCCATCTTCGTTTTAAATTTCTGAAGGATGAATGGTCTAATGATGTATCAAGTTGGAGAAATTGCACAGAGAACCACACAGTTTGTCCTAACCCGTGAGTATTTTATCATTCTTATCCCTTATCCATGTTCGGCAACCTGTTTATGTTCAAATCATGTAATCAAATGATTCCTCTGCTGTCATATGAGCATCCCTGATTAGTATACGAAGATGGACTTGCCACTATTATACGTTGCTGAAACTTAAGATAACGTCTTCTTTAGCTCGAAAGAGGAGAAAAATATTCATCGGGTGAAAAATAACCTTCATTTTCCCACCATAAAGTTCTAGGAAATGGTAAGACTTGATTGGATGCCTCTTATTTTTCAGGTATGCTTCTGAAAGTATTAGCTTGGCGTGTAAATATGCGTACAAGAATGCCACTCCCGGAAGCACACTAGAT

mRNA sequence

ATGGGCCGGTTTGAGCTCTTCTGGAGAGCTTCCGTTTTTCTGCTACTTGTACCTGGAATCCTCGGCTGGGGAAAGGAAGGTCACTATGCAATCTGCAAGATAGCCGAGGGATATTTCACTGAAGATGCTCTATCCATGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGTGATCTCGCCGCTGTATGCTCCTGGCCTGATGAGATTCGACGAAAGGCTCATTATCGCTGGAGTAGCGTTTTACACTATGTTGACACTCCTGATTTCTTTTGTAATTATAATTACTCGAGTAATTTCCTCTGCTTCATTATAGGAGACTGTCATGACACTCATGGACATAACGGTAGGTGTGTGATTGCGGCAATTTACAACTATACCATGCAACTTGAATCAACTTACAAAGAGATGACTTCAGATGTTAGATATAACTTAACAGAGGCTCTCATGTTCTTGTCCCATTTTATTGGAGATGTCCACCAGCCCCTGCATGTTGGTTTCATTGGAGATCTTGGTGGTAATTCAATAACAGTCCGTTGGTACCGTAGAAAGACTAATCTTCATCATGTATGGGATAACATGATCATTGAGTCTGCCTTGAAGACGTTCTACAATTCAAGCCTTGCACTAATGATTCAAGCCATTCAAAATAGCATTGTGGATGAATGGTCTAATGATGTATCAAGTTGGAGAAATTGCACAGAGAACCACACAGTTTGTCCTAACCCGTATGCTTCTGAAAGTATTAGCTTGGCGTGTAAATATGCGTACAAGAATGCCACTCCCGGAAGCACACTAGAT

Coding sequence (CDS)

ATGGGCCGGTTTGAGCTCTTCTGGAGAGCTTCCGTTTTTCTGCTACTTGTACCTGGAATCCTCGGCTGGGGAAAGGAAGGTCACTATGCAATCTGCAAGATAGCCGAGGGATATTTCACTGAAGATGCTCTATCCATGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGTGATCTCGCCGCTGTATGCTCCTGGCCTGATGAGATTCGACGAAAGGCTCATTATCGCTGGAGTAGCGTTTTACACTATGTTGACACTCCTGATTTCTTTTGTAATTATAATTACTCGAGTAATTTCCTCTGCTTCATTATAGGAGACTGTCATGACACTCATGGACATAACGGTAGGTGTGTGATTGCGGCAATTTACAACTATACCATGCAACTTGAATCAACTTACAAAGAGATGACTTCAGATGTTAGATATAACTTAACAGAGGCTCTCATGTTCTTGTCCCATTTTATTGGAGATGTCCACCAGCCCCTGCATGTTGGTTTCATTGGAGATCTTGGTGGTAATTCAATAACAGTCCGTTGGTACCGTAGAAAGACTAATCTTCATCATGTATGGGATAACATGATCATTGAGTCTGCCTTGAAGACGTTCTACAATTCAAGCCTTGCACTAATGATTCAAGCCATTCAAAATAGCATTGTGGATGAATGGTCTAATGATGTATCAAGTTGGAGAAATTGCACAGAGAACCACACAGTTTGTCCTAACCCGTATGCTTCTGAAAGTATTAGCTTGGCGTGTAAATATGCGTACAAGAATGCCACTCCCGGAAGCACACTAGAT

