MS010691 (gene) Bitter gourd (TR) v1

Overview


Name	MS010691
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DUF2431 domain-containing protein
Location	scaffold35: 1429611 .. 1430492 (-)
RNA-Seq Expression	MS010691
Synteny	MS010691

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTTAGCGACGGAGAACGAGAGAGAAATGGAATCCTCCGATGAGGAAGAGGAGGAGGAGGAAGAAGAAGAAGTATGGAGGAAACACTACTCATCCAGACACAGAATCTTACTCGTCGGAGAAGGCGATTTCTCCTTCTCTCTGTGTTTGGCCAATCGCTTCGGATCCGCCCGCAACATCGTAGCCACTTCCCTCGATTCTCAGGGTTTGATCCTTGCCTTTTCTTCTGTTCTATGTTGAACTGATTTATCCATTTGATTGCATTTCAGTTGACTGATTTCATTTCATTTGATTTCGCTCCTCGATTTTCGATTTCTCGATTCTCAGATGACCTAGAAAAGAAGTACAGCAACGCAATTCGGAACGTGAGGGAGCTAGAAGAAAGGGGATGTTTGGTATTCTACGGGATCGACGCCCGAAACATGAGCCAACACTTCTTCCTCAGGACGCAGAGATTCGATCGCATCGTCTACAACTTCCCTCACGTCGGATTCCTTTTTCGCGAAGACAGTTTCTGCCAGATCCAGTCAGTAACCGATCGTCTTCTCTTCTTCATTACACATCGAATTTTCCCTTACCACTGGAACTAACTACAGGTTGAACAAGGAATTGGTGGAAGGGTTTCTGAGGAACGGTAAGGCTCTGTTGAAGAAAGGAGATGGGGAGATTCACGTGACGCACAAGGAAGGGGAACCGTACGACAAGTGGGAGCTGGTGGAGGAAGCCATGAAAATGGGGCTCAAGTTGCAGGAAGCGGTGCCGTTCCGGAGGGGTGATTATCCGGGATATGAGAACAAGAGAGCCGATGGTGGTCAATCGGATGAGCCATTTAGGCTTGGGGATTGCAGCACTTACAAGTTTAAGCTCGATGAATCTCGC

mRNA sequence

ATGAAGTTAGCGACGGAGAACGAGAGAGAAATGGAATCCTCCGATGAGGAAGAGGAGGAGGAGGAAGAAGAAGAAGTATGGAGGAAACACTACTCATCCAGACACAGAATCTTACTCGTCGGAGAAGGCGATTTCTCCTTCTCTCTGTGTTTGGCCAATCGCTTCGGATCCGCCCGCAACATCGTAGCCACTTCCCTCGATTCTCAGGATGACCTAGAAAAGAAGTACAGCAACGCAATTCGGAACGTGAGGGAGCTAGAAGAAAGGGGATGTTTGGTATTCTACGGGATCGACGCCCGAAACATGAGCCAACACTTCTTCCTCAGGACGCAGAGATTCGATCGCATCGTCTACAACTTCCCTCACGTCGGATTCCTTTTTCGCGAAGACAGTTTCTGCCAGATCCAGTTGAACAAGGAATTGGTGGAAGGGTTTCTGAGGAACGGTAAGGCTCTGTTGAAGAAAGGAGATGGGGAGATTCACGTGACGCACAAGGAAGGGGAACCGTACGACAAGTGGGAGCTGGTGGAGGAAGCCATGAAAATGGGGCTCAAGTTGCAGGAAGCGGTGCCGTTCCGGAGGGGTGATTATCCGGGATATGAGAACAAGAGAGCCGATGGTGGTCAATCGGATGAGCCATTTAGGCTTGGGGATTGCAGCACTTACAAGTTTAAGCTCGATGAATCTCGC

Coding sequence (CDS)

ATGAAGTTAGCGACGGAGAACGAGAGAGAAATGGAATCCTCCGATGAGGAAGAGGAGGAGGAGGAAGAAGAAGAAGTATGGAGGAAACACTACTCATCCAGACACAGAATCTTACTCGTCGGAGAAGGCGATTTCTCCTTCTCTCTGTGTTTGGCCAATCGCTTCGGATCCGCCCGCAACATCGTAGCCACTTCCCTCGATTCTCAGGATGACCTAGAAAAGAAGTACAGCAACGCAATTCGGAACGTGAGGGAGCTAGAAGAAAGGGGATGTTTGGTATTCTACGGGATCGACGCCCGAAACATGAGCCAACACTTCTTCCTCAGGACGCAGAGATTCGATCGCATCGTCTACAACTTCCCTCACGTCGGATTCCTTTTTCGCGAAGACAGTTTCTGCCAGATCCAGTTGAACAAGGAATTGGTGGAAGGGTTTCTGAGGAACGGTAAGGCTCTGTTGAAGAAAGGAGATGGGGAGATTCACGTGACGCACAAGGAAGGGGAACCGTACGACAAGTGGGAGCTGGTGGAGGAAGCCATGAAAATGGGGCTCAAGTTGCAGGAAGCGGTGCCGTTCCGGAGGGGTGATTATCCGGGATATGAGAACAAGAGAGCCGATGGTGGTCAATCGGATGAGCCATTTAGGCTTGGGGATTGCAGCACTTACAAGTTTAAGCTCGATGAATCTCGC

Protein sequence

MKLATENEREMESSDEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLKLQEAVPFRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFKLDESR

Homology

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BLAST of MS010691 vs. NCBI nr
Match: XP_022132615.1 (uncharacterized protein At4g26485-like [Momordica charantia])

HSP 1 Score: 456.8 bits (1174), Expect = 1.1e-124
Identity = 229/232 (98.71%), Postives = 230/232 (99.14%), Query Frame = 0


Query: 1   MKLATENEREMESSD--EEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSA 60
           MKLATENEREMESSD  EEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSA
Sbjct: 1   MKLATENEREMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSA 60

Query: 61  RNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVY 120
           RNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVY
Sbjct: 61  RNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVY 120

Query: 121 NFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEE 180
           NFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEE
Sbjct: 121 NFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEE 180

Query: 181 AMKMGLKLQEAVPFRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFKLDESR 231
           AMKMGLKLQEAVPFRRGDYPGY+NKRADGGQSDEPFRLGDCSTYKFKLDESR
Sbjct: 181 AMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDESR 232

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BLAST of MS010691 vs. NCBI nr
Match: XP_023003736.1 (uncharacterized protein At4g26485-like [Cucurbita maxima])

HSP 1 Score: 388.7 bits (997), Expect = 3.6e-104
Identity = 191/224 (85.27%), Postives = 206/224 (91.96%), Query Frame = 0


Query: 6   ENEREMESSDEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATS 65
           ENE +MESSD    E+EEEEVWRKHYSS+HRILLVGEGDFSFSLCLAN FGSARNIVAT 
Sbjct: 5   ENEGKMESSD----EDEEEEVWRKHYSSKHRILLVGEGDFSFSLCLANHFGSARNIVATC 64

Query: 66  LDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVGF 125
            DSQDDLE+K+S+ IRNVRELEERGCLVFYGID RNMSQHFFLRTQRFDRIVYNFPHVGF
Sbjct: 65  RDSQDDLERKFSDGIRNVRELEERGCLVFYGIDVRNMSQHFFLRTQRFDRIVYNFPHVGF 124

Query: 126 LFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLK 185
           LFREDSFCQIQLNKELVEGFLRN + LLKKG+GEIHVTHKEGEPY+KWELVEEAMK GL+
Sbjct: 125 LFREDSFCQIQLNKELVEGFLRNARVLLKKGEGEIHVTHKEGEPYNKWELVEEAMKAGLQ 184

Query: 186 LQEAVPFRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFKLDES 230
           LQE VPF + DYPGY+NKRADGGQS+ PF LGDCSTYKFKL++S
Sbjct: 185 LQETVPFCKNDYPGYQNKRADGGQSNAPFWLGDCSTYKFKLNQS 224

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BLAST of MS010691 vs. NCBI nr
Match: XP_022926856.1 (uncharacterized protein At4g26485-like [Cucurbita moschata])

HSP 1 Score: 388.3 bits (996), Expect = 4.7e-104
Identity = 190/224 (84.82%), Postives = 207/224 (92.41%), Query Frame = 0


Query: 6   ENEREMESSDEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATS 65
           ENE +MESSD   E++EE+EVWRKHYSS+HRILLVGEGDFSF+LCLAN FGSARNIVAT 
Sbjct: 5   ENEVKMESSD---EDDEEDEVWRKHYSSKHRILLVGEGDFSFALCLANHFGSARNIVATC 64

Query: 66  LDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVGF 125
           LDSQDDLE+K+S+ IRNVRELEERGCLVFYGID RNMSQHFFLRTQRFDRIVYNFPHVGF
Sbjct: 65  LDSQDDLERKFSDGIRNVRELEERGCLVFYGIDVRNMSQHFFLRTQRFDRIVYNFPHVGF 124

Query: 126 LFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLK 185
           LFREDSFCQIQLNKELVEGFLRN + LLKKGDGEIHVTHKEGEPY+KWELVEEAMK GL+
Sbjct: 125 LFREDSFCQIQLNKELVEGFLRNARVLLKKGDGEIHVTHKEGEPYNKWELVEEAMKAGLQ 184

