MS010820 (gene) Bitter gourd (TR) v1

Overview
NameMS010820
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionF-box domain-containing protein
Locationscaffold35: 2219603 .. 2222484 (-)
RNA-Seq ExpressionMS010820
SyntenyMS010820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTCGAAGAGGAAGAAGAAAGAGTCGGGTGACTCGGACGAGTCGAGTTTCCCAGATGAAGTTTTGGAACGAGTTCTGAGCTTGGTTAAGTCCCACAAAGACAGAAGCTCTGTTTCTCTGGTTTGTAAGGAATGGTACAACGCCGAGCGCTGGTCCCGGACCCACGTCTTCATCGGAAACTGCTACTCTGTCTCGCCGGAAATTGTAATCCGCCGGTTCCCCAACATCCGGAGCGTCACCATTAAAGGGAAGCCCCGGTTCTCGGATTTCAATCTCGTGCCTCCCAATTGGGGAGCCGATATCCATTCCTGGCTCCTTGCCTTCGCTTCCAAATCCCCAATTCTCGAAGAATTGAGGCTCAAGAGGATGGCCGTCACAGACGAGAGCTTGGAATTTCTGGCTCGTTCCTTCCCAAATTTCAAATCCATTTCACTCATGAGCTGCGATGGCTTCAGCACCGATGGGCTTGCCGCCATTGCCACACACTGCAAGTACGTCTTCGTTTAATTCCTTCTTCCTTTCTCTTCCATTTAAAACTGTGATTCGTTAGAACCAGATCTGGGTTGTTCTTGGAAAAGGGATTTCTTTTTTCTTTCTGAGATGATGTATTGATTTCTTTTTGCGTTAGATTCTGGGGTTTCTGTTGTGGTTATGAAATTAAAGCAGTATTTGGTTTGGTTTGGTCACTGATTCGTTTCTTTGAATTTGCTGCTTGCTTTTATATGGGGTTGCTCCCAACAGTATCATGTCGGAATCATACTGAAAAAGTTTTGAACTTTATTCATTTATACGAAAAAGAAGAAATGGAACTCATAATAAATACGATTGTTGGTACTGCGTATAATTCTGTTATGGTACCTATTATAGCATCACTGCTTCTGCTGATTTAAACTTTGATGAGAAATTTTCTCCAACTTTTGGCTCTCTCCACCTCTCATTTTCTGTTCCCCTTTTTTCTTGGATGCGACATGTAACCTGCTTTTCTCTGTCATATGGTCGTTGGCATATCACATCCTTGCAACTTTGTCTCCATTATTATCAAGTGGGTCATTAATTAATTAGTGATGATTTGGGAAGATCAGCCATTTGAAAAAGTCTGGCCTTCTCCCATTTTTTTTTTTCCTTTTTTTACAGGAATCTTCAACCTACTGCCATGGCTCCCCACTGATTTGCCAGCTCCCCAAATCTGTAGTTTCTTTAAAGAAGCTTCAATGGGGGGAAAGAATGTGCCCTTTTCTTTGAGGCCACCGTTTGTGCAATAAATTCTGGAATTTATGACATGTCCAGATGTAGACATGTAAATTAAGAGCGATCTTTTTGGACAGATTCTTAAAAAAGTAGAAAACAACTGAATGTGTGTTTTCAAAATGTGTTCTAAATAATTCTTGGGAAAGACATCTGAAGAAAATGTTTGTTTCTTGCGTGGTAGGAAGTTGACTGATCTTGCCCTACGAGTCCTCAGGTTTTTCTGTTTTTGCCTTTTTTGATCTCTTGTTTTTGCCTTTTGGTGACAGGAACTTGACGGAGCTTGACATACTTGAGAATGACATTATTGACAAAAGTGGAAATTGGCTGAGTTGCTTCCCGGATACATTGAAATCTCTGGAAGTACTAAATTTTGCCAGTTTGAACAGCGATGTCAGTTTCGAGGCCCTCGAGAAGCTGGTAAGAAGGTGCAAATCATTGAAGGTTTTGAAGGTTAATAGGAACATAAACTTGGAACAGTTGCAGAGGCTGCTTGTTCACGTTCCGCAGTTAACTGAGTTAGGCACGGGCTCTTTTTTGCAAAAGATCACTCTTCGGCAGTACTGTGACCTCGAAGATGCGTTTAAGAACTGCAAGAATCTGCATACCCTCTCTGGTTTGTTGGAGTCGACGGTGTTGTATCTCCAAGTTCTGTTTCCTGCCTGCACGAATTTAACTTTCTTGAATCTGAGCTACGCAACTCTGCATGGTGGCGAACTTGCAGGACTTCTTTCCCACTGTCCCGTTCTTCGTCGCCTTTGGGTACGACCCGTTTTCCCCTTCCTTTCTTTTGTTCAAGCACATCGGTGATGAGATATCTCCTTCTGTAACTGTTTACCATTGATTTGAATACTTCAGCTTCTAATATCAGGTTCATTGTGCAGGTTTTAGACACAGTTGAAGACAAGGGCCTGAAGGCTGTTGGAATAAGTTGCCCCTTGCTAGAGGAACTCCGTGTATTTCCAGCGGATCCGTATGCTGACAATCTAGTTCACGGTGTAACCGAGTCTGGGTTTCTTGCTGTCTCTTATGGCTGCCGTAAACTCCATTACGTTCTCTACTTCTGTCGCCAGATGACGAATGAGGCTGTAGCCACCATTGTGCAGAACTGCCCAGATTTCACCCATTTTCGTCTCTGCATCATGAACCCTGCCCAACCAGACTATCTCACAAATCAATCAATGGACGAGGCCTTTGGTGCTGTGGTGAAAACTTGCTCGAAACTACGTAGACTTGCCATTTCAGGCTTGCTAACGGACTTAGCATTTGAATACATCGGTAAATATGCGAAAAACTTGGAAACTCTTTCAGTGGCTTTTGCTGGAAAGAGCGACTATGGAATGCAGTGTGTTATGAATGGTTGTCCAAAGCTGAGGAAACTGGAAATTAGAGACAGCCCCTTTGGAAATGCTGCATTGCTTTCGGGTATGGAGAGGTACGAATCCATGAGATCGCTATGGATGTCTGCCTGCAAGGTGACAAGGAATGGGTGCCGAATTCTGGCAAAGCAGGTGCCTAGGTTGAATGTCGAGGTCATCAAGGGTGACAACTGCGGGAGTGATGAGGCTGAAAGCGTTTACGTATATCGCTCTGTCGCTGGTCCAAGAAAAGACGCCCCATCCTGCGTCCTCACCCTC

mRNA sequence

AATTCGAAGAGGAAGAAGAAAGAGTCGGGTGACTCGGACGAGTCGAGTTTCCCAGATGAAGTTTTGGAACGAGTTCTGAGCTTGGTTAAGTCCCACAAAGACAGAAGCTCTGTTTCTCTGGTTTGTAAGGAATGGTACAACGCCGAGCGCTGGTCCCGGACCCACGTCTTCATCGGAAACTGCTACTCTGTCTCGCCGGAAATTGTAATCCGCCGGTTCCCCAACATCCGGAGCGTCACCATTAAAGGGAAGCCCCGGTTCTCGGATTTCAATCTCGTGCCTCCCAATTGGGGAGCCGATATCCATTCCTGGCTCCTTGCCTTCGCTTCCAAATCCCCAATTCTCGAAGAATTGAGGCTCAAGAGGATGGCCGTCACAGACGAGAGCTTGGAATTTCTGGCTCGTTCCTTCCCAAATTTCAAATCCATTTCACTCATGAGCTGCGATGGCTTCAGCACCGATGGGCTTGCCGCCATTGCCACACACTGCAAGAACTTGACGGAGCTTGACATACTTGAGAATGACATTATTGACAAAAGTGGAAATTGGCTGAGTTGCTTCCCGGATACATTGAAATCTCTGGAAGTACTAAATTTTGCCAGTTTGAACAGCGATGTCAGTTTCGAGGCCCTCGAGAAGCTGGTAAGAAGGTGCAAATCATTGAAGGTTTTGAAGGTTAATAGGAACATAAACTTGGAACAGTTGCAGAGGCTGCTTGTTCACGTTCCGCAGTTAACTGAGTTAGGCACGGGCTCTTTTTTGCAAAAGATCACTCTTCGGCAGTACTGTGACCTCGAAGATGCGTTTAAGAACTGCAAGAATCTGCATACCCTCTCTGGTTTGTTGGAGTCGACGGTGTTGTATCTCCAAGTTCTGTTTCCTGCCTGCACGAATTTAACTTTCTTGAATCTGAGCTACGCAACTCTGCATGGTGGCGAACTTGCAGGACTTCTTTCCCACTGTCCCGTTCTTCGTCGCCTTTGGGTTTTAGACACAGTTGAAGACAAGGGCCTGAAGGCTGTTGGAATAAGTTGCCCCTTGCTAGAGGAACTCCGTGTATTTCCAGCGGATCCGTATGCTGACAATCTAGTTCACGGTGTAACCGAGTCTGGGTTTCTTGCTGTCTCTTATGGCTGCCGTAAACTCCATTACGTTCTCTACTTCTGTCGCCAGATGACGAATGAGGCTGTAGCCACCATTGTGCAGAACTGCCCAGATTTCACCCATTTTCGTCTCTGCATCATGAACCCTGCCCAACCAGACTATCTCACAAATCAATCAATGGACGAGGCCTTTGGTGCTGTGGTGAAAACTTGCTCGAAACTACGTAGACTTGCCATTTCAGGCTTGCTAACGGACTTAGCATTTGAATACATCGGTAAATATGCGAAAAACTTGGAAACTCTTTCAGTGGCTTTTGCTGGAAAGAGCGACTATGGAATGCAGTGTGTTATGAATGGTTGTCCAAAGCTGAGGAAACTGGAAATTAGAGACAGCCCCTTTGGAAATGCTGCATTGCTTTCGGGTATGGAGAGGTACGAATCCATGAGATCGCTATGGATGTCTGCCTGCAAGGTGACAAGGAATGGGTGCCGAATTCTGGCAAAGCAGGTGCCTAGGTTGAATGTCGAGGTCATCAAGGGTGACAACTGCGGGAGTGATGAGGCTGAAAGCGTTTACGTATATCGCTCTGTCGCTGGTCCAAGAAAAGACGCCCCATCCTGCGTCCTCACCCTC

