MS010566 (gene) Bitter gourd (TR) v1

Overview
NameMS010566
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAAA-ATPase At2g18193-like
Locationscaffold35: 607387 .. 608936 (+)
RNA-Seq ExpressionMS010566
SyntenyMS010566
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGCTTTCAAAGAAATGCCGCAGTCCGCTTCTTCCCTGTTCGCGGCCTACGCCTCCTTCGCCACCTCTATGATGATGATCCGTTCTATAACCAACGACCTTCTTCCTCCCCAATTACTTTCCTTCATCTCCTCCATTTTCTTCTACATTTTCCCCCCGAAATCCACTCCTCAGACCACCCTCGTCATCGAGAAGAAGTCCAATTTCTCCAAGAACCAGGTCTATGAAGCTGCCGAGATCTACCTCCGTACAAAAATCAGCTCCTCAATGGACCGTCTCAAGGTCTCCAAAACCGGAAGGCAGAAGAAAGTTTCCCTCTCCATGGAAAAGGGCCAAGAAATTACTGATGATTTTGAGAACATTCACCTCACATGGCGATTCGTCTCCGTAGAAAAGAAGGAAGACAGTGAATACGCCAAAGAGAAATACCACTACGAACTTGTATTCGATAAGAAATTCACGGATACGATTATAGATTTCTATTTCCCTTACATCTTACGCAGAGCCAAGGAGATTAAAGAGCTGGATAACGTTGCCAAGCTCTGTAGCCAAGATTGTACCTACGATGACGATTCTGGTGGTAGCGATGGATGTCGAGGGAATTGGGGATCCATCGCTCTTGAACATCCGGCGACGTTTGATACTCTGGCGGTGGATCCTGATTTGAAGAAGATGATAATAGACGATTTGGATAGATTCGTCAAGAGGAAGGAATTCTATCGGAAGGTAGGGAAGGCTTGGAAGAGAGGCTATTTGTTGTACGGTCCGCCTGGTACGGGGAAATCCAGCTTAATCGCCGCCATGGCTAATTACCTCAAGTTTGACATCTACGATCTTGATCTCACGGATATCGACAGCAATTACAATCTACGAAGAGCATTACTCTCTACTACGAATCGCTCAATTTTGGTGATTGAGGATATAGATTGCAGCGTGAATTTGCAGAATCGGGAAAGCGACGAGAACAACGATGACTCTACCAGAAGCAGGGTAGGTTTAATTAATTTTCTCGATTTTTCCCCTCCGAATTGTTGGATTATACTCTTGGTAATCGAAATTGAAACGGAAATTTCTGTGATGATTTGCAGTTGACGCTATCCGGTATGCTGAATTTCATAGATGGATTGTGGTCGAGTTGCGGCGACGAGAGAATCATCGTCCTCACGACGAATCACAAGGAACGGTTGGACCCGGCGCTGTTACGACCAGGTCGAATGGACGTCCACATAAACTTGTCCTACTGCACCTCCAAGGCGTTCAAGGTCTTGGCCACCAATTACCTCAGCGGCGAAGCGACTGCTCATCCGCTGTACGAAGAAATCGAAGGCCTAATCGATGGCGCCAACGTGACTCCGGCGGAGGTTGCGGAAGAACTAATGAAGAGTAACGACATTGACACCGTCATGGAAGGATTGGCCAAGTTTGTGAGACTTAAGAGAGAGGAACAGAGTGGGGGAAATGAGGCGCCGGAGGAGGAAGAAGGCGATGAGATTGTGGAAGAAGAGCAAGAAGAAAAACTGAACAGAAAAAAGAACGGCGCCGGAGTT

mRNA sequence

ATGTTTGCTTTCAAAGAAATGCCGCAGTCCGCTTCTTCCCTGTTCGCGGCCTACGCCTCCTTCGCCACCTCTATGATGATGATCCGTTCTATAACCAACGACCTTCTTCCTCCCCAATTACTTTCCTTCATCTCCTCCATTTTCTTCTACATTTTCCCCCCGAAATCCACTCCTCAGACCACCCTCGTCATCGAGAAGAAGTCCAATTTCTCCAAGAACCAGGTCTATGAAGCTGCCGAGATCTACCTCCGTACAAAAATCAGCTCCTCAATGGACCGTCTCAAGGTCTCCAAAACCGGAAGGCAGAAGAAAGTTTCCCTCTCCATGGAAAAGGGCCAAGAAATTACTGATGATTTTGAGAACATTCACCTCACATGGCGATTCGTCTCCGTAGAAAAGAAGGAAGACAGTGAATACGCCAAAGAGAAATACCACTACGAACTTGTATTCGATAAGAAATTCACGGATACGATTATAGATTTCTATTTCCCTTACATCTTACGCAGAGCCAAGGAGATTAAAGAGCTGGATAACGTTGCCAAGCTCTGTAGCCAAGATTGTACCTACGATGACGATTCTGGTGGTAGCGATGGATGTCGAGGGAATTGGGGATCCATCGCTCTTGAACATCCGGCGACGTTTGATACTCTGGCGGTGGATCCTGATTTGAAGAAGATGATAATAGACGATTTGGATAGATTCGTCAAGAGGAAGGAATTCTATCGGAAGGTAGGGAAGGCTTGGAAGAGAGGCTATTTGTTGTACGGTCCGCCTGGTACGGGGAAATCCAGCTTAATCGCCGCCATGGCTAATTACCTCAAGTTTGACATCTACGATCTTGATCTCACGGATATCGACAGCAATTACAATCTACGAAGAGCATTACTCTCTACTACGAATCGCTCAATTTTGGTGATTGAGGATATAGATTGCAGCGTGAATTTGCAGAATCGGGAAAGCGACGAGAACAACGATGACTCTACCAGAAGCAGGTTGACGCTATCCGGTATGCTGAATTTCATAGATGGATTGTGGTCGAGTTGCGGCGACGAGAGAATCATCGTCCTCACGACGAATCACAAGGAACGGTTGGACCCGGCGCTGTTACGACCAGGTCGAATGGACGTCCACATAAACTTGTCCTACTGCACCTCCAAGGCGTTCAAGGTCTTGGCCACCAATTACCTCAGCGGCGAAGCGACTGCTCATCCGCTGTACGAAGAAATCGAAGGCCTAATCGATGGCGCCAACGTGACTCCGGCGGAGGTTGCGGAAGAACTAATGAAGAGTAACGACATTGACACCGTCATGGAAGGATTGGCCAAGTTTGTGAGACTTAAGAGAGAGGAACAGAGTGGGGGAAATGAGGCGCCGGAGGAGGAAGAAGGCGATGAGATTGTGGAAGAAGAGCAAGAAGAAAAACTGAACAGAAAAAAGAACGGCGCCGGAGTT

Coding sequence (CDS)

ATGTTTGCTTTCAAAGAAATGCCGCAGTCCGCTTCTTCCCTGTTCGCGGCCTACGCCTCCTTCGCCACCTCTATGATGATGATCCGTTCTATAACCAACGACCTTCTTCCTCCCCAATTACTTTCCTTCATCTCCTCCATTTTCTTCTACATTTTCCCCCCGAAATCCACTCCTCAGACCACCCTCGTCATCGAGAAGAAGTCCAATTTCTCCAAGAACCAGGTCTATGAAGCTGCCGAGATCTACCTCCGTACAAAAATCAGCTCCTCAATGGACCGTCTCAAGGTCTCCAAAACCGGAAGGCAGAAGAAAGTTTCCCTCTCCATGGAAAAGGGCCAAGAAATTACTGATGATTTTGAGAACATTCACCTCACATGGCGATTCGTCTCCGTAGAAAAGAAGGAAGACAGTGAATACGCCAAAGAGAAATACCACTACGAACTTGTATTCGATAAGAAATTCACGGATACGATTATAGATTTCTATTTCCCTTACATCTTACGCAGAGCCAAGGAGATTAAAGAGCTGGATAACGTTGCCAAGCTCTGTAGCCAAGATTGTACCTACGATGACGATTCTGGTGGTAGCGATGGATGTCGAGGGAATTGGGGATCCATCGCTCTTGAACATCCGGCGACGTTTGATACTCTGGCGGTGGATCCTGATTTGAAGAAGATGATAATAGACGATTTGGATAGATTCGTCAAGAGGAAGGAATTCTATCGGAAGGTAGGGAAGGCTTGGAAGAGAGGCTATTTGTTGTACGGTCCGCCTGGTACGGGGAAATCCAGCTTAATCGCCGCCATGGCTAATTACCTCAAGTTTGACATCTACGATCTTGATCTCACGGATATCGACAGCAATTACAATCTACGAAGAGCATTACTCTCTACTACGAATCGCTCAATTTTGGTGATTGAGGATATAGATTGCAGCGTGAATTTGCAGAATCGGGAAAGCGACGAGAACAACGATGACTCTACCAGAAGCAGGTTGACGCTATCCGGTATGCTGAATTTCATAGATGGATTGTGGTCGAGTTGCGGCGACGAGAGAATCATCGTCCTCACGACGAATCACAAGGAACGGTTGGACCCGGCGCTGTTACGACCAGGTCGAATGGACGTCCACATAAACTTGTCCTACTGCACCTCCAAGGCGTTCAAGGTCTTGGCCACCAATTACCTCAGCGGCGAAGCGACTGCTCATCCGCTGTACGAAGAAATCGAAGGCCTAATCGATGGCGCCAACGTGACTCCGGCGGAGGTTGCGGAAGAACTAATGAAGAGTAACGACATTGACACCGTCATGGAAGGATTGGCCAAGTTTGTGAGACTTAAGAGAGAGGAACAGAGTGGGGGAAATGAGGCGCCGGAGGAGGAAGAAGGCGATGAGATTGTGGAAGAAGAGCAAGAAGAAAAACTGAACAGAAAAAAGAACGGCGCCGGAGTT

Protein sequence

MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQTTLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFENIHLTWRFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVAKLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEFYRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTNRSILVIEDIDCSVNLQNRESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGANVTPAEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGDEIVEEEQEEKLNRKKNGAGV
Homology
BLAST of MS010566 vs. NCBI nr
Match: XP_022132940.1 (AAA-ATPase At2g18193-like [Momordica charantia])

HSP 1 Score: 933.7 bits (2412), Expect = 6.3e-268
Identity = 478/484 (98.76%), Postives = 479/484 (98.97%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQT 60
           MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSI FY FPPKS+PQ 
Sbjct: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSILFYFFPPKSSPQA 60

Query: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120
           TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE
Sbjct: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120

Query: 121 NIHLTWRFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180
           NIHLTWRFVSVEKKED EYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA
Sbjct: 121 NIHLTWRFVSVEKKEDGEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180

Query: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240
           KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF
Sbjct: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300
           YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN
Sbjct: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300

Query: 301 RSILVIEDIDCSVNLQNRESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNH 360
           RSILVIEDIDCSVNLQNRESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNH
Sbjct: 301 RSILVIEDIDCSVNLQNRESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNH 360

Query: 361 KERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGANVTP 420
           KERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYL GEATAHPLYEEIEGLIDGANVTP
Sbjct: 361 KERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLGGEATAHPLYEEIEGLIDGANVTP 420

Query: 421 AEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGDEIVEEEQEEKLNRKKN 480
           AEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGDEIVEEEQEEKLNRKKN
Sbjct: 421 AEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGDEIVEEEQEEKLNRKKN 480

Query: 481 GAGV 485
           GAGV
Sbjct: 481 GAGV 484

BLAST of MS010566 vs. NCBI nr
Match: XP_022962680.1 (AAA-ATPase At2g18193-like [Cucurbita moschata])

HSP 1 Score: 737.3 bits (1902), Expect = 8.7e-209
Identity = 383/474 (80.80%), Postives = 422/474 (89.03%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQT 60
           M  FKEMP SASSLFAAYASFATSMMMIRSITNDLLPPQL+SFISSIF Y FPPKS+PQT
Sbjct: 1   MLNFKEMPPSASSLFAAYASFATSMMMIRSITNDLLPPQLISFISSIFTYFFPPKSSPQT 60

Query: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120
           TLVIEKK+N++KNQV+EAAEIYLRTKIS SMDRLKVSKT RQ++VSLSMEK QEI D FE
Sbjct: 61  TLVIEKKTNYNKNQVFEAAEIYLRTKISPSMDRLKVSKTPRQQRVSLSMEKDQEIVDQFE 120

Query: 121 NIHLTWRFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180
           +IHL WRFV+ +KKE +E+ KEK HYELVFDKKF D ++DFY PYILRRAKEI E++NV+
Sbjct: 121 DIHLKWRFVAEKKKEGNEFVKEKRHYELVFDKKFMDKVVDFYLPYILRRAKEITEMENVS 180

Query: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240
           KLCSQ+ +Y DD  G + CRGNWGSI LEHPATFDTLA+DPDLKKMIIDDLDRF+KRKEF
Sbjct: 181 KLCSQNLSYSDDF-GDERCRGNWGSIGLEHPATFDTLAMDPDLKKMIIDDLDRFLKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300
           YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDI SN  LRR+LLST+N
Sbjct: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDICSNSELRRSLLSTSN 300

Query: 301 RSILVIEDIDCSVNLQNR----ESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVL 360
           RSILVIEDIDCSVNLQNR    +  ENNDD  RS+LTLSGMLNF+DGLWSSCGDERIIVL
Sbjct: 301 RSILVIEDIDCSVNLQNRANSDDESENNDD-YRSKLTLSGMLNFMDGLWSSCGDERIIVL 360

Query: 361 TTNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGA 420
           TTNHK+RLDPALLRPGRMDVHI+LSYC+SKAF+ L TNYL G A  HPLYEEIE L++ A
Sbjct: 361 TTNHKDRLDPALLRPGRMDVHIHLSYCSSKAFEALTTNYLGGGAIHHPLYEEIEALVEHA 420

Query: 421 NVTPAEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGDEIVEEE 471
           NVTPAEVAEELMK +DID VMEGLAKFV+ KREEQ+ GN A  EEEG+EIVEE+
Sbjct: 421 NVTPAEVAEELMKDDDIDIVMEGLAKFVKRKREEQNDGNNA-AEEEGNEIVEEQ 471

BLAST of MS010566 vs. NCBI nr
Match: XP_023517746.1 (AAA-ATPase At2g18193-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 736.5 bits (1900), Expect = 1.5e-208
Identity = 382/474 (80.59%), Postives = 425/474 (89.66%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQT 60
           MF+FK+MP SASSLFAAYASFATSMMMIRSITNDLLPPQL+SFISSIF Y FPPKS+PQT
Sbjct: 1   MFSFKDMPPSASSLFAAYASFATSMMMIRSITNDLLPPQLISFISSIFTYFFPPKSSPQT 60

Query: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120
           TLVIEKK+N++KNQV+EAAEIYLRTKIS SMDRLKVSKT RQ++VSLSMEK QEI D FE
Sbjct: 61  TLVIEKKTNYNKNQVFEAAEIYLRTKISPSMDRLKVSKTPRQQRVSLSMEKDQEIVDQFE 120

Query: 121 NIHLTWRFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180
           +IHL WRFV+ +KKE +E  KEK HYELVFDKKF D ++DFY PYIL+RAKEI E++NV+
Sbjct: 121 DIHLKWRFVAEKKKEGNESVKEKRHYELVFDKKFMDKVVDFYLPYILQRAKEITEMENVS 180

Query: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240
           KLCSQ+ +Y DD  G + CRGNWGSI LEHPATFDTLA+DPDLKKMIIDDLDRF+KRKEF
Sbjct: 181 KLCSQNLSYSDDF-GDERCRGNWGSIGLEHPATFDTLAMDPDLKKMIIDDLDRFLKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300
           YRKVGKAWKRGYLLYGPPGTGKSSL+AAMANYLKFDIYDLDLTDI SN +LRR+LLST+N
Sbjct: 241 YRKVGKAWKRGYLLYGPPGTGKSSLVAAMANYLKFDIYDLDLTDICSNSDLRRSLLSTSN 300

Query: 301 RSILVIEDIDCSVNLQNR----ESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVL 360
           RSILVIEDIDCSVNLQNR    +  ENNDD  RS+LTLSGMLNF+DGLWSSCGDERIIVL
Sbjct: 301 RSILVIEDIDCSVNLQNRANSDDESENNDD-YRSKLTLSGMLNFMDGLWSSCGDERIIVL 360

Query: 361 TTNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGA 420
           TTNHK+RLDPALLRPGRMDVHI+LSYCTSKAF+ LATNYL G A  HPLYEEIE L++ A
Sbjct: 361 TTNHKDRLDPALLRPGRMDVHIHLSYCTSKAFEALATNYLGGGAIHHPLYEEIEALVEHA 420

Query: 421 NVTPAEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGDEIVEEE 471
           NVTPAEVAEELMK +DID VMEGLAKFV+ KREEQ+  N+A  EEEG+EIVEE+
Sbjct: 421 NVTPAEVAEELMKGDDIDIVMEGLAKFVKRKREEQNDRNKA-AEEEGNEIVEEQ 471

BLAST of MS010566 vs. NCBI nr
Match: KAG6594808.1 (Phosphatidylinositol/phosphatidylcholine transfer protein SFH12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 724.5 bits (1869), Expect = 5.8e-205
Identity = 379/483 (78.47%), Postives = 420/483 (86.96%), Query Frame = 0

Query: 7   MPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQTTLVIEK 66
           MP SASSLFAAYASFATSMMMIRSITNDLLPPQL+SFISSIF Y FPPKS+PQTTLVIEK
Sbjct: 1   MPPSASSLFAAYASFATSMMMIRSITNDLLPPQLISFISSIFTYFFPPKSSPQTTLVIEK 60

Query: 67  KSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFENIHLTW 126
           K+N++KNQV+EAAEIYLRTKIS SMDRLKVSKT RQ++VSLSMEK QEI D FE+IHL W
Sbjct: 61  KTNYNKNQVFEAAEIYLRTKISPSMDRLKVSKTPRQQRVSLSMEKDQEIVDQFEDIHLKW 120

Query: 127 RFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVAKLCSQD 186
           RFV+ +KKE +E+ KEK HYELVFDKKF D ++DFY PYILRRAKEI E++NV+KLCSQ+
Sbjct: 121 RFVAEKKKEGNEFVKEKRHYELVFDKKFMDKVVDFYLPYILRRAKEITEMENVSKLCSQN 180

Query: 187 CTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEFYRKVGK 246
            +Y DD  G + CRGNWGSI LEHPATFDTLA+DPDLKKMIIDDLDRF+KRKEFYRKVGK
Sbjct: 181 LSYSDDF-GDERCRGNWGSIGLEHPATFDTLAMDPDLKKMIIDDLDRFLKRKEFYRKVGK 240

Query: 247 AWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTNRSILVI 306
           AWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDI SN  LRR+LLST+NRSILVI
Sbjct: 241 AWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDICSNSELRRSLLSTSNRSILVI 300

Query: 307 EDIDCSVNLQNR----ESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNHKE 366
           EDIDCSVNLQNR    +  ENNDD  RS+LTLSGMLNF+DGLWSSCGDERIIVLTTNHK+
Sbjct: 301 EDIDCSVNLQNRANSDDESENNDD-YRSKLTLSGMLNFMDGLWSSCGDERIIVLTTNHKD 360

Query: 367 RLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGANVTPAE 426
           RLDPALLRPGRMDVHI+LSYC+SKAF+ L TNYL G A  HPLYEEIE L++ ANVTPAE
Sbjct: 361 RLDPALLRPGRMDVHIHLSYCSSKAFEALTTNYLGGGAIHHPLYEEIEALVEHANVTPAE 420

Query: 427 VAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGD-EIVEEEQEEKLNRKKNG 485
           VAEELMK +DID VMEGLAKFV+ KREEQ+ GN A EEE  D E  E+E+   +   K  
Sbjct: 421 VAEELMKDDDIDIVMEGLAKFVKRKREEQNDGNNAAEEEGSDLESSEDERNTSIGSFKQK 480

BLAST of MS010566 vs. NCBI nr
Match: XP_022962730.1 (AAA-ATPase At2g18193-like [Cucurbita moschata])

HSP 1 Score: 708.0 bits (1826), Expect = 5.7e-200
Identity = 370/485 (76.29%), Postives = 418/485 (86.19%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQT 60
           MF+FK+MP SA+SLFAAYASFATSMMMIRSITNDLLPP+L+SFISSIF Y F  KS+PQT
Sbjct: 1   MFSFKDMPPSAASLFAAYASFATSMMMIRSITNDLLPPKLISFISSIFNYFFTLKSSPQT 60

Query: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120
           TLVI+ K+N++KNQV++AAEIYLRTKIS SMDRL  SKT RQ +VSLSM+K QEI D F+
Sbjct: 61  TLVIKNKTNYAKNQVFDAAEIYLRTKISPSMDRLAASKTPRQHRVSLSMDKDQEIVDQFQ 120

Query: 121 NIHLTWRFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180
           +IHL WRFV+ + KED ++ +EK HYEL F KKF D ++DFY PYILRRAKEIK ++NV+
Sbjct: 121 DIHLKWRFVAEKTKEDRQHIEEKRHYELSFHKKFMDKVVDFYLPYILRRAKEIKAMENVS 180

Query: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240
           KLCSQ+ +Y DD  G + CRGNWGSI LEHPATFDTLA+DPDLKKMIIDDLDRF+KRKEF
Sbjct: 181 KLCSQNLSYSDDF-GDERCRGNWGSIGLEHPATFDTLAMDPDLKKMIIDDLDRFLKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300
           YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDI SN  LRR+LLST+N
Sbjct: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDICSNSELRRSLLSTSN 300

Query: 301 RSILVIEDIDCSVNLQNR----ESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVL 360
           RSILVIEDIDCSVNLQNR    +  ENNDD  RS+LTLSGMLNF+DGLWSSCGDERIIVL
Sbjct: 301 RSILVIEDIDCSVNLQNRANSDDESENNDD-YRSKLTLSGMLNFMDGLWSSCGDERIIVL 360

Query: 361 TTNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGA 420
           TTNHK+RLDPALLRPGRMDVHI LSYCTSKAF+ L TNYL G A  HPLYEEI+ LI+ A
Sbjct: 361 TTNHKDRLDPALLRPGRMDVHIYLSYCTSKAFEALTTNYLGGGAIHHPLYEEIKALIEYA 420

Query: 421 NVTPAEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGD-EIVEEEQEEKL 480
           NVTPAEVAEELMK +DID VMEGLAKFV+LKREEQ+GGN+APE+E  + E+V   +  K 
Sbjct: 421 NVTPAEVAEELMKDDDIDIVMEGLAKFVKLKREEQNGGNKAPEKEGNEMEVVGGRRRRKW 480

BLAST of MS010566 vs. ExPASy Swiss-Prot
Match: Q8GW96 (AAA-ATPase At2g18193 OS=Arabidopsis thaliana OX=3702 GN=At2g18193 PE=2 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 1.0e-135
Identity = 248/452 (54.87%), Postives = 327/452 (72.35%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQT 60
           MF   +   S SSLF+AYAS    +M+ RS+ +D +P +L S+ SS+    F PKS    
Sbjct: 1   MFPSSDFSFSPSSLFSAYASLTGFLMLFRSMLHDFVPEKLRSYFSSLLDRFFTPKS-KYL 60

Query: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120
           T++I++    ++NQV++AAE+YLR+KI    +RL+V K  +QK  ++S+E+G+EI D FE
Sbjct: 61  TVIIDENFGLNRNQVFDAAEMYLRSKIGPETERLRVGKIPKQKHFTISIERGEEILDTFE 120

Query: 121 NIHLTWRFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180
              + W +V  E ++     K K +YEL F+KK  D +++ Y  +++  ++EIK    V 
Sbjct: 121 ESEVKWSYVQSENEKGD---KVKRYYELTFEKKLRDKVLNSYLTHVVAESEEIKRNLRVV 180

Query: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240
           KL S+D    DD  G  G  GNWG I LEHP+TFDTLA+DP+ KK IIDDL+RF+KRKEF
Sbjct: 181 KLYSRDVYASDDDDGMAG--GNWGCINLEHPSTFDTLAMDPNAKKKIIDDLERFLKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300
           Y++VGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFD++DL+L+ I  N  L+R LLSTTN
Sbjct: 241 YKRVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYDNGELKRVLLSTTN 300

Query: 301 RSILVIEDIDCSVNLQNRESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNH 360
           RSILVIEDIDC+  +++RE++   D+  + ++TLSG+LNFIDGLWSS GDERIIV TTNH
Sbjct: 301 RSILVIEDIDCNAEVRDREAENQEDEQIKGKVTLSGILNFIDGLWSSFGDERIIVFTTNH 360

Query: 361 KERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGANVTP 420
           KERLDPALLRPGRMDVHIN+SYCT   F+ L +NYL  +   HPL EEIE L+D   VTP
Sbjct: 361 KERLDPALLRPGRMDVHINMSYCTGLGFRTLVSNYLGLDGLNHPLCEEIEALVDSTEVTP 420

Query: 421 AEVAEELMKSNDIDTVMEGLAKFVRLKREEQS 453
           AE+AEELM+ +D D V+ G+  FV  ++ E+S
Sbjct: 421 AELAEELMQDDDTDVVLRGVISFVEKRKVERS 446

BLAST of MS010566 vs. ExPASy Swiss-Prot
Match: F4IQG2 (AAA-ATPase At2g18190 OS=Arabidopsis thaliana OX=3702 GN=At2g18190 PE=2 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 2.8e-125
Identity = 251/482 (52.07%), Postives = 325/482 (67.43%), Query Frame = 0

Query: 10  SASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQTTLVIEKKSN 69
           S SSLF AYAS    +M+ RS+ ND +P +L S+I+ +    F PKS    T+VI++   
Sbjct: 11  SPSSLFTAYASLTGFLMLFRSLFNDEVPERLRSYITDLLNRFFTPKS-KNLTMVIDEIIG 70