Query: 186 LQEAVPFRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFKLDES 230
           LQE VPF + DYPGY+NKRADGG S+ PF LGDCSTYKFKL++S
Sbjct: 185 LQETVPFCKNDYPGYQNKRADGGHSNAPFWLGDCSTYKFKLNQS 225

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BLAST of MS010691 vs. NCBI nr
Match: XP_011658099.1 (uncharacterized protein At4g26485 [Cucumis sativus] >KGN49019.1 hypothetical protein Csa_003253 [Cucumis sativus])

HSP 1 Score: 387.5 bits (994), Expect = 8.0e-104
Identity = 191/230 (83.04%), Postives = 208/230 (90.43%), Query Frame = 0


Query: 1   MKLATENEREMESSDE-EEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSAR 60
           M+L  ENER  ESSDE E+E+EEEEE+WRKHYSSRHRILLVGEGDFSF+LCLA  FG A 
Sbjct: 1   MELLPENERRTESSDEDEDEDEEEEEIWRKHYSSRHRILLVGEGDFSFALCLAKHFGCAC 60

Query: 61  NIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYN 120
           NIVAT LDSQDDLEKKYS+ IRNVRELEERGCL+FYGID RNMS HFFLRTQRFDRIVYN
Sbjct: 61  NIVATCLDSQDDLEKKYSDGIRNVRELEERGCLIFYGIDVRNMSTHFFLRTQRFDRIVYN 120

Query: 121 FPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEA 180
           FPHVGFL+REDSFCQIQLNKELVEGFLRN + LLKK DGEIHV+HKEGEPY+KWELVEEA
Sbjct: 121 FPHVGFLYREDSFCQIQLNKELVEGFLRNARVLLKKLDGEIHVSHKEGEPYNKWELVEEA 180

Query: 181 MKMGLKLQEAVPFRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFKLDES 230
           MK+GLKL+E VPF + DYPGYENKRADGG S+ PF LGDCSTYKFKL++S
Sbjct: 181 MKVGLKLEETVPFNKKDYPGYENKRADGGHSNAPFWLGDCSTYKFKLNQS 230

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BLAST of MS010691 vs. NCBI nr
Match: XP_038881536.1 (uncharacterized protein At4g26485-like [Benincasa hispida])

HSP 1 Score: 387.5 bits (994), Expect = 8.0e-104
Identity = 190/229 (82.97%), Postives = 206/229 (89.96%), Query Frame = 0


Query: 1   MKLATENEREMESSDEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARN 60
           M+L  ENER  ESSD  EE +EEEEVWRKHYSSRHRILLVGEGDFSF+LCLA  FGSARN
Sbjct: 1   MELLPENERRTESSD--EENDEEEEVWRKHYSSRHRILLVGEGDFSFALCLAKHFGSARN 60

Query: 61  IVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNF 120
           IVAT LDSQDDLEKKYS+ +RNVRELEERGCLVFYGID RNMS HFFLRTQRFDRI+YNF
Sbjct: 61  IVATCLDSQDDLEKKYSDGLRNVRELEERGCLVFYGIDVRNMSTHFFLRTQRFDRIIYNF 120

Query: 121 PHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAM 180
           PHVGFLFREDSFCQIQLNKELVEGFL+N + LLKK DGEIHV+HKEGEPY+KWELVEEAM
Sbjct: 121 PHVGFLFREDSFCQIQLNKELVEGFLKNARVLLKKVDGEIHVSHKEGEPYNKWELVEEAM 180

Query: 181 KMGLKLQEAVPFRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFKLDES 230
           K+GLKLQE VPF + DYPGY+NKRADGG S+ PF LGDC TYKFKL++S
Sbjct: 181 KLGLKLQETVPFYKNDYPGYQNKRADGGHSNAPFWLGDCCTYKFKLNQS 227

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BLAST of MS010691 vs. ExPASy Swiss-Prot
Match: F4I1X0 (Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX=3702 GN=HIPP41 PE=3 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 1.1e-52
Identity = 107/214 (50.00%), Postives = 144/214 (67.29%), Query Frame = 0


Query: 12  ESSDEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDD 71
           E S    + + +EEVW KHYSS H+ILLVGEGDFSFS  LA  FGSA NI A+SLDS D 
Sbjct: 5   ELSQLNRDSDGDEEVWVKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDV 64

Query: 72  LEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVGFLFREDS 131
           + +KY  A  N++ L+  G L+ +G+DA  +  H  LR +RFDR+++NFPH GF  RE  
Sbjct: 65  VVRKYKKARSNLKTLKRLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESD 124

Query: 132 FCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLKLQEAVP 191
              I+ ++ELV GF  NG + L + +GE+HV+HK   P+ +W L E A +  L L + V 
Sbjct: 125 SSLIRKHRELVFGFF-NGASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVA 184

Query: 192 FRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFK 226
           F + +YPGYENKR DG + D+PF LG+CST+KF+
Sbjct: 185 FEKNNYPGYENKRGDGRRCDQPFLLGECSTFKFR 217

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BLAST of MS010691 vs. ExPASy Swiss-Prot
Match: P0C8L4 (Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 PE=4 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.5e-49
Identity = 106/206 (51.46%), Postives = 135/206 (65.53%), Query Frame = 0


Query: 22  EEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKYSNAIR 81
           E  E W +HYSS H+ILLVGEG+FSFSLCLA+ FGSA NI ATSLDS+D+L  KY +A+ 
Sbjct: 2   EISEKWIRHYSSTHKILLVGEGNFSFSLCLASAFGSAMNITATSLDSEDELSIKYMDAVD 61

Query: 82  NVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVG--FLFREDSFCQIQLNK 141
           N+  L+  GC + + +D   MS    L  QR+DRIV+NFPH G  F  RE S   I+ +K
Sbjct: 62  NINILKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFNFPHAGSRFFGRELSSRAIESHK 121

Query: 142 ELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLKLQEAVPFRRGDYPG 201
           ELV GFL N K +L++ DGEIH+THK   P+  W + +     GLKL +   F    YPG
Sbjct: 122 ELVRGFLENAKEMLEE-DGEIHITHKTTYPFSDWGIKKLGKGEGLKLLKKSKFELSHYPG 181

Query: 202 YENKRADGG-QSDEPFRLGDCSTYKF 225
           Y  KR  GG +SD+ F +G+CSTY F
Sbjct: 182 YITKRGSGGRRSDDYFPVGECSTYMF 206

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BLAST of MS010691 vs. ExPASy Swiss-Prot
Match: O94480 (25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bmt5 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.3e-19
Identity = 67/201 (33.33%), Postives = 104/201 (51.74%), Query Frame = 0


Query: 31  YSSRHRILLVGEGDFSFSL-CLANRFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEER 90
           +   +R LL+GEG+FSF+   L +   S   ++ATS DS++DL++KY +A   + ++E  
Sbjct: 54  FEKNNRFLLLGEGNFSFAFSLLLHHVSSEGFVLATSYDSKEDLKQKYPDAAEYISKIEIN 113

Query: 91  GCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVGFLFREDSFCQIQLNKELVEGFLRNG 150
           G  V + IDA  +  H  L+TQ+FD I +NFPH G   + D    I  N++++  F +  
Sbjct: 114 GGKVMHEIDATKLHLHKKLKTQKFDTIFWNFPHSGKGIK-DQDRNILDNQKMLLAFFKAS 173

Query: 151 KALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLKLQEAVPFRRGDYPGYENKRADGGQ 210
           K LL +  G I +T  E +PY  W L   A   G        F    YP Y ++R  G  
Sbjct: 174 KFLLSE-KGVIVITLAETKPYTLWNLKGLAKDAGYTSLMTEKFDSSFYPEYSHRRTIGWI 233

Query: 211 SDEPFRLGDCSTYKFKLDESR 231
                 + + S +K +L +SR
Sbjct: 234 DG----ISERSPWKGELRDSR 248

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BLAST of MS010691 vs. ExPASy Swiss-Prot
Match: Q3UY23 (Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus musculus OX=10090 GN=Fdxacb1 PE=2 SV=2)

HSP 1 Score: 89.4 bits (220), Expect = 5.9e-17
Identity = 66/200 (33.00%), Postives = 94/200 (47.00%), Query Frame = 0


Query: 36  RILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFY 95
           R+LLVGEG+FSF+  L +    + ++ AT    +  LE     A+ N++ L ERG  V +
Sbjct: 5   RLLLVGEGNFSFAASLIDGLDPSVSVTATGFQHRAALEGD-PVALENLKRLRERGVEVRF 64

Query: 96  GIDARNMSQHFFLRTQRFDRIVYNFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKK 155
           G+D   +S       + FDRI +NFPH G          +  N+EL+  F ++   +L K
Sbjct: 65  GVDCTQLSHALPADDRDFDRIYFNFPHCG------RKAGVAKNRELLAKFFQSCADVLAK 124

Query: 156 GDGEIHVT---HKEGEPYDK--------WELVEEAMKMGLKLQEAVPFRRGDYPGYENKR 215
             GE+HVT    + G P DK        W++V  A   G  L +  PF     PGY  K 
Sbjct: 125 A-GEVHVTLCRGQGGTPADKPQREWHNSWQVVAMAALGGFILSDVCPFSCEAVPGY--KC 184

Query: 216 ADGGQSDEPFRLGDCSTYKF 225
                 D PF +    TY F
Sbjct: 185 TGYRSQDRPFHIEGALTYIF 194

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BLAST of MS010691 vs. ExPASy Swiss-Prot
Match: Q9BRP7 (Ferredoxin-fold anticodon-binding domain-containing protein 1 OS=Homo sapiens OX=9606 GN=FDXACB1 PE=1 SV=3)

HSP 1 Score: 77.8 bits (190), Expect = 1.8e-13
Identity = 56/177 (31.64%), Postives = 87/177 (49.15%), Query Frame = 0


Query: 36  RILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFY 95
           R+LLVGEG+FSF+  L+     +  + AT L    +L +    A  N++ L ERG  V +
Sbjct: 5   RLLLVGEGNFSFAAALSETLDQSTQLTATCLQRPAELARD-PLAWENLQCLRERGIDVRF 64

Query: 96  GIDARNMSQHFFLRTQRFDRIVYNFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKK 155
           G+D   ++  F L  + FD+I + FPH G          +  N+EL+  F ++   +L +
Sbjct: 65  GVDCTQLADVFELHEREFDQIYFIFPHCG------RKAGVAKNRELLAKFFQSCADVLAE 124

Query: 156 GDGEIHVT---HKEGEPYDK--------WELVEEAMKMGLKLQEAVPFRRGDYPGYE 202
            +GE+HV     + G P DK        W++V  A   GL L +  PF      GY+
Sbjct: 125 -EGEVHVALCRGQGGTPADKPQREWHNSWQVVAMAALGGLILSDVYPFSCKAVAGYK 173

[Close]

BLAST of MS010691 vs. ExPASy TrEMBL
Match: A0A6J1BSY8 (uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC111005434 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 5.2e-125
Identity = 229/232 (98.71%), Postives = 230/232 (99.14%), Query Frame = 0


Query: 1   MKLATENEREMESSD--EEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSA 60
           MKLATENEREMESSD  EEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSA
Sbjct: 1   MKLATENEREMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSA 60

Query: 61  RNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVY 120
           RNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVY
Sbjct: 61  RNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVY 120

Query: 121 NFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEE 180
           NFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEE
Sbjct: 121 NFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEE 180

Query: 181 AMKMGLKLQEAVPFRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFKLDESR 231
           AMKMGLKLQEAVPFRRGDYPGY+NKRADGGQSDEPFRLGDCSTYKFKLDESR
Sbjct: 181 AMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDESR 232

[Close]

BLAST of MS010691 vs. ExPASy TrEMBL
Match: A0A6J1KQ33 (uncharacterized protein At4g26485-like OS=Cucurbita maxima OX=3661 GN=LOC111497233 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.7e-104
Identity = 191/224 (85.27%), Postives = 206/224 (91.96%), Query Frame = 0


Query: 6   ENEREMESSDEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATS 65
           ENE +MESSD    E+EEEEVWRKHYSS+HRILLVGEGDFSFSLCLAN FGSARNIVAT 
Sbjct: 5   ENEGKMESSD----EDEEEEVWRKHYSSKHRILLVGEGDFSFSLCLANHFGSARNIVATC 64

Query: 66  LDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVGF 125
            DSQDDLE+K+S+ IRNVRELEERGCLVFYGID RNMSQHFFLRTQRFDRIVYNFPHVGF
Sbjct: 65  RDSQDDLERKFSDGIRNVRELEERGCLVFYGIDVRNMSQHFFLRTQRFDRIVYNFPHVGF 124

Query: 126 LFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLK 185
           LFREDSFCQIQLNKELVEGFLRN + LLKKG+GEIHVTHKEGEPY+KWELVEEAMK GL+
Sbjct: 125 LFREDSFCQIQLNKELVEGFLRNARVLLKKGEGEIHVTHKEGEPYNKWELVEEAMKAGLQ 184

Query: 186 LQEAVPFRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFKLDES 230
           LQE VPF + DYPGY+NKRADGGQS+ PF LGDCSTYKFKL++S
Sbjct: 185 LQETVPFCKNDYPGYQNKRADGGQSNAPFWLGDCSTYKFKLNQS 224

[Close]

BLAST of MS010691 vs. ExPASy TrEMBL
Match: A0A6J1EG15 (uncharacterized protein At4g26485-like OS=Cucurbita moschata OX=3662 GN=LOC111433842 PE=4 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.3e-104
Identity = 190/224 (84.82%), Postives = 207/224 (92.41%), Query Frame = 0


Query: 6   ENEREMESSDEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATS 65
           ENE +MESSD   E++EE+EVWRKHYSS+HRILLVGEGDFSF+LCLAN FGSARNIVAT 
Sbjct: 5   ENEVKMESSD---EDDEEDEVWRKHYSSKHRILLVGEGDFSFALCLANHFGSARNIVATC 64

Query: 66  LDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVGF 125
           LDSQDDLE+K+S+ IRNVRELEERGCLVFYGID RNMSQHFFLRTQRFDRIVYNFPHVGF
Sbjct: 65  LDSQDDLERKFSDGIRNVRELEERGCLVFYGIDVRNMSQHFFLRTQRFDRIVYNFPHVGF 124

Query: 126 LFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLK 185
           LFREDSFCQIQLNKELVEGFLRN + LLKKGDGEIHVTHKEGEPY+KWELVEEAMK GL+
Sbjct: 125 LFREDSFCQIQLNKELVEGFLRNARVLLKKGDGEIHVTHKEGEPYNKWELVEEAMKAGLQ 184

Query: 186 LQEAVPFRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFKLDES 230
           LQE VPF + DYPGY+NKRADGG S+ PF LGDCSTYKFKL++S
Sbjct: 185 LQETVPFCKNDYPGYQNKRADGGHSNAPFWLGDCSTYKFKLNQS 225

[Close]

BLAST of MS010691 vs. ExPASy TrEMBL
Match: A0A0A0KJQ9 (DUF2431 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G510860 PE=4 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 3.9e-104
Identity = 191/230 (83.04%), Postives = 208/230 (90.43%), Query Frame = 0


Query: 1   MKLATENEREMESSDE-EEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSAR 60
           M+L  ENER  ESSDE E+E+EEEEE+WRKHYSSRHRILLVGEGDFSF+LCLA  FG A 
Sbjct: 1   MELLPENERRTESSDEDEDEDEEEEEIWRKHYSSRHRILLVGEGDFSFALCLAKHFGCAC 60

Query: 61  NIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYN 120
           NIVAT LDSQDDLEKKYS+ IRNVRELEERGCL+FYGID RNMS HFFLRTQRFDRIVYN
Sbjct: 61  NIVATCLDSQDDLEKKYSDGIRNVRELEERGCLIFYGIDVRNMSTHFFLRTQRFDRIVYN 120

Query: 121 FPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEA 180
           FPHVGFL+REDSFCQIQLNKELVEGFLRN + LLKK DGEIHV+HKEGEPY+KWELVEEA
Sbjct: 121 FPHVGFLYREDSFCQIQLNKELVEGFLRNARVLLKKLDGEIHVSHKEGEPYNKWELVEEA 180

Query: 181 MKMGLKLQEAVPFRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFKLDES 230
           MK+GLKL+E VPF + DYPGYENKRADGG S+ PF LGDCSTYKFKL++S
Sbjct: 181 MKVGLKLEETVPFNKKDYPGYENKRADGGHSNAPFWLGDCSTYKFKLNQS 230

[Close]

BLAST of MS010691 vs. ExPASy TrEMBL
Match: A0A5D3CMV7 (DUF2431 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00400 PE=4 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 5.1e-104
Identity = 192/236 (81.36%), Postives = 207/236 (87.71%), Query Frame = 0


Query: 1   MKLATENER-------EMESSDEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLAN 60
           M+   ENER       E E  +EEEEEEEEEE+WRKHYSSRHRILLVGEGDFSF+LCLA 
Sbjct: 1   MEFVPENERTESSDEDEDEEEEEEEEEEEEEEIWRKHYSSRHRILLVGEGDFSFALCLAK 60

Query: 61  RFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRF 120
            FGSARNIVAT LDSQDDLEKKYS+ IRNVRELEERGCL+FYGID RNMS HFFLRTQRF
Sbjct: 61  HFGSARNIVATCLDSQDDLEKKYSDGIRNVRELEERGCLIFYGIDVRNMSTHFFLRTQRF 120

Query: 121 DRIVYNFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKW 180
           DRIVYNFPHVGFL+REDSFCQIQLNKELVEGFLRN K LLKK DGEIHV+HKEGEPY+KW
Sbjct: 121 DRIVYNFPHVGFLYREDSFCQIQLNKELVEGFLRNAKVLLKKLDGEIHVSHKEGEPYNKW 180

Query: 181 ELVEEAMKMGLKLQEAVPFRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFKLDES 230
           ELVEEAMK+GLKL+E VPF + DYPGY+NKRADGG S+ PF LGDCSTYKFKL+ S
Sbjct: 181 ELVEEAMKVGLKLEETVPFHKKDYPGYQNKRADGGHSNAPFWLGDCSTYKFKLNHS 236

[Close]

BLAST of MS010691 vs. TAIR 10
Match: AT1G55790.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 208.0 bits (528), Expect = 8.1e-54
Identity = 107/214 (50.00%), Postives = 144/214 (67.29%), Query Frame = 0


Query: 12  ESSDEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDD 71
           E S    + + +EEVW KHYSS H+ILLVGEGDFSFS  LA  FGSA NI A+SLDS D 
Sbjct: 5   ELSQLNRDSDGDEEVWVKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDV 64

Query: 72  LEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVGFLFREDS 131
           + +KY  A  N++ L+  G L+ +G+DA  +  H  LR +RFDR+++NFPH GF  RE  
Sbjct: 65  VVRKYKKARSNLKTLKRLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESD 124

Query: 132 FCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLKLQEAVP 191
              I+ ++ELV GF  NG + L + +GE+HV+HK   P+ +W L E A +  L L + V 
Sbjct: 125 SSLIRKHRELVFGFF-NGASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVA 184

Query: 192 FRRGDYPGYENKRADGGQSDEPFRLGDCSTYKFK 226
           F + +YPGYENKR DG + D+PF LG+CST+KF+
Sbjct: 185 FEKNNYPGYENKRGDGRRCDQPFLLGECSTFKFR 217

[Close]

BLAST of MS010691 vs. TAIR 10
Match: AT5G56060.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 165.6 bits (418), Expect = 4.6e-41
Identity = 86/189 (45.50%), Postives = 121/189 (64.02%), Query Frame = 0


Query: 29  KHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEE 88
           + YS++ +ILLVGEGDFSFSL LA  FGSA NI ATSLD++++L  KY++   NV  LE 
Sbjct: 10  QQYSNKQKILLVGEGDFSFSLSLARVFGSATNITATSLDTREELGIKYTDGKANVEGLEL 69

Query: 89  RGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVGFLF-REDSFCQIQLNKELVEGFLR 148
            GC V +G++  +MS  +  R  R+DRI++NFPH G  F  E     I L++ LV GFL 
Sbjct: 70  FGCTVVHGVNVHSMSSDY--RLGRYDRIIFNFPHSGLGFGSEHDIFFIMLHQGLVRGFLE 129

Query: 149 NGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLKLQEAVPFRRGDYPGYENKRADG 208
           + + +LK  DGEIHVTHK  +P+++W +   A + GL+L   + F +  +PGY NK+  G
Sbjct: 130 SARKMLKDEDGEIHVTHKTTDPFNRWGIETLAGEKGLRLIGEIEFHKWAFPGYSNKKGGG 189

Query: 209 GQSDEPFRL 217
              +  F L
Sbjct: 190 SNCNSTFLL 196

[Close]

BLAST of MS010691 vs. TAIR 10
Match: AT5G56075.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 156.8 bits (395), Expect = 2.2e-38
Identity = 90/211 (42.65%), Postives = 122/211 (57.82%), Query Frame = 0


Query: 17  EEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKY 76
           E +   +E    +HY+++ +ILLVGEGDFSFSL LA  FGSA N+ ATSLD+Q +LE+K+
Sbjct: 56  EMQTNVQETKRLRHYTNKQKILLVGEGDFSFSLSLARAFGSASNLTATSLDTQGELEQKF 115

Query: 77  SNAIRNVRELEERGCLVFYGIDARNM-SQHFFLRTQRFDRIVYNFPHVGFLFREDSFCQI 136
            N   NV ELE  GC V YG++  +M ++     +  +DR+++NFP              
Sbjct: 116 KNGKANVEELERLGCSVVYGVNVHSMITKPSVGGSAIYDRVIFNFP-------------- 175

Query: 137 QLNKELVEGFLRNGKALLKKGD--GEIHVTHKEGEPYDKWELVEEAMKMGLKLQEAVPFR 196
               ELV GF+++ + L+K  D  GEIHV HK   P+ +W+L     K GL L   V F 
Sbjct: 176 --THELVRGFMKSARVLVKDEDKGGEIHVIHKTEYPFSEWKLKTLGEKEGLDLIREVEFC 235

Query: 197 RGDYPGYENKRADGGQSDEPFRLGDCSTYKF 225
              YPGY NKR  GG SD  F +G  ST+ F
Sbjct: 236 LSHYPGYFNKRGSGGYSDSSFPVGKSSTFMF 250

[Close]

BLAST of MS010691 vs. TAIR 10
Match: AT1G55800.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 155.2 bits (391), Expect = 6.3e-38
Identity = 95/245 (38.78%), Postives = 129/245 (52.65%), Query Frame = 0


Query: 12  ESSDEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDS--- 71
           E S+   + + +EEVW KHYSS+H+ILLVGEGDFSFS  LA  FGSA NI A+SLDS   
Sbjct: 3   ELSELNRDRDGDEEVWVKHYSSKHQILLVGEGDFSFSCSLATCFGSASNIYASSLDSYDY 62

Query: 72  ------------------------QDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQ 131
                                    DD+ +KY NA  N+  L+  G  + +G+DA  +  
Sbjct: 63  KPVNKGCSFKLDFLSCCMSFMVIKPDDVVRKYKNARSNLETLKRLGAFLLHGVDATTLHF 122

Query: 132 HFFLRTQRFDRIVYNFPHVGFLFREDSFCQIQ----LNKELVEGFLRNGKALLKKGDGEI 191
           H  LR +RFDR+++NFPH GF  +E   CQIQ      + L + FL +G + + + DGE+
Sbjct: 123 HPDLRYRRFDRVIFNFPHTGFHRKESDPCQIQPAAATLRNLFKDFL-HGASHMLRADGEL 182

Query: 192 HVTHKEGEPYDKWELVEEAMKMGLKLQEAVPFRRGDYPGYENKRADGGQSDEPFRLGDCS 226
                                          F + +YPGYENKR DG + D+PF LG+CS
Sbjct: 183 EA-----------------------------FEKRNYPGYENKRGDGSRCDQPFLLGECS 217

[Close]

BLAST of MS010691 vs. TAIR 10
Match: AT4G26485.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 145.2 bits (365), Expect = 6.5e-35
Identity = 79/168 (47.02%), Postives = 104/168 (61.90%), Query Frame = 0


Query: 60  NIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYN 119
           NI ATSLDS+D+L  KY +A+ N+  L+  GC + + +D   MS    L  QR+DRIV+N
Sbjct: 2   NITATSLDSEDELSIKYMDAVDNINILKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFN 61

Query: 120 FPHVG--FLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVE 179
           FPH G  F  RE S   I+ +KELV GFL N K +L++ DGEIH+THK   P+  W + +
Sbjct: 62  FPHAGSRFFGRELSSRAIESHKELVRGFLENAKEMLEE-DGEIHITHKTTYPFSDWGIKK 121

Query: 180 EAMKMGLKLQEAVPFRRGDYPGYENKRADGG-QSDEPFRLGDCSTYKF 225
                GLKL +   F    YPGY  KR  GG +SD+ F +G+CSTY F
Sbjct: 122 LGKGEGLKLLKKSKFELSHYPGYITKRGSGGRRSDDYFPVGECSTYMF 168

The following BLAST results are available for this feature:

BLAST of MS010691 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132615.1	1.1e-124	98.71	uncharacterized protein At4g26485-like [Momordica charantia]	[more]
XP_023003736.1	3.6e-104	85.27	uncharacterized protein At4g26485-like [Cucurbita maxima]	[more]
XP_022926856.1	4.7e-104	84.82	uncharacterized protein At4g26485-like [Cucurbita moschata]	[more]
XP_011658099.1	8.0e-104	83.04	uncharacterized protein At4g26485 [Cucumis sativus] >KGN49019.1 hypothetical pro...	[more]
XP_038881536.1	8.0e-104	82.97	uncharacterized protein At4g26485-like [Benincasa hispida]	[more]

BLAST of MS010691 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I1X0	1.1e-52	50.00	Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX...	[more]
P0C8L4	1.5e-49	51.46	Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 P...	[more]
O94480	1.3e-19	33.33	25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 9...	[more]
Q3UY23	5.9e-17	33.00	Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus mus...	[more]
Q9BRP7	1.8e-13	31.64	Ferredoxin-fold anticodon-binding domain-containing protein 1 OS=Homo sapiens OX...	[more]

BLAST of MS010691 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BSY8	5.2e-125	98.71	uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1KQ33	1.7e-104	85.27	uncharacterized protein At4g26485-like OS=Cucurbita maxima OX=3661 GN=LOC1114972...	[more]
A0A6J1EG15	2.3e-104	84.82	uncharacterized protein At4g26485-like OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A0A0KJQ9	3.9e-104	83.04	DUF2431 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G510860 PE=...	[more]
A0A5D3CMV7	5.1e-104	81.36	DUF2431 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of MS010691 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55790.1	8.1e-54	50.00	Domain of unknown function (DUF2431)	[more]
AT5G56060.1	4.6e-41	45.50	Domain of unknown function (DUF2431)	[more]
AT5G56075.1	2.2e-38	42.65	Domain of unknown function (DUF2431)	[more]
AT1G55800.1	6.3e-38	38.78	Domain of unknown function (DUF2431)	[more]
AT4G26485.1	6.5e-35	47.02	Domain of unknown function (DUF2431)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 4..24
None	No IPR available	COILS	Coil	Coil	coord: 69..89
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 10..24
None	No IPR available	PANTHER	PTHR11538:SF63	NUCLEIC ACID BINDING, RELATED	coord: 6..226
None	No IPR available	PANTHER	PTHR11538	PHENYLALANYL-TRNA SYNTHETASE	coord: 6..226
IPR019446	Domain of unknown function DUF2431	PFAM	PF10354	DUF2431	coord: 38..204 e-value: 3.1E-52 score: 177.2
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 33..197

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010691.1	MS010691.1	mRNA