Coding sequence (CDS)

AATTCGAAGAGGAAGAAGAAAGAGTCGGGTGACTCGGACGAGTCGAGTTTCCCAGATGAAGTTTTGGAACGAGTTCTGAGCTTGGTTAAGTCCCACAAAGACAGAAGCTCTGTTTCTCTGGTTTGTAAGGAATGGTACAACGCCGAGCGCTGGTCCCGGACCCACGTCTTCATCGGAAACTGCTACTCTGTCTCGCCGGAAATTGTAATCCGCCGGTTCCCCAACATCCGGAGCGTCACCATTAAAGGGAAGCCCCGGTTCTCGGATTTCAATCTCGTGCCTCCCAATTGGGGAGCCGATATCCATTCCTGGCTCCTTGCCTTCGCTTCCAAATCCCCAATTCTCGAAGAATTGAGGCTCAAGAGGATGGCCGTCACAGACGAGAGCTTGGAATTTCTGGCTCGTTCCTTCCCAAATTTCAAATCCATTTCACTCATGAGCTGCGATGGCTTCAGCACCGATGGGCTTGCCGCCATTGCCACACACTGCAAGAACTTGACGGAGCTTGACATACTTGAGAATGACATTATTGACAAAAGTGGAAATTGGCTGAGTTGCTTCCCGGATACATTGAAATCTCTGGAAGTACTAAATTTTGCCAGTTTGAACAGCGATGTCAGTTTCGAGGCCCTCGAGAAGCTGGTAAGAAGGTGCAAATCATTGAAGGTTTTGAAGGTTAATAGGAACATAAACTTGGAACAGTTGCAGAGGCTGCTTGTTCACGTTCCGCAGTTAACTGAGTTAGGCACGGGCTCTTTTTTGCAAAAGATCACTCTTCGGCAGTACTGTGACCTCGAAGATGCGTTTAAGAACTGCAAGAATCTGCATACCCTCTCTGGTTTGTTGGAGTCGACGGTGTTGTATCTCCAAGTTCTGTTTCCTGCCTGCACGAATTTAACTTTCTTGAATCTGAGCTACGCAACTCTGCATGGTGGCGAACTTGCAGGACTTCTTTCCCACTGTCCCGTTCTTCGTCGCCTTTGGGTTTTAGACACAGTTGAAGACAAGGGCCTGAAGGCTGTTGGAATAAGTTGCCCCTTGCTAGAGGAACTCCGTGTATTTCCAGCGGATCCGTATGCTGACAATCTAGTTCACGGTGTAACCGAGTCTGGGTTTCTTGCTGTCTCTTATGGCTGCCGTAAACTCCATTACGTTCTCTACTTCTGTCGCCAGATGACGAATGAGGCTGTAGCCACCATTGTGCAGAACTGCCCAGATTTCACCCATTTTCGTCTCTGCATCATGAACCCTGCCCAACCAGACTATCTCACAAATCAATCAATGGACGAGGCCTTTGGTGCTGTGGTGAAAACTTGCTCGAAACTACGTAGACTTGCCATTTCAGGCTTGCTAACGGACTTAGCATTTGAATACATCGGTAAATATGCGAAAAACTTGGAAACTCTTTCAGTGGCTTTTGCTGGAAAGAGCGACTATGGAATGCAGTGTGTTATGAATGGTTGTCCAAAGCTGAGGAAACTGGAAATTAGAGACAGCCCCTTTGGAAATGCTGCATTGCTTTCGGGTATGGAGAGGTACGAATCCATGAGATCGCTATGGATGTCTGCCTGCAAGGTGACAAGGAATGGGTGCCGAATTCTGGCAAAGCAGGTGCCTAGGTTGAATGTCGAGGTCATCAAGGGTGACAACTGCGGGAGTGATGAGGCTGAAAGCGTTTACGTATATCGCTCTGTCGCTGGTCCAAGAAAAGACGCCCCATCCTGCGTCCTCACCCTC

Protein sequence

NSKRKKKESGDSDESSFPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGNCYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRLKRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQPDYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYGMQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPRLNVEVIKGDNCGSDEAESVYVYRSVAGPRKDAPSCVLTL
Homology
BLAST of MS010820 vs. NCBI nr
Match: XP_022142182.1 (protein TRANSPORT INHIBITOR RESPONSE 1-like [Momordica charantia] >XP_022142183.1 protein TRANSPORT INHIBITOR RESPONSE 1-like [Momordica charantia])

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 577/579 (99.65%), Postives = 579/579 (100.00%), Query Frame = 0

Query: 1   NSKRKKKESGDSDESSFPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGN 60
           NSKRKKKESGDSDESSFPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGN
Sbjct: 3   NSKRKKKESGDSDESSFPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGN 62

Query: 61  CYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRL 120
           CYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRL
Sbjct: 63  CYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRL 122

Query: 121 KRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKS 180
           KRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKS
Sbjct: 123 KRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKS 182

Query: 181 GNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLV 240
           GNWLSCFPDTLKSLEVLNF+SLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLV
Sbjct: 183 GNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLV 242

Query: 241 HVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLT 300
           HVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLT
Sbjct: 243 HVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLT 302

Query: 301 FLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYA 360
           FLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYA
Sbjct: 303 FLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYA 362

Query: 361 DNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQP 420
           DNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQP
Sbjct: 363 DNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQP 422

Query: 421 DYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYG 480
           DYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYG
Sbjct: 423 DYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYG 482

Query: 481 MQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPR 540
           MQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPR
Sbjct: 483 MQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPR 542

Query: 541 LNVEVIKGDNCGSDEAESVYVYRSVAGPRKDAPSCVLTL 580
           LNVEVIKGD+CGSDEAESVYVYRSVAGPRKDAPSCVLTL
Sbjct: 543 LNVEVIKGDSCGSDEAESVYVYRSVAGPRKDAPSCVLTL 581

BLAST of MS010820 vs. NCBI nr
Match: XP_038883074.1 (protein TRANSPORT INHIBITOR RESPONSE 1-like [Benincasa hispida])

HSP 1 Score: 1074.7 bits (2778), Expect = 4.4e-310
Identity = 527/583 (90.39%), Postives = 553/583 (94.85%), Query Frame = 0

Query: 2   SKRKKKESGDSDESS-----FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHV 61
           S +++K SGDSDES+     FPDEVLERVLSLVKSHKDRSSVSLVCK+W+NAERWSRTHV
Sbjct: 2   SSKRQKGSGDSDESNRVGSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHV 61

Query: 62  FIGNCYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILE 121
           FIGNCYSVSPEIVIRRFPNIRSVT+KGKPRFSDFNLVPPNWGADIHSWL+AFASK P LE
Sbjct: 62  FIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPNLE 121

Query: 122 ELRLKRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDI 181
           ELRLKRM VTDESLEFL+RSFPNFK++S+MSCDGFSTDGLAAIATHCKNLTELDILENDI
Sbjct: 122 ELRLKRMTVTDESLEFLSRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILENDI 181

Query: 182 IDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQ 241
            DKSG+WLSCFPDTLKSLEVLNF+SLNSD+SFEALEKLVRRCKSLKVLKVNRNINLEQLQ
Sbjct: 182 NDKSGHWLSCFPDTLKSLEVLNFSSLNSDISFEALEKLVRRCKSLKVLKVNRNINLEQLQ 241

Query: 242 RLLVHVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPAC 301
           RLLVH PQLTELGTGSF Q+ITLRQYCDLEDAFK+CKNL TLSGLLESTVLYLQVLFPAC
Sbjct: 242 RLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPAC 301

Query: 302 TNLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPA 361
            NLTFLNLSY+ LHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVG+SCPLLEELRVFPA
Sbjct: 302 ANLTFLNLSYSILHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFPA 361

Query: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421
           DP+ADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN
Sbjct: 362 DPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421

Query: 422 PAQPDYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGK 481
           P QPDYLT QSMDEAFGAVVKTCSKLRRLAISGLLTDL FEYIGKYAKNLETLSVAFAG 
Sbjct: 422 PHQPDYLTEQSMDEAFGAVVKTCSKLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGS 481

Query: 482 SDYGMQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAK 541
           SD+GMQCVM+GCPKLRKLEIRDSPFGNAALLSG+ERYESMRSLWMSACKVT NGCR+LAK
Sbjct: 482 SDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAK 541

Query: 542 QVPRLNVEVIKGDNCGSDEAESVYVYRSVAGPRKDAPSCVLTL 580
           QVPRLN+EVIK       EAESVYVYRSVAGPR+DAP  VLTL
Sbjct: 542 QVPRLNIEVIKDGGSDECEAESVYVYRSVAGPRRDAPPFVLTL 584

BLAST of MS010820 vs. NCBI nr
Match: XP_023002910.1 (protein AUXIN SIGNALING F-BOX 2-like [Cucurbita maxima])

HSP 1 Score: 1074.3 bits (2777), Expect = 4.4e-310
Identity = 532/586 (90.78%), Postives = 553/586 (94.37%), Query Frame = 0

Query: 2   SKRKKKESGDSDESS-----FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHV 61
           S ++KKE GD DES      FPDEVLERVLSLVKSHKDRSSVSLVCK+W+NAERWSRTHV
Sbjct: 2   SSKRKKELGDLDESKRAGSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHV 61

Query: 62  FIGNCYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILE 121
           FIGNCYSVSPEIVIRRFPNIRSVT+KGKPRFSDFNLVPPNWGADIHSWL++FAS+ PILE
Sbjct: 62  FIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVSFASRYPILE 121

Query: 122 ELRLKRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDI 181
           ELRLKRM VTDESLEFLARSFPNFK++SLMSCDGFSTDGLAAIATHCKNLT+LDILENDI
Sbjct: 122 ELRLKRMTVTDESLEFLARSFPNFKALSLMSCDGFSTDGLAAIATHCKNLTDLDILENDI 181

Query: 182 IDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQ 241
            DKSG+WLSCFPDTLKSLEVLNFASLNSDVSFEALEKLV+RCKSLKVLKVNRNINLEQLQ
Sbjct: 182 YDKSGSWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVKRCKSLKVLKVNRNINLEQLQ 241