Protein sequence

MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCVIAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTVCPNPYASESISLACKYAYKNATPGSTLD

Homology

[Close]

BLAST of MS010831 vs. NCBI nr
Match: XP_022142132.1 (endonuclease 4-like [Momordica charantia])

HSP 1 Score: 535.4 bits (1378), Expect = 2.8e-148
Identity = 255/266 (95.86%), Postives = 255/266 (95.86%), Query Frame = 0


Query: 1   MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLA 60
           MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLA
Sbjct: 1   MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLA 60

Query: 61  AVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCVIA 120
           AVCSWPDEIRRKAHY WSSVLHYVDTPDFFCNYNYS         DCHDTHGHNGRCVIA
Sbjct: 61  AVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSR--------DCHDTHGHNGRCVIA 120

Query: 121 AIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWY 180
           AIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWY
Sbjct: 121 AIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTVCP 240
           RRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEW NDVSSWRNCTENHTVCP
Sbjct: 181 RRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLD 267
           NPYASESISLACKYAYKNATPGSTLD
Sbjct: 241 NPYASESISLACKYAYKNATPGSTLD 258

[Close]

BLAST of MS010831 vs. NCBI nr
Match: XP_038883248.1 (endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883249.1 endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883250.1 endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883251.1 endonuclease 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 489.2 bits (1258), Expect = 2.3e-134
Identity = 230/268 (85.82%), Postives = 244/268 (91.04%), Query Frame = 0


Query: 1   MGRFELFWRAS--VFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60
           MG+ +L W A+  VFLLL+PGILGWGKEGHY ICKIAEGYFTED LS VKELLPASAEGD
Sbjct: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCV 120
           LAAVCSWPDEI+RKAHYRWSS LHYVDTPDFFCNYN SS    +  GDCHDTHGH GRCV
Sbjct: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSSKLKLY--GDCHDTHGHKGRCV 120

Query: 121 IAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVR 180
            AAIYNYTMQLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVR
Sbjct: 121 TAAIYNYTMQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVR 180

Query: 181 WYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTV 240
           WYRRKTNLHHVWDNMIIESALKTFY+S+L LMIQAIQN+I DEW NDVS+WRNCT NHT 
Sbjct: 181 WYRRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTA 240

Query: 241 CPNPYASESISLACKYAYKNATPGSTLD 267
           CPNPYASESISLACKYAYKNATPGSTL+
Sbjct: 241 CPNPYASESISLACKYAYKNATPGSTLE 266

[Close]

BLAST of MS010831 vs. NCBI nr
Match: XP_038883252.1 (endonuclease 4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 483.0 bits (1242), Expect = 1.6e-132
Identity = 228/268 (85.07%), Postives = 241/268 (89.93%), Query Frame = 0


Query: 1   MGRFELFWRAS--VFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60
           MG+ +L W A+  VFLLL+PGILGWGKEGHY ICKIAEGYFTED LS VKELLPASAEGD
Sbjct: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCV 120
           LAAVCSWPDEI+RKAHYRWSS LHYVDTPDFFCNYN S         DCHDTHGH GRCV
Sbjct: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSR--------DCHDTHGHKGRCV 120

Query: 121 IAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVR 180
            AAIYNYTMQLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVR
Sbjct: 121 TAAIYNYTMQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVR 180

Query: 181 WYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTV 240
           WYRRKTNLHHVWDNMIIESALKTFY+S+L LMIQAIQN+I DEW NDVS+WRNCT NHT 
Sbjct: 181 WYRRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTA 240

Query: 241 CPNPYASESISLACKYAYKNATPGSTLD 267
           CPNPYASESISLACKYAYKNATPGSTL+
Sbjct: 241 CPNPYASESISLACKYAYKNATPGSTLE 260

[Close]

BLAST of MS010831 vs. NCBI nr
Match: XP_023543506.1 (endonuclease 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 473.8 bits (1218), Expect = 9.9e-130
Identity = 226/268 (84.33%), Postives = 236/268 (88.06%), Query Frame = 0


