MS009361 (gene) Bitter gourd (TR) v1

Overview
NameMS009361
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRING-type domain-containing protein
Locationscaffold813: 607956 .. 611671 (+)
RNA-Seq ExpressionMS009361
SyntenyMS009361
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGCGATGGGAAGTGACAATCTTAATTTACAAAATAACCTGTCTGATTCTATTATTCCTGACGATGGGAATGAAGAGTTGAGATGCCTCGGTACAATGAGAACTGATTCTAACTTCAATGGGCTCCATGAAGAGTTCAGTGCGGCTGCTGGATTCCATCATTCCCAATTTCCGGATGCACCAGTCTCCTTCTCTCATCCAAACTTTGCTGGATCCTTCTCTGATTCAATATCATACACTGATATTGATTGGTCGTTCGATGGCTCCCGGTTGGGTCTAAGCTCTCAGAATTGGTCTCACAGCATTTTAACGAATGCCAAAAGTAAGACAAATGCTCCATTCCATGTCAGGACTCCAGAAGTTCCAAGTATTTGTAGAGTCCCAATGAGACATCAGGCAGCTGAAGGCTTACATATAGTTGGAGAAACAAACAATCATAATTTTCAATCCTCCGGCTGTGGGGAAAAGATTGATCTTAATATCACTGCCATTCGCAGTGGATTCCAACCCCATTCTCTTGAAAAGCTTGATTCGGAGACTCTAAAAGCCACCACGGCTCTTAACAAGGCAGATAACATCTCGGGCTTTTCTGATAATTCTGTTGGACTAAGATGTAATAATTTTATTAACTTTACTTTGCACCCATCATCTGTCTTCCAACCATCTATCATAGTTGGTGAGGAGAACCCTCTTCCCACGCCAAGAAATGAAGATGATTTGAATGCTGCAGTAGGACAGAGCAATGGGGATATCCCCAACGCACTTAAACTTGCAGCTTCCTCTAGGACAGCATCTAGTAATAGCCAAATTGGACTATCTTCACGAAGCTGGTCGACTGACTTAGAACCTTGTCCACTTAGAAGGGATCAAAGTCAACAGCCTTCTGAACATCAATCTTATATTGATACCAGGAACTGCTCGTCTCATGAGTGTTCGACTTTTTCTCCTTCCCTTGGGATCTTTGCAACCATCTCTGTACCAGATCGGTCATGTAAGACAGATGGATACTTCAGTTTCATTTACCAACCATACTGCACAAGCAAACATTATTCTTATTAAATGATTATTAATTTTGAATTTGAACATGTTTCTTTGATGCTTGCAGTTGAGCTCCATCAATCTGCTGACCCTTCTCGGCCTCCCCAAAACGTGGGCAATGCTGCCACTTCGGGTACCTCTTTTAGTTCCTTTACTACTCTTCTTGTCTTTAGTTAACACATTTTCATCATATTATATGACTGGAGTAATAACTATAAGCCCAAAAAGTTTCAGGAAATTACAATGCAAGAGAACCTGGGGCTGGTCTCACTCCACATCCTCACAGGGTAACCCCCCTCTCCAACCGAACTTGCTCAATTTCTTCTACATTACTTTCTAACCCACCACGGTTTGCATCTTCCACAACTTCGGCCTCTTAATATTTATGAAGTTTGATCTCAAAACTAATTTGTCGAGAAGCCAATCATCTTTGTTAGATCCCTTAATCTTTTTTACGTTGGATATTCTTAACACGACCAATACTGTGACGAGTCATTTCATGCCCAAATTTCGCAGGTTCTTTCCGTCTCTGCCTCGCCTGATCATATGTCTTCACACGTTTCAATTCAACCTCAGACGCCCAAAATTGATCCAGATCTCCAAACGCAGGAGCAGATTTCCCGACCGATCATTAATGACTACCTTGCACCCTTCCCTTCCAAGTTTGAAGATCTACGAGTTCGTTCTCTCCGACGCTCAAGGAGCTCACACTGTTATATAAAATGTCAAGGTACTTCCAACATATCCCTTTTCCCCATATTTCTTCATATGTTTAGTTTCGTCTGGAAAGTATTATGCATATGCTTTGCGTTCGTACTTGCATATGGTTAGCTCAAAGACAATAATTTTAATTTAAAAAGAAAAACAAGTTGATTACACACATTAGCTCCAATTTACTGTCTCATAATAAAGACAAAGTAAGATCCACCTATCTAGGTGTGTAGCTTTTGTTTTAAAATTTTTATTTCTATTGGAATTTTATTTTTTGTCCGTGGGAGATAGTATTCCTTTTTATGGCACATTCCAAGTAGCATGAACACTAGATTTGTCATTTTGAGCAGAAAGAGAATATTCAAAGCAGAATAGAAACTCTATTTTCAATAGATTATCTGTATAGGCGTCAAACACAGACATGATGTCAAATATGTGTCGGACAAATGATTTAGAGGGTCTTGGTTTTGTTCTGTTCATAGTTGCCAATTTTAAGTTTTGGACACCTGATGGATGCAACCAGATTTCAGACATACTGGGACAGGTATTAAAAGACAACCGACAAAAGTTATAAATAAATAAATTCATACCGACTAAATTTTATATTAAAAACTAGACTAATAACACATGAGAAACCTGCATAGAATAAACTAATCCGATGGACAATTAGAAGAAAAAGCAAAATCAAATTAAAAAATAACATAAAAACAAAACAAATAAACCATGAAGAACTAGGTTTATGAGAGAGAAACCCACAGCATCGATCTAGGTGTGTGGCAGCCAACGACAAAGAAACAGAATGGGGGGAAGAATGGTTTGGCTTGAGATTTTCAGTAGTCATTATTTGGGTATTGACAGTGTGTTTCTTTTTGGTTTACAAGATTTTAGTTCTGGCGGTTTTTACATAGCTAGAGAGAAGAGAGATAGAGAGAAGGGGATGGGGACACTCTTTTCTTGGCGACCACAACAAAGAGAAGGGAGAGTAGAGAGCTCTGTTATATGTCTTATACACAGTAAAGAAGCTGGTTTTGTTCCTACCTTGGAACAAAATTTTCTTATATTAGTTAGTTTCTTCACATAAAGCATGACATGTCTCTGTTCTGTGATTGTGTCGTGTCTAATGTATATATATCGTGAACAGTCATACTCCAACCCTCCTGCATACGTTGAACAGTCGAACTAGAGTATTAGGAATGTTTCTTTCAACACGTCTATACAAAAGAAATTTATTTTTAACCCTGTTGAGTCTAACCTTAAACTTATAATTTATCCTAAATTTATATAAGTGGTGAAACTAAATCTACTAAATACTAAATTGAAAATTTACCAGGAACAACCAAGAAATCACACAAACAAAATATCCGAACTGAAGAAATTAAGCTATGGAATGCTCAAACAACCACGAAAATACTTAAAACAGAACATTAGCGATTTTCATGATAACTTTGGCATGAATAGGAATACTTTGATAAAAGCAAGAGTTAATATGAAAAAAATTCTCCACCTACCAACTACTAGTAATCATGTGACTCATGTCAACATCGTATGAAAATGACAGTCCACCAACATTTAAAAATTATTTTATAAAGACAAAATCATAATGTGAACAAATTGGGATAAATTGAGTACAGTAATCCCGTACCACTATTATGAATTCTTCCTAGAGTTATATATACATTTGTATTGGGTGAGTAAGAGATATTTGTATGTCAACTGCCAAGTGGTGATGGTTAATTTTCTTTGCAGATTTGGACCAAACTCTTGAATTAAGTGGAGAGAGATGTTTCCTATGTAAGAGGGATCTTTCATTCAAACCAGAAGGCTCACTTGTCCTTCCAAAGATCCTACCTGCTGTGGCTGTACTGCCATGTCGCCACGTGTTTCATGACCTCTGTTTGGAACGCATCACTCCTCAAGACCAATCTGAAGATCCTCCCTGTATTCCTTGTGCCGTTGGGGAGACT

mRNA sequence

ATGGACGCGATGGGAAGTGACAATCTTAATTTACAAAATAACCTGTCTGATTCTATTATTCCTGACGATGGGAATGAAGAGTTGAGATGCCTCGGTACAATGAGAACTGATTCTAACTTCAATGGGCTCCATGAAGAGTTCAGTGCGGCTGCTGGATTCCATCATTCCCAATTTCCGGATGCACCAGTCTCCTTCTCTCATCCAAACTTTGCTGGATCCTTCTCTGATTCAATATCATACACTGATATTGATTGGTCGTTCGATGGCTCCCGGTTGGGTCTAAGCTCTCAGAATTGGTCTCACAGCATTTTAACGAATGCCAAAAGTAAGACAAATGCTCCATTCCATGTCAGGACTCCAGAAGTTCCAAGTATTTGTAGAGTCCCAATGAGACATCAGGCAGCTGAAGGCTTACATATAGTTGGAGAAACAAACAATCATAATTTTCAATCCTCCGGCTGTGGGGAAAAGATTGATCTTAATATCACTGCCATTCGCAGTGGATTCCAACCCCATTCTCTTGAAAAGCTTGATTCGGAGACTCTAAAAGCCACCACGGCTCTTAACAAGGCAGATAACATCTCGGGCTTTTCTGATAATTCTGTTGGACTAAGATGTAATAATTTTATTAACTTTACTTTGCACCCATCATCTGTCTTCCAACCATCTATCATAGTTGGTGAGGAGAACCCTCTTCCCACGCCAAGAAATGAAGATGATTTGAATGCTGCAGTAGGACAGAGCAATGGGGATATCCCCAACGCACTTAAACTTGCAGCTTCCTCTAGGACAGCATCTAGTAATAGCCAAATTGGACTATCTTCACGAAGCTGGTCGACTGACTTAGAACCTTGTCCACTTAGAAGGGATCAAAGTCAACAGCCTTCTGAACATCAATCTTATATTGATACCAGGAACTGCTCGTCTCATGAGTGTTCGACTTTTTCTCCTTCCCTTGGGATCTTTGCAACCATCTCTGTACCAGATCGGTCATTTGAGCTCCATCAATCTGCTGACCCTTCTCGGCCTCCCCAAAACGTGGGCAATGCTGCCACTTCGGGTACCTCTTTTAGTTCCTTTACTACTCTTCTTTCATTTCATGCCCAAATTTCGCAGGTTCTTTCCGTCTCTGCCTCGCCTGATCATATGTCTTCACACGTTTCAATTCAACCTCAGACGCCCAAAATTGATCCAGATCTCCAAACGCAGGAGCAGATTTCCCGACCGATCATTAATGACTACCTTGCACCCTTCCCTTCCAAGTTTGAAGATCTACGAGTTCGTTCTCTCCGACGCTCAAGGAGCTCACACTGTTATATAAAATGTCAAGATTTGGACCAAACTCTTGAATTAAGTGGAGAGAGATGTTTCCTATGTAAGAGGGATCTTTCATTCAAACCAGAAGGCTCACTTGTCCTTCCAAAGATCCTACCTGCTGTGGCTGTACTGCCATGTCGCCACGTGTTTCATGACCTCTGTTTGGAACGCATCACTCCTCAAGACCAATCTGAAGATCCTCCCTGTATTCCTTGTGCCGTTGGGGAGACT

Coding sequence (CDS)

ATGGACGCGATGGGAAGTGACAATCTTAATTTACAAAATAACCTGTCTGATTCTATTATTCCTGACGATGGGAATGAAGAGTTGAGATGCCTCGGTACAATGAGAACTGATTCTAACTTCAATGGGCTCCATGAAGAGTTCAGTGCGGCTGCTGGATTCCATCATTCCCAATTTCCGGATGCACCAGTCTCCTTCTCTCATCCAAACTTTGCTGGATCCTTCTCTGATTCAATATCATACACTGATATTGATTGGTCGTTCGATGGCTCCCGGTTGGGTCTAAGCTCTCAGAATTGGTCTCACAGCATTTTAACGAATGCCAAAAGTAAGACAAATGCTCCATTCCATGTCAGGACTCCAGAAGTTCCAAGTATTTGTAGAGTCCCAATGAGACATCAGGCAGCTGAAGGCTTACATATAGTTGGAGAAACAAACAATCATAATTTTCAATCCTCCGGCTGTGGGGAAAAGATTGATCTTAATATCACTGCCATTCGCAGTGGATTCCAACCCCATTCTCTTGAAAAGCTTGATTCGGAGACTCTAAAAGCCACCACGGCTCTTAACAAGGCAGATAACATCTCGGGCTTTTCTGATAATTCTGTTGGACTAAGATGTAATAATTTTATTAACTTTACTTTGCACCCATCATCTGTCTTCCAACCATCTATCATAGTTGGTGAGGAGAACCCTCTTCCCACGCCAAGAAATGAAGATGATTTGAATGCTGCAGTAGGACAGAGCAATGGGGATATCCCCAACGCACTTAAACTTGCAGCTTCCTCTAGGACAGCATCTAGTAATAGCCAAATTGGACTATCTTCACGAAGCTGGTCGACTGACTTAGAACCTTGTCCACTTAGAAGGGATCAAAGTCAACAGCCTTCTGAACATCAATCTTATATTGATACCAGGAACTGCTCGTCTCATGAGTGTTCGACTTTTTCTCCTTCCCTTGGGATCTTTGCAACCATCTCTGTACCAGATCGGTCATTTGAGCTCCATCAATCTGCTGACCCTTCTCGGCCTCCCCAAAACGTGGGCAATGCTGCCACTTCGGGTACCTCTTTTAGTTCCTTTACTACTCTTCTTTCATTTCATGCCCAAATTTCGCAGGTTCTTTCCGTCTCTGCCTCGCCTGATCATATGTCTTCACACGTTTCAATTCAACCTCAGACGCCCAAAATTGATCCAGATCTCCAAACGCAGGAGCAGATTTCCCGACCGATCATTAATGACTACCTTGCACCCTTCCCTTCCAAGTTTGAAGATCTACGAGTTCGTTCTCTCCGACGCTCAAGGAGCTCACACTGTTATATAAAATGTCAAGATTTGGACCAAACTCTTGAATTAAGTGGAGAGAGATGTTTCCTATGTAAGAGGGATCTTTCATTCAAACCAGAAGGCTCACTTGTCCTTCCAAAGATCCTACCTGCTGTGGCTGTACTGCCATGTCGCCACGTGTTTCATGACCTCTGTTTGGAACGCATCACTCCTCAAGACCAATCTGAAGATCCTCCCTGTATTCCTTGTGCCGTTGGGGAGACT

Protein sequence

MDAMGSDNLNLQNNLSDSIIPDDGNEELRCLGTMRTDSNFNGLHEEFSAAAGFHHSQFPDAPVSFSHPNFAGSFSDSISYTDIDWSFDGSRLGLSSQNWSHSILTNAKSKTNAPFHVRTPEVPSICRVPMRHQAAEGLHIVGETNNHNFQSSGCGEKIDLNITAIRSGFQPHSLEKLDSETLKATTALNKADNISGFSDNSVGLRCNNFINFTLHPSSVFQPSIIVGEENPLPTPRNEDDLNAAVGQSNGDIPNALKLAASSRTASSNSQIGLSSRSWSTDLEPCPLRRDQSQQPSEHQSYIDTRNCSSHECSTFSPSLGIFATISVPDRSFELHQSADPSRPPQNVGNAATSGTSFSSFTTLLSFHAQISQVLSVSASPDHMSSHVSIQPQTPKIDPDLQTQEQISRPIINDYLAPFPSKFEDLRVRSLRRSRSSHCYIKCQDLDQTLELSGERCFLCKRDLSFKPEGSLVLPKILPAVAVLPCRHVFHDLCLERITPQDQSEDPPCIPCAVGET
Homology
BLAST of MS009361 vs. NCBI nr
Match: XP_023547724.1 (uncharacterized protein LOC111806582 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 630.9 bits (1626), Expect = 9.4e-177
Identity = 355/563 (63.06%), Postives = 411/563 (73.00%), Query Frame = 0

Query: 1   MDAMGSDNLNLQNNLSDSIIPDDGNEELRCLGTMRTDSNFNGLHEEFSAAAGFHHSQFPD 60
           MD+M  DNLNL++ LS   I DD NEE RCLGT+R+D+NFNGLHEE+SAA+GFHHSQFPD
Sbjct: 4   MDSMEKDNLNLRSKLS-GFISDDENEESRCLGTIRSDANFNGLHEEYSAASGFHHSQFPD 63

Query: 61  APVSFSHPNFAGSFSDSISYTDIDWSFDGSRLGLSSQNWSHSILTNAKSKTNAPFHVRTP 120
             VS SHPN AGSFSDSISYTDIDWSFDGSRLGLSSQ WSHSI  N K+KTNA F +R+P
Sbjct: 64  PSVS-SHPNLAGSFSDSISYTDIDWSFDGSRLGLSSQKWSHSIYANPKNKTNASFSIRSP 123

Query: 121 EVPSICRVPMRHQAAEGLHIVGETNNHNFQSSGCGEKIDLNITAIRSGFQPHSLEKLDSE 180
           E+ SICRVP R   AEG+H V  TNNHNF S+ C ++IDLNITAI S  QPH LE+LD+E
Sbjct: 124 ELASICRVPARTHEAEGIHRV--TNNHNFHSAECEDQIDLNITAICSALQPHGLEELDAE 183

Query: 181 TL------KATTALNKADNISGFSD-------------NSVGLRCNNFINFTLHPSSVFQ 240
            L      KATTA+NKAD ISGF D             NSVG+RC+NFINF+LH SSVFQ
Sbjct: 184 ALDVGCQTKATTAVNKADKISGFKDNSGDIPNLTTFPQNSVGVRCSNFINFSLHQSSVFQ 243

Query: 241 PSIIVGEENPLPTPRNEDD--LNAAVGQSNGDIPNALKLAASSRTASSNSQIGLSSRSWS 300
           PSIIVG +NP PTP   DD  L  AV QS+ DI N+LK A    T S NS+I LSS+ W 
Sbjct: 244 PSIIVGGDNPFPTPSFTDDSGLTPAVVQSDEDILNSLKNAPPPTTESVNSKIELSSQCWF 303

Query: 301 TDLEPCPLRRDQSQQPSEHQSYIDTRNCSSHECSTFSPSLGIFATISVPDRSFELHQSAD 360
           TDLE C LRRDQSQ+PSE +S  D RN +S E  TF+PSLGI ATISVPD+S EL QSA 
Sbjct: 304 TDLESCLLRRDQSQRPSEQKSPPDVRNFAS-EFPTFAPSLGISATISVPDQSVELVQSAG 363

Query: 361 PSRPPQNVGNAATSG--------------------------TSFSSFTTLLSFHAQISQV 420
           PS+PPQNVGNA  SG                          +S  S  T +SF A ++QV
Sbjct: 364 PSQPPQNVGNALASGNQDAREFGTVEDSGIAKSNPNPPVFTSSPVSANTDISFQAHLAQV 423

Query: 421 LSVSASPDHMSSHVSIQPQTPKIDPDLQTQEQISRPIINDYLAPFPSKFEDLRVRSLRRS 480
           LSVS SPD ++SHV IQPQ  +IDP+     Q+ +PII++ +    SKFEDLR RS RRS
Sbjct: 424 LSVSVSPD-ITSHVVIQPQKTEIDPN-----QVYQPIISESVPLVSSKFEDLRDRSFRRS 483

Query: 481 RSSHCYIKCQDLDQTLELSGERCFLCKRDLSFKPEGSLVLPKILPAVAVLPCRHVFHDLC 517
           R+S CYIKC+DLDQT +LSGERCF+CKRDLSFKPEGSL+LPKI P VAVLPC HVFH LC
Sbjct: 484 RTSRCYIKCRDLDQTPKLSGERCFICKRDLSFKPEGSLILPKIPPTVAVLPCSHVFHGLC 543

BLAST of MS009361 vs. NCBI nr
Match: XP_022991862.1 (uncharacterized protein LOC111488380 isoform X2 [Cucurbita maxima])

HSP 1 Score: 630.9 bits (1626), Expect = 9.4e-177
Identity = 355/563 (63.06%), Postives = 410/563 (72.82%), Query Frame = 0

Query: 1   MDAMGSDNLNLQNNLSDSIIPDDGNEELRCLGTMRTDSNFNGLHEEFSAAAGFHHSQFPD 60
           MD+M  DNLNL+N LS   I DD NEE RCLGT+R+D+NFNGLHEE+SAA+G HHSQFPD
Sbjct: 4   MDSMEKDNLNLRNKLS-GFISDDENEESRCLGTIRSDANFNGLHEEYSAASGLHHSQFPD 63

Query: 61  APVSFSHPNFAGSFSDSISYTDIDWSFDGSRLGLSSQNWSHSILTNAKSKTNAPFHVRTP 120
             VS SHPN AGSFSDSISYTDIDWSFDGSRLGLSSQ WS+SI  N K+KTNA F +R+P
Sbjct: 64  PSVS-SHPNLAGSFSDSISYTDIDWSFDGSRLGLSSQKWSNSIYANPKNKTNASFRIRSP 123

Query: 121 EVPSICRVPMRHQAAEGLHIVGETNNHNFQSSGCGEKIDLNITAIRSGFQPHSLEKLDSE 180
           E+ SICRVP R   AEG+H V  TNNHNF S+ C ++IDLNITAI S  QPH LE+LD+E
Sbjct: 124 ELASICRVPARSHEAEGIHRV--TNNHNFHSAECEDQIDLNITAICSALQPHGLEELDAE 183

Query: 181 TL------KATTALNKADNISGFSD-------------NSVGLRCNNFINFTLHPSSVFQ 240
            L      KATTALNKAD ISGF D             NSVGLRC+NFINF+LH SSVFQ
Sbjct: 184 ALDVGCQTKATTALNKADKISGFKDNSGDIPNLTTFPQNSVGLRCSNFINFSLHQSSVFQ 243

Query: 241 PSIIVGEENPLPTPRNEDD--LNAAVGQSNGDIPNALKLAASSRTASSNSQIGLSSRSWS 300
           PSIIVG +NP PTP   DD  L  AV Q + DI N+LK A    T S NS+I LSS+ W 
Sbjct: 244 PSIIVGGDNPFPTPSFTDDSGLTPAVVQGDEDILNSLKNAPPLTTESVNSKIELSSQCWF 303

Query: 301 TDLEPCPLRRDQSQQPSEHQSYIDTRNCSSHECSTFSPSLGIFATISVPDRSFELHQSAD 360
           TDLE C LRRDQSQ+PSE +S+ D RN +S E  TF+PSLGI ATISVPD+S EL QSA 
Sbjct: 304 TDLESCLLRRDQSQRPSEQKSHPDVRNFTS-EFPTFAPSLGISATISVPDQSVELVQSAG 363

Query: 361 PSRPPQNVGNAATSG--------------------------TSFSSFTTLLSFHAQISQV 420
           PS+PPQNVGNA  SG                          +S  S  T +SF A ++QV
Sbjct: 364 PSQPPQNVGNALASGNQDAREFGTVEDSGIAKSNPHPPVFTSSPVSANTDISFQAHLAQV 423

Query: 421 LSVSASPDHMSSHVSIQPQTPKIDPDLQTQEQISRPIINDYLAPFPSKFEDLRVRSLRRS 480
           LSVS SPD ++SHV IQPQ  +IDP+     Q+ +PII++ +    SKFEDLR RS RRS
Sbjct: 424 LSVSVSPD-ITSHVVIQPQKTEIDPN-----QVYQPIISESVPLVSSKFEDLRDRSFRRS 483

Query: 481 RSSHCYIKCQDLDQTLELSGERCFLCKRDLSFKPEGSLVLPKILPAVAVLPCRHVFHDLC 517
           R+S CYIKC+DLDQT +LSGERCF+CKRDLSFKPEGSLVLPKI P VA+LPC HVFH LC
Sbjct: 484 RTSRCYIKCRDLDQTPKLSGERCFICKRDLSFKPEGSLVLPKIPPTVAILPCSHVFHGLC 543

BLAST of MS009361 vs. NCBI nr
Match: KAG7014196.1 (hypothetical protein SDJN02_24371, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 629.8 bits (1623), Expect = 2.1e-176
Identity = 353/563 (62.70%), Postives = 410/563 (72.82%), Query Frame = 0

Query: 1   MDAMGSDNLNLQNNLSDSIIPDDGNEELRCLGTMRTDSNFNGLHEEFSAAAGFHHSQFPD 60
           MD++  DNLNL+N  S   I DD NEE RCLGT+R+D+NFNGLHEE+SAA+GFHHSQFPD
Sbjct: 1   MDSIEKDNLNLRNKRS-GFISDDENEESRCLGTIRSDANFNGLHEEYSAASGFHHSQFPD 60

Query: 61  APVSFSHPNFAGSFSDSISYTDIDWSFDGSRLGLSSQNWSHSILTNAKSKTNAPFHVRTP 120
             VS SHPN AGSFSDSISYTDIDWSFDGSRLGLSSQ WSHSI  N K+KTNA F +R+P
Sbjct: 61  PSVS-SHPNLAGSFSDSISYTDIDWSFDGSRLGLSSQKWSHSIYANPKNKTNASFRIRSP 120

Query: 121 EVPSICRVPMRHQAAEGLHIVGETNNHNFQSSGCGEKIDLNITAIRSGFQPHSLEKLDSE 180
           E+ SICRVP R   AEG+H V  TNNHNF S+ C ++IDLNITAI S  QPH LE+LD+E
Sbjct: 121 ELASICRVPARSHEAEGIHRV--TNNHNFHSAECEDQIDLNITAICSALQPHGLEELDAE 180

Query: 181 TL------KATTALNKADNISGFSD-------------NSVGLRCNNFINFTLHPSSVFQ 240
            L      KATTA+NKAD ISGF D             NSVG+RC+NFINF+LH SSVFQ
Sbjct: 181 ALDVGCQTKATTAVNKADKISGFKDNSGDIPNLTTFPQNSVGVRCSNFINFSLHQSSVFQ 240

Query: 241 PSIIVGEENPLPTPRNEDD--LNAAVGQSNGDIPNALKLAASSRTASSNSQIGLSSRSWS 300
           PSIIVG +NP PTP   DD  L  AV Q + DI N+LK A    T S NS+I LSS+ W 
Sbjct: 241 PSIIVGGDNPFPTPSFTDDSGLTPAVVQGDEDILNSLKNAPPPTTESVNSKIELSSQCWF 300

Query: 301 TDLEPCPLRRDQSQQPSEHQSYIDTRNCSSHECSTFSPSLGIFATISVPDRSFELHQSAD 360
           TDLE C LRRDQSQ+PSE +S+ D RN +S E  TF+PSLGI ATISVPD+S EL QSA 
Sbjct: 301 TDLESCLLRRDQSQRPSEQKSHPDVRNFAS-EFPTFAPSLGISATISVPDQSVELVQSAG 360

Query: 361 PSRPPQNVGNAATSG--------------------------TSFSSFTTLLSFHAQISQV 420
           PS+PPQNVGNA  SG                          +S  S  T +SF A ++QV
Sbjct: 361 PSQPPQNVGNALASGNQDAKEFGTVEDSGIAKSNPNPPVFASSPVSANTDISFKAHLAQV 420

Query: 421 LSVSASPDHMSSHVSIQPQTPKIDPDLQTQEQISRPIINDYLAPFPSKFEDLRVRSLRRS 480
           LSVS SPD ++SHV IQPQ  +IDP+     Q+ +PII++ +    SKFEDLR RS RRS
Sbjct: 421 LSVSVSPD-ITSHVVIQPQKTEIDPN-----QVYQPIISESVPLVSSKFEDLRDRSFRRS 480

Query: 481 RSSHCYIKCQDLDQTLELSGERCFLCKRDLSFKPEGSLVLPKILPAVAVLPCRHVFHDLC 517
           R+S CYIKC+DLDQT +LSGERCF+CKRDLSFKPEGSL+LPKI P VAVLPC HVFH LC
Sbjct: 481 RTSRCYIKCRDLDQTPKLSGERCFICKRDLSFKPEGSLILPKIPPTVAVLPCSHVFHGLC 540

BLAST of MS009361 vs. NCBI nr
Match: XP_022991860.1 (uncharacterized protein LOC111488380 isoform X1 [Cucurbita maxima])

HSP 1 Score: 627.5 bits (1617), Expect = 1.0e-175
Identity = 356/565 (63.01%), Postives = 410/565 (72.57%), Query Frame = 0

Query: 1   MDAMGSDNLNLQNNLSDSIIPDDGNEELRCLGTMRTDSNFNGLHEEFSAAAGFHHSQFPD 60
           MD+M  DNLNL+N LS   I DD NEE RCLGT+R+D+NFNGLHEE+SAA+G HHSQFPD
Sbjct: 4   MDSMEKDNLNLRNKLS-GFISDDENEESRCLGTIRSDANFNGLHEEYSAASGLHHSQFPD 63

Query: 61  APVSFSHPNFAGSFSDSISYTDIDWSFDGSRLGLSSQNWSHSILTNAKSKTNAPFHVRTP 120
             VS SHPN AGSFSDSISYTDIDWSFDGSRLGLSSQ WS+SI  N K+KTNA F +R+P
Sbjct: 64  PSVS-SHPNLAGSFSDSISYTDIDWSFDGSRLGLSSQKWSNSIYANPKNKTNASFRIRSP 123

Query: 121 EVPSICRVPMRHQAAEGLHIVGETNNHNFQSSGCGEKIDLNITAIRSGFQPHSLEKLDSE 180
           E+ SICRVP R   AEG+H V  TNNHNF S+ C ++IDLNITAI S  QPH LE+LD+E
Sbjct: 124 ELASICRVPARSHEAEGIHRV--TNNHNFHSAECEDQIDLNITAICSALQPHGLEELDAE 183

Query: 181 TL------KATTALNKADNISGFSD-------------NSVGLRCNNFINFTLHPSSVFQ 240
            L      KATTALNKAD ISGF D             NSVGLRC+NFINF+LH SSVFQ
Sbjct: 184 ALDVGCQTKATTALNKADKISGFKDNSGDIPNLTTFPQNSVGLRCSNFINFSLHQSSVFQ 243

Query: 241 PSIIVGEENPLPTPRNEDD--LNAAVGQSNGDIPNALKLAASSRTASSNSQIGLSSRSWS 300
           PSIIVG +NP PTP   DD  L  AV Q + DI N+LK A    T S NS+I LSS+ W 
Sbjct: 244 PSIIVGGDNPFPTPSFTDDSGLTPAVVQGDEDILNSLKNAPPLTTESVNSKIELSSQCWF 303

Query: 301 TDLEPCPLRRDQSQQPSEHQSYIDTRNCSSHECSTFSPSLGIFATISVPDRSFELHQSAD 360
           TDLE C LRRDQSQ+PSE +S+ D RN +S E  TF+PSLGI ATISVPD+S EL QSA 
Sbjct: 304 TDLESCLLRRDQSQRPSEQKSHPDVRNFTS-EFPTFAPSLGISATISVPDQSVELVQSAG 363

Query: 361 PSRPPQNVGNAATS----------GT------------------SFSSFTTLLSFHAQIS 420
           PS+PPQNVGNA  S          GT                  S  S  T +SF A ++
Sbjct: 364 PSQPPQNVGNALASVSGNQDAREFGTVEDSGIAKSNPHPPVFTSSPVSANTDISFQAHLA 423

Query: 421 QVLSVSASPDHMSSHVSIQPQTPKIDPDLQTQEQISRPIINDYLAPFPSKFEDLRVRSLR 480
           QVLSVS SPD ++SHV IQPQ  +IDP+     Q+ +PII++ +    SKFEDLR RS R
Sbjct: 424 QVLSVSVSPD-ITSHVVIQPQKTEIDPN-----QVYQPIISESVPLVSSKFEDLRDRSFR 483

Query: 481 RSRSSHCYIKCQDLDQTLELSGERCFLCKRDLSFKPEGSLVLPKILPAVAVLPCRHVFHD 517
           RSR+S CYIKC+DLDQT +LSGERCF+CKRDLSFKPEGSLVLPKI P VA+LPC HVFH 
Sbjct: 484 RSRTSRCYIKCRDLDQTPKLSGERCFICKRDLSFKPEGSLVLPKIPPTVAILPCSHVFHG 543

BLAST of MS009361 vs. NCBI nr
Match: XP_023547723.1 (uncharacterized protein LOC111806582 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627.5 bits (1617), Expect = 1.0e-175
Identity = 356/565 (63.01%), Postives = 411/565 (72.74%), Query Frame = 0

Query: 1   MDAMGSDNLNLQNNLSDSIIPDDGNEELRCLGTMRTDSNFNGLHEEFSAAAGFHHSQFPD 60
           MD+M  DNLNL++ LS   I DD NEE RCLGT+R+D+NFNGLHEE+SAA+GFHHSQFPD
Sbjct: 4   MDSMEKDNLNLRSKLS-GFISDDENEESRCLGTIRSDANFNGLHEEYSAASGFHHSQFPD 63

Query: 61  APVSFSHPNFAGSFSDSISYTDIDWSFDGSRLGLSSQNWSHSILTNAKSKTNAPFHVRTP 120
             VS SHPN AGSFSDSISYTDIDWSFDGSRLGLSSQ WSHSI  N K+KTNA F +R+P
Sbjct: 64  PSVS-SHPNLAGSFSDSISYTDIDWSFDGSRLGLSSQKWSHSIYANPKNKTNASFSIRSP 123

Query: 121 EVPSICRVPMRHQAAEGLHIVGETNNHNFQSSGCGEKIDLNITAIRSGFQPHSLEKLDSE 180
           E+ SICRVP R   AEG+H V  TNNHNF S+ C ++IDLNITAI S  QPH LE+LD+E
Sbjct: 124 ELASICRVPARTHEAEGIHRV--TNNHNFHSAECEDQIDLNITAICSALQPHGLEELDAE 183

Query: 181 TL------KATTALNKADNISGFSD-------------NSVGLRCNNFINFTLHPSSVFQ 240
            L      KATTA+NKAD ISGF D             NSVG+RC+NFINF+LH SSVFQ
Sbjct: 184 ALDVGCQTKATTAVNKADKISGFKDNSGDIPNLTTFPQNSVGVRCSNFINFSLHQSSVFQ 243

Query: 241 PSIIVGEENPLPTPRNEDD--LNAAVGQSNGDIPNALKLAASSRTASSNSQIGLSSRSWS 300
           PSIIVG +NP PTP   DD  L  AV QS+ DI N+LK A    T S NS+I LSS+ W 
Sbjct: 244 PSIIVGGDNPFPTPSFTDDSGLTPAVVQSDEDILNSLKNAPPPTTESVNSKIELSSQCWF 303

Query: 301 TDLEPCPLRRDQSQQPSEHQSYIDTRNCSSHECSTFSPSLGIFATISVPDRSFELHQSAD 360
           TDLE C LRRDQSQ+PSE +S  D RN +S E  TF+PSLGI ATISVPD+S EL QSA 
Sbjct: 304 TDLESCLLRRDQSQRPSEQKSPPDVRNFAS-EFPTFAPSLGISATISVPDQSVELVQSAG 363

Query: 361 PSRPPQNVGNAATS----------GT------------------SFSSFTTLLSFHAQIS 420
           PS+PPQNVGNA  S          GT                  S  S  T +SF A ++
Sbjct: 364 PSQPPQNVGNALASVSGNQDAREFGTVEDSGIAKSNPNPPVFTSSPVSANTDISFQAHLA 423

Query: 421 QVLSVSASPDHMSSHVSIQPQTPKIDPDLQTQEQISRPIINDYLAPFPSKFEDLRVRSLR 480
           QVLSVS SPD ++SHV IQPQ  +IDP+     Q+ +PII++ +    SKFEDLR RS R
Sbjct: 424 QVLSVSVSPD-ITSHVVIQPQKTEIDPN-----QVYQPIISESVPLVSSKFEDLRDRSFR 483

Query: 481 RSRSSHCYIKCQDLDQTLELSGERCFLCKRDLSFKPEGSLVLPKILPAVAVLPCRHVFHD 517
           RSR+S CYIKC+DLDQT +LSGERCF+CKRDLSFKPEGSL+LPKI P VAVLPC HVFH 
Sbjct: 484 RSRTSRCYIKCRDLDQTPKLSGERCFICKRDLSFKPEGSLILPKIPPTVAVLPCSHVFHG 543

BLAST of MS009361 vs. ExPASy TrEMBL
Match: A0A6J1JN28 (uncharacterized protein LOC111488380 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488380 PE=4 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 4.5e-177
Identity = 355/563 (63.06%), Postives = 410/563 (72.82%), Query Frame = 0

Query: 1   MDAMGSDNLNLQNNLSDSIIPDDGNEELRCLGTMRTDSNFNGLHEEFSAAAGFHHSQFPD 60
           MD+M  DNLNL+N LS   I DD NEE RCLGT+R+D+NFNGLHEE+SAA+G HHSQFPD
Sbjct: 4   MDSMEKDNLNLRNKLS-GFISDDENEESRCLGTIRSDANFNGLHEEYSAASGLHHSQFPD 63

Query: 61  APVSFSHPNFAGSFSDSISYTDIDWSFDGSRLGLSSQNWSHSILTNAKSKTNAPFHVRTP 120
             VS SHPN AGSFSDSISYTDIDWSFDGSRLGLSSQ WS+SI  N K+KTNA F +R+P
Sbjct: 64  PSVS-SHPNLAGSFSDSISYTDIDWSFDGSRLGLSSQKWSNSIYANPKNKTNASFRIRSP 123

Query: 121 EVPSICRVPMRHQAAEGLHIVGETNNHNFQSSGCGEKIDLNITAIRSGFQPHSLEKLDSE 180
           E+ SICRVP R   AEG+H V  TNNHNF S+ C ++IDLNITAI S  QPH LE+LD+E
Sbjct: 124 ELASICRVPARSHEAEGIHRV--TNNHNFHSAECEDQIDLNITAICSALQPHGLEELDAE 183

Query: 181 TL------KATTALNKADNISGFSD-------------NSVGLRCNNFINFTLHPSSVFQ 240
            L      KATTALNKAD ISGF D             NSVGLRC+NFINF+LH SSVFQ
Sbjct: 184 ALDVGCQTKATTALNKADKISGFKDNSGDIPNLTTFPQNSVGLRCSNFINFSLHQSSVFQ 243

Query: 241 PSIIVGEENPLPTPRNEDD--LNAAVGQSNGDIPNALKLAASSRTASSNSQIGLSSRSWS 300
           PSIIVG +NP PTP   DD  L  AV Q + DI N+LK A    T S NS+I LSS+ W 
Sbjct: 244 PSIIVGGDNPFPTPSFTDDSGLTPAVVQGDEDILNSLKNAPPLTTESVNSKIELSSQCWF 303

Query: 301 TDLEPCPLRRDQSQQPSEHQSYIDTRNCSSHECSTFSPSLGIFATISVPDRSFELHQSAD 360
           TDLE C LRRDQSQ+PSE +S+ D RN +S E  TF+PSLGI ATISVPD+S EL QSA 
Sbjct: 304 TDLESCLLRRDQSQRPSEQKSHPDVRNFTS-EFPTFAPSLGISATISVPDQSVELVQSAG 363

Query: 361 PSRPPQNVGNAATSG--------------------------TSFSSFTTLLSFHAQISQV 420
           PS+PPQNVGNA  SG                          +S  S  T +SF A ++QV
Sbjct: 364 PSQPPQNVGNALASGNQDAREFGTVEDSGIAKSNPHPPVFTSSPVSANTDISFQAHLAQV 423

Query: 421 LSVSASPDHMSSHVSIQPQTPKIDPDLQTQEQISRPIINDYLAPFPSKFEDLRVRSLRRS 480
           LSVS SPD ++SHV IQPQ  +IDP+     Q+ +PII++ +    SKFEDLR RS RRS
Sbjct: 424 LSVSVSPD-ITSHVVIQPQKTEIDPN-----QVYQPIISESVPLVSSKFEDLRDRSFRRS 483

Query: 481 RSSHCYIKCQDLDQTLELSGERCFLCKRDLSFKPEGSLVLPKILPAVAVLPCRHVFHDLC 517
           R+S CYIKC+DLDQT +LSGERCF+CKRDLSFKPEGSLVLPKI P VA+LPC HVFH LC
Sbjct: 484 RTSRCYIKCRDLDQTPKLSGERCFICKRDLSFKPEGSLVLPKIPPTVAILPCSHVFHGLC 543

BLAST of MS009361 vs. ExPASy TrEMBL
Match: A0A6J1JU43 (uncharacterized protein LOC111488380 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488380 PE=4 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 5.0e-176
Identity = 356/565 (63.01%), Postives = 410/565 (72.57%), Query Frame = 0

Query: 1   MDAMGSDNLNLQNNLSDSIIPDDGNEELRCLGTMRTDSNFNGLHEEFSAAAGFHHSQFPD 60
           MD+M  DNLNL+N LS   I DD NEE RCLGT+R+D+NFNGLHEE+SAA+G HHSQFPD
Sbjct: 4   MDSMEKDNLNLRNKLS-GFISDDENEESRCLGTIRSDANFNGLHEEYSAASGLHHSQFPD 63

Query: 61  APVSFSHPNFAGSFSDSISYTDIDWSFDGSRLGLSSQNWSHSILTNAKSKTNAPFHVRTP 120
             VS SHPN AGSFSDSISYTDIDWSFDGSRLGLSSQ WS+SI  N K+KTNA F +R+P
Sbjct: 64  PSVS-SHPNLAGSFSDSISYTDIDWSFDGSRLGLSSQKWSNSIYANPKNKTNASFRIRSP 123

Query: 121 EVPSICRVPMRHQAAEGLHIVGETNNHNFQSSGCGEKIDLNITAIRSGFQPHSLEKLDSE 180
           E+ SICRVP R   AEG+H V  TNNHNF S+ C ++IDLNITAI S  QPH LE+LD+E
Sbjct: 124 ELASICRVPARSHEAEGIHRV--TNNHNFHSAECEDQIDLNITAICSALQPHGLEELDAE 183

Query: 181 TL------KATTALNKADNISGFSD-------------NSVGLRCNNFINFTLHPSSVFQ 240
            L      KATTALNKAD ISGF D             NSVGLRC+NFINF+LH SSVFQ
Sbjct: 184 ALDVGCQTKATTALNKADKISGFKDNSGDIPNLTTFPQNSVGLRCSNFINFSLHQSSVFQ 243

Query: 241 PSIIVGEENPLPTPRNEDD--LNAAVGQSNGDIPNALKLAASSRTASSNSQIGLSSRSWS 300
           PSIIVG +NP PTP   DD  L  AV Q + DI N+LK A    T S NS+I LSS+ W 
Sbjct: 244 PSIIVGGDNPFPTPSFTDDSGLTPAVVQGDEDILNSLKNAPPLTTESVNSKIELSSQCWF 303

Query: 301 TDLEPCPLRRDQSQQPSEHQSYIDTRNCSSHECSTFSPSLGIFATISVPDRSFELHQSAD 360
           TDLE C LRRDQSQ+PSE +S+ D RN +S E  TF+PSLGI ATISVPD+S EL QSA 
Sbjct: 304 TDLESCLLRRDQSQRPSEQKSHPDVRNFTS-EFPTFAPSLGISATISVPDQSVELVQSAG 363

Query: 361 PSRPPQNVGNAATS----------GT------------------SFSSFTTLLSFHAQIS 420
           PS+PPQNVGNA  S          GT                  S  S  T +SF A ++
Sbjct: 364 PSQPPQNVGNALASVSGNQDAREFGTVEDSGIAKSNPHPPVFTSSPVSANTDISFQAHLA 423

Query: 421 QVLSVSASPDHMSSHVSIQPQTPKIDPDLQTQEQISRPIINDYLAPFPSKFEDLRVRSLR 480
           QVLSVS SPD ++SHV IQPQ  +IDP+     Q+ +PII++ +    SKFEDLR RS R
Sbjct: 424 QVLSVSVSPD-ITSHVVIQPQKTEIDPN-----QVYQPIISESVPLVSSKFEDLRDRSFR 483

Query: 481 RSRSSHCYIKCQDLDQTLELSGERCFLCKRDLSFKPEGSLVLPKILPAVAVLPCRHVFHD 517
           RSR+S CYIKC+DLDQT +LSGERCF+CKRDLSFKPEGSLVLPKI P VA+LPC HVFH 
Sbjct: 484 RSRTSRCYIKCRDLDQTPKLSGERCFICKRDLSFKPEGSLVLPKIPPTVAILPCSHVFHG 543

BLAST of MS009361 vs. ExPASy TrEMBL
Match: A0A6J1GPA7 (uncharacterized protein LOC111456279 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456279 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 3.3e-175
Identity = 354/563 (62.88%), Postives = 409/563 (72.65%), Query Frame = 0

Query: 1   MDAMGSDNLNLQNNLSDSIIPDDGNEELRCLGTMRTDSNFNGLHEEFSAAAGFHHSQFPD 60
           MD++  DNLNL+N LS   I DD NEE RCLGT+R+D+NFNGLHEE+SAA+ FHHSQFPD
Sbjct: 4   MDSIEKDNLNLRNKLS-GFISDDENEESRCLGTIRSDANFNGLHEEYSAASRFHHSQFPD 63

Query: 61  APVSFSHPNFAGSFSDSISYTDIDWSFDGSRLGLSSQNWSHSILTNAKSKTNAPFHVRTP 120
             VS SHPN AGSFSDSISYTDIDWSFDGSRLGLSSQ WSHSI  N K+KTNA F +R+P
Sbjct: 64  PSVS-SHPNLAGSFSDSISYTDIDWSFDGSRLGLSSQKWSHSIYANPKNKTNASFRIRSP 123

Query: 121 EVPSICRVPMRHQAAEGLHIVGETNNHNFQSSGCGEKIDLNITAIRSGFQPHSLEKLDSE 180
           E+ SICRVP R   AEG+H V  TNNHNF S+ C ++IDLNITAI S  QPH LE+LD E
Sbjct: 124 ELASICRVPARSHEAEGIHRV--TNNHNFHSAECEDQIDLNITAICSALQPHGLEELDDE 183

Query: 181 TL------KATTALNKADNISGFSD-------------NSVGLRCNNFINFTLHPSSVFQ 240
            L      KATTALNKAD ISGF D             NSVG+RC+NFINF+LH SSVFQ
Sbjct: 184 ALDVGCQTKATTALNKADKISGFKDNSGDIPNLTTFPQNSVGVRCSNFINFSLHQSSVFQ 243

Query: 241 PSIIVGEENPLPTPRNEDD--LNAAVGQSNGDIPNALKLAASSRTASSNSQIGLSSRSWS 300
           PSIIVG +NP PTP   DD  L  AV Q + DI N+LK  A   T S NS+I LSS+ W 
Sbjct: 244 PSIIVGGDNPFPTPSFTDDSGLTPAVVQGDEDILNSLK-NAPPPTESVNSKIELSSQCWF 303

Query: 301 TDLEPCPLRRDQSQQPSEHQSYIDTRNCSSHECSTFSPSLGIFATISVPDRSFELHQSAD 360
           TDLE C LR DQSQ+PSE +S+ D RN +S E  TF+PSLGI ATISVPD+S EL QSA 
Sbjct: 304 TDLESCLLRSDQSQRPSEQKSHPDVRNFAS-EFPTFAPSLGISATISVPDQSVELVQSAG 363

Query: 361 PSRPPQNVGNAATSG--------------------------TSFSSFTTLLSFHAQISQV 420
           PS+PPQNVGNA  SG                          +S  S  T +SF A ++QV
Sbjct: 364 PSQPPQNVGNALASGNQDAREFGTVEDSGIAKSNPNPPVFTSSPVSANTDISFQAHLAQV 423

Query: 421 LSVSASPDHMSSHVSIQPQTPKIDPDLQTQEQISRPIINDYLAPFPSKFEDLRVRSLRRS 480
           LSVSASPD ++SHV IQPQ  +IDP+     Q+ +PII++ +    SKFEDLR RS RRS
Sbjct: 424 LSVSASPD-ITSHVVIQPQKTEIDPN-----QVYQPIISESVPLVSSKFEDLRDRSFRRS 483

Query: 481 RSSHCYIKCQDLDQTLELSGERCFLCKRDLSFKPEGSLVLPKILPAVAVLPCRHVFHDLC 517
           R+S CYIKC+DLDQT +LSGERCF+CKRDLSFKPEGSL+LPKI P VAVLPC HVFH LC
Sbjct: 484 RTSRCYIKCRDLDQTPKLSGERCFICKRDLSFKPEGSLILPKIPPTVAVLPCSHVFHGLC 543

BLAST of MS009361 vs. ExPASy TrEMBL
Match: A0A6J1GQV5 (uncharacterized protein LOC111456279 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456279 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 3.6e-174
Identity = 355/565 (62.83%), Postives = 409/565 (72.39%), Query Frame = 0

Query: 1   MDAMGSDNLNLQNNLSDSIIPDDGNEELRCLGTMRTDSNFNGLHEEFSAAAGFHHSQFPD 60
           MD++  DNLNL+N LS   I DD NEE RCLGT+R+D+NFNGLHEE+SAA+ FHHSQFPD
Sbjct: 4   MDSIEKDNLNLRNKLS-GFISDDENEESRCLGTIRSDANFNGLHEEYSAASRFHHSQFPD 63

Query: 61  APVSFSHPNFAGSFSDSISYTDIDWSFDGSRLGLSSQNWSHSILTNAKSKTNAPFHVRTP 120
             VS SHPN AGSFSDSISYTDIDWSFDGSRLGLSSQ WSHSI  N K+KTNA F +R+P
Sbjct: 64  PSVS-SHPNLAGSFSDSISYTDIDWSFDGSRLGLSSQKWSHSIYANPKNKTNASFRIRSP 123

Query: 121 EVPSICRVPMRHQAAEGLHIVGETNNHNFQSSGCGEKIDLNITAIRSGFQPHSLEKLDSE 180
           E+ SICRVP R   AEG+H V  TNNHNF S+ C ++IDLNITAI S  QPH LE+LD E
Sbjct: 124 ELASICRVPARSHEAEGIHRV--TNNHNFHSAECEDQIDLNITAICSALQPHGLEELDDE 183

Query: 181 TL------KATTALNKADNISGFSD-------------NSVGLRCNNFINFTLHPSSVFQ 240
            L      KATTALNKAD ISGF D             NSVG+RC+NFINF+LH SSVFQ
Sbjct: 184 ALDVGCQTKATTALNKADKISGFKDNSGDIPNLTTFPQNSVGVRCSNFINFSLHQSSVFQ 243

Query: 241 PSIIVGEENPLPTPRNEDD--LNAAVGQSNGDIPNALKLAASSRTASSNSQIGLSSRSWS 300
           PSIIVG +NP PTP   DD  L  AV Q + DI N+LK  A   T S NS+I LSS+ W 
Sbjct: 244 PSIIVGGDNPFPTPSFTDDSGLTPAVVQGDEDILNSLK-NAPPPTESVNSKIELSSQCWF 303

Query: 301 TDLEPCPLRRDQSQQPSEHQSYIDTRNCSSHECSTFSPSLGIFATISVPDRSFELHQSAD 360
           TDLE C LR DQSQ+PSE +S+ D RN +S E  TF+PSLGI ATISVPD+S EL QSA 
Sbjct: 304 TDLESCLLRSDQSQRPSEQKSHPDVRNFAS-EFPTFAPSLGISATISVPDQSVELVQSAG 363

Query: 361 PSRPPQNVGNAATS----------GT------------------SFSSFTTLLSFHAQIS 420
           PS+PPQNVGNA  S          GT                  S  S  T +SF A ++
Sbjct: 364 PSQPPQNVGNALASVSGNQDAREFGTVEDSGIAKSNPNPPVFTSSPVSANTDISFQAHLA 423

Query: 421 QVLSVSASPDHMSSHVSIQPQTPKIDPDLQTQEQISRPIINDYLAPFPSKFEDLRVRSLR 480
           QVLSVSASPD ++SHV IQPQ  +IDP+     Q+ +PII++ +    SKFEDLR RS R
Sbjct: 424 QVLSVSASPD-ITSHVVIQPQKTEIDPN-----QVYQPIISESVPLVSSKFEDLRDRSFR 483

Query: 481 RSRSSHCYIKCQDLDQTLELSGERCFLCKRDLSFKPEGSLVLPKILPAVAVLPCRHVFHD 517
           RSR+S CYIKC+DLDQT +LSGERCF+CKRDLSFKPEGSL+LPKI P VAVLPC HVFH 
Sbjct: 484 RSRTSRCYIKCRDLDQTPKLSGERCFICKRDLSFKPEGSLILPKIPPTVAVLPCSHVFHG 543

BLAST of MS009361 vs. ExPASy TrEMBL
Match: A0A6J1JXH5 (uncharacterized protein LOC111488380 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111488380 PE=4 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 4.3e-167
Identity = 339/547 (61.97%), Postives = 390/547 (71.30%), Query Frame = 0

Query: 1   MDAMGSDNLNLQNNLSDSIIPDDGNEELRCLGTMRTDSNFNGLHEEFSAAAGFHHSQFPD 60
           MD+M  DNLNL+N LS   I DD NEE RCLGT+R+D+NFNGLHEE+SAA+G HHSQFPD
Sbjct: 4   MDSMEKDNLNLRNKLS-GFISDDENEESRCLGTIRSDANFNGLHEEYSAASGLHHSQFPD 63

Query: 61  APVSFSHPNFAGSFSDSISYTDIDWSFDGSRLGLSSQNWSHSILTNAKSKTNAPFHVRTP 120
             VS SHPN AGSFSDSISYTDIDWSFDGSRLGLSSQ WS+SI  N K+KTNA F +R+P
Sbjct: 64  PSVS-SHPNLAGSFSDSISYTDIDWSFDGSRLGLSSQKWSNSIYANPKNKTNASFRIRSP 123

Query: 121 EVPSICRVPMRHQAAEGLHIVGETNNHNFQSSGCGEKIDLNITAIRSGFQPHSLEKLDSE 180
           E+ SICRVP R   AEG+H V  TNNHNF S+ C ++IDLNITAI S  QPH LE+LD+E
Sbjct: 124 ELASICRVPARSHEAEGIHRV--TNNHNFHSAECEDQIDLNITAICSALQPHGLEELDAE 183

Query: 181 TL------KATTALNKADNISGFSD-------------NSVGLRCNNFINFTLHPSSVFQ 240
            L      KATTALNKAD ISGF D             NSVGLRC+NFINF+LH SSVFQ
Sbjct: 184 ALDVGCQTKATTALNKADKISGFKDNSGDIPNLTTFPQNSVGLRCSNFINFSLHQSSVFQ 243

Query: 241 PSIIVGEENPLPTPRNEDD--LNAAVGQSNGDIPNALKLAASSRTASSNSQIGLSSRSWS 300
           PSIIVG +NP PTP   DD  L  AV Q + DI N+LK A    T S NS+I LSS+ W 
Sbjct: 244 PSIIVGGDNPFPTPSFTDDSGLTPAVVQGDEDILNSLKNAPPLTTESVNSKIELSSQCWF 303

Query: 301 TDLEPCPLRRDQSQQPSEHQSYIDTRNCSSHECSTFSPSLGIFATISVPDRSFELHQSAD 360
           TDLE C LRRDQSQ+PSE +S+ D RN +S E  TF+PSLGI ATISVPD+S EL QSA 
Sbjct: 304 TDLESCLLRRDQSQRPSEQKSHPDVRNFTS-EFPTFAPSLGISATISVPDQSVELVQSAG 363

Query: 361 PSRPPQNVGNAATS----------GTSFSSFTTLLSFHAQISQVLSVSASPDHMSSHVSI 420
           PS+PPQNVGNA  S          GT   S     + H  +     VSA+ D     +S 
Sbjct: 364 PSQPPQNVGNALASVSGNQDAREFGTVEDSGIAKSNPHPPVFTSSPVSANTD-----ISF 423

Query: 421 QPQTPKIDPDLQTQEQISRPIINDYLAPFPSKFEDLRVRSLRRSRSSHCYIKCQDLDQTL 480
           Q              Q+ +PII++ +    SKFEDLR RS RRSR+S CYIKC+DLDQT 
Sbjct: 424 QAHL----------AQVYQPIISESVPLVSSKFEDLRDRSFRRSRTSRCYIKCRDLDQTP 483

Query: 481 ELSGERCFLCKRDLSFKPEGSLVLPKILPAVAVLPCRHVFHDLCLERITPQDQSEDPPCI 517
           +LSGERCF+CKRDLSFKPEGSLVLPKI P VA+LPC HVFH LCLER+TPQ+Q+EDPPC+
Sbjct: 484 KLSGERCFICKRDLSFKPEGSLVLPKIPPTVAILPCSHVFHGLCLERVTPQNQAEDPPCL 530

BLAST of MS009361 vs. TAIR 10
Match: AT5G18260.1 (RING/U-box superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 1.2e-07
Identity = 25/65 (38.46%), Postives = 39/65 (60.00%), Query Frame = 0

Query: 451 LSGERCFLCKRDLSFKPEGS---LVLPKILPAVAVLPCRHVFHDLCLERITPQDQSEDPP 510
           ++ +RC +CK+ LS K   S   ++  + +PA  V PC HV+H  CL++ TP  Q+ DP 
Sbjct: 187 MTHQRCGICKKLLSQKSPWSSYKILRSRDMPATGVFPCHHVYHVECLDKATPTAQTRDPS 246

Query: 511 CIPCA 513
           C  C+
Sbjct: 247 CPVCS 251

BLAST of MS009361 vs. TAIR 10
Match: AT2G21500.2 (RING/U-box superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 1.3e-06
Identity = 59/223 (26.46%), Postives = 87/223 (39.01%), Query Frame = 0

Query: 329 DRSFELHQSADPSRPPQNVGNAATSGTSFSSFTTLLSFHAQI-----SQVLSVSASPDHM 388
           D+SF    S D      +V    T  T+ S+  T     A++     SQ  S S SP   
Sbjct: 97  DQSFSRIASID------SVSEQITQKTNDSTDPTYYPSPAKLSLSLASQPSSFSVSPLSS 156

Query: 389 SSHVSIQPQTP---KIDPDLQTQEQISRPIINDYLAPFPSK------------------- 448
            SH+ + P +    K+ P  +  +Q+S   I  + +P  S                    
Sbjct: 157 QSHLPMPPASSSQLKLTPPPRLSKQVSYGQIFGFTSPSRSSATEERLGNESQSDGWSMQA 216

Query: 449 FEDLRVRSLRRSRS--SHCY----IKCQDLDQTLELSGERCFLCKRDLSFK---PEGSLV 508
           F ++   S R S S  + C+     K  +L        + C  C R LS K       + 
Sbjct: 217 FSEMMAYSRRESCSYDNECFGLRRDKIDNLGSRKSNDQQNCGACSRSLSEKSLLSSQKIF 276

Query: 509 LPKILPAVAVLPCRHVFHDLCLERITPQDQSEDPPCIPCAVGE 516
               L   A+L C HV+H  CLE++TP+    DP C  C +GE
Sbjct: 277 ATNELSVAAILACGHVYHSECLEQMTPEIDKFDPSCPICTLGE 313

BLAST of MS009361 vs. TAIR 10
Match: AT2G21500.1 (RING/U-box superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 1.3e-06
Identity = 59/223 (26.46%), Postives = 87/223 (39.01%), Query Frame = 0

Query: 329 DRSFELHQSADPSRPPQNVGNAATSGTSFSSFTTLLSFHAQI-----SQVLSVSASPDHM 388
           D+SF    S D      +V    T  T+ S+  T     A++     SQ  S S SP   
Sbjct: 97  DQSFSRIASID------SVSEQITQKTNDSTDPTYYPSPAKLSLSLASQPSSFSVSPLSS 156

Query: 389 SSHVSIQPQTP---KIDPDLQTQEQISRPIINDYLAPFPSK------------------- 448
            SH+ + P +    K+ P  +  +Q+S   I  + +P  S                    
Sbjct: 157 QSHLPMPPASSSQLKLTPPPRLSKQVSYGQIFGFTSPSRSSATEERLGNESQSDGWSMQA 216

Query: 449 FEDLRVRSLRRSRS--SHCY----IKCQDLDQTLELSGERCFLCKRDLSFK---PEGSLV 508
           F ++   S R S S  + C+     K  +L        + C  C R LS K       + 
Sbjct: 217 FSEMMAYSRRESCSYDNECFGLRRDKIDNLGSRKSNDQQNCGACSRSLSEKSLLSSQKIF 276

Query: 509 LPKILPAVAVLPCRHVFHDLCLERITPQDQSEDPPCIPCAVGE 516
               L   A+L C HV+H  CLE++TP+    DP C  C +GE
Sbjct: 277 ATNELSVAAILACGHVYHSECLEQMTPEIDKFDPSCPICTLGE 313

BLAST of MS009361 vs. TAIR 10
Match: AT4G39140.1 (RING/U-box superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.9e-05
Identity = 25/63 (39.68%), Postives = 33/63 (52.38%), Query Frame = 0

Query: 456 CFLCKRDLSFKPEGS---LVLPKILPAVAVLPCRHVFHDLCLERITPQDQSEDPPCIPCA 515
           C  C R LS K   S   + +   L   A+L C HV+H  CLE++TP+    DP C  C 
Sbjct: 260 CGACSRPLSEKSLWSSQKIFMTNELSVSAILACGHVYHGECLEQMTPEIDKFDPSCPICT 319

BLAST of MS009361 vs. TAIR 10
Match: AT4G39140.2 (RING/U-box superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.9e-05
Identity = 25/63 (39.68%), Postives = 33/63 (52.38%), Query Frame = 0

Query: 456 CFLCKRDLSFKPEGS---LVLPKILPAVAVLPCRHVFHDLCLERITPQDQSEDPPCIPCA 515
           C  C R LS K   S   + +   L   A+L C HV+H  CLE++TP+    DP C  C 
Sbjct: 260 CGACSRPLSEKSLWSSQKIFMTNELSVSAILACGHVYHGECLEQMTPEIDKFDPSCPICT 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023547724.19.4e-17763.06uncharacterized protein LOC111806582 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022991862.19.4e-17763.06uncharacterized protein LOC111488380 isoform X2 [Cucurbita maxima][more]
KAG7014196.12.1e-17662.70hypothetical protein SDJN02_24371, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022991860.11.0e-17563.01uncharacterized protein LOC111488380 isoform X1 [Cucurbita maxima][more]
XP_023547723.11.0e-17563.01uncharacterized protein LOC111806582 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1JN284.5e-17763.06uncharacterized protein LOC111488380 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JU435.0e-17663.01uncharacterized protein LOC111488380 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1GPA73.3e-17562.88uncharacterized protein LOC111456279 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1GQV53.6e-17462.83uncharacterized protein LOC111456279 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JXH54.3e-16761.97uncharacterized protein LOC111488380 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G18260.11.2e-0738.46RING/U-box superfamily protein [more]
AT2G21500.21.3e-0626.46RING/U-box superfamily protein [more]
AT2G21500.11.3e-0626.46RING/U-box superfamily protein [more]
AT4G39140.11.9e-0539.68RING/U-box superfamily protein [more]
AT4G39140.21.9e-0539.68RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 259..302
NoneNo IPR availablePANTHERPTHR31150:SF19ZINC FINGER, RING/FYVE/PHD-TYPE-RELATEDcoord: 182..516
NoneNo IPR availablePANTHERPTHR31150EXPRESSED PROTEINcoord: 182..516
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 456..511
score: 8.948558

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009361.1MS009361.1mRNA