MS009973 (gene) Bitter gourd (TR) v1

Overview
NameMS009973
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAquaporin TIP1-1
Locationscaffold943_1: 450452 .. 451307 (+)
RNA-Seq ExpressionMS009973
SyntenyMS009973
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGATCCGTAACATTGCCATCGGAAGGCCGGAGGAGGCGACCCACCCGGACGCCTTGAAGGCCGGTTTGGCCGAGTTTATTTCCACCTTGATTTTCGTATTCGCCGGCCAAGGTTCCGGCATAGCCTTCAGCAAGCTCACGGCCGGCGGCGCCGCCACTCCGGCCGGCCTCATCTCCGCCTCCATCGCCCACGCCTTCGCCCTCTTCGTCGCCGTCTCCGTCGGCGCCAACATCTCCGGTGGCCACGTCAACCCCGCCGTCACCTTCGGCGCCTTCGTCGGCGGCAACATCACCCTCCTACGTGGCCTCGTCTACTGGGTCGCCCAGCTCCTCGGATCCGTCGTCGCTTGCTTGCTCCTCGAGTTCGTTACCGGCATGGTATGAGAGGGAGTTAGTTAGTTATTTCCGTTAACAAGATCACGCATTTTGTATATATATATTATGTTAACGGTTTTTTGTTGGGTTTCGTTCCTGTGTTTCGCAGCCCACCGGAAGCTTCGCCCTTTCGGCTGGAGTTGGTGAAGTAAACGCCTTGGTTTTCGAGATCGTGATGACGTTCGGATTGGTGTACACGGTGTACGCGACCGCAGTGGATCCCAAGAAGGGCAGTTTGGGAACAATTGCACCCATCGCGATCGGTTTCATCGTGGGAGCTAATATTCTGGCCGGCGGAGCGTTCACCGGAGCGTCGATGAACCCGGCGGTGGCATTTGGGCCGTCGGTGGTGAGCTGGTCGTGGGACAGCCACTGGGTCTACTGGGCCGGGCCTCTGATCGGAGGTGGGCTTGCTGGGCTCATCTACGAGTTCATTTTCATCTCCCACTCCCACGAGCAGCTGCCCACCACCGATTAC

mRNA sequence

ATGCCGATCCGTAACATTGCCATCGGAAGGCCGGAGGAGGCGACCCACCCGGACGCCTTGAAGGCCGGTTTGGCCGAGTTTATTTCCACCTTGATTTTCGTATTCGCCGGCCAAGGTTCCGGCATAGCCTTCAGCAAGCTCACGGCCGGCGGCGCCGCCACTCCGGCCGGCCTCATCTCCGCCTCCATCGCCCACGCCTTCGCCCTCTTCGTCGCCGTCTCCGTCGGCGCCAACATCTCCGGTGGCCACGTCAACCCCGCCGTCACCTTCGGCGCCTTCGTCGGCGGCAACATCACCCTCCTACGTGGCCTCGTCTACTGGGTCGCCCAGCTCCTCGGATCCGTCGTCGCTTGCTTGCTCCTCGAGTTCGTTACCGGCATGCCCACCGGAAGCTTCGCCCTTTCGGCTGGAGTTGGTGAAGTAAACGCCTTGGTTTTCGAGATCGTGATGACGTTCGGATTGGTGTACACGGTGTACGCGACCGCAGTGGATCCCAAGAAGGGCAGTTTGGGAACAATTGCACCCATCGCGATCGGTTTCATCGTGGGAGCTAATATTCTGGCCGGCGGAGCGTTCACCGGAGCGTCGATGAACCCGGCGGTGGCATTTGGGCCGTCGGTGGTGAGCTGGTCGTGGGACAGCCACTGGGTCTACTGGGCCGGGCCTCTGATCGGAGGTGGGCTTGCTGGGCTCATCTACGAGTTCATTTTCATCTCCCACTCCCACGAGCAGCTGCCCACCACCGATTAC

Coding sequence (CDS)

ATGCCGATCCGTAACATTGCCATCGGAAGGCCGGAGGAGGCGACCCACCCGGACGCCTTGAAGGCCGGTTTGGCCGAGTTTATTTCCACCTTGATTTTCGTATTCGCCGGCCAAGGTTCCGGCATAGCCTTCAGCAAGCTCACGGCCGGCGGCGCCGCCACTCCGGCCGGCCTCATCTCCGCCTCCATCGCCCACGCCTTCGCCCTCTTCGTCGCCGTCTCCGTCGGCGCCAACATCTCCGGTGGCCACGTCAACCCCGCCGTCACCTTCGGCGCCTTCGTCGGCGGCAACATCACCCTCCTACGTGGCCTCGTCTACTGGGTCGCCCAGCTCCTCGGATCCGTCGTCGCTTGCTTGCTCCTCGAGTTCGTTACCGGCATGCCCACCGGAAGCTTCGCCCTTTCGGCTGGAGTTGGTGAAGTAAACGCCTTGGTTTTCGAGATCGTGATGACGTTCGGATTGGTGTACACGGTGTACGCGACCGCAGTGGATCCCAAGAAGGGCAGTTTGGGAACAATTGCACCCATCGCGATCGGTTTCATCGTGGGAGCTAATATTCTGGCCGGCGGAGCGTTCACCGGAGCGTCGATGAACCCGGCGGTGGCATTTGGGCCGTCGGTGGTGAGCTGGTCGTGGGACAGCCACTGGGTCTACTGGGCCGGGCCTCTGATCGGAGGTGGGCTTGCTGGGCTCATCTACGAGTTCATTTTCATCTCCCACTCCCACGAGCAGCTGCCCACCACCGATTAC

Protein sequence

MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLISASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLLLEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISHSHEQLPTTDY
Homology
BLAST of MS009973 vs. NCBI nr
Match: XP_004143284.1 (aquaporin TIP1-1 [Cucumis sativus] >KGN48203.1 hypothetical protein Csa_002806 [Cucumis sativus])

HSP 1 Score: 466.5 bits (1199), Expect = 1.5e-127
Identity = 237/250 (94.80%), Postives = 247/250 (98.80%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAF+KLT  GAATPAGLIS
Sbjct: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFNKLTNDGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRG++YW+AQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180
           L+FVTG+PTGSFALSAGVGE+NA VFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF
Sbjct: 121 LKFVTGLPTGSFALSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180

Query: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISH 240
           IVGANILAGGAFTGASMNPAVAFGPSVVSWSW+SHW+YWAGPLIGGGLAGLIYEFIFIS+
Sbjct: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWESHWIYWAGPLIGGGLAGLIYEFIFISN 240

Query: 241 SHEQLPTTDY 251
           SHEQLPTTDY
Sbjct: 241 SHEQLPTTDY 250

BLAST of MS009973 vs. NCBI nr
Match: NP_001284655.1 (aquaporin TIP1-1 [Cucumis melo] >AGW99230.1 aquaporin TIP1-1 [Cucumis melo])

HSP 1 Score: 466.5 bits (1199), Expect = 1.5e-127
Identity = 238/250 (95.20%), Postives = 246/250 (98.40%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLT  GAATPAGLIS
Sbjct: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTNDGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRG++YW+AQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180
           L+FVTG+PTGSF LSAGVGE+NA VFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF
Sbjct: 121 LKFVTGLPTGSFGLSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180

Query: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISH 240
           IVGANILAGGAFTGASMNPAVAFGPSVVSWSW+SHWVYWAGPLIGGGLAGLIYEFIFIS+
Sbjct: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWESHWVYWAGPLIGGGLAGLIYEFIFISN 240

Query: 241 SHEQLPTTDY 251
           SHEQLPTTDY
Sbjct: 241 SHEQLPTTDY 250

BLAST of MS009973 vs. NCBI nr
Match: XP_022978995.1 (aquaporin TIP1-1 [Cucurbita maxima] >XP_023544236.1 aquaporin TIP1-1 [Cucurbita pepo subsp. pepo] >KAG6604390.1 Aquaporin TIP1-1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034540.1 Aquaporin TIP1-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 466.1 bits (1198), Expect = 1.9e-127
Identity = 237/250 (94.80%), Postives = 247/250 (98.80%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIA+GRP+EAT PDALKAGLAEFISTLIFVFAGQGSGIAFSKLT GGAATPAGLIS
Sbjct: 1   MPIRNIAVGRPDEATSPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTDGGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRG++YW+AQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180
           L+FVTG+PTGSFALSAGVGE+NA VFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF
Sbjct: 121 LKFVTGLPTGSFALSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180

Query: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISH 240
           IVGANILAGGAFTGASMNPAVAFGPSVVSWSWD+HWVYWAGPLIGGGLAGLIYEFIFIS+
Sbjct: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDNHWVYWAGPLIGGGLAGLIYEFIFISN 240

Query: 241 SHEQLPTTDY 251
           SHEQLPTTDY
Sbjct: 241 SHEQLPTTDY 250

BLAST of MS009973 vs. NCBI nr
Match: XP_022925724.1 (aquaporin TIP1-1 [Cucurbita moschata])

HSP 1 Score: 464.5 bits (1194), Expect = 5.6e-127
Identity = 236/250 (94.40%), Postives = 247/250 (98.80%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIA+GRP+EAT PDALKAGLAEFISTLIFVFAGQGSGIAFSKLT GGAATPAGLIS
Sbjct: 1   MPIRNIAVGRPDEATSPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTDGGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRG++YW+AQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180
           L+FVTG+PTGSFALSAGVGE+NA VFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF
Sbjct: 121 LKFVTGLPTGSFALSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180

Query: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISH 240
           IVGANILAGGAFTGASMNPAVAFGPSVVSWSWD+HWVYWAGPLIGGGLAGLIYEFIFIS+
Sbjct: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDNHWVYWAGPLIGGGLAGLIYEFIFISN 240

Query: 241 SHEQLPTTDY 251
           SHEQLP+TDY
Sbjct: 241 SHEQLPSTDY 250

BLAST of MS009973 vs. NCBI nr
Match: XP_022972778.1 (aquaporin TIP1-1-like [Cucurbita maxima])

HSP 1 Score: 462.6 bits (1189), Expect = 2.1e-126
Identity = 235/250 (94.00%), Postives = 245/250 (98.00%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIAIGRPEEAT PDALKAGLAEFISTLIFVFAGQGSGIAF KLT GGAATPAGLIS
Sbjct: 1   MPIRNIAIGRPEEATRPDALKAGLAEFISTLIFVFAGQGSGIAFGKLTDGGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRG++YW+AQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180
           L+FVTG+PTGSFALSAGVGEVNA VFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF
Sbjct: 121 LKFVTGLPTGSFALSAGVGEVNAFVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180

Query: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISH 240
           IVGANILAGGAFTGASMNPAVAFGPSVVSWSWD+HWVYWAGPL+GGGLAGLIYEFIFIS+
Sbjct: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDNHWVYWAGPLVGGGLAGLIYEFIFISN 240

Query: 241 SHEQLPTTDY 251
           +HEQLP T+Y
Sbjct: 241 NHEQLPVTEY 250

BLAST of MS009973 vs. ExPASy Swiss-Prot
Match: P25818 (Aquaporin TIP1-1 OS=Arabidopsis thaliana OX=3702 GN=TIP1-1 PE=1 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 3.7e-113
Identity = 204/251 (81.27%), Postives = 230/251 (91.63%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIAIGRP+EAT PDALKA LAEFISTLIFV AG GSG+AF+KLT  GA TP+GL++
Sbjct: 1   MPIRNIAIGRPDEATRPDALKAALAEFISTLIFVVAGSGSGMAFNKLTENGATTPSGLVA 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           A++AHAF LFVAVSVGANISGGHVNPAVTFGAF+GGNITLLRG++YW+AQLLGSVVACL+
Sbjct: 61  AAVAHAFGLFVAVSVGANISGGHVNPAVTFGAFIGGNITLLRGILYWIAQLLGSVVACLI 120

Query: 121 LEFVT-GMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIG 180
           L+F T G+   +F LSAGVG +NA VFEIVMTFGLVYTVYATA+DPK GSLGTIAPIAIG
Sbjct: 121 LKFATGGLAVPAFGLSAGVGVLNAFVFEIVMTFGLVYTVYATAIDPKNGSLGTIAPIAIG 180

Query: 181 FIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFIS 240
           FIVGANILAGGAF+GASMNPAVAFGP+VVSW+W +HWVYWAGPL+GGG+AGLIYE  FI+
Sbjct: 181 FIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVYWAGPLVGGGIAGLIYEVFFIN 240

Query: 241 HSHEQLPTTDY 251
            +HEQLPTTDY
Sbjct: 241 TTHEQLPTTDY 251

BLAST of MS009973 vs. ExPASy Swiss-Prot
Match: Q9FY14 (Probable aquaporin TIP-type OS=Medicago truncatula OX=3880 GN=AQP1 PE=1 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 3.4e-111
Identity = 206/250 (82.40%), Postives = 224/250 (89.60%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIA+G P+EATHPD LKAGLAEFIST IFVFAG GSGIA++KLT  GAATPAGLIS
Sbjct: 1   MPIRNIAVGTPQEATHPDTLKAGLAEFISTFIFVFAGSGSGIAYNKLTNDGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRG+VY +AQLLGS+VA  L
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIVYIIAQLLGSIVASAL 120

Query: 121 LEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180
           L FVT     +F LS GVG   ALV EIVMTFGLVYTVYATAVDPKKG++G IAPIAIGF
Sbjct: 121 LVFVTASSVPAFGLSEGVGVGPALVLEIVMTFGLVYTVYATAVDPKKGNIGIIAPIAIGF 180

Query: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISH 240
           IVGANIL GGAFTGASMNPAV+FGP+VVSWSW +HWVYWAGPLIGGG+AGL+YE +FI+ 
Sbjct: 181 IVGANILVGGAFTGASMNPAVSFGPAVVSWSWSNHWVYWAGPLIGGGIAGLVYEVLFINS 240

Query: 241 SHEQLPTTDY 251
           +HEQLPTTDY
Sbjct: 241 THEQLPTTDY 250

BLAST of MS009973 vs. ExPASy Swiss-Prot
Match: P50156 (Probable aquaporin TIP1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=TIP1-1 PE=2 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 2.2e-110
Identity = 206/251 (82.07%), Postives = 224/251 (89.24%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIA+G  +E  HP ALKA LAEFISTLIFVFAGQGSG+AFSKLT GGA TPAGLI+
Sbjct: 1   MPIRNIAVGSHQEVYHPGALKAALAEFISTLIFVFAGQGSGMAFSKLTGGGATTPAGLIA 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           A++AHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITL RGL+YW+AQLLGS VAC L
Sbjct: 61  AAVAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLFRGLLYWIAQLLGSTVACFL 120

Query: 121 LEFVT-GMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIG 180
           L F T G+ TG+F L+ GV    ALV EIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIG
Sbjct: 121 LRFSTGGLATGTFGLT-GVSVWEALVLEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIG 180

Query: 181 FIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFIS 240
           FIVGANIL GGAF GASMNPAV+FGP++VSWSW+S WVYW GPLIGGGLAG+IYE +FIS
Sbjct: 181 FIVGANILVGGAFDGASMNPAVSFGPALVSWSWESQWVYWVGPLIGGGLAGVIYEVLFIS 240

Query: 241 HSHEQLPTTDY 251
           H+HEQLPTTDY
Sbjct: 241 HTHEQLPTTDY 250

BLAST of MS009973 vs. ExPASy Swiss-Prot
Match: P42067 (Probable aquaporin TIP-type OS=Medicago sativa OX=3879 GN=MCP1 PE=2 SV=2)

HSP 1 Score: 394.4 bits (1012), Expect = 9.4e-109
Identity = 204/250 (81.60%), Postives = 222/250 (88.80%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIA+G P+EATHPD LKAGLAEFIST IFVFAG GSGIA++KLT  GAATPAGLIS
Sbjct: 1   MPIRNIAVGTPQEATHPDTLKAGLAEFISTFIFVFAGSGSGIAYNKLTNDGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAV FGAFVGGNITLLRG+VY +AQLLGS+V   L
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAV-FGAFVGGNITLLRGIVYIIAQLLGSIVCSAL 120

Query: 121 LEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180
           L FVT     +F LS GVG   ALV EIVMTFGLVYTVYATAVDPKKG++G IAPIAIGF
Sbjct: 121 LVFVTASSVPAFGLSEGVGVGPALVLEIVMTFGLVYTVYATAVDPKKGNIGIIAPIAIGF 180

Query: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISH 240
           IVGANIL GGAFTGASMNPAV+FGP+VVSWSW +HWVYWAGPLIGGG+AGL+YE +FI+ 
Sbjct: 181 IVGANILVGGAFTGASMNPAVSFGPAVVSWSWSNHWVYWAGPLIGGGIAGLVYEVLFINS 240

Query: 241 SHEQLPTTDY 251
           +HEQLPTTDY
Sbjct: 241 THEQLPTTDY 249

BLAST of MS009973 vs. ExPASy Swiss-Prot
Match: Q41963 (Aquaporin TIP1-2 OS=Arabidopsis thaliana OX=3702 GN=TIP1-2 PE=1 SV=2)

HSP 1 Score: 391.7 bits (1005), Expect = 6.1e-108
Identity = 201/253 (79.45%), Postives = 225/253 (88.93%), Query Frame = 0

Query: 1   MPIRNIAIGR-PEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLI 60
           MP RNIAIG   EE  HP+AL+A LAEFISTLIFVFAG GSGIAF+K+T  GA TP+GL+
Sbjct: 1   MPTRNIAIGGVQEEVYHPNALRAALAEFISTLIFVFAGSGSGIAFNKITDNGATTPSGLV 60

Query: 61  SASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACL 120
           +A++AHAF LFVAVSVGANISGGHVNPAVTFG  +GGNITLLRG++YW+AQLLGSV AC 
Sbjct: 61  AAALAHAFGLFVAVSVGANISGGHVNPAVTFGVLLGGNITLLRGILYWIAQLLGSVAACF 120

Query: 121 LLEFVT-GMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAI 180
           LL F T G P  +F LSAGVG +NALVFEIVMTFGLVYTVYATAVDPK GSLGTIAPIAI
Sbjct: 121 LLSFATGGEPIPAFGLSAGVGSLNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAI 180

Query: 181 GFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFI 240
           GFIVGANILAGGAF+GASMNPAVAFGP+VVSW+W +HWVYWAGPLIGGGLAG+IY+F+FI
Sbjct: 181 GFIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVYWAGPLIGGGLAGIIYDFVFI 240

Query: 241 -SHSHEQLPTTDY 251
             ++HEQLPTTDY
Sbjct: 241 DENAHEQLPTTDY 253

BLAST of MS009973 vs. ExPASy TrEMBL
Match: U3R786 (Aquaporin TIP1-1 OS=Cucumis melo OX=3656 GN=LOC103500838 PE=2 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 7.2e-128
Identity = 238/250 (95.20%), Postives = 246/250 (98.40%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLT  GAATPAGLIS
Sbjct: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTNDGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRG++YW+AQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180
           L+FVTG+PTGSF LSAGVGE+NA VFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF
Sbjct: 121 LKFVTGLPTGSFGLSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180

Query: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISH 240
           IVGANILAGGAFTGASMNPAVAFGPSVVSWSW+SHWVYWAGPLIGGGLAGLIYEFIFIS+
Sbjct: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWESHWVYWAGPLIGGGLAGLIYEFIFISN 240

Query: 241 SHEQLPTTDY 251
           SHEQLPTTDY
Sbjct: 241 SHEQLPTTDY 250

BLAST of MS009973 vs. ExPASy TrEMBL
Match: A0A0A0KF67 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448110 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 7.2e-128
Identity = 237/250 (94.80%), Postives = 247/250 (98.80%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAF+KLT  GAATPAGLIS
Sbjct: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFNKLTNDGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRG++YW+AQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180
           L+FVTG+PTGSFALSAGVGE+NA VFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF
Sbjct: 121 LKFVTGLPTGSFALSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180

Query: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISH 240
           IVGANILAGGAFTGASMNPAVAFGPSVVSWSW+SHW+YWAGPLIGGGLAGLIYEFIFIS+
Sbjct: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWESHWIYWAGPLIGGGLAGLIYEFIFISN 240

Query: 241 SHEQLPTTDY 251
           SHEQLPTTDY
Sbjct: 241 SHEQLPTTDY 250

BLAST of MS009973 vs. ExPASy TrEMBL
Match: A0A6J1IRX0 (aquaporin TIP1-1 OS=Cucurbita maxima OX=3661 GN=LOC111478777 PE=3 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 9.4e-128
Identity = 237/250 (94.80%), Postives = 247/250 (98.80%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIA+GRP+EAT PDALKAGLAEFISTLIFVFAGQGSGIAFSKLT GGAATPAGLIS
Sbjct: 1   MPIRNIAVGRPDEATSPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTDGGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRG++YW+AQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180
           L+FVTG+PTGSFALSAGVGE+NA VFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF
Sbjct: 121 LKFVTGLPTGSFALSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180

Query: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISH 240
           IVGANILAGGAFTGASMNPAVAFGPSVVSWSWD+HWVYWAGPLIGGGLAGLIYEFIFIS+
Sbjct: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDNHWVYWAGPLIGGGLAGLIYEFIFISN 240

Query: 241 SHEQLPTTDY 251
           SHEQLPTTDY
Sbjct: 241 SHEQLPTTDY 250

BLAST of MS009973 vs. ExPASy TrEMBL
Match: A0A6J1ED08 (aquaporin TIP1-1 OS=Cucurbita moschata OX=3662 GN=LOC111433053 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 2.7e-127
Identity = 236/250 (94.40%), Postives = 247/250 (98.80%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIA+GRP+EAT PDALKAGLAEFISTLIFVFAGQGSGIAFSKLT GGAATPAGLIS
Sbjct: 1   MPIRNIAVGRPDEATSPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTDGGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRG++YW+AQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180
           L+FVTG+PTGSFALSAGVGE+NA VFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF
Sbjct: 121 LKFVTGLPTGSFALSAGVGELNAFVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180

Query: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISH 240
           IVGANILAGGAFTGASMNPAVAFGPSVVSWSWD+HWVYWAGPLIGGGLAGLIYEFIFIS+
Sbjct: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDNHWVYWAGPLIGGGLAGLIYEFIFISN 240

Query: 241 SHEQLPTTDY 251
           SHEQLP+TDY
Sbjct: 241 SHEQLPSTDY 250

BLAST of MS009973 vs. ExPASy TrEMBL
Match: A0A6J1IB31 (aquaporin TIP1-1-like OS=Cucurbita maxima OX=3661 GN=LOC111471283 PE=3 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.0e-126
Identity = 235/250 (94.00%), Postives = 245/250 (98.00%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIAIGRPEEAT PDALKAGLAEFISTLIFVFAGQGSGIAF KLT GGAATPAGLIS
Sbjct: 1   MPIRNIAIGRPEEATRPDALKAGLAEFISTLIFVFAGQGSGIAFGKLTDGGAATPAGLIS 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRG++YW+AQLLGSVVACLL
Sbjct: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIIYWIAQLLGSVVACLL 120

Query: 121 LEFVTGMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180
           L+FVTG+PTGSFALSAGVGEVNA VFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF
Sbjct: 121 LKFVTGLPTGSFALSAGVGEVNAFVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGF 180

Query: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISH 240
           IVGANILAGGAFTGASMNPAVAFGPSVVSWSWD+HWVYWAGPL+GGGLAGLIYEFIFIS+
Sbjct: 181 IVGANILAGGAFTGASMNPAVAFGPSVVSWSWDNHWVYWAGPLVGGGLAGLIYEFIFISN 240

Query: 241 SHEQLPTTDY 251
           +HEQLP T+Y
Sbjct: 241 NHEQLPVTEY 250

BLAST of MS009973 vs. TAIR 10
Match: AT2G36830.1 (gamma tonoplast intrinsic protein )

HSP 1 Score: 409.1 bits (1050), Expect = 2.6e-114
Identity = 204/251 (81.27%), Postives = 230/251 (91.63%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPIRNIAIGRP+EAT PDALKA LAEFISTLIFV AG GSG+AF+KLT  GA TP+GL++
Sbjct: 1   MPIRNIAIGRPDEATRPDALKAALAEFISTLIFVVAGSGSGMAFNKLTENGATTPSGLVA 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           A++AHAF LFVAVSVGANISGGHVNPAVTFGAF+GGNITLLRG++YW+AQLLGSVVACL+
Sbjct: 61  AAVAHAFGLFVAVSVGANISGGHVNPAVTFGAFIGGNITLLRGILYWIAQLLGSVVACLI 120

Query: 121 LEFVT-GMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIG 180
           L+F T G+   +F LSAGVG +NA VFEIVMTFGLVYTVYATA+DPK GSLGTIAPIAIG
Sbjct: 121 LKFATGGLAVPAFGLSAGVGVLNAFVFEIVMTFGLVYTVYATAIDPKNGSLGTIAPIAIG 180

Query: 181 FIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFIS 240
           FIVGANILAGGAF+GASMNPAVAFGP+VVSW+W +HWVYWAGPL+GGG+AGLIYE  FI+
Sbjct: 181 FIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVYWAGPLVGGGIAGLIYEVFFIN 240

Query: 241 HSHEQLPTTDY 251
            +HEQLPTTDY
Sbjct: 241 TTHEQLPTTDY 251

BLAST of MS009973 vs. TAIR 10
Match: AT3G26520.1 (tonoplast intrinsic protein 2 )

HSP 1 Score: 391.7 bits (1005), Expect = 4.3e-109
Identity = 201/253 (79.45%), Postives = 225/253 (88.93%), Query Frame = 0

Query: 1   MPIRNIAIGR-PEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLI 60
           MP RNIAIG   EE  HP+AL+A LAEFISTLIFVFAG GSGIAF+K+T  GA TP+GL+
Sbjct: 1   MPTRNIAIGGVQEEVYHPNALRAALAEFISTLIFVFAGSGSGIAFNKITDNGATTPSGLV 60

Query: 61  SASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACL 120
           +A++AHAF LFVAVSVGANISGGHVNPAVTFG  +GGNITLLRG++YW+AQLLGSV AC 
Sbjct: 61  AAALAHAFGLFVAVSVGANISGGHVNPAVTFGVLLGGNITLLRGILYWIAQLLGSVAACF 120

Query: 121 LLEFVT-GMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAI 180
           LL F T G P  +F LSAGVG +NALVFEIVMTFGLVYTVYATAVDPK GSLGTIAPIAI
Sbjct: 121 LLSFATGGEPIPAFGLSAGVGSLNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAI 180

Query: 181 GFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFI 240
           GFIVGANILAGGAF+GASMNPAVAFGP+VVSW+W +HWVYWAGPLIGGGLAG+IY+F+FI
Sbjct: 181 GFIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVYWAGPLIGGGLAGIIYDFVFI 240

Query: 241 -SHSHEQLPTTDY 251
             ++HEQLPTTDY
Sbjct: 241 DENAHEQLPTTDY 253

BLAST of MS009973 vs. TAIR 10
Match: AT4G01470.1 (tonoplast intrinsic protein 1;3 )

HSP 1 Score: 356.7 bits (914), Expect = 1.5e-98
Identity = 180/252 (71.43%), Postives = 212/252 (84.13%), Query Frame = 0

Query: 1   MPIRNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLIS 60
           MPI  IAIG P EA+ PDA++A  AEF S +IFVFAGQGSG+A+ KLT  G ATPAGL++
Sbjct: 1   MPINRIAIGTPGEASRPDAIRAAFAEFFSMVIFVFAGQGSGMAYGKLTGDGPATPAGLVA 60

Query: 61  ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLL 120
           AS++HAFALFVAVSVGAN+SGGHVNPAVTFGAF+GGNITLLR ++YW+AQLLG+VVACLL
Sbjct: 61  ASLSHAFALFVAVSVGANVSGGHVNPAVTFGAFIGGNITLLRAILYWIAQLLGAVVACLL 120

Query: 121 LEFVT-GMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIG 180
           L+  T GM T +F+LS GV   NA+VFEIVMTFGLVYTVYATAVDPKKG +G IAP+AIG
Sbjct: 121 LKVSTGGMETAAFSLSYGVTPWNAVVFEIVMTFGLVYTVYATAVDPKKGDIGIIAPLAIG 180

Query: 181 FIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFI- 240
            IVGANIL GGAF GASMNPAV+FGP+VVSW W +HWVYW GP IG  +A ++Y+ IFI 
Sbjct: 181 LIVGANILVGGAFDGASMNPAVSFGPAVVSWIWTNHWVYWVGPFIGAAIAAIVYDTIFIG 240

Query: 241 SHSHEQLPTTDY 251
           S+ HE LP+ D+
Sbjct: 241 SNGHEPLPSNDF 252

BLAST of MS009973 vs. TAIR 10
Match: AT1G73190.1 (Aquaporin-like superfamily protein )

HSP 1 Score: 292.4 bits (747), Expect = 3.6e-79
Identity = 151/262 (57.63%), Postives = 186/262 (70.99%), Query Frame = 0

Query: 4   RNIAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKL-----TAGGAATPAGL 63
           R    GR +EATHPD+++A LAEF+ST +FVFA +GS ++  KL        G  TP GL
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAAEGSILSLDKLYWEHAAHAGTNTPGGL 66

Query: 64  ISASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVAC 123
           I  ++AHAFALF AVS   N+SGGHVNPAVTFGA VGG +T +R + YW+AQLLG+++AC
Sbjct: 67  ILVALAHAFALFAAVSAAINVSGGHVNPAVTFGALVGGRVTAIRAIYYWIAQLLGAILAC 126

Query: 124 LLLEFVT-GMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIA 183
           LLL   T GM    F L++GVG VN LV EI++TFGLVY VY+T +DPK+GSLG IAP+A
Sbjct: 127 LLLRLTTNGMRPVGFRLASGVGAVNGLVLEIILTFGLVYVVYSTLIDPKRGSLGIIAPLA 186

Query: 184 IGFIVGANILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIF 243
           IG IVGANIL GG F+GASMNPA AFGP++V W W  HW+YW GP IG  LA LIYE++ 
Sbjct: 187 IGLIVGANILVGGPFSGASMNPARAFGPALVGWRWHDHWIYWVGPFIGSALAALIYEYMV 246

Query: 244 I--------SHS-HEQLPTTDY 251
           I        +H  H+ L   DY
Sbjct: 247 IPTEPPTHHAHGVHQPLAPEDY 268

BLAST of MS009973 vs. TAIR 10
Match: AT5G47450.1 (tonoplast intrinsic protein 2;3 )

HSP 1 Score: 291.2 bits (744), Expect = 7.9e-79
Identity = 155/245 (63.27%), Postives = 188/245 (76.73%), Query Frame = 0

Query: 6   IAIGRPEEATHPDALKAGLAEFISTLIFVFAGQGSGIAFSKLTAGGAATPAGLISASIAH 65
           I +G   ++    +LKA L+EFI+TL+FVFAG GS +AF+KLT+ GA  PAGL++ +IAH
Sbjct: 4   IEVGSVGDSFSVSSLKAYLSEFIATLLFVFAGVGSAVAFAKLTSDGALDPAGLVAIAIAH 63

Query: 66  AFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGLVYWVAQLLGSVVACLLLEFVT 125
           AFALFV VS+ ANISGGH+NPAVT G  +GGNITL+ G  YW+AQ LGS+VACLLL FVT
Sbjct: 64  AFALFVGVSIAANISGGHLNPAVTLGLAIGGNITLITGFFYWIAQCLGSIVACLLLVFVT 123

Query: 126 -GMPTGSFALSAGVGEVNALVFEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGFIVGA 185
            G    +  +SAG+G V  +V EIV+TF LVYTVYATA DPKKGSLGTIAPIAIGFIVGA
Sbjct: 124 NGKSVPTHGVSAGLGAVEGVVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFIVGA 183

Query: 186 NILAGGAFTGASMNPAVAFGPSVVSWSWDSHWVYWAGPLIGGGLAGLIYEFIFISHSHEQ 245
           NILA G F+G SMNPA +FGP+VVS      W+YW GPL+GG LAGLIY  +FI  S+E 
Sbjct: 184 NILAAGPFSGGSMNPARSFGPAVVSGDLSQIWIYWVGPLVGGALAGLIYGDVFIG-SYEA 243

Query: 246 LPTTD 250
           + T +
Sbjct: 244 VETRE 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004143284.11.5e-12794.80aquaporin TIP1-1 [Cucumis sativus] >KGN48203.1 hypothetical protein Csa_002806 [... [more]
NP_001284655.11.5e-12795.20aquaporin TIP1-1 [Cucumis melo] >AGW99230.1 aquaporin TIP1-1 [Cucumis melo][more]
XP_022978995.11.9e-12794.80aquaporin TIP1-1 [Cucurbita maxima] >XP_023544236.1 aquaporin TIP1-1 [Cucurbita ... [more]
XP_022925724.15.6e-12794.40aquaporin TIP1-1 [Cucurbita moschata][more]
XP_022972778.12.1e-12694.00aquaporin TIP1-1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P258183.7e-11381.27Aquaporin TIP1-1 OS=Arabidopsis thaliana OX=3702 GN=TIP1-1 PE=1 SV=1[more]
Q9FY143.4e-11182.40Probable aquaporin TIP-type OS=Medicago truncatula OX=3880 GN=AQP1 PE=1 SV=1[more]
P501562.2e-11082.07Probable aquaporin TIP1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=TIP1-1 PE=... [more]
P420679.4e-10981.60Probable aquaporin TIP-type OS=Medicago sativa OX=3879 GN=MCP1 PE=2 SV=2[more]
Q419636.1e-10879.45Aquaporin TIP1-2 OS=Arabidopsis thaliana OX=3702 GN=TIP1-2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
U3R7867.2e-12895.20Aquaporin TIP1-1 OS=Cucumis melo OX=3656 GN=LOC103500838 PE=2 SV=1[more]
A0A0A0KF677.2e-12894.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448110 PE=3 SV=1[more]
A0A6J1IRX09.4e-12894.80aquaporin TIP1-1 OS=Cucurbita maxima OX=3661 GN=LOC111478777 PE=3 SV=1[more]
A0A6J1ED082.7e-12794.40aquaporin TIP1-1 OS=Cucurbita moschata OX=3662 GN=LOC111433053 PE=3 SV=1[more]
A0A6J1IB311.0e-12694.00aquaporin TIP1-1-like OS=Cucurbita maxima OX=3661 GN=LOC111471283 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G36830.12.6e-11481.27gamma tonoplast intrinsic protein [more]
AT3G26520.14.3e-10979.45tonoplast intrinsic protein 2 [more]
AT4G01470.11.5e-9871.43tonoplast intrinsic protein 1;3 [more]
AT1G73190.13.6e-7957.63Aquaporin-like superfamily protein [more]
AT5G47450.17.9e-7963.27tonoplast intrinsic protein 2;3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 146..164
score: 42.65
coord: 216..236
score: 43.5
coord: 65..89
score: 50.93
coord: 102..121
score: 38.49
coord: 179..201
score: 45.93
coord: 21..40
score: 45.07
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 14..233
e-value: 4.5E-74
score: 249.0
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 21..236
e-value: 1.24384E-65
score: 201.713
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 4..250
e-value: 1.1E-75
score: 256.0
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 13..246
NoneNo IPR availablePANTHERPTHR45665:SF2AQUAPORIN TIP1-1coord: 1..250
IPR034294Aquaporin transporterPANTHERPTHR45665AQUAPORIN-8coord: 1..250
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 83..91

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009973.1MS009973.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0015267 channel activity