MS010577 (gene) Bitter gourd (TR) v1

Overview
NameMS010577
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionrRNA adenine N(6)-methyltransferase
Locationscaffold35: 678632 .. 679832 (-)
RNA-Seq ExpressionMS010577
SyntenyMS010577
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTAGTCCCCACAAAAATCTATGCAGGTTCGTCTATGGCCTGAACCACGACCATTCTTTTCACCGTACACGTTCAGCATCCTCTTACCAATACAACCAGAGAAACCATGGTGCGAACGAAAATGTTCGAACGAAAGACGGGAACGAAGAAGCCCAATTGTATTTCTACAAGAGTAGAGGCCAACACATTCTCACGAACCAGCGAATTCTGGACTCTATTGTTCGAAAATCTGCCATAAGGCCGAGCGACACAGTCTTGGAGATCGGACCCGGCACCGGCAATCTGACATTGAAGCTCCTAGAAGCTTCCCAAAAGGTCATAGCGATTGAAATCGACAAACGAATGGTCGAGATTCTTCACAGACGCGTGGTGGAACATAAATTTGAAGACCGCATTTGTGTAAGTTAAAATTGCAATCAATGTGTTCGATGAAATGCCTGACAGAGGATAAACTGCTGGAAGTGGGGTTTTGTAGGTCATATGCAAAGATGCTTTGAAGTGCGAGTTTCCTCGTTTCGACCTCGTCGTGGCCAACATCCCTTATGGCATATCTTCGCCGCTTGTGGCCAAGTTGGTATACGGGGCAATTCCATTTCGGAGCGCCACGCTTCTCCTTCAGAAAGAGTTTGCTCGGCGGCTTTTGGCGGATCCTGGTGACTCGGAGTACAACCGTTTGGCTGTGAATGTGAAGCTGGTGGCTGATGTGGAGTATGTCATGGATGTAAGCAAGAGGGAGTTTGTTCCTTGTCCGAAAGTCGACTCATCCGTGGTCATAATCCGCCCCAAGAAGGAAGTTCCAGAAGTGAACCTGGAAGAGTGGCGGTCTTTCACGAGAACTTGCTTTGGCAAGAAGAATAGAACACTTGGGGCAACCTTCAAGCAGAAGAGGAAGGTGATAGAGCTAATCCATTCATCAAAAGTGGCCTCAAATTCTGAGAATTCCATTGGTGGGAACAATGAAGAAAAGTCATCTTCTTCATCTTCGGAGAAGGGGTTTGATTCTTTCAAGGAGAGGATCATAGAAGTTCTAAAATCTGGAGATTTTGAAGATAAGAGGCCTGCAAAGTTGTGTAACAAGGAGTTGCTGCATTTGCTGGCGTTGTTTAATCGAGCTGGAATATATTTTCACGATGGAGCAGAGCTCCAGGATGCAGAAAGAAGAGCCTTAGCTGATGCCTACTGTTCAGAATCAGAACAG

mRNA sequence

ATGCTTAGTCCCCACAAAAATCTATGCAGGTTCGTCTATGGCCTGAACCACGACCATTCTTTTCACCGTACACGTTCAGCATCCTCTTACCAATACAACCAGAGAAACCATGGTGCGAACGAAAATGTTCGAACGAAAGACGGGAACGAAGAAGCCCAATTGTATTTCTACAAGAGTAGAGGCCAACACATTCTCACGAACCAGCGAATTCTGGACTCTATTGTTCGAAAATCTGCCATAAGGCCGAGCGACACAGTCTTGGAGATCGGACCCGGCACCGGCAATCTGACATTGAAGCTCCTAGAAGCTTCCCAAAAGGTCATAGCGATTGAAATCGACAAACGAATGGTCGAGATTCTTCACAGACGCGTGGTGGAACATAAATTTGAAGACCGCATTTGTGTCATATGCAAAGATGCTTTGAAGTGCGAGTTTCCTCGTTTCGACCTCGTCGTGGCCAACATCCCTTATGGCATATCTTCGCCGCTTGTGGCCAAGTTGGTATACGGGGCAATTCCATTTCGGAGCGCCACGCTTCTCCTTCAGAAAGAGTTTGCTCGGCGGCTTTTGGCGGATCCTGGTGACTCGGAGTACAACCGTTTGGCTGTGAATGTGAAGCTGGTGGCTGATGTGGAGTATGTCATGGATGTAAGCAAGAGGGAGTTTGTTCCTTGTCCGAAAGTCGACTCATCCGTGGTCATAATCCGCCCCAAGAAGGAAGTTCCAGAAGTGAACCTGGAAGAGTGGCGGTCTTTCACGAGAACTTGCTTTGGCAAGAAGAATAGAACACTTGGGGCAACCTTCAAGCAGAAGAGGAAGGTGATAGAGCTAATCCATTCATCAAAAGTGGCCTCAAATTCTGAGAATTCCATTGGTGGGAACAATGAAGAAAAGTCATCTTCTTCATCTTCGGAGAAGGGGTTTGATTCTTTCAAGGAGAGGATCATAGAAGTTCTAAAATCTGGAGATTTTGAAGATAAGAGGCCTGCAAAGTTGTGTAACAAGGAGTTGCTGCATTTGCTGGCGTTGTTTAATCGAGCTGGAATATATTTTCACGATGGAGCAGAGCTCCAGGATGCAGAAAGAAGAGCCTTAGCTGATGCCTACTGTTCAGAATCAGAACAG

Coding sequence (CDS)

ATGCTTAGTCCCCACAAAAATCTATGCAGGTTCGTCTATGGCCTGAACCACGACCATTCTTTTCACCGTACACGTTCAGCATCCTCTTACCAATACAACCAGAGAAACCATGGTGCGAACGAAAATGTTCGAACGAAAGACGGGAACGAAGAAGCCCAATTGTATTTCTACAAGAGTAGAGGCCAACACATTCTCACGAACCAGCGAATTCTGGACTCTATTGTTCGAAAATCTGCCATAAGGCCGAGCGACACAGTCTTGGAGATCGGACCCGGCACCGGCAATCTGACATTGAAGCTCCTAGAAGCTTCCCAAAAGGTCATAGCGATTGAAATCGACAAACGAATGGTCGAGATTCTTCACAGACGCGTGGTGGAACATAAATTTGAAGACCGCATTTGTGTCATATGCAAAGATGCTTTGAAGTGCGAGTTTCCTCGTTTCGACCTCGTCGTGGCCAACATCCCTTATGGCATATCTTCGCCGCTTGTGGCCAAGTTGGTATACGGGGCAATTCCATTTCGGAGCGCCACGCTTCTCCTTCAGAAAGAGTTTGCTCGGCGGCTTTTGGCGGATCCTGGTGACTCGGAGTACAACCGTTTGGCTGTGAATGTGAAGCTGGTGGCTGATGTGGAGTATGTCATGGATGTAAGCAAGAGGGAGTTTGTTCCTTGTCCGAAAGTCGACTCATCCGTGGTCATAATCCGCCCCAAGAAGGAAGTTCCAGAAGTGAACCTGGAAGAGTGGCGGTCTTTCACGAGAACTTGCTTTGGCAAGAAGAATAGAACACTTGGGGCAACCTTCAAGCAGAAGAGGAAGGTGATAGAGCTAATCCATTCATCAAAAGTGGCCTCAAATTCTGAGAATTCCATTGGTGGGAACAATGAAGAAAAGTCATCTTCTTCATCTTCGGAGAAGGGGTTTGATTCTTTCAAGGAGAGGATCATAGAAGTTCTAAAATCTGGAGATTTTGAAGATAAGAGGCCTGCAAAGTTGTGTAACAAGGAGTTGCTGCATTTGCTGGCGTTGTTTAATCGAGCTGGAATATATTTTCACGATGGAGCAGAGCTCCAGGATGCAGAAAGAAGAGCCTTAGCTGATGCCTACTGTTCAGAATCAGAACAG

Protein sequence

MLSPHKNLCRFVYGLNHDHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSRGQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEILHRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLLLQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKEVPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSSSSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDAERRALADAYCSESEQ
Homology
BLAST of MS010577 vs. NCBI nr
Match: XP_022132507.1 (ribosomal RNA small subunit methyltransferase, mitochondrial [Momordica charantia])

HSP 1 Score: 728.8 bits (1880), Expect = 2.4e-206
Identity = 373/375 (99.47%), Postives = 375/375 (100.00%), Query Frame = 0

Query: 1   MLSPHKNLCRFVYGLNHDHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60
           MLSPHKNLCRFVYGLNH+HSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR
Sbjct: 1   MLSPHKNLCRFVYGLNHNHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60

Query: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120
           GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL
Sbjct: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120

Query: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300
           VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS
Sbjct: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300

Query: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360
           SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA
Sbjct: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360

Query: 361 ERRALADAYCSESEQ 376
           ERR+LADAYCSESEQ
Sbjct: 361 ERRSLADAYCSESEQ 375

BLAST of MS010577 vs. NCBI nr
Match: KAG6594800.1 (Ribosomal RNA small subunit methyltransferase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 609.0 bits (1569), Expect = 2.8e-170
Identity = 319/375 (85.07%), Postives = 339/375 (90.40%), Query Frame = 0

Query: 1   MLSPHKNLCRFVYGLNHDHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60
           ML PHKNLCRF+ GLNHD   H  RSASSYQYN RNHG  ENV  KDGNEE QLYFYKSR
Sbjct: 1   MLRPHKNLCRFINGLNHDQFLHCKRSASSYQYNHRNHGGKENVPKKDGNEEPQLYFYKSR 60

Query: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120
           GQHILTN RIL SIVRKSAIRP+DTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVE+L
Sbjct: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120

Query: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRV EHKFEDRICVI +DALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HRRVAEHKFEDRICVIREDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLA+PGDSEYNRLAVNVKLVADV +VMDVSKR+FVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVAHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300
           VPEVNLEEWRSFTRTCFGKKN+TLGATFKQKR ++ L++     SNSE+  GG  EE+  
Sbjct: 241 VPEVNLEEWRSFTRTCFGKKNKTLGATFKQKRMLMALMN-----SNSESFNGGGVEEEGC 300

Query: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360
           SSSSEKGF+SFKE+IIE+LKSG FEDKRPAKLCN+ELLHLLALFN+AGIYF DG+ELQDA
Sbjct: 301 SSSSEKGFNSFKEKIIEILKSGSFEDKRPAKLCNEELLHLLALFNQAGIYFRDGSELQDA 360

Query: 361 ERRALA-DAYCSESE 375
           ERRALA DAY SE E
Sbjct: 361 ERRALADDAYGSELE 370

BLAST of MS010577 vs. NCBI nr
Match: XP_022963244.1 (ribosomal RNA small subunit methyltransferase, mitochondrial [Cucurbita moschata])

HSP 1 Score: 605.9 bits (1561), Expect = 2.3e-169
Identity = 318/375 (84.80%), Postives = 338/375 (90.13%), Query Frame = 0

Query: 1   MLSPHKNLCRFVYGLNHDHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60
           ML PHKNLCRF+ GLNHD   H  RSASSYQYN RNHG  ENV  KDGNEE QLYFYKSR
Sbjct: 1   MLRPHKNLCRFINGLNHDQFLHCKRSASSYQYNHRNHGGKENVPKKDGNEEPQLYFYKSR 60

Query: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120
           GQHILTN RIL SIVRKSAIRP+DTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVE+L
Sbjct: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120

Query: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRV EHKFEDRICVI +DALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HRRVAEHKFEDRICVIREDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLA+PGDSEYNRLAVNVKLVADVE+VMDVSKR+FVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300
           VPEVNLEEWRSFTRTCFGKKN+TLGATFKQKR ++ L++     SNSE+  G   EE+  
Sbjct: 241 VPEVNLEEWRSFTRTCFGKKNKTLGATFKQKRMLMALMN-----SNSESFNGQGVEEEGC 300

Query: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360
           SSSSEKGF+SFK +IIE+LKSG FEDKRPAKLCN+ELLHLLALFN+AGIYF DG+ELQDA
Sbjct: 301 SSSSEKGFNSFKAKIIEILKSGSFEDKRPAKLCNEELLHLLALFNQAGIYFRDGSELQDA 360

Query: 361 ERRALA-DAYCSESE 375
           ERRALA DAY SE E
Sbjct: 361 ERRALADDAYGSELE 370

BLAST of MS010577 vs. NCBI nr
Match: XP_023517344.1 (ribosomal RNA small subunit methyltransferase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 604.7 bits (1558), Expect = 5.2e-169
Identity = 316/375 (84.27%), Postives = 338/375 (90.13%), Query Frame = 0

Query: 1   MLSPHKNLCRFVYGLNHDHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60
           ML PHKNLCRF+ GLNHD   H  RSASSYQYN RNHG  ENV  KDGNEE QLYFYKSR
Sbjct: 1   MLRPHKNLCRFINGLNHDQFLHCKRSASSYQYNHRNHGEKENVPKKDGNEEPQLYFYKSR 60

Query: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120
           GQHILTN RIL SIVRKSAI+P+DTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVE+L
Sbjct: 61  GQHILTNPRILGSIVRKSAIKPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120

Query: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRV EHKFEDRICVI +DALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HRRVAEHKFEDRICVIREDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLA+PGDSEYNRLAVNVKLVADVE+VMDVSKR+FVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300
           +PEVNLEEWRSFTRTCFGKKN+TLGATFKQKR ++ L++     SNSE+  G   EE+  
Sbjct: 241 IPEVNLEEWRSFTRTCFGKKNKTLGATFKQKRMLMALMN-----SNSESFNGRGVEEEGC 300

Query: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360
           SSSSEKGF+SFKE+IIE+LKSG FEDKRPAKLCN+E LHLLALFN+AGIYF DG+ELQDA
Sbjct: 301 SSSSEKGFNSFKEKIIEILKSGSFEDKRPAKLCNEEFLHLLALFNQAGIYFRDGSELQDA 360

Query: 361 ERRALA-DAYCSESE 375
           ERRALA DAY SE E
Sbjct: 361 ERRALADDAYGSELE 370

BLAST of MS010577 vs. NCBI nr
Match: KAG7026768.1 (Ribosomal RNA small subunit methyltransferase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 604.7 bits (1558), Expect = 5.2e-169
Identity = 316/375 (84.27%), Postives = 338/375 (90.13%), Query Frame = 0

Query: 1   MLSPHKNLCRFVYGLNHDHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60
           ML PHKNLCRF+ GLNHD   H  RSASSYQYN RNHG  ENV  KDGNEE QLYFYKSR
Sbjct: 1   MLRPHKNLCRFINGLNHDQFLHCKRSASSYQYNHRNHGGKENVPKKDGNEEPQLYFYKSR 60

Query: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120
           GQHILTN RIL  IVRKSA+RP+DTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVE+L
Sbjct: 61  GQHILTNPRILGFIVRKSAVRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120

Query: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           H+RV EHKFEDRICVI +DALKCEFP FDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HKRVAEHKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLA+PGDSEYNRLAVNVKLVADVE+VMDVSKR+FVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300
           VPEVNLEEWRSFTRTCFGKKN+TLGATFKQKR ++ L++     SNSE+  GG  EE+  
Sbjct: 241 VPEVNLEEWRSFTRTCFGKKNKTLGATFKQKRMLMALMN-----SNSESFNGGGVEEEGC 300

Query: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360
           SSSSEKGF+SFKE+IIE+LKSG FEDKRPAKLCN+ELLHLLALFN+AGIYF DG+ELQDA
Sbjct: 301 SSSSEKGFNSFKEKIIEILKSGSFEDKRPAKLCNEELLHLLALFNQAGIYFRDGSELQDA 360

Query: 361 ERRALA-DAYCSESE 375
           ERRALA DAY SE E
Sbjct: 361 ERRALADDAYGSELE 370

BLAST of MS010577 vs. ExPASy Swiss-Prot
Match: Q9FK02 (Ribosomal RNA small subunit methyltransferase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DIM1B PE=1 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 2.0e-110
Identity = 217/354 (61.30%), Postives = 273/354 (77.12%), Query Frame = 0

Query: 19  HSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSRGQHILTNQRILDSIVRKS 78
           HS  RT+      +++R  G   +VR ++  E   L+  KS+GQH+LTN RILDSIVR S
Sbjct: 30  HSQARTKP----DHDRRRRGYERDVRIEEKKEHDGLFLCKSKGQHLLTNTRILDSIVRSS 89

Query: 79  AIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEILHRRVVEHKFEDRICVICK 138
            IRP+DTVLEIGPGTGNLT+KLLEA+Q V+A+E+DKRMVEIL +RV +H F D++ +I K
Sbjct: 90  DIRPTDTVLEIGPGTGNLTMKLLEAAQNVVAVELDKRMVEILRKRVSDHGFADKLTIIQK 149

Query: 139 DALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLLLQKEFARRLLADPGDSEY 198
           D LK +FP FDLVVANIPY ISSPLVAKLVYG+  FRSATLLLQKEF+RRLLA+PGDS++
Sbjct: 150 DVLKTDFPHFDLVVANIPYNISSPLVAKLVYGSNTFRSATLLLQKEFSRRLLANPGDSDF 209

Query: 199 NRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKEVPEVNLEEWRSFTRTCFG 258
           NRLAVNVKLVADV++VMDVSKREFVP PKVDSSV+ I PK+ +P+VN++EW +FTRTCFG
Sbjct: 210 NRLAVNVKLVADVKFVMDVSKREFVPPPKVDSSVIRITPKEIIPDVNVQEWLAFTRTCFG 269

Query: 259 KKNRTLGATFKQKRKVIEL--IHSSKVASNSE--NSIGGNNEEKSSSSSSEKGFDS---- 318
           KKN+TLG+ F+QK+KV+EL  + + +  SN E  N  GG+    S S   E G D     
Sbjct: 270 KKNKTLGSMFRQKKKVMELQSLSAGRHGSNVEVMNQTGGD----SDSDVEEDGKDDLLCL 329

Query: 319 ------FKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQ 359
                 FKER+IE+L++  FE+KRP+KL ++ELLHLL+LFN+AGI+FHD   LQ
Sbjct: 330 DTDASMFKERVIEILRTNGFEEKRPSKLSHRELLHLLSLFNQAGIFFHDITSLQ 375

BLAST of MS010577 vs. ExPASy Swiss-Prot
Match: O22268 (Ribosomal RNA small subunit methyltransferase OS=Arabidopsis thaliana OX=3702 GN=DIM1A PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.2e-70
Identity = 153/337 (45.40%), Postives = 209/337 (62.02%), Query Frame = 0

Query: 44  RTKDGNEEAQLYFYKSRGQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEA 103
           R    + +  + F+KS+GQHIL N  ++DSIV+K+ I+ +D +LEIGPGTGNLT KLLEA
Sbjct: 16  RAPSNHYQGGISFHKSKGQHILKNPLLVDSIVQKAGIKSTDVILEIGPGTGNLTKKLLEA 75

Query: 104 SQKVIAIEIDKRMVEILHRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPL 163
            ++VIA+E+D RMV  L RR     F +R+ VI  D LK E PRFD+ VANIPY ISSPL
Sbjct: 76  GKEVIAVELDSRMVLELQRRFQGTPFSNRLKVIQGDVLKTELPRFDICVANIPYQISSPL 135

Query: 164 VAKLVYGAIPFRSATLLLQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFV 223
             KL++    FR A ++ Q+EFA RL+A PGD+ Y RL+VN +L A V +++ V K  F 
Sbjct: 136 TFKLLFHPTSFRCAVIMYQREFAMRLVAQPGDNLYCRLSVNTQLYARVSHLLKVGKNNFR 195

Query: 224 PCPKVDSSVVIIRPKKEVPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKV 283
           P PKVDSSVV I P++  P+VN +EW  F R CF +KN+TLG+ FKQK  +  L  + K 
Sbjct: 196 PPPKVDSSVVRIEPRRPGPQVNKKEWDGFLRVCFIRKNKTLGSIFKQKSVLSMLEKNFKT 255

Query: 284 ASNSENSIGGNNEEKSSSSSSEKGFDS-----------------------------FKER 343
                 S+  N E   +++S + G  S                             FKE+
Sbjct: 256 LQAVLASLQNNGEPALNTTSMDLGDQSMGMEDDDNEMDDDDMEMDEGEGDGGETSEFKEK 315

Query: 344 IIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYF 352
           ++ VLK G FE+KR +KL  +E L+LL+LFN++GI+F
Sbjct: 316 VMNVLKEGGFEEKRSSKLSQQEFLYLLSLFNKSGIHF 352

BLAST of MS010577 vs. ExPASy Swiss-Prot
Match: Q54QK7 (Probable dimethyladenosine transferase OS=Dictyostelium discoideum OX=44689 GN=dimt1 PE=3 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 6.6e-58
Identity = 131/311 (42.12%), Postives = 178/311 (57.23%), Query Frame = 0

Query: 41  ENVRTKDGNEEAQLYFYKSRGQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKL 100
           E  ++       +    KS GQH+L N  I+D+IV KS ++ +DTVLEIGPGTGNLT+KL
Sbjct: 14  EKTKSATAARHHEFQMNKSYGQHLLKNPLIIDAIVDKSQLKSTDTVLEIGPGTGNLTMKL 73

Query: 101 LEASQKVIAIEIDKRMVEILHRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGIS 160
           LE  +KVIAIE+D RM   L +RV    +   + +I  D LK + P FD+ VAN+PY IS
Sbjct: 74  LENCKKVIAIEVDPRMAAELQKRVAASPYAQHLQIILGDFLKVDLPYFDVCVANVPYQIS 133

Query: 161 SPLVAKLVYGAIPFRSATLLLQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKR 220
           SPL  KL+     FR+A L+ QKEFA RL A PGDS Y RL+VN +L++ V ++M V K 
Sbjct: 134 SPLTFKLLAHRPIFRTAVLMFQKEFALRLGAKPGDSLYCRLSVNTQLLSKVTHLMKVGKN 193

Query: 221 EFVPCPKVDSSVVIIRPKKEVPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHS 280
            F+P PKV+S+VV I P    P +N  EW    + CF +KN+TL   F+    +  L  +
Sbjct: 194 NFLPPPKVESAVVRIEPFNPPPPINFVEWDGLVKLCFSRKNKTLSGIFRVSSVIETLNQN 253

Query: 281 SKVASNSENSIGGNNEEKSSSSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHL 340
            K     E  +  +  +           +  KE II+ L   DF D R +KL   + L L
Sbjct: 254 YKTYCALEGKMNTDGSD-----------EQMKELIIKTLTDNDFLDSRSSKLDINDFLKL 313

Query: 341 LALFNRAGIYF 352
           L  F+  GI+F
Sbjct: 314 LNKFHETGIHF 313

BLAST of MS010577 vs. ExPASy Swiss-Prot
Match: Q9VAQ5 (Probable dimethyladenosine transferase OS=Drosophila melanogaster OX=7227 GN=CG11837 PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.2e-56
Identity = 128/301 (42.52%), Postives = 181/301 (60.13%), Query Frame = 0

Query: 54  LYFYKSRGQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEID 113
           + F K  GQHIL N  ++ +++ K+A+R +D VLEIGPGTGN+T+++LE ++KVIA EID
Sbjct: 21  IVFNKDFGQHILKNPLVITTMLEKAALRATDVVLEIGPGTGNMTVRMLERAKKVIACEID 80

Query: 114 KRMVEILHRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIP 173
            R+   L +RV     + ++ V+  D LK E P FDL +AN+PY ISSPL+ KL+     
Sbjct: 81  TRLAAELQKRVQATPLQPKLQVLIGDFLKAELPFFDLCIANVPYQISSPLIFKLLLHRPL 140

Query: 174 FRSATLLLQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVV 233
           FR A L+ Q+EFA RL+A PGD  Y RL++N +L+A V+ +M V K  F P PKV+SSVV
Sbjct: 141 FRCAVLMFQREFAERLVAKPGDKLYCRLSINTQLLARVDMLMKVGKNNFRPPPKVESSVV 200

Query: 234 IIRPKKEVPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGG 293
            + PK   P VN  EW   TR  F +KN+TL ATFK    V+E++               
Sbjct: 201 RLEPKNPPPPVNFTEWDGLTRIAFLRKNKTLAATFK-VTSVLEMLE-------------- 260

Query: 294 NNEEKSSSSSSEKGFDSFK--ERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYF 353
            N +   S  +E   D FK  +++I +L+  D   KR   +   + + LL  FN AGI+F
Sbjct: 261 KNYKLYRSLRNEPIEDDFKMQDKVISILEEQDMAAKRARSMDIDDFMRLLLAFNSAGIHF 306

BLAST of MS010577 vs. ExPASy Swiss-Prot
Match: Q09522 (Probable dimethyladenosine transferase OS=Caenorhabditis elegans OX=6239 GN=E02H1.1 PE=3 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 2.1e-56
Identity = 136/313 (43.45%), Postives = 190/313 (60.70%), Query Frame = 0

Query: 44  RTKDGNEEA---QLYFYKSRGQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKL 103
           +TK G+       L F   +GQHIL N  ++++IV KSA++ +DTVLE+GPGTGNLT+K+
Sbjct: 9   KTKAGSSTGNVQSLPFNTDKGQHILKNPGVVNAIVEKSALKATDTVLEVGPGTGNLTVKM 68

Query: 104 LEASQKVIAIEIDKRMVEILHRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGIS 163
           LE ++ VIA EID RM+  + +RV+    ++++ V   D +K E+P FD+ VAN+PY IS
Sbjct: 69  LEVAKTVIACEIDPRMIAEVKKRVMGTPLQNKLQVNGGDVMKMEWPFFDVCVANLPYQIS 128

Query: 164 SPLVAKLVYGAIPFRSATLLLQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKR 223
           SP V KL+      R A L+ QKEFA RL+A PGD +Y+RL+VNV+L+A VE +M V + 
Sbjct: 129 SPFVQKLLLHRPLPRYAVLMFQKEFADRLVARPGDKDYSRLSVNVQLLAKVEMLMKVKRT 188

Query: 224 EFVPCPKVDSSVVIIRPKKEVPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHS 283
           EF P PKVDS+VV I PK   P VN  EW    R CF +KN+TL A F+    VIE+I  
Sbjct: 189 EFRPPPKVDSAVVRIAPKNPPPPVNFVEWEGLLRLCFMRKNKTLMAIFRLS-NVIEVIED 248

Query: 284 S--KVASNSENSIGGNNEEKSSSSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELL 343
           +  KV S     I  +               + K+ I E L +  + + R  K+  ++ L
Sbjct: 249 NFRKVCSFKNKPIPKDL--------------NMKKVIEETLTASGYGESRARKMRVEDFL 306

Query: 344 HLLALFNRAGIYF 352
            LL  FN+A I+F
Sbjct: 309 ALLLAFNKADIHF 306

BLAST of MS010577 vs. ExPASy TrEMBL
Match: A0A6J1BU06 (rRNA adenine N(6)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111005347 PE=3 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.2e-206
Identity = 373/375 (99.47%), Postives = 375/375 (100.00%), Query Frame = 0

Query: 1   MLSPHKNLCRFVYGLNHDHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60
           MLSPHKNLCRFVYGLNH+HSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR
Sbjct: 1   MLSPHKNLCRFVYGLNHNHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60

Query: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120
           GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL
Sbjct: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120

Query: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300
           VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS
Sbjct: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300

Query: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360
           SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA
Sbjct: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360

Query: 361 ERRALADAYCSESEQ 376
           ERR+LADAYCSESEQ
Sbjct: 361 ERRSLADAYCSESEQ 375

BLAST of MS010577 vs. ExPASy TrEMBL
Match: A0A6J1HJI5 (rRNA adenine N(6)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111463514 PE=3 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.1e-169
Identity = 318/375 (84.80%), Postives = 338/375 (90.13%), Query Frame = 0

Query: 1   MLSPHKNLCRFVYGLNHDHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60
           ML PHKNLCRF+ GLNHD   H  RSASSYQYN RNHG  ENV  KDGNEE QLYFYKSR
Sbjct: 1   MLRPHKNLCRFINGLNHDQFLHCKRSASSYQYNHRNHGGKENVPKKDGNEEPQLYFYKSR 60

Query: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120
           GQHILTN RIL SIVRKSAIRP+DTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVE+L
Sbjct: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120

Query: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRV EHKFEDRICVI +DALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HRRVAEHKFEDRICVIREDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLA+PGDSEYNRLAVNVKLVADVE+VMDVSKR+FVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300
           VPEVNLEEWRSFTRTCFGKKN+TLGATFKQKR ++ L++     SNSE+  G   EE+  
Sbjct: 241 VPEVNLEEWRSFTRTCFGKKNKTLGATFKQKRMLMALMN-----SNSESFNGQGVEEEGC 300

Query: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360
           SSSSEKGF+SFK +IIE+LKSG FEDKRPAKLCN+ELLHLLALFN+AGIYF DG+ELQDA
Sbjct: 301 SSSSEKGFNSFKAKIIEILKSGSFEDKRPAKLCNEELLHLLALFNQAGIYFRDGSELQDA 360

Query: 361 ERRALA-DAYCSESE 375
           ERRALA DAY SE E
Sbjct: 361 ERRALADDAYGSELE 370

BLAST of MS010577 vs. ExPASy TrEMBL
Match: A0A6J1KLE6 (rRNA adenine N(6)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111496791 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.9e-165
Identity = 314/375 (83.73%), Postives = 335/375 (89.33%), Query Frame = 0

Query: 1   MLSPHKNLCRFVYGLNHDHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60
           ML PHKNLCRF+  LNHD   H TRSASSYQYN RNHG  ENV  KDGNEE QLYFYKSR
Sbjct: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENV-PKDGNEEPQLYFYKSR 60

Query: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120
           GQHILTN RIL SIVRKSAIRP+DTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVE+L
Sbjct: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120

Query: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRV E+KFEDRICVI +DALKCEFP FDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLA+PGDSEYNRLAVNVKLVADVE+VMDVSKR+FVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300
           VPEVNLEEWR+FTRTCFGKKN+TLGATFKQKR ++ L++     SNSE+  G   EE+  
Sbjct: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMN-----SNSESFNGRGVEEEGC 300

Query: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360
           SSSSEKGF+ FKE+IIEVLKSG FEDKRPAKLCN +LLHLLALFN+AGIYF DG+ELQDA
Sbjct: 301 SSSSEKGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDA 360

Query: 361 ERRALA-DAYCSESE 375
           ER ALA DAY SE E
Sbjct: 361 ERSALADDAYGSELE 369

BLAST of MS010577 vs. ExPASy TrEMBL
Match: A0A0A0KJ94 (rRNA adenine N(6)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_6G504600 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 7.9e-163
Identity = 295/374 (78.88%), Postives = 326/374 (87.17%), Query Frame = 0

Query: 1   MLSPHKNLCRFVYGLNHDHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60
           ML PHKNLC F+YGLNH    H  RSAS+YQ+N  NHGAN N+R KD NEEA LYFYKSR
Sbjct: 1   MLRPHKNLCSFIYGLNHHQFLHLMRSASTYQHNHINHGANGNLRKKDENEEAHLYFYKSR 60

Query: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120
           GQHILTNQRILDSIVRKSAI P+DTVLEIGPGTGNLTLKLLEASQKVIA+EIDKRMVE+L
Sbjct: 61  GQHILTNQRILDSIVRKSAILPTDTVLEIGPGTGNLTLKLLEASQKVIAVEIDKRMVEVL 120

Query: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           H+RVVEH+ EDRICVIC+DALKCEFP FDLVVANIPYGISSPLVAKLVYG IPFRSATLL
Sbjct: 121 HKRVVEHQLEDRICVICQDALKCEFPHFDLVVANIPYGISSPLVAKLVYGTIPFRSATLL 180

Query: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLADPGDSEYNRLAVNVKLVADVE+VMDVSKR+FVPCPKVDSSVV+IRPKKE
Sbjct: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVLIRPKKE 240

Query: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300
           VPEVNLEEWR+FTRTCFGKKN+TLGATFKQKRK+++L+H                     
Sbjct: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRKLMDLMH--------------------- 300

Query: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360
            S+S +GF+SF+E +I+VLKSGDFEDKRP KLCN+ELLHLLALFN++GIYFH+ ++LQDA
Sbjct: 301 -SNSMEGFNSFRETVIQVLKSGDFEDKRPVKLCNEELLHLLALFNQSGIYFHNRSDLQDA 352

Query: 361 ERRALADAYCSESE 375
           ER  LADAY SE E
Sbjct: 361 ERSVLADAYSSEQE 352

BLAST of MS010577 vs. ExPASy TrEMBL
Match: A0A5A7SHR6 (rRNA adenine N(6)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253G002020 PE=3 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.9e-161
Identity = 293/374 (78.34%), Postives = 325/374 (86.90%), Query Frame = 0

Query: 1   MLSPHKNLCRFVYGLNHDHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60
           ML PHKNLCRF+YGLNH    H  RSAS+YQ+N RN+G + N+  KD NEEA LYFYKSR
Sbjct: 1   MLRPHKNLCRFIYGLNHHQFLHCIRSASTYQHNHRNNGPHGNLPKKDENEEAHLYFYKSR 60

Query: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120
           GQHILTNQRILDSIVRKSAI P+DTVLEIGPGTGNLTLKLLEASQKVIA+EIDKRMVE+L
Sbjct: 61  GQHILTNQRILDSIVRKSAILPTDTVLEIGPGTGNLTLKLLEASQKVIAVEIDKRMVEVL 120

Query: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRV EH+ EDRICVIC+DALKCE P FDLVVANIPYGISSPLVAKLVYG IPFRSATLL
Sbjct: 121 HRRVAEHQLEDRICVICQDALKCELPHFDLVVANIPYGISSPLVAKLVYGTIPFRSATLL 180

Query: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLADPGDSEYNRLAVNVKLVADVE+VMDVSKR+FVPCPKVDSSVV+IRPKKE
Sbjct: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVLIRPKKE 240

Query: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300
           VPEVNLEEWR+FTRTCFGKKN+TLGATFKQKRK+++L+H                     
Sbjct: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRKLMDLMH--------------------- 300

Query: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360
            S+SE+GF+SF+E +I+VLKSGDFEDKRP KLCN++LLHLLALFN++GIYFH+ +ELQDA
Sbjct: 301 -SNSEEGFNSFRETVIQVLKSGDFEDKRPVKLCNEKLLHLLALFNQSGIYFHNRSELQDA 352

Query: 361 ERRALADAYCSESE 375
           ER  LADAY SE E
Sbjct: 361 ERSVLADAYSSEQE 352

BLAST of MS010577 vs. TAIR 10
Match: AT5G66360.2 (Ribosomal RNA adenine dimethylase family protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.4e-111
Identity = 217/354 (61.30%), Postives = 273/354 (77.12%), Query Frame = 0

Query: 19  HSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSRGQHILTNQRILDSIVRKS 78
           HS  RT+      +++R  G   +VR ++  E   L+  KS+GQH+LTN RILDSIVR S
Sbjct: 30  HSQARTKP----DHDRRRRGYERDVRIEEKKEHDGLFLCKSKGQHLLTNTRILDSIVRSS 89

Query: 79  AIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEILHRRVVEHKFEDRICVICK 138
            IRP+DTVLEIGPGTGNLT+KLLEA+Q V+A+E+DKRMVEIL +RV +H F D++ +I K
Sbjct: 90  DIRPTDTVLEIGPGTGNLTMKLLEAAQNVVAVELDKRMVEILRKRVSDHGFADKLTIIQK 149

Query: 139 DALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLLLQKEFARRLLADPGDSEY 198
           D LK +FP FDLVVANIPY ISSPLVAKLVYG+  FRSATLLLQKEF+RRLLA+PGDS++
Sbjct: 150 DVLKTDFPHFDLVVANIPYNISSPLVAKLVYGSNTFRSATLLLQKEFSRRLLANPGDSDF 209

Query: 199 NRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKEVPEVNLEEWRSFTRTCFG 258
           NRLAVNVKLVADV++VMDVSKREFVP PKVDSSV+ I PK+ +P+VN++EW +FTRTCFG
Sbjct: 210 NRLAVNVKLVADVKFVMDVSKREFVPPPKVDSSVIRITPKEIIPDVNVQEWLAFTRTCFG 269

Query: 259 KKNRTLGATFKQKRKVIEL--IHSSKVASNSE--NSIGGNNEEKSSSSSSEKGFDS---- 318
           KKN+TLG+ F+QK+KV+EL  + + +  SN E  N  GG+    S S   E G D     
Sbjct: 270 KKNKTLGSMFRQKKKVMELQSLSAGRHGSNVEVMNQTGGD----SDSDVEEDGKDDLLCL 329

Query: 319 ------FKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQ 359
                 FKER+IE+L++  FE+KRP+KL ++ELLHLL+LFN+AGI+FHD   LQ
Sbjct: 330 DTDASMFKERVIEILRTNGFEEKRPSKLSHRELLHLLSLFNQAGIFFHDITSLQ 375

BLAST of MS010577 vs. TAIR 10
Match: AT5G66360.1 (Ribosomal RNA adenine dimethylase family protein )

HSP 1 Score: 343.2 bits (879), Expect = 2.6e-94
Identity = 194/354 (54.80%), Postives = 247/354 (69.77%), Query Frame = 0

Query: 19  HSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSRGQHILTNQRILDSIVRKS 78
           HS  RT+      +++R  G   +VR ++  E   L+  KS+GQH+LTN RILDSIVR S
Sbjct: 30  HSQARTKP----DHDRRRRGYERDVRIEEKKEHDGLFLCKSKGQHLLTNTRILDSIVRSS 89

Query: 79  AIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEILHRRVVEHKFEDRICVICK 138
            IRP+DTVLEIGPGTGNLT+KLLEA+Q V+A+E+DKRMVEIL +RV +H F D++ +I K
Sbjct: 90  DIRPTDTVLEIGPGTGNLTMKLLEAAQNVVAVELDKRMVEILRKRVSDHGFADKLTIIQK 149

Query: 139 DALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLLLQKEFARRLLADPGDSEY 198
           D LK +FP FDLVVANIPY ISSPLVAKLVYG+  FRSATLLLQKEF+RRLLA+PGDS++
Sbjct: 150 DVLKTDFPHFDLVVANIPYNISSPLVAKLVYGSNTFRSATLLLQKEFSRRLLANPGDSDF 209

Query: 199 NRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKEVPEVNLEEWRSFTRTCFG 258
           NRLAVNVK                            I PK+ +P+VN++EW +FTRTCFG
Sbjct: 210 NRLAVNVK----------------------------ITPKEIIPDVNVQEWLAFTRTCFG 269

Query: 259 KKNRTLGATFKQKRKVIEL--IHSSKVASNSE--NSIGGNNEEKSSSSSSEKGFDS---- 318
           KKN+TLG+ F+QK+KV+EL  + + +  SN E  N  GG+    S S   E G D     
Sbjct: 270 KKNKTLGSMFRQKKKVMELQSLSAGRHGSNVEVMNQTGGD----SDSDVEEDGKDDLLCL 329

Query: 319 ------FKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQ 359
                 FKER+IE+L++  FE+KRP+KL ++ELLHLL+LFN+AGI+FHD   LQ
Sbjct: 330 DTDASMFKERVIEILRTNGFEEKRPSKLSHRELLHLLSLFNQAGIFFHDITSLQ 347

BLAST of MS010577 vs. TAIR 10
Match: AT2G47420.1 (Ribosomal RNA adenine dimethylase family protein )

HSP 1 Score: 268.5 bits (685), Expect = 8.3e-72
Identity = 153/337 (45.40%), Postives = 209/337 (62.02%), Query Frame = 0

Query: 44  RTKDGNEEAQLYFYKSRGQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEA 103
           R    + +  + F+KS+GQHIL N  ++DSIV+K+ I+ +D +LEIGPGTGNLT KLLEA
Sbjct: 16  RAPSNHYQGGISFHKSKGQHILKNPLLVDSIVQKAGIKSTDVILEIGPGTGNLTKKLLEA 75

Query: 104 SQKVIAIEIDKRMVEILHRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPL 163
            ++VIA+E+D RMV  L RR     F +R+ VI  D LK E PRFD+ VANIPY ISSPL
Sbjct: 76  GKEVIAVELDSRMVLELQRRFQGTPFSNRLKVIQGDVLKTELPRFDICVANIPYQISSPL 135

Query: 164 VAKLVYGAIPFRSATLLLQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFV 223
             KL++    FR A ++ Q+EFA RL+A PGD+ Y RL+VN +L A V +++ V K  F 
Sbjct: 136 TFKLLFHPTSFRCAVIMYQREFAMRLVAQPGDNLYCRLSVNTQLYARVSHLLKVGKNNFR 195

Query: 224 PCPKVDSSVVIIRPKKEVPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKV 283
           P PKVDSSVV I P++  P+VN +EW  F R CF +KN+TLG+ FKQK  +  L  + K 
Sbjct: 196 PPPKVDSSVVRIEPRRPGPQVNKKEWDGFLRVCFIRKNKTLGSIFKQKSVLSMLEKNFKT 255

Query: 284 ASNSENSIGGNNEEKSSSSSSEKGFDS-----------------------------FKER 343
                 S+  N E   +++S + G  S                             FKE+
Sbjct: 256 LQAVLASLQNNGEPALNTTSMDLGDQSMGMEDDDNEMDDDDMEMDEGEGDGGETSEFKEK 315

Query: 344 IIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYF 352
           ++ VLK G FE+KR +KL  +E L+LL+LFN++GI+F
Sbjct: 316 VMNVLKEGGFEEKRSSKLSQQEFLYLLSLFNKSGIHF 352

BLAST of MS010577 vs. TAIR 10
Match: AT1G01860.1 (Ribosomal RNA adenine dimethylase family protein )

HSP 1 Score: 102.8 bits (255), Expect = 6.0e-22
Identity = 80/245 (32.65%), Postives = 118/245 (48.16%), Query Frame = 0

Query: 58  KSRGQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMV 117
           KS GQH + N  I D +   + ++  D VLEIGPGTG+LT  L+     V+AIE D  MV
Sbjct: 73  KSLGQHYMLNSDINDQLASAADVKEGDFVLEIGPGTGSLTNVLINLGATVLAIEKDPHMV 132

Query: 118 EILHRRVVEHKFEDRICVICKDALKCEFPRFDL-----------------VVANIPYGIS 177
           +++  R       D+  V+ +D +KC      L                 VV+N+P+ IS
Sbjct: 133 DLVSERFAG---SDKFKVLQEDFVKCHIRSHMLSILETRRLSHPDSALAKVVSNLPFNIS 192

Query: 178 SPLVAKLVYGAIPFRSATLLLQKEFARRLLADPG--DSEYNRLAVNVKLVADVEYVMDVS 237
           + +V  L+     F    LLLQ E A RL+ +P    SEY  + + +   ++ EY   V 
Sbjct: 193 TDVVKLLLPMGDIFSKVVLLLQDEAALRLV-EPALRTSEYRPINILINFYSEPEYNFRVP 252

Query: 238 KREFVPCPKVDSSVVIIRPK--KEVPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIE 282
           +  F P PKVD++VV  + K  ++ P+V      S T+  F   N       K  RK ++
Sbjct: 253 RENFFPQPKVDAAVVTFKLKHPRDYPDV------SSTKNFFSLVNSAFNGKRKMLRKSLQ 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132507.12.4e-20699.47ribosomal RNA small subunit methyltransferase, mitochondrial [Momordica charanti... [more]
KAG6594800.12.8e-17085.07Ribosomal RNA small subunit methyltransferase, mitochondrial, partial [Cucurbita... [more]
XP_022963244.12.3e-16984.80ribosomal RNA small subunit methyltransferase, mitochondrial [Cucurbita moschata... [more]
XP_023517344.15.2e-16984.27ribosomal RNA small subunit methyltransferase, mitochondrial [Cucurbita pepo sub... [more]
KAG7026768.15.2e-16984.27Ribosomal RNA small subunit methyltransferase, mitochondrial, partial [Cucurbita... [more]
Match NameE-valueIdentityDescription
Q9FK022.0e-11061.30Ribosomal RNA small subunit methyltransferase, mitochondrial OS=Arabidopsis thal... [more]
O222681.2e-7045.40Ribosomal RNA small subunit methyltransferase OS=Arabidopsis thaliana OX=3702 GN... [more]
Q54QK76.6e-5842.12Probable dimethyladenosine transferase OS=Dictyostelium discoideum OX=44689 GN=d... [more]
Q9VAQ51.2e-5642.52Probable dimethyladenosine transferase OS=Drosophila melanogaster OX=7227 GN=CG1... [more]
Q095222.1e-5643.45Probable dimethyladenosine transferase OS=Caenorhabditis elegans OX=6239 GN=E02H... [more]
Match NameE-valueIdentityDescription
A0A6J1BU061.2e-20699.47rRNA adenine N(6)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC1110053... [more]
A0A6J1HJI51.1e-16984.80rRNA adenine N(6)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11146351... [more]
A0A6J1KLE64.9e-16583.73rRNA adenine N(6)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111496791 ... [more]
A0A0A0KJ947.9e-16378.88rRNA adenine N(6)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_6G504600 P... [more]
A0A5A7SHR61.9e-16178.34rRNA adenine N(6)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
Match NameE-valueIdentityDescription
AT5G66360.21.4e-11161.30Ribosomal RNA adenine dimethylase family protein [more]
AT5G66360.12.6e-9454.80Ribosomal RNA adenine dimethylase family protein [more]
AT2G47420.18.3e-7245.40Ribosomal RNA adenine dimethylase family protein [more]
AT1G01860.16.0e-2232.65Ribosomal RNA adenine dimethylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020598Ribosomal RNA adenine methylase transferase, N-terminalSMARTSM00650rADcneu6coord: 70..239
e-value: 1.9E-77
score: 273.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 56..241
e-value: 2.3E-63
score: 215.2
NoneNo IPR availableGENE3D1.10.8.480coord: 242..352
e-value: 1.3E-28
score: 101.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 285..306
NoneNo IPR availablePANTHERPTHR11727:SF12RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE, MITOCHONDRIALcoord: 9..352
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 85..168
e-value: 1.02757E-10
score: 56.2842
IPR001737Ribosomal RNA adenine methyltransferase KsgA/ErmPFAMPF00398RrnaADcoord: 58..278
e-value: 1.2E-59
score: 201.6
IPR001737Ribosomal RNA adenine methyltransferase KsgA/ErmPANTHERPTHR11727DIMETHYLADENOSINE TRANSFERASEcoord: 9..352
IPR001737Ribosomal RNA adenine methyltransferase KsgA/ErmPROSITEPS51689SAM_RNA_A_N6_MTcoord: 56..348
score: 63.057426
IPR011530Ribosomal RNA adenine dimethylaseTIGRFAMTIGR00755TIGR00755coord: 57..342
e-value: 7.0E-71
score: 236.5
IPR020596Ribosomal RNA adenine methylase transferase, conserved sitePROSITEPS01131RRNA_A_DIMETHcoord: 86..113
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 55..272

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010577.1MS010577.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000154 rRNA modification
biological_process GO:0006364 rRNA processing
molecular_function GO:0000179 rRNA (adenine-N6,N6-)-dimethyltransferase activity
molecular_function GO:0008649 rRNA methyltransferase activity