MS010790 (gene) Bitter gourd (TR) v1

Overview
NameMS010790
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPectinesterase
Locationscaffold35: 1983283 .. 1985047 (-)
RNA-Seq ExpressionMS010790
SyntenyMS010790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACCAATCAGCCGCTCCTCCAACCCCCCAAAACTCACCGCTCCAAAGCCTTCTCCATAATCCTCTGTTTCGCTACCATTTTATGCTCAGCTTTCTTCATCGCCAACCACTTGATCGGACCAAACCCATCTGAAATTGATCAGCTCTTCCTCCATAACATCTGCCGTAAGGCTTATAACCCAGCATCATGCGTTGCAATGGCCGCCTCCGAAGTTCCCTTCCATCGAGAAGCGAAAATGACCAATGGCGTTCATTTCTTACAGGTTTTCTTGAGGAAATCCCTGACCAAAGTTACGGACACGATCGAGAGAGCGAATCATCTCATTCTCAGCCGTAGGATCAACGACGCGCGTGGGGAAGCAGCGCTGGCGGACTGCCTGGAGCTCATGGAGATCTCCATGGACCGAATAACGGACTCCATTTTGGCTCTGGCTCTGAACAACAAAACGAGTGGGCCCATCGAAGATGCCCACGCGTGGCTCAGTAGCGTGCTCACGAACCACGTCACGTGCTCCGATGAGTTGGAGAAGTCACTGCTCGGAACCACGGCGGCGGAGCTAGGGCTGGAGGAGTTGATTATGACGGCGAGAAACTCTCTGGCCATGTTGGTTTCGATTTCGCAACCGGAACCGGAACCCGAAATTGAGGGACAAATTGGGTTTCCGGGATGGCTGAAGAGTGGGGATCGGATGCTTTTGGGGGTCACGGGCCAGCAAATCAAAGCCAATATAGTGGTGGCCAAGGATGGGAGTGGGAATTTCAAGACGGTGAAGGAGGCGGTTGCGTCGGTTCCAGATAAAAGTAAGAACAGATTTGTGATATACGTGAAGAAGGGAACTTACGAGGAGAATGTAGAAGTCGAAAAGAAGAAGAAGAATGTGATGATCGTTGGAGATGGAATGGATTCCACCATCATCACTGGCAGTCTCAATGTTGTTGACGGATCTACAACTTTTAAATCTGCAACTGTAGGTAAAAACCTTCTCACTGACTCATTAGAAAATTTTAAAATAGCGATTCATGAACAAACTATGATTTAATCTCATGGGGTTTAATTTGATTGATGATCCAGCTGCCGTGGGAGATGGATTTATAGCCCAAGATATCTGGTTCAGGAACACCGCCGGGCCTGAAAAGCACCAAGCGGTGGCACTTCGCGTCGGAGCCGACCAATCCGTCATTAACCGGTGTCGTATCGACGCTTACCAAGACACTCTATACACACATTCCAACCGCCAATTCTACAGAGACTCCACAATCACCGGCACCGTCGACTTCATATTCGGGAACGCCGCCGTCGTACTACAGAACTGCAAAATAGAAGCCCGTCGGCCCATGAGCAACCAGGCCAACATGGTTACGGCCCAAGGCCGAATCGATCCGAATCAGAACACTGGAACTTCCATCCACAGATGCCAAATCGTGGCAAGCTCTGACCTCGAGCCCGTTAAACGGTCCATTAAGACATATTTGGGCCGCCCATGGAAGGAGTATTCGAGGACCGTCATCATGCAGTCCAGAATTGGAGATCTTATAGATCCGGCGGGCTGGGCCGAGTGGAATGGGGACTTTGCACTGAAGACGCTATATTACGGTGAGTATTTGAATAATGGGCCGGGCTCTGATGTTAGCAAGCGAGTCAAATGGCCCGGTTACCGTGTCATTACCAACCCGGCTGAGGCCCAAAAGTTCACGGTGGATTCACTAATACAAGGAGGTGATTGGCTGGGCCCCACAGGCGTGACTTTCATCCCAGGGCTG

mRNA sequence

ATGGCGACCAATCAGCCGCTCCTCCAACCCCCCAAAACTCACCGCTCCAAAGCCTTCTCCATAATCCTCTGTTTCGCTACCATTTTATGCTCAGCTTTCTTCATCGCCAACCACTTGATCGGACCAAACCCATCTGAAATTGATCAGCTCTTCCTCCATAACATCTGCCGTAAGGCTTATAACCCAGCATCATGCGTTGCAATGGCCGCCTCCGAAGTTCCCTTCCATCGAGAAGCGAAAATGACCAATGGCGTTCATTTCTTACAGGTTTTCTTGAGGAAATCCCTGACCAAAGTTACGGACACGATCGAGAGAGCGAATCATCTCATTCTCAGCCGTAGGATCAACGACGCGCGTGGGGAAGCAGCGCTGGCGGACTGCCTGGAGCTCATGGAGATCTCCATGGACCGAATAACGGACTCCATTTTGGCTCTGGCTCTGAACAACAAAACGAGTGGGCCCATCGAAGATGCCCACGCGTGGCTCAGTAGCGTGCTCACGAACCACGTCACGTGCTCCGATGAGTTGGAGAAGTCACTGCTCGGAACCACGGCGGCGGAGCTAGGGCTGGAGGAGTTGATTATGACGGCGAGAAACTCTCTGGCCATGTTGGTTTCGATTTCGCAACCGGAACCGGAACCCGAAATTGAGGGACAAATTGGGTTTCCGGGATGGCTGAAGAGTGGGGATCGGATGCTTTTGGGGGTCACGGGCCAGCAAATCAAAGCCAATATAGTGGTGGCCAAGGATGGGAGTGGGAATTTCAAGACGGTGAAGGAGGCGGTTGCGTCGGTTCCAGATAAAAGTAAGAACAGATTTGTGATATACGTGAAGAAGGGAACTTACGAGGAGAATGTAGAAGTCGAAAAGAAGAAGAAGAATGTGATGATCGTTGGAGATGGAATGGATTCCACCATCATCACTGGCAGTCTCAATGTTGTTGACGGATCTACAACTTTTAAATCTGCAACTGTAGCTGCCGTGGGAGATGGATTTATAGCCCAAGATATCTGGTTCAGGAACACCGCCGGGCCTGAAAAGCACCAAGCGGTGGCACTTCGCGTCGGAGCCGACCAATCCGTCATTAACCGGTGTCGTATCGACGCTTACCAAGACACTCTATACACACATTCCAACCGCCAATTCTACAGAGACTCCACAATCACCGGCACCGTCGACTTCATATTCGGGAACGCCGCCGTCGTACTACAGAACTGCAAAATAGAAGCCCGTCGGCCCATGAGCAACCAGGCCAACATGGTTACGGCCCAAGGCCGAATCGATCCGAATCAGAACACTGGAACTTCCATCCACAGATGCCAAATCGTGGCAAGCTCTGACCTCGAGCCCGTTAAACGGTCCATTAAGACATATTTGGGCCGCCCATGGAAGGAGTATTCGAGGACCGTCATCATGCAGTCCAGAATTGGAGATCTTATAGATCCGGCGGGCTGGGCCGAGTGGAATGGGGACTTTGCACTGAAGACGCTATATTACGGTGAGTATTTGAATAATGGGCCGGGCTCTGATGTTAGCAAGCGAGTCAAATGGCCCGGTTACCGTGTCATTACCAACCCGGCTGAGGCCCAAAAGTTCACGGTGGATTCACTAATACAAGGAGGTGATTGGCTGGGCCCCACAGGCGTGACTTTCATCCCAGGGCTG

Coding sequence (CDS)

ATGGCGACCAATCAGCCGCTCCTCCAACCCCCCAAAACTCACCGCTCCAAAGCCTTCTCCATAATCCTCTGTTTCGCTACCATTTTATGCTCAGCTTTCTTCATCGCCAACCACTTGATCGGACCAAACCCATCTGAAATTGATCAGCTCTTCCTCCATAACATCTGCCGTAAGGCTTATAACCCAGCATCATGCGTTGCAATGGCCGCCTCCGAAGTTCCCTTCCATCGAGAAGCGAAAATGACCAATGGCGTTCATTTCTTACAGGTTTTCTTGAGGAAATCCCTGACCAAAGTTACGGACACGATCGAGAGAGCGAATCATCTCATTCTCAGCCGTAGGATCAACGACGCGCGTGGGGAAGCAGCGCTGGCGGACTGCCTGGAGCTCATGGAGATCTCCATGGACCGAATAACGGACTCCATTTTGGCTCTGGCTCTGAACAACAAAACGAGTGGGCCCATCGAAGATGCCCACGCGTGGCTCAGTAGCGTGCTCACGAACCACGTCACGTGCTCCGATGAGTTGGAGAAGTCACTGCTCGGAACCACGGCGGCGGAGCTAGGGCTGGAGGAGTTGATTATGACGGCGAGAAACTCTCTGGCCATGTTGGTTTCGATTTCGCAACCGGAACCGGAACCCGAAATTGAGGGACAAATTGGGTTTCCGGGATGGCTGAAGAGTGGGGATCGGATGCTTTTGGGGGTCACGGGCCAGCAAATCAAAGCCAATATAGTGGTGGCCAAGGATGGGAGTGGGAATTTCAAGACGGTGAAGGAGGCGGTTGCGTCGGTTCCAGATAAAAGTAAGAACAGATTTGTGATATACGTGAAGAAGGGAACTTACGAGGAGAATGTAGAAGTCGAAAAGAAGAAGAAGAATGTGATGATCGTTGGAGATGGAATGGATTCCACCATCATCACTGGCAGTCTCAATGTTGTTGACGGATCTACAACTTTTAAATCTGCAACTGTAGCTGCCGTGGGAGATGGATTTATAGCCCAAGATATCTGGTTCAGGAACACCGCCGGGCCTGAAAAGCACCAAGCGGTGGCACTTCGCGTCGGAGCCGACCAATCCGTCATTAACCGGTGTCGTATCGACGCTTACCAAGACACTCTATACACACATTCCAACCGCCAATTCTACAGAGACTCCACAATCACCGGCACCGTCGACTTCATATTCGGGAACGCCGCCGTCGTACTACAGAACTGCAAAATAGAAGCCCGTCGGCCCATGAGCAACCAGGCCAACATGGTTACGGCCCAAGGCCGAATCGATCCGAATCAGAACACTGGAACTTCCATCCACAGATGCCAAATCGTGGCAAGCTCTGACCTCGAGCCCGTTAAACGGTCCATTAAGACATATTTGGGCCGCCCATGGAAGGAGTATTCGAGGACCGTCATCATGCAGTCCAGAATTGGAGATCTTATAGATCCGGCGGGCTGGGCCGAGTGGAATGGGGACTTTGCACTGAAGACGCTATATTACGGTGAGTATTTGAATAATGGGCCGGGCTCTGATGTTAGCAAGCGAGTCAAATGGCCCGGTTACCGTGTCATTACCAACCCGGCTGAGGCCCAAAAGTTCACGGTGGATTCACTAATACAAGGAGGTGATTGGCTGGGCCCCACAGGCGTGACTTTCATCCCAGGGCTG

Protein sequence

MATNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRKAYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDARGEAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELEKSLLGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGDLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLIQGGDWLGPTGVTFIPGL
Homology
BLAST of MS010790 vs. NCBI nr
Match: XP_022142368.1 (pectinesterase [Momordica charantia])

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 552/555 (99.46%), Postives = 552/555 (99.46%), Query Frame = 0

Query: 1   MATNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRKAY 60
           MATNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRKAY
Sbjct: 1   MATNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRKAY 60

Query: 61  NPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDARG 120
           NPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERAN LILSRRINDARG
Sbjct: 61  NPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANDLILSRRINDARG 120

Query: 121 EAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELEKSL 180
           EAALADCLELMEISMDRITDSILALALNNKTS PIEDAHAWLSSVLTNHVTCSDELEKSL
Sbjct: 121 EAALADCLELMEISMDRITDSILALALNNKTSVPIEDAHAWLSSVLTNHVTCSDELEKSL 180

Query: 181 LGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTGQQ 240
           LGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTGQQ
Sbjct: 181 LGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTGQQ 240

Query: 241 IKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIVGD 300
           IKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIVGD
Sbjct: 241 IKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGDLI 480

Query: 481 DPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLIQG 540
           DP GWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLIQG
Sbjct: 481 DPTGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLIQG 540

Query: 541 GDWLGPTGVTFIPGL 556
           GDWLGPTGVTFIPGL
Sbjct: 541 GDWLGPTGVTFIPGL 555

BLAST of MS010790 vs. NCBI nr
Match: XP_038881670.1 (pectinesterase [Benincasa hispida])

HSP 1 Score: 867.1 bits (2239), Expect = 8.4e-248
Identity = 440/558 (78.85%), Postives = 479/558 (85.84%), Query Frame = 0

Query: 1   MATNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRKAY 60
           MAT+QPLLQPPKT RSK    ILCFA +LCSA FI N  I  N SE D  FLHN+C KAY
Sbjct: 1   MATHQPLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKSNSSENDLSFLHNLCHKAY 60

Query: 61  NPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDARG 120
           NP+SC+ MAASE PF RE K TN  HFLQ FLRKS+ KV +TI+RA    LS+RIN+ RG
Sbjct: 61  NPSSCIEMAASEFPFPREIKTTNRGHFLQSFLRKSIPKVINTIDRAKD--LSQRINNPRG 120

Query: 121 EAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELEKSL 180
           EAALADC+ELMEIS  RI DS+   AL N+TSG IE+AH WLSSVLTNH+TC DE+E S+
Sbjct: 121 EAALADCVELMEISNSRIIDSV--SALKNRTSGSIENAHTWLSSVLTNHITCWDEVENSV 180

Query: 181 LGTTAAELGLEELIMTARNSLAMLVSISQPEPE--PEIEGQI-GFPGWLKSGDRMLLGVT 240
               A +LGLEELIM  RNSL M VSI + E E   E+E ++ G+P WLK GDR LLGV 
Sbjct: 181 SRAAAMDLGLEELIMRGRNSLGMFVSIWESEIENMGELEKRVNGYPSWLKKGDRRLLGVP 240

Query: 241 GQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMI 300
           G++++ANIVVAKDGSGNF TV EAV SVPDKSKNR VIYVKKG Y+E VEV KKKKNVMI
Sbjct: 241 GKEMEANIVVAKDGSGNFMTVTEAVESVPDKSKNRIVIYVKKGIYKEKVEVGKKKKNVMI 300

Query: 301 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGA 360
           VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTAGPEKHQAVALRVGA
Sbjct: 301 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGA 360

Query: 361 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQ 420
           DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIE RRPMSNQ
Sbjct: 361 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQ 420

Query: 421 ANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIG 480
           ANMVTAQGR+DPNQNTG+SI +C+IVASSDLEPVK+SIKTYLGRPWKEYSRTV+MQS IG
Sbjct: 421 ANMVTAQGRMDPNQNTGSSIQKCEIVASSDLEPVKQSIKTYLGRPWKEYSRTVVMQSTIG 480

Query: 481 DLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSL 540
           DLI PAGWAEWNGDFALKTLYYGEY NNGPGSDVSKRVKW GY VIT+P+EAQKFTVDSL
Sbjct: 481 DLIQPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWAGYHVITSPSEAQKFTVDSL 540

Query: 541 IQGGDWLGPTGVTFIPGL 556
           IQGG+WLGP+G TFIPGL
Sbjct: 541 IQGGEWLGPSGATFIPGL 554

BLAST of MS010790 vs. NCBI nr
Match: XP_004134752.1 (pectinesterase [Cucumis sativus] >KGN49128.1 hypothetical protein Csa_004290 [Cucumis sativus])

HSP 1 Score: 852.8 bits (2202), Expect = 1.6e-243
Identity = 441/561 (78.61%), Postives = 474/561 (84.49%), Query Frame = 0

Query: 1   MATNQP--LLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRK 60
           MAT+QP  LLQPPKT RSK    +LCFA +LCSA FI N  I PNPSE D  FL NIC K
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60

Query: 61  AYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDA 120
           AY+P+SC+ MAASE P     K TN V FLQ FLRKS+ KV  TIERA    + +RIN  
Sbjct: 61  AYDPSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRKSMPKVISTIERAKD--IRQRINSP 120

Query: 121 RGEAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELEK 180
           RGEAALADC+ELMEIS  RI DS+  LAL N+TSG IE++H WLSSVLTNHVTC DE+E 
Sbjct: 121 RGEAALADCIELMEISNGRIMDSV--LALKNRTSGSIENSHTWLSSVLTNHVTCWDEVES 180

Query: 181 SLLGTTAAELGLEELIMTARNSLAMLVSI----SQPEPEPEIEGQIGFPGWLKSGDRMLL 240
           SL      +LGLEELIM  RNSL MLVSI     +   E E +G  G+P WLK GDR LL
Sbjct: 181 SLSRAAPMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGN-GYPSWLKKGDRRLL 240

Query: 241 GVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKN 300
           GV G++++ NIVVAKDGSGNFKTVKEAV SVPDKSKNR VIYVK+GTYEENVEV KKKKN
Sbjct: 241 GVLGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKN 300

Query: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALR 360
           VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTAGPEKHQAVALR
Sbjct: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALR 360

Query: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPM 420
           VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIE RRPM
Sbjct: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM 420

Query: 421 SNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQS 480
           +NQANMVTAQGRIDPNQNTGTSI +C IVASSDLEPVK+SIKTYLGRPWKEYSRTVIMQS
Sbjct: 421 NNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQS 480

Query: 481 RIGDLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTV 540
           RIGDLI PAGWAEWNGDFALKTLYYGEY N GPGSDVSKRVKW GY +IT+P+EAQKFTV
Sbjct: 481 RIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTV 540

Query: 541 DSLIQGGDWLGPTGVTFIPGL 556
           DSLIQGG+WLGP+G TFIPGL
Sbjct: 541 DSLIQGGEWLGPSGATFIPGL 555

BLAST of MS010790 vs. NCBI nr
Match: TYK13062.1 (pectinesterase [Cucumis melo var. makuwa])

HSP 1 Score: 835.9 bits (2158), Expect = 2.1e-238
Identity = 434/561 (77.36%), Postives = 469/561 (83.60%), Query Frame = 0

Query: 1   MATNQPL--LQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRK 60
           MAT+QPL  LQPPKT  SK  S ILCFA +LCSA F+ N  I  NP E D  FLHNIC K
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDA 120
           AYNP+SC+ MAASE P     K TN  HFLQ FL+KS+ KV +TIERA    L +RIN  
Sbjct: 61  AYNPSSCIEMAASEFPL-SIIKTTNEEHFLQSFLKKSMPKVINTIERAKD--LRQRINSP 120

Query: 121 RGEAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELEK 180
           RGE AL DC+ELMEIS  RI DS+  LAL N+TSG IE +H WLSSVLTNHVTC DE+E 
Sbjct: 121 RGEGALDDCIELMEISNGRIMDSV--LALKNRTSGSIESSHTWLSSVLTNHVTCWDEVET 180

Query: 181 SLLGTTAAELGLEELIMTARNSLAMLVSISQPE----PEPEIEGQIGFPGWLKSGDRMLL 240
           SL    A +LGLEELIM  RNSL MLVSI   E     E    G  G+P WLK GDR LL
Sbjct: 181 SLSRAAAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGN-GYPSWLKKGDRRLL 240

Query: 241 GVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKN 300
           GV G++++ NIVVAKDGSGNFKTV+EAV SVPDKSK+R VIYVKKGTY+ENVEV KKKKN
Sbjct: 241 GVLGREMEPNIVVAKDGSGNFKTVQEAVESVPDKSKDRIVIYVKKGTYKENVEVGKKKKN 300

Query: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALR 360
           VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTAGP KHQAVALR
Sbjct: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALR 360

Query: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPM 420
           VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIE RRPM
Sbjct: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM 420

Query: 421 SNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQS 480
           +NQANMVTAQGRIDPNQNTGTSI +C IVASSDLEPVK+SIKTYLGRPWKEYSRTV+MQS
Sbjct: 421 NNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQS 480

Query: 481 RIGDLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTV 540
           RIGDLI PAGWAEW+GDFALKTLYYGEY N GPGSDVSKRVKW GY VIT+P+EAQKFTV
Sbjct: 481 RIGDLIQPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTV 540

Query: 541 DSLIQGGDWLGPTGVTFIPGL 556
           +SLIQGG+WLGP+G +FIPGL
Sbjct: 541 NSLIQGGEWLGPSGASFIPGL 555

BLAST of MS010790 vs. NCBI nr
Match: XP_008439965.1 (PREDICTED: pectinesterase [Cucumis melo])

HSP 1 Score: 835.1 bits (2156), Expect = 3.5e-238
Identity = 434/561 (77.36%), Postives = 468/561 (83.42%), Query Frame = 0

Query: 1   MATNQPL--LQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRK 60
           MAT+QPL  LQPPKT  SK  S ILCFA +LCSA F+ N  I  NP E D  FLHNIC K
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDA 120
           AYNP+SC+ MAASE P     K TN  HFLQ FL+KS+ KV +TIERA    L +RIN  
Sbjct: 61  AYNPSSCIEMAASEFPL-SIIKTTNEEHFLQSFLKKSMPKVINTIERAKD--LRQRINSP 120

Query: 121 RGEAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELEK 180
           RGE AL DC+ELMEIS  RI DS+  LAL N+TSG IE +H WLSSVLTNHVTC DE+E 
Sbjct: 121 RGEGALDDCIELMEISNGRIMDSV--LALKNRTSGYIESSHTWLSSVLTNHVTCWDEVES 180

Query: 181 SLLGTTAAELGLEELIMTARNSLAMLVSISQPE----PEPEIEGQIGFPGWLKSGDRMLL 240
           SL    A +LGLEELIM  RNSL MLVSI   E     E    G  G+P WLK GDR LL
Sbjct: 181 SLSRAAAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGN-GYPSWLKKGDRRLL 240

Query: 241 GVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKN 300
           GV G++++ NIVVAKDGSGNFKTV+EAV SVPDKSKNR VIYVKKGTY+ENVEV KKKKN
Sbjct: 241 GVLGREMEPNIVVAKDGSGNFKTVQEAVESVPDKSKNRIVIYVKKGTYKENVEVGKKKKN 300

Query: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALR 360
           VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTAGP KHQAVALR
Sbjct: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALR 360

Query: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPM 420
           VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIE RRPM
Sbjct: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM 420

Query: 421 SNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQS 480
           +NQANMVTAQGRIDPNQNTGTSI +C IVAS DLEPVK+SIKTYLGRPWKEYSRTV+MQS
Sbjct: 421 NNQANMVTAQGRIDPNQNTGTSIQQCDIVASFDLEPVKKSIKTYLGRPWKEYSRTVVMQS 480

Query: 481 RIGDLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTV 540
           RIGDLI PAGWAEW+GDFALKTLYYGEY N GPGSDVSKRVKW GY VIT+P+EAQKFTV
Sbjct: 481 RIGDLIQPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTV 540

Query: 541 DSLIQGGDWLGPTGVTFIPGL 556
           +SLIQGG+WLGP+G +FIPGL
Sbjct: 541 NSLIQGGEWLGPSGASFIPGL 555

BLAST of MS010790 vs. ExPASy Swiss-Prot
Match: P09607 (Pectinesterase 2.1 OS=Solanum lycopersicum OX=4081 GN=PME2.1 PE=2 SV=2)

HSP 1 Score: 592.0 bits (1525), Expect = 6.8e-168
Identity = 313/561 (55.79%), Postives = 394/561 (70.23%), Query Frame = 0

Query: 1   MAT-NQPLLQPPKTHRSKAFSI--ILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICR 60
           MAT  QPLL   KTH+  +     IL F   L  A F+   L+ P   EI      N+C+
Sbjct: 1   MATPQQPLL--TKTHKQNSIISFKILTFVVTLFVALFLVVFLVAPYQFEIKH---SNLCK 60