Query: 242 RLLVHVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPAC 301
           RLLVH PQLTELGTGSF Q+ITLRQY DLE AFKNC NLHTLSGLLESTVLYLQVLFPAC
Sbjct: 242 RLLVHAPQLTELGTGSFSQEITLRQYYDLEHAFKNCNNLHTLSGLLESTVLYLQVLFPAC 301

Query: 302 TNLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPA 361
            NLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVG+SCPLLEELRVFPA
Sbjct: 302 ANLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFPA 361

Query: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421
           DPYADNLVHGVTESGFLAVSYGCRKLHY+LYFCRQMTNEAVATIVQNCPDFTHFRLCIMN
Sbjct: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYILYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421

Query: 422 PAQPDYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGK 481
           P QPDYLT Q MDEAFGAVVKTCSKLRRLAISGLLTDL FEYIGKYAKNLETLSVAFAG 
Sbjct: 422 PCQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGT 481

Query: 482 SDYGMQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAK 541
           SD+GMQCVM+GCPKLRKLEIRDSPFGNAALLSG+ERYESMRSLWMSACKVT NGCR+LAK
Sbjct: 482 SDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAK 541

Query: 542 QVPRLNVEVIK---GDNCGSDEAESVYVYRSVAGPRKDAPSCVLTL 580
           QVPRLNVEVIK   GD C   EAESVYVYRSVAG R+DAPS VLTL
Sbjct: 542 QVPRLNVEVIKAGGGDEC---EAESVYVYRSVAGRRRDAPSFVLTL 584

BLAST of MS010820 vs. NCBI nr
Match: XP_023518017.1 (protein AUXIN SIGNALING F-BOX 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1073.9 bits (2776), Expect = 4.4e-310
Identity = 529/583 (90.74%), Postives = 549/583 (94.17%), Query Frame = 0

Query: 2   SKRKKKESGDSDESS-----FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHV 61
           S ++KKE GD DES      FPDEVLERVLSLVKSHKDRSSVSLVCK+W+NAERWSRTHV
Sbjct: 2   SSKRKKELGDLDESKRAGSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHV 61

Query: 62  FIGNCYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILE 121
           FIGNCYSVSPEIVIRRFPNIRSVT+KGKPRFSDFNLVPPNWGAD+HSWLL+FASK PILE
Sbjct: 62  FIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADVHSWLLSFASKYPILE 121

Query: 122 ELRLKRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDI 181
           ELRLKRM VTDESLEFLARSFPNFK++SLMSCDGFSTDGLAAIATHCKNLT+LDILENDI
Sbjct: 122 ELRLKRMTVTDESLEFLARSFPNFKALSLMSCDGFSTDGLAAIATHCKNLTDLDILENDI 181

Query: 182 IDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQ 241
            DKSG+WLSCFPDTLKS+EVLNFASLNSDVSFEALEKLV+RCKSLKVLKVNRNINLEQLQ
Sbjct: 182 YDKSGSWLSCFPDTLKSMEVLNFASLNSDVSFEALEKLVKRCKSLKVLKVNRNINLEQLQ 241

Query: 242 RLLVHVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPAC 301
           RLLVH PQLTELGTGSF Q+ITLRQY DLE AFKNC NLHTLSGLLESTVLYLQVLFPAC
Sbjct: 242 RLLVHAPQLTELGTGSFSQEITLRQYYDLEHAFKNCNNLHTLSGLLESTVLYLQVLFPAC 301

Query: 302 TNLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPA 361
            NLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVG+SCPLLEELRVFPA
Sbjct: 302 ANLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFPA 361

Query: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421
           DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN
Sbjct: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421

Query: 422 PAQPDYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGK 481
           P QPDYLT Q MDEAFGAVVKTCSKLRRLAISGLLTDL FEYIGKYA NLETLSVAFAG 
Sbjct: 422 PCQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDLTFEYIGKYATNLETLSVAFAGT 481

Query: 482 SDYGMQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAK 541
           SD+GMQCVM+GCPKLRKLEIRDSPFGNAALLSG+ERYESMRSLWMSACKVT NGCR+LAK
Sbjct: 482 SDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAK 541

Query: 542 QVPRLNVEVIKGDNCGSDEAESVYVYRSVAGPRKDAPSCVLTL 580
           QVPRLNVEVIK       EAESVYVYRSVAG R+DAPS VLTL
Sbjct: 542 QVPRLNVEVIKAGGSDECEAESVYVYRSVAGRRRDAPSFVLTL 584

BLAST of MS010820 vs. NCBI nr
Match: XP_022926770.1 (protein AUXIN SIGNALING F-BOX 2-like [Cucurbita moschata])

HSP 1 Score: 1073.2 bits (2774), Expect = 8.9e-310
Identity = 528/583 (90.57%), Postives = 549/583 (94.17%), Query Frame = 0

Query: 2   SKRKKKESGDSDESS-----FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHV 61
           S ++KKE GD DES      FPDEVLERVLSLVKSHKDRSSVSLVCK+W+NAERWSRTHV
Sbjct: 2   SSKRKKELGDLDESKRAGSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHV 61

Query: 62  FIGNCYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILE 121
           FIGNCYSVSPEIV+RRFPNIRSVT+KGKPRFSDFNLVPPNWGAD+HSWL++FASK PILE
Sbjct: 62  FIGNCYSVSPEIVVRRFPNIRSVTLKGKPRFSDFNLVPPNWGADVHSWLVSFASKYPILE 121

Query: 122 ELRLKRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDI 181
           ELRLKRM VTDESLEFLARSFPNFK++SLMSCDGFSTDGLAAIATHCKNLT+LDILENDI
Sbjct: 122 ELRLKRMTVTDESLEFLARSFPNFKALSLMSCDGFSTDGLAAIATHCKNLTDLDILENDI 181

Query: 182 IDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQ 241
            DKSG+WLSCFPDTLKSLEVLNFASLNSDVSFEALEKLV+RCKSLKVLKVNRNINLEQLQ
Sbjct: 182 YDKSGSWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVKRCKSLKVLKVNRNINLEQLQ 241

Query: 242 RLLVHVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPAC 301
           RLLVH PQLTELGTGSF Q+ITLRQY DLE AFKNC NLHTLSGLLESTVLYLQVLFPAC
Sbjct: 242 RLLVHAPQLTELGTGSFSQEITLRQYYDLEHAFKNCNNLHTLSGLLESTVLYLQVLFPAC 301

Query: 302 TNLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPA 361
            NLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVG+SCPLLEELRVFPA
Sbjct: 302 ANLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFPA 361

Query: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421
           DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN
Sbjct: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421

Query: 422 PAQPDYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGK 481
           P QPDYLT Q MDEAFGAVVKTCSKLRRLAISGLLTD  FEYIGKYAKNLETLSVAFAG 
Sbjct: 422 PCQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDSTFEYIGKYAKNLETLSVAFAGT 481

Query: 482 SDYGMQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAK 541
           SD+GMQCVM+GCPKLRKLEIRDSPFGNAALLSG+ERYESMRSLWMSACKVT NGCR+LAK
Sbjct: 482 SDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAK 541

Query: 542 QVPRLNVEVIKGDNCGSDEAESVYVYRSVAGPRKDAPSCVLTL 580
           QVPRLNVEVIK       EAESVYVYRSVAG R+DAPS VLTL
Sbjct: 542 QVPRLNVEVIKAGGSDECEAESVYVYRSVAGRRRDAPSFVLTL 584

BLAST of MS010820 vs. ExPASy Swiss-Prot
Match: Q9LW29 (Protein AUXIN SIGNALING F-BOX 2 OS=Arabidopsis thaliana OX=3702 GN=AFB2 PE=1 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 6.2e-180
Identity = 308/573 (53.75%), Postives = 402/573 (70.16%), Query Frame = 0

Query: 17  FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 76
           FPDEV+E V   V SHKDR+++SLVCK WY  ER+SR  VFIGNCY+++PE ++RRFP +
Sbjct: 4   FPDEVIEHVFDFVTSHKDRNAISLVCKSWYKIERYSRQKVFIGNCYAINPERLLRRFPCL 63

Query: 77  RSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRLKRMAVTDESLEFLARS 136
           +S+T+KGKP F+DFNLVP  WG  +  W+ A A     LEELRLKRM VTDESLE L+RS
Sbjct: 64  KSLTLKGKPHFADFNLVPHEWGGFVLPWIEALARSRVGLEELRLKRMVVTDESLELLSRS 123

Query: 137 FPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 196
           F NFKS+ L+SC+GF+TDGLA+IA +C++L +LD+ EN+I D  G WLSCFPDT  +L  
Sbjct: 124 FVNFKSLVLVSCEGFTTDGLASIAANCRHLRDLDLQENEIDDHRGQWLSCFPDTCTTLVT 183

Query: 197 LNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHVPQLTELGTGSFLQK 256
           LNFA L  + +  ALE+LV R  +LK LK+NR + L+ L RL+   PQ+ +LG GS+   
Sbjct: 184 LNFACLEGETNLVALERLVARSPNLKSLKLNRAVPLDALARLMACAPQIVDLGVGSYEND 243

Query: 257 ITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLTFLNLSYAT-LHGGELA 316
                Y  L    K C +L +LSG LE+    L    P C NLT LNLSYA  +HG  L 
Sbjct: 244 PDSESYLKLMAVIKKCTSLRSLSGFLEAAPHCLSAFHPICHNLTSLNLSYAAEIHGSHLI 303

Query: 317 GLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYADNLVHGVTESGFLAV 376
            L+ HC  L+RLW+LD++ DKGL+ V  +C  L+ELRVFP+D         VTE G +A+
Sbjct: 304 KLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQELRVFPSDLLGGGNT-AVTEEGLVAI 363

Query: 377 SYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQPDYLTNQSMDEAFGAV 436
           S GC KLH +LYFC+QMTN A+ T+ +NCP+F  FRLCI+ P +PD++T+Q +DE FGA+
Sbjct: 364 SAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRLCILEPNKPDHVTSQPLDEGFGAI 423

Query: 437 VKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYGMQCVMNGCPKLRKLE 496
           VK C  LRRL++SGLLTD  F YIG YA  LE LS+AFAG +D GM  V+NGC K++KLE
Sbjct: 424 VKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSIAFAGDTDKGMLYVLNGCKKMKKLE 483

Query: 497 IRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPRLNVEVI-KGDNC--- 556
           IRDSPFG+ ALL+ + +YE+MRSLWMS+C+VT +GC+ LA++ P LNVE+I + DN    
Sbjct: 484 IRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSGCKRLAEKAPWLNVEIINENDNNRME 543

Query: 557 -----GSDEAESVYVYRSVAGPRKDAPSCVLTL 580
                G  + + +Y+YR+V G R DAP  V  L
Sbjct: 544 ENGHEGRQKVDKLYLYRTVVGTRMDAPPFVWIL 575

BLAST of MS010820 vs. ExPASy Swiss-Prot
Match: Q570C0 (Protein TRANSPORT INHIBITOR RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=TIR1 PE=1 SV=2)

HSP 1 Score: 632.1 bits (1629), Expect = 6.2e-180
Identity = 314/568 (55.28%), Postives = 396/568 (69.72%), Query Frame = 0

Query: 16  SFPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGNCYSVSPEIVIRRFPN 75
           SFP+EVLE V S ++  KDR+SVSLVCK WY  ERW R  VFIGNCY+VSP  VIRRFP 
Sbjct: 8   SFPEEVLEHVFSFIQLDKDRNSVSLVCKSWYEIERWCRRKVFIGNCYAVSPATVIRRFPK 67

Query: 76  IRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRLKRMAVTDESLEFLAR 135
           +RSV +KGKP F+DFNLVP  WG  ++ W+ A +S    LEE+RLKRM VTD+ LE +A+
Sbjct: 68  VRSVELKGKPHFADFNLVPDGWGGYVYPWIEAMSSSYTWLEEIRLKRMVVTDDCLELIAK 127

Query: 136 SFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLE 195
           SF NFK + L SC+GFSTDGLAAIA  C+NL ELD+ E+D+ D SG+WLS FPDT  SL 
Sbjct: 128 SFKNFKVLVLSSCEGFSTDGLAAIAATCRNLKELDLRESDVDDVSGHWLSHFPDTYTSLV 187

Query: 196 VLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHVPQLTELGTGSFLQ 255
            LN + L S+VSF ALE+LV RC +LK LK+NR + LE+L  LL   PQL ELGTG +  
Sbjct: 188 SLNISCLASEVSFSALERLVTRCPNLKSLKLNRAVPLEKLATLLQRAPQLEELGTGGYTA 247

Query: 256 KITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLTFLNLSYATLHGGELA 315
           ++    Y  L  A   CK L  LSG  ++   YL  ++  C+ LT LNLSYAT+   +L 
Sbjct: 248 EVRPDVYSGLSVALSGCKELRCLSGFWDAVPAYLPAVYSVCSRLTTLNLSYATVQSYDLV 307

Query: 316 GLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYADNLVHGVTESGFLAV 375
            LL  CP L+RLWVLD +ED GL+ +  +C  L ELRVFP++P+       +TE G ++V
Sbjct: 308 KLLCQCPKLQRLWVLDYIEDAGLEVLASTCKDLRELRVFPSEPFVMEPNVALTEQGLVSV 367

Query: 376 SYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQPDYLTNQSMDEAFGAV 435
           S GC KL  VLYFCRQMTN A+ TI +N P+ T FRLCI+ P  PDYLT + +D  FGA+
Sbjct: 368 SMGCPKLESVLYFCRQMTNAALITIARNRPNMTRFRLCIIEPKAPDYLTLEPLDIGFGAI 427

Query: 436 VKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYGMQCVMNGCPKLRKLE 495
           V+ C  LRRL++SGLLTD  FEYIG YAK +E LSVAFAG SD GM  V++GC  LRKLE
Sbjct: 428 VEHCKDLRRLSLSGLLTDKVFEYIGTYAKKMEMLSVAFAGDSDLGMHHVLSGCDSLRKLE 487

Query: 496 IRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPRLNVEVIKGDNCGSDE 555
           IRD PFG+ ALL+   + E+MRSLWMS+C V+   C++L +++P+LNVEVI  D  G+ +
Sbjct: 488 IRDCPFGDKALLANASKLETMRSLWMSSCSVSFGACKLLGQKMPKLNVEVI--DERGAPD 547

Query: 556 A-------ESVYVYRSVAGPRKDAPSCV 577
           +       E V++YR+VAGPR D P  V
Sbjct: 548 SRPESCPVERVFIYRTVAGPRFDMPGFV 573

BLAST of MS010820 vs. ExPASy Swiss-Prot
Match: Q9LPW7 (Protein AUXIN SIGNALING F-BOX 3 OS=Arabidopsis thaliana OX=3702 GN=AFB3 PE=1 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 2.4e-179
Identity = 316/575 (54.96%), Postives = 406/575 (70.61%), Query Frame = 0

Query: 17  FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 76
           FPDEV+E V   V SHKDR+S+SLVCK W+  ER+SR  VFIGNCY+++PE +IRRFP +
Sbjct: 4   FPDEVIEHVFDFVASHKDRNSISLVCKSWHKIERFSRKEVFIGNCYAINPERLIRRFPCL 63

Query: 77  RSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRLKRMAVTDESLEFLARS 136
           +S+T+KGKP F+DFNLVP  WG  +H W+ A A     LEELRLKRM VTDESL+ L+RS
Sbjct: 64  KSLTLKGKPHFADFNLVPHEWGGFVHPWIEALARSRVGLEELRLKRMVVTDESLDLLSRS 123

Query: 137 FPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 196
           F NFKS+ L+SC+GF+TDGLA+IA +C++L ELD+ EN+I D  G WL+CFPD+  +L  
Sbjct: 124 FANFKSLVLVSCEGFTTDGLASIAANCRHLRELDLQENEIDDHRGQWLNCFPDSCTTLMS 183

Query: 197 LNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHVPQLTELGTGSFLQK 256
           LNFA L  + +  ALE+LV R  +LK LK+NR + L+ L RL+   PQL +LG GS+  +
Sbjct: 184 LNFACLKGETNVAALERLVARSPNLKSLKLNRAVPLDALARLMSCAPQLVDLGVGSYENE 243

Query: 257 ITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLTFLNLSYAT-LHGGELA 316
                +  L  A K   +L +LSG LE   L L   +P C NL  LNLSYA  + G  L 
Sbjct: 244 PDPESFAKLMTAIKKYTSLRSLSGFLEVAPLCLPAFYPICQNLISLNLSYAAEIQGNHLI 303

Query: 317 GLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYA--DNLVHGVTESGFL 376
            L+  C  L+RLW+LD++ DKGL  V  +C  L+ELRVFP+D +   DN    VTE G +
Sbjct: 304 KLIQLCKRLQRLWILDSIGDKGLAVVAATCKELQELRVFPSDVHGEEDNNA-SVTEVGLV 363

Query: 377 AVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQPDYLTNQSMDEAFG 436
           A+S GC KLH +LYFC+QMTN A+  + +NCP+F  FRLCI+ P +PD++T QS+DE FG
Sbjct: 364 AISAGCPKLHSILYFCKQMTNAALIAVAKNCPNFIRFRLCILEPHKPDHITFQSLDEGFG 423

Query: 437 AVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYGMQCVMNGCPKLRK 496
           A+V+ C  LRRL++SGLLTD  F YIG YA+ LE LS+AFAG +D GM  V+NGC K+RK
Sbjct: 424 AIVQACKGLRRLSVSGLLTDQVFLYIGMYAEQLEMLSIAFAGDTDKGMLYVLNGCKKMRK 483

Query: 497 LEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPRLNVEVI-KGDNCG 556
           LEIRDSPFGNAALL+ + RYE+MRSLWMS+C+VT  GC+ LA+  PRLNVE+I + +N G
Sbjct: 484 LEIRDSPFGNAALLADVGRYETMRSLWMSSCEVTLGGCKRLAQNSPRLNVEIINENENNG 543

Query: 557 -----SDEAESV---YVYRSVAGPRKDAPSCVLTL 580
                 DE E V   Y+YR+V G RKDAP  V  L
Sbjct: 544 MEQNEEDEREKVDKLYLYRTVVGTRKDAPPYVRIL 577

BLAST of MS010820 vs. ExPASy Swiss-Prot
Match: Q7XVM8 (Transport inhibitor response 1-like protein Os04g0395600 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0395600 PE=2 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 2.6e-178
Identity = 308/574 (53.66%), Postives = 403/574 (70.21%), Query Frame = 0

Query: 17  FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 76
           FP+EV+E + S + + +DR++VSLVCK WY  ER SR  VF+GNCY+V    V  RFPN+
Sbjct: 4   FPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVGNCYAVRAGRVAARFPNV 63

Query: 77  RSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRLKRMAVTDESLEFLARS 136
           R++T+KGKP F+DFNLVPP+WG     W+ A A     LEELR+KRM V+DESLE LARS
Sbjct: 64  RALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEELRMKRMVVSDESLELLARS 123

Query: 137 FPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 196
           FP F+++ L+SC+GFSTDGLAA+A+HCK L ELD+ EN++ D+   WLSCFPD+  SL  
Sbjct: 124 FPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVEDRGPRWLSCFPDSCTSLVS 183

Query: 197 LNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHVPQLTELGTGSFLQK 256
           LNFA +  +V+  +LE+LV R  +L+ L++NR+++++ L ++L+  P L +LGTG+    
Sbjct: 184 LNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLAKILLRTPNLEDLGTGNLTDD 243

Query: 257 ITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLTFLNLSYA-TLHGGELA 316
                Y  L  A + CK L +LSG  +++ + L  ++P C  LT LNLSYA TL   +L 
Sbjct: 244 FQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPLCAQLTGLNLSYAPTLDASDLT 303

Query: 317 GLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYADNLVHGVTESGFLAV 376
            ++S C  L+RLWVLD + DKGL+ V  SC  L+ELRVFP+D Y       VTE G +AV
Sbjct: 304 KMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRVFPSDFYVAG-YSAVTEEGLVAV 363

Query: 377 SYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQPDYLTNQSMDEAFGAV 436
           S GC KL+ +LYFC QMTN A+ T+ +NCP+FT FRLCI+ P +PD +T+Q +DE FGA+
Sbjct: 364 SLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLCILEPGKPDVVTSQPLDEGFGAI 423

Query: 437 VKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYGMQCVMNGCPKLRKLE 496
           V+ C  L+RL+ISGLLTD  F YIGKYAK LE LS+AFAG SD GM  VMNGC  LRKLE
Sbjct: 424 VRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIAFAGDSDKGMMHVMNGCKNLRKLE 483

Query: 497 IRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPRLNVEVIKGDNCGSDE 556
           IRDSPFG+AALL    RYE+MRSLWMS+C VT  GC++LA ++P LNVEVI  +  GS+E
Sbjct: 484 IRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGCQVLASKMPMLNVEVI-NERDGSNE 543

Query: 557 ----------AESVYVYRSVAGPRKDAPSCVLTL 580
                      E +YVYR+ AG R DAP+ V  L
Sbjct: 544 MEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL 575

BLAST of MS010820 vs. ExPASy Swiss-Prot
Match: Q9ZR12 (GRR1-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=GRH1 PE=1 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 3.6e-164
Identity = 290/567 (51.15%), Postives = 385/567 (67.90%), Query Frame = 0

Query: 17  FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 76
           FP +VLE +LS + S++DR+SVSLVCK W+  ER +R  VF+GNCY+VSP  V RRFP +
Sbjct: 5   FPPKVLEHILSFIDSNEDRNSVSLVCKSWFETERKTRKRVFVGNCYAVSPAAVTRRFPEM 64

Query: 77  RSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRLKRMAVTDESLEFLARS 136
           RS+T+KGKP F+D+NLVP  WG     W+ A A+KS  LEE+R+KRM VTDE LE +A S
Sbjct: 65  RSLTLKGKPHFADYNLVPDGWGGYAWPWIEAMAAKSSSLEEIRMKRMVVTDECLEKIAAS 124

Query: 137 FPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 196
           F +FK + L SC+GFSTDG+AAIA  C+NL  L++ E  + D  G+WLS FP++  SL  
Sbjct: 125 FKDFKVLVLTSCEGFSTDGIAAIAATCRNLRVLELRECIVEDLGGDWLSYFPESSTSLVS 184

Query: 197 LNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHVPQLTELGTGSFLQK 256
           L+F+ L+S+V    LE+LV R  +LK LK+N  + L+ L  LL   PQLTELGTGSF  +
Sbjct: 185 LDFSCLDSEVKISDLERLVSRSPNLKSLKLNPAVTLDGLVSLLRCAPQLTELGTGSFAAQ 244

Query: 257 ITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLTFLNLSYATLHGGELAG 316
           +    +  L +AF NCK L +LSGL +    YL  L+  C  LT LNLSYAT+   +L  
Sbjct: 245 LKPEAFSKLSEAFSNCKQLQSLSGLWDVLPEYLPALYSVCPGLTSLNLSYATVRMPDLVE 304

Query: 317 LLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYADNLVHGVTESGFLAVS 376
           LL  C  L++LWV+D +EDKGL+AV   C  L ELRVFP++P  D     +TE G + VS
Sbjct: 305 LLRRCSKLQKLWVMDLIEDKGLEAVASYCKELRELRVFPSEPDLDATNIPLTEQGLVFVS 364

Query: 377 YGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQPDYLTNQSMDEAFGAVV 436
            GCRKL  VLYFC Q TN A+ TI +  P+   FRLC++ P  PDY TN+ +D+ F A+ 
Sbjct: 365 KGCRKLESVLYFCVQFTNAALFTIARKRPNLKCFRLCVIEPFAPDYKTNEPLDKGFKAIA 424

Query: 437 KTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYGMQCVMNGCPKLRKLEI 496
           + C  LRRL++SGLL+D AF+YIGK+AK +  LS+AFAG SD  +  +++GC  L+KLEI
Sbjct: 425 EGCRDLRRLSVSGLLSDKAFKYIGKHAKKVRMLSIAFAGDSDLMLHHLLSGCESLKKLEI 484

Query: 497 RDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPRLNVEVI----KGDNCG 556
           RD PFG+ ALL    + E+MRSLWMS+C V+   C++L++++PRLNVEVI          
Sbjct: 485 RDCPFGDTALLEHAAKLETMRSLWMSSCFVSFGACKLLSQKMPRLNVEVIDEHPPESRPE 544

Query: 557 SDEAESVYVYRSVAGPRKDAPSCVLTL 580
           S   E +Y+YR+VAGPR D P  V T+
Sbjct: 545 SSPVERIYIYRTVAGPRMDTPEFVWTI 571

BLAST of MS010820 vs. ExPASy TrEMBL
Match: A0A6J1CM08 (protein TRANSPORT INHIBITOR RESPONSE 1-like OS=Momordica charantia OX=3673 GN=LOC111012360 PE=4 SV=1)

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 577/579 (99.65%), Postives = 579/579 (100.00%), Query Frame = 0

Query: 1   NSKRKKKESGDSDESSFPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGN 60
           NSKRKKKESGDSDESSFPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGN
Sbjct: 3   NSKRKKKESGDSDESSFPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGN 62

Query: 61  CYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRL 120
           CYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRL
Sbjct: 63  CYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRL 122

Query: 121 KRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKS 180
           KRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKS
Sbjct: 123 KRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKS 182

Query: 181 GNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLV 240
           GNWLSCFPDTLKSLEVLNF+SLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLV
Sbjct: 183 GNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLV 242

Query: 241 HVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLT 300
           HVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLT
Sbjct: 243 HVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLT 302

Query: 301 FLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYA 360
           FLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYA
Sbjct: 303 FLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYA 362

Query: 361 DNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQP 420
           DNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQP
Sbjct: 363 DNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQP 422

Query: 421 DYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYG 480
           DYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYG
Sbjct: 423 DYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYG 482

Query: 481 MQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPR 540
           MQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPR
Sbjct: 483 MQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPR 542

Query: 541 LNVEVIKGDNCGSDEAESVYVYRSVAGPRKDAPSCVLTL 580
           LNVEVIKGD+CGSDEAESVYVYRSVAGPRKDAPSCVLTL
Sbjct: 543 LNVEVIKGDSCGSDEAESVYVYRSVAGPRKDAPSCVLTL 581

BLAST of MS010820 vs. ExPASy TrEMBL
Match: A0A6J1KQA4 (protein AUXIN SIGNALING F-BOX 2-like OS=Cucurbita maxima OX=3661 GN=LOC111496678 PE=4 SV=1)

HSP 1 Score: 1074.3 bits (2777), Expect = 2.2e-310
Identity = 532/586 (90.78%), Postives = 553/586 (94.37%), Query Frame = 0

Query: 2   SKRKKKESGDSDESS-----FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHV 61
           S ++KKE GD DES      FPDEVLERVLSLVKSHKDRSSVSLVCK+W+NAERWSRTHV
Sbjct: 2   SSKRKKELGDLDESKRAGSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHV 61

Query: 62  FIGNCYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILE 121
           FIGNCYSVSPEIVIRRFPNIRSVT+KGKPRFSDFNLVPPNWGADIHSWL++FAS+ PILE
Sbjct: 62  FIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVSFASRYPILE 121

Query: 122 ELRLKRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDI 181
           ELRLKRM VTDESLEFLARSFPNFK++SLMSCDGFSTDGLAAIATHCKNLT+LDILENDI
Sbjct: 122 ELRLKRMTVTDESLEFLARSFPNFKALSLMSCDGFSTDGLAAIATHCKNLTDLDILENDI 181

Query: 182 IDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQ 241
            DKSG+WLSCFPDTLKSLEVLNFASLNSDVSFEALEKLV+RCKSLKVLKVNRNINLEQLQ
Sbjct: 182 YDKSGSWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVKRCKSLKVLKVNRNINLEQLQ 241

Query: 242 RLLVHVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPAC 301
           RLLVH PQLTELGTGSF Q+ITLRQY DLE AFKNC NLHTLSGLLESTVLYLQVLFPAC
Sbjct: 242 RLLVHAPQLTELGTGSFSQEITLRQYYDLEHAFKNCNNLHTLSGLLESTVLYLQVLFPAC 301

Query: 302 TNLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPA 361
            NLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVG+SCPLLEELRVFPA
Sbjct: 302 ANLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFPA 361

Query: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421
           DPYADNLVHGVTESGFLAVSYGCRKLHY+LYFCRQMTNEAVATIVQNCPDFTHFRLCIMN
Sbjct: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYILYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421

Query: 422 PAQPDYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGK 481
           P QPDYLT Q MDEAFGAVVKTCSKLRRLAISGLLTDL FEYIGKYAKNLETLSVAFAG 
Sbjct: 422 PCQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGT 481

Query: 482 SDYGMQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAK 541
           SD+GMQCVM+GCPKLRKLEIRDSPFGNAALLSG+ERYESMRSLWMSACKVT NGCR+LAK
Sbjct: 482 SDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAK 541

Query: 542 QVPRLNVEVIK---GDNCGSDEAESVYVYRSVAGPRKDAPSCVLTL 580
           QVPRLNVEVIK   GD C   EAESVYVYRSVAG R+DAPS VLTL
Sbjct: 542 QVPRLNVEVIKAGGGDEC---EAESVYVYRSVAGRRRDAPSFVLTL 584

BLAST of MS010820 vs. ExPASy TrEMBL
Match: A0A6J1EM20 (protein AUXIN SIGNALING F-BOX 2-like OS=Cucurbita moschata OX=3662 GN=LOC111433791 PE=4 SV=1)

HSP 1 Score: 1073.2 bits (2774), Expect = 4.3e-310
Identity = 528/583 (90.57%), Postives = 549/583 (94.17%), Query Frame = 0

Query: 2   SKRKKKESGDSDESS-----FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHV 61
           S ++KKE GD DES      FPDEVLERVLSLVKSHKDRSSVSLVCK+W+NAERWSRTHV
Sbjct: 2   SSKRKKELGDLDESKRAGSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHV 61

Query: 62  FIGNCYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILE 121
           FIGNCYSVSPEIV+RRFPNIRSVT+KGKPRFSDFNLVPPNWGAD+HSWL++FASK PILE
Sbjct: 62  FIGNCYSVSPEIVVRRFPNIRSVTLKGKPRFSDFNLVPPNWGADVHSWLVSFASKYPILE 121

Query: 122 ELRLKRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDI 181
           ELRLKRM VTDESLEFLARSFPNFK++SLMSCDGFSTDGLAAIATHCKNLT+LDILENDI
Sbjct: 122 ELRLKRMTVTDESLEFLARSFPNFKALSLMSCDGFSTDGLAAIATHCKNLTDLDILENDI 181

Query: 182 IDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQ 241
            DKSG+WLSCFPDTLKSLEVLNFASLNSDVSFEALEKLV+RCKSLKVLKVNRNINLEQLQ
Sbjct: 182 YDKSGSWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVKRCKSLKVLKVNRNINLEQLQ 241

Query: 242 RLLVHVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPAC 301
           RLLVH PQLTELGTGSF Q+ITLRQY DLE AFKNC NLHTLSGLLESTVLYLQVLFPAC
Sbjct: 242 RLLVHAPQLTELGTGSFSQEITLRQYYDLEHAFKNCNNLHTLSGLLESTVLYLQVLFPAC 301

Query: 302 TNLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPA 361
            NLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVG+SCPLLEELRVFPA
Sbjct: 302 ANLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFPA 361

Query: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421
           DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN
Sbjct: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421

Query: 422 PAQPDYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGK 481
           P QPDYLT Q MDEAFGAVVKTCSKLRRLAISGLLTD  FEYIGKYAKNLETLSVAFAG 
Sbjct: 422 PCQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDSTFEYIGKYAKNLETLSVAFAGT 481

Query: 482 SDYGMQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAK 541
           SD+GMQCVM+GCPKLRKLEIRDSPFGNAALLSG+ERYESMRSLWMSACKVT NGCR+LAK
Sbjct: 482 SDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAK 541

Query: 542 QVPRLNVEVIKGDNCGSDEAESVYVYRSVAGPRKDAPSCVLTL 580
           QVPRLNVEVIK       EAESVYVYRSVAG R+DAPS VLTL
Sbjct: 542 QVPRLNVEVIKAGGSDECEAESVYVYRSVAGRRRDAPSFVLTL 584

BLAST of MS010820 vs. ExPASy TrEMBL
Match: A0A5D3CP23 (Protein AUXIN SIGNALING F-BOX 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007110 PE=4 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 2.8e-308
Identity = 523/583 (89.71%), Postives = 551/583 (94.51%), Query Frame = 0

Query: 2   SKRKKKESGDSDESS-----FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHV 61
           S +++K SGDSDES+     FPDEVLERVLSLVKSHKDRSSVSLVCK+W+NAERWSRTHV
Sbjct: 2   SSKRRKGSGDSDESNRAGSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHV 61

Query: 62  FIGNCYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILE 121
           FIGNCYSVSPEIVIRRFPNIRSVT+KGKPRFSDFNLVPPNWGAD+HSWL+AFASK PILE
Sbjct: 62  FIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADVHSWLVAFASKYPILE 121

Query: 122 ELRLKRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDI 181
           ELRLKRM VTDESLEFL+RSFPNFK++S+MSCDGFSTDGLAAIAT+CKNLTELDILENDI
Sbjct: 122 ELRLKRMTVTDESLEFLSRSFPNFKALSMMSCDGFSTDGLAAIATNCKNLTELDILENDI 181

Query: 182 IDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQ 241
            DKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQ
Sbjct: 182 NDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQ 241

Query: 242 RLLVHVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPAC 301
           RLLVH PQLTELG GSF Q+ITLRQYCDLE+AFK+CKNLHTLSGLLESTVLYLQVLFPAC
Sbjct: 242 RLLVHTPQLTELGMGSFSQEITLRQYCDLEEAFKSCKNLHTLSGLLESTVLYLQVLFPAC 301

Query: 302 TNLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPA 361
            N+TFLNLSYA LHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVG+SCPLLEELRVFPA
Sbjct: 302 ANITFLNLSYAILHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFPA 361

Query: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421
            P+ADNLVHGVTESGFLAVSYGCRKL YVLYFC QMTNEAVATIVQNCPDFTHFRLCIMN
Sbjct: 362 HPFADNLVHGVTESGFLAVSYGCRKLRYVLYFCHQMTNEAVATIVQNCPDFTHFRLCIMN 421

Query: 422 PAQPDYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGK 481
           P QPDYLT Q MDEAFGAVVKTCSKLRRLAISGLLTDL FEYIGKYAKNLETLSVAFAG 
Sbjct: 422 PHQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGS 481

Query: 482 SDYGMQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAK 541
           +D+GMQCVM+GCPKL+KLEIRDSPFGNAALLSG+ERYESMRSLWMSACKVT NGCR+LAK
Sbjct: 482 TDWGMQCVMSGCPKLKKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAK 541

Query: 542 QVPRLNVEVIKGDNCGSDEAESVYVYRSVAGPRKDAPSCVLTL 580
           QVPRLNVEVIK D     EAESVYVYRSVAGPR+DAP  V+TL
Sbjct: 542 QVPRLNVEVIKDDGNDECEAESVYVYRSVAGPRRDAPPFVVTL 584

BLAST of MS010820 vs. ExPASy TrEMBL
Match: A0A1S3B0L8 (protein AUXIN SIGNALING F-BOX 2-like OS=Cucumis melo OX=3656 GN=LOC103484554 PE=4 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 2.8e-308
Identity = 523/583 (89.71%), Postives = 551/583 (94.51%), Query Frame = 0

Query: 2   SKRKKKESGDSDESS-----FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHV 61
           S +++K SGDSDES+     FPDEVLERVLSLVKSHKDRSSVSLVCK+W+NAERWSRTHV
Sbjct: 2   SSKRRKGSGDSDESNRAGSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHV 61

Query: 62  FIGNCYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILE 121
           FIGNCYSVSPEIVIRRFPNIRSVT+KGKPRFSDFNLVPPNWGAD+HSWL+AFASK PILE
Sbjct: 62  FIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADVHSWLVAFASKYPILE 121

Query: 122 ELRLKRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDI 181
           ELRLKRM VTDESLEFL+RSFPNFK++S+MSCDGFSTDGLAAIAT+CKNLTELDILENDI
Sbjct: 122 ELRLKRMTVTDESLEFLSRSFPNFKALSMMSCDGFSTDGLAAIATNCKNLTELDILENDI 181

Query: 182 IDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQ 241
            DKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQ
Sbjct: 182 NDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQ 241

Query: 242 RLLVHVPQLTELGTGSFLQKITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPAC 301
           RLLVH PQLTELG GSF Q+ITLRQYCDLE+AFK+CKNLHTLSGLLESTVLYLQVLFPAC
Sbjct: 242 RLLVHTPQLTELGMGSFSQEITLRQYCDLEEAFKSCKNLHTLSGLLESTVLYLQVLFPAC 301

Query: 302 TNLTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPA 361
            N+TFLNLSYA LHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVG+SCPLLEELRVFPA
Sbjct: 302 ANITFLNLSYAILHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFPA 361

Query: 362 DPYADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMN 421
            P+ADNLVHGVTESGFLAVSYGCRKL YVLYFC QMTNEAVATIVQNCPDFTHFRLCIMN
Sbjct: 362 HPFADNLVHGVTESGFLAVSYGCRKLRYVLYFCHQMTNEAVATIVQNCPDFTHFRLCIMN 421

Query: 422 PAQPDYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGK 481
           P QPDYLT Q MDEAFGAVVKTCSKLRRLAISGLLTDL FEYIGKYAKNLETLSVAFAG 
Sbjct: 422 PHQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGS 481

Query: 482 SDYGMQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAK 541
           +D+GMQCVM+GCPKL+KLEIRDSPFGNAALLSG+ERYESMRSLWMSACKVT NGCR+LAK
Sbjct: 482 TDWGMQCVMSGCPKLKKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAK 541

Query: 542 QVPRLNVEVIKGDNCGSDEAESVYVYRSVAGPRKDAPSCVLTL 580
           QVPRLNVEVIK D     EAESVYVYRSVAGPR+DAP  V+TL
Sbjct: 542 QVPRLNVEVIKDDGNDECEAESVYVYRSVAGPRRDAPPFVVTL 584

BLAST of MS010820 vs. TAIR 10
Match: AT3G62980.1 (F-box/RNI-like superfamily protein )

HSP 1 Score: 632.1 bits (1629), Expect = 4.4e-181
Identity = 314/568 (55.28%), Postives = 396/568 (69.72%), Query Frame = 0

Query: 16  SFPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGNCYSVSPEIVIRRFPN 75
           SFP+EVLE V S ++  KDR+SVSLVCK WY  ERW R  VFIGNCY+VSP  VIRRFP 
Sbjct: 8   SFPEEVLEHVFSFIQLDKDRNSVSLVCKSWYEIERWCRRKVFIGNCYAVSPATVIRRFPK 67

Query: 76  IRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRLKRMAVTDESLEFLAR 135
           +RSV +KGKP F+DFNLVP  WG  ++ W+ A +S    LEE+RLKRM VTD+ LE +A+
Sbjct: 68  VRSVELKGKPHFADFNLVPDGWGGYVYPWIEAMSSSYTWLEEIRLKRMVVTDDCLELIAK 127

Query: 136 SFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLE 195
           SF NFK + L SC+GFSTDGLAAIA  C+NL ELD+ E+D+ D SG+WLS FPDT  SL 
Sbjct: 128 SFKNFKVLVLSSCEGFSTDGLAAIAATCRNLKELDLRESDVDDVSGHWLSHFPDTYTSLV 187

Query: 196 VLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHVPQLTELGTGSFLQ 255
            LN + L S+VSF ALE+LV RC +LK LK+NR + LE+L  LL   PQL ELGTG +  
Sbjct: 188 SLNISCLASEVSFSALERLVTRCPNLKSLKLNRAVPLEKLATLLQRAPQLEELGTGGYTA 247

Query: 256 KITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLTFLNLSYATLHGGELA 315
           ++    Y  L  A   CK L  LSG  ++   YL  ++  C+ LT LNLSYAT+   +L 
Sbjct: 248 EVRPDVYSGLSVALSGCKELRCLSGFWDAVPAYLPAVYSVCSRLTTLNLSYATVQSYDLV 307

Query: 316 GLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYADNLVHGVTESGFLAV 375
            LL  CP L+RLWVLD +ED GL+ +  +C  L ELRVFP++P+       +TE G ++V
Sbjct: 308 KLLCQCPKLQRLWVLDYIEDAGLEVLASTCKDLRELRVFPSEPFVMEPNVALTEQGLVSV 367

Query: 376 SYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQPDYLTNQSMDEAFGAV 435
           S GC KL  VLYFCRQMTN A+ TI +N P+ T FRLCI+ P  PDYLT + +D  FGA+
Sbjct: 368 SMGCPKLESVLYFCRQMTNAALITIARNRPNMTRFRLCIIEPKAPDYLTLEPLDIGFGAI 427

Query: 436 VKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYGMQCVMNGCPKLRKLE 495
           V+ C  LRRL++SGLLTD  FEYIG YAK +E LSVAFAG SD GM  V++GC  LRKLE
Sbjct: 428 VEHCKDLRRLSLSGLLTDKVFEYIGTYAKKMEMLSVAFAGDSDLGMHHVLSGCDSLRKLE 487

Query: 496 IRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPRLNVEVIKGDNCGSDE 555
           IRD PFG+ ALL+   + E+MRSLWMS+C V+   C++L +++P+LNVEVI  D  G+ +
Sbjct: 488 IRDCPFGDKALLANASKLETMRSLWMSSCSVSFGACKLLGQKMPKLNVEVI--DERGAPD 547

Query: 556 A-------ESVYVYRSVAGPRKDAPSCV 577
           +       E V++YR+VAGPR D P  V
Sbjct: 548 SRPESCPVERVFIYRTVAGPRFDMPGFV 573

BLAST of MS010820 vs. TAIR 10
Match: AT3G26810.1 (auxin signaling F-box 2 )

HSP 1 Score: 632.1 bits (1629), Expect = 4.4e-181
Identity = 308/573 (53.75%), Postives = 402/573 (70.16%), Query Frame = 0

Query: 17  FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 76
           FPDEV+E V   V SHKDR+++SLVCK WY  ER+SR  VFIGNCY+++PE ++RRFP +
Sbjct: 4   FPDEVIEHVFDFVTSHKDRNAISLVCKSWYKIERYSRQKVFIGNCYAINPERLLRRFPCL 63

Query: 77  RSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRLKRMAVTDESLEFLARS 136
           +S+T+KGKP F+DFNLVP  WG  +  W+ A A     LEELRLKRM VTDESLE L+RS
Sbjct: 64  KSLTLKGKPHFADFNLVPHEWGGFVLPWIEALARSRVGLEELRLKRMVVTDESLELLSRS 123

Query: 137 FPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 196
           F NFKS+ L+SC+GF+TDGLA+IA +C++L +LD+ EN+I D  G WLSCFPDT  +L  
Sbjct: 124 FVNFKSLVLVSCEGFTTDGLASIAANCRHLRDLDLQENEIDDHRGQWLSCFPDTCTTLVT 183

Query: 197 LNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHVPQLTELGTGSFLQK 256
           LNFA L  + +  ALE+LV R  +LK LK+NR + L+ L RL+   PQ+ +LG GS+   
Sbjct: 184 LNFACLEGETNLVALERLVARSPNLKSLKLNRAVPLDALARLMACAPQIVDLGVGSYEND 243

Query: 257 ITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLTFLNLSYAT-LHGGELA 316
                Y  L    K C +L +LSG LE+    L    P C NLT LNLSYA  +HG  L 
Sbjct: 244 PDSESYLKLMAVIKKCTSLRSLSGFLEAAPHCLSAFHPICHNLTSLNLSYAAEIHGSHLI 303

Query: 317 GLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYADNLVHGVTESGFLAV 376
            L+ HC  L+RLW+LD++ DKGL+ V  +C  L+ELRVFP+D         VTE G +A+
Sbjct: 304 KLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQELRVFPSDLLGGGNT-AVTEEGLVAI 363

Query: 377 SYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQPDYLTNQSMDEAFGAV 436
           S GC KLH +LYFC+QMTN A+ T+ +NCP+F  FRLCI+ P +PD++T+Q +DE FGA+
Sbjct: 364 SAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRLCILEPNKPDHVTSQPLDEGFGAI 423

Query: 437 VKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYGMQCVMNGCPKLRKLE 496
           VK C  LRRL++SGLLTD  F YIG YA  LE LS+AFAG +D GM  V+NGC K++KLE
Sbjct: 424 VKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSIAFAGDTDKGMLYVLNGCKKMKKLE 483

Query: 497 IRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPRLNVEVI-KGDNC--- 556
           IRDSPFG+ ALL+ + +YE+MRSLWMS+C+VT +GC+ LA++ P LNVE+I + DN    
Sbjct: 484 IRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSGCKRLAEKAPWLNVEIINENDNNRME 543

Query: 557 -----GSDEAESVYVYRSVAGPRKDAPSCVLTL 580
                G  + + +Y+YR+V G R DAP  V  L
Sbjct: 544 ENGHEGRQKVDKLYLYRTVVGTRMDAPPFVWIL 575

BLAST of MS010820 vs. TAIR 10
Match: AT1G12820.1 (auxin signaling F-box 3 )

HSP 1 Score: 630.2 bits (1624), Expect = 1.7e-180
Identity = 316/575 (54.96%), Postives = 406/575 (70.61%), Query Frame = 0

Query: 17  FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 76
           FPDEV+E V   V SHKDR+S+SLVCK W+  ER+SR  VFIGNCY+++PE +IRRFP +
Sbjct: 4   FPDEVIEHVFDFVASHKDRNSISLVCKSWHKIERFSRKEVFIGNCYAINPERLIRRFPCL 63

Query: 77  RSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRLKRMAVTDESLEFLARS 136
           +S+T+KGKP F+DFNLVP  WG  +H W+ A A     LEELRLKRM VTDESL+ L+RS
Sbjct: 64  KSLTLKGKPHFADFNLVPHEWGGFVHPWIEALARSRVGLEELRLKRMVVTDESLDLLSRS 123

Query: 137 FPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 196
           F NFKS+ L+SC+GF+TDGLA+IA +C++L ELD+ EN+I D  G WL+CFPD+  +L  
Sbjct: 124 FANFKSLVLVSCEGFTTDGLASIAANCRHLRELDLQENEIDDHRGQWLNCFPDSCTTLMS 183

Query: 197 LNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHVPQLTELGTGSFLQK 256
           LNFA L  + +  ALE+LV R  +LK LK+NR + L+ L RL+   PQL +LG GS+  +
Sbjct: 184 LNFACLKGETNVAALERLVARSPNLKSLKLNRAVPLDALARLMSCAPQLVDLGVGSYENE 243

Query: 257 ITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLTFLNLSYAT-LHGGELA 316
                +  L  A K   +L +LSG LE   L L   +P C NL  LNLSYA  + G  L 
Sbjct: 244 PDPESFAKLMTAIKKYTSLRSLSGFLEVAPLCLPAFYPICQNLISLNLSYAAEIQGNHLI 303

Query: 317 GLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYA--DNLVHGVTESGFL 376
            L+  C  L+RLW+LD++ DKGL  V  +C  L+ELRVFP+D +   DN    VTE G +
Sbjct: 304 KLIQLCKRLQRLWILDSIGDKGLAVVAATCKELQELRVFPSDVHGEEDNNA-SVTEVGLV 363

Query: 377 AVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQPDYLTNQSMDEAFG 436
           A+S GC KLH +LYFC+QMTN A+  + +NCP+F  FRLCI+ P +PD++T QS+DE FG
Sbjct: 364 AISAGCPKLHSILYFCKQMTNAALIAVAKNCPNFIRFRLCILEPHKPDHITFQSLDEGFG 423

Query: 437 AVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYGMQCVMNGCPKLRK 496
           A+V+ C  LRRL++SGLLTD  F YIG YA+ LE LS+AFAG +D GM  V+NGC K+RK
Sbjct: 424 AIVQACKGLRRLSVSGLLTDQVFLYIGMYAEQLEMLSIAFAGDTDKGMLYVLNGCKKMRK 483

Query: 497 LEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPRLNVEVI-KGDNCG 556
           LEIRDSPFGNAALL+ + RYE+MRSLWMS+C+VT  GC+ LA+  PRLNVE+I + +N G
Sbjct: 484 LEIRDSPFGNAALLADVGRYETMRSLWMSSCEVTLGGCKRLAQNSPRLNVEIINENENNG 543

Query: 557 -----SDEAESV---YVYRSVAGPRKDAPSCVLTL 580
                 DE E V   Y+YR+V G RKDAP  V  L
Sbjct: 544 MEQNEEDEREKVDKLYLYRTVVGTRKDAPPYVRIL 577

BLAST of MS010820 vs. TAIR 10
Match: AT4G03190.1 (GRR1-like protein 1 )

HSP 1 Score: 579.7 bits (1493), Expect = 2.6e-165
Identity = 290/567 (51.15%), Postives = 385/567 (67.90%), Query Frame = 0

Query: 17  FPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 76
           FP +VLE +LS + S++DR+SVSLVCK W+  ER +R  VF+GNCY+VSP  V RRFP +
Sbjct: 5   FPPKVLEHILSFIDSNEDRNSVSLVCKSWFETERKTRKRVFVGNCYAVSPAAVTRRFPEM 64

Query: 77  RSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRLKRMAVTDESLEFLARS 136
           RS+T+KGKP F+D+NLVP  WG     W+ A A+KS  LEE+R+KRM VTDE LE +A S
Sbjct: 65  RSLTLKGKPHFADYNLVPDGWGGYAWPWIEAMAAKSSSLEEIRMKRMVVTDECLEKIAAS 124

Query: 137 FPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 196
           F +FK + L SC+GFSTDG+AAIA  C+NL  L++ E  + D  G+WLS FP++  SL  
Sbjct: 125 FKDFKVLVLTSCEGFSTDGIAAIAATCRNLRVLELRECIVEDLGGDWLSYFPESSTSLVS 184

Query: 197 LNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHVPQLTELGTGSFLQK 256
           L+F+ L+S+V    LE+LV R  +LK LK+N  + L+ L  LL   PQLTELGTGSF  +
Sbjct: 185 LDFSCLDSEVKISDLERLVSRSPNLKSLKLNPAVTLDGLVSLLRCAPQLTELGTGSFAAQ 244

Query: 257 ITLRQYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTNLTFLNLSYATLHGGELAG 316
           +    +  L +AF NCK L +LSGL +    YL  L+  C  LT LNLSYAT+   +L  
Sbjct: 245 LKPEAFSKLSEAFSNCKQLQSLSGLWDVLPEYLPALYSVCPGLTSLNLSYATVRMPDLVE 304

Query: 317 LLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADPYADNLVHGVTESGFLAVS 376
           LL  C  L++LWV+D +EDKGL+AV   C  L ELRVFP++P  D     +TE G + VS
Sbjct: 305 LLRRCSKLQKLWVMDLIEDKGLEAVASYCKELRELRVFPSEPDLDATNIPLTEQGLVFVS 364

Query: 377 YGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPAQPDYLTNQSMDEAFGAVV 436
            GCRKL  VLYFC Q TN A+ TI +  P+   FRLC++ P  PDY TN+ +D+ F A+ 
Sbjct: 365 KGCRKLESVLYFCVQFTNAALFTIARKRPNLKCFRLCVIEPFAPDYKTNEPLDKGFKAIA 424

Query: 437 KTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSDYGMQCVMNGCPKLRKLEI 496
           + C  LRRL++SGLL+D AF+YIGK+AK +  LS+AFAG SD  +  +++GC  L+KLEI
Sbjct: 425 EGCRDLRRLSVSGLLSDKAFKYIGKHAKKVRMLSIAFAGDSDLMLHHLLSGCESLKKLEI 484

Query: 497 RDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQVPRLNVEVI----KGDNCG 556
           RD PFG+ ALL    + E+MRSLWMS+C V+   C++L++++PRLNVEVI          
Sbjct: 485 RDCPFGDTALLEHAAKLETMRSLWMSSCFVSFGACKLLSQKMPRLNVEVIDEHPPESRPE 544

Query: 557 SDEAESVYVYRSVAGPRKDAPSCVLTL 580
           S   E +Y+YR+VAGPR D P  V T+
Sbjct: 545 SSPVERIYIYRTVAGPRMDTPEFVWTI 571

BLAST of MS010820 vs. TAIR 10
Match: AT4G24390.2 (RNI-like superfamily protein )

HSP 1 Score: 566.2 bits (1458), Expect = 3.0e-161
Identity = 286/587 (48.72%), Postives = 380/587 (64.74%), Query Frame = 0

Query: 1   NSKRKKKESGDSDESSFPDEVLERVLSLVKSHKDRSSVSLVCKEWYNAERWSRTHVFIGN 60
           N  R  K S         + VLE VL  + S  DR++VSLVC+ WY  E  +R  VFIGN
Sbjct: 38  NKSRNFKSSPPPCPDHVLENVLENVLQFLTSRCDRNAVSLVCRSWYRVEAQTRLEVFIGN 97

Query: 61  CYSVSPEIVIRRFPNIRSVTIKGKPRFSDFNLVPPNWGADIHSWLLAFASKSPILEELRL 120
           CYS+SP  +I RF  +RS+ +KGKPRF+DFNL+PPNWGA    W+ A A   P LE++ L
Sbjct: 98  CYSLSPARLIHRFKRVRSLVLKGKPRFADFNLMPPNWGAQFSPWVAATAKAYPWLEKVHL 157

Query: 121 KRMAVTDESLEFLARSFPNFKSISLMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKS 180
           KRM VTD+ L  LA SFP FK ++L+ C+GF T G+A +A  C+ L  LD++E+++ D  
Sbjct: 158 KRMFVTDDDLALLAESFPGFKELTLVCCEGFGTSGIAIVANKCRQLKVLDLMESEVTDDE 217

Query: 181 GNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLV 240
            +W+SCFP+    LE L+F  + S ++F+ALE+LV R   LK L+ NR ++LE+L RL+V
Sbjct: 218 LDWISCFPEGETHLESLSFDCVESPINFKALEELVVRSPFLKKLRTNRFVSLEELHRLMV 277

Query: 241 HVPQLTELGTGSFLQKITLR--QYCDLEDAFKNCKNLHTLSGLLESTVLYLQVLFPACTN 300
             PQLT LGTGSF      +  Q  D   AF+ CK++  LSG  E    YL  +   C N
Sbjct: 278 RAPQLTSLGTGSFSPDNVPQGEQQPDYAAAFRACKSIVCLSGFREFRPEYLLAISSVCAN 337

Query: 301 LTFLNLSYATLHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGISCPLLEELRVFPADP 360
           LT LN SYA +    L  ++S+C  +R  W LD++ D+GL+AV  +C  L ELR+FP DP
Sbjct: 338 LTSLNFSYANISPHMLKPIISNCHNIRVFWALDSIRDEGLQAVAATCKELRELRIFPFDP 397

Query: 361 YADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPA 420
             D+    V+  G  A+S GCRKL  +LYFC+ MTN AV  + +NCP  T FRLCIM   
Sbjct: 398 REDS-EGPVSGVGLQAISEGCRKLESILYFCQNMTNGAVTAMSENCPQLTVFRLCIMGRH 457

Query: 421 QPDYLTNQSMDEAFGAVVKTCSKLRRLAISGLLTDLAFEYIGKYAKNLETLSVAFAGKSD 480
           +PD++T + MD+ FGA+VK C KL RLA+SGLLTD AF YIG+Y K + TLSVAFAG SD
Sbjct: 458 RPDHVTGKPMDDGFGAIVKNCKKLTRLAVSGLLTDEAFSYIGEYGKLIRTLSVAFAGNSD 517

Query: 481 YGMQCVMNGCPKLRKLEIRDSPFGNAALLSGMERYESMRSLWMSACKVTRNGCRILAKQV 540
             ++ V+ GCPKL+KLEIRDSPFG+  L SGM RY +MR +W+S+C ++R GCR ++  +
Sbjct: 518 KALRYVLEGCPKLQKLEIRDSPFGDVGLRSGMHRYSNMRFVWLSSCLISRGGCRGVSHAL 577

Query: 541 PRLNVEVIKG------DNCGSDEAESVYVYRSVAGPRKDAPSCVLTL 580
           P + VEV         D    D  E++Y+YRS+ GPRKDAP  V  L
Sbjct: 578 PNVVVEVFGADGDDDEDTVTGDYVETLYLYRSLDGPRKDAPKFVTIL 623

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142182.10.0e+0099.65protein TRANSPORT INHIBITOR RESPONSE 1-like [Momordica charantia] >XP_022142183.... [more]
XP_038883074.14.4e-31090.39protein TRANSPORT INHIBITOR RESPONSE 1-like [Benincasa hispida][more]
XP_023002910.14.4e-31090.78protein AUXIN SIGNALING F-BOX 2-like [Cucurbita maxima][more]
XP_023518017.14.4e-31090.74protein AUXIN SIGNALING F-BOX 2-like [Cucurbita pepo subsp. pepo][more]
XP_022926770.18.9e-31090.57protein AUXIN SIGNALING F-BOX 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LW296.2e-18053.75Protein AUXIN SIGNALING F-BOX 2 OS=Arabidopsis thaliana OX=3702 GN=AFB2 PE=1 SV=... [more]
Q570C06.2e-18055.28Protein TRANSPORT INHIBITOR RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=TIR1 P... [more]
Q9LPW72.4e-17954.96Protein AUXIN SIGNALING F-BOX 3 OS=Arabidopsis thaliana OX=3702 GN=AFB3 PE=1 SV=... [more]
Q7XVM82.6e-17853.66Transport inhibitor response 1-like protein Os04g0395600 OS=Oryza sativa subsp. ... [more]
Q9ZR123.6e-16451.15GRR1-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=GRH1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CM080.0e+0099.65protein TRANSPORT INHIBITOR RESPONSE 1-like OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1KQA42.2e-31090.78protein AUXIN SIGNALING F-BOX 2-like OS=Cucurbita maxima OX=3661 GN=LOC111496678... [more]
A0A6J1EM204.3e-31090.57protein AUXIN SIGNALING F-BOX 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114337... [more]
A0A5D3CP232.8e-30889.71Protein AUXIN SIGNALING F-BOX 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3B0L82.8e-30889.71protein AUXIN SIGNALING F-BOX 2-like OS=Cucumis melo OX=3656 GN=LOC103484554 PE=... [more]
Match NameE-valueIdentityDescription
AT3G62980.14.4e-18155.28F-box/RNI-like superfamily protein [more]
AT3G26810.14.4e-18153.75auxin signaling F-box 2 [more]
AT1G12820.11.7e-18054.96auxin signaling F-box 3 [more]
AT4G03190.12.6e-16551.15GRR1-like protein 1 [more]
AT4G24390.23.0e-16148.72RNI-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006553Leucine-rich repeat, cysteine-containing subtypeSMARTSM00367LRR_CC_2coord: 137..162
e-value: 83.0
score: 7.4
coord: 439..462
e-value: 28.0
score: 11.0
coord: 379..403
e-value: 19.0
score: 12.4
coord: 112..136
e-value: 220.0
score: 4.0
coord: 488..512
e-value: 330.0
score: 2.7
coord: 463..487
e-value: 580.0
score: 0.8
IPR001810F-box domainSMARTSM00256fbox_2coord: 17..58
e-value: 4.2E-4
score: 29.6
IPR041101Transport inhibitor response 1 domainPFAMPF18791Transp_inhibitcoord: 74..120
e-value: 5.5E-21
score: 73.8
IPR041567COI1, F-boxPFAMPF18511F-box_5coord: 15..55
e-value: 5.3E-21
score: 73.9
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 54..579
e-value: 4.3E-166
score: 555.3
NoneNo IPR availableGENE3D1.20.1280.50coord: 14..53
e-value: 2.3E-17
score: 64.2
NoneNo IPR availablePANTHERPTHR16134F-BOX/TPR REPEAT PROTEIN POF3coord: 11..578
NoneNo IPR availablePANTHERPTHR16134:SF37PROTEIN AUXIN SIGNALING F-BOX 3-LIKEcoord: 11..578
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 94..527
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 10..49

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010820.1MS010820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding