MS009666 (gene) Bitter gourd (TR) v1

Overview
NameMS009666
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionKelch repeat-containing F-box family protein
Locationscaffold813: 3097817 .. 3099975 (-)
RNA-Seq ExpressionMS009666
SyntenyMS009666
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCACCGATCACTTCCCTAGCTCCGGACCACTTATTCACAGTTCTCCTCCGACTACCTGTAGATTCAATTCTCTCCTTCTCCATTACTTGTAGGAGATTTAGGGCTCTCACTTCCTCTGATACTCTCTGGGAAGCTATTTGCAGAAGGGAATGGGGATCCTCTTCAGTTGAGGCCCTCAAGTCCTATCATCTCTCTCAGCTGCCATGGATGAGGGTCTACAAGCAAGTTTCCCAGCTTGACTCTGTTTATTGCCATAGATTATCCGACCCAGATGCCGAATCATTACATCCCAGTCCTAGAGCTTCTCACTCCCTTAACTTCGTATCAGATTGCTTGGTACTGTTTGGTGGAGGATGCGAAGGAGGTTCGTGTTCTTTTTACTATACGAATCTCCTTTCTCTTCATGTTCTGCATCTTTTATCTTATTCATTGTGATTGTGCGTTGCCCTTGTTTTCCGTTATTCCGATTTCTTCGATTACTATTGCTTTCGCTTAGATTACAAGTGCGGATTGAACATTCATGTGTGCTGATCCCCATTCTTAATCTTGTTAACTTTAAACTCGAGTTAATTGGGGCGACTGCCTTTCGGAAGAGTTAGTTAATTAAACCATTTAATTTATAATTGTTATACCAAGGGAGGCCTCTTTAGTACCCACCCATGGGATGATGGGTTATTATTAAATCATGATGCTTTCTATATTACCAGCTCTATCTTTTTGCTACCTTTATTTGTTTAAGAGAGCAGATGGAGCTTCTGTATCTGCACCATCAGTTGTGTGTGTGGTATTTTGTTAGACGAGGAGATTATTCAGAGAAAATAGAAGGTTTTGTTCTTCGTTCATCATTTGTAGTTGTTGGCCACTTTATTATTGTTTCTTACAAATGGAACATATATAGCTACTCAACTTGCATCAACCGTGGGCTTATTCTTTATATTCTCAAAATATTTAAAACGCCAGCATATCTATAGACTATAGCTATTCTAGATTTATTGTTTTCTGCAAAACAGAATAAGAAAATTCTGGCCTCCGTAGTGGGGGAATGAATGAAATTGTCAGATTCTGAGACAGATGATAAATAAAGTAGACTTCTAACATGCACCTTCAGGCGAATAGCTTCACCATTAAAGGCCGACCTTCTTTGTCCTTATTATGTACATCTAAGCTCTATTTTGTACTTGTTTGTTGATTTAGGACGTCATCTTGATGACACGTGGGTGGCGTACATCGGTAATGATTGCCACAGGATGCTGAAGTGGCAGAAGATGAACTCAGGTATCCCAAGTGGGAGATTCGGACATACATGCACTGTTGTTGGTGATTTTCTGATTCTCTTTGGAGGAATCAAGGACAATGGAATTCGTCAGAATGATACATGGGTGGGAAGGATTGAAAGGAGTGAGATTCATGGTATTGGGTTCTCGTGGAGACGACTTGACGTTGGGGTGATAGCGCCACCACCTCGAGGAGCTCATGCTTCATGCTGCATAGATGAGAAGAAGATGGTTGTCCATGGAGGAATTGGGCTGGGTGGCATTAGATTGTTAGACACCTGGGTTTTAGAGCTGTCTGAGGATCTTAAATTTGGAAGCTGGCATGAGATTGTGGCTCATCCGTCTCCTCCAGCTCGATCAGGACATACACTTACTTCCATTGGGGGAACGAAAGTAGTTCTGTTTGGCGGTAGAGGAGTAGGGTATGATGTGCTGAATGATGTTTGGCTCTTGAATATATTTGATGGTCAGGTTAGATGGGTGCAAATTGTGTATGAGTTACAAAATATAAGTGAAGGAGTATCTCTCCCGCGAGTTGGACACTCCGCCAACCTCATCCTGGGAGGCCGCCTTCTAATTTATGGAGGGGAAGATACAGAGAGGCACCACAAAGACGACTTTTGGGCGTTGGATGTCGGTGCAATGAAATCAATCGAAGAGCAGCAGCTTACCACATCAAACTCCATGGGATTATCAGCGAAGATGTGGAAGAGATTAAAGGCAAATGGTTACACCCCCAGCTCTAGATCATTTCATCGCGCCTGTGCAGATCGTTCTGGTCGTTACTTATATGTCCTCGGGGGGATGGTGGATGGCCTAGTTCTGCCTGCTGAATCATCAGGACTCAGATTTGATGGAGATCTGTTTCTCGTGGAGTTGGTTATT

mRNA sequence

TCACCGATCACTTCCCTAGCTCCGGACCACTTATTCACAGTTCTCCTCCGACTACCTGTAGATTCAATTCTCTCCTTCTCCATTACTTGTAGGAGATTTAGGGCTCTCACTTCCTCTGATACTCTCTGGGAAGCTATTTGCAGAAGGGAATGGGGATCCTCTTCAGTTGAGGCCCTCAAGTCCTATCATCTCTCTCAGCTGCCATGGATGAGGGTCTACAAGCAAGTTTCCCAGCTTGACTCTGTTTATTGCCATAGATTATCCGACCCAGATGCCGAATCATTACATCCCAGTCCTAGAGCTTCTCACTCCCTTAACTTCGTATCAGATTGCTTGGTACTGTTTGGTGGAGGATGCGAAGGAGGACGTCATCTTGATGACACGTGGGTGGCGTACATCGGTAATGATTGCCACAGGATGCTGAAGTGGCAGAAGATGAACTCAGGTATCCCAAGTGGGAGATTCGGACATACATGCACTGTTGTTGGTGATTTTCTGATTCTCTTTGGAGGAATCAAGGACAATGGAATTCGTCAGAATGATACATGGGTGGGAAGGATTGAAAGGAGTGAGATTCATGGTATTGGGTTCTCGTGGAGACGACTTGACGTTGGGGTGATAGCGCCACCACCTCGAGGAGCTCATGCTTCATGCTGCATAGATGAGAAGAAGATGGTTGTCCATGGAGGAATTGGGCTGGGTGGCATTAGATTGTTAGACACCTGGGTTTTAGAGCTGTCTGAGGATCTTAAATTTGGAAGCTGGCATGAGATTGTGGCTCATCCGTCTCCTCCAGCTCGATCAGGACATACACTTACTTCCATTGGGGGAACGAAAGTAGTTCTGTTTGGCGGTAGAGGAGTAGGGTATGATGTGCTGAATGATGTTTGGCTCTTGAATATATTTGATGGTCAGGTTAGATGGGTGCAAATTGTGTATGAGTTACAAAATATAAGTGAAGGAGTATCTCTCCCGCGAGTTGGACACTCCGCCAACCTCATCCTGGGAGGCCGCCTTCTAATTTATGGAGGGGAAGATACAGAGAGGCACCACAAAGACGACTTTTGGGCGTTGGATGTCGGTGCAATGAAATCAATCGAAGAGCAGCAGCTTACCACATCAAACTCCATGGGATTATCAGCGAAGATGTGGAAGAGATTAAAGGCAAATGGTTACACCCCCAGCTCTAGATCATTTCATCGCGCCTGTGCAGATCGTTCTGGTCGTTACTTATATGTCCTCGGGGGGATGGTGGATGGCCTAGTTCTGCCTGCTGAATCATCAGGACTCAGATTTGATGGAGATCTGTTTCTCGTGGAGTTGGTTATT

Coding sequence (CDS)

TCACCGATCACTTCCCTAGCTCCGGACCACTTATTCACAGTTCTCCTCCGACTACCTGTAGATTCAATTCTCTCCTTCTCCATTACTTGTAGGAGATTTAGGGCTCTCACTTCCTCTGATACTCTCTGGGAAGCTATTTGCAGAAGGGAATGGGGATCCTCTTCAGTTGAGGCCCTCAAGTCCTATCATCTCTCTCAGCTGCCATGGATGAGGGTCTACAAGCAAGTTTCCCAGCTTGACTCTGTTTATTGCCATAGATTATCCGACCCAGATGCCGAATCATTACATCCCAGTCCTAGAGCTTCTCACTCCCTTAACTTCGTATCAGATTGCTTGGTACTGTTTGGTGGAGGATGCGAAGGAGGACGTCATCTTGATGACACGTGGGTGGCGTACATCGGTAATGATTGCCACAGGATGCTGAAGTGGCAGAAGATGAACTCAGGTATCCCAAGTGGGAGATTCGGACATACATGCACTGTTGTTGGTGATTTTCTGATTCTCTTTGGAGGAATCAAGGACAATGGAATTCGTCAGAATGATACATGGGTGGGAAGGATTGAAAGGAGTGAGATTCATGGTATTGGGTTCTCGTGGAGACGACTTGACGTTGGGGTGATAGCGCCACCACCTCGAGGAGCTCATGCTTCATGCTGCATAGATGAGAAGAAGATGGTTGTCCATGGAGGAATTGGGCTGGGTGGCATTAGATTGTTAGACACCTGGGTTTTAGAGCTGTCTGAGGATCTTAAATTTGGAAGCTGGCATGAGATTGTGGCTCATCCGTCTCCTCCAGCTCGATCAGGACATACACTTACTTCCATTGGGGGAACGAAAGTAGTTCTGTTTGGCGGTAGAGGAGTAGGGTATGATGTGCTGAATGATGTTTGGCTCTTGAATATATTTGATGGTCAGGTTAGATGGGTGCAAATTGTGTATGAGTTACAAAATATAAGTGAAGGAGTATCTCTCCCGCGAGTTGGACACTCCGCCAACCTCATCCTGGGAGGCCGCCTTCTAATTTATGGAGGGGAAGATACAGAGAGGCACCACAAAGACGACTTTTGGGCGTTGGATGTCGGTGCAATGAAATCAATCGAAGAGCAGCAGCTTACCACATCAAACTCCATGGGATTATCAGCGAAGATGTGGAAGAGATTAAAGGCAAATGGTTACACCCCCAGCTCTAGATCATTTCATCGCGCCTGTGCAGATCGTTCTGGTCGTTACTTATATGTCCTCGGGGGGATGGTGGATGGCCTAGTTCTGCCTGCTGAATCATCAGGACTCAGATTTGATGGAGATCTGTTTCTCGTGGAGTTGGTTATT

Protein sequence

SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALKSYHLSQLPWMRVYKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCEGGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIRQNDTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLDTWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWLLNIFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALDVGAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMVDGLVLPAESSGLRFDGDLFLVELVI
Homology
BLAST of MS009666 vs. NCBI nr
Match: XP_022149407.1 (F-box/kelch-repeat protein At1g51550 [Momordica charantia])

HSP 1 Score: 925.6 bits (2391), Expect = 1.6e-265
Identity = 442/443 (99.77%), Postives = 442/443 (99.77%), Query Frame = 0

Query: 1   SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 60
           SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK
Sbjct: 18  SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 77

Query: 61  SYHLSQLPWMRVYKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCE 120
           SYHLSQLPWMRVYKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCE
Sbjct: 78  SYHLSQLPWMRVYKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCE 137

Query: 121 GGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIRQN 180
           GGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIRQN
Sbjct: 138 GGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIRQN 197

Query: 181 DTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLD 240
           DTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLD
Sbjct: 198 DTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLD 257

Query: 241 TWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWLLN 300
           TWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWLLN
Sbjct: 258 TWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWLLN 317

Query: 301 IFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALDV 360
           IFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALDV
Sbjct: 318 IFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALDV 377

Query: 361 GAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMVDG 420
            AMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMVDG
Sbjct: 378 SAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMVDG 437

Query: 421 LVLPAESSGLRFDGDLFLVELVI 444
           LVLPAESSGLRFDGDLFLVELVI
Sbjct: 438 LVLPAESSGLRFDGDLFLVELVI 460

BLAST of MS009666 vs. NCBI nr
Match: KAG7013930.1 (F-box/kelch-repeat protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 793.5 bits (2048), Expect = 9.4e-226
Identity = 374/445 (84.04%), Postives = 406/445 (91.24%), Query Frame = 0

Query: 1   SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 60
           SPITSLAPDHLF +LL LPVDSILSFS+TC RFRALTSSD LWEAICRREWGS+SVEAL 
Sbjct: 16  SPITSLAPDHLFAILLLLPVDSILSFSVTCSRFRALTSSDALWEAICRREWGSASVEALN 75

Query: 61  SYHL-SQLPWMRVYKQVSQLDSVYCHRLSDPDAESLH-PSPRASHSLNFVSDCLVLFGGG 120
           SYHL S LPWMRV+KQ+S+L+SV CHRLSDPDAES   P PRASHSLNFVSDCLVLFGGG
Sbjct: 76  SYHLSSHLPWMRVFKQLSRLESVRCHRLSDPDAESSSLPGPRASHSLNFVSDCLVLFGGG 135

Query: 121 CEGGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIR 180
           CE GRHLDDTW+AYIGNDCHRML W+KMNSG PSGRFGHTC VVGDFLILFGGI DNGIR
Sbjct: 136 CESGRHLDDTWLAYIGNDCHRMLNWRKMNSGTPSGRFGHTCNVVGDFLILFGGINDNGIR 195

Query: 181 QNDTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRL 240
           QNDTW GR+ER  IHG  FSW  LDVG +APPPRGAHASCCID+KKMV+HGGIGLGGIRL
Sbjct: 196 QNDTWAGRVERKGIHGFEFSWIPLDVGELAPPPRGAHASCCIDDKKMVIHGGIGLGGIRL 255

Query: 241 LDTWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWL 300
            DTWVLELSE+LKFG+WHEIV+HPSPPARSGHTLTSIGG KVVLFGGRGVGYDVLND+WL
Sbjct: 256 FDTWVLELSENLKFGTWHEIVSHPSPPARSGHTLTSIGGMKVVLFGGRGVGYDVLNDIWL 315

Query: 301 LNIFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWAL 360
           L+IF+G  RWVQI+Y+LQN++EGVSLPRVGHSANLILGGRLLIYGGEDTERH KDDFWAL
Sbjct: 316 LSIFEGHGRWVQILYDLQNVTEGVSLPRVGHSANLILGGRLLIYGGEDTERHRKDDFWAL 375

Query: 361 DVGAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMV 420
           DV A+K+I + QLTT NS+GL+AKMWKRLK+NG TP+SRSFHRAC DRSGR LY+LGGMV
Sbjct: 376 DVSALKTI-QVQLTTGNSIGLAAKMWKRLKSNGCTPNSRSFHRACTDRSGRCLYILGGMV 435

Query: 421 DGLVLPAESSGLRFDGDLFLVELVI 444
           DG+V  AESSGLRFDGDLFLVELVI
Sbjct: 436 DGVVQAAESSGLRFDGDLFLVELVI 459

BLAST of MS009666 vs. NCBI nr
Match: XP_022992252.1 (F-box/kelch-repeat protein At1g51550 [Cucurbita maxima] >XP_022992253.1 F-box/kelch-repeat protein At1g51550 [Cucurbita maxima])

HSP 1 Score: 792.0 bits (2044), Expect = 2.7e-225
Identity = 373/445 (83.82%), Postives = 405/445 (91.01%), Query Frame = 0

Query: 1   SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 60
           SPITSLAPDHLF +LL LPVDSILSFS+TC RFRALTSSD LWEAICRREWGS+SVEAL 
Sbjct: 16  SPITSLAPDHLFAILLLLPVDSILSFSVTCSRFRALTSSDALWEAICRREWGSASVEALN 75

Query: 61  SYHL-SQLPWMRVYKQVSQLDSVYCHRLSDPDAESLH-PSPRASHSLNFVSDCLVLFGGG 120
           SYHL S LPWMRV+KQ+S+L+SV CHRLSDPDAES   P PRASHSLNFVSDCLVLFGGG
Sbjct: 76  SYHLSSHLPWMRVFKQLSRLESVRCHRLSDPDAESSSLPGPRASHSLNFVSDCLVLFGGG 135

Query: 121 CEGGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIR 180
           CE GRHLDDTW+AYIGNDCHRML W+KMNSG PSGRFGHTC VVGDFLILFGGI DNGIR
Sbjct: 136 CESGRHLDDTWLAYIGNDCHRMLNWRKMNSGTPSGRFGHTCNVVGDFLILFGGINDNGIR 195

Query: 181 QNDTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRL 240
           QNDTW GR+ER  IHG  FSW  LDVG +APPPRGAHASCCID+KKMV+HGGIGLGGIRL
Sbjct: 196 QNDTWAGRVERKGIHGYEFSWIPLDVGELAPPPRGAHASCCIDDKKMVIHGGIGLGGIRL 255

Query: 241 LDTWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWL 300
            DTWVLELSE+LKFG+WHEIV+HPSPPARSGHTL SIGG KVVLFGGRGVGYDV+ND+WL
Sbjct: 256 FDTWVLELSENLKFGTWHEIVSHPSPPARSGHTLNSIGGMKVVLFGGRGVGYDVMNDIWL 315

Query: 301 LNIFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWAL 360
           L++F+G  RWVQI+Y+LQN++EGVSLPRVGHSANLILGGRLLIYGGEDTERH KDDFWAL
Sbjct: 316 LSLFEGHGRWVQILYDLQNVTEGVSLPRVGHSANLILGGRLLIYGGEDTERHRKDDFWAL 375

Query: 361 DVGAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMV 420
           DV A+K+I E QLTT NS+GL+AKMWKRLKANG TP+SRSFHRAC DRSGR LY+LGGMV
Sbjct: 376 DVSALKTI-EVQLTTGNSIGLAAKMWKRLKANGCTPNSRSFHRACTDRSGRCLYILGGMV 435

Query: 421 DGLVLPAESSGLRFDGDLFLVELVI 444
           DG+V  AESSGLRFDGDLFLVELVI
Sbjct: 436 DGVVQAAESSGLRFDGDLFLVELVI 459

BLAST of MS009666 vs. NCBI nr
Match: XP_022953698.1 (F-box/kelch-repeat protein At1g51550 [Cucurbita moschata])

HSP 1 Score: 790.4 bits (2040), Expect = 7.9e-225
Identity = 374/445 (84.04%), Postives = 405/445 (91.01%), Query Frame = 0

Query: 1   SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 60
           SPITSLAPDHLF +LL LPVDSILSFS+TC RFRALTSSD LWEAICRREWGS+SVEAL 
Sbjct: 16  SPITSLAPDHLFAILLLLPVDSILSFSVTCSRFRALTSSDALWEAICRREWGSASVEALN 75

Query: 61  SYHL-SQLPWMRVYKQVSQLDSVYCHRLSDPDAESLH-PSPRASHSLNFVSDCLVLFGGG 120
           SYHL S LPWMRV+KQ+S+L+SV CHRLSDPDAES   P PRASHSLNFVSDCLVLFGGG
Sbjct: 76  SYHLSSHLPWMRVFKQLSRLESVRCHRLSDPDAESSSLPGPRASHSLNFVSDCLVLFGGG 135

Query: 121 CEGGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIR 180
           CE GRHLDDTW+AYIGNDCHRML W+KMNSG PSGRFGHTC VVGDFLILFGGI DNGIR
Sbjct: 136 CESGRHLDDTWLAYIGNDCHRMLNWRKMNSGTPSGRFGHTCNVVGDFLILFGGINDNGIR 195

Query: 181 QNDTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRL 240
           QNDTW GR+ER  IHG  FSW  LDVG +APPPRGAHASCCID+KKMV+HGGIGLGGIRL
Sbjct: 196 QNDTWAGRVERKGIHGFEFSWIPLDVGELAPPPRGAHASCCIDDKKMVIHGGIGLGGIRL 255

Query: 241 LDTWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWL 300
            DTWVLELSE+LKFG+W+EIV+HPSPPARSGHTLTSIGG KVVLFGGRGVGYDVLND+WL
Sbjct: 256 FDTWVLELSENLKFGTWYEIVSHPSPPARSGHTLTSIGGMKVVLFGGRGVGYDVLNDIWL 315

Query: 301 LNIFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWAL 360
           L+IF+G  RWVQI+Y+LQN++EGVSLPRVGHSANLILGGRLLIYGGEDTERH KDDFWAL
Sbjct: 316 LSIFEGHGRWVQILYDLQNVTEGVSLPRVGHSANLILGGRLLIYGGEDTERHRKDDFWAL 375

Query: 361 DVGAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMV 420
           DV A+K+I E QLTT NS+GL+AKMWKRLKANG TP+SRSFHRAC DRSG  LY+LGGMV
Sbjct: 376 DVSALKTI-EVQLTTGNSIGLAAKMWKRLKANGCTPNSRSFHRACTDRSGCCLYILGGMV 435

Query: 421 DGLVLPAESSGLRFDGDLFLVELVI 444
           DG+V  AESSGLRFDGDLFLVELVI
Sbjct: 436 DGVVQAAESSGLRFDGDLFLVELVI 459

BLAST of MS009666 vs. NCBI nr
Match: XP_023548139.1 (F-box/kelch-repeat protein At1g51550 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 790.0 bits (2039), Expect = 1.0e-224
Identity = 376/446 (84.30%), Postives = 406/446 (91.03%), Query Frame = 0

Query: 1   SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 60
           SPITSLAPDHLF +LL LPVDSILSFS+TC RFRALTSSD LWEAICRREWGS+SVEAL 
Sbjct: 16  SPITSLAPDHLFAILLLLPVDSILSFSVTCSRFRALTSSDALWEAICRREWGSASVEALN 75

Query: 61  SYHL-SQLPWMRVYKQVSQLDSVYCHRLSDPDAESLH-PSPRASHSLNFVSDCLVLFGGG 120
           SYHL SQLPWMRV+KQ+S+L+SV CHRLSDP AES   P PRASHSLNFVSDCLVLFGGG
Sbjct: 76  SYHLSSQLPWMRVFKQLSRLESVRCHRLSDPVAESSSLPGPRASHSLNFVSDCLVLFGGG 135

Query: 121 CEGGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIR 180
           CE GRHLDDTW+AYIGNDCHRML W+KMNSG PSGRFGHTC VVGDFLILFGGI DNGIR
Sbjct: 136 CESGRHLDDTWLAYIGNDCHRMLNWRKMNSGTPSGRFGHTCNVVGDFLILFGGINDNGIR 195

Query: 181 QNDTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRL 240
           QNDTW GR+ER  IHG  FSW  LDVG +APPPRGAHASCCID+KKMV+HGGIGLGGIRL
Sbjct: 196 QNDTWAGRVERKGIHGFEFSWIPLDVGELAPPPRGAHASCCIDDKKMVIHGGIGLGGIRL 255

Query: 241 LDTWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWL 300
            DTWVLELSE+LKFG+WHEIV+HPSPPARSGHTLTSIGG KVVLFGGRGVGYDVLND+WL
Sbjct: 256 FDTWVLELSENLKFGTWHEIVSHPSPPARSGHTLTSIGGMKVVLFGGRGVGYDVLNDIWL 315

Query: 301 LNIFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWAL 360
           L+IF+G  RWVQI+Y+LQN++EGVSLPRVGHSANLILGGRLLIYGGEDTERH KDDFWAL
Sbjct: 316 LSIFEGHGRWVQILYDLQNVTEGVSLPRVGHSANLILGGRLLIYGGEDTERHRKDDFWAL 375

Query: 361 DVGAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMV 420
           DV A+K+I E QLTT NS+GL+AKMWKRLKANG TP+SRSFHRAC DRSGR LY+LGGMV
Sbjct: 376 DVSALKTI-EVQLTTGNSIGLAAKMWKRLKANGCTPNSRSFHRACTDRSGRCLYILGGMV 435

Query: 421 DGLV-LPAESSGLRFDGDLFLVELVI 444
           DG+V   AESSGLRFDGDLFLVELVI
Sbjct: 436 DGVVQAAAESSGLRFDGDLFLVELVI 460

BLAST of MS009666 vs. ExPASy Swiss-Prot
Match: Q9C8K7 (F-box/kelch-repeat protein At1g51550 OS=Arabidopsis thaliana OX=3702 GN=At1g51550 PE=2 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.4e-160
Identity = 272/454 (59.91%), Postives = 343/454 (75.55%), Query Frame = 0

Query: 1   SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 60
           SPI +L  DHL T+LL LPVDSILSFS+TC+R+++L  SD+LWEA+C REWG +SV+ALK
Sbjct: 19  SPIINLPDDHLLTILLLLPVDSILSFSMTCKRYKSLACSDSLWEALCEREWGPTSVDALK 78

Query: 61  SYHLSQ-LPWMRVYKQVSQLDSVYCHRLSDPDAE----SLHPSPRASHSLNFVSDCLVLF 120
              L     WM ++++V ++DSV CH++SDPD +    S  P PRASHSLNFV+D LVLF
Sbjct: 79  LSSLRDGFSWMLMFQRVYKMDSVCCHKISDPDDDDEESSSFPIPRASHSLNFVNDHLVLF 138

Query: 121 GGGCEGGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDN 180
           GGGC+GGRHLDDTW +Y+      +LKW+K+ SG PSGRFGHTC V+G++L+LFGGI D 
Sbjct: 139 GGGCQGGRHLDDTWTSYVDKSNQSILKWKKVKSGTPSGRFGHTCIVIGEYLLLFGGINDR 198

Query: 181 GIRQNDTWVGRIERSEIHGIGFSWRRLDVGVI---APPPRGAHASCCIDEKKMVVHGGIG 240
           G R NDTW+G++   E    G SW+ L+VG +    PPPRGAH++CCI EKKMVVHGGIG
Sbjct: 199 GERLNDTWIGQVFCHE----GLSWKLLNVGSLQRPRPPPRGAHSACCIAEKKMVVHGGIG 258

Query: 241 LGGIRLLDTWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDV 300
           L G+RL DTW+LELSED   G+WH + +   PP RSGHTLT I   +VVLFGGRG+GYDV
Sbjct: 259 LNGVRLGDTWILELSEDFSSGTWHMVESPQLPPPRSGHTLTCIRENQVVLFGGRGLGYDV 318

Query: 301 LNDVWLLNIFDG-QVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHH 360
           L+DVW+L+I +  + +W+QI Y+ Q++ E  SLPRVGHSA L+LGGR+LIYGGED+ RH 
Sbjct: 319 LDDVWILDIQEPCEEKWIQIFYDFQDVPEYASLPRVGHSATLVLGGRILIYGGEDSYRHR 378

Query: 361 KDDFWALDVGAMKS--IEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGR 420
           KDDFW LDV  + S  ++ Q L+ + S      +WK+L    Y P SRSFHRACAD SGR
Sbjct: 379 KDDFWVLDVKTIPSSGLKPQGLSLNGS-----SVWKKLDRISYGPKSRSFHRACADCSGR 438

Query: 421 YLYVLGGMVDGLVLPAESSGLRFDGDLFLVELVI 444
           +LYV GGMVDGL+ PA SSGLRFDG+LF+VELV+
Sbjct: 439 FLYVFGGMVDGLLQPAASSGLRFDGELFMVELVL 463

BLAST of MS009666 vs. ExPASy Swiss-Prot
Match: Q94BT6 (Adagio protein 1 OS=Arabidopsis thaliana OX=3702 GN=ADO1 PE=1 SV=2)

HSP 1 Score: 169.9 bits (429), Expect = 6.7e-41
Identity = 114/356 (32.02%), Postives = 184/356 (51.69%), Query Frame = 0

Query: 14  VLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALKSY-HLSQLPWMRV 73
           +L RL    + S S  CRR   LT ++ LW  +C+  WGS +   L++     +L W R+
Sbjct: 209 ILSRLTPRDVASVSSVCRRLYVLTKNEDLWRRVCQNAWGSETTRVLETVPGAKRLGWGRL 268

Query: 74  YKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCEGGRHLDDTWVAY 133
            ++++ L++    +LS     S+ PS R + S   V + +VLFGG     + ++DT+V  
Sbjct: 269 ARELTTLEAAAWRKLS--VGGSVEPS-RCNFSACAVGNRVVLFGGEGVNMQPMNDTFVLD 328

Query: 134 IGNDCHRMLKWQKMN-SGIPSGRFGHTCTVV-GDFLILFGGIKDNGIRQNDTWVGRIERS 193
           + +D     +WQ +  S  P GR+GHT T V G  L++FGG    G+  ND +V  ++  
Sbjct: 329 LNSD---YPEWQHVKVSSPPPGRWGHTLTCVNGSNLVVFGGCGQQGL-LNDVFVLNLDAK 388

Query: 194 EIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLDTWVLELSEDL 253
                  +WR +  G+  P PR  H+SC +D  K++V GG    G+ L DT++L+LS  +
Sbjct: 389 PP-----TWREIS-GLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLLSDTFLLDLS--I 448

Query: 254 KFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDV---LNDVWLLNIFDGQVR 313
           +   W EI A  +PP+R GHTL+  GG K+++FGG      +    +DV+ +++ + +  
Sbjct: 449 EKPVWREIPAAWTPPSRLGHTLSVYGGRKILMFGGLAKSGPLKFRSSDVFTMDLSEEEPC 508

Query: 314 WVQIVYE----LQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALD 360
           W  +         N       PR+ H A  + GGR+LI+GG     H     + LD
Sbjct: 509 WRCVTGSGMPGAGNPGGVAPPPRLDHVAVNLPGGRILIFGGSVAGLHSASQLYLLD 549

BLAST of MS009666 vs. ExPASy Swiss-Prot
Match: Q8W420 (Adagio protein 2 OS=Arabidopsis thaliana OX=3702 GN=ADO2 PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.9e-40
Identity = 121/411 (29.44%), Postives = 200/411 (48.66%), Query Frame = 0

Query: 14  VLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALKSY-HLSQLPWMRV 73
           +L +L    I S    CRR   LT +D +W  +C+  WG+ +   L+S     ++ W+R+
Sbjct: 210 ILSQLTPGDIASVGCVCRRLNELTKNDDVWRMVCQNTWGTEATRVLESVPGAKRIGWVRL 269

Query: 74  YKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCEGGRHLDDTWVAY 133
            ++ +  ++    + S     ++ PS R + S   V + +V+FGG     + ++DT+V  
Sbjct: 270 AREFTTHEATAWRKFS--VGGTVEPS-RCNFSACAVGNRIVIFGGEGVNMQPMNDTFVLD 329

Query: 134 IGNDCHRMLKWQK-MNSGIPSGRFGHTCTVV-GDFLILFGGIKDNGIRQNDTWVGRIERS 193
           +G+      +W+  + S  P GR+GHT + V G  L++FGG   +G+  ND ++  ++  
Sbjct: 330 LGSSSP---EWKSVLVSSPPPGRWGHTLSCVNGSRLVVFGGYGSHGL-LNDVFLLDLDAD 389

Query: 194 EIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLDTWVLELSEDL 253
                  SWR +  G+  P PR  H+SC +D  K++V GG    G  L DT++L+LS D+
Sbjct: 390 PP-----SWREVS-GLAPPIPRSWHSSCTLDGTKLIVSGGCADSGALLSDTFLLDLSMDI 449

Query: 254 KFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGG---RGVGYDVLNDVWLLNIFDGQVR 313
              +W EI    +PP+R GHTLT  G  K+++FGG    G      NDV+ +++ + +  
Sbjct: 450 P--AWREIPVPWTPPSRLGHTLTVYGDRKILMFGGLAKNGTLRFRSNDVYTMDLSEDEPS 509

Query: 314 WVQIVYELQNISEGVSL--PRVGHSANLILGGRLLIYGGEDTERHHKDDFWALDVGAMKS 373
           W  ++    ++  G++   PR+ H A  + GGR+LI+GG               V  + S
Sbjct: 510 WRPVIGYGSSLPGGMAAPPPRLDHVAISLPGGRILIFGG--------------SVAGLDS 569

Query: 374 IEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGG 417
             +  L   N        W+ L   G  P     H  C    G  L VLGG
Sbjct: 570 ASQLYLLDPNE---EKPAWRILNVQGGPPRFAWGHTTCV-VGGTRLVVLGG 587

BLAST of MS009666 vs. ExPASy Swiss-Prot
Match: Q5Z8K3 (Adagio-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0694000 PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 2.1e-39
Identity = 123/414 (29.71%), Postives = 202/414 (48.79%), Query Frame = 0

Query: 13  TVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALKSYHLS-QLPWMR 72
           ++L RL    I S S  CRR   LT ++ LW  +C+  WGS +  AL++   + +L W R
Sbjct: 229 SILSRLSPRDIASVSSVCRRLYLLTRNEDLWRMVCQNAWGSETTRALETVPAAKRLGWGR 288

Query: 73  VYKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCEGGRHLDDTWVA 132
           + ++++ L++V   +L+   A  + PS R + S   V + +VLFGG     + ++DT+V 
Sbjct: 289 LARELTTLEAVAWRKLTVGGA--VEPS-RCNFSACAVGNRVVLFGGEGVNMQPMNDTFVL 348

Query: 133 YIGNDCHRMLKWQKMN-SGIPSGRFGHTCTVV-GDFLILFGGIKDNGIRQNDTWVGRIER 192
            +        +W+ +N S  P GR+GHT + + G  L++FGG    G+  ND +   ++ 
Sbjct: 349 DLNAS---NPEWRHVNVSSAPPGRWGHTLSCLNGSLLVVFGGCGRQGL-LNDVFTLDLDA 408

Query: 193 SEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLDTWVLELSED 252
            +      +WR +  GV  P PR  H+SC +D  K+VV GG    G+ L DT++L+++ D
Sbjct: 409 KQP-----TWREIP-GVAPPVPRSWHSSCTLDGTKLVVSGGCADSGVLLSDTYLLDVTMD 468

Query: 253 LKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDV---LNDVWLLNIFDGQV 312
                W E+ A  +PP+R GH+++  GG K+++FGG      +    +DV+ +++ + + 
Sbjct: 469 KPV--WREVPASWTPPSRLGHSMSVYGGRKILMFGGLAKSGPLRLRSSDVFTMDLSEEEP 528

Query: 313 RWVQIVYE----LQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALDVGA 372
            W  +         N +     PR+ H A  + GGR+LI+GG     H     + LD   
Sbjct: 529 CWRCLTGSGMPGAGNPAGAGPPPRLDHVAVSLPGGRVLIFGGSVAGLHSASQLYLLD--- 588

Query: 373 MKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGG 417
               EE+              W+ L   G  P     H  C    G    VLGG
Sbjct: 589 --PTEEK------------PTWRILNVPGRPPRFAWGHSTCV-VGGTKAIVLGG 609

BLAST of MS009666 vs. ExPASy Swiss-Prot
Match: Q67UX0 (Putative adagio-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0150800 PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 2.0e-37
Identity = 122/413 (29.54%), Postives = 198/413 (47.94%), Query Frame = 0

Query: 14  VLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALKSY-HLSQLPWMRV 73
           +L RL    I S +  C+R   LT +D LW  +C+  WGS + + L++      L W R+
Sbjct: 223 ILSRLSPRDIASVNSVCKRLYHLTRNDDLWRMVCQNAWGSEATQVLETVAGTRSLAWGRL 282

Query: 74  YKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCEGGRHLDDTWVAY 133
            ++++ L++V   +L+   A  + PS R + S     + +VLFGG     + ++DT+V  
Sbjct: 283 ARELTTLEAVTWRKLTVGGA--VEPS-RCNFSACAAGNRVVLFGGEGVNMQPMNDTFVLD 342

Query: 134 IGNDCHRMLKWQKMN-SGIPSGRFGHTCTVV-GDFLILFGGIKDNGIRQNDTWVGRIERS 193
           +        +W+ +N    P GR+GHT + + G  L+LFGG    G+  ND ++  ++  
Sbjct: 343 LNAS---KPEWRHINVRSAPPGRWGHTLSCLNGSRLVLFGGCGRQGL-LNDVFMLDLDAQ 402

Query: 194 EIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLDTWVLELSEDL 253
           +      +WR +  G+  P PR  H+SC +D  K+VV GG    G+ L DT++L+++  +
Sbjct: 403 QP-----TWREIP-GLAPPVPRSWHSSCTLDGTKLVVSGGCADSGVLLSDTYLLDVT--M 462

Query: 254 KFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDV---LNDVWLLNIFDGQVR 313
           +   W EI A  +PP R GH+L+   G K+++FGG      +    NDV+ L++ + +  
Sbjct: 463 ERPVWREIPASWTPPCRLGHSLSVYDGRKILMFGGLAKSGPLRLRSNDVFTLDLSENKPC 522

Query: 314 WVQIVYE-LQNISEGVSL---PRVGHSANLILGGRLLIYGGEDTERHHKDDFWALDVGAM 373
           W  I    +   S    +   PR+ H A  + GGR+LI+GG     H     + LD    
Sbjct: 523 WRCITGSGMPGASNPAGVGPPPRLDHVAVSLPGGRILIFGGSVAGLHSASKLYLLD---- 582

Query: 374 KSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGG 417
              EE+              W+ L   G  P     H  C    G    VLGG
Sbjct: 583 -PTEEK------------PTWRILNVPGRPPRFAWGHSTCV-VGGTKAIVLGG 602

BLAST of MS009666 vs. ExPASy TrEMBL
Match: A0A6J1D7V5 (F-box/kelch-repeat protein At1g51550 OS=Momordica charantia OX=3673 GN=LOC111017836 PE=4 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 7.6e-266
Identity = 442/443 (99.77%), Postives = 442/443 (99.77%), Query Frame = 0

Query: 1   SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 60
           SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK
Sbjct: 18  SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 77

Query: 61  SYHLSQLPWMRVYKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCE 120
           SYHLSQLPWMRVYKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCE
Sbjct: 78  SYHLSQLPWMRVYKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCE 137

Query: 121 GGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIRQN 180
           GGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIRQN
Sbjct: 138 GGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIRQN 197

Query: 181 DTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLD 240
           DTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLD
Sbjct: 198 DTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLD 257

Query: 241 TWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWLLN 300
           TWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWLLN
Sbjct: 258 TWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWLLN 317

Query: 301 IFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALDV 360
           IFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALDV
Sbjct: 318 IFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALDV 377

Query: 361 GAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMVDG 420
            AMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMVDG
Sbjct: 378 SAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMVDG 437

Query: 421 LVLPAESSGLRFDGDLFLVELVI 444
           LVLPAESSGLRFDGDLFLVELVI
Sbjct: 438 LVLPAESSGLRFDGDLFLVELVI 460

BLAST of MS009666 vs. ExPASy TrEMBL
Match: A0A6J1JP99 (F-box/kelch-repeat protein At1g51550 OS=Cucurbita maxima OX=3661 GN=LOC111488627 PE=4 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.3e-225
Identity = 373/445 (83.82%), Postives = 405/445 (91.01%), Query Frame = 0

Query: 1   SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 60
           SPITSLAPDHLF +LL LPVDSILSFS+TC RFRALTSSD LWEAICRREWGS+SVEAL 
Sbjct: 16  SPITSLAPDHLFAILLLLPVDSILSFSVTCSRFRALTSSDALWEAICRREWGSASVEALN 75

Query: 61  SYHL-SQLPWMRVYKQVSQLDSVYCHRLSDPDAESLH-PSPRASHSLNFVSDCLVLFGGG 120
           SYHL S LPWMRV+KQ+S+L+SV CHRLSDPDAES   P PRASHSLNFVSDCLVLFGGG
Sbjct: 76  SYHLSSHLPWMRVFKQLSRLESVRCHRLSDPDAESSSLPGPRASHSLNFVSDCLVLFGGG 135

Query: 121 CEGGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIR 180
           CE GRHLDDTW+AYIGNDCHRML W+KMNSG PSGRFGHTC VVGDFLILFGGI DNGIR
Sbjct: 136 CESGRHLDDTWLAYIGNDCHRMLNWRKMNSGTPSGRFGHTCNVVGDFLILFGGINDNGIR 195

Query: 181 QNDTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRL 240
           QNDTW GR+ER  IHG  FSW  LDVG +APPPRGAHASCCID+KKMV+HGGIGLGGIRL
Sbjct: 196 QNDTWAGRVERKGIHGYEFSWIPLDVGELAPPPRGAHASCCIDDKKMVIHGGIGLGGIRL 255

Query: 241 LDTWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWL 300
            DTWVLELSE+LKFG+WHEIV+HPSPPARSGHTL SIGG KVVLFGGRGVGYDV+ND+WL
Sbjct: 256 FDTWVLELSENLKFGTWHEIVSHPSPPARSGHTLNSIGGMKVVLFGGRGVGYDVMNDIWL 315

Query: 301 LNIFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWAL 360
           L++F+G  RWVQI+Y+LQN++EGVSLPRVGHSANLILGGRLLIYGGEDTERH KDDFWAL
Sbjct: 316 LSLFEGHGRWVQILYDLQNVTEGVSLPRVGHSANLILGGRLLIYGGEDTERHRKDDFWAL 375

Query: 361 DVGAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMV 420
           DV A+K+I E QLTT NS+GL+AKMWKRLKANG TP+SRSFHRAC DRSGR LY+LGGMV
Sbjct: 376 DVSALKTI-EVQLTTGNSIGLAAKMWKRLKANGCTPNSRSFHRACTDRSGRCLYILGGMV 435

Query: 421 DGLVLPAESSGLRFDGDLFLVELVI 444
           DG+V  AESSGLRFDGDLFLVELVI
Sbjct: 436 DGVVQAAESSGLRFDGDLFLVELVI 459

BLAST of MS009666 vs. ExPASy TrEMBL
Match: A0A6J1GP12 (F-box/kelch-repeat protein At1g51550 OS=Cucurbita moschata OX=3662 GN=LOC111456148 PE=4 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 3.8e-225
Identity = 374/445 (84.04%), Postives = 405/445 (91.01%), Query Frame = 0

Query: 1   SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 60
           SPITSLAPDHLF +LL LPVDSILSFS+TC RFRALTSSD LWEAICRREWGS+SVEAL 
Sbjct: 16  SPITSLAPDHLFAILLLLPVDSILSFSVTCSRFRALTSSDALWEAICRREWGSASVEALN 75

Query: 61  SYHL-SQLPWMRVYKQVSQLDSVYCHRLSDPDAESLH-PSPRASHSLNFVSDCLVLFGGG 120
           SYHL S LPWMRV+KQ+S+L+SV CHRLSDPDAES   P PRASHSLNFVSDCLVLFGGG
Sbjct: 76  SYHLSSHLPWMRVFKQLSRLESVRCHRLSDPDAESSSLPGPRASHSLNFVSDCLVLFGGG 135

Query: 121 CEGGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIR 180
           CE GRHLDDTW+AYIGNDCHRML W+KMNSG PSGRFGHTC VVGDFLILFGGI DNGIR
Sbjct: 136 CESGRHLDDTWLAYIGNDCHRMLNWRKMNSGTPSGRFGHTCNVVGDFLILFGGINDNGIR 195

Query: 181 QNDTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRL 240
           QNDTW GR+ER  IHG  FSW  LDVG +APPPRGAHASCCID+KKMV+HGGIGLGGIRL
Sbjct: 196 QNDTWAGRVERKGIHGFEFSWIPLDVGELAPPPRGAHASCCIDDKKMVIHGGIGLGGIRL 255

Query: 241 LDTWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWL 300
            DTWVLELSE+LKFG+W+EIV+HPSPPARSGHTLTSIGG KVVLFGGRGVGYDVLND+WL
Sbjct: 256 FDTWVLELSENLKFGTWYEIVSHPSPPARSGHTLTSIGGMKVVLFGGRGVGYDVLNDIWL 315

Query: 301 LNIFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWAL 360
           L+IF+G  RWVQI+Y+LQN++EGVSLPRVGHSANLILGGRLLIYGGEDTERH KDDFWAL
Sbjct: 316 LSIFEGHGRWVQILYDLQNVTEGVSLPRVGHSANLILGGRLLIYGGEDTERHRKDDFWAL 375

Query: 361 DVGAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMV 420
           DV A+K+I E QLTT NS+GL+AKMWKRLKANG TP+SRSFHRAC DRSG  LY+LGGMV
Sbjct: 376 DVSALKTI-EVQLTTGNSIGLAAKMWKRLKANGCTPNSRSFHRACTDRSGCCLYILGGMV 435

Query: 421 DGLVLPAESSGLRFDGDLFLVELVI 444
           DG+V  AESSGLRFDGDLFLVELVI
Sbjct: 436 DGVVQAAESSGLRFDGDLFLVELVI 459

BLAST of MS009666 vs. ExPASy TrEMBL
Match: A0A6J5UCQ4 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS18529 PE=4 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 4.6e-194
Identity = 321/444 (72.30%), Postives = 372/444 (83.78%), Query Frame = 0

Query: 1   SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 60
           SPI+ +A DHLFT+LL LPVDSIL F++ C++FRALT+SDTLWE+ICRR+WG  SVEALK
Sbjct: 20  SPISYIAQDHLFTILLLLPVDSILCFAMACKKFRALTASDTLWESICRRDWGPKSVEALK 79

Query: 61  SYHL-SQLPWMRVYKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGC 120
           + +   QLPWMR+YKQVS+LDSVYCH+LS+PD E + PSPRASHSLNFVSDCLVLFGGG 
Sbjct: 80  ASNFHHQLPWMRLYKQVSRLDSVYCHKLSNPDGELVFPSPRASHSLNFVSDCLVLFGGGS 139

Query: 121 EGGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIRQ 180
           EGGRHLDDTWVAY+GND  RMLKWQK+NSGIPSGRFGH+C V+ DFL+LFGGI DNG R 
Sbjct: 140 EGGRHLDDTWVAYVGNDFRRMLKWQKINSGIPSGRFGHSCVVIDDFLVLFGGINDNGNRH 199

Query: 181 NDTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLL 240
           NDTWVG++   E  GI  SWR L VG  AP  RGAHA+CCID +KMV+HGGIGL G+RL 
Sbjct: 200 NDTWVGKVACHETLGITLSWRLLVVGPDAPSQRGAHAACCIDGRKMVIHGGIGLHGVRLG 259

Query: 241 DTWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWLL 300
           DTWVLELSE+ +FG+W+EIVAHP PPARSGHTLT IGGT+ VLFGGRG+GYDVL+DVW L
Sbjct: 260 DTWVLELSENFRFGTWYEIVAHPCPPARSGHTLTCIGGTRTVLFGGRGLGYDVLHDVWFL 319

Query: 301 NIFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALD 360
           +I DGQ +WVQI+YELQN+  GVSLPRVGHSA LILGG LLI GGED+ RH K+DFW LD
Sbjct: 320 DIHDGQPKWVQILYELQNVPGGVSLPRVGHSATLILGGHLLICGGEDSYRHRKNDFWVLD 379

Query: 361 VGAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMVD 420
           + A+ SI   Q TT NS+ L AKMWKRLK NGY P  RSFHRAC D SGRYL+V GGMVD
Sbjct: 380 ISAVPSI-TMQPTTLNSVQLLAKMWKRLKGNGYKPKCRSFHRACTDNSGRYLFVFGGMVD 439

Query: 421 GLVLPAESSGLRFDGDLFLVELVI 444
           G++ PA+ +GLRFDG+L LVELV+
Sbjct: 440 GVLQPADPAGLRFDGELVLVELVL 462

BLAST of MS009666 vs. ExPASy TrEMBL
Match: A0A251PU29 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G020600 PE=4 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 4.6e-194
Identity = 320/444 (72.07%), Postives = 373/444 (84.01%), Query Frame = 0

Query: 1   SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 60
           SPI+ +A DHLFT+LL LPVDSIL F++ C++FRALT+SDTLWE+ICRR+WG  SVEALK
Sbjct: 19  SPISYMAQDHLFTILLLLPVDSILCFAMACKKFRALTASDTLWESICRRDWGPKSVEALK 78

Query: 61  SYHL-SQLPWMRVYKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGC 120
           + +   QLPWMR+YKQVS+LDSVYCH+LS+PD E + PSPRASHSLNFVSDCLVLFGGG 
Sbjct: 79  ASNFHHQLPWMRLYKQVSRLDSVYCHKLSNPDGELVFPSPRASHSLNFVSDCLVLFGGGS 138

Query: 121 EGGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDNGIRQ 180
           EGGRHLDDTWVAY+GND  RMLKWQK+NSGIPSGRFGH+C V+ DFL+LFGGI DNG R 
Sbjct: 139 EGGRHLDDTWVAYVGNDFRRMLKWQKINSGIPSGRFGHSCVVIDDFLVLFGGINDNGSRH 198

Query: 181 NDTWVGRIERSEIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLL 240
           NDTWVG++   E  GI  SWR L VG  AP  RGAHA+CCID +KMV+HGGIGL G+RL 
Sbjct: 199 NDTWVGKVACHETLGITLSWRLLVVGPDAPSQRGAHAACCIDGRKMVIHGGIGLHGVRLG 258

Query: 241 DTWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDVLNDVWLL 300
           DTWVLELSE+ +FG+W+EIVAHP PPARSGHTLT IGGT+ VLFGGRG+GYDVL+D+W L
Sbjct: 259 DTWVLELSENFRFGTWYEIVAHPCPPARSGHTLTCIGGTRTVLFGGRGLGYDVLHDIWFL 318

Query: 301 NIFDGQVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALD 360
           +I +GQ +WVQI+YELQN+  GVSLPRVGHSA LILGG LLI GGED+ RH K+DFW LD
Sbjct: 319 DIHEGQPKWVQILYELQNVPGGVSLPRVGHSATLILGGHLLICGGEDSYRHRKNDFWVLD 378

Query: 361 VGAMKSIEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGGMVD 420
           + A+ SI   Q TT NS+ L AKMWKRLKANGY P  RSFHRAC D SGRYL+V GGMVD
Sbjct: 379 ISAVPSI-TMQPTTLNSVQLLAKMWKRLKANGYKPKCRSFHRACTDNSGRYLFVFGGMVD 438

Query: 421 GLVLPAESSGLRFDGDLFLVELVI 444
           G++ PA+ +GLRFDG+L LVELV+
Sbjct: 439 GVLQPADPAGLRFDGELVLVELVL 461

BLAST of MS009666 vs. TAIR 10
Match: AT1G51550.1 (Kelch repeat-containing F-box family protein )

HSP 1 Score: 567.4 bits (1461), Expect = 1.0e-161
Identity = 272/454 (59.91%), Postives = 343/454 (75.55%), Query Frame = 0

Query: 1   SPITSLAPDHLFTVLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALK 60
           SPI +L  DHL T+LL LPVDSILSFS+TC+R+++L  SD+LWEA+C REWG +SV+ALK
Sbjct: 19  SPIINLPDDHLLTILLLLPVDSILSFSMTCKRYKSLACSDSLWEALCEREWGPTSVDALK 78

Query: 61  SYHLSQ-LPWMRVYKQVSQLDSVYCHRLSDPDAE----SLHPSPRASHSLNFVSDCLVLF 120
              L     WM ++++V ++DSV CH++SDPD +    S  P PRASHSLNFV+D LVLF
Sbjct: 79  LSSLRDGFSWMLMFQRVYKMDSVCCHKISDPDDDDEESSSFPIPRASHSLNFVNDHLVLF 138

Query: 121 GGGCEGGRHLDDTWVAYIGNDCHRMLKWQKMNSGIPSGRFGHTCTVVGDFLILFGGIKDN 180
           GGGC+GGRHLDDTW +Y+      +LKW+K+ SG PSGRFGHTC V+G++L+LFGGI D 
Sbjct: 139 GGGCQGGRHLDDTWTSYVDKSNQSILKWKKVKSGTPSGRFGHTCIVIGEYLLLFGGINDR 198

Query: 181 GIRQNDTWVGRIERSEIHGIGFSWRRLDVGVI---APPPRGAHASCCIDEKKMVVHGGIG 240
           G R NDTW+G++   E    G SW+ L+VG +    PPPRGAH++CCI EKKMVVHGGIG
Sbjct: 199 GERLNDTWIGQVFCHE----GLSWKLLNVGSLQRPRPPPRGAHSACCIAEKKMVVHGGIG 258

Query: 241 LGGIRLLDTWVLELSEDLKFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDV 300
           L G+RL DTW+LELSED   G+WH + +   PP RSGHTLT I   +VVLFGGRG+GYDV
Sbjct: 259 LNGVRLGDTWILELSEDFSSGTWHMVESPQLPPPRSGHTLTCIRENQVVLFGGRGLGYDV 318

Query: 301 LNDVWLLNIFDG-QVRWVQIVYELQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHH 360
           L+DVW+L+I +  + +W+QI Y+ Q++ E  SLPRVGHSA L+LGGR+LIYGGED+ RH 
Sbjct: 319 LDDVWILDIQEPCEEKWIQIFYDFQDVPEYASLPRVGHSATLVLGGRILIYGGEDSYRHR 378

Query: 361 KDDFWALDVGAMKS--IEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGR 420
           KDDFW LDV  + S  ++ Q L+ + S      +WK+L    Y P SRSFHRACAD SGR
Sbjct: 379 KDDFWVLDVKTIPSSGLKPQGLSLNGS-----SVWKKLDRISYGPKSRSFHRACADCSGR 438

Query: 421 YLYVLGGMVDGLVLPAESSGLRFDGDLFLVELVI 444
           +LYV GGMVDGL+ PA SSGLRFDG+LF+VELV+
Sbjct: 439 FLYVFGGMVDGLLQPAASSGLRFDGELFMVELVL 463

BLAST of MS009666 vs. TAIR 10
Match: AT5G57360.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 169.9 bits (429), Expect = 4.7e-42
Identity = 114/356 (32.02%), Postives = 184/356 (51.69%), Query Frame = 0

Query: 14  VLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALKSY-HLSQLPWMRV 73
           +L RL    + S S  CRR   LT ++ LW  +C+  WGS +   L++     +L W R+
Sbjct: 209 ILSRLTPRDVASVSSVCRRLYVLTKNEDLWRRVCQNAWGSETTRVLETVPGAKRLGWGRL 268

Query: 74  YKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCEGGRHLDDTWVAY 133
            ++++ L++    +LS     S+ PS R + S   V + +VLFGG     + ++DT+V  
Sbjct: 269 ARELTTLEAAAWRKLS--VGGSVEPS-RCNFSACAVGNRVVLFGGEGVNMQPMNDTFVLD 328

Query: 134 IGNDCHRMLKWQKMN-SGIPSGRFGHTCTVV-GDFLILFGGIKDNGIRQNDTWVGRIERS 193
           + +D     +WQ +  S  P GR+GHT T V G  L++FGG    G+  ND +V  ++  
Sbjct: 329 LNSD---YPEWQHVKVSSPPPGRWGHTLTCVNGSNLVVFGGCGQQGL-LNDVFVLNLDAK 388

Query: 194 EIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLDTWVLELSEDL 253
                  +WR +  G+  P PR  H+SC +D  K++V GG    G+ L DT++L+LS  +
Sbjct: 389 PP-----TWREIS-GLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLLSDTFLLDLS--I 448

Query: 254 KFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDV---LNDVWLLNIFDGQVR 313
           +   W EI A  +PP+R GHTL+  GG K+++FGG      +    +DV+ +++ + +  
Sbjct: 449 EKPVWREIPAAWTPPSRLGHTLSVYGGRKILMFGGLAKSGPLKFRSSDVFTMDLSEEEPC 508

Query: 314 WVQIVYE----LQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALD 360
           W  +         N       PR+ H A  + GGR+LI+GG     H     + LD
Sbjct: 509 WRCVTGSGMPGAGNPGGVAPPPRLDHVAVNLPGGRILIFGGSVAGLHSASQLYLLD 549

BLAST of MS009666 vs. TAIR 10
Match: AT5G57360.2 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 169.9 bits (429), Expect = 4.7e-42
Identity = 114/356 (32.02%), Postives = 184/356 (51.69%), Query Frame = 0

Query: 14  VLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALKSY-HLSQLPWMRV 73
           +L RL    + S S  CRR   LT ++ LW  +C+  WGS +   L++     +L W R+
Sbjct: 209 ILSRLTPRDVASVSSVCRRLYVLTKNEDLWRRVCQNAWGSETTRVLETVPGAKRLGWGRL 268

Query: 74  YKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCEGGRHLDDTWVAY 133
            ++++ L++    +LS     S+ PS R + S   V + +VLFGG     + ++DT+V  
Sbjct: 269 ARELTTLEAAAWRKLS--VGGSVEPS-RCNFSACAVGNRVVLFGGEGVNMQPMNDTFVLD 328

Query: 134 IGNDCHRMLKWQKMN-SGIPSGRFGHTCTVV-GDFLILFGGIKDNGIRQNDTWVGRIERS 193
           + +D     +WQ +  S  P GR+GHT T V G  L++FGG    G+  ND +V  ++  
Sbjct: 329 LNSD---YPEWQHVKVSSPPPGRWGHTLTCVNGSNLVVFGGCGQQGL-LNDVFVLNLDAK 388

Query: 194 EIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLDTWVLELSEDL 253
                  +WR +  G+  P PR  H+SC +D  K++V GG    G+ L DT++L+LS  +
Sbjct: 389 PP-----TWREIS-GLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLLSDTFLLDLS--I 448

Query: 254 KFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGGRGVGYDV---LNDVWLLNIFDGQVR 313
           +   W EI A  +PP+R GHTL+  GG K+++FGG      +    +DV+ +++ + +  
Sbjct: 449 EKPVWREIPAAWTPPSRLGHTLSVYGGRKILMFGGLAKSGPLKFRSSDVFTMDLSEEEPC 508

Query: 314 WVQIVYE----LQNISEGVSLPRVGHSANLILGGRLLIYGGEDTERHHKDDFWALD 360
           W  +         N       PR+ H A  + GGR+LI+GG     H     + LD
Sbjct: 509 WRCVTGSGMPGAGNPGGVAPPPRLDHVAVNLPGGRILIFGGSVAGLHSASQLYLLD 549

BLAST of MS009666 vs. TAIR 10
Match: AT2G18915.1 (LOV KELCH protein 2 )

HSP 1 Score: 168.3 bits (425), Expect = 1.4e-41
Identity = 121/411 (29.44%), Postives = 200/411 (48.66%), Query Frame = 0

Query: 14  VLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALKSY-HLSQLPWMRV 73
           +L +L    I S    CRR   LT +D +W  +C+  WG+ +   L+S     ++ W+R+
Sbjct: 200 ILSQLTPGDIASVGCVCRRLNELTKNDDVWRMVCQNTWGTEATRVLESVPGAKRIGWVRL 259

Query: 74  YKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCEGGRHLDDTWVAY 133
            ++ +  ++    + S     ++ PS R + S   V + +V+FGG     + ++DT+V  
Sbjct: 260 AREFTTHEATAWRKFS--VGGTVEPS-RCNFSACAVGNRIVIFGGEGVNMQPMNDTFVLD 319

Query: 134 IGNDCHRMLKWQK-MNSGIPSGRFGHTCTVV-GDFLILFGGIKDNGIRQNDTWVGRIERS 193
           +G+      +W+  + S  P GR+GHT + V G  L++FGG   +G+  ND ++  ++  
Sbjct: 320 LGSSSP---EWKSVLVSSPPPGRWGHTLSCVNGSRLVVFGGYGSHGL-LNDVFLLDLDAD 379

Query: 194 EIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLDTWVLELSEDL 253
                  SWR +  G+  P PR  H+SC +D  K++V GG    G  L DT++L+LS D+
Sbjct: 380 PP-----SWREVS-GLAPPIPRSWHSSCTLDGTKLIVSGGCADSGALLSDTFLLDLSMDI 439

Query: 254 KFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGG---RGVGYDVLNDVWLLNIFDGQVR 313
              +W EI    +PP+R GHTLT  G  K+++FGG    G      NDV+ +++ + +  
Sbjct: 440 P--AWREIPVPWTPPSRLGHTLTVYGDRKILMFGGLAKNGTLRFRSNDVYTMDLSEDEPS 499

Query: 314 WVQIVYELQNISEGVSL--PRVGHSANLILGGRLLIYGGEDTERHHKDDFWALDVGAMKS 373
           W  ++    ++  G++   PR+ H A  + GGR+LI+GG               V  + S
Sbjct: 500 WRPVIGYGSSLPGGMAAPPPRLDHVAISLPGGRILIFGG--------------SVAGLDS 559

Query: 374 IEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGG 417
             +  L   N        W+ L   G  P     H  C    G  L VLGG
Sbjct: 560 ASQLYLLDPNE---EKPAWRILNVQGGPPRFAWGHTTCV-VGGTRLVVLGG 577

BLAST of MS009666 vs. TAIR 10
Match: AT2G18915.2 (LOV KELCH protein 2 )

HSP 1 Score: 168.3 bits (425), Expect = 1.4e-41
Identity = 121/411 (29.44%), Postives = 200/411 (48.66%), Query Frame = 0

Query: 14  VLLRLPVDSILSFSITCRRFRALTSSDTLWEAICRREWGSSSVEALKSY-HLSQLPWMRV 73
           +L +L    I S    CRR   LT +D +W  +C+  WG+ +   L+S     ++ W+R+
Sbjct: 210 ILSQLTPGDIASVGCVCRRLNELTKNDDVWRMVCQNTWGTEATRVLESVPGAKRIGWVRL 269

Query: 74  YKQVSQLDSVYCHRLSDPDAESLHPSPRASHSLNFVSDCLVLFGGGCEGGRHLDDTWVAY 133
            ++ +  ++    + S     ++ PS R + S   V + +V+FGG     + ++DT+V  
Sbjct: 270 AREFTTHEATAWRKFS--VGGTVEPS-RCNFSACAVGNRIVIFGGEGVNMQPMNDTFVLD 329

Query: 134 IGNDCHRMLKWQK-MNSGIPSGRFGHTCTVV-GDFLILFGGIKDNGIRQNDTWVGRIERS 193
           +G+      +W+  + S  P GR+GHT + V G  L++FGG   +G+  ND ++  ++  
Sbjct: 330 LGSSSP---EWKSVLVSSPPPGRWGHTLSCVNGSRLVVFGGYGSHGL-LNDVFLLDLDAD 389

Query: 194 EIHGIGFSWRRLDVGVIAPPPRGAHASCCIDEKKMVVHGGIGLGGIRLLDTWVLELSEDL 253
                  SWR +  G+  P PR  H+SC +D  K++V GG    G  L DT++L+LS D+
Sbjct: 390 PP-----SWREVS-GLAPPIPRSWHSSCTLDGTKLIVSGGCADSGALLSDTFLLDLSMDI 449

Query: 254 KFGSWHEIVAHPSPPARSGHTLTSIGGTKVVLFGG---RGVGYDVLNDVWLLNIFDGQVR 313
              +W EI    +PP+R GHTLT  G  K+++FGG    G      NDV+ +++ + +  
Sbjct: 450 P--AWREIPVPWTPPSRLGHTLTVYGDRKILMFGGLAKNGTLRFRSNDVYTMDLSEDEPS 509

Query: 314 WVQIVYELQNISEGVSL--PRVGHSANLILGGRLLIYGGEDTERHHKDDFWALDVGAMKS 373
           W  ++    ++  G++   PR+ H A  + GGR+LI+GG               V  + S
Sbjct: 510 WRPVIGYGSSLPGGMAAPPPRLDHVAISLPGGRILIFGG--------------SVAGLDS 569

Query: 374 IEEQQLTTSNSMGLSAKMWKRLKANGYTPSSRSFHRACADRSGRYLYVLGG 417
             +  L   N        W+ L   G  P     H  C    G  L VLGG
Sbjct: 570 ASQLYLLDPNE---EKPAWRILNVQGGPPRFAWGHTTCV-VGGTRLVVLGG 587

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149407.11.6e-26599.77F-box/kelch-repeat protein At1g51550 [Momordica charantia][more]
KAG7013930.19.4e-22684.04F-box/kelch-repeat protein [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022992252.12.7e-22583.82F-box/kelch-repeat protein At1g51550 [Cucurbita maxima] >XP_022992253.1 F-box/ke... [more]
XP_022953698.17.9e-22584.04F-box/kelch-repeat protein At1g51550 [Cucurbita moschata][more]
XP_023548139.11.0e-22484.30F-box/kelch-repeat protein At1g51550 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9C8K71.4e-16059.91F-box/kelch-repeat protein At1g51550 OS=Arabidopsis thaliana OX=3702 GN=At1g5155... [more]
Q94BT66.7e-4132.02Adagio protein 1 OS=Arabidopsis thaliana OX=3702 GN=ADO1 PE=1 SV=2[more]
Q8W4201.9e-4029.44Adagio protein 2 OS=Arabidopsis thaliana OX=3702 GN=ADO2 PE=1 SV=1[more]
Q5Z8K32.1e-3929.71Adagio-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0694000 P... [more]
Q67UX02.0e-3729.54Putative adagio-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g... [more]
Match NameE-valueIdentityDescription
A0A6J1D7V57.6e-26699.77F-box/kelch-repeat protein At1g51550 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1JP991.3e-22583.82F-box/kelch-repeat protein At1g51550 OS=Cucurbita maxima OX=3661 GN=LOC111488627... [more]
A0A6J1GP123.8e-22584.04F-box/kelch-repeat protein At1g51550 OS=Cucurbita moschata OX=3662 GN=LOC1114561... [more]
A0A6J5UCQ44.6e-19472.30Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS18529 PE=4 S... [more]
A0A251PU294.6e-19472.07Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G020600 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G51550.11.0e-16159.91Kelch repeat-containing F-box family protein [more]
AT5G57360.14.7e-4232.02Galactose oxidase/kelch repeat superfamily protein [more]
AT5G57360.24.7e-4232.02Galactose oxidase/kelch repeat superfamily protein [more]
AT2G18915.11.4e-4129.44LOV KELCH protein 2 [more]
AT2G18915.21.4e-4129.44LOV KELCH protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainSMARTSM00256fbox_2coord: 6..46
e-value: 0.0036
score: 26.5
IPR001810F-box domainPFAMPF00646F-boxcoord: 3..48
e-value: 6.5E-5
score: 22.7
IPR001810F-box domainPROSITEPS50181FBOXcoord: 1..46
score: 10.027357
NoneNo IPR availablePFAMPF13418Kelch_4coord: 211..265
e-value: 6.5E-5
score: 22.9
coord: 266..305
e-value: 4.2E-9
score: 36.3
coord: 153..184
e-value: 9.3E-6
score: 25.6
NoneNo IPR availableGENE3D1.20.1280.50coord: 1..52
e-value: 3.5E-6
score: 28.6
NoneNo IPR availablePANTHERPTHR46175:SF4BACTERIOOPSIN TRANSCRIPTIONAL ACTIVATORcoord: 1..442
NoneNo IPR availablePANTHERPTHR46175BACTERIOOPSIN TRANSCRIPTIONAL ACTIVATORcoord: 1..442
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 53..199
e-value: 1.6E-15
score: 58.9
coord: 200..421
e-value: 5.5E-31
score: 109.7
IPR015915Kelch-type beta propellerSUPERFAMILY117281Kelch motifcoord: 95..359
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 2..76

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009666.1MS009666.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding