MS009388 (gene) Bitter gourd (TR) v1

Overview
NameMS009388
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRNA helicase
Locationscaffold813: 891876 .. 894009 (+)
RNA-Seq ExpressionMS009388
SyntenyMS009388
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTACAAATGAAGGACGAAAAGAACATAGAGGAGGAAGATGAATATGTTGAATATGTTCCCGTGGCAAAGCGTAGAGCATTGGAAGCTCAAAAGATTCTTATGCGCAAGGGAAAGGCTTCTGCTCTTGAGGAAGAGTTGGAGAAGTCAAGGCTTGCTGAAGCCAAGCCCAGTCTCCTTATAAAGGCATCTCAGATGAAGCGTGACCAGCCCGAGGTCAGTCCGACCGAGCAGATTGTTCAGCAAGAAAAGGAGATGATTGAGCACTTATCTGATCGAAAAACTCTAATGTCTGTTCGGGAACTAGCAAAAGGAATAACTTACACGGAGCCACTGTTAACTGGATGGAAGCCACCATTGCCTATCAGGAGGATGTCGAGAAAGGCGTGTGATTTGATTCGCAAACAATGGCACATAATAGTTGATGGTGATGATATTCCACCGCCAATTAAAAACTTCAAGGACATGAGACTGCCAGAACCAATTTTGAAGAAGTTGAAAGAAAAGGGAATTGTGCAGCCTACTCCCATTCAGGTCCAAGGTCTTCCTGTGATTTTATCTGGCAGAGATATGATCGGGATTGCATTTACGGGCTCGGGAAAGACCCTGGTTTTTGTTCTTCCTTTAATAATGATTGCACTACAAGAAGAGGTCATGATGCCCATAGTTTCAGGAGAAGGGCCTTTCGGTTTGATTATTTGCCCTTCAAGGGAGCTTGCAAGACAGACTTATGAAGTGGTCGAACAGTTTTTAATTCCCATGAAGGAGGCTGGATATCCAGAACTAAGGCCTTTACTTTGTATTGGTGGAGTGGATATGAGATCACAAATCGAGGTGGTGAAGAAGGGTGTTCATATTGTTGTTGCAACTCCTGGGAGGTTGAAGGATATGTTGGCAAAAAAGAAAATGAACCTTGACAATTGCAGGTAAAAGATTTTCTCCATTTTCTATCTCATCATTTGGGTTGGACTCTCTGAAAAGAAAACTGAAATGGACTCATCAATGATAGCAAGAAGTAATGAAGGTTTTTGATAATGTTCGTATATTTTGTGGAAATTGTTATTTGTTTGGCAAAGAAAAATCCATGCTTGATTGCGTCCTCTAGGATCTTTTCCCCTAAAGTTATTTTTGATTATTTGTTGTTAAGCCTCTACTCATTTCCCATTACTTCAGATGATGCAAATTGATGATTGTTGTTTTAACCTTCTGACTTTAGTGGTTCTCCTCTTGGTTGGGAATAATGATATACCAATTTTTCTATTGCCACAGGTATCTTACTTTAGACGAGGCTGATAGATTAGTGGATCTGGGATTTGAAGACGATATTAGGGAAGTTTTTGATCATTTCAAGGCTCAACGCCAAACTCTCTTATTCTCTGCTACAATGCCTACCAAAATTCAAAATTTTGCAAGAAGTGCCCTGGTAAAGCCTGTAACCGTCAATGTGGGAAGAGCTGGAGCTGCAAACCTTGACGTCATCCAGGAAGTCGAGTATGTGAAACAAGAGGCAAAAATTGTTTACTTGCTTGAGTGCCTTCAGAAAACTCCACCTCCGGTGTTGATATTTTGCGAGAACAAAGCCGATGTTGACGACATCCATGAGTACCTCCTCTTGAAAGGTGTGGAAGCAGTGGCAATTCATGGAGGGAAAGATCAGGAAGAGAGAGAGTATGCTATATCATCCTTCAAGGCGGGAAAGAAAGATGTATTGGTTGCAACTGACGTTGCATCAAAGGGTTTAGATTTCCCAGACATTCAACATGTTATCAACTATGACATGCCAGCCGAAATTGAGAACTATGTCCATCGGATTGGTCGAACTGGACGGTGTGGTAAGACGGGAATCGCAACAACTTTCATAAACAAGAATCAAAGCGAAACGACTCTCCTCGATTTGAAACACCTTCTGCAAGAAGCAAAACAGAGGATTCCACCAGTGTTGGCCGAGCTGAATGACCCGATGGAGGATGTGGAAGCGATCACGAACGCAAGTGGGGTCAAAGGTTGTGCTTACTGTGGTGGACTTGGTCACCGTATCCGTGACTGCCCCAAATTAGAGCATCAAAAAAGCATGGCAATTGCCAGTTCTAGAAGAGATTACTTTGGATCAGGTGGTTATAGAGGAGAAATT

mRNA sequence

ATGGATGTACAAATGAAGGACGAAAAGAACATAGAGGAGGAAGATGAATATGTTGAATATGTTCCCGTGGCAAAGCGTAGAGCATTGGAAGCTCAAAAGATTCTTATGCGCAAGGGAAAGGCTTCTGCTCTTGAGGAAGAGTTGGAGAAGTCAAGGCTTGCTGAAGCCAAGCCCAGTCTCCTTATAAAGGCATCTCAGATGAAGCGTGACCAGCCCGAGGTCAGTCCGACCGAGCAGATTGTTCAGCAAGAAAAGGAGATGATTGAGCACTTATCTGATCGAAAAACTCTAATGTCTGTTCGGGAACTAGCAAAAGGAATAACTTACACGGAGCCACTGTTAACTGGATGGAAGCCACCATTGCCTATCAGGAGGATGTCGAGAAAGGCGTGTGATTTGATTCGCAAACAATGGCACATAATAGTTGATGGTGATGATATTCCACCGCCAATTAAAAACTTCAAGGACATGAGACTGCCAGAACCAATTTTGAAGAAGTTGAAAGAAAAGGGAATTGTGCAGCCTACTCCCATTCAGGTCCAAGGTCTTCCTGTGATTTTATCTGGCAGAGATATGATCGGGATTGCATTTACGGGCTCGGGAAAGACCCTGGTTTTTGTTCTTCCTTTAATAATGATTGCACTACAAGAAGAGGTCATGATGCCCATAGTTTCAGGAGAAGGGCCTTTCGGTTTGATTATTTGCCCTTCAAGGGAGCTTGCAAGACAGACTTATGAAGTGGTCGAACAGTTTTTAATTCCCATGAAGGAGGCTGGATATCCAGAACTAAGGCCTTTACTTTGTATTGGTGGAGTGGATATGAGATCACAAATCGAGGTGGTGAAGAAGGGTGTTCATATTGTTGTTGCAACTCCTGGGAGGTTGAAGGATATGTTGGCAAAAAAGAAAATGAACCTTGACAATTGCAGGTATCTTACTTTAGACGAGGCTGATAGATTAGTGGATCTGGGATTTGAAGACGATATTAGGGAAGTTTTTGATCATTTCAAGGCTCAACGCCAAACTCTCTTATTCTCTGCTACAATGCCTACCAAAATTCAAAATTTTGCAAGAAGTGCCCTGGTAAAGCCTGTAACCGTCAATGTGGGAAGAGCTGGAGCTGCAAACCTTGACGTCATCCAGGAAGTCGAGTATGTGAAACAAGAGGCAAAAATTGTTTACTTGCTTGAGTGCCTTCAGAAAACTCCACCTCCGGTGTTGATATTTTGCGAGAACAAAGCCGATGTTGACGACATCCATGAGTACCTCCTCTTGAAAGGTGTGGAAGCAGTGGCAATTCATGGAGGGAAAGATCAGGAAGAGAGAGAGTATGCTATATCATCCTTCAAGGCGGGAAAGAAAGATGTATTGGTTGCAACTGACGTTGCATCAAAGGGTTTAGATTTCCCAGACATTCAACATGTTATCAACTATGACATGCCAGCCGAAATTGAGAACTATGTCCATCGGATTGGTCGAACTGGACGGTGTGGTAAGACGGGAATCGCAACAACTTTCATAAACAAGAATCAAAGCGAAACGACTCTCCTCGATTTGAAACACCTTCTGCAAGAAGCAAAACAGAGGATTCCACCAGTGTTGGCCGAGCTGAATGACCCGATGGAGGATGTGGAAGCGATCACGAACGCAAGTGGGGTCAAAGGTTGTGCTTACTGTGGTGGACTTGGTCACCGTATCCGTGACTGCCCCAAATTAGAGCATCAAAAAAGCATGGCAATTGCCAGTTCTAGAAGAGATTACTTTGGATCAGGTGGTTATAGAGGAGAAATT

Coding sequence (CDS)

ATGGATGTACAAATGAAGGACGAAAAGAACATAGAGGAGGAAGATGAATATGTTGAATATGTTCCCGTGGCAAAGCGTAGAGCATTGGAAGCTCAAAAGATTCTTATGCGCAAGGGAAAGGCTTCTGCTCTTGAGGAAGAGTTGGAGAAGTCAAGGCTTGCTGAAGCCAAGCCCAGTCTCCTTATAAAGGCATCTCAGATGAAGCGTGACCAGCCCGAGGTCAGTCCGACCGAGCAGATTGTTCAGCAAGAAAAGGAGATGATTGAGCACTTATCTGATCGAAAAACTCTAATGTCTGTTCGGGAACTAGCAAAAGGAATAACTTACACGGAGCCACTGTTAACTGGATGGAAGCCACCATTGCCTATCAGGAGGATGTCGAGAAAGGCGTGTGATTTGATTCGCAAACAATGGCACATAATAGTTGATGGTGATGATATTCCACCGCCAATTAAAAACTTCAAGGACATGAGACTGCCAGAACCAATTTTGAAGAAGTTGAAAGAAAAGGGAATTGTGCAGCCTACTCCCATTCAGGTCCAAGGTCTTCCTGTGATTTTATCTGGCAGAGATATGATCGGGATTGCATTTACGGGCTCGGGAAAGACCCTGGTTTTTGTTCTTCCTTTAATAATGATTGCACTACAAGAAGAGGTCATGATGCCCATAGTTTCAGGAGAAGGGCCTTTCGGTTTGATTATTTGCCCTTCAAGGGAGCTTGCAAGACAGACTTATGAAGTGGTCGAACAGTTTTTAATTCCCATGAAGGAGGCTGGATATCCAGAACTAAGGCCTTTACTTTGTATTGGTGGAGTGGATATGAGATCACAAATCGAGGTGGTGAAGAAGGGTGTTCATATTGTTGTTGCAACTCCTGGGAGGTTGAAGGATATGTTGGCAAAAAAGAAAATGAACCTTGACAATTGCAGGTATCTTACTTTAGACGAGGCTGATAGATTAGTGGATCTGGGATTTGAAGACGATATTAGGGAAGTTTTTGATCATTTCAAGGCTCAACGCCAAACTCTCTTATTCTCTGCTACAATGCCTACCAAAATTCAAAATTTTGCAAGAAGTGCCCTGGTAAAGCCTGTAACCGTCAATGTGGGAAGAGCTGGAGCTGCAAACCTTGACGTCATCCAGGAAGTCGAGTATGTGAAACAAGAGGCAAAAATTGTTTACTTGCTTGAGTGCCTTCAGAAAACTCCACCTCCGGTGTTGATATTTTGCGAGAACAAAGCCGATGTTGACGACATCCATGAGTACCTCCTCTTGAAAGGTGTGGAAGCAGTGGCAATTCATGGAGGGAAAGATCAGGAAGAGAGAGAGTATGCTATATCATCCTTCAAGGCGGGAAAGAAAGATGTATTGGTTGCAACTGACGTTGCATCAAAGGGTTTAGATTTCCCAGACATTCAACATGTTATCAACTATGACATGCCAGCCGAAATTGAGAACTATGTCCATCGGATTGGTCGAACTGGACGGTGTGGTAAGACGGGAATCGCAACAACTTTCATAAACAAGAATCAAAGCGAAACGACTCTCCTCGATTTGAAACACCTTCTGCAAGAAGCAAAACAGAGGATTCCACCAGTGTTGGCCGAGCTGAATGACCCGATGGAGGATGTGGAAGCGATCACGAACGCAAGTGGGGTCAAAGGTTGTGCTTACTGTGGTGGACTTGGTCACCGTATCCGTGACTGCCCCAAATTAGAGCATCAAAAAAGCATGGCAATTGCCAGTTCTAGAAGAGATTACTTTGGATCAGGTGGTTATAGAGGAGAAATT

Protein sequence

MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSLLIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPPLPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Homology
BLAST of MS009388 vs. NCBI nr
Match: XP_022148436.1 (DEAD-box ATP-dependent RNA helicase 35 [Momordica charantia])

HSP 1 Score: 1171.8 bits (3030), Expect = 0.0e+00
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0

Query: 1   MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL 60
           MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 1   MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL 60

Query: 61  LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120
           LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 61  LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120

Query: 121 LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV 180
           LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV
Sbjct: 121 LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV 180

Query: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240

Query: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA
Sbjct: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300

Query: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360

Query: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420

Query: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM 480
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM 480

Query: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540

Query: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 597

BLAST of MS009388 vs. NCBI nr
Match: XP_004135864.1 (DEAD-box ATP-dependent RNA helicase 35 [Cucumis sativus] >KAE8646435.1 hypothetical protein Csa_015670 [Cucumis sativus])

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 588/597 (98.49%), Postives = 595/597 (99.66%), Query Frame = 0

Query: 1   MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL 60
           MDVQMKDEK++EEEDEYVEYVPVAKRRA+EAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 1   MDVQMKDEKSLEEEDEYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 60

Query: 61  LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120
           L+KASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 61  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120

Query: 121 LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV 180
           LPIRRM +KACDLIRKQWHIIVDGD+IPPPIKNFKDMRLPEP+LKKLKEKGIVQPTPIQV
Sbjct: 121 LPIRRMPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 180

Query: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240

Query: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA
Sbjct: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300

Query: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360

Query: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420

Query: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM 480
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 480

Query: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540

Query: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 597

BLAST of MS009388 vs. NCBI nr
Match: XP_008461142.1 (PREDICTED: DEAD-box ATP-dependent RNA helicase 35 [Cucumis melo] >XP_008461143.1 PREDICTED: DEAD-box ATP-dependent RNA helicase 35 [Cucumis melo] >KAA0058735.1 DEAD-box ATP-dependent RNA helicase 35 [Cucumis melo var. makuwa])

HSP 1 Score: 1159.4 bits (2998), Expect = 0.0e+00
Identity = 587/597 (98.32%), Postives = 595/597 (99.66%), Query Frame = 0

