MS010418 (gene) Bitter gourd (TR) v1

Overview


Name	MS010418
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Cyanate hydratase
Location	scaffold878: 760326 .. 761982 (-)
RNA-Seq Expression	MS010418
Synteny	MS010418

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGTAGTAGCAGTCGCAGAGCAAGCTTAATTCGTGAGCTGCAGGATGCGAAGCAGTCGTCGGGAAAATCTTACAATCAGATTGCCGAAGAGACGGGTCTCACCAACGTCTACGTCGCTCAGCTCCTCAGACGCCAAGCGCACCTTAAGGCCGACACCGTCCCTAAGTTGAGAGCCGCCTTGCCTCACCTCACCCATGAACATATTCAAGAGATGATGCGGCCGCCAATGAGGTGCTACGACCCTTGTTTGATCCAAGAACCTACAATTTACAGGTGGGTCATCACTTAATTGTTTTTTTCTTAAGCTTTATCCATTCTTCTTATCAAGTTCGTTGTGGATTTTTAATCGCTTTTGGTTTGGGGGAAATCTGGTAGTTGAAGCATGCGGTTAATTGTTTCCTTGGTTACTTTAGCTCAGTTCATAACGAGTAAGGATAGTTTCAAGAACGTCGACAGACTGACTGATTGAAATTTTAATTCCTCGATTTGTTGTATTGCTATATGAATAGCTACTGCAAATGCTTGTGGCAAAATTGGTCGGTTTATTGAGCTTAATTTTAGTGGACTTGCTCGTTTGTTGTACTGAATCGGGACTTCCATGGATGTAGATTGAATGAAGCAGTTATGCATTTTGGCGAGAGCATCAAGGAAATTATTAACGAGGATTTTGGCGACGGAATGTAAGTCGGTTTGGCTTAATGTTTTCTTCTTTGCAACGTGAAAGCCATTACGGTTCTTTAACATTTGCAAGTATGTCTTAAAATCAAAATGAATTCAATTCCTCGTTTGATAACTTCTTTGTTTTAACAAGGTTGTTTATAATAATTTCTGTTTTAAGTTCCAACTATCTAAAACCACTTTTATAGAATTTAGATCGAATTTCAACAAAAATAAAACATGCTTTGAGTTTTAGCTTTCAACATGCTAGGAGTTCAAAAACCAATTTCATGGAACCTTTTTAGTTTTCAATAATTCTTCATTAGGAAAACTATTTATACTACAAGGATTTCAGACCCTTCAACTCATACAGCAATGTAAACATTTCTATAAAAGTAGGTTGTATCAGTTTTTGCCCTGGTGGTTGTGCAGCAAACACATTGAGAAATGTAATGGAGCAACGAAGGTTAGGATAATGTTGCAGGTTATTTTTCTTTAATGATGTGGAAGTACAGGAGTTTCCTTGATATTGGTCAGAAACCCGGAAGTTACTGAAAGATGATATTATAGCTGTGGAGCTCATATTAAACATTGTGCCATTTATCATATGAAACTTGTGTCTCAACTTCTCACATAAGCAATTACAACTACAAGTCAAGTTTTCCTTTAATTCCTTGTCCATATCCAATGCGTCAAATAAGAATCTTTAATCATTCATATTATGTTCTTAAGCCCAAACGCTTTTACAACCCAGTATCCTTCAGTTAAGATGACACCTTGCAGTAAAAACCAACCAACTCTGTTATTTTTCCAGTGGTTGAGACCCTGTTGATTAGAGAAGCTAATGTGATTATGTGAAGGTCTAATTTTTATTTGTTAGTATGTCAGCTATAGACTTCTACTGCTCAGTCGACAAGGTCAAGGGTGTTGATGGGAAGGACCGTGTCGTGATAACATTTGATGGTAAATATTTGCCATACACCGAGCAGGTTAGT

mRNA sequence

ATGGAAGGTAGTAGCAGTCGCAGAGCAAGCTTAATTCGTGAGCTGCAGGATGCGAAGCAGTCGTCGGGAAAATCTTACAATCAGATTGCCGAAGAGACGGGTCTCACCAACGTCTACGTCGCTCAGCTCCTCAGACGCCAAGCGCACCTTAAGGCCGACACCGTCCCTAAGTTGAGAGCCGCCTTGCCTCACCTCACCCATGAACATATTCAAGAGATGATGCGGCCGCCAATGAGGTGCTACGACCCTTGTTTGATCCAAGAACCTACAATTTACAGATTGAATGAAGCAGTTATGCATTTTGGCGAGAGCATCAAGGAAATTATTAACGAGGATTTTGGCGACGGAATTATGTCAGCTATAGACTTCTACTGCTCAGTCGACAAGGTCAAGGGTGTTGATGGGAAGGACCGTGTCGTGATAACATTTGATGGTAAATATTTGCCATACACCGAGCAGGTTAGT

Coding sequence (CDS)

ATGGAAGGTAGTAGCAGTCGCAGAGCAAGCTTAATTCGTGAGCTGCAGGATGCGAAGCAGTCGTCGGGAAAATCTTACAATCAGATTGCCGAAGAGACGGGTCTCACCAACGTCTACGTCGCTCAGCTCCTCAGACGCCAAGCGCACCTTAAGGCCGACACCGTCCCTAAGTTGAGAGCCGCCTTGCCTCACCTCACCCATGAACATATTCAAGAGATGATGCGGCCGCCAATGAGGTGCTACGACCCTTGTTTGATCCAAGAACCTACAATTTACAGATTGAATGAAGCAGTTATGCATTTTGGCGAGAGCATCAAGGAAATTATTAACGAGGATTTTGGCGACGGAATTATGTCAGCTATAGACTTCTACTGCTCAGTCGACAAGGTCAAGGGTGTTGATGGGAAGGACCGTGTCGTGATAACATTTGATGGTAAATATTTGCCATACACCGAGCAGGTTAGT

Protein sequence

MEGSSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRAALPHLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSAIDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQVS

Homology

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BLAST of MS010418 vs. NCBI nr
Match: XP_022153411.1 (cyanate hydratase [Momordica charantia])

HSP 1 Score: 307.8 bits (787), Expect = 5.5e-80
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 1   MEGSSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRA 60
           MEGSSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRA
Sbjct: 1   MEGSSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRA 60

Query: 61  ALPHLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSA 120
           ALPHLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSA
Sbjct: 61  ALPHLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSA 120

Query: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 154
           IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ
Sbjct: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 153

[Close]

BLAST of MS010418 vs. NCBI nr
Match: XP_022979374.1 (cyanate hydratase [Cucurbita maxima])

HSP 1 Score: 274.2 bits (700), Expect = 6.7e-70
Identity = 139/153 (90.85%), Postives = 144/153 (94.12%), Query Frame = 0


Query: 1   MEGSSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRA 60
           MEGSS  RAS IR+LQDAK+ SGKS NQIAEETGLTNVYVAQLLRRQA LK +TVPKLRA
Sbjct: 1   MEGSS--RASAIRQLQDAKRLSGKSCNQIAEETGLTNVYVAQLLRRQAQLKPETVPKLRA 60

Query: 61  ALPHLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSA 120
           ALPHLT +HIQEMM PPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINE+FGDGIMSA
Sbjct: 61  ALPHLTDDHIQEMMLPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEEFGDGIMSA 120

Query: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 154
           IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ
Sbjct: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 151

[Close]

BLAST of MS010418 vs. NCBI nr
Match: XP_038879847.1 (cyanate hydratase [Benincasa hispida])

HSP 1 Score: 272.7 bits (696), Expect = 1.9e-69
Identity = 134/148 (90.54%), Postives = 142/148 (95.95%), Query Frame = 0


Query: 6   SRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRAALPHL 65
           S +A+ IR+LQDAK+SSGKSYNQIAEETGLTNVYVAQLLRRQAHLK +TVPKLR+ALP L
Sbjct: 4   SSKATAIRQLQDAKRSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKPETVPKLRSALPQL 63

Query: 66  THEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSAIDFYC 125
           T +HIQEMMRPPMR YDPCLIQEPTIYRLNEAVMHFGESIKEIINE+FGDGIMSAIDFYC
Sbjct: 64  TDDHIQEMMRPPMRSYDPCLIQEPTIYRLNEAVMHFGESIKEIINEEFGDGIMSAIDFYC 123

Query: 126 SVDKVKGVDGKDRVVITFDGKYLPYTEQ 154
           SVDKVKGVDGKDRVVITFDGKYLPYTEQ
Sbjct: 124 SVDKVKGVDGKDRVVITFDGKYLPYTEQ 151

[Close]

BLAST of MS010418 vs. NCBI nr
Match: XP_023527332.1 (cyanate hydratase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 271.9 bits (694), Expect = 3.3e-69
Identity = 137/153 (89.54%), Postives = 143/153 (93.46%), Query Frame = 0


Query: 1   MEGSSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRA 60
           MEGSS  RAS IR+LQDAK+ SGKSYNQIAEETGLTNVYVAQLLRRQA LK +TVPKLRA
Sbjct: 1   MEGSS--RASAIRQLQDAKRLSGKSYNQIAEETGLTNVYVAQLLRRQAQLKPETVPKLRA 60

Query: 61  ALPHLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSA 120
           ALP L  +HIQEMM PPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEI+NE+FGDGIMSA
Sbjct: 61  ALPQLLDDHIQEMMLPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIVNEEFGDGIMSA 120

Query: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 154
           IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ
Sbjct: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 151

[Close]

BLAST of MS010418 vs. NCBI nr
Match: XP_022924434.1 (cyanate hydratase [Cucurbita moschata] >KAG6582406.1 Cyanate hydratase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 270.0 bits (689), Expect = 1.3e-68
Identity = 136/153 (88.89%), Postives = 142/153 (92.81%), Query Frame = 0


Query: 1   MEGSSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRA 60
           MEG  SRRA  IR+LQDAK+ SGKSYNQIAEETGLTNVYVAQLLRRQA LK +TVPKLRA
Sbjct: 1   MEG--SRRAIAIRQLQDAKRLSGKSYNQIAEETGLTNVYVAQLLRRQAQLKPETVPKLRA 60

Query: 61  ALPHLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSA 120
           ALP L  +HIQEMM PPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEI+NE+FGDGIMSA
Sbjct: 61  ALPQLLDDHIQEMMLPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIVNEEFGDGIMSA 120

Query: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 154
           IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ
Sbjct: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 151

[Close]

BLAST of MS010418 vs. ExPASy Swiss-Prot
Match: A5BJL8 (Cyanate hydratase OS=Vitis vinifera OX=29760 GN=CYN PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 2.8e-63
Identity = 119/148 (80.41%), Postives = 133/148 (89.86%), Query Frame = 0


Query: 8   RASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRAALPHLTH 67
           ++S+   L   K++SGKSY +IAEETGLTNVYVAQLLRRQA LKADT PKL+AALP LT 
Sbjct: 5   KSSVASRLMSVKRTSGKSYGRIAEETGLTNVYVAQLLRRQAQLKADTAPKLQAALPELTD 64

Query: 68  EHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSAIDFYCSV 127
           E +QEMM+PP+R YDP LIQEPT+YRLNEAVMHFGESIKEIINE+FGDGIMSAIDFYCSV
Sbjct: 65  ELLQEMMKPPLRSYDPHLIQEPTVYRLNEAVMHFGESIKEIINEEFGDGIMSAIDFYCSV 124

Query: 128 DKVKGVDGKDRVVITFDGKYLPYTEQVS 156
           DKVKGVDGKDRVV+TFDGKYLP+TEQ S
Sbjct: 125 DKVKGVDGKDRVVLTFDGKYLPHTEQKS 152

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BLAST of MS010418 vs. ExPASy Swiss-Prot
Match: C5WYI7 (Cyanate hydratase OS=Sorghum bicolor OX=4558 GN=CYN PE=3 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 4.1e-62
Identity = 116/152 (76.32%), Postives = 134/152 (88.16%), Query Frame = 0


Query: 4   SSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRAALP 63
           +S  RA+++R L + K+ SGK++++IA ETGLTNVYVAQLLRRQAHLKADTVP LRAALP
Sbjct: 3   ASGERAAVVRRLMEVKEESGKTFSEIAAETGLTNVYVAQLLRRQAHLKADTVPALRAALP 62

Query: 64  HLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSAIDF 123
            LT E +Q MM+PP R Y+P ++QEP IYRLNEAVMHFGESIKEIINE+FGDGIMSAIDF
Sbjct: 63  TLTDELVQLMMQPPFRSYNPDIVQEPAIYRLNEAVMHFGESIKEIINEEFGDGIMSAIDF 122

Query: 124 YCSVDKVKGVDGKDRVVITFDGKYLPYTEQVS 156
           YCSVDKV+G DGKDRVV+TFDGKYLPYTEQ S
Sbjct: 123 YCSVDKVQGADGKDRVVVTFDGKYLPYTEQKS 154

[Close]

BLAST of MS010418 vs. ExPASy Swiss-Prot
Match: B7FKW7 (Cyanate hydratase OS=Medicago truncatula OX=3880 GN=CYN PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 5.3e-62
Identity = 114/149 (76.51%), Postives = 132/149 (88.59%), Query Frame = 0


Query: 5   SSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRAALPH 64
           +  +A+ + +LQ  K  SGKSYNQ+AEETGLTNVYVAQLLRRQAHLK +T PKL+AALP 
Sbjct: 2   AQNKANTVSQLQSLKNKSGKSYNQLAEETGLTNVYVAQLLRRQAHLKPETAPKLKAALPE 61

Query: 65  LTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSAIDFY 124
           L  E I EMM+PP+R YDP +IQ+PT+YRLNEAVMHFGESIKEIINE+FGDGIMSAIDFY
Sbjct: 62  LPEELIHEMMKPPLRSYDPNIIQDPTVYRLNEAVMHFGESIKEIINEEFGDGIMSAIDFY 121

Query: 125 CSVDKVKGVDGKDRVVITFDGKYLPYTEQ 154
           CSVDKV+GVDGKDRVV+TFDGKYLP++EQ
Sbjct: 122 CSVDKVQGVDGKDRVVLTFDGKYLPHSEQ 150

[Close]

BLAST of MS010418 vs. ExPASy Swiss-Prot
Match: B9HSR7 (Cyanate hydratase OS=Populus trichocarpa OX=3694 GN=CYN PE=3 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 2.6e-61
Identity = 116/148 (78.38%), Postives = 129/148 (87.16%), Query Frame = 0


Query: 8   RASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRAALPHLTH 67
           + ++I  L+  K++SGKS +QIAE+TGLTNVYVAQLLRRQA L  DT P LRAALP L  
Sbjct: 5   KTTIINRLEAVKRNSGKSCSQIAEQTGLTNVYVAQLLRRQAQLNPDTAPSLRAALPELPE 64

Query: 68  EHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSAIDFYCSV 127
           + +QEMMRPPMR YDP LIQEP +YRLNEAVMHFGESIKEIINE+FGDGIMSAIDFYCSV
Sbjct: 65  DLLQEMMRPPMRSYDPNLIQEPCVYRLNEAVMHFGESIKEIINEEFGDGIMSAIDFYCSV 124

Query: 128 DKVKGVDGKDRVVITFDGKYLPYTEQVS 156
           DKVKGVDGKDRVV+TFDGKYLPYTEQ S
Sbjct: 125 DKVKGVDGKDRVVVTFDGKYLPYTEQKS 152

[Close]

BLAST of MS010418 vs. ExPASy Swiss-Prot
Match: C6SXE1 (Cyanate hydratase OS=Glycine max OX=3847 GN=CYN PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 1.3e-60
Identity = 113/149 (75.84%), Postives = 129/149 (86.58%), Query Frame = 0


Query: 7   RRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRAALPHLT 66
           ++A ++ ELQ  K +SGKSYNQ+AEETGLTNVYVAQLLRRQA L   T P LRAALP L 
Sbjct: 4   KKAKIVAELQAVKHNSGKSYNQLAEETGLTNVYVAQLLRRQAQLNPQTAPLLRAALPDLP 63

Query: 67  HEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSAIDFYCS 126
            + + EMMRPP+R YDP LIQ+PT+YRLNEAVMHFGESIKEIINE+FGDG MSAIDFYCS
Sbjct: 64  QDLVPEMMRPPLRSYDPNLIQDPTVYRLNEAVMHFGESIKEIINEEFGDGTMSAIDFYCS 123

Query: 127 VDKVKGVDGKDRVVITFDGKYLPYTEQVS 156
           VDK+KGVDGKDRVV+TFDGKYLP++EQ S
Sbjct: 124 VDKIKGVDGKDRVVLTFDGKYLPHSEQKS 152

[Close]

BLAST of MS010418 vs. ExPASy TrEMBL
Match: A0A6J1DKK3 (Cyanate hydratase OS=Momordica charantia OX=3673 GN=LOC111020919 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 2.6e-80
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 1   MEGSSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRA 60
           MEGSSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRA
Sbjct: 1   MEGSSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRA 60

Query: 61  ALPHLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSA 120
           ALPHLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSA
Sbjct: 61  ALPHLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSA 120

Query: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 154
           IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ
Sbjct: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 153

[Close]

BLAST of MS010418 vs. ExPASy TrEMBL
Match: A0A6J1IWH1 (Cyanate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111479117 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.2e-70
Identity = 139/153 (90.85%), Postives = 144/153 (94.12%), Query Frame = 0


Query: 1   MEGSSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRA 60
           MEGSS  RAS IR+LQDAK+ SGKS NQIAEETGLTNVYVAQLLRRQA LK +TVPKLRA
Sbjct: 1   MEGSS--RASAIRQLQDAKRLSGKSCNQIAEETGLTNVYVAQLLRRQAQLKPETVPKLRA 60

Query: 61  ALPHLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSA 120
           ALPHLT +HIQEMM PPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINE+FGDGIMSA
Sbjct: 61  ALPHLTDDHIQEMMLPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEEFGDGIMSA 120

Query: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 154
           IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ
Sbjct: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 151

[Close]

BLAST of MS010418 vs. ExPASy TrEMBL
Match: A0A6J1E962 (Cyanate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111431938 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 6.1e-69
Identity = 136/153 (88.89%), Postives = 142/153 (92.81%), Query Frame = 0


Query: 1   MEGSSSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRA 60
           MEG  SRRA  IR+LQDAK+ SGKSYNQIAEETGLTNVYVAQLLRRQA LK +TVPKLRA
Sbjct: 1   MEG--SRRAIAIRQLQDAKRLSGKSYNQIAEETGLTNVYVAQLLRRQAQLKPETVPKLRA 60

Query: 61  ALPHLTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSA 120
           ALP L  +HIQEMM PPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEI+NE+FGDGIMSA
Sbjct: 61  ALPQLLDDHIQEMMLPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIVNEEFGDGIMSA 120

Query: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 154
           IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ
Sbjct: 121 IDFYCSVDKVKGVDGKDRVVITFDGKYLPYTEQ 151

[Close]

BLAST of MS010418 vs. ExPASy TrEMBL
Match: A0A1S3AX45 (Cyanate hydratase OS=Cucumis melo OX=3656 GN=LOC103483565 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 5.7e-67
Identity = 129/148 (87.16%), Postives = 140/148 (94.59%), Query Frame = 0


Query: 6   SRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRAALPHL 65
           S RA++IR+L +AK SSGKSYNQIA+ETGLTNVYVAQLLRRQA LK DTVPKLR+ALP L
Sbjct: 4   SSRATVIRQLHNAKCSSGKSYNQIAQETGLTNVYVAQLLRRQAQLKPDTVPKLRSALPQL 63

Query: 66  THEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSAIDFYC 125
           T +HIQ+MMRPPMR YDPCLIQEPT+YRLNEAVMHFGESIKEIINE+FGDGIMSAIDFYC
Sbjct: 64  TDDHIQQMMRPPMRSYDPCLIQEPTVYRLNEAVMHFGESIKEIINEEFGDGIMSAIDFYC 123

Query: 126 SVDKVKGVDGKDRVVITFDGKYLPYTEQ 154
           SVDKV+GVDGKDRVVITFDGKYLPYTEQ
Sbjct: 124 SVDKVQGVDGKDRVVITFDGKYLPYTEQ 151

[Close]

BLAST of MS010418 vs. ExPASy TrEMBL
Match: A0A0A0L9Q7 (Cyanate hydratase OS=Cucumis sativus OX=3659 GN=CYN PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 9.7e-67
Identity = 129/148 (87.16%), Postives = 140/148 (94.59%), Query Frame = 0


Query: 6   SRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRAALPHL 65
           S RA+++R+LQ+AK SSGKSYNQIA+ETGLTNVYVAQLLR QA LK DTVPKLR+ALP L
Sbjct: 4   SSRATVVRQLQNAKCSSGKSYNQIAQETGLTNVYVAQLLRGQAQLKPDTVPKLRSALPQL 63

Query: 66  THEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSAIDFYC 125
           T +HIQ+MMRPPMR YDPCLIQEPTIYRLNEAVMHFGESIKEIINE+FGDGIMSAIDFYC
Sbjct: 64  TDDHIQQMMRPPMRSYDPCLIQEPTIYRLNEAVMHFGESIKEIINEEFGDGIMSAIDFYC 123

Query: 126 SVDKVKGVDGKDRVVITFDGKYLPYTEQ 154
           SVDKVKGVDGKDRVVITFDGKYLPY+EQ
Sbjct: 124 SVDKVKGVDGKDRVVITFDGKYLPYSEQ 151

[Close]

BLAST of MS010418 vs. TAIR 10
Match: AT3G23490.1 (cyanase )

HSP 1 Score: 219.9 bits (559), Expect = 1.4e-57
Identity = 109/149 (73.15%), Postives = 127/149 (85.23%), Query Frame = 0


Query: 5   SSRRASLIRELQDAKQSSGKSYNQIAEETGLTNVYVAQLLRRQAHLKADTVPKLRAALPH 64
           ++++ S+  +L   K +SGK+++Q+A ETGLTNVYVAQLLRRQA LK DTVPKL+ ALP 
Sbjct: 3   AAKKQSVTNQLLAVKSASGKTFSQLAAETGLTNVYVAQLLRRQAQLKPDTVPKLKEALPA 62

Query: 65  LTHEHIQEMMRPPMRCYDPCLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSAIDFY 124
           LT E I +MM PP R YDP LIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSAIDFY
Sbjct: 63  LTDELIGDMMSPPWRSYDPNLIQEPTIYRLNEAVMHFGESIKEIINEDFGDGIMSAIDFY 122

Query: 125 CSVDKVKGVDGKDRVVITFDGKYLPYTEQ 154
           CSVDK+KGVDG +RVV+T DGKYL ++EQ
Sbjct: 123 CSVDKIKGVDGNNRVVVTLDGKYLSHSEQ 151

The following BLAST results are available for this feature:

BLAST of MS010418 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153411.1	5.5e-80	100.00	cyanate hydratase [Momordica charantia]	[more]
XP_022979374.1	6.7e-70	90.85	cyanate hydratase [Cucurbita maxima]	[more]
XP_038879847.1	1.9e-69	90.54	cyanate hydratase [Benincasa hispida]	[more]
XP_023527332.1	3.3e-69	89.54	cyanate hydratase [Cucurbita pepo subsp. pepo]	[more]
XP_022924434.1	1.3e-68	88.89	cyanate hydratase [Cucurbita moschata] >KAG6582406.1 Cyanate hydratase, partial ...	[more]

BLAST of MS010418 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A5BJL8	2.8e-63	80.41	Cyanate hydratase OS=Vitis vinifera OX=29760 GN=CYN PE=3 SV=1	[more]
C5WYI7	4.1e-62	76.32	Cyanate hydratase OS=Sorghum bicolor OX=4558 GN=CYN PE=3 SV=1	[more]
B7FKW7	5.3e-62	76.51	Cyanate hydratase OS=Medicago truncatula OX=3880 GN=CYN PE=2 SV=1	[more]
B9HSR7	2.6e-61	78.38	Cyanate hydratase OS=Populus trichocarpa OX=3694 GN=CYN PE=3 SV=1	[more]
C6SXE1	1.3e-60	75.84	Cyanate hydratase OS=Glycine max OX=3847 GN=CYN PE=2 SV=1	[more]

BLAST of MS010418 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DKK3	2.6e-80	100.00	Cyanate hydratase OS=Momordica charantia OX=3673 GN=LOC111020919 PE=3 SV=1	[more]
A0A6J1IWH1	3.2e-70	90.85	Cyanate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111479117 PE=3 SV=1	[more]
A0A6J1E962	6.1e-69	88.89	Cyanate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111431938 PE=3 SV=1	[more]
A0A1S3AX45	5.7e-67	87.16	Cyanate hydratase OS=Cucumis melo OX=3656 GN=LOC103483565 PE=3 SV=1	[more]
A0A0A0L9Q7	9.7e-67	87.16	Cyanate hydratase OS=Cucumis sativus OX=3659 GN=CYN PE=3 SV=1	[more]

BLAST of MS010418 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23490.1	1.4e-57	73.15	cyanase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008076	Cyanate hydratase	PRINTS	PR01693	CYANASE	coord: 106..122 score: 77.31 coord: 138..148 score: 72.73 coord: 39..56 score: 42.06 coord: 19..34 score: 33.93 coord: 88..102 score: 54.29
IPR008076	Cyanate hydratase	TIGRFAM	TIGR00673	TIGR00673	coord: 10..151 e-value: 1.6E-35 score: 120.3
IPR008076	Cyanate hydratase	PIRSF	PIRSF001263	Cyanate_hydratas	coord: 1..153 e-value: 1.2E-62 score: 208.3
IPR008076	Cyanate hydratase	PANTHER	PTHR34186	CYANATE HYDRATASE	coord: 5..154
IPR008076	Cyanate hydratase	HAMAP	MF_00535	Cyanate_hydrat	coord: 1..155 score: 51.196815
IPR003712	Cyanate lyase, C-terminal	SMART	SM01116	Cyanate_lyase_2	coord: 80..153 e-value: 9.5E-42 score: 154.7
IPR003712	Cyanate lyase, C-terminal	PFAM	PF02560	Cyanate_lyase	coord: 85..151 e-value: 7.2E-31 score: 105.9
IPR003712	Cyanate lyase, C-terminal	CDD	cd00559	Cyanase_C	coord: 87..153 e-value: 4.56032E-39 score: 124.956
IPR010982	Lambda repressor-like, DNA-binding domain superfamily	GENE3D	1.10.260.40		coord: 2..84 e-value: 4.6E-12 score: 47.5
IPR010982	Lambda repressor-like, DNA-binding domain superfamily	SUPERFAMILY	47413	lambda repressor-like DNA-binding domains	coord: 8..83
IPR036581	Cyanate lyase, C-terminal domain superfamily	GENE3D	3.30.1160.10		coord: 87..153 e-value: 1.7E-32 score: 112.9
IPR036581	Cyanate lyase, C-terminal domain superfamily	SUPERFAMILY	55234	Cyanase C-terminal domain	coord: 87..152
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010418.1	MS010418.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009439	cyanate metabolic process
molecular_function	GO:0008824	cyanate hydratase activity
molecular_function	GO:0003677	DNA binding