Query: 70  FSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFENIHLTWRFV 129
           F +NQV++AAE+YLR KI     RL+V K  +QK  ++ +EKG+EI D FEN  L W +V
Sbjct: 71  FKRNQVFDAAEVYLRNKIGPETARLRVGKLPKQKHFTIYIEKGEEILDTFENSELRWTYV 130

Query: 130 SVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVAKLCSQDCTY 189
              + E+    KEK +YEL F+KK  D +++ Y  +++  ++E K      KL S+D   
Sbjct: 131 ---ESENEASQKEKRYYELTFEKKLRDKVMNSYLSHVVAESEETKRDLRAVKLYSRDVRA 190

Query: 190 DDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEFYRKVGKAWK 249
             D  G  G    WG I LEHP+TF+TLA+DP  KK IIDD++RF+KR+EFY++VGKAWK
Sbjct: 191 SKDDDGMAG--AGWGCINLEHPSTFETLAMDPGAKKKIIDDMERFLKRREFYKRVGKAWK 250

Query: 250 RGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTNRSILVIEDI 309
           RGYLLYGPPGTGKSSLIAAMANYLKFD++DL+L+ I  N  L+  LLSTTNRSILVIEDI
Sbjct: 251 RGYLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYENAQLKSILLSTTNRSILVIEDI 310

Query: 310 DC-SVNLQNRESDE--NNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNHKERLDP 369
           DC S  + +RE+DE    ++    R+TLSG+LNF+DGLWSS GDERIIV TTNHKERLDP
Sbjct: 311 DCSSAEVVDREADEYQEYEEGYYGRVTLSGLLNFVDGLWSSFGDERIIVFTTNHKERLDP 370

Query: 370 ALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGANVTPAEVAEE 429
           ALLRPGRMD+HIN+SYCT   F+ L +NYL      HPL EEIE LID   VTPAE+AEE
Sbjct: 371 ALLRPGRMDMHINMSYCTGLGFRTLVSNYLGLGGLNHPLCEEIEALIDSTEVTPAELAEE 430

Query: 430 LMKSNDIDTVMEGLAKFVRLKREEQSGGNEAP----EEEEGDE---IVEEEQEEKLNRKK 482
           LM+ +D D V+ G+  FV  ++ E S   E       + +GD+   +      +K  +KK
Sbjct: 431 LMQEDDTDVVLRGVVSFVENRKVEISKTKELEGSTCRKLDGDDKHNVSSTNDLKKTKKKK 486

BLAST of MS010566 vs. ExPASy Swiss-Prot
Match: Q147F9 (AAA-ATPase At3g50940 OS=Arabidopsis thaliana OX=3702 GN=At3g50940 PE=2 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 1.1e-118
Identity = 227/449 (50.56%), Postives = 316/449 (70.38%), Query Frame = 0

Query: 10  SASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQTTLVIEKKSN 69
           +A +   A AS A + ++ RS+  D +P ++  +IS  F   F   S  Q T VIE+   
Sbjct: 11  TAKTALTAVASVAAAAILARSVVQDYMPNEVHEYISHGFRRFFSYFSY-QMTAVIEEFGG 70

Query: 70  FSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFENIHLTWRFV 129
           F  NQV+EAAE YL TKIS+S  R+KV+K  +Q   S+++E+ +E+ D F+ + L+W  V
Sbjct: 71  FEHNQVFEAAEAYLSTKISNSTRRIKVNKLEKQSNYSVTVERDEEVVDIFDGVKLSWILV 130

Query: 130 --SVEKKE-------DSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 189
              V+KK+       +S    E   YEL F KKF + +++ Y P+++ +A  IK+     
Sbjct: 131 CRHVDKKDFRNPRDLNSTLKSEVRSYELSFRKKFKNMVLESYLPFVVEQAASIKQKFKTL 190

Query: 190 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 249
           K+ + D +Y  +          W S+ L+HP+TF TLA+DP++KK +++DLDRFV+RK F
Sbjct: 191 KIFTVD-SYSVE----------WTSVTLDHPSTFRTLALDPEVKKNLVEDLDRFVQRKGF 250

Query: 250 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 309
           Y +VGKAWKRGYLLYGPPGTGKSSLIAA+AN+L FDIYDLDLT +++N  LRR L+ST N
Sbjct: 251 YGRVGKAWKRGYLLYGPPGTGKSSLIAAIANHLNFDIYDLDLTSLNNNAELRRLLMSTAN 310

Query: 310 RSILVIEDIDCSVNLQNRESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNH 369
           RSILV+EDIDCS+ L++R +D+ N+D     +TLSG+LNF+DGLWSSCG+ERIIV TTN+
Sbjct: 311 RSILVVEDIDCSIELKDRSTDQENNDPLHKTVTLSGLLNFVDGLWSSCGNERIIVFTTNY 370

Query: 370 KERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGANVTP 429
           +E+LDPALLRPGRMD+HI++SYCT  AFKVLA+NYL  E   H L+E+IE  I    VTP
Sbjct: 371 REKLDPALLRPGRMDMHIHMSYCTPAAFKVLASNYL--EIQDHILFEQIEEFIREIEVTP 430

Query: 430 AEVAEELMKSNDIDTVMEGLAKFVRLKRE 450
           AEVAE+LM+S+ +D V++GL +F++ K++
Sbjct: 431 AEVAEQLMRSDSVDKVLQGLVEFLKAKKQ 445

BLAST of MS010566 vs. ExPASy Swiss-Prot
Match: Q9FN75 (AAA-ATPase At5g17760 OS=Arabidopsis thaliana OX=3702 GN=At5g17760 PE=3 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 9.9e-115
Identity = 240/498 (48.19%), Postives = 320/498 (64.26%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQT 60
           MF  K++P S +S+F AYAS A  MMMIRS+ ++L+P  L  FI      +F   S+   
Sbjct: 1   MFFSKDLP-SPTSVFTAYASMAGYMMMIRSMAHELIPAPLQDFIYRTLRSLFFRSSSSTL 60

Query: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120
           TL I+  +    N++Y AA+ YL TKIS    RL++SK  + K V+L +  G+ + D +E
Sbjct: 61  TLTIDDDNMGMNNEIYRAAQTYLSTKISPDAVRLRISKGHKDKHVNLYLSDGEIVNDVYE 120

Query: 121 NIHLTWRFVS--VEKKED-----------------SEYAKEKYHYELVFDKKFTDTIIDF 180
           ++ L WRFV+   +KK                    +  K +Y +EL FDKK  D I++ 
Sbjct: 121 DVQLVWRFVTDGGDKKGGGGGVGGRGGGGGRRGGMDDDGKSEY-FELSFDKKHKDLILNS 180

Query: 181 YFPYILRRAKEIKELDNVAKLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDP 240
           Y PYI  +AKEI++   +  L S +                W S+ LEHP+TF+T+A++ 
Sbjct: 181 YVPYIESKAKEIRDERRILMLHSLNSL-------------RWESVILEHPSTFETMAMED 240

Query: 241 DLKKMIIDDLDRFVKRKEFYRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLD 300
           DLK+ +I+DLDRF++RKEFY++VGKAWKRGYLLYGPPGTGKSSL+AAMANYLKFD+YDL 
Sbjct: 241 DLKRDVIEDLDRFIRRKEFYKRVGKAWKRGYLLYGPPGTGKSSLVAAMANYLKFDVYDLQ 300

Query: 301 LTDIDSNYNLRRALLSTTNRSILVIEDIDCSVNLQNR---ESDENNDDSTRSRLTLSGML 360
           L  +  + +LRR LL+T NRSILVIEDIDC+V+L NR     +  N   ++  LTLSG+L
Sbjct: 301 LASVMRDSDLRRLLLATRNRSILVIEDIDCAVDLPNRIEQPVEGKNRGESQGPLTLSGLL 360

Query: 361 NFIDGLWSSCGDERIIVLTTNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNY--L 420
           NFIDGLWSSCGDERII+ TTNHK+RLDPALLRPGRMD+HI + +C+ + FK LA+NY  L
Sbjct: 361 NFIDGLWSSCGDERIIIFTTNHKDRLDPALLRPGRMDMHIYMGHCSFQGFKTLASNYLGL 420

Query: 421 SGEATAHPLYEEIEGLIDGANVTPAEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNE 475
           S  A  H L+ EIE LIDG  +TPA+VAEELMKS D D  +EGL   +   R +    N 
Sbjct: 421 SDAAMPHRLFPEIERLIDGEVMTPAQVAEELMKSEDADVALEGLVNVLEKMRLKSKESNP 480

BLAST of MS010566 vs. ExPASy Swiss-Prot
Match: Q8VZG2 (Protein HYPER-SENSITIVITY-RELATED 4 OS=Arabidopsis thaliana OX=3702 GN=HSR4 PE=2 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.8e-111
Identity = 229/495 (46.26%), Postives = 328/495 (66.26%), Query Frame = 0

Query: 10  SASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQTTLVIEKKSN 69
           +A ++    AS A + M+ RS+  D LP ++  +IS  F  IF   S+ Q T++IE+   
Sbjct: 57  TAKTVLTTAASVAATAMLARSLVQDYLPDEVHHYISYGFRSIFGYFSS-QMTIIIEEFEG 116

Query: 70  FSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFENIHLTW--R 129
           F+ N+V+EAAE YL TKIS S  R+KVSK  ++   ++++E+ +E+ D +  +   W   
Sbjct: 117 FAHNEVFEAAEAYLATKISPSNKRIKVSKHEKENNYNVTVERDEEVVDTYNGVKFQWILH 176

Query: 130 FVSVEKKE-------DSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 189
              VE K        +S    E   +EL F KKF D  ++ Y P++++RA  +K+     
Sbjct: 177 CRHVESKHFHNPRDLNSTLRSEVRSFELNFHKKFKDVALESYLPFMVKRATLMKQEKKTL 236

Query: 190 KL--CSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRK 249
           K+   S +  Y + S         W S+ L+HP+TF TLA+D D+K  +++DLD+FVKR+
Sbjct: 237 KIFTLSPENMYGNYSDA-------WTSVTLDHPSTFKTLAMDSDVKTSVMEDLDKFVKRR 296

Query: 250 EFYRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLST 309
           +FY++VGKAWKRGYLLYGPPGTGKSSLIAAMAN+L FDIYDL+LT +++N  LRR L++T
Sbjct: 297 DFYKRVGKAWKRGYLLYGPPGTGKSSLIAAMANHLNFDIYDLELTAVNNNSELRRLLIAT 356

Query: 310 TNRSILVIEDIDCSVNLQNRESDE------NNDDSTRSRLTLSGMLNFIDGLWSSCGDER 369
            NRSIL++EDIDCS+ L++R SDE      + +D    ++TLSG+LNFIDGLWSSCGDER
Sbjct: 357 ANRSILIVEDIDCSLELKDRTSDEPPRESDDIEDPRYKKVTLSGLLNFIDGLWSSCGDER 416

Query: 370 IIVLTTNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGL 429
           II+ TTN+KE+LD ALLRPGRMD+HI++SYCT   FK LA NYL  E   H L+ +IE  
Sbjct: 417 IIIFTTNYKEKLDAALLRPGRMDMHIHMSYCTPSTFKALALNYL--EIKEHRLFSKIEEG 476

Query: 430 IDGANVTPAEVAEELMKSNDIDTVMEGLAKFVRLKR----------EEQSGGNEAPEEEE 478
           I+   VTPAEVAE+LM+++ +D V+EGL +F+++K+          E+Q   N+   +E 
Sbjct: 477 IEATEVTPAEVAEQLMRNDSVDKVLEGLIEFLKVKKIENEQDKAKTEKQELENKKKTKEG 536

BLAST of MS010566 vs. ExPASy TrEMBL
Match: A0A6J1BTY0 (AAA-ATPase At2g18193-like OS=Momordica charantia OX=3673 GN=LOC111005660 PE=3 SV=1)

HSP 1 Score: 933.7 bits (2412), Expect = 3.1e-268
Identity = 478/484 (98.76%), Postives = 479/484 (98.97%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQT 60
           MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSI FY FPPKS+PQ 
Sbjct: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSILFYFFPPKSSPQA 60

Query: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120
           TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE
Sbjct: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120

Query: 121 NIHLTWRFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180
           NIHLTWRFVSVEKKED EYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA
Sbjct: 121 NIHLTWRFVSVEKKEDGEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180

Query: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240
           KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF
Sbjct: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300
           YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN
Sbjct: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300

Query: 301 RSILVIEDIDCSVNLQNRESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNH 360
           RSILVIEDIDCSVNLQNRESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNH
Sbjct: 301 RSILVIEDIDCSVNLQNRESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNH 360

Query: 361 KERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGANVTP 420
           KERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYL GEATAHPLYEEIEGLIDGANVTP
Sbjct: 361 KERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLGGEATAHPLYEEIEGLIDGANVTP 420

Query: 421 AEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGDEIVEEEQEEKLNRKKN 480
           AEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGDEIVEEEQEEKLNRKKN
Sbjct: 421 AEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGDEIVEEEQEEKLNRKKN 480

Query: 481 GAGV 485
           GAGV
Sbjct: 481 GAGV 484

BLAST of MS010566 vs. ExPASy TrEMBL
Match: A0A6J1HFS7 (AAA-ATPase At2g18193-like OS=Cucurbita moschata OX=3662 GN=LOC111463099 PE=3 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 4.2e-209
Identity = 383/474 (80.80%), Postives = 422/474 (89.03%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQT 60
           M  FKEMP SASSLFAAYASFATSMMMIRSITNDLLPPQL+SFISSIF Y FPPKS+PQT
Sbjct: 1   MLNFKEMPPSASSLFAAYASFATSMMMIRSITNDLLPPQLISFISSIFTYFFPPKSSPQT 60

Query: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120
           TLVIEKK+N++KNQV+EAAEIYLRTKIS SMDRLKVSKT RQ++VSLSMEK QEI D FE
Sbjct: 61  TLVIEKKTNYNKNQVFEAAEIYLRTKISPSMDRLKVSKTPRQQRVSLSMEKDQEIVDQFE 120

Query: 121 NIHLTWRFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180
           +IHL WRFV+ +KKE +E+ KEK HYELVFDKKF D ++DFY PYILRRAKEI E++NV+
Sbjct: 121 DIHLKWRFVAEKKKEGNEFVKEKRHYELVFDKKFMDKVVDFYLPYILRRAKEITEMENVS 180

Query: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240
           KLCSQ+ +Y DD  G + CRGNWGSI LEHPATFDTLA+DPDLKKMIIDDLDRF+KRKEF
Sbjct: 181 KLCSQNLSYSDDF-GDERCRGNWGSIGLEHPATFDTLAMDPDLKKMIIDDLDRFLKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300
           YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDI SN  LRR+LLST+N
Sbjct: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDICSNSELRRSLLSTSN 300

Query: 301 RSILVIEDIDCSVNLQNR----ESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVL 360
           RSILVIEDIDCSVNLQNR    +  ENNDD  RS+LTLSGMLNF+DGLWSSCGDERIIVL
Sbjct: 301 RSILVIEDIDCSVNLQNRANSDDESENNDD-YRSKLTLSGMLNFMDGLWSSCGDERIIVL 360

Query: 361 TTNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGA 420
           TTNHK+RLDPALLRPGRMDVHI+LSYC+SKAF+ L TNYL G A  HPLYEEIE L++ A
Sbjct: 361 TTNHKDRLDPALLRPGRMDVHIHLSYCSSKAFEALTTNYLGGGAIHHPLYEEIEALVEHA 420

Query: 421 NVTPAEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGDEIVEEE 471
           NVTPAEVAEELMK +DID VMEGLAKFV+ KREEQ+ GN A  EEEG+EIVEE+
Sbjct: 421 NVTPAEVAEELMKDDDIDIVMEGLAKFVKRKREEQNDGNNA-AEEEGNEIVEEQ 471

BLAST of MS010566 vs. ExPASy TrEMBL
Match: A0A6J1HFX6 (AAA-ATPase At2g18193-like OS=Cucurbita moschata OX=3662 GN=LOC111463134 PE=3 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.7e-200
Identity = 370/485 (76.29%), Postives = 418/485 (86.19%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQT 60
           MF+FK+MP SA+SLFAAYASFATSMMMIRSITNDLLPP+L+SFISSIF Y F  KS+PQT
Sbjct: 1   MFSFKDMPPSAASLFAAYASFATSMMMIRSITNDLLPPKLISFISSIFNYFFTLKSSPQT 60

Query: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120
           TLVI+ K+N++KNQV++AAEIYLRTKIS SMDRL  SKT RQ +VSLSM+K QEI D F+
Sbjct: 61  TLVIKNKTNYAKNQVFDAAEIYLRTKISPSMDRLAASKTPRQHRVSLSMDKDQEIVDQFQ 120

Query: 121 NIHLTWRFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180
           +IHL WRFV+ + KED ++ +EK HYEL F KKF D ++DFY PYILRRAKEIK ++NV+
Sbjct: 121 DIHLKWRFVAEKTKEDRQHIEEKRHYELSFHKKFMDKVVDFYLPYILRRAKEIKAMENVS 180

Query: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240
           KLCSQ+ +Y DD  G + CRGNWGSI LEHPATFDTLA+DPDLKKMIIDDLDRF+KRKEF
Sbjct: 181 KLCSQNLSYSDDF-GDERCRGNWGSIGLEHPATFDTLAMDPDLKKMIIDDLDRFLKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300
           YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDI SN  LRR+LLST+N
Sbjct: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDICSNSELRRSLLSTSN 300

Query: 301 RSILVIEDIDCSVNLQNR----ESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVL 360
           RSILVIEDIDCSVNLQNR    +  ENNDD  RS+LTLSGMLNF+DGLWSSCGDERIIVL
Sbjct: 301 RSILVIEDIDCSVNLQNRANSDDESENNDD-YRSKLTLSGMLNFMDGLWSSCGDERIIVL 360

Query: 361 TTNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGA 420
           TTNHK+RLDPALLRPGRMDVHI LSYCTSKAF+ L TNYL G A  HPLYEEI+ LI+ A
Sbjct: 361 TTNHKDRLDPALLRPGRMDVHIYLSYCTSKAFEALTTNYLGGGAIHHPLYEEIKALIEYA 420

Query: 421 NVTPAEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGD-EIVEEEQEEKL 480
           NVTPAEVAEELMK +DID VMEGLAKFV+LKREEQ+GGN+APE+E  + E+V   +  K 
Sbjct: 421 NVTPAEVAEELMKDDDIDIVMEGLAKFVKLKREEQNGGNKAPEKEGNEMEVVGGRRRRKW 480

BLAST of MS010566 vs. ExPASy TrEMBL
Match: A0A6J1KNM3 (AAA-ATPase At2g18193-like OS=Cucurbita maxima OX=3661 GN=LOC111497272 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.7e-194
Identity = 363/474 (76.58%), Postives = 411/474 (86.71%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATS-MMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQ 60
           MF+FK+MP SASSLFAAYASFATS MMMIRSITNDLLPP+L+SFISSIF Y F PKS+P 
Sbjct: 1   MFSFKDMPPSASSLFAAYASFATSMMMMIRSITNDLLPPKLVSFISSIFNYFFTPKSSPH 60

Query: 61  TTLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDF 120
           TTLVI+ K+N++KNQV++AAEIYLRTKIS SMDRL+ SKT RQ +VSLSM+K QEI D F
Sbjct: 61  TTLVIKNKTNYAKNQVFDAAEIYLRTKISPSMDRLEASKTPRQHRVSLSMDKDQEIVDQF 120

Query: 121 ENIHLTWRFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNV 180
           ++IHL WRFV+ +KKED ++ +EK   +LVF KKF D ++DFY PYILRRAKEIK  +NV
Sbjct: 121 QDIHLKWRFVAEKKKEDRQHIEEK--SQLVFHKKFVDKVVDFYLPYILRRAKEIKATENV 180

Query: 181 AKLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKE 240
           ++LCSQ+  Y DDS G D CRG+WGSI L HPATFDTLA+DPDLKKMIID+LDRF+KRKE
Sbjct: 181 SELCSQNLPYSDDS-GEDRCRGHWGSIGLGHPATFDTLAMDPDLKKMIIDELDRFLKRKE 240

Query: 241 FYRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTT 300
           FYRKVGKAWKRGYLLYGPPGTGKSSL+AAMANYLKFDIYDLDLTDI SN +LRR+LLST+
Sbjct: 241 FYRKVGKAWKRGYLLYGPPGTGKSSLVAAMANYLKFDIYDLDLTDICSNSDLRRSLLSTS 300

Query: 301 NRSILVIEDIDCSVNLQNR----ESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIV 360
           NRSILVIEDIDCSVNLQNR    +  ENNDD  RS+L LSGMLNF+DGLWSSCGDERIIV
Sbjct: 301 NRSILVIEDIDCSVNLQNRANSDDESENNDD-YRSKLMLSGMLNFMDGLWSSCGDERIIV 360

Query: 361 LTTNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDG 420
           LTTNHK+RLD ALLRPGRMDVHINLSYCTSKAF+ L TNYL G A  HPLYEEIE L++ 
Sbjct: 361 LTTNHKDRLDAALLRPGRMDVHINLSYCTSKAFETLVTNYLGGGAIHHPLYEEIEALVEH 420

Query: 421 ANVTPAEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGDEIVEE 470
           ANVTPAEVAEELMK +DID +MEGLAKFV+ KREEQ+ GN A EEE  +EIVEE
Sbjct: 421 ANVTPAEVAEELMKDDDIDILMEGLAKFVKRKREEQNDGNMAAEEEV-NEIVEE 469

BLAST of MS010566 vs. ExPASy TrEMBL
Match: A0A1S3B1F9 (AAA-ATPase At2g18193-like OS=Cucumis melo OX=3656 GN=LOC103485084 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 2.0e-187
Identity = 341/474 (71.94%), Postives = 400/474 (84.39%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQT 60
           MF FK+MPQSASSLF AYASFAT+ MMIRS+T  LLPPQL+S ISS+ FY FPPKST  T
Sbjct: 1   MFGFKDMPQSASSLFTAYASFATTAMMIRSMTTTLLPPQLISLISSVIFYFFPPKSTLIT 60

Query: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120
           T+VI++K +F  NQ++EAA++YLRTKI+ SMDRLK SKT RQ KV+LSMEKGQ+I D FE
Sbjct: 61  TIVIDQKCDFLNNQLFEAAQLYLRTKINPSMDRLKASKTPRQNKVALSMEKGQKIVDHFE 120

Query: 121 NIHLTWRFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180
           +I L W FV+ +K++ +EY++EK HYELVF KKF D +++FYFPYIL+RAKEIK LDNVA
Sbjct: 121 DIRLQWGFVATQKEKRNEYSEEKCHYELVFPKKFLDRVVNFYFPYILQRAKEIKALDNVA 180

Query: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240
           KLCS  C+YDD+S G    +G WGS+ LEHPATFDTLA+DPDLKKMIIDDLDRFVKRKEF
Sbjct: 181 KLCSSSCSYDDESLGGKR-QGKWGSVRLEHPATFDTLALDPDLKKMIIDDLDRFVKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300
           YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDL+D+ SN +LR +LLSTTN
Sbjct: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSDVYSNQSLRSSLLSTTN 300

Query: 301 RSILVIEDIDCSVNLQNRESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNH 360
           RSILVIEDIDCSV+LQNR+ +E   +  +SRLTLSGMLNFIDGLWSSCGDERII+ TTNH
Sbjct: 301 RSILVIEDIDCSVDLQNRKFEE-KFEPPKSRLTLSGMLNFIDGLWSSCGDERIIIFTTNH 360

Query: 361 KERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGANVTP 420
           KE+LDPALLRPGRMDVHI+L YCTSK  KVLATNYL  E T H LYEE++GLID  N TP
Sbjct: 361 KEKLDPALLRPGRMDVHIHLGYCTSKMLKVLATNYLGAEVTDHRLYEEVQGLIDCVNATP 420

Query: 421 AEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNEAPEEEEGDEIVEEEQEEK 475
           AE+AEELMKS+DID V+EGLA F++LK +E+  G     +E+ D I+EE +  +
Sbjct: 421 AEIAEELMKSDDIDVVIEGLANFLKLKSKERKAG-----DEKIDRILEENKASR 467

BLAST of MS010566 vs. TAIR 10
Match: AT2G18193.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 485.0 bits (1247), Expect = 7.3e-137
Identity = 248/452 (54.87%), Postives = 327/452 (72.35%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQT 60
           MF   +   S SSLF+AYAS    +M+ RS+ +D +P +L S+ SS+    F PKS    
Sbjct: 1   MFPSSDFSFSPSSLFSAYASLTGFLMLFRSMLHDFVPEKLRSYFSSLLDRFFTPKS-KYL 60

Query: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120
           T++I++    ++NQV++AAE+YLR+KI    +RL+V K  +QK  ++S+E+G+EI D FE
Sbjct: 61  TVIIDENFGLNRNQVFDAAEMYLRSKIGPETERLRVGKIPKQKHFTISIERGEEILDTFE 120

Query: 121 NIHLTWRFVSVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 180
              + W +V  E ++     K K +YEL F+KK  D +++ Y  +++  ++EIK    V 
Sbjct: 121 ESEVKWSYVQSENEKGD---KVKRYYELTFEKKLRDKVLNSYLTHVVAESEEIKRNLRVV 180

Query: 181 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 240
           KL S+D    DD  G  G  GNWG I LEHP+TFDTLA+DP+ KK IIDDL+RF+KRKEF
Sbjct: 181 KLYSRDVYASDDDDGMAG--GNWGCINLEHPSTFDTLAMDPNAKKKIIDDLERFLKRKEF 240

Query: 241 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 300
           Y++VGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFD++DL+L+ I  N  L+R LLSTTN
Sbjct: 241 YKRVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYDNGELKRVLLSTTN 300

Query: 301 RSILVIEDIDCSVNLQNRESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNH 360
           RSILVIEDIDC+  +++RE++   D+  + ++TLSG+LNFIDGLWSS GDERIIV TTNH
Sbjct: 301 RSILVIEDIDCNAEVRDREAENQEDEQIKGKVTLSGILNFIDGLWSSFGDERIIVFTTNH 360

Query: 361 KERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGANVTP 420
           KERLDPALLRPGRMDVHIN+SYCT   F+ L +NYL  +   HPL EEIE L+D   VTP
Sbjct: 361 KERLDPALLRPGRMDVHINMSYCTGLGFRTLVSNYLGLDGLNHPLCEEIEALVDSTEVTP 420

Query: 421 AEVAEELMKSNDIDTVMEGLAKFVRLKREEQS 453
           AE+AEELM+ +D D V+ G+  FV  ++ E+S
Sbjct: 421 AELAEELMQDDDTDVVLRGVISFVEKRKVERS 446

BLAST of MS010566 vs. TAIR 10
Match: AT2G18190.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 450.3 bits (1157), Expect = 2.0e-126
Identity = 251/482 (52.07%), Postives = 325/482 (67.43%), Query Frame = 0

Query: 10  SASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQTTLVIEKKSN 69
           S SSLF AYAS    +M+ RS+ ND +P +L S+I+ +    F PKS    T+VI++   
Sbjct: 11  SPSSLFTAYASLTGFLMLFRSLFNDEVPERLRSYITDLLNRFFTPKS-KNLTMVIDEIIG 70

Query: 70  FSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFENIHLTWRFV 129
           F +NQV++AAE+YLR KI     RL+V K  +QK  ++ +EKG+EI D FEN  L W +V
Sbjct: 71  FKRNQVFDAAEVYLRNKIGPETARLRVGKLPKQKHFTIYIEKGEEILDTFENSELRWTYV 130

Query: 130 SVEKKEDSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVAKLCSQDCTY 189
              + E+    KEK +YEL F+KK  D +++ Y  +++  ++E K      KL S+D   
Sbjct: 131 ---ESENEASQKEKRYYELTFEKKLRDKVMNSYLSHVVAESEETKRDLRAVKLYSRDVRA 190

Query: 190 DDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEFYRKVGKAWK 249
             D  G  G    WG I LEHP+TF+TLA+DP  KK IIDD++RF+KR+EFY++VGKAWK
Sbjct: 191 SKDDDGMAG--AGWGCINLEHPSTFETLAMDPGAKKKIIDDMERFLKRREFYKRVGKAWK 250

Query: 250 RGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTNRSILVIEDI 309
           RGYLLYGPPGTGKSSLIAAMANYLKFD++DL+L+ I  N  L+  LLSTTNRSILVIEDI
Sbjct: 251 RGYLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYENAQLKSILLSTTNRSILVIEDI 310

Query: 310 DC-SVNLQNRESDE--NNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNHKERLDP 369
           DC S  + +RE+DE    ++    R+TLSG+LNF+DGLWSS GDERIIV TTNHKERLDP
Sbjct: 311 DCSSAEVVDREADEYQEYEEGYYGRVTLSGLLNFVDGLWSSFGDERIIVFTTNHKERLDP 370

Query: 370 ALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGANVTPAEVAEE 429
           ALLRPGRMD+HIN+SYCT   F+ L +NYL      HPL EEIE LID   VTPAE+AEE
Sbjct: 371 ALLRPGRMDMHINMSYCTGLGFRTLVSNYLGLGGLNHPLCEEIEALIDSTEVTPAELAEE 430

Query: 430 LMKSNDIDTVMEGLAKFVRLKREEQSGGNEAP----EEEEGDE---IVEEEQEEKLNRKK 482
           LM+ +D D V+ G+  FV  ++ E S   E       + +GD+   +      +K  +KK
Sbjct: 431 LMQEDDTDVVLRGVVSFVENRKVEISKTKELEGSTCRKLDGDDKHNVSSTNDLKKTKKKK 486

BLAST of MS010566 vs. TAIR 10
Match: AT3G50940.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 428.3 bits (1100), Expect = 8.1e-120
Identity = 227/449 (50.56%), Postives = 316/449 (70.38%), Query Frame = 0

Query: 10  SASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQTTLVIEKKSN 69
           +A +   A AS A + ++ RS+  D +P ++  +IS  F   F   S  Q T VIE+   
Sbjct: 11  TAKTALTAVASVAAAAILARSVVQDYMPNEVHEYISHGFRRFFSYFSY-QMTAVIEEFGG 70

Query: 70  FSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFENIHLTWRFV 129
           F  NQV+EAAE YL TKIS+S  R+KV+K  +Q   S+++E+ +E+ D F+ + L+W  V
Sbjct: 71  FEHNQVFEAAEAYLSTKISNSTRRIKVNKLEKQSNYSVTVERDEEVVDIFDGVKLSWILV 130

Query: 130 --SVEKKE-------DSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 189
              V+KK+       +S    E   YEL F KKF + +++ Y P+++ +A  IK+     
Sbjct: 131 CRHVDKKDFRNPRDLNSTLKSEVRSYELSFRKKFKNMVLESYLPFVVEQAASIKQKFKTL 190

Query: 190 KLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRKEF 249
           K+ + D +Y  +          W S+ L+HP+TF TLA+DP++KK +++DLDRFV+RK F
Sbjct: 191 KIFTVD-SYSVE----------WTSVTLDHPSTFRTLALDPEVKKNLVEDLDRFVQRKGF 250

Query: 250 YRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLSTTN 309
           Y +VGKAWKRGYLLYGPPGTGKSSLIAA+AN+L FDIYDLDLT +++N  LRR L+ST N
Sbjct: 251 YGRVGKAWKRGYLLYGPPGTGKSSLIAAIANHLNFDIYDLDLTSLNNNAELRRLLMSTAN 310

Query: 310 RSILVIEDIDCSVNLQNRESDENNDDSTRSRLTLSGMLNFIDGLWSSCGDERIIVLTTNH 369
           RSILV+EDIDCS+ L++R +D+ N+D     +TLSG+LNF+DGLWSSCG+ERIIV TTN+
Sbjct: 311 RSILVVEDIDCSIELKDRSTDQENNDPLHKTVTLSGLLNFVDGLWSSCGNERIIVFTTNY 370

Query: 370 KERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGLIDGANVTP 429
           +E+LDPALLRPGRMD+HI++SYCT  AFKVLA+NYL  E   H L+E+IE  I    VTP
Sbjct: 371 REKLDPALLRPGRMDMHIHMSYCTPAAFKVLASNYL--EIQDHILFEQIEEFIREIEVTP 430

Query: 430 AEVAEELMKSNDIDTVMEGLAKFVRLKRE 450
           AEVAE+LM+S+ +D V++GL +F++ K++
Sbjct: 431 AEVAEQLMRSDSVDKVLQGLVEFLKAKKQ 445

BLAST of MS010566 vs. TAIR 10
Match: AT5G17760.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 415.2 bits (1066), Expect = 7.1e-116
Identity = 240/498 (48.19%), Postives = 320/498 (64.26%), Query Frame = 0

Query: 1   MFAFKEMPQSASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQT 60
           MF  K++P S +S+F AYAS A  MMMIRS+ ++L+P  L  FI      +F   S+   
Sbjct: 1   MFFSKDLP-SPTSVFTAYASMAGYMMMIRSMAHELIPAPLQDFIYRTLRSLFFRSSSSTL 60

Query: 61  TLVIEKKSNFSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFE 120
           TL I+  +    N++Y AA+ YL TKIS    RL++SK  + K V+L +  G+ + D +E
Sbjct: 61  TLTIDDDNMGMNNEIYRAAQTYLSTKISPDAVRLRISKGHKDKHVNLYLSDGEIVNDVYE 120

Query: 121 NIHLTWRFVS--VEKKED-----------------SEYAKEKYHYELVFDKKFTDTIIDF 180
           ++ L WRFV+   +KK                    +  K +Y +EL FDKK  D I++ 
Sbjct: 121 DVQLVWRFVTDGGDKKGGGGGVGGRGGGGGRRGGMDDDGKSEY-FELSFDKKHKDLILNS 180

Query: 181 YFPYILRRAKEIKELDNVAKLCSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDP 240
           Y PYI  +AKEI++   +  L S +                W S+ LEHP+TF+T+A++ 
Sbjct: 181 YVPYIESKAKEIRDERRILMLHSLNSL-------------RWESVILEHPSTFETMAMED 240

Query: 241 DLKKMIIDDLDRFVKRKEFYRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLD 300
           DLK+ +I+DLDRF++RKEFY++VGKAWKRGYLLYGPPGTGKSSL+AAMANYLKFD+YDL 
Sbjct: 241 DLKRDVIEDLDRFIRRKEFYKRVGKAWKRGYLLYGPPGTGKSSLVAAMANYLKFDVYDLQ 300

Query: 301 LTDIDSNYNLRRALLSTTNRSILVIEDIDCSVNLQNR---ESDENNDDSTRSRLTLSGML 360
           L  +  + +LRR LL+T NRSILVIEDIDC+V+L NR     +  N   ++  LTLSG+L
Sbjct: 301 LASVMRDSDLRRLLLATRNRSILVIEDIDCAVDLPNRIEQPVEGKNRGESQGPLTLSGLL 360

Query: 361 NFIDGLWSSCGDERIIVLTTNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNY--L 420
           NFIDGLWSSCGDERII+ TTNHK+RLDPALLRPGRMD+HI + +C+ + FK LA+NY  L
Sbjct: 361 NFIDGLWSSCGDERIIIFTTNHKDRLDPALLRPGRMDMHIYMGHCSFQGFKTLASNYLGL 420

Query: 421 SGEATAHPLYEEIEGLIDGANVTPAEVAEELMKSNDIDTVMEGLAKFVRLKREEQSGGNE 475
           S  A  H L+ EIE LIDG  +TPA+VAEELMKS D D  +EGL   +   R +    N 
Sbjct: 421 SDAAMPHRLFPEIERLIDGEVMTPAQVAEELMKSEDADVALEGLVNVLEKMRLKSKESNP 480

BLAST of MS010566 vs. TAIR 10
Match: AT3G50930.1 (cytochrome BC1 synthesis )

HSP 1 Score: 404.4 bits (1038), Expect = 1.2e-112
Identity = 229/495 (46.26%), Postives = 328/495 (66.26%), Query Frame = 0

Query: 10  SASSLFAAYASFATSMMMIRSITNDLLPPQLLSFISSIFFYIFPPKSTPQTTLVIEKKSN 69
           +A ++    AS A + M+ RS+  D LP ++  +IS  F  IF   S+ Q T++IE+   
Sbjct: 57  TAKTVLTTAASVAATAMLARSLVQDYLPDEVHHYISYGFRSIFGYFSS-QMTIIIEEFEG 116

Query: 70  FSKNQVYEAAEIYLRTKISSSMDRLKVSKTGRQKKVSLSMEKGQEITDDFENIHLTW--R 129
           F+ N+V+EAAE YL TKIS S  R+KVSK  ++   ++++E+ +E+ D +  +   W   
Sbjct: 117 FAHNEVFEAAEAYLATKISPSNKRIKVSKHEKENNYNVTVERDEEVVDTYNGVKFQWILH 176

Query: 130 FVSVEKKE-------DSEYAKEKYHYELVFDKKFTDTIIDFYFPYILRRAKEIKELDNVA 189
              VE K        +S    E   +EL F KKF D  ++ Y P++++RA  +K+     
Sbjct: 177 CRHVESKHFHNPRDLNSTLRSEVRSFELNFHKKFKDVALESYLPFMVKRATLMKQEKKTL 236

Query: 190 KL--CSQDCTYDDDSGGSDGCRGNWGSIALEHPATFDTLAVDPDLKKMIIDDLDRFVKRK 249
           K+   S +  Y + S         W S+ L+HP+TF TLA+D D+K  +++DLD+FVKR+
Sbjct: 237 KIFTLSPENMYGNYSDA-------WTSVTLDHPSTFKTLAMDSDVKTSVMEDLDKFVKRR 296

Query: 250 EFYRKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTDIDSNYNLRRALLST 309
           +FY++VGKAWKRGYLLYGPPGTGKSSLIAAMAN+L FDIYDL+LT +++N  LRR L++T
Sbjct: 297 DFYKRVGKAWKRGYLLYGPPGTGKSSLIAAMANHLNFDIYDLELTAVNNNSELRRLLIAT 356

Query: 310 TNRSILVIEDIDCSVNLQNRESDE------NNDDSTRSRLTLSGMLNFIDGLWSSCGDER 369
            NRSIL++EDIDCS+ L++R SDE      + +D    ++TLSG+LNFIDGLWSSCGDER
Sbjct: 357 ANRSILIVEDIDCSLELKDRTSDEPPRESDDIEDPRYKKVTLSGLLNFIDGLWSSCGDER 416

Query: 370 IIVLTTNHKERLDPALLRPGRMDVHINLSYCTSKAFKVLATNYLSGEATAHPLYEEIEGL 429
           II+ TTN+KE+LD ALLRPGRMD+HI++SYCT   FK LA NYL  E   H L+ +IE  
Sbjct: 417 IIIFTTNYKEKLDAALLRPGRMDMHIHMSYCTPSTFKALALNYL--EIKEHRLFSKIEEG 476

Query: 430 IDGANVTPAEVAEELMKSNDIDTVMEGLAKFVRLKR----------EEQSGGNEAPEEEE 478
           I+   VTPAEVAE+LM+++ +D V+EGL +F+++K+          E+Q   N+   +E 
Sbjct: 477 IEATEVTPAEVAEQLMRNDSVDKVLEGLIEFLKVKKIENEQDKAKTEKQELENKKKTKEG 536

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132940.16.3e-26898.76AAA-ATPase At2g18193-like [Momordica charantia][more]
XP_022962680.18.7e-20980.80AAA-ATPase At2g18193-like [Cucurbita moschata][more]
XP_023517746.11.5e-20880.59AAA-ATPase At2g18193-like [Cucurbita pepo subsp. pepo][more]
KAG6594808.15.8e-20578.47Phosphatidylinositol/phosphatidylcholine transfer protein SFH12, partial [Cucurb... [more]
XP_022962730.15.7e-20076.29AAA-ATPase At2g18193-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8GW961.0e-13554.87AAA-ATPase At2g18193 OS=Arabidopsis thaliana OX=3702 GN=At2g18193 PE=2 SV=1[more]
F4IQG22.8e-12552.07AAA-ATPase At2g18190 OS=Arabidopsis thaliana OX=3702 GN=At2g18190 PE=2 SV=1[more]
Q147F91.1e-11850.56AAA-ATPase At3g50940 OS=Arabidopsis thaliana OX=3702 GN=At3g50940 PE=2 SV=1[more]
Q9FN759.9e-11548.19AAA-ATPase At5g17760 OS=Arabidopsis thaliana OX=3702 GN=At5g17760 PE=3 SV=1[more]
Q8VZG21.8e-11146.26Protein HYPER-SENSITIVITY-RELATED 4 OS=Arabidopsis thaliana OX=3702 GN=HSR4 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1BTY03.1e-26898.76AAA-ATPase At2g18193-like OS=Momordica charantia OX=3673 GN=LOC111005660 PE=3 SV... [more]
A0A6J1HFS74.2e-20980.80AAA-ATPase At2g18193-like OS=Cucurbita moschata OX=3662 GN=LOC111463099 PE=3 SV=... [more]
A0A6J1HFX62.7e-20076.29AAA-ATPase At2g18193-like OS=Cucurbita moschata OX=3662 GN=LOC111463134 PE=3 SV=... [more]
A0A6J1KNM31.7e-19476.58AAA-ATPase At2g18193-like OS=Cucurbita maxima OX=3661 GN=LOC111497272 PE=3 SV=1[more]
A0A1S3B1F92.0e-18771.94AAA-ATPase At2g18193-like OS=Cucumis melo OX=3656 GN=LOC103485084 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G18193.17.3e-13754.87P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G18190.12.0e-12652.07P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G50940.18.1e-12050.56P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G17760.17.1e-11648.19P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G50930.11.2e-11246.26cytochrome BC1 synthesis [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 248..384
e-value: 4.6E-7
score: 39.5
IPR025753AAA-type ATPase, N-terminal domainPFAMPF14363AAA_assoccoord: 36..129
e-value: 1.1E-21
score: 76.9
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 253..381
e-value: 3.1E-19
score: 69.7
NoneNo IPR availableGENE3D6.10.280.40coord: 379..448
e-value: 5.6E-18
score: 66.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 448..484
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 455..469
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 470..484
NoneNo IPR availablePANTHERPTHR23070:SF160SUBFAMILY NOT NAMEDcoord: 4..454
NoneNo IPR availablePANTHERPTHR23070BCS1 AAA-TYPE ATPASEcoord: 4..454
NoneNo IPR availableCDDcd00009AAAcoord: 249..382
e-value: 8.93832E-13
score: 64.0895
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 205..378
e-value: 1.0E-44
score: 154.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 205..429
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 352..370

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010566.1MS010566.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity