MS009867 (gene) Bitter gourd (TR) v1

BLAST of MS009867 vs. NCBI nr
Match: XP_022148288.1 (uncharacterized protein LOC111016977 [Momordica charantia])

HSP 1 Score: 835.9 bits (2158), Expect = 1.6e-238
Identity = 426/431 (98.84%), Postives = 428/431 (99.30%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP 60
           MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP
Sbjct: 1   MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP 60

Query: 61  SSFFAGGRKLNHRNSFGFQDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI 120
           SSFFAGGRKLNHRNSFGF+DGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI
Sbjct: 61  SSFFAGGRKLNHRNSFGFRDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI 120

Query: 121 GRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPRA 180
           GRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPRA
Sbjct: 121 GRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPRA 180

Query: 181 NHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDVKSQK 240
           NHLAAKVRLKEDAEASKTTDSLPISENVALPIA PECNASPNPKSILKRKDNHIDVKSQK
Sbjct: 181 NHLAAKVRLKEDAEASKTTDSLPISENVALPIAVPECNASPNPKSILKRKDNHIDVKSQK 240

Query: 241 RVRFNPECKISQDFRGSKDISMEGNSSSEDAYVCNEATFSSAGYRTQVPDYIQNPSRYTH 300
           RVRFNPECKISQDFRGSKDISMEGNSS EDAYVCNEATFSSAGYRTQVPDYIQNPSRYTH
Sbjct: 241 RVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNPSRYTH 300

Query: 301 YTFDSSYDVDDEFNKKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVMI 360
           YTFDSSYDVDDEFN+KAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVMI
Sbjct: 301 YTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVMI 360

Query: 361 ENSALGSQNDQNGAGKEVAQQRGIAIGIDNQTDEVCSMEEDEPDKLELRRNSSQKPGRQY 420
           ENSALGSQNDQNGAGKEV QQRGIAIGIDNQTDEVCSMEEDEPDKLELRRNSSQKPGRQY
Sbjct: 361 ENSALGSQNDQNGAGKEVVQQRGIAIGIDNQTDEVCSMEEDEPDKLELRRNSSQKPGRQY 420

Query: 421 RMRAKMESEEE 432
           RMRAKMESEEE
Sbjct: 421 RMRAKMESEEE 431

BLAST of MS009867 vs. NCBI nr
Match: XP_023531936.1 (uncharacterized protein LOC111794053 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627.5 bits (1617), Expect = 8.7e-176
Identity = 334/438 (76.26%), Postives = 369/438 (84.25%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSST----STAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEP 60
           MED+FKVRVDRIFGSLSSSS+    STAA NS LSSLW LTDDE+ER+EWIK K D PEP
Sbjct: 1   MEDNFKVRVDRIFGSLSSSSSSSSYSTAAFNSPLSSLWCLTDDEVERREWIKGKVDQPEP 60

Query: 61  EPEPSSFFAGGRKLNHRNSFGFQDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEI 120
           E EP+SF AG RK+N RNSF F+D FEDD+  DLDE+PESNGSSS FPKPDDYGDEEWEI
Sbjct: 61  ELEPNSFLAGERKVNGRNSFRFRDDFEDDV-GDLDEDPESNGSSSNFPKPDDYGDEEWEI 120

Query: 121 KSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTR 180
           KS IGRDCTLD+EEEED YDKVAVGKEK GDRLYMKDI DCG+EIDSCTELPTS+RNFTR
Sbjct: 121 KSLIGRDCTLDFEEEEDEYDKVAVGKEKVGDRLYMKDITDCGVEIDSCTELPTSIRNFTR 180

Query: 181 DPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDV 240
           DPRANHLAAKVRLKEDAEASKT DSL +SEN A+ IA  ECNAS NPKSILKRKDN +D 
Sbjct: 181 DPRANHLAAKVRLKEDAEASKTMDSLHVSENGAVAIAVSECNASHNPKSILKRKDNPLDA 240

Query: 241 KSQKRVRFNPECKISQDFRGSKDISMEGNSSSEDAYVCNEATFSSAGYRTQVPDYIQNPS 300
           KS KRVRF+PEC+IS++ +GS+DISME NSS   A V  EATF S G+R Q PDY++NPS
Sbjct: 241 KSHKRVRFDPECEISREPQGSEDISMEANSSIGAAEVTTEATFQSQGFRPQAPDYLRNPS 300

Query: 301 RYTHYTFDSSYDVDDEFNKKAYLDFLQLVRGSKTME-SHEDDASAGPPKYITFIPKKKAG 360
           RYTHYTFDSS +VD+E NK AY+DFLQLVRGSKT+E SH DD S GPPK ITFIPKKKAG
Sbjct: 301 RYTHYTFDSSNEVDEESNKNAYMDFLQLVRGSKTIEPSHLDDFSTGPPKSITFIPKKKAG 360

Query: 361 DTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRRNS 420
           DTVM+EN   G QN QNG GKE+  Q+G+ IGI   D QTD+VCSMEEDEP+KLE RRNS
Sbjct: 361 DTVMLENPTPGEQNHQNGVGKELVHQKGMPIGIASVDAQTDDVCSMEEDEPEKLETRRNS 420

Query: 421 SQKPGRQYRMRAKMESEE 431
           SQK  RQYRMRAKM+SEE
Sbjct: 421 SQKTARQYRMRAKMDSEE 437

BLAST of MS009867 vs. NCBI nr
Match: XP_022928536.1 (uncharacterized protein LOC111435316 [Cucurbita moschata])

HSP 1 Score: 626.7 bits (1615), Expect = 1.5e-175
Identity = 334/439 (76.08%), Postives = 370/439 (84.28%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSST-----STAALNSSLSSLWSLTDDEIERKEWIKEKEDPPE 60
           MED+FKVRVDRIFGSLSSSS+     STAA NS LSSLW LTDDE+ER+EWIK K D PE
Sbjct: 1   MEDNFKVRVDRIFGSLSSSSSSSSSYSTAAFNSPLSSLWCLTDDEVERREWIKGKVDQPE 60

Query: 61  PEPEPSSFFAGGRKLNHRNSFGFQDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWE 120
           PE EP+SF AG RK++ RNSF F+D FEDD+  DLDENPESNGSSS FPKPDDYGDEEWE
Sbjct: 61  PELEPTSFLAGERKVSGRNSFRFRDDFEDDV-GDLDENPESNGSSSNFPKPDDYGDEEWE 120

Query: 121 IKSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFT 180
           IKS IGRDCTLD+EEEED YDKVAVGKEK GDRLYMKDI DCG+EIDSCTELPTS++NFT
Sbjct: 121 IKSLIGRDCTLDFEEEEDEYDKVAVGKEKVGDRLYMKDITDCGVEIDSCTELPTSIQNFT 180

Query: 181 RDPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHID 240
           RDPRANHLAAKVRLKEDAEASKT DSL +SEN A+ IA  ECNAS NPKSILKRKDNH+D
Sbjct: 181 RDPRANHLAAKVRLKEDAEASKTMDSLHVSENGAVAIAVSECNASQNPKSILKRKDNHLD 240

Query: 241 VKSQKRVRFNPECKISQDFRGSKDISMEGNSSSEDAYVCNEATFSSAGYRTQVPDYIQNP 300
            KS KRVRF+PEC+IS++ +GS+DISME NSS   A V NEATF S G+R Q PDY++NP
Sbjct: 241 AKSHKRVRFDPECEISRESQGSEDISMEANSSIGAAEVTNEATFHSQGFRPQAPDYLRNP 300

Query: 301 SRYTHYTFDSSYDVDDEFNKKAYLDFLQLVRGSKTMES-HEDDASAGPPKYITFIPKKKA 360
           SRYTHYTFDSS +VD+E NK AY+DFLQLVRGSKT+E  H DD S GPPK ITFIPKKKA
Sbjct: 301 SRYTHYTFDSSNEVDEESNKNAYMDFLQLVRGSKTIEPLHLDDTSTGPPKSITFIPKKKA 360

Query: 361 GDTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRRN 420
           GDTVM+EN   G QN QNG GKE+  Q+G+ IGI   D QTD+VCSMEEDEP+KLE RRN
Sbjct: 361 GDTVMLENPTPGEQNHQNGVGKEL-HQKGMPIGIASVDAQTDDVCSMEEDEPEKLETRRN 420

Query: 421 SSQKPGRQYRMRAKMESEE 431
           SSQK  RQYRMRAKM+SEE
Sbjct: 421 SSQKTARQYRMRAKMDSEE 437

BLAST of MS009867 vs. NCBI nr
Match: XP_022989648.1 (uncharacterized protein LOC111486668 [Cucurbita maxima])

HSP 1 Score: 626.7 bits (1615), Expect = 1.5e-175
Identity = 332/438 (75.80%), Postives = 367/438 (83.79%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSST----STAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEP 60
           MED+FKVRVDRIFGSLSSSS+    STAA NS LSSLW LTDDE+ER+EWIK K D PEP
Sbjct: 1   MEDNFKVRVDRIFGSLSSSSSSSSYSTAAFNSPLSSLWCLTDDEVERREWIKGKVDQPEP 60

Query: 61  EPEPSSFFAGGRKLNHRNSFGFQDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEI 120
           E EP+SF AG RK+N RNSF F+D FEDD+  DLDENPESNGSSS FPKPDDYGDEEWEI
Sbjct: 61  ELEPTSFLAGERKVNGRNSFRFRDDFEDDV-GDLDENPESNGSSSNFPKPDDYGDEEWEI 120

Query: 121 KSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTR 180
           KS IGRDCTLD+EEEED YDKVAVGKEK GDRLYMKDI DCG+EIDSCTELPTS+RNFTR
Sbjct: 121 KSLIGRDCTLDFEEEEDEYDKVAVGKEKVGDRLYMKDITDCGVEIDSCTELPTSIRNFTR 180

Query: 181 DPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDV 240
           DPRANHLAAKVRLKEDAEASKT DSL +SEN A+ IA  ECN S NPKSILKRKDNH+D 
Sbjct: 181 DPRANHLAAKVRLKEDAEASKTMDSLHVSENDAVAIAVSECNVSQNPKSILKRKDNHLDA 240

Query: 241 KSQKRVRFNPECKISQDFRGSKDISMEGNSSSEDAYVCNEATFSSAGYRTQVPDYIQNPS 300
           KS KRVRF+PEC+IS++ +GS+DISME NSS   A V NEATF S G+  Q PDY++NPS
Sbjct: 241 KSHKRVRFDPECEISRESQGSEDISMEANSSIGAAEVTNEATFQSQGFCRQAPDYLRNPS 300

Query: 301 RYTHYTFDSSYDVDDEFNKKAYLDFLQLVRGSKTMES-HEDDASAGPPKYITFIPKKKAG 360
           RYTHYTFDSS +VD+E NK AY+DFLQLVRGSKT+E  H DD+S GPPK ITFIPKKKAG
Sbjct: 301 RYTHYTFDSSNEVDEESNKNAYMDFLQLVRGSKTIEPLHLDDSSTGPPKSITFIPKKKAG 360

Query: 361 DTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRRNS 420
           DTVM+EN   G QN QNG GKE+  Q+G+ I I   D QTD+VCSMEEDEP+KLE RRN 
Sbjct: 361 DTVMLENPTPGEQNHQNGVGKELVHQKGMPISIASVDAQTDDVCSMEEDEPEKLETRRNI 420

Query: 421 SQKPGRQYRMRAKMESEE 431
           SQK  RQYRMRAKM+SEE
Sbjct: 421 SQKTARQYRMRAKMDSEE 437

BLAST of MS009867 vs. NCBI nr
Match: KAG6588861.1 (hypothetical protein SDJN03_17426, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022621.1 hypothetical protein SDJN02_16355, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 624.8 bits (1610), Expect = 5.6e-175
Identity = 334/440 (75.91%), Postives = 369/440 (83.86%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSST------STAALNSSLSSLWSLTDDEIERKEWIKEKEDPP 60
           MED+FKVRVDRIFGSLSSSS+      STAA NS LSSLW LTDDE+ER+EWIK K D P
Sbjct: 1   MEDNFKVRVDRIFGSLSSSSSSSSSSYSTAAFNSPLSSLWCLTDDEVERREWIKGKVDQP 60

Query: 61  EPEPEPSSFFAGGRKLNHRNSFGFQDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEW 120
           EPE EP+SF AG RK++ RNSF F+D FEDD+  DLDENPESNGSSS FPKPDDYGDEEW
Sbjct: 61  EPELEPTSFLAGERKVSGRNSFRFRDDFEDDV-GDLDENPESNGSSSNFPKPDDYGDEEW 120

Query: 121 EIKSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNF 180
           EIKS IGRDCTLD+EEEED YDKVAVGKEK GDRLYMKDI DCG+EIDSCTELPTS+RNF
Sbjct: 121 EIKSLIGRDCTLDFEEEEDEYDKVAVGKEKVGDRLYMKDITDCGVEIDSCTELPTSIRNF 180

Query: 181 TRDPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHI 240
           TRDPRANHLAAKVRLKEDAEASKT DSL +SEN A+ IA  ECNAS NPKSILKRKDNH+
Sbjct: 181 TRDPRANHLAAKVRLKEDAEASKTMDSLHVSENGAVAIAVSECNASQNPKSILKRKDNHL 240

Query: 241 DVKSQKRVRFNPECKISQDFRGSKDISMEGNSSSEDAYVCNEATFSSAGYRTQVPDYIQN 300
           D KS KRVRF+PEC+IS++ +GS+DISME NSS   A V NEATF S G+  Q PDY++N
Sbjct: 241 DAKSHKRVRFDPECEISRESQGSEDISMEANSSIGAAEVTNEATFHSQGFCPQAPDYLRN 300

Query: 301 PSRYTHYTFDSSYDVDDEFNKKAYLDFLQLVRGSKTMES-HEDDASAGPPKYITFIPKKK 360
           PSRYTHYTFDSS +VD+E NK AY+DFLQLVRGSKT+E  H DD S GPPK ITFIPKKK
Sbjct: 301 PSRYTHYTFDSSNEVDEESNKNAYMDFLQLVRGSKTIEPLHLDDTSTGPPKSITFIPKKK 360

Query: 361 AGDTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRR 420
           AGDTVM+EN   G QN QNG GKE+  Q+G+ IGI   D QTD+VCSMEEDEP+KLE RR
Sbjct: 361 AGDTVMLENPTPGEQNHQNGVGKEL-HQKGMPIGIASVDAQTDDVCSMEEDEPEKLETRR 420

Query: 421 NSSQKPGRQYRMRAKMESEE 431
           NSSQK  RQYRMRAKM+SEE
Sbjct: 421 NSSQKTARQYRMRAKMDSEE 438

BLAST of MS009867 vs. ExPASy TrEMBL
Match: A0A6J1D3P7 (Protein TSSC4 OS=Momordica charantia OX=3673 GN=LOC111016977 PE=3 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 7.8e-239
Identity = 426/431 (98.84%), Postives = 428/431 (99.30%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP 60
           MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP
Sbjct: 1   MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP 60

Query: 61  SSFFAGGRKLNHRNSFGFQDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI 120
           SSFFAGGRKLNHRNSFGF+DGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI
Sbjct: 61  SSFFAGGRKLNHRNSFGFRDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI 120

Query: 121 GRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPRA 180
           GRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPRA
Sbjct: 121 GRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPRA 180

Query: 181 NHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDVKSQK 240
           NHLAAKVRLKEDAEASKTTDSLPISENVALPIA PECNASPNPKSILKRKDNHIDVKSQK
Sbjct: 181 NHLAAKVRLKEDAEASKTTDSLPISENVALPIAVPECNASPNPKSILKRKDNHIDVKSQK 240

Query: 241 RVRFNPECKISQDFRGSKDISMEGNSSSEDAYVCNEATFSSAGYRTQVPDYIQNPSRYTH 300
           RVRFNPECKISQDFRGSKDISMEGNSS EDAYVCNEATFSSAGYRTQVPDYIQNPSRYTH
Sbjct: 241 RVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNPSRYTH 300

Query: 301 YTFDSSYDVDDEFNKKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVMI 360
           YTFDSSYDVDDEFN+KAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVMI
Sbjct: 301 YTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVMI 360

Query: 361 ENSALGSQNDQNGAGKEVAQQRGIAIGIDNQTDEVCSMEEDEPDKLELRRNSSQKPGRQY 420
           ENSALGSQNDQNGAGKEV QQRGIAIGIDNQTDEVCSMEEDEPDKLELRRNSSQKPGRQY
Sbjct: 361 ENSALGSQNDQNGAGKEVVQQRGIAIGIDNQTDEVCSMEEDEPDKLELRRNSSQKPGRQY 420

Query: 421 RMRAKMESEEE 432
           RMRAKMESEEE
Sbjct: 421 RMRAKMESEEE 431

BLAST of MS009867 vs. ExPASy TrEMBL
Match: A0A6J1JQY5 (Protein TSSC4 OS=Cucurbita maxima OX=3661 GN=LOC111486668 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 7.1e-176
Identity = 332/438 (75.80%), Postives = 367/438 (83.79%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSST----STAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEP 60
           MED+FKVRVDRIFGSLSSSS+    STAA NS LSSLW LTDDE+ER+EWIK K D PEP
Sbjct: 1   MEDNFKVRVDRIFGSLSSSSSSSSYSTAAFNSPLSSLWCLTDDEVERREWIKGKVDQPEP 60

Query: 61  EPEPSSFFAGGRKLNHRNSFGFQDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEI 120
           E EP+SF AG RK+N RNSF F+D FEDD+  DLDENPESNGSSS FPKPDDYGDEEWEI
Sbjct: 61  ELEPTSFLAGERKVNGRNSFRFRDDFEDDV-GDLDENPESNGSSSNFPKPDDYGDEEWEI 120

Query: 121 KSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTR 180
           KS IGRDCTLD+EEEED YDKVAVGKEK GDRLYMKDI DCG+EIDSCTELPTS+RNFTR
Sbjct: 121 KSLIGRDCTLDFEEEEDEYDKVAVGKEKVGDRLYMKDITDCGVEIDSCTELPTSIRNFTR 180

Query: 181 DPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDV 240
           DPRANHLAAKVRLKEDAEASKT DSL +SEN A+ IA  ECN S NPKSILKRKDNH+D 
Sbjct: 181 DPRANHLAAKVRLKEDAEASKTMDSLHVSENDAVAIAVSECNVSQNPKSILKRKDNHLDA 240

Query: 241 KSQKRVRFNPECKISQDFRGSKDISMEGNSSSEDAYVCNEATFSSAGYRTQVPDYIQNPS 300
           KS KRVRF+PEC+IS++ +GS+DISME NSS   A V NEATF S G+  Q PDY++NPS
Sbjct: 241 KSHKRVRFDPECEISRESQGSEDISMEANSSIGAAEVTNEATFQSQGFCRQAPDYLRNPS 300

Query: 301 RYTHYTFDSSYDVDDEFNKKAYLDFLQLVRGSKTMES-HEDDASAGPPKYITFIPKKKAG 360
           RYTHYTFDSS +VD+E NK AY+DFLQLVRGSKT+E  H DD+S GPPK ITFIPKKKAG
Sbjct: 301 RYTHYTFDSSNEVDEESNKNAYMDFLQLVRGSKTIEPLHLDDSSTGPPKSITFIPKKKAG 360

Query: 361 DTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRRNS 420
           DTVM+EN   G QN QNG GKE+  Q+G+ I I   D QTD+VCSMEEDEP+KLE RRN 
Sbjct: 361 DTVMLENPTPGEQNHQNGVGKELVHQKGMPISIASVDAQTDDVCSMEEDEPEKLETRRNI 420

Query: 421 SQKPGRQYRMRAKMESEE 431
           SQK  RQYRMRAKM+SEE
Sbjct: 421 SQKTARQYRMRAKMDSEE 437

BLAST of MS009867 vs. ExPASy TrEMBL
Match: A0A6J1EKJ9 (Protein TSSC4 OS=Cucurbita moschata OX=3662 GN=LOC111435316 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 7.1e-176
Identity = 334/439 (76.08%), Postives = 370/439 (84.28%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSST-----STAALNSSLSSLWSLTDDEIERKEWIKEKEDPPE 60
           MED+FKVRVDRIFGSLSSSS+     STAA NS LSSLW LTDDE+ER+EWIK K D PE
Sbjct: 1   MEDNFKVRVDRIFGSLSSSSSSSSSYSTAAFNSPLSSLWCLTDDEVERREWIKGKVDQPE 60

Query: 61  PEPEPSSFFAGGRKLNHRNSFGFQDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWE 120
           PE EP+SF AG RK++ RNSF F+D FEDD+  DLDENPESNGSSS FPKPDDYGDEEWE
Sbjct: 61  PELEPTSFLAGERKVSGRNSFRFRDDFEDDV-GDLDENPESNGSSSNFPKPDDYGDEEWE 120

Query: 121 IKSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFT 180
           IKS IGRDCTLD+EEEED YDKVAVGKEK GDRLYMKDI DCG+EIDSCTELPTS++NFT
Sbjct: 121 IKSLIGRDCTLDFEEEEDEYDKVAVGKEKVGDRLYMKDITDCGVEIDSCTELPTSIQNFT 180

Query: 181 RDPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHID 240
           RDPRANHLAAKVRLKEDAEASKT DSL +SEN A+ IA  ECNAS NPKSILKRKDNH+D
Sbjct: 181 RDPRANHLAAKVRLKEDAEASKTMDSLHVSENGAVAIAVSECNASQNPKSILKRKDNHLD 240

Query: 241 VKSQKRVRFNPECKISQDFRGSKDISMEGNSSSEDAYVCNEATFSSAGYRTQVPDYIQNP 300
            KS KRVRF+PEC+IS++ +GS+DISME NSS   A V NEATF S G+R Q PDY++NP
Sbjct: 241 AKSHKRVRFDPECEISRESQGSEDISMEANSSIGAAEVTNEATFHSQGFRPQAPDYLRNP 300

Query: 301 SRYTHYTFDSSYDVDDEFNKKAYLDFLQLVRGSKTMES-HEDDASAGPPKYITFIPKKKA 360
           SRYTHYTFDSS +VD+E NK AY+DFLQLVRGSKT+E  H DD S GPPK ITFIPKKKA
Sbjct: 301 SRYTHYTFDSSNEVDEESNKNAYMDFLQLVRGSKTIEPLHLDDTSTGPPKSITFIPKKKA 360

Query: 361 GDTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRRN 420
           GDTVM+EN   G QN QNG GKE+  Q+G+ IGI   D QTD+VCSMEEDEP+KLE RRN
Sbjct: 361 GDTVMLENPTPGEQNHQNGVGKEL-HQKGMPIGIASVDAQTDDVCSMEEDEPEKLETRRN 420

Query: 421 SSQKPGRQYRMRAKMESEE 431
           SSQK  RQYRMRAKM+SEE
Sbjct: 421 SSQKTARQYRMRAKMDSEE 437

BLAST of MS009867 vs. ExPASy TrEMBL
Match: A0A6J1IZZ9 (Protein TSSC4 OS=Cucurbita maxima OX=3661 GN=LOC111481475 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 1.0e-169
Identity = 324/436 (74.31%), Postives = 366/436 (83.94%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTST-AALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPE 60
           M+DSFKVRVDRIFGSLSS+STS+ AA NSS+ SLW LTDDEIER+EWIKEKE+  EPE E
Sbjct: 1   MDDSFKVRVDRIFGSLSSTSTSSPAAFNSSVRSLWCLTDDEIERREWIKEKEEQLEPELE 60

Query: 61  PSSFFAGGRKLNHRNSFGFQDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSS 120
           P+SFF GG+K++ R+SFGFQD FEDD   DLDENP SNGSSSKFPKPDDYGDEEWEIKSS
Sbjct: 61  PTSFFDGGKKVSGRSSFGFQDDFEDDELDDLDENPGSNGSSSKFPKPDDYGDEEWEIKSS 120

Query: 121 IGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPR 180
           IGRDCTLDYEEEED YDKVAVGKEK GDRLYMKD  DCGIEI SCTELPTS+RNFTRDPR
Sbjct: 121 IGRDCTLDYEEEEDEYDKVAVGKEKGGDRLYMKDATDCGIEIGSCTELPTSVRNFTRDPR 180

Query: 181 ANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDVKSQ 240
           ANHLAAKVRLKEDAEASKT DSL +S N  + IAD ECNAS NPKSILKRKDN++D K  
Sbjct: 181 ANHLAAKVRLKEDAEASKTIDSLHVSVNSTVAIADSECNASQNPKSILKRKDNYLDAKLH 240

Query: 241 KRVRFNPECKISQDFRGSKDISMEGNSSSEDAYVCNEATFSSAGYRTQVPDYIQNPSRYT 300
           KRVRF+PECK SQD +G KD +M+ NS    A V NEA + S    TQVPDY++NPSRYT
Sbjct: 241 KRVRFDPECKASQDSQGFKDFAMQANSLPGVAEVGNEANYPSQA--TQVPDYLRNPSRYT 300

Query: 301 HYTFDSSYDVDDEFNKKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVM 360
           HYTFDSS +VD+E NKKAY+DFLQLVR SKTMES++DD S GPP+ ITFIPKKKAGDT+M
Sbjct: 301 HYTFDSSKEVDEESNKKAYMDFLQLVRRSKTMESNQDDDSTGPPRSITFIPKKKAGDTIM 360

Query: 361 IENSALGSQ-NDQNGAGKEVAQQRGIAIGID---NQTDEVCSMEEDEPDKLELRRNSSQK 420
           +E+S+L  Q + QNG GKE+  Q+G+ IGI     Q D+VCSMEEDEPD+LE+R+NS QK
Sbjct: 361 LESSSLEEQSHHQNGVGKELVHQKGMPIGIAAVYTQNDDVCSMEEDEPDRLEIRKNSLQK 420

Query: 421 PGRQYRMRAKMESEEE 432
            GRQYRMRA +ESEEE
Sbjct: 421 SGRQYRMRA-LESEEE 433

BLAST of MS009867 vs. ExPASy TrEMBL
Match: A0A6J1F221 (Protein TSSC4 OS=Cucurbita moschata OX=3662 GN=LOC111441676 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 1.2e-167
Identity = 320/435 (73.56%), Postives = 364/435 (83.68%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTST-AALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPE 60
           M+DSFKVRVDRIFGSLSS+STS+ AA +S+  SLW LTDDEIER+EWIKEKE+  EPE E
Sbjct: 1   MDDSFKVRVDRIFGSLSSTSTSSPAAFSSTARSLWCLTDDEIERREWIKEKEEQLEPELE 60

Query: 61  PSSFFAGGRKLNHRNSFGFQDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSS 120
           P+SF  GGRK+  R+SFGFQD FEDD   DLDENP SNGSSSKFPKPDDYGDEEWEIKSS
Sbjct: 61  PTSFCDGGRKVRGRSSFGFQDDFEDDELDDLDENPGSNGSSSKFPKPDDYGDEEWEIKSS 120

Query: 121 IGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPR 180
           IGRDCTLDYEEEED YDKVAVGKEK GDRLYMKD  DCGIEI SCT+LPTS++NFTRDPR
Sbjct: 121 IGRDCTLDYEEEEDEYDKVAVGKEKGGDRLYMKDATDCGIEIGSCTKLPTSVQNFTRDPR 180

Query: 181 ANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDVKSQ 240
           ANHLAAKVRL+EDAEASKT DSL +SEN  + IAD ECN S NPKSILKRKDN++D KS 
Sbjct: 181 ANHLAAKVRLEEDAEASKTIDSLHMSENSTVAIADSECNTSQNPKSILKRKDNYLDAKSH 240

Query: 241 KRVRFNPECKISQDFRGSKDISMEGNSSSEDAYVCNEATFSSAGYRTQVPDYIQNPSRYT 300
           KRVRF+PECK+SQ+ +G KD +ME NS    A V NEA F S    TQVPDY++NPSRYT
Sbjct: 241 KRVRFDPECKVSQESQGFKDFAMEANSLPGVAEVGNEANFPSQA--TQVPDYLRNPSRYT 300

Query: 301 HYTFDSSYDVDDEFNKKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVM 360
           HYTFDSS +VD+E NKKAY+DFLQ+VR SKTMESH+DDAS GPPK ITFIPKKKAGDT+M
Sbjct: 301 HYTFDSSNEVDEESNKKAYMDFLQMVRRSKTMESHQDDASTGPPKSITFIPKKKAGDTIM 360

Query: 361 IENSALGSQ-NDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRRNSSQK 420
           +E+S+L  + + QNG GKE+  Q+G+ IGI   + Q D+VCSMEEDE D+LE R+NS QK
Sbjct: 361 LESSSLEERSHHQNGVGKELVHQKGMPIGIAAVNTQNDDVCSMEEDESDRLESRKNSLQK 420

Query: 421 PGRQYRMRAKMESEE 431
            GRQYRMRA +ESEE
Sbjct: 421 SGRQYRMRA-LESEE 432

BLAST of MS009867 vs. TAIR 10
Match: AT5G13970.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G13310.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 230.3 bits (586), Expect = 2.9e-60
Identity = 181/462 (39.18%), Postives = 245/462 (53.03%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP 60
           M++SF+VR+D++FGSL+SSSTS+A     +SSLW L +DEI+  +   EKE         
Sbjct: 1   MDESFRVRIDKVFGSLASSSTSSA----PVSSLWCLAEDEIDGNQRSGEKE--------- 60

Query: 61  SSFFAGGRKLNHRNSFGFQDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI 120
                    LN  +  G Q+   DDL  D     E  G SS+  KP DY DEEWEIK+SI
Sbjct: 61  -----LSESLNSFSDNGKQEHESDDLSID-----EEKGRSSELQKPSDYDDEEWEIKNSI 120

Query: 121 GRDCTLDYEEEEDVYDKVAVGKEKSGDRLY--MKDI-ADCGIEIDSCTELPTSLRNFTRD 180
           G D TLD EEE D  DKVA+     G+++Y  MKD+  D   E D   ELP S     +D
Sbjct: 121 GMDSTLDMEEELDDNDKVAL-----GEKVYCCMKDVNDDYETEADEWVELPASFNEREKD 180

Query: 181 PRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASP---------------- 240
           PRAN +AAK+RLKEDAEA    +SL +SE +   ++    N  P                
Sbjct: 181 PRANLIAAKLRLKEDAEAVNKLNSLHVSEELQDNLSMSTENEKPFVVSEDNLLGAFKESH 240

Query: 241 -------NPKSILKRKDNHI-DVKSQKRVRFNPECKISQDFRGSKDISMEGNSSSEDAYV 300
                    K ILKR++N   D KS KRVRF+ + K      G  D  ME +S +ED   
Sbjct: 241 VGSSDENGLKPILKRRENQADDSKSPKRVRFSSDVKDRTLTEGDNDSVMEASSPNEDKV- 300

Query: 301 CNEATFSSAGYRTQVPDYIQNPSRYTHYTFDSSYDVDDEFNKKAYLDFLQLVRGSKTMES 360
                   A Y T +PDY++NPS+YT YTF+S  +VD+E N+KAY+DFL ++R       
Sbjct: 301 -------EAVYPTGIPDYMRNPSKYTRYTFESG-EVDEESNRKAYMDFLNMIRS------ 360

Query: 361 HEDDASAGP----PKYITFIPKKKAGDTVMIENSALGSQNDQNGAGKEVAQQRGIAIGID 420
            +D++   P    P+ + F+PK+K     M E+       D  G        R +AI +D
Sbjct: 361 -KDESLVDPLMELPRSVAFVPKRKP----MAESKVENIDKDCEG--------RRVAIAVD 403

Query: 421 NQTD-EVCSMEEDEPDKLELRRNSSQKPGRQYRMRAKMESEE 431
              D  + +MEEDEP   E  ++ +++PGRQYR RAK + EE
Sbjct: 421 TIEDCTISAMEEDEP---ETAQHVTKRPGRQYRARAKEDPEE 403

BLAST of MS009867 vs. TAIR 10
Match: AT5G13310.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: 7 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G13970.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 121.7 bits (304), Expect = 1.4e-27
Identity = 144/450 (32.00%), Postives = 205/450 (45.56%), Query Frame = 0

Query: 2   EDSFKVRVDRIFGSLS---------SSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKED 61
           ++ F+ RV +IFGSL          S+S+S  A      S+W+L+D E+E++EW ++  D
Sbjct: 8   DEDFQSRVAKIFGSLPFSRPSSSKFSTSSSAPASRQQSGSVWTLSDTEVEKREWKRDSYD 67

Query: 62  PPEPEPEPSSFFAGGRKLNHRNSFGFQDGFEDDLEADLDENPESNGSSSKFPKPDDYGDE 121
             E  P  SSF               Q   EDDL+ D+D   + +G              
Sbjct: 68  RDE-IPCASSF---------DELLKQQKPSEDDLK-DIDCGEDFDG-------------- 127

Query: 122 EWEIKSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLR 181
            W I++S+G D TLD E EED YDKVA+G+E  G+         CG              
Sbjct: 128 VWSIRASMGLDRTLDDEAEEDEYDKVALGEENDGE--------GCG-------------- 187

Query: 182 NFTRDPRANHLAAKVRLKED-AEASKTTDSLPISENVALPIADPECNASPNPKSILKRKD 241
              RDPRAN++AA++RLKED  EA+K   S         P A+    A P  K ILKRK+
Sbjct: 188 -RIRDPRANYVAARIRLKEDEIEANKFNTSASQPSESKEPHAEESSEAMPR-KPILKRKE 247

Query: 242 NHID--VKSQKRVRFN---------PECKISQDFRGSKDISMEGNSSSEDAYVCNEATFS 301
           N  D   ++ KRVRF+         PE   S     SK +S +G S +            
Sbjct: 248 NSSDSEARTSKRVRFDSVPEETLKKPEDTCSAS-ASSKIVSHQGKSGA------------ 307

Query: 302 SAGYRTQVPDYIQNPSRYTHYTFDSSYDVDDEFNKKAYLDFLQLVRGSKTMESHEDDASA 361
                 +VPDY+ NPS YT Y+FD S ++D E     Y+D    V G K  ES       
Sbjct: 308 ------RVPDYLLNPSSYTRYSFDPSCELDVESPTGEYMDTPNAVEGLKNPESESF---- 367

Query: 362 GPPKYITFIPKKKAGDTVMIENSALGSQNDQNGAGKEVAQQRGIAIGIDNQTDEVCSMEE 421
             PK ++FIP+ K  D  + E+S+  ++     AG E+A++   +   D  T+    + E
Sbjct: 368 --PK-VSFIPQNKTKD--VSEDSSNCNETKPIVAG-ELAEEERPSATEDGDTE----IRE 369

Query: 422 DEPDKLELRRNSSQKPGRQYRMRAKMESEE 431
            E         S Q  GRQYR +  ++  +
Sbjct: 428 SE------SCTSFQSKGRQYRAKLSLDETD 369