Query: 1   MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL 60
           MDVQMKDEK++EEED+YVEYVPVAKRRA+EAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 1   MDVQMKDEKSLEEEDDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 60

Query: 61  LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120
           L+KASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 61  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120

Query: 121 LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV 180
           LPIRRM +KACDLIRKQWHIIVDGD+IPPPIKNFKDMRLPEP+LKKLKEKGIVQPTPIQV
Sbjct: 121 LPIRRMPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 180

Query: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240

Query: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA
Sbjct: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300

Query: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360

Query: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420

Query: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM 480
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 480

Query: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540

Query: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 597

BLAST of MS009388 vs. NCBI nr
Match: XP_023004285.1 (DEAD-box ATP-dependent RNA helicase 35 [Cucurbita maxima] >XP_023004286.1 DEAD-box ATP-dependent RNA helicase 35 [Cucurbita maxima] >XP_023514207.1 DEAD-box ATP-dependent RNA helicase 35 [Cucurbita pepo subsp. pepo] >KAG7025731.1 DEAD-box ATP-dependent RNA helicase 35 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 586/597 (98.16%), Postives = 594/597 (99.50%), Query Frame = 0

Query: 1   MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL 60
           MDV MKDEK++EEE++YVEYVPVAKRRA+EAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 2   MDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 61

Query: 61  LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120
           L+KASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 62  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 121

Query: 121 LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV 180
           LPIRRM RKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEP+LKKLKEKGIVQPTPIQV
Sbjct: 122 LPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 181

Query: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 182 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 241

Query: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIE+VKKGVHIVVATPGRLKDMLA
Sbjct: 242 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLA 301

Query: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 302 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 361

Query: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 362 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 421

Query: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM 480
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 422 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 481

Query: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 482 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 541

Query: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 542 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598

BLAST of MS009388 vs. NCBI nr
Match: KAG6593383.1 (DEAD-box ATP-dependent RNA helicase 35, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 586/597 (98.16%), Postives = 594/597 (99.50%), Query Frame = 0

Query: 1   MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL 60
           MDV MKDEK++EEE++YVEYVPVAKRRA+EAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 74  MDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 133

Query: 61  LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120
           L+KASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 134 LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 193

Query: 121 LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV 180
           LPIRRM RKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEP+LKKLKEKGIVQPTPIQV
Sbjct: 194 LPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 253

Query: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 254 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 313

Query: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIE+VKKGVHIVVATPGRLKDMLA
Sbjct: 314 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLA 373

Query: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 374 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 433

Query: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 434 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 493

Query: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM 480
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 494 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 553

Query: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 554 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 613

Query: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 614 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 670

BLAST of MS009388 vs. ExPASy Swiss-Prot
Match: Q9LU46 (DEAD-box ATP-dependent RNA helicase 35 OS=Arabidopsis thaliana OX=3702 GN=RH35 PE=2 SV=1)

HSP 1 Score: 1041.6 bits (2692), Expect = 3.5e-303
Identity = 522/590 (88.47%), Postives = 557/590 (94.41%), Query Frame = 0

Query: 8   EKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSLLIKASQM 67
           E  +EE D Y+EYV VA+RRA+ AQKIL RKGKAS LEEE +K +LAEAKPSLL++A+Q+
Sbjct: 2   ESIMEEADSYIEYVSVAERRAIAAQKILQRKGKASELEEEADKEKLAEAKPSLLVQATQL 61

Query: 68  KRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPPLPIRRMS 127
           KRD PEVS TEQI+ QEKEM+EHLSD+KTLMSVRELAKGITYTEPLLTGWKPPL IR+MS
Sbjct: 62  KRDVPEVSATEQIILQEKEMMEHLSDKKTLMSVRELAKGITYTEPLLTGWKPPLHIRKMS 121

Query: 128 RKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQVQGLPVIL 187
            K  DLIRKQWHIIV+GDDIPPPIKNFKDM+ P P+L  LKEKGIVQPTPIQVQGLPVIL
Sbjct: 122 SKQRDLIRKQWHIIVNGDDIPPPIKNFKDMKFPRPVLDTLKEKGIVQPTPIQVQGLPVIL 181

Query: 188 SGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEV 247
           +GRDMIGIAFTGSGKTLVFVLP+IMIALQEE+MMPI +GEGP GLI+CPSRELARQTYEV
Sbjct: 182 AGRDMIGIAFTGSGKTLVFVLPMIMIALQEEMMMPIAAGEGPIGLIVCPSRELARQTYEV 241

Query: 248 VEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAKKKMNLD 307
           VEQF+ P+ EAGYP LR LLCIGG+DMRSQ+EVVK+GVHIVVATPGRLKDMLAKKKM+LD
Sbjct: 242 VEQFVAPLVEAGYPPLRSLLCIGGIDMRSQLEVVKRGVHIVVATPGRLKDMLAKKKMSLD 301

Query: 308 NCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTV 367
            CRYLTLDEADRLVDLGFEDDIREVFDHFK+QRQTLLFSATMPTKIQ FARSALVKPVTV
Sbjct: 302 ACRYLTLDEADRLVDLGFEDDIREVFDHFKSQRQTLLFSATMPTKIQIFARSALVKPVTV 361

Query: 368 NVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKG 427
           NVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKT PPVLIFCENKADVDDIHEYLLLKG
Sbjct: 362 NVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTSPPVLIFCENKADVDDIHEYLLLKG 421

Query: 428 VEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENY 487
           VEAVAIHGGKDQE+REYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENY
Sbjct: 422 VEAVAIHGGKDQEDREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENY 481

Query: 488 VHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAI 547
           VHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPME+ E I
Sbjct: 482 VHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEEAETI 541

Query: 548 TNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
            NASGVKGCAYCGGLGHRIRDCPKLEHQKS+AI++SR+DYFGSGGYRGEI
Sbjct: 542 ANASGVKGCAYCGGLGHRIRDCPKLEHQKSVAISNSRKDYFGSGGYRGEI 591

BLAST of MS009388 vs. ExPASy Swiss-Prot
Match: Q0E3X4 (DEAD-box ATP-dependent RNA helicase 35A OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0150100 PE=2 SV=2)

HSP 1 Score: 977.2 bits (2525), Expect = 8.1e-284
Identity = 488/612 (79.74%), Postives = 544/612 (88.89%), Query Frame = 0

Query: 10  NIEEEDEYVEYVPVAKRRALEAQKI-LMRKGK-----ASALEEELE-------------- 69
           N ++ED Y EY+PVAKRRA+EA ++  +R  K     +SA E   +              
Sbjct: 16  NSDDEDNYEEYIPVAKRRAMEADRLRRLRLSKPAPPSSSAAEAASDLPPPPPPPPNQPSA 75

Query: 70  ----KSRLAEAKPSLLIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAK 129
                   A AKPSLL+KA+Q+KR  PEV+ TEQ++ QEKEMIEHLSDRKTLMSVRELAK
Sbjct: 76  GGGGGGLEASAKPSLLVKATQLKRAAPEVTHTEQLIMQEKEMIEHLSDRKTLMSVRELAK 135

Query: 130 GITYTEPLLTGWKPPLPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILK 189
           GITY++PL TGWKPPL +RRM R   D +R++WHI+VDGDD+PPP ++F+D+RLPEP+L+
Sbjct: 136 GITYSDPLKTGWKPPLRLRRMPRAKADELRRKWHILVDGDDVPPPARDFRDLRLPEPMLR 195

Query: 190 KLKEKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS 249
           KL+EKGIVQPTPIQVQGLPV+LSGRDMIGIAFTGSGKTLVFVLPLIM+ALQEE+MMPIV 
Sbjct: 196 KLREKGIVQPTPIQVQGLPVVLSGRDMIGIAFTGSGKTLVFVLPLIMVALQEEMMMPIVP 255

Query: 250 GEGPFGLIICPSRELARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGV 309
           GEGPFG+IICPSRELA+QTY+V+EQFL+P+KEAGYPE+RPLLCIGGVDMR+Q++VVKKGV
Sbjct: 256 GEGPFGMIICPSRELAKQTYDVIEQFLVPLKEAGYPEIRPLLCIGGVDMRAQLDVVKKGV 315

Query: 310 HIVVATPGRLKDMLAKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLF 369
           HIVVATPGRLKD+LAKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLF
Sbjct: 316 HIVVATPGRLKDLLAKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLF 375

Query: 370 SATMPTKIQNFARSALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPP 429
           SATMP KIQNFA+SALVKPV VNVGRAGAANLDVIQEVEYVK++A+I+YLLECLQKTPPP
Sbjct: 376 SATMPKKIQNFAKSALVKPVIVNVGRAGAANLDVIQEVEYVKEDARIIYLLECLQKTPPP 435

Query: 430 VLIFCENKADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASK 489
           VL+FCENKADVD IHEYLLLKGVEAVAIHGGKDQEERE AI  FK GKKDVLVATDVASK
Sbjct: 436 VLVFCENKADVDYIHEYLLLKGVEAVAIHGGKDQEERENAIEFFKNGKKDVLVATDVASK 495

Query: 490 GLDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQE 549
           GLDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQ+ETTLLDLKHLL+E
Sbjct: 496 GLDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQTETTLLDLKHLLKE 555

Query: 550 AKQRIPPVLAELNDPMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRR 598
           AKQRIPPVLAELNDP+ED E +   SGVKGCAYCGGLGHR+ DCPKLEHQKSMAIA SRR
Sbjct: 556 AKQRIPPVLAELNDPLEDEETMAKESGVKGCAYCGGLGHRVTDCPKLEHQKSMAIAGSRR 615

BLAST of MS009388 vs. ExPASy Swiss-Prot
Match: Q5Z6G5 (DEAD-box ATP-dependent RNA helicase 35B OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0697200 PE=3 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 9.0e-259
Identity = 450/599 (75.13%), Postives = 518/599 (86.48%), Query Frame = 0

Query: 13  EEDEYVEYVPVAKRRALEAQKILMRKGK-------ASALEEELEKSRLAEA------KPS 72
           +E++Y EYVPVAKRRA+EA++ L R  K       A A      +S  + A      K S
Sbjct: 22  DEEDYEEYVPVAKRRAMEAER-LRRATKPPTTNAVAVAAPPPPPRSTSSPAVGEVAVKTS 81

Query: 73  LLIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKP 132
           LL+KA+++KR+ PEV+P E+++QQE+EMIEHLSDRK LM V E+AKGI+Y+EP+ TGW+P
Sbjct: 82  LLVKATKLKREAPEVTPAERLLQQEREMIEHLSDRKALMPVGEIAKGISYSEPITTGWRP 141

Query: 133 PLPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQ 192
           PL +RRM R   D +R+ WHI+VDGDD+PPP ++F D+RLPEPIL+ L+ KGI +PTPIQ
Sbjct: 142 PLRLRRMPRSRADALRRSWHILVDGDDVPPPSRSFGDLRLPEPILRALRGKGIEKPTPIQ 201

Query: 193 VQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRE 252
           VQGLPV LSGRDMIGIAFTGSGKTLVFVLPLIM ALQEE++MPIV GEGPFGLI+CPSRE
Sbjct: 202 VQGLPVALSGRDMIGIAFTGSGKTLVFVLPLIMAALQEEILMPIVPGEGPFGLIVCPSRE 261

Query: 253 LARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDML 312
           LARQT+EV+E FL P+ EAGYPE+RPLLCIGGVDMR+Q+EVVKKGVHIVVATPGRLKD+L
Sbjct: 262 LARQTHEVIEMFLAPLMEAGYPEIRPLLCIGGVDMRTQMEVVKKGVHIVVATPGRLKDLL 321

Query: 313 AKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARS 372
           +KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMP KIQNFA+S
Sbjct: 322 SKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPEKIQNFAKS 381

Query: 373 ALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDI 432
           ALVKP+ VNVGRAGAANLDVIQEVEYVK+EA+I+YLLECLQKTPPPVL+FCE+KADVD I
Sbjct: 382 ALVKPIIVNVGRAGAANLDVIQEVEYVKEEARIIYLLECLQKTPPPVLVFCEHKADVDYI 441

Query: 433 HEYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYD 492
            E+LLLKGVEAVAIHGGKD EER+ A  SFKA +KDVLVATDVASKGLD PDIQHVINYD
Sbjct: 442 QEFLLLKGVEAVAIHGGKDDEERKDAFKSFKASEKDVLVATDVASKGLDIPDIQHVINYD 501

Query: 493 MPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELND 552
           MPAEIENYVHRIGRTGR GKTG+ATTFINKNQ+ETTLLDLK LL E+KQR+PP+LA+L+D
Sbjct: 502 MPAEIENYVHRIGRTGRRGKTGVATTFINKNQTETTLLDLKQLLIESKQRLPPILADLDD 561

Query: 553 PMEDVE-AITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           P ED + AI   SGVKGCA+CGGLGHRI  CPK + Q S+ +A +R DYFG GGYRGEI
Sbjct: 562 PQEDDKVAIAQQSGVKGCAFCGGLGHRIEACPKQQLQNSVTLARARSDYFGGGGYRGEI 619

BLAST of MS009388 vs. ExPASy Swiss-Prot
Match: Q9SZB4 (Putative DEAD-box ATP-dependent RNA helicase 43 OS=Arabidopsis thaliana OX=3702 GN=RH43 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 1.1e-256
Identity = 448/587 (76.32%), Postives = 490/587 (83.48%), Query Frame = 0

Query: 11  IEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSLLIKASQMKRD 70
           +E +D YVEYVPV +R A   +K++   GK                              
Sbjct: 1   MEVDDGYVEYVPVEERLAQMKRKVVEEPGKG----------------------------- 60

Query: 71  QPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPPLPIRRMSRKA 130
                           M+EHLSD+K LMSV ELA+GITYTEPL T WKPPL +R+MS K 
Sbjct: 61  ----------------MMEHLSDKKKLMSVGELARGITYTEPLSTWWKPPLHVRKMSTKQ 120

Query: 131 CDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQVQGLPVILSGR 190
            DLIRKQWHI V+G+DIPPPIKNF DM+ P P+L+ LK+KGI+ PTPIQVQGLPV+LSGR
Sbjct: 121 MDLIRKQWHITVNGEDIPPPIKNFMDMKFPSPLLRMLKDKGIMHPTPIQVQGLPVVLSGR 180

Query: 191 DMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEVVEQ 250
           DMIGIAFTGSGKTLVFVLP+I++ALQEE+MMPI +GEGP  L+ICPSRELA+QTY+VVEQ
Sbjct: 181 DMIGIAFTGSGKTLVFVLPMIILALQEEIMMPIAAGEGPIALVICPSRELAKQTYDVVEQ 240

Query: 251 FLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAKKKMNLDNCR 310
           F+  + E GYP LR LLCIGGVDMRSQ++VVKKGVHIVVATPGRLKD+LAKKKM+LD CR
Sbjct: 241 FVASLVEDGYPRLRSLLCIGGVDMRSQLDVVKKGVHIVVATPGRLKDILAKKKMSLDACR 300

Query: 311 YLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTVNVG 370
            LTLDEADRLVDLGFEDDIR VFDHFK+QRQTLLFSATMP KIQ FA SALVKPVTVNVG
Sbjct: 301 LLTLDEADRLVDLGFEDDIRHVFDHFKSQRQTLLFSATMPAKIQIFATSALVKPVTVNVG 360

Query: 371 RAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKGVEA 430
           RAGAANLDVIQEVEYVKQEAKIVYLLECLQKT PPVLIFCENKADVDDIHEYLLLKGVEA
Sbjct: 361 RAGAANLDVIQEVEYVKQEAKIVYLLECLQKTTPPVLIFCENKADVDDIHEYLLLKGVEA 420

Query: 431 VAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENYVHR 490
           VAIHGGKDQE+R+YAIS FKAGKKDVLVATDVASKGLDFPDIQHVINYDMP EIENYVHR
Sbjct: 421 VAIHGGKDQEDRDYAISLFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPGEIENYVHR 480

Query: 491 IGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAITNA 550
           IGRTGRCGKTGIATTFINKNQSE TLLDLKHLLQEAKQRIPPVLAELN PME+ E I NA
Sbjct: 481 IGRTGRCGKTGIATTFINKNQSEITLLDLKHLLQEAKQRIPPVLAELNGPMEETETIANA 540

Query: 551 SGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           SGVKGCAYCGGLGHRI  CPK EHQKS+AI+SSR+D+FGS GYRGE+
Sbjct: 541 SGVKGCAYCGGLGHRILQCPKFEHQKSVAISSSRKDHFGSDGYRGEV 542

BLAST of MS009388 vs. ExPASy Swiss-Prot
Match: Q9UJV9 (Probable ATP-dependent RNA helicase DDX41 OS=Homo sapiens OX=9606 GN=DDX41 PE=1 SV=2)

HSP 1 Score: 699.9 bits (1805), Expect = 2.5e-200
Identity = 359/596 (60.23%), Postives = 458/596 (76.85%), Query Frame = 0

Query: 6   KDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRL-----------A 65
           + E   E++++YV YVP+ +RR L  QK+L R+ K +A EE+ +                
Sbjct: 22  RSEAEDEDDEDYVPYVPLRQRRQLLLQKLLQRRRKGAAEEEQQDSGSEPRGDEDDIPLGP 81

Query: 66  EAKPSLLIKASQMKR--DQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEP 125
           ++  SLL +   +K   +  + S  E+ +++E++++E +++ + LMSV+E+AKGITY +P
Sbjct: 82  QSNVSLLDQHQHLKEKAEARKESAKEKQLKEEEKILESVAEGRALMSVKEMAKGITYDDP 141

Query: 126 LLTGWKPPLPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGI 185
           + T W PP  +  MS +  + +RK++HI+V+GD IPPPIK+FK+M+ P  IL+ LK+KGI
Sbjct: 142 IKTSWTPPRYVLSMSEERHERVRKKYHILVEGDGIPPPIKSFKEMKFPAAILRGLKKKGI 201

Query: 186 VQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGL 245
             PTPIQ+QG+P ILSGRDMIGIAFTGSGKTLVF LP+IM  L++E  +P    EGP+GL
Sbjct: 202 HHPTPIQIQGIPTILSGRDMIGIAFTGSGKTLVFTLPVIMFCLEQEKRLPFSKREGPYGL 261

Query: 246 IICPSRELARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATP 305
           IICPSRELARQT+ ++E +   ++E   P LR  LCIGG+ ++ Q+E ++ GVH++VATP
Sbjct: 262 IICPSRELARQTHGILEYYCRLLQEDSSPLLRCALCIGGMSVKEQMETIRHGVHMMVATP 321

Query: 306 GRLKDMLAKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTK 365
           GRL D+L KK ++LD CRYL LDEADR++D+GFE DIR +F +FK QRQTLLFSATMP K
Sbjct: 322 GRLMDLLQKKMVSLDICRYLALDEADRMIDMGFEGDIRTIFSYFKGQRQTLLFSATMPKK 381

Query: 366 IQNFARSALVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCEN 425
           IQNFA+SALVKPVT+NVGRAGAA+LDVIQEVEYVK+EAK+VYLLECLQKTPPPVLIF E 
Sbjct: 382 IQNFAKSALVKPVTINVGRAGAASLDVIQEVEYVKEEAKMVYLLECLQKTPPPVLIFAEK 441

Query: 426 KADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDI 485
           KADVD IHEYLLLKGVEAVAIHGGKDQEER  AI +F+ GKKDVLVATDVASKGLDFP I
Sbjct: 442 KADVDAIHEYLLLKGVEAVAIHGGKDQEERTKAIEAFREGKKDVLVATDVASKGLDFPAI 501

Query: 486 QHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPP 545
           QHVINYDMP EIENYVHRIGRTGR G TGIATTFINK   E+ L+DLK LL EAKQ++PP
Sbjct: 502 QHVINYDMPEEIENYVHRIGRTGRSGNTGIATTFINKACDESVLMDLKALLLEAKQKVPP 561

Query: 546 VLAELNDPMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIAS-SRRDY 588
           VL  L+      E++ +  G +GCA+CGGLGHRI DCPKLE  ++  +++  R+DY
Sbjct: 562 VLQVLHC---GDESMLDIGGERGCAFCGGLGHRITDCPKLEAMQTKQVSNIGRKDY 614

BLAST of MS009388 vs. ExPASy TrEMBL
Match: A0A6J1D5D5 (RNA helicase OS=Momordica charantia OX=3673 GN=LOC111017080 PE=3 SV=1)

HSP 1 Score: 1171.8 bits (3030), Expect = 0.0e+00
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0

Query: 1   MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL 60
           MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 1   MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL 60

Query: 61  LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120
           LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 61  LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120

Query: 121 LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV 180
           LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV
Sbjct: 121 LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV 180

Query: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240

Query: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA
Sbjct: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300

Query: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360

Query: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420

Query: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM 480
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM 480

Query: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540

Query: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 597

BLAST of MS009388 vs. ExPASy TrEMBL
Match: A0A0A0K6A2 (RNA helicase OS=Cucumis sativus OX=3659 GN=Csa_7G432260 PE=3 SV=1)

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 588/597 (98.49%), Postives = 595/597 (99.66%), Query Frame = 0

Query: 1   MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL 60
           MDVQMKDEK++EEEDEYVEYVPVAKRRA+EAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 121 MDVQMKDEKSLEEEDEYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 180

Query: 61  LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120
           L+KASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 181 LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 240

Query: 121 LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV 180
           LPIRRM +KACDLIRKQWHIIVDGD+IPPPIKNFKDMRLPEP+LKKLKEKGIVQPTPIQV
Sbjct: 241 LPIRRMPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 300

Query: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 301 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 360

Query: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA
Sbjct: 361 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 420

Query: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 421 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 480

Query: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 481 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 540

Query: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM 480
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 541 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 600

Query: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 601 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 660

Query: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 661 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 717

BLAST of MS009388 vs. ExPASy TrEMBL
Match: A0A1S3CDK5 (RNA helicase OS=Cucumis melo OX=3656 GN=LOC103499818 PE=3 SV=1)

HSP 1 Score: 1159.4 bits (2998), Expect = 0.0e+00
Identity = 587/597 (98.32%), Postives = 595/597 (99.66%), Query Frame = 0

Query: 1   MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL 60
           MDVQMKDEK++EEED+YVEYVPVAKRRA+EAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 1   MDVQMKDEKSLEEEDDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 60

Query: 61  LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120
           L+KASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 61  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120

Query: 121 LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV 180
           LPIRRM +KACDLIRKQWHIIVDGD+IPPPIKNFKDMRLPEP+LKKLKEKGIVQPTPIQV
Sbjct: 121 LPIRRMPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 180

Query: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240

Query: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA
Sbjct: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300

Query: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360

Query: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420

Query: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM 480
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 480

Query: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540

Query: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 597

BLAST of MS009388 vs. ExPASy TrEMBL
Match: A0A5A7UYT9 (RNA helicase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G002050 PE=3 SV=1)

HSP 1 Score: 1159.4 bits (2998), Expect = 0.0e+00
Identity = 587/597 (98.32%), Postives = 595/597 (99.66%), Query Frame = 0

Query: 1   MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL 60
           MDVQMKDEK++EEED+YVEYVPVAKRRA+EAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 1   MDVQMKDEKSLEEEDDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 60

Query: 61  LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120
           L+KASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 61  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120

Query: 121 LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV 180
           LPIRRM +KACDLIRKQWHIIVDGD+IPPPIKNFKDMRLPEP+LKKLKEKGIVQPTPIQV
Sbjct: 121 LPIRRMPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 180

Query: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240

Query: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA
Sbjct: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300

Query: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360

Query: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420

Query: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM 480
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 480

Query: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540

Query: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 597

BLAST of MS009388 vs. ExPASy TrEMBL
Match: A0A6J1KU58 (RNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111497657 PE=3 SV=1)

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 586/597 (98.16%), Postives = 594/597 (99.50%), Query Frame = 0

Query: 1   MDVQMKDEKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSL 60
           MDV MKDEK++EEE++YVEYVPVAKRRA+EAQKILMRKGKASALEEELEKSRLAEAKPSL
Sbjct: 2   MDVVMKDEKSMEEEEDYVEYVPVAKRRAMEAQKILMRKGKASALEEELEKSRLAEAKPSL 61

Query: 61  LIKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 120
           L+KASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP
Sbjct: 62  LVKASQMKRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPP 121

Query: 121 LPIRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQV 180
           LPIRRM RKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEP+LKKLKEKGIVQPTPIQV
Sbjct: 122 LPIRRMPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQV 181

Query: 181 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 240
           QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL
Sbjct: 182 QGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSREL 241

Query: 241 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLA 300
           ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIE+VKKGVHIVVATPGRLKDMLA
Sbjct: 242 ARQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLA 301

Query: 301 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 360
           KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA
Sbjct: 302 KKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSA 361

Query: 361 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 420
           LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH
Sbjct: 362 LVKPVTVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIH 421

Query: 421 EYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDM 480
           EYLLLKGVEAVAIHGGKDQEEREYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDM
Sbjct: 422 EYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDM 481

Query: 481 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 540
           PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP
Sbjct: 482 PAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDP 541

Query: 541 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
Sbjct: 542 MEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598

BLAST of MS009388 vs. TAIR 10
Match: AT5G51280.1 (DEAD-box protein abstrakt, putative )

HSP 1 Score: 1041.6 bits (2692), Expect = 2.5e-304
Identity = 522/590 (88.47%), Postives = 557/590 (94.41%), Query Frame = 0

Query: 8   EKNIEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSLLIKASQM 67
           E  +EE D Y+EYV VA+RRA+ AQKIL RKGKAS LEEE +K +LAEAKPSLL++A+Q+
Sbjct: 2   ESIMEEADSYIEYVSVAERRAIAAQKILQRKGKASELEEEADKEKLAEAKPSLLVQATQL 61

Query: 68  KRDQPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPPLPIRRMS 127
           KRD PEVS TEQI+ QEKEM+EHLSD+KTLMSVRELAKGITYTEPLLTGWKPPL IR+MS
Sbjct: 62  KRDVPEVSATEQIILQEKEMMEHLSDKKTLMSVRELAKGITYTEPLLTGWKPPLHIRKMS 121

Query: 128 RKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQVQGLPVIL 187
            K  DLIRKQWHIIV+GDDIPPPIKNFKDM+ P P+L  LKEKGIVQPTPIQVQGLPVIL
Sbjct: 122 SKQRDLIRKQWHIIVNGDDIPPPIKNFKDMKFPRPVLDTLKEKGIVQPTPIQVQGLPVIL 181

Query: 188 SGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEV 247
           +GRDMIGIAFTGSGKTLVFVLP+IMIALQEE+MMPI +GEGP GLI+CPSRELARQTYEV
Sbjct: 182 AGRDMIGIAFTGSGKTLVFVLPMIMIALQEEMMMPIAAGEGPIGLIVCPSRELARQTYEV 241

Query: 248 VEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAKKKMNLD 307
           VEQF+ P+ EAGYP LR LLCIGG+DMRSQ+EVVK+GVHIVVATPGRLKDMLAKKKM+LD
Sbjct: 242 VEQFVAPLVEAGYPPLRSLLCIGGIDMRSQLEVVKRGVHIVVATPGRLKDMLAKKKMSLD 301

Query: 308 NCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTV 367
            CRYLTLDEADRLVDLGFEDDIREVFDHFK+QRQTLLFSATMPTKIQ FARSALVKPVTV
Sbjct: 302 ACRYLTLDEADRLVDLGFEDDIREVFDHFKSQRQTLLFSATMPTKIQIFARSALVKPVTV 361

Query: 368 NVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKG 427
           NVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKT PPVLIFCENKADVDDIHEYLLLKG
Sbjct: 362 NVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTSPPVLIFCENKADVDDIHEYLLLKG 421

Query: 428 VEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENY 487
           VEAVAIHGGKDQE+REYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENY
Sbjct: 422 VEAVAIHGGKDQEDREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENY 481

Query: 488 VHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAI 547
           VHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPME+ E I
Sbjct: 482 VHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEEAETI 541

Query: 548 TNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
            NASGVKGCAYCGGLGHRIRDCPKLEHQKS+AI++SR+DYFGSGGYRGEI
Sbjct: 542 ANASGVKGCAYCGGLGHRIRDCPKLEHQKSVAISNSRKDYFGSGGYRGEI 591

BLAST of MS009388 vs. TAIR 10
Match: AT4G33370.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 887.1 bits (2291), Expect = 7.8e-258
Identity = 448/587 (76.32%), Postives = 490/587 (83.48%), Query Frame = 0

Query: 11  IEEEDEYVEYVPVAKRRALEAQKILMRKGKASALEEELEKSRLAEAKPSLLIKASQMKRD 70
           +E +D YVEYVPV +R A   +K++   GK                              
Sbjct: 1   MEVDDGYVEYVPVEERLAQMKRKVVEEPGKG----------------------------- 60

Query: 71  QPEVSPTEQIVQQEKEMIEHLSDRKTLMSVRELAKGITYTEPLLTGWKPPLPIRRMSRKA 130
                           M+EHLSD+K LMSV ELA+GITYTEPL T WKPPL +R+MS K 
Sbjct: 61  ----------------MMEHLSDKKKLMSVGELARGITYTEPLSTWWKPPLHVRKMSTKQ 120

Query: 131 CDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQVQGLPVILSGR 190
            DLIRKQWHI V+G+DIPPPIKNF DM+ P P+L+ LK+KGI+ PTPIQVQGLPV+LSGR
Sbjct: 121 MDLIRKQWHITVNGEDIPPPIKNFMDMKFPSPLLRMLKDKGIMHPTPIQVQGLPVVLSGR 180

Query: 191 DMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEVVEQ 250
           DMIGIAFTGSGKTLVFVLP+I++ALQEE+MMPI +GEGP  L+ICPSRELA+QTY+VVEQ
Sbjct: 181 DMIGIAFTGSGKTLVFVLPMIILALQEEIMMPIAAGEGPIALVICPSRELAKQTYDVVEQ 240

Query: 251 FLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAKKKMNLDNCR 310
           F+  + E GYP LR LLCIGGVDMRSQ++VVKKGVHIVVATPGRLKD+LAKKKM+LD CR
Sbjct: 241 FVASLVEDGYPRLRSLLCIGGVDMRSQLDVVKKGVHIVVATPGRLKDILAKKKMSLDACR 300

Query: 311 YLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTVNVG 370
            LTLDEADRLVDLGFEDDIR VFDHFK+QRQTLLFSATMP KIQ FA SALVKPVTVNVG
Sbjct: 301 LLTLDEADRLVDLGFEDDIRHVFDHFKSQRQTLLFSATMPAKIQIFATSALVKPVTVNVG 360

Query: 371 RAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLLKGVEA 430
           RAGAANLDVIQEVEYVKQEAKIVYLLECLQKT PPVLIFCENKADVDDIHEYLLLKGVEA
Sbjct: 361 RAGAANLDVIQEVEYVKQEAKIVYLLECLQKTTPPVLIFCENKADVDDIHEYLLLKGVEA 420

Query: 431 VAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENYVHR 490
           VAIHGGKDQE+R+YAIS FKAGKKDVLVATDVASKGLDFPDIQHVINYDMP EIENYVHR
Sbjct: 421 VAIHGGKDQEDRDYAISLFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPGEIENYVHR 480

Query: 491 IGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAITNA 550
           IGRTGRCGKTGIATTFINKNQSE TLLDLKHLLQEAKQRIPPVLAELN PME+ E I NA
Sbjct: 481 IGRTGRCGKTGIATTFINKNQSEITLLDLKHLLQEAKQRIPPVLAELNGPMEETETIANA 540

Query: 551 SGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 598
           SGVKGCAYCGGLGHRI  CPK EHQKS+AI+SSR+D+FGS GYRGE+
Sbjct: 541 SGVKGCAYCGGLGHRILQCPKFEHQKSVAISSSRKDHFGSDGYRGEV 542

BLAST of MS009388 vs. TAIR 10
Match: AT1G55150.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 305.8 bits (782), Expect = 7.4e-83
Identity = 163/406 (40.15%), Postives = 247/406 (60.84%), Query Frame = 0

Query: 135 RKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQVQGLPVILSGRDMIG 194
           RK   I V+G DIP P+K+F+D+  P+ +L+++K+ G  +PTPIQ QG P+ + GRD+IG
Sbjct: 82  RKLREITVEGKDIPKPVKSFRDVGFPDYVLEEVKKAGFTEPTPIQSQGWPMAMKGRDLIG 141

Query: 195 IAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEVVEQFLIP 254
           IA TGSGKTL ++LP I+    + +   +  G+GP  L++ P+RELA Q  +   +F   
Sbjct: 142 IAETGSGKTLSYLLPAIVHVNAQPM---LAHGDGPIVLVLAPTRELAVQIQQEASKF--- 201

Query: 255 MKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAKKKMNLDNCRYLTL 314
                  +++     GGV    Q+  ++KGV IV+ATPGRL DM+     NL    YL L
Sbjct: 202 ---GSSSKIKTTCIYGGVPKGPQVRDLQKGVEIVIATPGRLIDMMESNNTNLRRVTYLVL 261

Query: 315 DEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTVNVGRAG- 374
           DEADR++D+GF+  IR++  H +  RQTL +SAT P +++  ++  L  P  V +G +  
Sbjct: 262 DEADRMLDMGFDPQIRKIVSHIRPDRQTLYWSATWPKEVEQLSKKFLYNPYKVIIGSSDL 321

Query: 375 AANLDVIQEVEYVKQEAKIVYLLECLQ--KTPPPVLIFCENKADVDDIHEYLLLKGVEAV 434
            AN  + Q V+ + +  K   L++ L+       +L+F + K   D I   L + G  A+
Sbjct: 322 KANRAIRQIVDVISESQKYNKLVKLLEDIMDGSRILVFLDTKKGCDQITRQLRMDGWPAL 381

Query: 435 AIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENYVHRI 494
           +IHG K Q ER++ +S F++GK  ++ ATDVA++GLD  D+++VINYD P  +E+YVHRI
Sbjct: 382 SIHGDKSQAERDWVLSEFRSGKSPIMTATDVAARGLDVKDVKYVINYDFPGSLEDYVHRI 441

Query: 495 GRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAEL 538
           GRTGR G  G A TF     +     +L ++LQEA Q++ P LA +
Sbjct: 442 GRTGRAGAKGTAYTFFTVANARFA-KELTNILQEAGQKVSPELASM 477

BLAST of MS009388 vs. TAIR 10
Match: AT2G47330.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 299.7 bits (766), Expect = 5.3e-81
Identity = 177/425 (41.65%), Postives = 246/425 (57.88%), Query Frame = 0

Query: 135 RKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQVQGLPVILSGRDMIG 194
           R++  I V G D+  P+K F+D      I+  +K++   +PT IQ Q LP++LSGRD+IG
Sbjct: 211 RQRLGIRVSGFDVHRPVKTFEDCGFSSQIMSAIKKQAYEKPTAIQCQALPIVLSGRDVIG 270

Query: 195 IAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTYEVVEQFLIP 254
           IA TGSGKT  FVLP+I+  + +  +      EGP G+I  P+RELA Q +   ++F   
Sbjct: 271 IAKTGSGKTAAFVLPMIVHIMDQPELQ---RDEGPIGVICAPTRELAHQIFLEAKKF--- 330

Query: 255 MKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAKKKMNLDNCRYLTL 314
            K  G   LR     GG+    Q + +K G  IVVATPGRL DML  K + +    YL L
Sbjct: 331 SKAYG---LRVSAVYGGMSKHEQFKELKAGCEIVVATPGRLIDMLKMKALTMMRASYLVL 390

Query: 315 DEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPVTVNVGRAGA 374
           DEADR+ DLGFE  +R +    +  RQTLLFSATMP K++  AR  L  P+ V VG  G 
Sbjct: 391 DEADRMFDLGFEPQVRSIVGQIRPDRQTLLFSATMPWKVEKLAREILSDPIRVTVGEVGM 450

Query: 375 ANLDVIQEVEYVKQEA-KIVYLLECL--QKTPPPVLIFCENKADVDDIHEYLLLKGVEAV 434
           AN D+ Q V  +  +A K+ +LLE L        VL+F   KA VD+I   L L   +  
Sbjct: 451 ANEDITQVVNVIPSDAEKLPWLLEKLPGMIDEGDVLVFASKKATVDEIEAQLTLNSFKVA 510

Query: 435 AIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENYVHRI 494
           A+HG KDQ  R   +  FK+G   VL+ATDVA++GLD   ++ V+NYD+  +++ +VHRI
Sbjct: 511 ALHGDKDQASRMETLQKFKSGVHHVLIATDVAARGLDIKSLKTVVNYDIAKDMDMHVHRI 570

Query: 495 GRTGRCG-KTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAITNA 554
           GRTGR G + G+A T + + ++     +L + L  A Q +PP L +L   M+D    +  
Sbjct: 571 GRTGRAGDRDGVAYTLVTQREARFA-GELVNSLVAAGQNVPPELTDL--AMKDGRFKSKR 623

Query: 555 SGVKG 556
            G KG
Sbjct: 631 DGRKG 623

BLAST of MS009388 vs. TAIR 10
Match: AT5G63120.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 1.2e-80
Identity = 172/419 (41.05%), Postives = 248/419 (59.19%), Query Frame = 0

Query: 123 IRRMSRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPILKKLKEKGIVQPTPIQVQG 182
           ++ M+ +   + R +  I V+G D+P P+K F+D   P+ IL+ + + G  +PTPIQ QG
Sbjct: 136 VQAMTEQDVAMYRTERDISVEGRDVPKPMKMFQDANFPDNILEAIAKLGFTEPTPIQAQG 195

Query: 183 LPVILSGRDMIGIAFTGSGKTLVFVLP-LIMIALQEEVMMPIVSGEGPFGLIICPSRELA 242
            P+ L GRD+IGIA TGSGKTL ++LP L+ ++ Q      +   +GP  LI+ P+RELA
Sbjct: 196 WPMALKGRDLIGIAETGSGKTLAYLLPALVHVSAQPR----LGQDDGPIVLILAPTRELA 255

Query: 243 RQTYEVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAK 302
            Q  E   +F +    +G   +R     GG     QI  +++GV IV+ATPGRL DML  
Sbjct: 256 VQIQEESRKFGL---RSG---VRSTCIYGGAPKGPQIRDLRRGVEIVIATPGRLIDMLEC 315

Query: 303 KKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSAL 362
           +  NL    YL LDEADR++D+GFE  IR++    +  RQTLL+SAT P +++  AR  L
Sbjct: 316 QHTNLKRVTYLVLDEADRMLDMGFEPQIRKIVSQIRPDRQTLLWSATWPREVETLARQFL 375

Query: 363 VKPVTVNVGRAG-AANLDVIQEVEYVKQEAKIVYLLECLQK--TPPPVLIFCENKADVDD 422
             P    +G     AN  + Q +E V    K   LL  L++      +LIF E K   D 
Sbjct: 376 RDPYKAIIGSTDLKANQSINQVIEIVPTPEKYNRLLTLLKQLMDGSKILIFVETKRGCDQ 435

Query: 423 IHEYLLLKGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINY 482
           +   L + G  A+AIHG K Q ER+  ++ FK+G+  ++ ATDVA++GLD  DI+ V+NY
Sbjct: 436 VTRQLRMDGWPALAIHGDKTQSERDRVLAEFKSGRSPIMTATDVAARGLDVKDIKCVVNY 495

Query: 483 DMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAEL 538
           D P  +E+Y+HRIGRTGR G  G+A TF   + ++    +L  +LQEA Q +PP L+ L
Sbjct: 496 DFPNTLEDYIHRIGRTGRAGAKGMAFTFFTHDNAKFA-RELVKILQEAGQVVPPTLSAL 543

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148436.10.0e+00100.00DEAD-box ATP-dependent RNA helicase 35 [Momordica charantia][more]
XP_004135864.10.0e+0098.49DEAD-box ATP-dependent RNA helicase 35 [Cucumis sativus] >KAE8646435.1 hypotheti... [more]
XP_008461142.10.0e+0098.32PREDICTED: DEAD-box ATP-dependent RNA helicase 35 [Cucumis melo] >XP_008461143.1... [more]
XP_023004285.10.0e+0098.16DEAD-box ATP-dependent RNA helicase 35 [Cucurbita maxima] >XP_023004286.1 DEAD-b... [more]
KAG6593383.10.0e+0098.16DEAD-box ATP-dependent RNA helicase 35, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
Q9LU463.5e-30388.47DEAD-box ATP-dependent RNA helicase 35 OS=Arabidopsis thaliana OX=3702 GN=RH35 P... [more]
Q0E3X48.1e-28479.74DEAD-box ATP-dependent RNA helicase 35A OS=Oryza sativa subsp. japonica OX=39947... [more]
Q5Z6G59.0e-25975.13DEAD-box ATP-dependent RNA helicase 35B OS=Oryza sativa subsp. japonica OX=39947... [more]
Q9SZB41.1e-25676.32Putative DEAD-box ATP-dependent RNA helicase 43 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9UJV92.5e-20060.23Probable ATP-dependent RNA helicase DDX41 OS=Homo sapiens OX=9606 GN=DDX41 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1D5D50.0e+00100.00RNA helicase OS=Momordica charantia OX=3673 GN=LOC111017080 PE=3 SV=1[more]
A0A0A0K6A20.0e+0098.49RNA helicase OS=Cucumis sativus OX=3659 GN=Csa_7G432260 PE=3 SV=1[more]
A0A1S3CDK50.0e+0098.32RNA helicase OS=Cucumis melo OX=3656 GN=LOC103499818 PE=3 SV=1[more]
A0A5A7UYT90.0e+0098.32RNA helicase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G002050 ... [more]
A0A6J1KU580.0e+0098.16RNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111497657 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G51280.12.5e-30488.47DEAD-box protein abstrakt, putative [more]
AT4G33370.17.8e-25876.32DEA(D/H)-box RNA helicase family protein [more]
AT1G55150.17.4e-8340.15DEA(D/H)-box RNA helicase family protein [more]
AT2G47330.15.3e-8141.65P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G63120.21.2e-8041.05P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 509..529
NoneNo IPR availablePANTHERPTHR47958ATP-DEPENDENT RNA HELICASE DBP3coord: 18..577
NoneNo IPR availablePANTHERPTHR47958:SF79BNAA06G38640D PROTEINcoord: 18..577
NoneNo IPR availableCDDcd18787SF2_C_DEADcoord: 379..507
e-value: 7.60684E-57
score: 185.403
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 417..498
e-value: 8.6E-34
score: 128.3
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 391..498
e-value: 7.9E-31
score: 106.7
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 378..538
score: 23.410553
IPR001878Zinc finger, CCHC-typeSMARTSM00343c2hcfinal6coord: 555..571
e-value: 0.0053
score: 25.3
IPR001878Zinc finger, CCHC-typePFAMPF00098zf-CCHCcoord: 555..571
e-value: 2.0E-4
score: 21.3
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 556..571
score: 9.108787
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 171..382
e-value: 6.6E-57
score: 205.0
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 183..367
score: 30.308105
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 377..539
e-value: 3.2E-56
score: 191.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 121..371
e-value: 2.6E-80
score: 271.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 228..513
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 176..355
e-value: 1.1E-48
score: 165.3
IPR014014RNA helicase, DEAD-box type, Q motifPROSITEPS51195Q_MOTIFcoord: 152..180
score: 10.93678
IPR044113DDX41, DEAD-box helicase domainCDDcd17951DEADc_DDX41coord: 163..368
e-value: 1.99706E-138
score: 399.021
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 555..576

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009388.1MS009388.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
molecular_function GO:0005524 ATP binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003724 RNA helicase activity
molecular_function GO:0008270 zinc ion binding