MS010205 (gene) Bitter gourd (TR) v1

Overview


Name	MS010205
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	E3 ubiquitin ligase BIG BROTHER-like
Location	scaffold391: 672142 .. 676910 (-)
RNA-Seq Expression	MS010205
Synteny	MS010205

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCTGGAACACGAGCATGGAGGTTAATTGCATGAATAATAGTTACCCTTACAGCACAGCTGGAAGCTTTATGGAATACTTTGAAGGTCTTACATATGAACACGTGAATTTCATTTTTTCCGGTGCCTCACATTCTCAGGTACTGTACATCTCTTTCCTTTCGATGCCTTCCTTGGATCTCGTCTGTGACTAGGTTGATAGAACTCATGGCATTTAGATGCTGTTTTGTCTCTGTACCTGCTACTCAAAGGAAGTTATTTGGCGTTACTTGTTGCCATGAGATCTCTACTGTTTCTTTGCTCTGTTGGTTGTTATCTTGGATAATCTCCCTATCTCTTTCAAAGTTGCTTCCCTCCTCTTAAGACTCCTTGAAAATTGAATCTCCCCCTACCCAAATATAGAAAAAGGAAAGCAAACCACATTTCATCTTCAAAGTAGTTACTGCTTGGAAGCTTATGGTTTCATGTTCTATTTCACTCCTGGGATATAGCCCTTTATCATGCTTAAACTTCCTGATGCATGCTTGATTGGACATCTATTATCGAGATCGAATAGTATTTATGTCTAGTTTTCATACTTCCTCATGCATTAAATGAATTTCGCAACATGGGAGTATAATATTTTCGTTCTTCCATTCAGGAGACTGTTTATCCATCCACTAATTCAAGTTACTATAAGTTTGGGCATTCTGATTCTTGGAGCAGTTCATACTTTGATGCTCAATCATCTGAGGTACTAGGTCATGAGTCCACTGTTGATGAACATAGGAGGCCGCAGGACTTCTCGACATTACCGAATGAACAGAGTTCAGGAAATAGAGCGTGGGAAGAAAATGCCAATCCCAATATGTCCAGCAACACCAACACCATGGAATGTAAGGATTGAGTTCTGTGTCATTTCAACTTTCCATGAAACAGAATTTAGATATTAAGTGCTAATGTTCTATAAAACCACATGTTGTGAAGAGTATAATTAATGACTCTCTGTTTTGATTTTTAGTCTCTTATTTTTTCTCTGTTCCTGAATTGAGGTATTTGTTGAGAAAAAGCAGTTGGTGTCATCAATTTTCAGGCATTGGCAACCTTCATCTTCATGATCGCTATTGAGGGCATTCTTTTTGCTAATATAGTGCATTTTTGGCATATTTGAAAACTATAATGGTTATATCGGTCTATGGTTTTCTTATGACACAGATATCAAATGAAAGTTAAATGTTGCCGAAAAATTGGCTTTAGGCATATTAGTTATTTACTGGGATGACTTATTCGATGCAACAAATTGAATGTAGGGTGCTTCAACGAGTATCTTGTAAATGAGATACATTCACCATCCATTGATATACATGTTCTACCATATTTCAATGTTAACTTGCAACCACTGGGTGAACCACTTTATATTTAGCAAGTACTATCCGGAGTAGAATTAACTTGCTGCTAGGTAGAGTGTTAAAGGTATATATGAAATCTAACAACCAAAATTTTTCCTCTTGGCTTCCTTCTGATCCAATTAAAATGTTACTGAAAATTTTTTTCTAGTTCCCAACTTTAACTATTCTCCATTGCTTTTTCAGGCCCACGTAGGCATCCAAATTATCAAGAGTATCAGGTCTGCAGCTTTTTTATTCTTAAATTTTTTGTGCCATTAAGTCTACAGTTGCAGTTTTATTGTAAGGTTGTTGACAATAGATACCTCACTTTTTGGCTTAGCATCCCTGTATTTATTTGGGTATGTTATAAAACTGGCACATATAAACTGAAGTAGAGAGAGAATACAGAATAGGAATTCATTTTCTAACCAATGTTGACAACTTGACAGCCTAGGTGGTGGTTCAGGTTTCCCTAATAATCTTTGTCCCAAGAGTTTTTGTTCAAGGCCATATAGACCTGGAATTTACTTCCAAACTTCTTCAGAAAAGATTTTATTGAATGCGCTCCCTAATCAGTTCCACTTTGAAATATGCAGACTATTTGGCAAGATATTGTTGATCCAGATAACATGACTTATGAGGTTAGAGCTGATAATAATTTTCTTTCCCTCTTAATTTTGCAAATTGCCGCACAAAATTTTCCTCTCCTGTAAATTTACATGTTCTTATCGAAGTTAAGTTTTCTTACTCAATGCCTAATTGTTGAATTTTGTAATTGAATTGTTATTATGGTTACTATTGTTGAAATACAACACTAACTATCTAGAAGCTTCTTGGAAGTTTCTACACATTTCTCTTATAGGTAATAGTTAGTCTTTCTAGATTGTTCTTGAAGATTGTAAATAAATATCTAAAGAAAAGGATCTATAAATATCTTAGACTTTCTCTTAGGTTCCAAAGGATTCTAGAGAAATCTTTTGGATTAGAATGGATAAGGAAAAATCTAGAATATGTAAAATTTGAGAGGAGTCTAGAAAGTTATTTTTGCTTGGGCTCCAAGGATCTTTCTCCTATAAATAGGAGAGATGTCCATGTGAATCAAGTGAATAGAGTGATGAGAGTGAACCAAGTGAAATGAGTGAAGAAAGAGAGAAAAGTTAGAAAGTGAGAGAGTCTTCCATCAAAGAGTGTCCATATTCATACTTTCTAAATAAAAATTTCTTTCTTCAAGTGATTGTTTATTTCCTTTTGTGCTAATTCATTCAACAAGTGGTATCAGAGTTGCGCTAATTAATTCAACAAGTATGAAGTCCTTGCTGCTGTAAAAGCCAATGGCAAAACTAGTTGCCATTGCAGCTACTAAGCTGAAATTGGCAGTAGGCTTGGTTTTTGATGCTTTCCTTACCAACGTAGAAACGCAAGAAATGCTGGTTCAGATTAATCTAGTTGTTTGTTCTGCATTGTGGTTGCTGGTACACTTCCAGCTAGTTTTCTAACCTTGAGCCTTGCTTCTTCGGAGGATCTTTTAAAAACATAAACTTGCTTATTTGGATGGATCTGCCTGCTGATAGTGCTTTTAAGTACCTGACATTTATTAGTTATGAGGATAAGGAACTCATGTGGCAGCTGTAAATCCAGTTGGTACTTCAAGGCCTATAACTAATAAACGTAAAAGTTTGTCTGGCTAACTGTAATGTGTAAACTCCTGTGTATGCCTCCTAAATTATGTCGCTTACTCTCTTTAATATGATAATGGTTTAAAGCAACTAAATTTCAGGAATTACTAGATTTAGGCGAGACAGTTGGAACTCATAATCGAGGCCTTCCACAAGAACTGATTGCGTTGCTTCCAGTATCAAAGTATAAATGTGGGTTTTTCTTGATGAAGAAATCACGAAATGAAAGGTAACACTTGATTGATTGATTGTCTTATACCATATGATTTTTTCAAACTTTTATTGTTTCTGCTTTGTTTTTTAGGATTTTTTCCAGCTATGAAGATAATTGAATATAAACCCTGCAATTCCTGCAATTGGATTTTTTCCAGCTATGAAGATAATTGAATATAAACCCTGCAATTCCTGCAATTGGATTTTTTCCAGCTATGAAGATAATTGAATATAAACCCTGCAATTCCTGCAATTCACTTTTGAAATTTGAAATGTTTAACTTCACTGTTTTCTATCTTTGTGCATGCTGCAAAGTTAAACTATTTTGTACTGTTTTGAATAGTGTTTCTCCATGTTTTTGGATATATGTTAGCGAGCAATTTGATAGAACCCAAAAGAACTATAATACTATTGAACTACAATAACTCTGTATTCAAGCTACTTGAAATCTCTCCTCTTTGAGTATTTCCACTCAAATCCAAACCAAACAAAACAATTGCTTACCTCCATCATTTCCTACGCACTCTATTTATAACAAATCTCCAGCACAATTTCTGTAACTAATTATTAGTATACCACTAATGCTCTAATAATATTCCTAATTCTATCTTGATATCACAATTATGTTGCTAGCTAACTAAAAATAAAAAGCTGGCTGAAAGTGGTAGTCGTATATGATTTACTGGGTCAATTGACTTTTTGGTCAGATGCTGGACATTTAAGAGTAAAACTAAGGACGTTATAAAAATTTAAATGCATGTAGAGTTGTCCTACAAAGTTGTCAGCACTCTACGTGGAACTACGGTGGTATGTACTATTGAAGAATAAAGCTTCTATGTGTCTACTTACCAATACCCTTATATATATAGCTTAATCATGTCCATGAGTTCTTATATTCTCATTCCCACCATTAACGTTGAAATTAATCTATAGTTATGTTAATGATCTTTCTCACAACAATCTAATTATAGAAAGAAAACACTTACAGAGTGTTTTGGCTGAGTAGAGTAGCCTACGTCTGCCATTCACACCAGTACCTTTAATTCAATTACGAAGAATACAATGATCAGAAGACCCAAGAGGTGCATAAGTTTTGCTCAACACTTCAGAGAGAACTCAAAAGCTTGAACCCTTTTCACTCAAAAACATTTTTGAGTCATAAAGACTACGCTATATAAAATATGTCTTCCTTTGGAAATTATTTTTTGACAAGAATGCATCTGACTACATAAATCAGTCTTCATTTGTATATAGGTGTGTGATATGCCAGATGGAATACAAACGTGGAGATCAGAGGATCACTCTACCGTGCAAACACAGGTACCATACCTGTTGCGGGACTAAGTGGCTCAGTATCAACAAGGTGACGTTCTGATATTTTTTATCCCGGCACATTTTGAGGATAATTTTAGTAACGGGCCATAAATGACTTTAGCTAATACATTACGTAATTCTCTGTTCCAGGCTTGTCCCATTTGCTACACTGAAGTTTTCGGTGGCACTTCAAAGCGT

mRNA sequence

ATGAGCTGGAACACGAGCATGGAGGTTAATTGCATGAATAATAGTTACCCTTACAGCACAGCTGGAAGCTTTATGGAATACTTTGAAGGTCTTACATATGAACACGTGAATTTCATTTTTTCCGGTGCCTCACATTCTCAGGAGACTGTTTATCCATCCACTAATTCAAGTTACTATAAGTTTGGGCATTCTGATTCTTGGAGCAGTTCATACTTTGATGCTCAATCATCTGAGGTACTAGGTCATGAGTCCACTGTTGATGAACATAGGAGGCCGCAGGACTTCTCGACATTACCGAATGAACAGAGTTCAGGAAATAGAGCGTGGGAAGAAAATGCCAATCCCAATATGTCCAGCAACACCAACACCATGGAATGCCCACGTAGGCATCCAAATTATCAAGAGTATCAGACTATTTGGCAAGATATTGTTGATCCAGATAACATGACTTATGAGCCAATGGCAAAACTAGTTGCCATTGCAGCTACTAAGCTGAAATTGGCAGTAGGCTTGGTTTTTGATGCTTTCCTTACCAACGTAGAAACGCAAGAAATGCTGGTTCAGATTAATCTAGAATTACTAGATTTAGGCGAGACAGTTGGAACTCATAATCGAGGCCTTCCACAAGAACTGATTGCGTTGCTTCCAGTATCAAAGTATAAATGTGGGTTTTTCTTGATGAAGAAATCACGAAATGAAAGGTGTGTGATATGCCAGATGGAATACAAACGTGGAGATCAGAGGATCACTCTACCGTGCAAACACAGGTACCATACCTGTTGCGGGACTAAGTGGCTCAGTATCAACAAGGCTTGTCCCATTTGCTACACTGAAGTTTTCGGTGGCACTTCAAAGCGT

Coding sequence (CDS)

ATGAGCTGGAACACGAGCATGGAGGTTAATTGCATGAATAATAGTTACCCTTACAGCACAGCTGGAAGCTTTATGGAATACTTTGAAGGTCTTACATATGAACACGTGAATTTCATTTTTTCCGGTGCCTCACATTCTCAGGAGACTGTTTATCCATCCACTAATTCAAGTTACTATAAGTTTGGGCATTCTGATTCTTGGAGCAGTTCATACTTTGATGCTCAATCATCTGAGGTACTAGGTCATGAGTCCACTGTTGATGAACATAGGAGGCCGCAGGACTTCTCGACATTACCGAATGAACAGAGTTCAGGAAATAGAGCGTGGGAAGAAAATGCCAATCCCAATATGTCCAGCAACACCAACACCATGGAATGCCCACGTAGGCATCCAAATTATCAAGAGTATCAGACTATTTGGCAAGATATTGTTGATCCAGATAACATGACTTATGAGCCAATGGCAAAACTAGTTGCCATTGCAGCTACTAAGCTGAAATTGGCAGTAGGCTTGGTTTTTGATGCTTTCCTTACCAACGTAGAAACGCAAGAAATGCTGGTTCAGATTAATCTAGAATTACTAGATTTAGGCGAGACAGTTGGAACTCATAATCGAGGCCTTCCACAAGAACTGATTGCGTTGCTTCCAGTATCAAAGTATAAATGTGGGTTTTTCTTGATGAAGAAATCACGAAATGAAAGGTGTGTGATATGCCAGATGGAATACAAACGTGGAGATCAGAGGATCACTCTACCGTGCAAACACAGGTACCATACCTGTTGCGGGACTAAGTGGCTCAGTATCAACAAGGCTTGTCCCATTTGCTACACTGAAGTTTTCGGTGGCACTTCAAAGCGT

Protein sequence

MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYKFGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSNTNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNVETQEMLVQINLELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQMEYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR

Homology

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BLAST of MS010205 vs. NCBI nr
Match: XP_022148861.1 (E3 ubiquitin ligase BIG BROTHER-like isoform X2 [Momordica charantia])

HSP 1 Score: 503.1 bits (1294), Expect = 1.6e-138
Identity = 247/286 (86.36%), Postives = 247/286 (86.36%), Query Frame = 0


Query: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 60
           MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK
Sbjct: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 60

Query: 61  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 120
           FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN
Sbjct: 61  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 120

Query: 121 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNV 180
           TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYE                            
Sbjct: 121 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYE---------------------------- 180

Query: 181 ETQEMLVQINLELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 240
                      ELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM
Sbjct: 181 -----------ELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 240

Query: 241 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 287
           EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR
Sbjct: 241 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 247

[Close]

BLAST of MS010205 vs. NCBI nr
Match: XP_022148859.1 (E3 ubiquitin ligase BIG BROTHER-like isoform X1 [Momordica charantia] >XP_022148860.1 E3 ubiquitin ligase BIG BROTHER-like isoform X1 [Momordica charantia])

HSP 1 Score: 503.1 bits (1294), Expect = 1.6e-138
Identity = 247/286 (86.36%), Postives = 247/286 (86.36%), Query Frame = 0


Query: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 60
           MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK
Sbjct: 13  MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 72

Query: 61  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 120
           FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN
Sbjct: 73  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 132

Query: 121 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNV 180
           TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYE                            
Sbjct: 133 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYE---------------------------- 192

Query: 181 ETQEMLVQINLELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 240
                      ELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM
Sbjct: 193 -----------ELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 252

Query: 241 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 287
           EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR
Sbjct: 253 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 259

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BLAST of MS010205 vs. NCBI nr
Match: XP_038901510.1 (E3 ubiquitin-protein ligase BIG BROTHER-like [Benincasa hispida])

HSP 1 Score: 417.2 bits (1071), Expect = 1.2e-112
Identity = 210/286 (73.43%), Postives = 224/286 (78.32%), Query Frame = 0


Query: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 60
           MSWN+++EV+CMNN YPYSTA SFMEYFEGLTY+HVNFIFSGASHSQET+YP TNSSYYK
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTA-SFMEYFEGLTYDHVNFIFSGASHSQETIYPLTNSSYYK 60

Query: 61  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 120
           FGHSDSWS+S+FDAQS E+ GHEST+DEHRR    ST PNEQS GNR WEENANPNMSS 
Sbjct: 61  FGHSDSWSTSHFDAQSFEIQGHESTIDEHRRL--LSTAPNEQSLGNRVWEENANPNMSS- 120

Query: 121 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNV 180
            N MECPRRH NY EYQTIWQDIVDPDNMTYE                            
Sbjct: 121 -NNMECPRRHSNYHEYQTIWQDIVDPDNMTYE---------------------------- 180

Query: 181 ETQEMLVQINLELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 240
                      EL+DLGETVGT +RGL QELI+LLPVSKYKCGFFL  KSRNERCVICQM
Sbjct: 181 -----------ELVDLGETVGTQSRGLSQELISLLPVSKYKCGFFLRNKSRNERCVICQM 240

Query: 241 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 287
           EYKRGDQRITLPCKHRYHT CGTKWLSINKACPICYTEVFG +SKR
Sbjct: 241 EYKRGDQRITLPCKHRYHTSCGTKWLSINKACPICYTEVFGDSSKR 242

[Close]

BLAST of MS010205 vs. NCBI nr
Match: XP_004142225.1 (E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >XP_011653590.1 E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >XP_031739641.1 E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >XP_031739642.1 E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >KAE8649510.1 hypothetical protein Csa_017925 [Cucumis sativus])

HSP 1 Score: 393.3 bits (1009), Expect = 1.8e-105
Identity = 203/286 (70.98%), Postives = 219/286 (76.57%), Query Frame = 0


Query: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 60
           MSWN+++EV+CMNN YPYSTA SFMEYFEGLTY+HVNFIFSGASHSQETV PSTNSS YK
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTA-SFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSS-YK 60

Query: 61  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 120
           FGHSD WS+SYFDAQS EV  HEST+DEHRRP   ST+ NEQSSGN  WEENANPNMS  
Sbjct: 61  FGHSDLWSASYFDAQSFEVQDHESTIDEHRRP--LSTVQNEQSSGNSVWEENANPNMSG- 120

Query: 121 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNV 180
            N+MECPRRH NY EYQTIWQ+IVDPDNMTYE                            
Sbjct: 121 -NSMECPRRHSNYHEYQTIWQEIVDPDNMTYE---------------------------- 180

Query: 181 ETQEMLVQINLELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 240
                      EL+DLGETVGT +RGL QELIALLPVS+Y+ GFF  KKSRNERCVICQM
Sbjct: 181 -----------ELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQM 240

Query: 241 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 287
           EYKRGD+ ITLPCKH+YHT CGTKWLSI KACPICYTEVFG TSKR
Sbjct: 241 EYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDTSKR 241

[Close]

BLAST of MS010205 vs. NCBI nr
Match: XP_008449725.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X1 [Cucumis melo])

HSP 1 Score: 388.3 bits (996), Expect = 5.9e-104
Identity = 200/286 (69.93%), Postives = 215/286 (75.17%), Query Frame = 0


Query: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 60
           MSWN+++EV+CMNN YPYSTA SFMEYFEGLTY+HVNFIFSGASHSQ+TV PSTNSSYYK
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTA-SFMEYFEGLTYDHVNFIFSGASHSQDTVCPSTNSSYYK 60

Query: 61  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 120
           FG+SD WS+SYFDAQ  EV  HES + EHRRP   ST+ NEQS GN  WEENANPNMS  
Sbjct: 61  FGNSDLWSTSYFDAQLFEVQDHESAIYEHRRP--LSTVQNEQSLGNSVWEENANPNMSG- 120

Query: 121 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNV 180
            N+MECPRRH NY EYQTIWQDIVDPDNMTYE                            
Sbjct: 121 -NSMECPRRHSNYHEYQTIWQDIVDPDNMTYE---------------------------- 180

Query: 181 ETQEMLVQINLELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 240
                      EL+DLGETVGT +RGL QELIALLPVS Y+ GFF  KKSRNERCVICQM
Sbjct: 181 -----------ELVDLGETVGTQSRGLTQELIALLPVSSYRWGFFSRKKSRNERCVICQM 240

Query: 241 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 287
           EYKRGD+ ITLPCKHRYHT CGTKWLSI KACPICYTEVFG TSKR
Sbjct: 241 EYKRGDKIITLPCKHRYHTSCGTKWLSIKKACPICYTEVFGDTSKR 242

[Close]

BLAST of MS010205 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 6.4e-37
Identity = 92/270 (34.07%), Postives = 135/270 (50.00%), Query Frame = 0


Query: 16  YPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYKFGHSDSWSSSYFDAQ 75
           +PY+  GS+M+++ G     +N+  +   H Q+ +Y + N++ YKFG S S ++S++   
Sbjct: 18  FPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNAYKFGFSGSDNASFYG-- 77

Query: 76  SSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENAN-PNMSSNTNTMECPRRHPNYQ 135
           S ++  H S +   R   D+  + N           +    +   ++   EC     +  
Sbjct: 78  SYDMNDHLSRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDTDEHSEAEECIANEHDPD 137

Query: 136 EYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNVETQEMLVQINLELL 195
             Q  WQD +DPD MTYE                                       EL+
Sbjct: 138 SPQVSWQDDIDPDTMTYE---------------------------------------ELV 197

Query: 196 DLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQMEYKRGDQRITLPCK 255
           +LGE VGT +RGL QELI  LP  KYK G    +K   ERCVICQ++YK G++++ LPCK
Sbjct: 198 ELGEAVGTESRGLSQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCK 246

Query: 256 HRYHTCCGTKWLSINKACPICYTEVFGGTS 285
           H YH+ C +KWLSINK CP+C +EVFG  S
Sbjct: 258 HVYHSECISKWLSINKVCPVCNSEVFGEPS 246

[Close]

BLAST of MS010205 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.5e-19
Identity = 46/93 (49.46%), Postives = 58/93 (62.37%), Query Frame = 0


Query: 192 ELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQMEYKRGDQRITL 251
           ELL LG+ VGT +RGL  + IA LP  +YK G    +   NE CVIC+++Y+  +  I L
Sbjct: 247 ELLALGDIVGTESRGLSADTIASLPSKRYKEGD--NQNGTNESCVICRLDYEDDEDLILL 306

Query: 252 PCKHRYHTCCGTKWLSINKACPICYTEVFGGTS 285
           PCKH YH+ C   WL INK CP+C  EV   TS
Sbjct: 307 PCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

[Close]

BLAST of MS010205 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.3e-10
Identity = 33/86 (38.37%), Postives = 42/86 (48.84%), Query Frame = 0


Query: 192 ELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQMEYKRGDQRITL 251
           ELL LGE +G  + GL +E+I  +                 E C +CQ EY  GD   TL
Sbjct: 576 ELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAGSHQDMEPCCVCQEEYAEGDDLGTL 635

Query: 252 PCKHRYHTCCGTKWLSINKACPICYT 278
            C H +HT C  +WL +   CPIC T
Sbjct: 636 GCGHEFHTACVKQWLMLKNLCPICKT 661

[Close]

BLAST of MS010205 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 2.5e-09
Identity = 32/84 (38.10%), Postives = 41/84 (48.81%), Query Frame = 0


Query: 192 ELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQMEYKRGDQRITL 251
           ELL L E +G  + GL +E +  L   +    + L      E C ICQ EY  GD   TL
Sbjct: 577 ELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQEEYVDGDDLGTL 636

Query: 252 PCKHRYHTCCGTKWLSINKACPIC 276
            C H +H  C  +WL +   CPIC
Sbjct: 637 DCGHDFHVGCVRQWLVVKNTCPIC 660

[Close]

BLAST of MS010205 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.5e-09
Identity = 36/91 (39.56%), Postives = 47/91 (51.65%), Query Frame = 0


Query: 192 ELLDLGETVGTHNRGLPQELIALLPVSKYK----CGFFLMKKSRNERCVICQMEYKRGDQ 251
           ELL LGE +G  + GL +E+I L  + ++K        +      E C ICQ EY  GD 
Sbjct: 610 ELLALGERIGDVSTGLSEEVI-LKAMKQHKHTSSSPSSVELHQNIEPCCICQEEYVEGDN 669

Query: 252 RITLPCKHRYHTCCGTKWLSINKACPICYTE 279
             TL C H +H  C  +W+ I   CPIC TE
Sbjct: 670 LGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

[Close]

BLAST of MS010205 vs. ExPASy TrEMBL
Match: A0A6J1D445 (E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017416 PE=4 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 7.9e-139
Identity = 247/286 (86.36%), Postives = 247/286 (86.36%), Query Frame = 0


Query: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 60
           MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK
Sbjct: 13  MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 72

Query: 61  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 120
           FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN
Sbjct: 73  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 132

Query: 121 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNV 180
           TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYE                            
Sbjct: 133 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYE---------------------------- 192

Query: 181 ETQEMLVQINLELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 240
                      ELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM
Sbjct: 193 -----------ELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 252

Query: 241 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 287
           EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR
Sbjct: 253 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 259

[Close]

BLAST of MS010205 vs. ExPASy TrEMBL
Match: A0A6J1D6M7 (E3 ubiquitin ligase BIG BROTHER-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017416 PE=4 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 7.9e-139
Identity = 247/286 (86.36%), Postives = 247/286 (86.36%), Query Frame = 0


Query: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 60
           MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK
Sbjct: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 60

Query: 61  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 120
           FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN
Sbjct: 61  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 120

Query: 121 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNV 180
           TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYE                            
Sbjct: 121 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYE---------------------------- 180

Query: 181 ETQEMLVQINLELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 240
                      ELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM
Sbjct: 181 -----------ELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 240

Query: 241 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 287
           EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR
Sbjct: 241 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 247

[Close]

BLAST of MS010205 vs. ExPASy TrEMBL
Match: A0A0A0L1Z5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G290860 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 8.8e-106
Identity = 203/286 (70.98%), Postives = 219/286 (76.57%), Query Frame = 0


Query: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 60
           MSWN+++EV+CMNN YPYSTA SFMEYFEGLTY+HVNFIFSGASHSQETV PSTNSS YK
Sbjct: 9   MSWNSNIEVHCMNNGYPYSTA-SFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSS-YK 68

Query: 61  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 120
           FGHSD WS+SYFDAQS EV  HEST+DEHRRP   ST+ NEQSSGN  WEENANPNMS  
Sbjct: 69  FGHSDLWSASYFDAQSFEVQDHESTIDEHRRP--LSTVQNEQSSGNSVWEENANPNMSG- 128

Query: 121 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNV 180
            N+MECPRRH NY EYQTIWQ+IVDPDNMTYE                            
Sbjct: 129 -NSMECPRRHSNYHEYQTIWQEIVDPDNMTYE---------------------------- 188

Query: 181 ETQEMLVQINLELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 240
                      EL+DLGETVGT +RGL QELIALLPVS+Y+ GFF  KKSRNERCVICQM
Sbjct: 189 -----------ELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQM 248

Query: 241 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 287
           EYKRGD+ ITLPCKH+YHT CGTKWLSI KACPICYTEVFG TSKR
Sbjct: 249 EYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDTSKR 249

[Close]

BLAST of MS010205 vs. ExPASy TrEMBL
Match: A0A1S3BNC2 (E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491520 PE=4 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.8e-104
Identity = 200/286 (69.93%), Postives = 215/286 (75.17%), Query Frame = 0


Query: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 60
           MSWN+++EV+CMNN YPYSTA SFMEYFEGLTY+HVNFIFSGASHSQ+TV PSTNSSYYK
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTA-SFMEYFEGLTYDHVNFIFSGASHSQDTVCPSTNSSYYK 60

Query: 61  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 120
           FG+SD WS+SYFDAQ  EV  HES + EHRRP   ST+ NEQS GN  WEENANPNMS  
Sbjct: 61  FGNSDLWSTSYFDAQLFEVQDHESAIYEHRRP--LSTVQNEQSLGNSVWEENANPNMSG- 120

Query: 121 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNV 180
            N+MECPRRH NY EYQTIWQDIVDPDNMTYE                            
Sbjct: 121 -NSMECPRRHSNYHEYQTIWQDIVDPDNMTYE---------------------------- 180

Query: 181 ETQEMLVQINLELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 240
                      EL+DLGETVGT +RGL QELIALLPVS Y+ GFF  KKSRNERCVICQM
Sbjct: 181 -----------ELVDLGETVGTQSRGLTQELIALLPVSSYRWGFFSRKKSRNERCVICQM 240

Query: 241 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 287
           EYKRGD+ ITLPCKHRYHT CGTKWLSI KACPICYTEVFG TSKR
Sbjct: 241 EYKRGDKIITLPCKHRYHTSCGTKWLSIKKACPICYTEVFGDTSKR 242

[Close]

BLAST of MS010205 vs. ExPASy TrEMBL
Match: A0A6J1K714 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita maxima OX=3661 GN=LOC111490765 PE=4 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.7e-102
Identity = 191/286 (66.78%), Postives = 215/286 (75.17%), Query Frame = 0


Query: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 60
           MSWN+++EV+CMNN YPYSTA SFMEYFEGLTY+HVNFIFS  SHS+ETV P+TNSSYYK
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTA-SFMEYFEGLTYDHVNFIFSAPSHSEETVCPATNSSYYK 60

Query: 61  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 120
           F H+DSWS+S FD QS EV GHES +DEHR  Q+FST+P+EQS GNR  EEN NP+M+S 
Sbjct: 61  FEHADSWSASCFDTQSFEVQGHESVIDEHRMQQEFSTVPDEQSLGNRVREENVNPDMAS- 120

Query: 121 TNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNV 180
            N+MECPRRH NY EYQTIWQDI+DPDNMTYE                            
Sbjct: 121 -NSMECPRRHSNYHEYQTIWQDIIDPDNMTYE---------------------------- 180

Query: 181 ETQEMLVQINLELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQM 240
                      EL+DLGETVG+ +RGL QELIALLPVSK+KC FF  KKSR ERCVICQM
Sbjct: 181 -----------ELVDLGETVGSQSRGLSQELIALLPVSKFKCWFFSRKKSRTERCVICQM 240

Query: 241 EYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSKR 287
            YK GDQRITLPCKH+YHT CG+KWLSINKACPICYTEV+G  SKR
Sbjct: 241 NYKHGDQRITLPCKHKYHTSCGSKWLSINKACPICYTEVYGDISKR 244

[Close]

BLAST of MS010205 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 156.0 bits (393), Expect = 4.6e-38
Identity = 92/270 (34.07%), Postives = 135/270 (50.00%), Query Frame = 0


Query: 16  YPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYKFGHSDSWSSSYFDAQ 75
           +PY+  GS+M+++ G     +N+  +   H Q+ +Y + N++ YKFG S S ++S++   
Sbjct: 18  FPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNAYKFGFSGSDNASFYG-- 77

Query: 76  SSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENAN-PNMSSNTNTMECPRRHPNYQ 135
           S ++  H S +   R   D+  + N           +    +   ++   EC     +  
Sbjct: 78  SYDMNDHLSRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDTDEHSEAEECIANEHDPD 137

Query: 136 EYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNVETQEMLVQINLELL 195
             Q  WQD +DPD MTYE                                       EL+
Sbjct: 138 SPQVSWQDDIDPDTMTYE---------------------------------------ELV 197

Query: 196 DLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQMEYKRGDQRITLPCK 255
           +LGE VGT +RGL QELI  LP  KYK G    +K   ERCVICQ++YK G++++ LPCK
Sbjct: 198 ELGEAVGTESRGLSQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCK 246

Query: 256 HRYHTCCGTKWLSINKACPICYTEVFGGTS 285
           H YH+ C +KWLSINK CP+C +EVFG  S
Sbjct: 258 HVYHSECISKWLSINKVCPVCNSEVFGEPS 246

[Close]

BLAST of MS010205 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 156.0 bits (393), Expect = 4.6e-38
Identity = 92/270 (34.07%), Postives = 135/270 (50.00%), Query Frame = 0


Query: 16  YPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYKFGHSDSWSSSYFDAQ 75
           +PY+  GS+M+++ G     +N+  +   H Q+ +Y + N++ YKFG S S ++S++   
Sbjct: 18  FPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNAYKFGFSGSDNASFYG-- 77

Query: 76  SSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENAN-PNMSSNTNTMECPRRHPNYQ 135
           S ++  H S +   R   D+  + N           +    +   ++   EC     +  
Sbjct: 78  SYDMNDHLSRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDTDEHSEAEECIANEHDPD 137

Query: 136 EYQTIWQDIVDPDNMTYEPMAKLVAIAATKLKLAVGLVFDAFLTNVETQEMLVQINLELL 195
             Q  WQD +DPD MTYE                                       EL+
Sbjct: 138 SPQVSWQDDIDPDTMTYE---------------------------------------ELV 197

Query: 196 DLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQMEYKRGDQRITLPCK 255
           +LGE VGT +RGL QELI  LP  KYK G    +K   ERCVICQ++YK G++++ LPCK
Sbjct: 198 ELGEAVGTESRGLSQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCK 246

Query: 256 HRYHTCCGTKWLSINKACPICYTEVFGGTS 285
           H YH+ C +KWLSINK CP+C +EVFG  S
Sbjct: 258 HVYHSECISKWLSINKVCPVCNSEVFGEPS 246

[Close]

BLAST of MS010205 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 97.1 bits (240), Expect = 2.5e-20
Identity = 46/93 (49.46%), Postives = 58/93 (62.37%), Query Frame = 0


Query: 192 ELLDLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQMEYKRGDQRITL 251
           ELL LG+ VGT +RGL  + IA LP  +YK G    +   NE CVIC+++Y+  +  I L
Sbjct: 247 ELLALGDIVGTESRGLSADTIASLPSKRYKEGD--NQNGTNESCVICRLDYEDDEDLILL 306

Query: 252 PCKHRYHTCCGTKWLSINKACPICYTEVFGGTS 285
           PCKH YH+ C   WL INK CP+C  EV   TS
Sbjct: 307 PCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

[Close]

BLAST of MS010205 vs. TAIR 10
Match: AT5G52140.1 (RING/U-box superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 3.8e-16
Identity = 43/97 (44.33%), Postives = 57/97 (58.76%), Query Frame = 0


Query: 192 ELLDLGETVGTHNRGLPQELIALLPVSKY----KCGFFLMKKSR----NERCVICQMEYK 251
           EL +LG++VG   +GL QE I+ L   KY    K  + L  K +    + +C IC MEY 
Sbjct: 183 ELTELGDSVGDVGKGLSQERISRLRTHKYGTKTKSWYCLHMKKKFVADDSQCSICLMEYA 242

Query: 252 RGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVF 281
           +GD+  TLPCKH YH  C ++WL  NK C IC  EV+
Sbjct: 243 KGDKITTLPCKHIYHKDCISQWLKQNKVCCICKAEVY 279

[Close]

BLAST of MS010205 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 80.1 bits (196), Expect = 3.2e-15
Identity = 40/89 (44.94%), Postives = 53/89 (59.55%), Query Frame = 0


Query: 192 ELLDLGETVGTHNRGL-PQELIALLPVSKYKCGFFLMKKSRNERCVICQMEYKRGDQRIT 251
           EL+ LG+ +G  NRGL P E+   L  S Y    F   K+  +RCV+CQME++  +  + 
Sbjct: 121 ELIALGDFIGVENRGLTPIEISTCLNASTY---VFSHNKNEIDRCVVCQMEFEERESLVV 180

Query: 252 L-PCKHRYHTCCGTKWLSINKACPICYTE 279
           L PC H YH+ C TKWL   K CPIC +E
Sbjct: 181 LRPCDHPYHSECITKWLETKKICPICCSE 206

The following BLAST results are available for this feature:

BLAST of MS010205 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148861.1	1.6e-138	86.36	E3 ubiquitin ligase BIG BROTHER-like isoform X2 [Momordica charantia]	[more]
XP_022148859.1	1.6e-138	86.36	E3 ubiquitin ligase BIG BROTHER-like isoform X1 [Momordica charantia] >XP_022148...	[more]
XP_038901510.1	1.2e-112	73.43	E3 ubiquitin-protein ligase BIG BROTHER-like [Benincasa hispida]	[more]
XP_004142225.1	1.8e-105	70.98	E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >XP_0116535...	[more]
XP_008449725.1	5.9e-104	69.93	PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X1 [Cucumis melo]	[more]

BLAST of MS010205 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L649	6.4e-37	34.07	E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE...	[more]
Q9LT17	3.5e-19	49.46	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P...	[more]
O49500	1.3e-10	38.37	E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV...	[more]
Q7XTV7	2.5e-09	38.10	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399...	[more]
Q9ZQF9	2.5e-09	39.56	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV...	[more]

BLAST of MS010205 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D445	7.9e-139	86.36	E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1D6M7	7.9e-139	86.36	E3 ubiquitin ligase BIG BROTHER-like isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A0A0L1Z5	8.8e-106	70.98	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G290860 P...	[more]
A0A1S3BNC2	2.8e-104	69.93	E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1K714	2.7e-102	66.78	E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita maxima OX=3661 GN=LOC111490765...	[more]

BLAST of MS010205 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63530.1	4.6e-38	34.07	RING/U-box superfamily protein	[more]
AT3G63530.2	4.6e-38	34.07	RING/U-box superfamily protein	[more]
AT3G19910.1	2.5e-20	49.46	RING/U-box superfamily protein	[more]
AT5G52140.1	3.8e-16	44.33	RING/U-box superfamily protein	[more]
AT3G47180.1	3.2e-15	44.94	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 235..275 e-value: 2.1E-4 score: 30.6
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 234..275 e-value: 3.1E-9 score: 37.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 235..275 score: 10.600894
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 198..280 e-value: 9.9E-15 score: 56.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 82..122
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 96..122
None	No IPR available	PANTHER	PTHR46400:SF5	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..157 coord: 192..284
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 229..280
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 192..284
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010205.1	MS010205.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity