MS010539 (gene) Bitter gourd (TR) v1

BLAST of MS010539 vs. NCBI nr
Match: XP_022132422.1 (pentatricopeptide repeat-containing protein At5g66520 [Momordica charantia])

HSP 1 Score: 1008.8 bits (2607), Expect = 1.6e-290
Identity = 500/539 (92.76%), Postives = 502/539 (93.14%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WNAIIRAYSDSNEPKIAFLLYCQM+SSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV
Sbjct: 78  WNAIIRAYSDSNEPKIAFLLYCQMLSSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 137

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERD---------------------- 120
           KLGFGSDVFAMNSLIRAY LCGDIQYARQLFDHIPERD                      
Sbjct: 138 KLGFGSDVFAMNSLIRAYTLCGDIQYARQLFDHIPERDVVSWNTMVDGYIKFGDVKSAYG 197

Query: 121 --------NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDGVAVASMLTACANLGA 180
                   NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELD VAVASMLTACANLGA
Sbjct: 198 VFLEMPLKNVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDAVAVASMLTACANLGA 257

Query: 181 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 240
           LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI
Sbjct: 258 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 317

Query: 241 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 300
           EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL
Sbjct: 318 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 377

Query: 301 SPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 360
           SPCIEHYGCMVDLLGRAGLLQE KELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT
Sbjct: 378 SPCIEHYGCMVDLLGRAGLLQEAKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 437

Query: 361 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEFL 420
           HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMK LRVLIPPGKSLITLDGVVHEFL
Sbjct: 438 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKKLRVLIPPGKSLITLDGVVHEFL 497

Query: 421 AGHQDHPQMKQIHQKLNQVAERLHQEGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAFG 480
           AGHQDHPQMKQIHQKLNQVAERL +EGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAFG
Sbjct: 498 AGHQDHPQMKQIHQKLNQVAERLRKEGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAFG 557

Query: 481 LINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           LIN KPGTTIRVVKNLRVCGDCH VAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW
Sbjct: 558 LINMKPGTTIRVVKNLRVCGDCHDVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 616

BLAST of MS010539 vs. NCBI nr
Match: XP_022978438.1 (pentatricopeptide repeat-containing protein At5g66520 [Cucurbita maxima])

HSP 1 Score: 855.1 bits (2208), Expect = 3.0e-244
Identity = 418/540 (77.41%), Postives = 464/540 (85.93%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WNA+IRAYS+SNEP++AFLLY QM+SSSVP NS+TFPFLLK+CR+ SAM EALQVHGL++
Sbjct: 82  WNAMIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFLLKACRNFSAMSEALQVHGLVI 141

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERD---------------------- 120
           KLGFGSDVFA+N+L+  YALCGDIQYARQLFD+IPERD                      
Sbjct: 142 KLGFGSDVFALNALLHVYALCGDIQYARQLFDNIPERDIVSWNIMIDGYIKSGDVKTAYG 201

Query: 121 --------NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDGVAVASMLTACANLGA 180
                   NVVSWTS+ISGLVEAGL VEAL LC EMQ AGFELDGVA+AS+LTACANLGA
Sbjct: 202 VFLDMPLKNVVSWTSLISGLVEAGLNVEALSLCHEMQNAGFELDGVAIASLLTACANLGA 261

Query: 181 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 240
           L+QGRWLHFYVLNNGVH+DRVI CALVNMY+KCGD+EEALQ F +LK   KDV+VWTAMI
Sbjct: 262 LDQGRWLHFYVLNNGVHVDRVIGCALVNMYLKCGDMEEALQEFGKLKGDQKDVYVWTAMI 321

Query: 241 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 300
           +GFAI+GRGVEALEWF +M REG RPNSITFTA+L ACSY GLVEEGK LF+SM S+Y L
Sbjct: 322 DGFAIHGRGVEALEWFKRMLREGIRPNSITFTAVLRACSYAGLVEEGKVLFESMMSVYIL 381

Query: 301 SPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 360
           SP IEHYGCMVDLLGRAGLL+E KELI+ MPMKPNA+IWGALLKAC+IH DF + GQIG 
Sbjct: 382 SPSIEHYGCMVDLLGRAGLLEEAKELIKTMPMKPNAIIWGALLKACRIHRDFLVGGQIGA 441

Query: 361 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEFL 420
           HLVEVDSDHSGRYIQLATILAAEGKWKEAAE+RLKMKNLRV IPPGKS ITL+GVVHEFL
Sbjct: 442 HLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKNLRVPIPPGKSSITLNGVVHEFL 501

Query: 421 AGHQDHPQMKQIHQKLNQVAERLHQ-EGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAF 480
           AGHQDHPQM+QI  KLNQV ERL Q EGY+P TKDL LDLEN+ KET +AQHSEKLAIAF
Sbjct: 502 AGHQDHPQMEQILHKLNQVVERLRQHEGYEPATKDLLLDLENEAKETAVAQHSEKLAIAF 561

Query: 481 GLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           GLINTKPG+TIRVVKNLRVC DCH VAKL+SRIY+R+I+MRDRVRFHHFR G CSC+DYW
Sbjct: 562 GLINTKPGSTIRVVKNLRVCEDCHVVAKLISRIYRREIIMRDRVRFHHFRGGSCSCKDYW 621

BLAST of MS010539 vs. NCBI nr
Match: XP_023543056.1 (pentatricopeptide repeat-containing protein At5g66520 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852.8 bits (2202), Expect = 1.5e-243
Identity = 415/540 (76.85%), Postives = 464/540 (85.93%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WNA+IRAYS+SNEP++AFLLY +M+SSSVP NS+TFPFLLK+CR+ SAM EALQVHGL++
Sbjct: 82  WNAMIRAYSNSNEPELAFLLYRRMLSSSVPHNSYTFPFLLKACRNFSAMSEALQVHGLVI 141

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERD---------------------- 120
           KLGFGSDVFA+N+L+  YALCGDIQYARQLFD+IPERD                      
Sbjct: 142 KLGFGSDVFALNALLHVYALCGDIQYARQLFDNIPERDIVSWNIMIDGYIKSGDVKTAYG 201

Query: 121 --------NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDGVAVASMLTACANLGA 180
                   NVVSWTS+ISGLVEAGL VEAL LC EMQ AGFELDGVA+AS+LTACANLGA
Sbjct: 202 VFLDMPLKNVVSWTSLISGLVEAGLNVEALSLCHEMQNAGFELDGVAIASLLTACANLGA 261

Query: 181 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 240
           L+QGRWLHFYVLNNGVH+DRVI CALVNMY+KCGD+EEAL+ F +LK   KDV+VWTAMI
Sbjct: 262 LDQGRWLHFYVLNNGVHVDRVIGCALVNMYLKCGDMEEALREFGKLKGDQKDVYVWTAMI 321

Query: 241 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 300
           +GFAI+GRGVEALEWF +M REG RPNSITFTA+L ACSY GLVEEGK LF+SM S+YNL
Sbjct: 322 DGFAIHGRGVEALEWFKRMLREGIRPNSITFTAVLRACSYAGLVEEGKVLFESMMSVYNL 381

Query: 301 SPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 360
           SP IEHYGCMVDLLGRAGLL+E KELI+ MPMKPNA+IWGALLKAC+IH DF + GQIG 
Sbjct: 382 SPSIEHYGCMVDLLGRAGLLEEAKELIKTMPMKPNAIIWGALLKACRIHRDFLVGGQIGA 441

Query: 361 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEFL 420
           HLVEVDSDHSGRYIQLATILAAEGKWKEAAE+RLKMKNLR+ IPPGKS ITL+GVVH+FL
Sbjct: 442 HLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKNLRLPIPPGKSSITLNGVVHQFL 501

Query: 421 AGHQDHPQMKQIHQKLNQVAERLHQ-EGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAF 480
           AGHQDHPQM+QI  KLNQV ERL Q EGY+P TKDL LDLEN+ KET +AQHSEKLAIAF
Sbjct: 502 AGHQDHPQMEQILHKLNQVVERLRQHEGYEPATKDLLLDLENEAKETAVAQHSEKLAIAF 561

Query: 481 GLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           GLINTKPG+TIRVVKNLRVC DCH VAKL+SRIY+R+I+MRDRVRFHHFR G CSC DYW
Sbjct: 562 GLINTKPGSTIRVVKNLRVCEDCHVVAKLISRIYRREIIMRDRVRFHHFRGGNCSCNDYW 621

BLAST of MS010539 vs. NCBI nr
Match: XP_022949774.1 (pentatricopeptide repeat-containing protein At5g66520 [Cucurbita moschata])

HSP 1 Score: 850.5 bits (2196), Expect = 7.4e-243
Identity = 414/540 (76.67%), Postives = 464/540 (85.93%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WNA+IRAYS+SNEP++AFLLY QM+SSSVP NS+TFPFLLK+CR+ SAM EALQVHGL++
Sbjct: 82  WNAMIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFLLKACRNFSAMSEALQVHGLVI 141

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERD---------------------- 120
           KLGFGSDVFA+N+L+  YALCGDIQYARQLFD+IPERD                      
Sbjct: 142 KLGFGSDVFALNALLHVYALCGDIQYARQLFDNIPERDIVSWNIMIDGYIKSGDVKTAYG 201

Query: 121 --------NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDGVAVASMLTACANLGA 180
                   NVVSWTS+ISGLVEAGL VEAL LC EMQ AGFELDGVA+AS+LTACANLGA
Sbjct: 202 VFLDMPLKNVVSWTSLISGLVEAGLNVEALSLCHEMQNAGFELDGVAIASLLTACANLGA 261

Query: 181 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 240
           L+QGRWLHFYVLNNGVH+DRVI CALVNMY+KCGD+EEAL+ F +LK   KDV+VWTAMI
Sbjct: 262 LDQGRWLHFYVLNNGVHVDRVIGCALVNMYLKCGDMEEALREFGKLKGDQKDVYVWTAMI 321

Query: 241 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 300
           +GFAI+GRGVEALEWF +M REG RPNSITFTA+L ACSY GLVEEGK LF+SM S+YNL
Sbjct: 322 DGFAIHGRGVEALEWFKRMLREGIRPNSITFTAVLRACSYAGLVEEGKVLFESMMSVYNL 381

Query: 301 SPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 360
           SP IEHYGCMVDLLGRAGLL+E KELI+ MPM+PNA+IWGALLKAC+IH DF + GQIG 
Sbjct: 382 SPSIEHYGCMVDLLGRAGLLEEAKELIKTMPMEPNAIIWGALLKACRIHRDFLVGGQIGA 441

Query: 361 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEFL 420
           HLVEVDSDHSGRYIQLATILAAEGKWKEAAE+RLKMKNLR+ IPPGKS ITL+GVVHEFL
Sbjct: 442 HLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKNLRLPIPPGKSSITLNGVVHEFL 501

Query: 421 AGHQDHPQMKQIHQKLNQVAERLHQ-EGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAF 480
           AGHQDHPQM+QI  KLNQV ERL Q EGY+P TKDL LDLE++ KET +AQHSEKLAIAF
Sbjct: 502 AGHQDHPQMEQILHKLNQVVERLRQHEGYEPATKDLLLDLESEAKETAVAQHSEKLAIAF 561

Query: 481 GLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           GLINTKPG+TIRVVKNLRVC DCH VAKL+S+IY+R+I+MRDRVRFHHFR G CSC DYW
Sbjct: 562 GLINTKPGSTIRVVKNLRVCEDCHVVAKLISQIYRREIIMRDRVRFHHFRGGNCSCNDYW 621

BLAST of MS010539 vs. NCBI nr
Match: KAG6603968.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 847.4 bits (2188), Expect = 6.3e-242
Identity = 413/540 (76.48%), Postives = 463/540 (85.74%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WNA+IRAYS+SNEP++AFLLY QM+SSSVP NS+TFPFLLK+CR+ SAM EALQVHGL++
Sbjct: 82  WNAMIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFLLKACRNFSAMSEALQVHGLVI 141

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERD---------------------- 120
           KLGFGSDVFA+N+L+  YALCGDIQYARQLFD+IPERD                      
Sbjct: 142 KLGFGSDVFALNALLHVYALCGDIQYARQLFDNIPERDIVSWNIMIDGYIKSGDVKTAYG 201

Query: 121 --------NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDGVAVASMLTACANLGA 180
                   NVVSWTS+ISGLVEAGL VEAL LC EMQ AG ELDGVA+AS+LTACANLGA
Sbjct: 202 VFLDMPLKNVVSWTSLISGLVEAGLNVEALSLCHEMQNAGCELDGVAIASLLTACANLGA 261

Query: 181 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 240
           L+QGRWLHFYVLNNGVH+DRVI CALVNMY+KCGD+EEAL+ F +LK   KDV+VWTAMI
Sbjct: 262 LDQGRWLHFYVLNNGVHVDRVIGCALVNMYLKCGDMEEALREFGKLKGDQKDVYVWTAMI 321

Query: 241 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 300
           +GFAI+GRGVEALEWF +M REG RPNSITFTA+L ACSY GLVEEGK LF+SM S+YNL
Sbjct: 322 DGFAIHGRGVEALEWFKRMLREGIRPNSITFTAVLRACSYAGLVEEGKVLFESMMSVYNL 381

Query: 301 SPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 360
           SP IEHYGCMVDLLGRAGLL+E KELI+ MPM+PNA+IWGALLKAC+IH DF + GQIG 
Sbjct: 382 SPSIEHYGCMVDLLGRAGLLEEAKELIKTMPMEPNAIIWGALLKACRIHRDFLVGGQIGA 441

Query: 361 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEFL 420
           HLVEVDSDHSGRYIQLATILAAEGKWKEAAE+RLKMKNLR+ IPPGKS ITL+GVVHEFL
Sbjct: 442 HLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKNLRLPIPPGKSSITLNGVVHEFL 501

Query: 421 AGHQDHPQMKQIHQKLNQVAERLHQ-EGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAF 480
           AGHQDHPQM+QI  KLNQV ERL Q EGY+P TKDL LDLE++ KET +AQHSEKLAIAF
Sbjct: 502 AGHQDHPQMEQILHKLNQVVERLRQHEGYEPATKDLLLDLESEAKETAVAQHSEKLAIAF 561

Query: 481 GLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           GLINTKPG+TIRVVKNLRVC DCH VAKL+S+IY+R+I+MRDRVRFHHFR G CSC DYW
Sbjct: 562 GLINTKPGSTIRVVKNLRVCEDCHVVAKLISQIYRREIIMRDRVRFHHFRGGNCSCNDYW 621

BLAST of MS010539 vs. ExPASy Swiss-Prot
Match: Q9FJY7 (Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H61 PE=2 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 3.2e-164
Identity = 285/540 (52.78%), Postives = 372/540 (68.89%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WN +IR +S S+EP+ + LLY +M+ SS P N++TFP LLK+C +LSA  E  Q+H  I 
Sbjct: 83  WNLMIRGFSCSDEPERSLLLYQRMLCSSAPHNAYTFPSLLKACSNLSAFEETTQIHAQIT 142

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERD---------------------- 120
           KLG+ +DV+A+NSLI +YA+ G+ + A  LFD IPE D                      
Sbjct: 143 KLGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEPDDVSWNSVIKGYVKAGKMDIALT 202

Query: 121 --------NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDGVAVASMLTACANLGA 180
                   N +SWT+MISG V+A +  EAL+L  EMQ +  E D V++A+ L+ACA LGA
Sbjct: 203 LFRKMAEKNAISWTTMISGYVQADMNKEALQLFHEMQNSDVEPDNVSLANALSACAQLGA 262

Query: 181 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 240
           LEQG+W+H Y+    + +D V+ C L++MY KCG++EEAL+VF  +K   K V  WTA+I
Sbjct: 263 LEQGKWIHSYLNKTRIRMDSVLGCVLIDMYAKCGEMEEALEVFKNIKK--KSVQAWTALI 322

Query: 241 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 300
            G+A +G G EA+  F +MQ+ G +PN ITFTA+LTACSY GLVEEGK +F SM+  YNL
Sbjct: 323 SGYAYHGHGREAISKFMEMQKMGIKPNVITFTAVLTACSYTGLVEEGKLIFYSMERDYNL 382

Query: 301 SPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 360
            P IEHYGC+VDLLGRAGLL E K  I+ MP+KPNAVIWGALLKAC+IH +  L  +IG 
Sbjct: 383 KPTIEHYGCIVDLLGRAGLLDEAKRFIQEMPLKPNAVIWGALLKACRIHKNIELGEEIGE 442

Query: 361 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEFL 420
            L+ +D  H GRY+  A I A + KW +AAE R  MK   V   PG S I+L+G  HEFL
Sbjct: 443 ILIAIDPYHGGRYVHKANIHAMDKKWDKAAETRRLMKEQGVAKVPGCSTISLEGTTHEFL 502

Query: 421 AGHQDHPQMKQIHQKLNQVAERLHQEGYKPVTKDLWLDL-ENDEKETVMAQHSEKLAIAF 480
           AG + HP++++I  K   +  +L + GY P  +++ LDL ++DE+E ++ QHSEKLAI +
Sbjct: 503 AGDRSHPEIEKIQSKWRIMRRKLEENGYVPELEEMLLDLVDDDEREAIVHQHSEKLAITY 562

Query: 481 GLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           GLI TKPGT IR++KNLRVC DCH V KL+S+IYKRDIVMRDR RFHHFRDG+CSC DYW
Sbjct: 563 GLIKTKPGTIIRIMKNLRVCKDCHKVTKLISKIYKRDIVMRDRTRFHHFRDGKCSCGDYW 620

BLAST of MS010539 vs. ExPASy Swiss-Prot
Match: Q9FI80 (Pentatricopeptide repeat-containing protein At5g48910 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H38 PE=2 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 1.2e-134
Identity = 242/557 (43.45%), Postives = 349/557 (62.66%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPK--IAFLLYCQMISSS-VPPNSHTFPFLLKSCRDLSAMGEALQVHG 60
           WN IIR +S+S+E K  IA  L+ +M+S   V PN  TFP +LK+C     + E  Q+HG
Sbjct: 93  WNTIIRGFSESDEDKALIAITLFYEMMSDEFVEPNRFTFPSVLKACAKTGKIQEGKQIHG 152

Query: 61  LIVKLGFGSDVFAMNSLIRAYALC------------------------------------ 120
           L +K GFG D F M++L+R Y +C                                    
Sbjct: 153 LALKYGFGGDEFVMSNLVRMYVMCGFMKDARVLFYKNIIEKDMVVMTDRRKRDGEIVLWN 212

Query: 121 ---------GDIQYARQLFDHIPERDNVVSWTSMISGLVEAGLGVEALELCCEMQRAGFE 180
                    GD + AR LFD + +R +VVSW +MISG    G   +A+E+  EM++    
Sbjct: 213 VMIDGYMRLGDCKAARMLFDKMRQR-SVVSWNTMISGYSLNGFFKDAVEVFREMKKGDIR 272

Query: 181 LDGVAVASMLTACANLGALEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQV 240
            + V + S+L A + LG+LE G WLH Y  ++G+ +D V+  AL++MY KCG +E+A+ V
Sbjct: 273 PNYVTLVSVLPAISRLGSLELGEWLHLYAEDSGIRIDDVLGSALIDMYSKCGIIEKAIHV 332

Query: 241 FAELKASLKDVHVWTAMIEGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGG 300
           F  L    ++V  W+AMI GFAI+G+  +A++ F KM++ G RP+ + +  +LTACS+GG
Sbjct: 333 FERLPR--ENVITWSAMINGFAIHGQAGDAIDCFCKMRQAGVRPSDVAYINLLTACSHGG 392

Query: 301 LVEEGKSLFKSMKSLYNLSPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGAL 360
           LVEEG+  F  M S+  L P IEHYGCMVDLLGR+GLL E +E I  MP+KP+ VIW AL
Sbjct: 393 LVEEGRRYFSQMVSVDGLEPRIEHYGCMVDLLGRSGLLDEAEEFILNMPIKPDDVIWKAL 452

Query: 361 LKACQIHGDFFLAGQIGTHLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVL 420
           L AC++ G+  +  ++   L+++    SG Y+ L+ + A++G W E +E+RL+MK   + 
Sbjct: 453 LGACRMQGNVEMGKRVANILMDMVPHDSGAYVALSNMYASQGNWSEVSEMRLRMKEKDIR 512

Query: 421 IPPGKSLITLDGVVHEFLAGHQDHPQMKQIHQKLNQVAERLHQEGYKPVTKDLWLDLEND 480
             PG SLI +DGV+HEF+     HP+ K+I+  L +++++L   GY+P+T  + L+LE +
Sbjct: 513 KDPGCSLIDIDGVLHEFVVEDDSHPKAKEINSMLVEISDKLRLAGYRPITTQVLLNLEEE 572

Query: 481 EKETVMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDR 510
           +KE V+  HSEK+A AFGLI+T PG  IR+VKNLR+C DCH+  KL+S++YKR I +RDR
Sbjct: 573 DKENVLHYHSEKIATAFGLISTSPGKPIRIVKNLRICEDCHSSIKLISKVYKRKITVRDR 632

BLAST of MS010539 vs. ExPASy Swiss-Prot
Match: A8MQA3 (Pentatricopeptide repeat-containing protein At4g21065 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H28 PE=2 SV=2)

HSP 1 Score: 453.4 bits (1165), Expect = 3.5e-126
Identity = 224/511 (43.84%), Postives = 330/511 (64.58%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQM-ISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLI 60
           WN +IR Y++      AF LY +M +S  V P++HT+PFL+K+   ++ +     +H ++
Sbjct: 88  WNTLIRGYAEIGNSISAFSLYREMRVSGLVEPDTHTYPFLIKAVTTMADVRLGETIHSVV 147

Query: 61  VKLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERDNVVSWTSMISGLVEAGLGVEA 120
           ++ GFGS ++  NSL+  YA CGD+  A ++FD +PE+D +V+W S+I+G  E G   EA
Sbjct: 148 IRSGFGSLIYVQNSLLHLYANCGDVASAYKVFDKMPEKD-LVAWNSVINGFAENGKPEEA 207

Query: 121 LELCCEMQRAGFELDGVAVASMLTACANLGALEQGRWLHFYVLNNGVHLDRVIACALVNM 180
           L L  EM   G + DG  + S+L+ACA +GAL  G+ +H Y++  G+  +   +  L+++
Sbjct: 208 LALYTEMNSKGIKPDGFTIVSLLSACAKIGALTLGKRVHVYMIKVGLTRNLHSSNVLLDL 267

Query: 181 YVKCGDLEEALQVFAELKASLKDVHVWTAMIEGFAINGRGVEALEWFNKMQ-REGTRPNS 240
           Y +CG +EEA  +F E+    K+   WT++I G A+NG G EA+E F  M+  EG  P  
Sbjct: 268 YARCGRVEEAKTLFDEMVD--KNSVSWTSLIVGLAVNGFGKEAIELFKYMESTEGLLPCE 327

Query: 241 ITFTAILTACSYGGLVEEGKSLFKSMKSLYNLSPCIEHYGCMVDLLGRAGLLQEVKELIE 300
           ITF  IL ACS+ G+V+EG   F+ M+  Y + P IEH+GCMVDLL RAG +++  E I+
Sbjct: 328 ITFVGILYACSHCGMVKEGFEYFRRMREEYKIEPRIEHFGCMVDLLARAGQVKKAYEYIK 387

Query: 301 MMPMKPNAVIWGALLKACQIHGDFFLAGQIGTHLVEVDSDHSGRYIQLATILAAEGKWKE 360
            MPM+PN VIW  LL AC +HGD  LA      +++++ +HSG Y+ L+ + A+E +W +
Sbjct: 388 SMPMQPNVVIWRTLLGACTVHGDSDLAEFARIQILQLEPNHSGDYVLLSNMYASEQRWSD 447

Query: 361 AAELRLKMKNLRVLIPPGKSLITLDGVVHEFLAGHQDHPQMKQIHQKLNQVAERLHQEGY 420
             ++R +M    V   PG SL+ +   VHEFL G + HPQ   I+ KL ++  RL  EGY
Sbjct: 448 VQKIRKQMLRDGVKKVPGHSLVEVGNRVHEFLMGDKSHPQSDAIYAKLKEMTGRLRSEGY 507

Query: 421 KPVTKDLWLDLENDEKETVMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCGDCHAVAKL 480
            P   ++++D+E +EKE  +  HSEK+AIAF LI+T   + I VVKNLRVC DCH   KL
Sbjct: 508 VPQISNVYVDVEEEEKENAVVYHSEKIAIAFMLISTPERSPITVVKNLRVCADCHLAIKL 567

Query: 481 VSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           VS++Y R+IV+RDR RFHHF++G CSC+DYW
Sbjct: 568 VSKVYNREIVVRDRSRFHHFKNGSCSCQDYW 595

BLAST of MS010539 vs. ExPASy Swiss-Prot
Match: Q9LN01 (Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H12 PE=2 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 2.1e-123
Identity = 215/511 (42.07%), Postives = 333/511 (65.17%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WNA+I  Y+++   K A  L+  M+ ++V P+  T   ++ +C    ++    QVH  I 
Sbjct: 234 WNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSACAQSGSIELGRQVHLWID 293

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERDNVVSWTSMISGLVEAGLGVEAL 120
             GFGS++  +N+LI  Y+ CG+++ A  LF+ +P +D V+SW ++I G     L  EAL
Sbjct: 294 DHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKD-VISWNTLIGGYTHMNLYKEAL 353

Query: 121 ELCCEMQRAGFELDGVAVASMLTACANLGALEQGRWLHFYVLN--NGVHLDRVIACALVN 180
            L  EM R+G   + V + S+L ACA+LGA++ GRW+H Y+     GV     +  +L++
Sbjct: 354 LLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDKRLKGVTNASSLRTSLID 413

Query: 181 MYVKCGDLEEALQVFAELKASLKDVHVWTAMIEGFAINGRGVEALEWFNKMQREGTRPNS 240
           MY KCGD+E A QVF  +    K +  W AMI GFA++GR   + + F++M++ G +P+ 
Sbjct: 414 MYAKCGDIEAAHQVFNSILH--KSLSSWNAMIFGFAMHGRADASFDLFSRMRKIGIQPDD 473

Query: 241 ITFTAILTACSYGGLVEEGKSLFKSMKSLYNLSPCIEHYGCMVDLLGRAGLLQEVKELIE 300
           ITF  +L+ACS+ G+++ G+ +F++M   Y ++P +EHYGCM+DLLG +GL +E +E+I 
Sbjct: 474 ITFVGLLSACSHSGMLDLGRHIFRTMTQDYKMTPKLEHYGCMIDLLGHSGLFKEAEEMIN 533

Query: 301 MMPMKPNAVIWGALLKACQIHGDFFLAGQIGTHLVEVDSDHSGRYIQLATILAAEGKWKE 360
           MM M+P+ VIW +LLKAC++HG+  L      +L++++ ++ G Y+ L+ I A+ G+W E
Sbjct: 534 MMEMEPDGVIWCSLLKACKMHGNVELGESFAENLIKIEPENPGSYVLLSNIYASAGRWNE 593

Query: 361 AAELRLKMKNLRVLIPPGKSLITLDGVVHEFLAGHQDHPQMKQIHQKLNQVAERLHQEGY 420
            A+ R  + +  +   PG S I +D VVHEF+ G + HP+ ++I+  L ++   L + G+
Sbjct: 594 VAKTRALLNDKGMKKVPGCSSIEIDSVVHEFIIGDKFHPRNREIYGMLEEMEVLLEKAGF 653

Query: 421 KPVTKDLWLDLENDEKETVMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCGDCHAVAKL 480
            P T ++  ++E + KE  +  HSEKLAIAFGLI+TKPGT + +VKNLRVC +CH   KL
Sbjct: 654 VPDTSEVLQEMEEEWKEGALRHHSEKLAIAFGLISTKPGTKLTIVKNLRVCRNCHEATKL 713

Query: 481 VSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           +S+IYKR+I+ RDR RFHHFRDG CSC DYW
Sbjct: 714 ISKIYKREIIARDRTRFHHFRDGVCSCNDYW 741

BLAST of MS010539 vs. ExPASy Swiss-Prot
Match: Q9FG16 (Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H88 PE=3 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 8.0e-123
Identity = 227/541 (41.96%), Postives = 342/541 (63.22%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           +N +IR +S   EP  AF  Y QM+ S + P++ TFPFL+K+  ++  +    Q H  IV
Sbjct: 85  FNLLIRCFSTGAEPSKAFGFYTQMLKSRIWPDNITFPFLIKASSEMECVLVGEQTHSQIV 144

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERDNVVSWTSMISGLVEAGLGVEAL 120
           + GF +DV+  NSL+  YA CG I  A ++F  +  RD VVSWTSM++G  + G+   A 
Sbjct: 145 RFGFQNDVYVENSLVHMYANCGFIAAAGRIFGQMGFRD-VVSWTSMVAGYCKCGMVENAR 204

Query: 121 EL-----------------------CCE--------MQRAGFELDGVAVASMLTACANLG 180
           E+                       C E        M+R G   +   + S++++CA+LG
Sbjct: 205 EMFDEMPHRNLFTWSIMINGYAKNNCFEKAIDLFEFMKREGVVANETVMVSVISSCAHLG 264

Query: 181 ALEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAM 240
           ALE G   + YV+ + + ++ ++  ALV+M+ +CGD+E+A+ VF  L  +  D   W+++
Sbjct: 265 ALEFGERAYEYVVKSHMTVNLILGTALVDMFWRCGDIEKAIHVFEGLPET--DSLSWSSI 324

Query: 241 IEGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYN 300
           I+G A++G   +A+ +F++M   G  P  +TFTA+L+ACS+GGLVE+G  ++++MK  + 
Sbjct: 325 IKGLAVHGHAHKAMHYFSQMISLGFIPRDVTFTAVLSACSHGGLVEKGLEIYENMKKDHG 384

Query: 301 LSPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIG 360
           + P +EHYGC+VD+LGRAG L E +  I  M +KPNA I GALL AC+I+ +  +A ++G
Sbjct: 385 IEPRLEHYGCIVDMLGRAGKLAEAENFILKMHVKPNAPILGALLGACKIYKNTEVAERVG 444

Query: 361 THLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEF 420
             L++V  +HSG Y+ L+ I A  G+W +   LR  MK   V  PPG SLI +DG +++F
Sbjct: 445 NMLIKVKPEHSGYYVLLSNIYACAGQWDKIESLRDMMKEKLVKKPPGWSLIEIDGKINKF 504

Query: 421 LAG-HQDHPQMKQIHQKLNQVAERLHQEGYKPVTKDLWLDLENDEKETVMAQHSEKLAIA 480
             G  Q HP+M +I +K  ++  ++   GYK  T D + D++ +EKE+ +  HSEKLAIA
Sbjct: 505 TMGDDQKHPEMGKIRRKWEEILGKIRLIGYKGNTGDAFFDVDEEEKESSIHMHSEKLAIA 564

Query: 481 FGLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDY 510
           +G++ TKPGTTIR+VKNLRVC DCH V KL+S +Y R++++RDR RFHHFR+G CSCRDY
Sbjct: 565 YGMMKTKPGTTIRIVKNLRVCEDCHTVTKLISEVYGRELIVRDRNRFHHFRNGVCSCRDY 622

BLAST of MS010539 vs. ExPASy TrEMBL
Match: A0A6J1BTS5 (pentatricopeptide repeat-containing protein At5g66520 OS=Momordica charantia OX=3673 GN=LOC111005281 PE=3 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 7.9e-291
Identity = 500/539 (92.76%), Postives = 502/539 (93.14%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WNAIIRAYSDSNEPKIAFLLYCQM+SSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV
Sbjct: 78  WNAIIRAYSDSNEPKIAFLLYCQMLSSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 137

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERD---------------------- 120
           KLGFGSDVFAMNSLIRAY LCGDIQYARQLFDHIPERD                      
Sbjct: 138 KLGFGSDVFAMNSLIRAYTLCGDIQYARQLFDHIPERDVVSWNTMVDGYIKFGDVKSAYG 197

Query: 121 --------NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDGVAVASMLTACANLGA 180
                   NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELD VAVASMLTACANLGA
Sbjct: 198 VFLEMPLKNVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDAVAVASMLTACANLGA 257

Query: 181 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 240
           LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI
Sbjct: 258 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 317

Query: 241 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 300
           EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL
Sbjct: 318 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 377

Query: 301 SPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 360
           SPCIEHYGCMVDLLGRAGLLQE KELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT
Sbjct: 378 SPCIEHYGCMVDLLGRAGLLQEAKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 437

Query: 361 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEFL 420
           HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMK LRVLIPPGKSLITLDGVVHEFL
Sbjct: 438 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKKLRVLIPPGKSLITLDGVVHEFL 497

Query: 421 AGHQDHPQMKQIHQKLNQVAERLHQEGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAFG 480
           AGHQDHPQMKQIHQKLNQVAERL +EGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAFG
Sbjct: 498 AGHQDHPQMKQIHQKLNQVAERLRKEGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAFG 557

Query: 481 LINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           LIN KPGTTIRVVKNLRVCGDCH VAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW
Sbjct: 558 LINMKPGTTIRVVKNLRVCGDCHDVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 616

BLAST of MS010539 vs. ExPASy TrEMBL
Match: A0A6J1IT43 (pentatricopeptide repeat-containing protein At5g66520 OS=Cucurbita maxima OX=3661 GN=LOC111478422 PE=3 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.5e-244
Identity = 418/540 (77.41%), Postives = 464/540 (85.93%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WNA+IRAYS+SNEP++AFLLY QM+SSSVP NS+TFPFLLK+CR+ SAM EALQVHGL++
Sbjct: 82  WNAMIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFLLKACRNFSAMSEALQVHGLVI 141

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERD---------------------- 120
           KLGFGSDVFA+N+L+  YALCGDIQYARQLFD+IPERD                      
Sbjct: 142 KLGFGSDVFALNALLHVYALCGDIQYARQLFDNIPERDIVSWNIMIDGYIKSGDVKTAYG 201

Query: 121 --------NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDGVAVASMLTACANLGA 180
                   NVVSWTS+ISGLVEAGL VEAL LC EMQ AGFELDGVA+AS+LTACANLGA
Sbjct: 202 VFLDMPLKNVVSWTSLISGLVEAGLNVEALSLCHEMQNAGFELDGVAIASLLTACANLGA 261

Query: 181 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 240
           L+QGRWLHFYVLNNGVH+DRVI CALVNMY+KCGD+EEALQ F +LK   KDV+VWTAMI
Sbjct: 262 LDQGRWLHFYVLNNGVHVDRVIGCALVNMYLKCGDMEEALQEFGKLKGDQKDVYVWTAMI 321

Query: 241 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 300
           +GFAI+GRGVEALEWF +M REG RPNSITFTA+L ACSY GLVEEGK LF+SM S+Y L
Sbjct: 322 DGFAIHGRGVEALEWFKRMLREGIRPNSITFTAVLRACSYAGLVEEGKVLFESMMSVYIL 381

Query: 301 SPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 360
           SP IEHYGCMVDLLGRAGLL+E KELI+ MPMKPNA+IWGALLKAC+IH DF + GQIG 
Sbjct: 382 SPSIEHYGCMVDLLGRAGLLEEAKELIKTMPMKPNAIIWGALLKACRIHRDFLVGGQIGA 441

Query: 361 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEFL 420
           HLVEVDSDHSGRYIQLATILAAEGKWKEAAE+RLKMKNLRV IPPGKS ITL+GVVHEFL
Sbjct: 442 HLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKNLRVPIPPGKSSITLNGVVHEFL 501

Query: 421 AGHQDHPQMKQIHQKLNQVAERLHQ-EGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAF 480
           AGHQDHPQM+QI  KLNQV ERL Q EGY+P TKDL LDLEN+ KET +AQHSEKLAIAF
Sbjct: 502 AGHQDHPQMEQILHKLNQVVERLRQHEGYEPATKDLLLDLENEAKETAVAQHSEKLAIAF 561

Query: 481 GLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           GLINTKPG+TIRVVKNLRVC DCH VAKL+SRIY+R+I+MRDRVRFHHFR G CSC+DYW
Sbjct: 562 GLINTKPGSTIRVVKNLRVCEDCHVVAKLISRIYRREIIMRDRVRFHHFRGGSCSCKDYW 621

BLAST of MS010539 vs. ExPASy TrEMBL
Match: A0A6J1GDX2 (pentatricopeptide repeat-containing protein At5g66520 OS=Cucurbita moschata OX=3662 GN=LOC111453066 PE=3 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 3.6e-243
Identity = 414/540 (76.67%), Postives = 464/540 (85.93%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WNA+IRAYS+SNEP++AFLLY QM+SSSVP NS+TFPFLLK+CR+ SAM EALQVHGL++
Sbjct: 82  WNAMIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFLLKACRNFSAMSEALQVHGLVI 141

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERD---------------------- 120
           KLGFGSDVFA+N+L+  YALCGDIQYARQLFD+IPERD                      
Sbjct: 142 KLGFGSDVFALNALLHVYALCGDIQYARQLFDNIPERDIVSWNIMIDGYIKSGDVKTAYG 201

Query: 121 --------NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDGVAVASMLTACANLGA 180
                   NVVSWTS+ISGLVEAGL VEAL LC EMQ AGFELDGVA+AS+LTACANLGA
Sbjct: 202 VFLDMPLKNVVSWTSLISGLVEAGLNVEALSLCHEMQNAGFELDGVAIASLLTACANLGA 261

Query: 181 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 240
           L+QGRWLHFYVLNNGVH+DRVI CALVNMY+KCGD+EEAL+ F +LK   KDV+VWTAMI
Sbjct: 262 LDQGRWLHFYVLNNGVHVDRVIGCALVNMYLKCGDMEEALREFGKLKGDQKDVYVWTAMI 321

Query: 241 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 300
           +GFAI+GRGVEALEWF +M REG RPNSITFTA+L ACSY GLVEEGK LF+SM S+YNL
Sbjct: 322 DGFAIHGRGVEALEWFKRMLREGIRPNSITFTAVLRACSYAGLVEEGKVLFESMMSVYNL 381

Query: 301 SPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 360
           SP IEHYGCMVDLLGRAGLL+E KELI+ MPM+PNA+IWGALLKAC+IH DF + GQIG 
Sbjct: 382 SPSIEHYGCMVDLLGRAGLLEEAKELIKTMPMEPNAIIWGALLKACRIHRDFLVGGQIGA 441

Query: 361 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEFL 420
           HLVEVDSDHSGRYIQLATILAAEGKWKEAAE+RLKMKNLR+ IPPGKS ITL+GVVHEFL
Sbjct: 442 HLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKNLRLPIPPGKSSITLNGVVHEFL 501

Query: 421 AGHQDHPQMKQIHQKLNQVAERLHQ-EGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAF 480
           AGHQDHPQM+QI  KLNQV ERL Q EGY+P TKDL LDLE++ KET +AQHSEKLAIAF
Sbjct: 502 AGHQDHPQMEQILHKLNQVVERLRQHEGYEPATKDLLLDLESEAKETAVAQHSEKLAIAF 561

Query: 481 GLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           GLINTKPG+TIRVVKNLRVC DCH VAKL+S+IY+R+I+MRDRVRFHHFR G CSC DYW
Sbjct: 562 GLINTKPGSTIRVVKNLRVCEDCHVVAKLISQIYRREIIMRDRVRFHHFRGGNCSCNDYW 621

BLAST of MS010539 vs. ExPASy TrEMBL
Match: A0A5D3CKZ8 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002030 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.7e-240
Identity = 411/540 (76.11%), Postives = 460/540 (85.19%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WNAIIRAYS+S EP++AFLLY QM+SSSVP NS+TFPFLLK+CR+LSA+GEALQVHGL++
Sbjct: 78  WNAIIRAYSNSKEPELAFLLYQQMLSSSVPHNSYTFPFLLKACRNLSALGEALQVHGLVI 137

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERD---------------------- 120
           KLGFGSDVFA+N+L+  YALCG+I+YARQ+FD+IPERD                      
Sbjct: 138 KLGFGSDVFALNALLHVYALCGEIRYARQMFDNIPERDAVSWNIMIDGYIKSGDVKTAYG 197

Query: 121 --------NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDGVAVASMLTACANLGA 180
                   NVVSWTS+ISGLV AGL V+AL LC EMQ AGFELDGVA+A +LTACANLGA
Sbjct: 198 IFLDMPSKNVVSWTSLISGLVGAGLSVKALSLCYEMQNAGFELDGVAIACLLTACANLGA 257

Query: 181 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 240
           L+QGRWLHFYVLNNGV +DRVI CALVNMYVKCGD+EEAL+VF +LK   KDV +WTAMI
Sbjct: 258 LDQGRWLHFYVLNNGVDVDRVIGCALVNMYVKCGDMEEALRVFGKLKGDQKDVCIWTAMI 317

Query: 241 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 300
           +GFAI+GRGVEALEWF+ M+REG RPNSITFTA+L ACSYGGLVEEGK LFKSMK LYNL
Sbjct: 318 DGFAIHGRGVEALEWFDLMRREGIRPNSITFTAVLRACSYGGLVEEGKELFKSMKCLYNL 377

Query: 301 SPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 360
           SP IEHYGCMVDLLGR+G L E KELI+ MPMKPNAVIWGA LKAC IH DF +  QIG 
Sbjct: 378 SPSIEHYGCMVDLLGRSGRLNEAKELIKNMPMKPNAVIWGAFLKACWIHRDFLVGSQIGA 437

Query: 361 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEFL 420
           HLVEVDSDHSGRYIQLATILAA+GKWKEAAE+RLKMKNL V I PGKS ITL+G+VHEFL
Sbjct: 438 HLVEVDSDHSGRYIQLATILAAQGKWKEAAEVRLKMKNLGVPISPGKSSITLNGIVHEFL 497

Query: 421 AGHQDHPQMKQIHQKLNQVAERLHQ-EGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAF 480
           AGHQDHPQM+QIH KL Q+AERL Q EGY+P TKDL LDLEN+EKET +AQHSEKLAIAF
Sbjct: 498 AGHQDHPQMEQIHLKLKQIAERLRQDEGYEPATKDLLLDLENEEKETAIAQHSEKLAIAF 557

Query: 481 GLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           GLINTKPGTTIRVVKNLR+C DCH VAKLVS+IY R+I+MRDRVRFHHFRDG CSC+DYW
Sbjct: 558 GLINTKPGTTIRVVKNLRICRDCHTVAKLVSQIYCREIIMRDRVRFHHFRDGSCSCKDYW 617

BLAST of MS010539 vs. ExPASy TrEMBL
Match: A0A1S3B1S8 (pentatricopeptide repeat-containing protein At5g66520 OS=Cucumis melo OX=3656 GN=LOC103485057 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.7e-240
Identity = 411/540 (76.11%), Postives = 460/540 (85.19%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WNAIIRAYS+S EP++AFLLY QM+SSSVP NS+TFPFLLK+CR+LSA+GEALQVHGL++
Sbjct: 78  WNAIIRAYSNSKEPELAFLLYQQMLSSSVPHNSYTFPFLLKACRNLSALGEALQVHGLVI 137

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERD---------------------- 120
           KLGFGSDVFA+N+L+  YALCG+I+YARQ+FD+IPERD                      
Sbjct: 138 KLGFGSDVFALNALLHVYALCGEIRYARQMFDNIPERDAVSWNIMIDGYIKSGDVKTAYG 197

Query: 121 --------NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDGVAVASMLTACANLGA 180
                   NVVSWTS+ISGLV AGL V+AL LC EMQ AGFELDGVA+A +LTACANLGA
Sbjct: 198 IFLDMPSKNVVSWTSLISGLVGAGLSVKALSLCYEMQNAGFELDGVAIACLLTACANLGA 257

Query: 181 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 240
           L+QGRWLHFYVLNNGV +DRVI CALVNMYVKCGD+EEAL+VF +LK   KDV +WTAMI
Sbjct: 258 LDQGRWLHFYVLNNGVDVDRVIGCALVNMYVKCGDMEEALRVFGKLKGDQKDVCIWTAMI 317

Query: 241 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 300
           +GFAI+GRGVEALEWF+ M+REG RPNSITFTA+L ACSYGGLVEEGK LFKSMK LYNL
Sbjct: 318 DGFAIHGRGVEALEWFDLMRREGIRPNSITFTAVLRACSYGGLVEEGKELFKSMKCLYNL 377

Query: 301 SPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 360
           SP IEHYGCMVDLLGR+G L E KELI+ MPMKPNAVIWGA LKAC IH DF +  QIG 
Sbjct: 378 SPSIEHYGCMVDLLGRSGRLNEAKELIKNMPMKPNAVIWGAFLKACWIHRDFLVGSQIGA 437

Query: 361 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEFL 420
           HLVEVDSDHSGRYIQLATILAA+GKWKEAAE+RLKMKNL V I PGKS ITL+G+VHEFL
Sbjct: 438 HLVEVDSDHSGRYIQLATILAAQGKWKEAAEVRLKMKNLGVPISPGKSSITLNGIVHEFL 497

Query: 421 AGHQDHPQMKQIHQKLNQVAERLHQ-EGYKPVTKDLWLDLENDEKETVMAQHSEKLAIAF 480
           AGHQDHPQM+QIH KL Q+AERL Q EGY+P TKDL LDLEN+EKET +AQHSEKLAIAF
Sbjct: 498 AGHQDHPQMEQIHLKLKQIAERLRQDEGYEPATKDLLLDLENEEKETAIAQHSEKLAIAF 557

Query: 481 GLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           GLINTKPGTTIRVVKNLR+C DCH VAKLVS+IY R+I+MRDRVRFHHFRDG CSC+DYW
Sbjct: 558 GLINTKPGTTIRVVKNLRICRDCHTVAKLVSQIYCREIIMRDRVRFHHFRDGSCSCKDYW 617

BLAST of MS010539 vs. TAIR 10
Match: AT5G66520.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 579.7 bits (1493), Expect = 2.3e-165
Identity = 285/540 (52.78%), Postives = 372/540 (68.89%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WN +IR +S S+EP+ + LLY +M+ SS P N++TFP LLK+C +LSA  E  Q+H  I 
Sbjct: 83  WNLMIRGFSCSDEPERSLLLYQRMLCSSAPHNAYTFPSLLKACSNLSAFEETTQIHAQIT 142

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERD---------------------- 120
           KLG+ +DV+A+NSLI +YA+ G+ + A  LFD IPE D                      
Sbjct: 143 KLGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEPDDVSWNSVIKGYVKAGKMDIALT 202

Query: 121 --------NVVSWTSMISGLVEAGLGVEALELCCEMQRAGFELDGVAVASMLTACANLGA 180
                   N +SWT+MISG V+A +  EAL+L  EMQ +  E D V++A+ L+ACA LGA
Sbjct: 203 LFRKMAEKNAISWTTMISGYVQADMNKEALQLFHEMQNSDVEPDNVSLANALSACAQLGA 262

Query: 181 LEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAMI 240
           LEQG+W+H Y+    + +D V+ C L++MY KCG++EEAL+VF  +K   K V  WTA+I
Sbjct: 263 LEQGKWIHSYLNKTRIRMDSVLGCVLIDMYAKCGEMEEALEVFKNIKK--KSVQAWTALI 322

Query: 241 EGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYNL 300
            G+A +G G EA+  F +MQ+ G +PN ITFTA+LTACSY GLVEEGK +F SM+  YNL
Sbjct: 323 SGYAYHGHGREAISKFMEMQKMGIKPNVITFTAVLTACSYTGLVEEGKLIFYSMERDYNL 382

Query: 301 SPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIGT 360
            P IEHYGC+VDLLGRAGLL E K  I+ MP+KPNAVIWGALLKAC+IH +  L  +IG 
Sbjct: 383 KPTIEHYGCIVDLLGRAGLLDEAKRFIQEMPLKPNAVIWGALLKACRIHKNIELGEEIGE 442

Query: 361 HLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEFL 420
            L+ +D  H GRY+  A I A + KW +AAE R  MK   V   PG S I+L+G  HEFL
Sbjct: 443 ILIAIDPYHGGRYVHKANIHAMDKKWDKAAETRRLMKEQGVAKVPGCSTISLEGTTHEFL 502

Query: 421 AGHQDHPQMKQIHQKLNQVAERLHQEGYKPVTKDLWLDL-ENDEKETVMAQHSEKLAIAF 480
           AG + HP++++I  K   +  +L + GY P  +++ LDL ++DE+E ++ QHSEKLAI +
Sbjct: 503 AGDRSHPEIEKIQSKWRIMRRKLEENGYVPELEEMLLDLVDDDEREAIVHQHSEKLAITY 562

Query: 481 GLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           GLI TKPGT IR++KNLRVC DCH V KL+S+IYKRDIVMRDR RFHHFRDG+CSC DYW
Sbjct: 563 GLIKTKPGTIIRIMKNLRVCKDCHKVTKLISKIYKRDIVMRDRTRFHHFRDGKCSCGDYW 620

BLAST of MS010539 vs. TAIR 10
Match: AT5G48910.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 481.5 bits (1238), Expect = 8.4e-136
Identity = 242/557 (43.45%), Postives = 349/557 (62.66%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPK--IAFLLYCQMISSS-VPPNSHTFPFLLKSCRDLSAMGEALQVHG 60
           WN IIR +S+S+E K  IA  L+ +M+S   V PN  TFP +LK+C     + E  Q+HG
Sbjct: 93  WNTIIRGFSESDEDKALIAITLFYEMMSDEFVEPNRFTFPSVLKACAKTGKIQEGKQIHG 152

Query: 61  LIVKLGFGSDVFAMNSLIRAYALC------------------------------------ 120
           L +K GFG D F M++L+R Y +C                                    
Sbjct: 153 LALKYGFGGDEFVMSNLVRMYVMCGFMKDARVLFYKNIIEKDMVVMTDRRKRDGEIVLWN 212

Query: 121 ---------GDIQYARQLFDHIPERDNVVSWTSMISGLVEAGLGVEALELCCEMQRAGFE 180
                    GD + AR LFD + +R +VVSW +MISG    G   +A+E+  EM++    
Sbjct: 213 VMIDGYMRLGDCKAARMLFDKMRQR-SVVSWNTMISGYSLNGFFKDAVEVFREMKKGDIR 272

Query: 181 LDGVAVASMLTACANLGALEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQV 240
            + V + S+L A + LG+LE G WLH Y  ++G+ +D V+  AL++MY KCG +E+A+ V
Sbjct: 273 PNYVTLVSVLPAISRLGSLELGEWLHLYAEDSGIRIDDVLGSALIDMYSKCGIIEKAIHV 332

Query: 241 FAELKASLKDVHVWTAMIEGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGG 300
           F  L    ++V  W+AMI GFAI+G+  +A++ F KM++ G RP+ + +  +LTACS+GG
Sbjct: 333 FERLPR--ENVITWSAMINGFAIHGQAGDAIDCFCKMRQAGVRPSDVAYINLLTACSHGG 392

Query: 301 LVEEGKSLFKSMKSLYNLSPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGAL 360
           LVEEG+  F  M S+  L P IEHYGCMVDLLGR+GLL E +E I  MP+KP+ VIW AL
Sbjct: 393 LVEEGRRYFSQMVSVDGLEPRIEHYGCMVDLLGRSGLLDEAEEFILNMPIKPDDVIWKAL 452

Query: 361 LKACQIHGDFFLAGQIGTHLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVL 420
           L AC++ G+  +  ++   L+++    SG Y+ L+ + A++G W E +E+RL+MK   + 
Sbjct: 453 LGACRMQGNVEMGKRVANILMDMVPHDSGAYVALSNMYASQGNWSEVSEMRLRMKEKDIR 512

Query: 421 IPPGKSLITLDGVVHEFLAGHQDHPQMKQIHQKLNQVAERLHQEGYKPVTKDLWLDLEND 480
             PG SLI +DGV+HEF+     HP+ K+I+  L +++++L   GY+P+T  + L+LE +
Sbjct: 513 KDPGCSLIDIDGVLHEFVVEDDSHPKAKEINSMLVEISDKLRLAGYRPITTQVLLNLEEE 572

Query: 481 EKETVMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDR 510
           +KE V+  HSEK+A AFGLI+T PG  IR+VKNLR+C DCH+  KL+S++YKR I +RDR
Sbjct: 573 DKENVLHYHSEKIATAFGLISTSPGKPIRIVKNLRICEDCHSSIKLISKVYKRKITVRDR 632

BLAST of MS010539 vs. TAIR 10
Match: AT4G21065.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 453.4 bits (1165), Expect = 2.5e-127
Identity = 224/511 (43.84%), Postives = 330/511 (64.58%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQM-ISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLI 60
           WN +IR Y++      AF LY +M +S  V P++HT+PFL+K+   ++ +     +H ++
Sbjct: 88  WNTLIRGYAEIGNSISAFSLYREMRVSGLVEPDTHTYPFLIKAVTTMADVRLGETIHSVV 147

Query: 61  VKLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERDNVVSWTSMISGLVEAGLGVEA 120
           ++ GFGS ++  NSL+  YA CGD+  A ++FD +PE+D +V+W S+I+G  E G   EA
Sbjct: 148 IRSGFGSLIYVQNSLLHLYANCGDVASAYKVFDKMPEKD-LVAWNSVINGFAENGKPEEA 207

Query: 121 LELCCEMQRAGFELDGVAVASMLTACANLGALEQGRWLHFYVLNNGVHLDRVIACALVNM 180
           L L  EM   G + DG  + S+L+ACA +GAL  G+ +H Y++  G+  +   +  L+++
Sbjct: 208 LALYTEMNSKGIKPDGFTIVSLLSACAKIGALTLGKRVHVYMIKVGLTRNLHSSNVLLDL 267

Query: 181 YVKCGDLEEALQVFAELKASLKDVHVWTAMIEGFAINGRGVEALEWFNKMQ-REGTRPNS 240
           Y +CG +EEA  +F E+    K+   WT++I G A+NG G EA+E F  M+  EG  P  
Sbjct: 268 YARCGRVEEAKTLFDEMVD--KNSVSWTSLIVGLAVNGFGKEAIELFKYMESTEGLLPCE 327

Query: 241 ITFTAILTACSYGGLVEEGKSLFKSMKSLYNLSPCIEHYGCMVDLLGRAGLLQEVKELIE 300
           ITF  IL ACS+ G+V+EG   F+ M+  Y + P IEH+GCMVDLL RAG +++  E I+
Sbjct: 328 ITFVGILYACSHCGMVKEGFEYFRRMREEYKIEPRIEHFGCMVDLLARAGQVKKAYEYIK 387

Query: 301 MMPMKPNAVIWGALLKACQIHGDFFLAGQIGTHLVEVDSDHSGRYIQLATILAAEGKWKE 360
            MPM+PN VIW  LL AC +HGD  LA      +++++ +HSG Y+ L+ + A+E +W +
Sbjct: 388 SMPMQPNVVIWRTLLGACTVHGDSDLAEFARIQILQLEPNHSGDYVLLSNMYASEQRWSD 447

Query: 361 AAELRLKMKNLRVLIPPGKSLITLDGVVHEFLAGHQDHPQMKQIHQKLNQVAERLHQEGY 420
             ++R +M    V   PG SL+ +   VHEFL G + HPQ   I+ KL ++  RL  EGY
Sbjct: 448 VQKIRKQMLRDGVKKVPGHSLVEVGNRVHEFLMGDKSHPQSDAIYAKLKEMTGRLRSEGY 507

Query: 421 KPVTKDLWLDLENDEKETVMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCGDCHAVAKL 480
            P   ++++D+E +EKE  +  HSEK+AIAF LI+T   + I VVKNLRVC DCH   KL
Sbjct: 508 VPQISNVYVDVEEEEKENAVVYHSEKIAIAFMLISTPERSPITVVKNLRVCADCHLAIKL 567

Query: 481 VSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           VS++Y R+IV+RDR RFHHF++G CSC+DYW
Sbjct: 568 VSKVYNREIVVRDRSRFHHFKNGSCSCQDYW 595

BLAST of MS010539 vs. TAIR 10
Match: AT1G08070.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 444.1 bits (1141), Expect = 1.5e-124
Identity = 215/511 (42.07%), Postives = 333/511 (65.17%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           WNA+I  Y+++   K A  L+  M+ ++V P+  T   ++ +C    ++    QVH  I 
Sbjct: 234 WNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSACAQSGSIELGRQVHLWID 293

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERDNVVSWTSMISGLVEAGLGVEAL 120
             GFGS++  +N+LI  Y+ CG+++ A  LF+ +P +D V+SW ++I G     L  EAL
Sbjct: 294 DHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKD-VISWNTLIGGYTHMNLYKEAL 353

Query: 121 ELCCEMQRAGFELDGVAVASMLTACANLGALEQGRWLHFYVLN--NGVHLDRVIACALVN 180
            L  EM R+G   + V + S+L ACA+LGA++ GRW+H Y+     GV     +  +L++
Sbjct: 354 LLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDKRLKGVTNASSLRTSLID 413

Query: 181 MYVKCGDLEEALQVFAELKASLKDVHVWTAMIEGFAINGRGVEALEWFNKMQREGTRPNS 240
           MY KCGD+E A QVF  +    K +  W AMI GFA++GR   + + F++M++ G +P+ 
Sbjct: 414 MYAKCGDIEAAHQVFNSILH--KSLSSWNAMIFGFAMHGRADASFDLFSRMRKIGIQPDD 473

Query: 241 ITFTAILTACSYGGLVEEGKSLFKSMKSLYNLSPCIEHYGCMVDLLGRAGLLQEVKELIE 300
           ITF  +L+ACS+ G+++ G+ +F++M   Y ++P +EHYGCM+DLLG +GL +E +E+I 
Sbjct: 474 ITFVGLLSACSHSGMLDLGRHIFRTMTQDYKMTPKLEHYGCMIDLLGHSGLFKEAEEMIN 533

Query: 301 MMPMKPNAVIWGALLKACQIHGDFFLAGQIGTHLVEVDSDHSGRYIQLATILAAEGKWKE 360
           MM M+P+ VIW +LLKAC++HG+  L      +L++++ ++ G Y+ L+ I A+ G+W E
Sbjct: 534 MMEMEPDGVIWCSLLKACKMHGNVELGESFAENLIKIEPENPGSYVLLSNIYASAGRWNE 593

Query: 361 AAELRLKMKNLRVLIPPGKSLITLDGVVHEFLAGHQDHPQMKQIHQKLNQVAERLHQEGY 420
            A+ R  + +  +   PG S I +D VVHEF+ G + HP+ ++I+  L ++   L + G+
Sbjct: 594 VAKTRALLNDKGMKKVPGCSSIEIDSVVHEFIIGDKFHPRNREIYGMLEEMEVLLEKAGF 653

Query: 421 KPVTKDLWLDLENDEKETVMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCGDCHAVAKL 480
            P T ++  ++E + KE  +  HSEKLAIAFGLI+TKPGT + +VKNLRVC +CH   KL
Sbjct: 654 VPDTSEVLQEMEEEWKEGALRHHSEKLAIAFGLISTKPGTKLTIVKNLRVCRNCHEATKL 713

Query: 481 VSRIYKRDIVMRDRVRFHHFRDGRCSCRDYW 510
           +S+IYKR+I+ RDR RFHHFRDG CSC DYW
Sbjct: 714 ISKIYKREIIARDRTRFHHFRDGVCSCNDYW 741

BLAST of MS010539 vs. TAIR 10
Match: AT5G06540.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 442.2 bits (1136), Expect = 5.7e-124
Identity = 227/541 (41.96%), Postives = 342/541 (63.22%), Query Frame = 0

Query: 1   WNAIIRAYSDSNEPKIAFLLYCQMISSSVPPNSHTFPFLLKSCRDLSAMGEALQVHGLIV 60
           +N +IR +S   EP  AF  Y QM+ S + P++ TFPFL+K+  ++  +    Q H  IV
Sbjct: 85  FNLLIRCFSTGAEPSKAFGFYTQMLKSRIWPDNITFPFLIKASSEMECVLVGEQTHSQIV 144

Query: 61  KLGFGSDVFAMNSLIRAYALCGDIQYARQLFDHIPERDNVVSWTSMISGLVEAGLGVEAL 120
           + GF +DV+  NSL+  YA CG I  A ++F  +  RD VVSWTSM++G  + G+   A 
Sbjct: 145 RFGFQNDVYVENSLVHMYANCGFIAAAGRIFGQMGFRD-VVSWTSMVAGYCKCGMVENAR 204

Query: 121 EL-----------------------CCE--------MQRAGFELDGVAVASMLTACANLG 180
           E+                       C E        M+R G   +   + S++++CA+LG
Sbjct: 205 EMFDEMPHRNLFTWSIMINGYAKNNCFEKAIDLFEFMKREGVVANETVMVSVISSCAHLG 264

Query: 181 ALEQGRWLHFYVLNNGVHLDRVIACALVNMYVKCGDLEEALQVFAELKASLKDVHVWTAM 240
           ALE G   + YV+ + + ++ ++  ALV+M+ +CGD+E+A+ VF  L  +  D   W+++
Sbjct: 265 ALEFGERAYEYVVKSHMTVNLILGTALVDMFWRCGDIEKAIHVFEGLPET--DSLSWSSI 324

Query: 241 IEGFAINGRGVEALEWFNKMQREGTRPNSITFTAILTACSYGGLVEEGKSLFKSMKSLYN 300
           I+G A++G   +A+ +F++M   G  P  +TFTA+L+ACS+GGLVE+G  ++++MK  + 
Sbjct: 325 IKGLAVHGHAHKAMHYFSQMISLGFIPRDVTFTAVLSACSHGGLVEKGLEIYENMKKDHG 384

Query: 301 LSPCIEHYGCMVDLLGRAGLLQEVKELIEMMPMKPNAVIWGALLKACQIHGDFFLAGQIG 360
           + P +EHYGC+VD+LGRAG L E +  I  M +KPNA I GALL AC+I+ +  +A ++G
Sbjct: 385 IEPRLEHYGCIVDMLGRAGKLAEAENFILKMHVKPNAPILGALLGACKIYKNTEVAERVG 444

Query: 361 THLVEVDSDHSGRYIQLATILAAEGKWKEAAELRLKMKNLRVLIPPGKSLITLDGVVHEF 420
             L++V  +HSG Y+ L+ I A  G+W +   LR  MK   V  PPG SLI +DG +++F
Sbjct: 445 NMLIKVKPEHSGYYVLLSNIYACAGQWDKIESLRDMMKEKLVKKPPGWSLIEIDGKINKF 504

Query: 421 LAG-HQDHPQMKQIHQKLNQVAERLHQEGYKPVTKDLWLDLENDEKETVMAQHSEKLAIA 480
             G  Q HP+M +I +K  ++  ++   GYK  T D + D++ +EKE+ +  HSEKLAIA
Sbjct: 505 TMGDDQKHPEMGKIRRKWEEILGKIRLIGYKGNTGDAFFDVDEEEKESSIHMHSEKLAIA 564

Query: 481 FGLINTKPGTTIRVVKNLRVCGDCHAVAKLVSRIYKRDIVMRDRVRFHHFRDGRCSCRDY 510
           +G++ TKPGTTIR+VKNLRVC DCH V KL+S +Y R++++RDR RFHHFR+G CSCRDY
Sbjct: 565 YGMMKTKPGTTIRIVKNLRVCEDCHTVTKLISEVYGRELIVRDRNRFHHFRNGVCSCRDY 622