Query: 1   MGRFE--LFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60
           MG+ E  L  +A  FLLLVPGILGWGKEGHY ICKIAEGYFTED LSMVKELLPASAEGD
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCV 120
           LAAVCSWPDEIR KAHYRWSS LHYVDTPDFFCNYNYS         DCHDTHGH GRCV
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSR--------DCHDTHGHKGRCV 120

Query: 121 IAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVR 180
            AAIYNYT QLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVR
Sbjct: 121 TAAIYNYTRQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVR 180

Query: 181 WYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTV 240
           WYRRKTNLHHVWDNMIIESALKTFY+S+L LMIQAIQ +I  EW NDVS+WRNC  NHT 
Sbjct: 181 WYRRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTA 240

Query: 241 CPNPYASESISLACKYAYKNATPGSTLD 267
           CPNPYASESISLACKYAYKNATPGSTL+
Sbjct: 241 CPNPYASESISLACKYAYKNATPGSTLE 260

[Close]

BLAST of MS010831 vs. NCBI nr
Match: XP_022950165.1 (endonuclease 4-like [Cucurbita moschata])

HSP 1 Score: 473.8 bits (1218), Expect = 9.9e-130
Identity = 225/268 (83.96%), Postives = 236/268 (88.06%), Query Frame = 0


Query: 1   MGRFE--LFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60
           MG+FE  L  +A  FLLLVPGILGWGKEGHY ICKIAEGYFTED LSMVKELLPASAEGD
Sbjct: 1   MGQFELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCV 120
           LAAVCSWPDEIR KAHYRWSS LHYVDTPDFFCNYNYS         DCHDTHGH GRCV
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSR--------DCHDTHGHKGRCV 120

Query: 121 IAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVR 180
            AAIYNYT QLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVR
Sbjct: 121 TAAIYNYTRQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVR 180

Query: 181 WYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTV 240
           WYRRKTNLHHVWDNMIIESALKTFY+S+L LMIQAIQ +I  EW NDVS+WRNC  NHT 
Sbjct: 181 WYRRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTA 240

Query: 241 CPNPYASESISLACKYAYKNATPGSTLD 267
           CPNPYASESI LACKYAY+NATPGSTL+
Sbjct: 241 CPNPYASESIRLACKYAYRNATPGSTLE 260

[Close]

BLAST of MS010831 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 2.4e-102
Identity = 172/259 (66.41%), Postives = 202/259 (77.99%), Query Frame = 0


Query: 7   FWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLAAVCSWP 66
           F R  V   L+ G L WGKEGHY +CKIAE YF E+ ++ VK+LLP SA+GDLA+VCSWP
Sbjct: 9   FARVLVLTQLINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWP 68

Query: 67  DEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCVIAAIYNYT 126
           DEI+    +RW+S LHYVDTPD+ CNY Y          DCHDTH +  RCV  AI+NYT
Sbjct: 69  DEIKHHWQWRWTSPLHYVDTPDYRCNYEYCR--------DCHDTHKNQDRCVTGAIFNYT 128

Query: 127 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 186
           MQL S  +   + V YNLTEALMFLSHFIGD+HQPLHVGF+GD GGN+ITVRWYRRKTNL
Sbjct: 129 MQLMSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNL 188

Query: 187 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTVCPNPYASE 246
           HHVWDNMIIESALKT+YN SL LMI+A+Q ++ ++WSNDV  W +C  N T CPNPYASE
Sbjct: 189 HHVWDNMIIESALKTYYNKSLPLMIEALQANLTNDWSNDVPLWESCQLNQTACPNPYASE 248

Query: 247 SISLACKYAYKNATPGSTL 266
           SI+LACKYAY+NATPG+TL
Sbjct: 249 SINLACKYAYRNATPGTTL 259

[Close]

BLAST of MS010831 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 1.6e-90
Identity = 155/261 (59.39%), Postives = 194/261 (74.33%), Query Frame = 0


Query: 8   WRASVFLL--LVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAE-GDLAAVCS 67
           W  SV +L  LV G L WGK+GHY +CK+AEG+F +D ++ VK+LLP S + G LA  CS
Sbjct: 4   WIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFCS 63

Query: 68  WPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCVIAAIYN 127
           WPDEI++ + ++W+S LHYV+TP++ CNY Y          DCHDTH H   CV  AI+N
Sbjct: 64  WPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCR--------DCHDTHKHKDWCVTGAIFN 123

Query: 128 YTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKT 187
           YT QL S  +   + V YNLTEAL+FLSH++GDVHQPLH GF+GDLGGN+I V WY  K+
Sbjct: 124 YTNQLMSASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKS 183

Query: 188 NLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTVCPNPYA 247
           NLHHVWDNMII+SAL+T+YNSSL  MIQA+Q  + + WSNDV SW++C  +   CPN YA
Sbjct: 184 NLHHVWDNMIIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYA 243

Query: 248 SESISLACKYAYKNATPGSTL 266
           SESI LACKYAY+NATPG+TL
Sbjct: 244 SESIDLACKYAYRNATPGTTL 256

[Close]

BLAST of MS010831 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 4.4e-88
Identity = 159/267 (59.55%), Postives = 188/267 (70.41%), Query Frame = 0


Query: 1   MGRFELFWRASVFLL--LVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60
           MG     W  S+ +L  LV G L WG  GHYA+CKIA+ YF ED +  VK+LLP SA G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 61  LAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCV 120
           LAAVCSWPDEI++   +RW+S LH+ DTPD+ CNY YS         DC         CV
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSR--------DC-----PKDWCV 120

Query: 121 IAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVR 180
             AI+NYT QL ST +   S V YNLTEALMFLSH++GD+HQPLH GFIGDLGGN I V 
Sbjct: 121 TGAIFNYTNQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVH 180

Query: 181 WYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTV 240
           WY ++TNLH VWD+MIIESAL+T+YNSSL  MI  +Q  + + WSNDV SW +C  N T 
Sbjct: 181 WYNQETNLHRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTA 240

Query: 241 CPNPYASESISLACKYAYKNATPGSTL 266
           CPNPYASESI LACKYAY+NAT G+TL
Sbjct: 241 CPNPYASESIDLACKYAYRNATAGTTL 254

[Close]

BLAST of MS010831 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 3.4e-72
Identity = 133/253 (52.57%), Postives = 164/253 (64.82%), Query Frame = 0


Query: 14  LLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLAAVCSWPDEIRRKA 73
           L   P I GWGKEGH  ICKIA+    E A   VKELLP SAEGDL+++C W D +  K 
Sbjct: 19  LYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLWADRV--KF 78

Query: 74  HYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCVIAAIYNYTMQLESTY 133
            Y WSS LHY++TPD  C+Y Y+         DC D  G  GRCV  AIYNYT QL S  
Sbjct: 79  RYHWSSPLHYINTPD-ACSYQYNR--------DCKDESGEKGRCVAGAIYNYTTQLLSYK 138

Query: 134 KEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHHVWDNM 193
              +S  +YNLTEAL+F+SHF+GD+HQPLHV +  D GGN+I V WY RK NLHH+WD+ 
Sbjct: 139 TAASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSN 198

Query: 194 IIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTVCPNPYASESISLACK 253
           IIE+A    YNS+L  M+ A++ +I  EW++ V  W  CT+  T CP+ YASE I  AC 
Sbjct: 199 IIETAEADLYNSALEGMVDALKKNITTEWADQVKRWETCTKK-TACPDIYASEGIQAACD 258

Query: 254 YAYKNATPGSTLD 267
           +AYK  T G TL+
Sbjct: 259 WAYKGVTEGDTLE 259

[Close]

BLAST of MS010831 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.6e-69
Identity = 133/268 (49.63%), Postives = 172/268 (64.18%), Query Frame = 0


Query: 8   WRASVFLLLVPGIL---------GWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 67
           +R+S  L+LV GIL          W KEGH   C+IA+         +V+ LLP   +GD
Sbjct: 5   FRSSTRLILVLGILILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGD 64

Query: 68  LAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCV 127
           L+A+C WPD+IR    YRW+S LHY+DTPD  C+Y YS         DCHD HG    CV
Sbjct: 65  LSALCVWPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSR--------DCHDQHGLKDMCV 124

Query: 128 IAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVR 187
             AI N+T QL+  Y E TSD RYN+TEAL+FLSHF+GD+HQP+HVGF  D GGN+I +R
Sbjct: 125 DGAIQNFTSQLQH-YGEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLR 184

Query: 188 WYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDE-WSNDVSSWRNCTENHT 247
           WY+ K+NLHHVWD  II +ALK  Y+ +L L+ + ++ +I +  W +D+SSW  C +   
Sbjct: 185 WYKHKSNLHHVWDREIILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECND-LI 244

Query: 248 VCPNPYASESISLACKYAYKNATPGSTL 266
            CP+ YASESI LACK+ YK    G TL
Sbjct: 245 ACPHKYASESIKLACKWGYKGVKSGETL 262

[Close]

BLAST of MS010831 vs. ExPASy TrEMBL
Match: A0A6J1CLU4 (Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111012332 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.3e-148
Identity = 255/266 (95.86%), Postives = 255/266 (95.86%), Query Frame = 0


Query: 1   MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLA 60
           MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLA
Sbjct: 1   MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLA 60

Query: 61  AVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCVIA 120
           AVCSWPDEIRRKAHY WSSVLHYVDTPDFFCNYNYS         DCHDTHGHNGRCVIA
Sbjct: 61  AVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSR--------DCHDTHGHNGRCVIA 120

Query: 121 AIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWY 180
           AIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWY
Sbjct: 121 AIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTVCP 240
           RRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEW NDVSSWRNCTENHTVCP
Sbjct: 181 RRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLD 267
           NPYASESISLACKYAYKNATPGSTLD
Sbjct: 241 NPYASESISLACKYAYKNATPGSTLD 258

[Close]

BLAST of MS010831 vs. ExPASy TrEMBL
Match: A0A6J1GEX6 (Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111453339 PE=3 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 4.8e-130
Identity = 225/268 (83.96%), Postives = 236/268 (88.06%), Query Frame = 0


Query: 1   MGRFE--LFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60
           MG+FE  L  +A  FLLLVPGILGWGKEGHY ICKIAEGYFTED LSMVKELLPASAEGD
Sbjct: 1   MGQFELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCV 120
           LAAVCSWPDEIR KAHYRWSS LHYVDTPDFFCNYNYS         DCHDTHGH GRCV
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSR--------DCHDTHGHKGRCV 120

Query: 121 IAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVR 180
            AAIYNYT QLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVR
Sbjct: 121 TAAIYNYTRQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVR 180

Query: 181 WYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTV 240
           WYRRKTNLHHVWDNMIIESALKTFY+S+L LMIQAIQ +I  EW NDVS+WRNC  NHT 
Sbjct: 181 WYRRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTA 240

Query: 241 CPNPYASESISLACKYAYKNATPGSTLD 267
           CPNPYASESI LACKYAY+NATPGSTL+
Sbjct: 241 CPNPYASESIRLACKYAYRNATPGSTLE 260

[Close]

BLAST of MS010831 vs. ExPASy TrEMBL
Match: A0A6J1IVA4 (Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111478715 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.0e-129
Identity = 225/268 (83.96%), Postives = 235/268 (87.69%), Query Frame = 0


Query: 1   MGRFE--LFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60
           MG+ E  L  +A  FLLLVPGILGWGKEGHY ICKIAEGYFTED LSMVKELLPASAEGD
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCV 120
           LAAVCSWPDEIR KAHYRWSS LHYVDTPDFFCNYNYS         DCHDTH H GRCV
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSR--------DCHDTHEHKGRCV 120

Query: 121 IAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVR 180
            AAIYNYT QLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVR
Sbjct: 121 TAAIYNYTRQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVR 180

Query: 181 WYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTV 240
           WYRRKTNLHHVWDNMIIESALKTFY+S+L LMIQAIQ +I  EW NDVS+WRNC  NHT 
Sbjct: 181 WYRRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTA 240

Query: 241 CPNPYASESISLACKYAYKNATPGSTLD 267
           CPNPYASESISLACKYAYKNATPGSTL+
Sbjct: 241 CPNPYASESISLACKYAYKNATPGSTLE 260

[Close]

BLAST of MS010831 vs. ExPASy TrEMBL
Match: A0A1S3CEU3 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 8.2e-122
Identity = 211/268 (78.73%), Postives = 231/268 (86.19%), Query Frame = 0


Query: 1   MGRFELFWRAS--VFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60
           MG+ EL W A+  VFLLL+PGILGWG+EGHYA+CKIAEGY TEDALSMV+ELLPASAEGD
Sbjct: 21  MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 80

Query: 61  LAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCV 120
           LAAVCSW DE+R    Y WSSVLH+VDTPDFFCNYN S         DCHD+H H GRCV
Sbjct: 81  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSR--------DCHDSHRHKGRCV 140

Query: 121 IAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVR 180
            AAIYNYTMQLES YKE+TS+VRYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGN I V 
Sbjct: 141 TAAIYNYTMQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNLIKVG 200

Query: 181 WYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTV 240
           WY+R TNLHHVWD MIIESALK FY+S+L LMIQAIQ++I DEW N+VS+WRNCT NHT 
Sbjct: 201 WYQRSTNLHHVWDTMIIESALKRFYHSNLMLMIQAIQSNISDEWHNEVSAWRNCTGNHTA 260

Query: 241 CPNPYASESISLACKYAYKNATPGSTLD 267
           CPNPYASESISLACKYAYKNATPGSTL+
Sbjct: 261 CPNPYASESISLACKYAYKNATPGSTLE 280

[Close]

BLAST of MS010831 vs. ExPASy TrEMBL
Match: A0A1S3CF84 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 8.2e-122
Identity = 211/268 (78.73%), Postives = 231/268 (86.19%), Query Frame = 0


Query: 1   MGRFELFWRAS--VFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60
           MG+ EL W A+  VFLLL+PGILGWG+EGHYA+CKIAEGY TEDALSMV+ELLPASAEGD
Sbjct: 1   MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 60

Query: 61  LAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCV 120
           LAAVCSW DE+R    Y WSSVLH+VDTPDFFCNYN S         DCHD+H H GRCV
Sbjct: 61  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSR--------DCHDSHRHKGRCV 120

Query: 121 IAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVR 180
            AAIYNYTMQLES YKE+TS+VRYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGN I V 
Sbjct: 121 TAAIYNYTMQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNLIKVG 180

Query: 181 WYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTV 240
           WY+R TNLHHVWD MIIESALK FY+S+L LMIQAIQ++I DEW N+VS+WRNCT NHT 
Sbjct: 181 WYQRSTNLHHVWDTMIIESALKRFYHSNLMLMIQAIQSNISDEWHNEVSAWRNCTGNHTA 240

Query: 241 CPNPYASESISLACKYAYKNATPGSTLD 267
           CPNPYASESISLACKYAYKNATPGSTL+
Sbjct: 241 CPNPYASESISLACKYAYKNATPGSTLE 260

[Close]

BLAST of MS010831 vs. TAIR 10
Match: AT4G21585.1 (endonuclease 4 )

HSP 1 Score: 373.2 bits (957), Expect = 1.7e-103
Identity = 172/259 (66.41%), Postives = 202/259 (77.99%), Query Frame = 0


Query: 7   FWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLAAVCSWP 66
           F R  V   L+ G L WGKEGHY +CKIAE YF E+ ++ VK+LLP SA+GDLA+VCSWP
Sbjct: 9   FARVLVLTQLINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWP 68

Query: 67  DEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCVIAAIYNYT 126
           DEI+    +RW+S LHYVDTPD+ CNY Y          DCHDTH +  RCV  AI+NYT
Sbjct: 69  DEIKHHWQWRWTSPLHYVDTPDYRCNYEYCR--------DCHDTHKNQDRCVTGAIFNYT 128

Query: 127 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 186
           MQL S  +   + V YNLTEALMFLSHFIGD+HQPLHVGF+GD GGN+ITVRWYRRKTNL
Sbjct: 129 MQLMSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNL 188

Query: 187 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTVCPNPYASE 246
           HHVWDNMIIESALKT+YN SL LMI+A+Q ++ ++WSNDV  W +C  N T CPNPYASE
Sbjct: 189 HHVWDNMIIESALKTYYNKSLPLMIEALQANLTNDWSNDVPLWESCQLNQTACPNPYASE 248

Query: 247 SISLACKYAYKNATPGSTL 266
           SI+LACKYAY+NATPG+TL
Sbjct: 249 SINLACKYAYRNATPGTTL 259

[Close]

BLAST of MS010831 vs. TAIR 10
Match: AT4G21600.1 (endonuclease 5 )

HSP 1 Score: 334.0 bits (855), Expect = 1.1e-91
Identity = 155/261 (59.39%), Postives = 194/261 (74.33%), Query Frame = 0


Query: 8   WRASVFLL--LVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAE-GDLAAVCS 67
           W  SV +L  LV G L WGK+GHY +CK+AEG+F +D ++ VK+LLP S + G LA  CS
Sbjct: 4   WIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFCS 63

Query: 68  WPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCVIAAIYN 127
           WPDEI++ + ++W+S LHYV+TP++ CNY Y          DCHDTH H   CV  AI+N
Sbjct: 64  WPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCR--------DCHDTHKHKDWCVTGAIFN 123

Query: 128 YTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKT 187
           YT QL S  +   + V YNLTEAL+FLSH++GDVHQPLH GF+GDLGGN+I V WY  K+
Sbjct: 124 YTNQLMSASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKS 183

Query: 188 NLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTVCPNPYA 247
           NLHHVWDNMII+SAL+T+YNSSL  MIQA+Q  + + WSNDV SW++C  +   CPN YA
Sbjct: 184 NLHHVWDNMIIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYA 243

Query: 248 SESISLACKYAYKNATPGSTL 266
           SESI LACKYAY+NATPG+TL
Sbjct: 244 SESIDLACKYAYRNATPGTTL 256

[Close]

BLAST of MS010831 vs. TAIR 10
Match: AT4G21590.1 (endonuclease 3 )

HSP 1 Score: 325.9 bits (834), Expect = 3.1e-89
Identity = 159/267 (59.55%), Postives = 188/267 (70.41%), Query Frame = 0


Query: 1   MGRFELFWRASVFLL--LVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60
           MG     W  S+ +L  LV G L WG  GHYA+CKIA+ YF ED +  VK+LLP SA G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 61  LAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCV 120
           LAAVCSWPDEI++   +RW+S LH+ DTPD+ CNY YS         DC         CV
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSR--------DC-----PKDWCV 120

Query: 121 IAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVR 180
             AI+NYT QL ST +   S V YNLTEALMFLSH++GD+HQPLH GFIGDLGGN I V 
Sbjct: 121 TGAIFNYTNQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVH 180

Query: 181 WYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTV 240
           WY ++TNLH VWD+MIIESAL+T+YNSSL  MI  +Q  + + WSNDV SW +C  N T 
Sbjct: 181 WYNQETNLHRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTA 240

Query: 241 CPNPYASESISLACKYAYKNATPGSTL 266
           CPNPYASESI LACKYAY+NAT G+TL
Sbjct: 241 CPNPYASESIDLACKYAYRNATAGTTL 254

[Close]

BLAST of MS010831 vs. TAIR 10
Match: AT4G21590.2 (endonuclease 3 )

HSP 1 Score: 325.9 bits (834), Expect = 3.1e-89
Identity = 159/267 (59.55%), Postives = 188/267 (70.41%), Query Frame = 0


Query: 1   MGRFELFWRASVFLL--LVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60
           MG     W  S+ +L  LV G L WG  GHYA+CKIA+ YF ED +  VK+LLP SA G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 61  LAAVCSWPDEIRRKAHYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCV 120
           LAAVCSWPDEI++   +RW+S LH+ DTPD+ CNY YS         DC         CV
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSR--------DC-----PKDWCV 120

Query: 121 IAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVR 180
             AI+NYT QL ST +   S V YNLTEALMFLSH++GD+HQPLH GFIGDLGGN I V 
Sbjct: 121 TGAIFNYTNQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVH 180

Query: 181 WYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTV 240
           WY ++TNLH VWD+MIIESAL+T+YNSSL  MI  +Q  + + WSNDV SW +C  N T 
Sbjct: 181 WYNQETNLHRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTA 240

Query: 241 CPNPYASESISLACKYAYKNATPGSTL 266
           CPNPYASESI LACKYAY+NAT G+TL
Sbjct: 241 CPNPYASESIDLACKYAYRNATAGTTL 254

[Close]

BLAST of MS010831 vs. TAIR 10
Match: AT1G68290.1 (endonuclease 2 )

HSP 1 Score: 273.1 bits (697), Expect = 2.4e-73
Identity = 133/253 (52.57%), Postives = 164/253 (64.82%), Query Frame = 0


Query: 14  LLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLAAVCSWPDEIRRKA 73
           L   P I GWGKEGH  ICKIA+    E A   VKELLP SAEGDL+++C W D +  K 
Sbjct: 19  LYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLWADRV--KF 78

Query: 74  HYRWSSVLHYVDTPDFFCNYNYSSNFLCFIIGDCHDTHGHNGRCVIAAIYNYTMQLESTY 133
            Y WSS LHY++TPD  C+Y Y+         DC D  G  GRCV  AIYNYT QL S  
Sbjct: 79  RYHWSSPLHYINTPD-ACSYQYNR--------DCKDESGEKGRCVAGAIYNYTTQLLSYK 138

Query: 134 KEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHHVWDNM 193
              +S  +YNLTEAL+F+SHF+GD+HQPLHV +  D GGN+I V WY RK NLHH+WD+ 
Sbjct: 139 TAASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSN 198

Query: 194 IIESALKTFYNSSLALMIQAIQNSIVDEWSNDVSSWRNCTENHTVCPNPYASESISLACK 253
           IIE+A    YNS+L  M+ A++ +I  EW++ V  W  CT+  T CP+ YASE I  AC 
Sbjct: 199 IIETAEADLYNSALEGMVDALKKNITTEWADQVKRWETCTKK-TACPDIYASEGIQAACD 258

Query: 254 YAYKNATPGSTLD 267
           +AYK  T G TL+
Sbjct: 259 WAYKGVTEGDTLE 259

The following BLAST results are available for this feature:

BLAST of MS010831 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142132.1	2.8e-148	95.86	endonuclease 4-like [Momordica charantia]	[more]
XP_038883248.1	2.3e-134	85.82	endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883249.1 endonuclease ...	[more]
XP_038883252.1	1.6e-132	85.07	endonuclease 4-like isoform X2 [Benincasa hispida]	[more]
XP_023543506.1	9.9e-130	84.33	endonuclease 4-like [Cucurbita pepo subsp. pepo]	[more]
XP_022950165.1	9.9e-130	83.96	endonuclease 4-like [Cucurbita moschata]	[more]

BLAST of MS010831 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JJL0	2.4e-102	66.41	Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1	[more]
F4JJL3	1.6e-90	59.39	Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1	[more]
Q8LDW6	4.4e-88	59.55	Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1	[more]
Q9C9G4	3.4e-72	52.57	Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1	[more]
Q9SXA6	1.6e-69	49.63	Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1	[more]

BLAST of MS010831 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CLU4	1.3e-148	95.86	Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111012332 PE=3 SV...	[more]
A0A6J1GEX6	4.8e-130	83.96	Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111453339 PE=3 SV=...	[more]
A0A6J1IVA4	4.0e-129	83.96	Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111478715 PE=3 SV=1	[more]
A0A1S3CEU3	8.2e-122	78.73	Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1	[more]
A0A1S3CF84	8.2e-122	78.73	Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1	[more]

BLAST of MS010831 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21585.1	1.7e-103	66.41	endonuclease 4	[more]
AT4G21600.1	1.1e-91	59.39	endonuclease 5	[more]
AT4G21590.1	3.1e-89	59.55	endonuclease 3	[more]
AT4G21590.2	3.1e-89	59.55	endonuclease 3	[more]
AT1G68290.1	2.4e-73	52.57	endonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008947	Phospholipase C/P1 nuclease domain superfamily	GENE3D	1.10.575.10	P1 Nuclease	coord: 23..266 e-value: 1.5E-85 score: 289.2
IPR008947	Phospholipase C/P1 nuclease domain superfamily	SUPERFAMILY	48537	Phospholipase C/P1 nuclease	coord: 23..265
IPR003154	S1/P1 nuclease	PFAM	PF02265	S1-P1_nuclease	coord: 23..265 e-value: 2.3E-64 score: 217.6
IPR003154	S1/P1 nuclease	PANTHER	PTHR33146	ENDONUCLEASE 4	coord: 7..265
IPR003154	S1/P1 nuclease	CDD	cd11010	S1-P1_nuclease	coord: 23..264 e-value: 1.13356E-68 score: 210.704

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010831.1	MS010831.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006308	DNA catabolic process
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds
molecular_function	GO:0003676	nucleic acid binding