Query: 61  KAYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRIND 120
            A +   C++  +  +        ++G+  L+ FL  S+ ++ + I       +  +IND
Sbjct: 61  TAQDSQLCLSYVSDLISNEIVTSDSDGLSILKKFLVYSVHQMNNAIPVVRK--IKNQIND 120

Query: 121 ARGEAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELE 180
            R + AL DCLEL+++S+D + DSI   A++ ++     +A +WLS VLTNHVTC DEL+
Sbjct: 121 IREQGALTDCLELLDLSVDLVCDSI--AAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD 180

Query: 181 ---KSLLGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLL 240
              K+++  T     L+ELI  A+ +LAML S++ P  E    G    P W+ S DR L+
Sbjct: 181 SFTKAMINGT----NLDELISRAKVALAMLASVTTPNDEVLRPGLGKMPSWVSSRDRKLM 240

Query: 241 GVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKN 300
             +G+ I AN VVAKDG+G ++T+ EAVA+ PDKSK R+VIYVK+GTY+ENVEV  +K N
Sbjct: 241 ESSGKDIGANAVVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVSSRKMN 300

Query: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALR 360
           +MI+GDGM +TIITGSLNVVDGSTTF SAT+AAVG GFI QDI  +NTAGP KHQAVALR
Sbjct: 301 LMIIGDGMYATIITGSLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALR 360

Query: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPM 420
           VGAD+SVINRCRIDAYQDTLY HS RQFYRDS +TGT+DFIFGNAAVV Q C++ AR+P 
Sbjct: 361 VGADKSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCQLVARKPG 420

Query: 421 SNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQS 480
             Q NMVTAQGR DPNQ TGTSI  C I+AS DL+PV +   TYLGRPWK+YSRTV+M+S
Sbjct: 421 KYQQNMVTAQGRTDPNQATGTSIQFCDIIASPDLKPVVKEFPTYLGRPWKKYSRTVVMES 480

Query: 481 RIGDLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTV 540
            +G LIDP+GWAEW+GDFALKTLYYGE++NNGPG+  SKRVKWPGY VIT+PAEA  FTV
Sbjct: 481 YLGGLIDPSGWAEWHGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAEAMSFTV 540

Query: 541 DSLIQGGDWLGPTGVTFIPGL 556
             LIQGG WL  T V ++ GL
Sbjct: 541 AKLIQGGSWLRSTDVAYVDGL 548

BLAST of MS010790 vs. ExPASy Swiss-Prot
Match: P83947 (Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=204231 PE=1 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.5e-167
Identity = 319/561 (56.86%), Postives = 391/561 (69.70%), Query Frame = 0

Query: 1   MATNQP-LLQPPKTHRSKA--FSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICR 60
           M  NQP LL+  K+  SK   F +++ FA ++ + F           S  +    H IC 
Sbjct: 1   MEINQPNLLEASKSCYSKITFFLLVISFAALVSTGF-----------SSPELSLHHKICD 60

Query: 61  KAYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRIND 120
           ++ N  SC+AM  SEV       M +  + L+ FL K+  ++    E AN    SRRIN+
Sbjct: 61  QSVNKESCLAM-ISEV---TGLNMADHRNLLKSFLEKTTPRIQKAFETAND--ASRRINN 120

Query: 121 ARGEAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELE 180
            +   AL DC ELM++S +R+ DSI  L   N T+   ED H WLS VLTNHVTC D LE
Sbjct: 121 PQERTALLDCAELMDLSKERVVDSISILFHQNLTTRSHEDLHVWLSGVLTNHVTCLDGLE 180

Query: 181 KSLLG--TTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIG-FPGWLKSGDRMLL 240
           +       T  E  L ELI+ AR SLA+ V++  P     IE   G FP W+ +GDR LL
Sbjct: 181 EGSTDYIKTLMESHLNELILRARTSLAIFVTLF-PAKSNVIEPVTGNFPTWVTAGDRRLL 240

Query: 241 GVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKN 300
              G+ I+ +IVVAKDGSG+++T+ EAVA++PD SK R ++ V+ G YEENV+   +KKN
Sbjct: 241 QTLGKDIEPDIVVAKDGSGDYETLNEAVAAIPDNSKKRVIVLVRTGIYEENVDFGYQKKN 300

Query: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALR 360
           VM+VG+GMD TIITGS NVVDGSTTF SATVAAVGDGFIAQDI F+NTAGPEK+QAVALR
Sbjct: 301 VMLVGEGMDYTIITGSRNVVDGSTTFDSATVAAVGDGFIAQDICFQNTAGPEKYQAVALR 360

Query: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPM 420
           +GAD++VINRCRIDAYQDTLY H+ RQFYRD  ITGTVDFIFGNAAVV QNC +  R+ M
Sbjct: 361 IGADETVINRCRIDAYQDTLYPHNYRQFYRDRNITGTVDFIFGNAAVVFQNCNLIPRKQM 420

Query: 421 SNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQS 480
             Q N +TAQGR DPNQNTGTSI  C+I AS+DLEPV+ + K+YLGRPWKEYSRTV+M+S
Sbjct: 421 KGQENTITAQGRTDPNQNTGTSIQNCEIFASADLEPVEDTFKSYLGRPWKEYSRTVVMES 480

Query: 481 RIGDLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTV 540
            I D+IDPAGW EW+ DFALKTL+YGEY N GPGS  S+RVKWPGY VIT+P  A++FTV
Sbjct: 481 YISDVIDPAGWLEWDRDFALKTLFYGEYRNGGPGSGTSERVKWPGYHVITSPEVAEQFTV 540

Query: 541 DSLIQGGDWLGPTGVTFIPGL 556
             LIQGG WLG TGV +  GL
Sbjct: 541 AELIQGGSWLGSTGVDYTAGL 543

BLAST of MS010790 vs. ExPASy Swiss-Prot
Match: Q96575 (Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 3.4e-167
Identity = 314/561 (55.97%), Postives = 392/561 (69.88%), Query Frame = 0

Query: 1   MAT-NQPLLQPPKTHRSKAFSI--ILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICR 60
           MAT  QPLL   KTH+  +     IL F   L  A F+   L+ P   EI      N+C+
Sbjct: 1   MATPQQPLL--TKTHKQNSIISFKILTFVVTLFVALFLVVFLVAPYQFEIKH---SNLCK 60

Query: 61  KAYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRIND 120
            A +   C++  +  +        ++G+  L  FL   + ++ + I   +   +  +IND
Sbjct: 61  TAQDSQLCLSYVSDLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSK--MKNQIND 120

Query: 121 ARGEAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELE 180
            R E AL DCLEL++ S+D ++DSI   A++ +T     +A +WLS VLTNHVTC DEL+
Sbjct: 121 IRQEGALTDCLELLDQSVDLVSDSI--AAIDKRTHSEHANAQSWLSGVLTNHVTCLDELD 180

Query: 181 ---KSLLGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLL 240
              K+++  T     L+ELI  A+ +LAML S++ P  +    G    P W+ S DR L+
Sbjct: 181 SFTKAMINGT----NLDELISRAKVALAMLASVTTPNDDVLRPGLGKMPSWVSSRDRKLM 240

Query: 241 GVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKN 300
             +G+ I AN VVAKDG+G ++T+ EAVA+ PDKSK R+VIYVK+G Y+ENVEV  +K  
Sbjct: 241 ESSGKDIGANAVVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMK 300

Query: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALR 360
           +MIVGDGM +TIITG+LNVVDGSTTF SAT+AAVG GFI QDI  +NTAGP KHQAVALR
Sbjct: 301 LMIVGDGMHATIITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALR 360

Query: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPM 420
           VGAD+SVINRCRIDAYQDTLY HS RQFYRDS +TGT+DFIFGNAAVV Q CK+ AR+P 
Sbjct: 361 VGADKSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCKLVARKPG 420

Query: 421 SNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQS 480
             Q NMVTAQGR DPNQ TGTSI  C I+ASSDLEPV +   TYLGRPWK+YSRTV+M+S
Sbjct: 421 KYQQNMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMES 480

Query: 481 RIGDLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTV 540
            +G LI+PAGWAEW+GDFALKTLYYGE++NNGPG+  SKRVKWPGY  IT+PAEA  FTV
Sbjct: 481 YLGGLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTV 540

Query: 541 DSLIQGGDWLGPTGVTFIPGL 556
             LIQGG WL  TGV ++ GL
Sbjct: 541 AKLIQGGSWLRSTGVAYVDGL 548

BLAST of MS010790 vs. ExPASy Swiss-Prot
Match: Q1JPL7 (Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PME18 PE=1 SV=3)

HSP 1 Score: 573.5 bits (1477), Expect = 2.5e-162
Identity = 306/554 (55.23%), Postives = 385/554 (69.49%), Query Frame = 0

Query: 3   TNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFL--HNICRKAY 62
           +NQPLL  PK+ + K   ++L F  IL S  F    LI  N +  D   L    IC  A+
Sbjct: 4   SNQPLLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHGAH 63

Query: 63  NPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDARG 122
           +  SC A+  SE      +K+ N +  L VFL+ S+ ++  T+   +   +  R N  R 
Sbjct: 64  DQDSCQAL-LSEFTTLSLSKL-NRLDLLHVFLKNSVWRLESTMTMVSEARI--RSNGVRD 123

Query: 123 EAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDEL-EKS 182
           +A  ADC E+M++S DR+  S+  L   N       + H WLSSVLTN++TC + + + S
Sbjct: 124 KAGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVS 183

Query: 183 LLGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTGQ 242
           +      +  LE+L+  AR +LA+ VS+     + ++     FP WL + DR LL  + +
Sbjct: 184 VNSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMIISNRFPSWLTALDRKLLESSPK 243

Query: 243 QIK--ANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMI 302
            +K  AN+VVAKDG+G FKTV EAVA+ P+ S  R+VIYVKKG Y+E +++ KKKKN+M+
Sbjct: 244 TLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKKNLML 303

Query: 303 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGA 362
           VGDG D+TIITGSLNV+DGSTTF+SATVAA GDGF+AQDIWF+NTAGP KHQAVALRV A
Sbjct: 304 VGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVALRVSA 363

Query: 363 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQ 422
           DQ+VINRCRIDAYQDTLYTH+ RQFYRDS ITGTVDFIFGN+AVV QNC I AR P + Q
Sbjct: 364 DQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNPGAGQ 423

Query: 423 ANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIG 482
            NM+TAQGR D NQNT  SI +C+I ASSDL PVK S+KT+LGRPWK YSRTVIMQS I 
Sbjct: 424 KNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQSFID 483

Query: 483 DLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSL 542
           + IDPAGW  W+G+FAL TLYYGEY N GPG+D SKRV W G++VI +  EA++FTV  L
Sbjct: 484 NHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQFTVAKL 543

Query: 543 IQGGDWLGPTGVTF 552
           IQGG WL PTGVTF
Sbjct: 544 IQGGLWLKPTGVTF 553

BLAST of MS010790 vs. ExPASy Swiss-Prot
Match: P14280 (Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 572.4 bits (1474), Expect = 5.6e-162
Identity = 308/557 (55.30%), Postives = 387/557 (69.48%), Query Frame = 0

Query: 5   QPLLQPPKTHRSK---AFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRKAYN 64
           QPLL   KTH+     +F I+    T+     F+A  L+ P   EI      N+C+ A +
Sbjct: 6   QPLL--IKTHKQNPIISFKILSFVITL-----FVALFLVAPYQVEIKH---SNLCKTAQD 65

Query: 65  PASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDARGE 124
              C++  +  +        ++G   L  FL   + ++ + I       +  +IND R  
Sbjct: 66  SQLCLSYVSDLISNEIVTTESDGHSILMKFLVNYVHQMNNAIPVVRK--MKNQINDIRQH 125

Query: 125 AALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELE---K 184
            AL DCLEL++ S+D  +DSI   A++ ++     +A +WLS VLTNHVTC DEL+   K
Sbjct: 126 GALTDCLELLDQSVDFASDSI--AAIDKRSRSEHANAQSWLSGVLTNHVTCLDELDSFTK 185

Query: 185 SLLGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTG 244
           +++  T     LEELI  A+ +LAML S++  + +  +      P W+ S DR L+  +G
Sbjct: 186 AMINGT----NLEELISRAKVALAMLASLTTQDEDVFMTVLGKMPSWVSSMDRKLMESSG 245

Query: 245 QQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIV 304
           + I AN VVA+DG+G+++T+ EAVA+ PDKSK R+VIYVK+GTY+ENVEV   K N+MIV
Sbjct: 246 KDIIANAVVAQDGTGDYQTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVASNKMNLMIV 305

Query: 305 GDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGAD 364
           GDGM +T ITGSLNVVDGSTTF+SAT+AAVG GFI QDI  +NTAGP K QAVALRVGAD
Sbjct: 306 GDGMYATTITGSLNVVDGSTTFRSATLAAVGQGFILQDICIQNTAGPAKDQAVALRVGAD 365

Query: 365 QSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQA 424
            SVINRCRIDAYQDTLY HS RQFYRDS +TGTVDFIFGNAAVV Q C++ AR+P   Q 
Sbjct: 366 MSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQLVARKPGKYQQ 425

Query: 425 NMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGD 484
           NMVTAQGR DPNQ TGTSI  C I+ASSDLEPV +   TYLGRPWKEYSRTV+M+S +G 
Sbjct: 426 NMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLGG 485

Query: 485 LIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLI 544
           LI+PAGWAEW+GDFALKTLYYGE++NNGPG+  SKRVKWPGY VIT+PA+A  FTV  LI
Sbjct: 486 LINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKLI 544

Query: 545 QGGDWLGPTGVTFIPGL 556
           QGG WL  TGV ++ GL
Sbjct: 546 QGGSWLRSTGVAYVDGL 544

BLAST of MS010790 vs. ExPASy TrEMBL
Match: A0A6J1CN37 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111012508 PE=3 SV=1)

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 552/555 (99.46%), Postives = 552/555 (99.46%), Query Frame = 0

Query: 1   MATNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRKAY 60
           MATNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRKAY
Sbjct: 1   MATNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRKAY 60

Query: 61  NPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDARG 120
           NPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERAN LILSRRINDARG
Sbjct: 61  NPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANDLILSRRINDARG 120

Query: 121 EAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELEKSL 180
           EAALADCLELMEISMDRITDSILALALNNKTS PIEDAHAWLSSVLTNHVTCSDELEKSL
Sbjct: 121 EAALADCLELMEISMDRITDSILALALNNKTSVPIEDAHAWLSSVLTNHVTCSDELEKSL 180

Query: 181 LGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTGQQ 240
           LGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTGQQ
Sbjct: 181 LGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTGQQ 240

Query: 241 IKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIVGD 300
           IKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIVGD
Sbjct: 241 IKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGDLI 480

Query: 481 DPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLIQG 540
           DP GWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLIQG
Sbjct: 481 DPTGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLIQG 540

Query: 541 GDWLGPTGVTFIPGL 556
           GDWLGPTGVTFIPGL
Sbjct: 541 GDWLGPTGVTFIPGL 555

BLAST of MS010790 vs. ExPASy TrEMBL
Match: A0A0A0KHV5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G514890 PE=3 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 7.9e-244
Identity = 441/561 (78.61%), Postives = 474/561 (84.49%), Query Frame = 0

Query: 1   MATNQP--LLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRK 60
           MAT+QP  LLQPPKT RSK    +LCFA +LCSA FI N  I PNPSE D  FL NIC K
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60

Query: 61  AYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDA 120
           AY+P+SC+ MAASE P     K TN V FLQ FLRKS+ KV  TIERA    + +RIN  
Sbjct: 61  AYDPSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRKSMPKVISTIERAKD--IRQRINSP 120

Query: 121 RGEAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELEK 180
           RGEAALADC+ELMEIS  RI DS+  LAL N+TSG IE++H WLSSVLTNHVTC DE+E 
Sbjct: 121 RGEAALADCIELMEISNGRIMDSV--LALKNRTSGSIENSHTWLSSVLTNHVTCWDEVES 180

Query: 181 SLLGTTAAELGLEELIMTARNSLAMLVSI----SQPEPEPEIEGQIGFPGWLKSGDRMLL 240
           SL      +LGLEELIM  RNSL MLVSI     +   E E +G  G+P WLK GDR LL
Sbjct: 181 SLSRAAPMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGN-GYPSWLKKGDRRLL 240

Query: 241 GVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKN 300
           GV G++++ NIVVAKDGSGNFKTVKEAV SVPDKSKNR VIYVK+GTYEENVEV KKKKN
Sbjct: 241 GVLGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKN 300

Query: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALR 360
           VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTAGPEKHQAVALR
Sbjct: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALR 360

Query: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPM 420
           VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIE RRPM
Sbjct: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM 420

Query: 421 SNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQS 480
           +NQANMVTAQGRIDPNQNTGTSI +C IVASSDLEPVK+SIKTYLGRPWKEYSRTVIMQS
Sbjct: 421 NNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQS 480

Query: 481 RIGDLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTV 540
           RIGDLI PAGWAEWNGDFALKTLYYGEY N GPGSDVSKRVKW GY +IT+P+EAQKFTV
Sbjct: 481 RIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTV 540

Query: 541 DSLIQGGDWLGPTGVTFIPGL 556
           DSLIQGG+WLGP+G TFIPGL
Sbjct: 541 DSLIQGGEWLGPSGATFIPGL 555

BLAST of MS010790 vs. ExPASy TrEMBL
Match: A0A5D3CNY5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006640 PE=3 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 1.0e-238
Identity = 434/561 (77.36%), Postives = 469/561 (83.60%), Query Frame = 0

Query: 1   MATNQPL--LQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRK 60
           MAT+QPL  LQPPKT  SK  S ILCFA +LCSA F+ N  I  NP E D  FLHNIC K
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDA 120
           AYNP+SC+ MAASE P     K TN  HFLQ FL+KS+ KV +TIERA    L +RIN  
Sbjct: 61  AYNPSSCIEMAASEFPL-SIIKTTNEEHFLQSFLKKSMPKVINTIERAKD--LRQRINSP 120

Query: 121 RGEAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELEK 180
           RGE AL DC+ELMEIS  RI DS+  LAL N+TSG IE +H WLSSVLTNHVTC DE+E 
Sbjct: 121 RGEGALDDCIELMEISNGRIMDSV--LALKNRTSGSIESSHTWLSSVLTNHVTCWDEVET 180

Query: 181 SLLGTTAAELGLEELIMTARNSLAMLVSISQPE----PEPEIEGQIGFPGWLKSGDRMLL 240
           SL    A +LGLEELIM  RNSL MLVSI   E     E    G  G+P WLK GDR LL
Sbjct: 181 SLSRAAAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGN-GYPSWLKKGDRRLL 240

Query: 241 GVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKN 300
           GV G++++ NIVVAKDGSGNFKTV+EAV SVPDKSK+R VIYVKKGTY+ENVEV KKKKN
Sbjct: 241 GVLGREMEPNIVVAKDGSGNFKTVQEAVESVPDKSKDRIVIYVKKGTYKENVEVGKKKKN 300

Query: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALR 360
           VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTAGP KHQAVALR
Sbjct: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALR 360

Query: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPM 420
           VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIE RRPM
Sbjct: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM 420

Query: 421 SNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQS 480
           +NQANMVTAQGRIDPNQNTGTSI +C IVASSDLEPVK+SIKTYLGRPWKEYSRTV+MQS
Sbjct: 421 NNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQS 480

Query: 481 RIGDLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTV 540
           RIGDLI PAGWAEW+GDFALKTLYYGEY N GPGSDVSKRVKW GY VIT+P+EAQKFTV
Sbjct: 481 RIGDLIQPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTV 540

Query: 541 DSLIQGGDWLGPTGVTFIPGL 556
           +SLIQGG+WLGP+G +FIPGL
Sbjct: 541 NSLIQGGEWLGPSGASFIPGL 555

BLAST of MS010790 vs. ExPASy TrEMBL
Match: A0A1S3B018 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103484595 PE=3 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.7e-238
Identity = 434/561 (77.36%), Postives = 468/561 (83.42%), Query Frame = 0

Query: 1   MATNQPL--LQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRK 60
           MAT+QPL  LQPPKT  SK  S ILCFA +LCSA F+ N  I  NP E D  FLHNIC K
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDA 120
           AYNP+SC+ MAASE P     K TN  HFLQ FL+KS+ KV +TIERA    L +RIN  
Sbjct: 61  AYNPSSCIEMAASEFPL-SIIKTTNEEHFLQSFLKKSMPKVINTIERAKD--LRQRINSP 120

Query: 121 RGEAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELEK 180
           RGE AL DC+ELMEIS  RI DS+  LAL N+TSG IE +H WLSSVLTNHVTC DE+E 
Sbjct: 121 RGEGALDDCIELMEISNGRIMDSV--LALKNRTSGYIESSHTWLSSVLTNHVTCWDEVES 180

Query: 181 SLLGTTAAELGLEELIMTARNSLAMLVSISQPE----PEPEIEGQIGFPGWLKSGDRMLL 240
           SL    A +LGLEELIM  RNSL MLVSI   E     E    G  G+P WLK GDR LL
Sbjct: 181 SLSRAAAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGN-GYPSWLKKGDRRLL 240

Query: 241 GVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKN 300
           GV G++++ NIVVAKDGSGNFKTV+EAV SVPDKSKNR VIYVKKGTY+ENVEV KKKKN
Sbjct: 241 GVLGREMEPNIVVAKDGSGNFKTVQEAVESVPDKSKNRIVIYVKKGTYKENVEVGKKKKN 300

Query: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALR 360
           VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTAGP KHQAVALR
Sbjct: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALR 360

Query: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPM 420
           VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIE RRPM
Sbjct: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM 420

Query: 421 SNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQS 480
           +NQANMVTAQGRIDPNQNTGTSI +C IVAS DLEPVK+SIKTYLGRPWKEYSRTV+MQS
Sbjct: 421 NNQANMVTAQGRIDPNQNTGTSIQQCDIVASFDLEPVKKSIKTYLGRPWKEYSRTVVMQS 480

Query: 481 RIGDLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTV 540
           RIGDLI PAGWAEW+GDFALKTLYYGEY N GPGSDVSKRVKW GY VIT+P+EAQKFTV
Sbjct: 481 RIGDLIQPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTV 540

Query: 541 DSLIQGGDWLGPTGVTFIPGL 556
           +SLIQGG+WLGP+G +FIPGL
Sbjct: 541 NSLIQGGEWLGPSGASFIPGL 555

BLAST of MS010790 vs. ExPASy TrEMBL
Match: A0A6J1KRW2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496691 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 8.7e-235
Identity = 426/557 (76.48%), Postives = 467/557 (83.84%), Query Frame = 0

Query: 1   MATNQPLL-QPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEID-QLFLHNICRK 60
           MAT++PLL QPP+T R K F  IL F+ +L SA FI N LI  NPSE D   FLHNIC K
Sbjct: 1   MATHEPLLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICYK 60

Query: 61  AYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDA 120
           AYN +SC+ M ASE P  +E K TN  +FLQ FLRKS +K+ +TIE A    L RRIN+ 
Sbjct: 61  AYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKD--LRRRINNP 120

Query: 121 RGEAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELEK 180
           RGEAALADC+ELMEISMDRITDSI  LAL  +  G IE+AH WLSSVLTNHV CSDE+E 
Sbjct: 121 RGEAALADCVELMEISMDRITDSI--LALERRRCGTIENAHTWLSSVLTNHVACSDEIEN 180

Query: 181 SLLGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTG 240
           S+    A +LG+EELI+  RNSL MLVSI     +   EG +G+P WLK GDR LL V G
Sbjct: 181 SISKAAAMDLGVEELIVIGRNSLGMLVSIWGSGKK---EG-MGYPSWLKKGDRRLLEVVG 240

Query: 241 QQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIV 300
           + +KA+IVVAKDGSGNFKTVKEAV +VPDKSK+R VIYVKKG Y+ENVEV K KKNVM+V
Sbjct: 241 RGMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLV 300

Query: 301 GDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGAD 360
           GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTA PEKHQAVALRVGAD
Sbjct: 301 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGAD 360

Query: 361 QSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQA 420
           QSVINRCRIDAYQDTLY H+NRQFYRDSTITGTVDFIFGNAAVVLQNCKIE RRPMSNQA
Sbjct: 361 QSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 420

Query: 421 NMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGD 480
           NMVTAQGR DPNQNTGTSI RC+IVAS DLEPVK+SIKTYLGRPWKEYSRTV+MQS +GD
Sbjct: 421 NMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGD 480

Query: 481 LIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLI 540
           LIDPAGWAEWNG+FALKTLYYGEY NNGPGS+VSKRVKWPGY VIT+PA AQKFTVDSLI
Sbjct: 481 LIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLI 540

Query: 541 QGGDWLGPTGVTFIPGL 556
           QG +WLG +GVTFIPGL
Sbjct: 541 QGAEWLGSSGVTFIPGL 549

BLAST of MS010790 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 573.5 bits (1477), Expect = 1.8e-163
Identity = 306/554 (55.23%), Postives = 385/554 (69.49%), Query Frame = 0

Query: 3   TNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFL--HNICRKAY 62
           +NQPLL  PK+ + K   ++L F  IL S  F    LI  N +  D   L    IC  A+
Sbjct: 4   SNQPLLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHGAH 63

Query: 63  NPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDARG 122
           +  SC A+  SE      +K+ N +  L VFL+ S+ ++  T+   +   +  R N  R 
Sbjct: 64  DQDSCQAL-LSEFTTLSLSKL-NRLDLLHVFLKNSVWRLESTMTMVSEARI--RSNGVRD 123

Query: 123 EAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDEL-EKS 182
           +A  ADC E+M++S DR+  S+  L   N       + H WLSSVLTN++TC + + + S
Sbjct: 124 KAGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVS 183

Query: 183 LLGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTGQ 242
           +      +  LE+L+  AR +LA+ VS+     + ++     FP WL + DR LL  + +
Sbjct: 184 VNSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMIISNRFPSWLTALDRKLLESSPK 243

Query: 243 QIK--ANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMI 302
            +K  AN+VVAKDG+G FKTV EAVA+ P+ S  R+VIYVKKG Y+E +++ KKKKN+M+
Sbjct: 244 TLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKKNLML 303

Query: 303 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGA 362
           VGDG D+TIITGSLNV+DGSTTF+SATVAA GDGF+AQDIWF+NTAGP KHQAVALRV A
Sbjct: 304 VGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVALRVSA 363

Query: 363 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQ 422
           DQ+VINRCRIDAYQDTLYTH+ RQFYRDS ITGTVDFIFGN+AVV QNC I AR P + Q
Sbjct: 364 DQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNPGAGQ 423

Query: 423 ANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIG 482
            NM+TAQGR D NQNT  SI +C+I ASSDL PVK S+KT+LGRPWK YSRTVIMQS I 
Sbjct: 424 KNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQSFID 483

Query: 483 DLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSL 542
           + IDPAGW  W+G+FAL TLYYGEY N GPG+D SKRV W G++VI +  EA++FTV  L
Sbjct: 484 NHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQFTVAKL 543

Query: 543 IQGGDWLGPTGVTF 552
           IQGG WL PTGVTF
Sbjct: 544 IQGGLWLKPTGVTF 553

BLAST of MS010790 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 441.4 bits (1134), Expect = 1.1e-123
Identity = 260/546 (47.62%), Postives = 327/546 (59.89%), Query Frame = 0

Query: 52  LHNICRKAYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLIL 111
           L + C     P  C+    S V      ++T+    ++  +  ++T V         LI 
Sbjct: 59  LRSSCSSTRYPELCI----SAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIK 118

Query: 112 SRRINDARGEAALADCLELMEISMDRITDSI--LALALNNKT-SGPIEDAHAWLSSVLTN 171
            R+    R + AL DCLE ++ ++D + +++  L L    KT      D    +SS +TN
Sbjct: 119 KRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITN 178

Query: 172 HVTCSD---------ELEKSLLGTTAAELGLEELIMTARNSLAML-----VSISQPEPEP 231
             TC D         ++ K+LL     ++ +E +     N+LAM+       I+  E + 
Sbjct: 179 QETCLDGFSHDDADKQVRKALL---KGQIHVEHM---CSNALAMIKNMTDTDIANFEQKA 238

Query: 232 EI----------------------EGQI---GFPGWLKSGDRMLLGVTGQQIKANIVVAK 291
           +I                       G++   G+P WL +GDR LL   G  +KA+  VA 
Sbjct: 239 KITSNNRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLL--QGSGVKADATVAA 298

Query: 292 DGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIVGDGMDSTIITG 351
           DGSG FKTV  AVA+ P+ S  R+VI++K G Y ENVEV KKKKN+M +GDG   TIITG
Sbjct: 299 DGSGTFKTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITG 358

Query: 352 SLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGADQSVINRCRIDA 411
           S NVVDGSTTF SATVAAVG+ F+A+DI F+NTAGP KHQAVALRVG+D S    C + A
Sbjct: 359 SRNVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLA 418

Query: 412 YQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQANMVTAQGRIDP 471
           YQDTLY HSNRQF+    I GTVDFIFGNAAVVLQ+C I ARRP S Q NMVTAQGR DP
Sbjct: 419 YQDTLYVHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDP 478

Query: 472 NQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGDLIDPAGWAEWN 531
           NQNTG  I +C+I A+SDL+ VK S  TYLGRPWKEYS+TVIMQS I D+I P GW+EW 
Sbjct: 479 NQNTGIVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWT 538

Query: 532 GDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLIQGGDWLGPTGV 556
           G FAL TL Y EY N G G+  + RVKW G++VIT  AEAQK+T    I GG WL  TG 
Sbjct: 539 GTFALNTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGF 592

BLAST of MS010790 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 438.7 bits (1127), Expect = 6.8e-123
Identity = 255/541 (47.13%), Postives = 319/541 (58.96%), Query Frame = 0

Query: 45  SEIDQLFLHNICRKAYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIE 104
           S      L ++C     P  C +  A+        ++T+    ++  L  +   V     
Sbjct: 55  SSTSHAILKSVCSSTLYPELCFSAVAAT----GGKELTSQKEVIEASLNLTTKAVKHNYF 114

Query: 105 RANHLILSRRINDARGEAALADCLELMEISMDRITDSILALALNNKTSG---PIEDAHAW 164
               LI  R+    R   AL DCLE ++ ++D +  ++  L    K        +D    
Sbjct: 115 AVKKLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTL 174

Query: 165 LSSVLTNHVTCSD---------ELEKSLLGTTAAELGLEELIMTARNSLAMLVSISQPE- 224
           +SS +TN  TC D         ++ K+LL     ++ +E +     N+LAM+ ++++ + 
Sbjct: 175 ISSAITNQGTCLDGFSYDDADRKVRKALL---KGQVHVEHM---CSNALAMIKNMTETDI 234

Query: 225 ------------------PEPEIEGQI---GFPGWLKSGDRMLLGVTGQQIKANIVVAKD 284
                                E+ G +   G+P WL  GDR LL   G  IKA+  VA D
Sbjct: 235 ANFELRDKSSTFTNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLL--QGSTIKADATVADD 294

Query: 285 GSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIVGDGMDSTIITGS 344
           GSG+F TV  AVA+ P+KS  RFVI++K G Y ENVEV KKK N+M +GDG   TIITGS
Sbjct: 295 GSGDFTTVAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGS 354

Query: 345 LNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGADQSVINRCRIDAY 404
            NVVDGSTTF SATVAAVG+ F+A+DI F+NTAGP KHQAVALRVG+D S   +C + AY
Sbjct: 355 RNVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAY 414

Query: 405 QDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQANMVTAQGRIDPN 464
           QDTLY HSNRQF+    ITGTVDFIFGNAA VLQ+C I ARRP S Q NMVTAQGR DPN
Sbjct: 415 QDTLYVHSNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPN 474

Query: 465 QNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGDLIDPAGWAEWNG 524
           QNTG  I  C+I  +SDL  VK +  TYLGRPWKEYSRTVIMQS I D+I P GW EW+G
Sbjct: 475 QNTGIVIQNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSG 534

Query: 525 DFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLIQGGDWLGPTGVT 552
            FAL TL Y EYLN G G+  + RVKW GY+VIT+  EAQ FT    I GG WL  TG  
Sbjct: 535 SFALDTLTYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFP 583

BLAST of MS010790 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 438.7 bits (1127), Expect = 6.8e-123
Identity = 248/576 (43.06%), Postives = 328/576 (56.94%), Query Frame = 0

Query: 2   ATNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIG-----PNPSEIDQLFLHNIC 61
           AT+ P LQ     +   F++ +    ++C   F     +      P  +      +   C
Sbjct: 20  ATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTC 79

Query: 62  RKAYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRIN 121
            K+  P  C+     + P    A     +H   +    +L K +  +  ++ +  ++   
Sbjct: 80  SKSLYPNLCID-TLLDFPGSLTADENELIH---ISFNATLQKFSKALYTSSTITYTQM-- 139

Query: 122 DARGEAALADCLELMEISMDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSD-- 181
             R  +A   CLEL++ S+D +T ++ ++ + +       D   WLSS +TNH TC+D  
Sbjct: 140 PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESH-SDVMTWLSSAMTNHDTCTDGF 199

Query: 182 --------ELEKSLLGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIG----- 241
                   E++  ++G   A   L E++       A  V      P       +G     
Sbjct: 200 DEIEGQGGEVKDQVIG---AVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETE 259

Query: 242 -FPGWLKSGDRMLLGVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKG 301
             P WLK  DR LLG     I+A+I V+KDGSG FKT+ EA+   P+ S  RFVIYVK G
Sbjct: 260 ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAG 319

Query: 302 TY-EENVEVEKKKKNVMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWF 361
            Y EEN++V +KK N+M +GDG   T+ITG  ++ D  TTF +AT AA G GFI +D+ F
Sbjct: 320 RYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTF 379

Query: 362 RNTAGPEKHQAVALRVGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNA 421
            N AGP KHQAVALRVG D +V+ RC I  YQD LY HSNRQF+R+  I GTVDFIFGNA
Sbjct: 380 ENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNA 439

Query: 422 AVVLQNCKIEARRPMSNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYL 481
           AV+LQ+C I AR+PM+ Q   +TAQ R DPNQNTG SIH C+++A+ DLE  K S  TYL
Sbjct: 440 AVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYL 499

Query: 482 GRPWKEYSRTVIMQSRIGDLIDPAGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPG 541
           GRPWK YSR V M S +GD IDP GW EWNG FAL +LYYGEY+N G GS + +RVKWPG
Sbjct: 500 GRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPG 559

Query: 542 YRVITNPAEAQKFTVDSLIQGGDWLGPTGVTFIPGL 556
           Y VIT+  EA KFTV   I G  WL  TGV+F  GL
Sbjct: 560 YHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

BLAST of MS010790 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 434.5 bits (1116), Expect = 1.3e-121
Identity = 248/541 (45.84%), Postives = 330/541 (61.00%), Query Frame = 0

Query: 18  AFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRKAYNPASC---VAMAASEVP 77
           AF   +    ILC    +A+ + G N  ++        C +  NP  C   +   ++  P
Sbjct: 3   AFRAYIINFVILC--ILVASTVSGYNQKDVKAW-----CSQTPNPKPCEYFLTHNSNNEP 62

Query: 78  FHREAKMTNGVHFLQVFLRKSLTKVTDTIERANHLILSRRINDARGEAALADCLELMEIS 137
              E++      FL++ ++  L +    + + +   L  +  D R +AA  DC++L +++
Sbjct: 63  IKSESE------FLKISMKLVLDRA--ILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLT 122

Query: 138 MDRITDSILALALNNKTSGPIEDAHAWLSSVLTNHVTCSDELEKSLLGTTAAELGLEELI 197
           + +I +++      +K      DA  WLS+ LTN  TC        L     ++ L  + 
Sbjct: 123 VSKINETMDPNVKCSKL-----DAQTWLSTALTNLDTC----RAGFLELGVTDIVLPLMS 182

Query: 198 MTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTGQQIKANIVVAKDGSGN 257
               N L   ++I++         + GFP W+K GDR LL       K N VVAKDGSGN
Sbjct: 183 NNVSNLLCNTLAINKVPFNYTPPEKDGFPSWVKPGDRKLL--QSSTPKDNAVVAKDGSGN 242

Query: 258 FKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIVGDGMDSTIITGSLNVV 317
           FKT+KEA+ +       RFVIYVK+G Y EN+E+  +KKNVM+ GDG+  TIITGS +V 
Sbjct: 243 FKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIITGSKSVG 302

Query: 318 DGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGADQSVINRCRIDAYQDTL 377
            G+TTF SATVAAVGDGFIA+ I FRNTAG    QAVALR G+D SV  +C  +AYQDTL
Sbjct: 303 GGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTL 362

Query: 378 YTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQANMVTAQGRIDPNQNTG 437
           Y HSNRQFYRD  + GTVDFIFGNAA VLQNC I ARRP S + N +TAQGR DPNQNTG
Sbjct: 363 YVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRPRS-KTNTITAQGRSDPNQNTG 422

Query: 438 TSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGDLIDPAGWAEWNGDFAL 497
             IH  ++ A+SDL PV  S KTYLGRPW++YSRTV M++ +  LIDP GW EW+G+FAL
Sbjct: 423 IIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFAL 482

Query: 498 KTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLIQGGDWLGPTGVTFIPG 556
           KTL+Y E+ N GPG+  S RV WPG+RV+ + +EA KFTV + + GG W+ P+ V F  G
Sbjct: 483 KTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWI-PSSVPFTSG 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142368.10.0e+0099.46pectinesterase [Momordica charantia][more]
XP_038881670.18.4e-24878.85pectinesterase [Benincasa hispida][more]
XP_004134752.11.6e-24378.61pectinesterase [Cucumis sativus] >KGN49128.1 hypothetical protein Csa_004290 [Cu... [more]
TYK13062.12.1e-23877.36pectinesterase [Cucumis melo var. makuwa][more]
XP_008439965.13.5e-23877.36PREDICTED: pectinesterase [Cucumis melo][more]
Match NameE-valueIdentityDescription
P096076.8e-16855.79Pectinesterase 2.1 OS=Solanum lycopersicum OX=4081 GN=PME2.1 PE=2 SV=2[more]
P839471.5e-16756.86Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=20423... [more]
Q965753.4e-16755.97Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1[more]
Q1JPL72.5e-16255.23Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PM... [more]
P142805.6e-16255.30Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5[more]
Match NameE-valueIdentityDescription
A0A6J1CN370.0e+0099.46Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111012508 PE=3 SV=1[more]
A0A0A0KHV57.9e-24478.61Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G514890 PE=3 SV=1[more]
A0A5D3CNY51.0e-23877.36Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00664... [more]
A0A1S3B0181.7e-23877.36Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103484595 PE=3 SV=1[more]
A0A6J1KRW28.7e-23576.48Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496691 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G11580.11.8e-16355.23methylesterase PCR A [more]
AT3G14310.11.1e-12347.62pectin methylesterase 3 [more]
AT1G53830.16.8e-12347.13pectin methylesterase 2 [more]
AT5G53370.16.8e-12343.06pectin methylesterase PCR fragment F [more]
AT2G45220.11.3e-12145.84Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 47..203
e-value: 1.5E-14
score: 64.4
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 19..179
e-value: 2.6E-16
score: 57.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 51..208
e-value: 3.6E-20
score: 72.8
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 244..540
e-value: 4.6E-154
score: 511.5
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 238..555
e-value: 2.9E-126
score: 423.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 49..208
e-value: 1.2E-23
score: 85.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 51..179
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 19..554
NoneNo IPR availablePANTHERPTHR31707:SF333PECTINESTERASEcoord: 19..554
NoneNo IPR availableCDDcd15799PMEI-like_4coord: 61..208
e-value: 1.86876E-28
score: 108.651
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 388..397
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 239..554

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010790.1MS010790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity