MS009115 (gene) Bitter gourd (TR) v1

BLAST of MS009115 vs. NCBI nr
Match: XP_022133926.1 (dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 1015.4 bits (2624), Expect = 1.9e-292
Identity = 540/563 (95.91%), Postives = 544/563 (96.63%), Query Frame = 0

Query: 1   KLIMTSDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 60
           +   ++DSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE
Sbjct: 76  RFFSSTDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 135

Query: 61  EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED 120
           EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED
Sbjct: 136 EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED 195

Query: 121 GAQASSVEINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT 180
           GAQASSVEIN SKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT
Sbjct: 196 GAQASSVEINPSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT 255

Query: 181 LEFESLEEGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS 240
           LEFESLEEGYLAK LAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS
Sbjct: 256 LEFESLEEGYLAKKLAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS 315

Query: 241 TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS 300
           TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS
Sbjct: 316 TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS 375

Query: 301 SLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESKQNTPHLY 360
           SLSKEKSSPGV AQASSTASSESKSPIKQSD FEDLPNSQIRKVIAKRLLESKQNTPHLY
Sbjct: 376 SLSKEKSSPGVRAQASSTASSESKSPIKQSDPFEDLPNSQIRKVIAKRLLESKQNTPHLY 435

Query: 361 LSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCD 420
           LSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCD
Sbjct: 436 LSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCD 495

Query: 421 SIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTFSISN 480
           SIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGG+FSISN
Sbjct: 496 SIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGSFSISN 555

Query: 481 LGMFP------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFD 540
           LGMFP            AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFD
Sbjct: 556 LGMFPVDHFCAIINPPQAGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFD 615

Query: 541 GKVGGEFFSALQANFSNIQRLLL 552
           GKVGGEFFSALQANFSNIQRLLL
Sbjct: 616 GKVGGEFFSALQANFSNIQRLLL 638

BLAST of MS009115 vs. NCBI nr
Match: XP_022133927.1 (dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 1015.4 bits (2624), Expect = 1.9e-292
Identity = 540/563 (95.91%), Postives = 544/563 (96.63%), Query Frame = 0

Query: 1   KLIMTSDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 60
           +   ++DSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE
Sbjct: 75  RFFSSTDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 134

Query: 61  EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED 120
           EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED
Sbjct: 135 EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED 194

Query: 121 GAQASSVEINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT 180
           GAQASSVEIN SKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT
Sbjct: 195 GAQASSVEINPSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT 254

Query: 181 LEFESLEEGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS 240
           LEFESLEEGYLAK LAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS
Sbjct: 255 LEFESLEEGYLAKKLAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS 314

Query: 241 TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS 300
           TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS
Sbjct: 315 TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS 374

Query: 301 SLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESKQNTPHLY 360
           SLSKEKSSPGV AQASSTASSESKSPIKQSD FEDLPNSQIRKVIAKRLLESKQNTPHLY
Sbjct: 375 SLSKEKSSPGVRAQASSTASSESKSPIKQSDPFEDLPNSQIRKVIAKRLLESKQNTPHLY 434

Query: 361 LSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCD 420
           LSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCD
Sbjct: 435 LSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCD 494

Query: 421 SIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTFSISN 480
           SIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGG+FSISN
Sbjct: 495 SIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGSFSISN 554

Query: 481 LGMFP------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFD 540
           LGMFP            AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFD
Sbjct: 555 LGMFPVDHFCAIINPPQAGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFD 614

Query: 541 GKVGGEFFSALQANFSNIQRLLL 552
           GKVGGEFFSALQANFSNIQRLLL
Sbjct: 615 GKVGGEFFSALQANFSNIQRLLL 637

BLAST of MS009115 vs. NCBI nr
Match: XP_038884737.1 (dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 937.2 bits (2421), Expect = 6.5e-269
Identity = 502/565 (88.85%), Postives = 521/565 (92.21%), Query Frame = 0

Query: 1   KLIMTSDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 60
           +   T+DSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE
Sbjct: 80  RFFSTTDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 139

Query: 61  EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED 120
           EGFLAKILVPEGSKDVPVGQPIAITVEDP+DI+RV A+DVSGA+ IKDD  G Q+DKNED
Sbjct: 140 EGFLAKILVPEGSKDVPVGQPIAITVEDPEDINRVLANDVSGATDIKDDAEGEQQDKNED 199

Query: 121 GAQASSVEINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT 180
            AQASSVEIN+SKLPPHI+L MPALSPTMNQGNIA WRKKEGDKIEVGDVICEIETDKAT
Sbjct: 200 RAQASSVEINSSKLPPHIVLEMPALSPTMNQGNIATWRKKEGDKIEVGDVICEIETDKAT 259

Query: 181 LEFESLEEGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS 240
           LEFESLEEGYLAKILAPEGSKDVAVGKPIA+TVEDP DIESVK+AVSSS GIKEDK  H+
Sbjct: 260 LEFESLEEGYLAKILAPEGSKDVAVGKPIAITVEDPTDIESVKSAVSSSSGIKEDKPAHT 319

Query: 241 TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS 300
           T + DVGTSKGG+VARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS
Sbjct: 320 TVKNDVGTSKGGTVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS 379

Query: 301 --SLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESKQNTPH 360
             SLSK+K SP V AQASST SSESK  IKQSDSFEDLPNSQIRKVIAKRLLESKQNTPH
Sbjct: 380 KVSLSKDKRSPEVRAQASSTVSSESKPSIKQSDSFEDLPNSQIRKVIAKRLLESKQNTPH 439

Query: 361 LYLSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVF 420
           LYLSTDVILDPLLS RKDLKEKHDVKVSVNDIVIKAVAVALRNV GANAYWD+EKGEVVF
Sbjct: 440 LYLSTDVILDPLLSLRKDLKEKHDVKVSVNDIVIKAVAVALRNVRGANAYWDDEKGEVVF 499

Query: 421 CDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTFSI 480
           CDSIDISIAVATEKGLMTPIVRNAD KT+S ISSEVKELAEKARAGKLKPDEFQGGTFSI
Sbjct: 500 CDSIDISIAVATEKGLMTPIVRNADLKTISAISSEVKELAEKARAGKLKPDEFQGGTFSI 559

Query: 481 SNLGMFP------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRV 540
           SNLGMFP            AGILAVGRGNKVVEP+ GNDG ERPVVVNKMNLTLSADHRV
Sbjct: 560 SNLGMFPVDNFCAIINPPQAGILAVGRGNKVVEPIIGNDGVERPVVVNKMNLTLSADHRV 619

Query: 541 FDGKVGGEFFSALQANFSNIQRLLL 552
           FDGKVGGEF SALQANFS+IQRLLL
Sbjct: 620 FDGKVGGEFLSALQANFSSIQRLLL 644

BLAST of MS009115 vs. NCBI nr
Match: XP_038884738.1 (dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 937.2 bits (2421), Expect = 6.5e-269
Identity = 502/565 (88.85%), Postives = 521/565 (92.21%), Query Frame = 0

Query: 1   KLIMTSDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 60
           +   T+DSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE
Sbjct: 42  RFFSTTDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 101

Query: 61  EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED 120
           EGFLAKILVPEGSKDVPVGQPIAITVEDP+DI+RV A+DVSGA+ IKDD  G Q+DKNED
Sbjct: 102 EGFLAKILVPEGSKDVPVGQPIAITVEDPEDINRVLANDVSGATDIKDDAEGEQQDKNED 161

Query: 121 GAQASSVEINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT 180
            AQASSVEIN+SKLPPHI+L MPALSPTMNQGNIA WRKKEGDKIEVGDVICEIETDKAT
Sbjct: 162 RAQASSVEINSSKLPPHIVLEMPALSPTMNQGNIATWRKKEGDKIEVGDVICEIETDKAT 221

Query: 181 LEFESLEEGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS 240
           LEFESLEEGYLAKILAPEGSKDVAVGKPIA+TVEDP DIESVK+AVSSS GIKEDK  H+
Sbjct: 222 LEFESLEEGYLAKILAPEGSKDVAVGKPIAITVEDPTDIESVKSAVSSSSGIKEDKPAHT 281

Query: 241 TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS 300
           T + DVGTSKGG+VARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS
Sbjct: 282 TVKNDVGTSKGGTVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS 341

Query: 301 --SLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESKQNTPH 360
             SLSK+K SP V AQASST SSESK  IKQSDSFEDLPNSQIRKVIAKRLLESKQNTPH
Sbjct: 342 KVSLSKDKRSPEVRAQASSTVSSESKPSIKQSDSFEDLPNSQIRKVIAKRLLESKQNTPH 401

Query: 361 LYLSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVF 420
           LYLSTDVILDPLLS RKDLKEKHDVKVSVNDIVIKAVAVALRNV GANAYWD+EKGEVVF
Sbjct: 402 LYLSTDVILDPLLSLRKDLKEKHDVKVSVNDIVIKAVAVALRNVRGANAYWDDEKGEVVF 461

Query: 421 CDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTFSI 480
           CDSIDISIAVATEKGLMTPIVRNAD KT+S ISSEVKELAEKARAGKLKPDEFQGGTFSI
Sbjct: 462 CDSIDISIAVATEKGLMTPIVRNADLKTISAISSEVKELAEKARAGKLKPDEFQGGTFSI 521

Query: 481 SNLGMFP------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRV 540
           SNLGMFP            AGILAVGRGNKVVEP+ GNDG ERPVVVNKMNLTLSADHRV
Sbjct: 522 SNLGMFPVDNFCAIINPPQAGILAVGRGNKVVEPIIGNDGVERPVVVNKMNLTLSADHRV 581

Query: 541 FDGKVGGEFFSALQANFSNIQRLLL 552
           FDGKVGGEF SALQANFS+IQRLLL
Sbjct: 582 FDGKVGGEFLSALQANFSSIQRLLL 606

BLAST of MS009115 vs. NCBI nr
Match: XP_022990269.1 (dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 904.4 bits (2336), Expect = 4.7e-259
Identity = 489/567 (86.24%), Postives = 511/567 (90.12%), Query Frame = 0

Query: 1   KLIMTSDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 60
           +   ++DSSHAVL MPALSPTMNQGNIAKWRK EGDKIAVGDVLCEIETDKATLEFESLE
Sbjct: 77  RFFSSTDSSHAVLGMPALSPTMNQGNIAKWRKNEGDKIAVGDVLCEIETDKATLEFESLE 136

Query: 61  EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASV--IKDDTVGGQEDKN 120
           EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIH+V A+ VSGA    +KD+ +G Q++KN
Sbjct: 137 EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHKVLANGVSGAREPDVKDNAIGDQQEKN 196

Query: 121 EDGAQASSVEINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDK 180
           ED AQASS EIN SKLPPHI+L MPALSPTMNQGNIA WRKKEGDKIEVGDVICEIETDK
Sbjct: 197 EDRAQASSAEINPSKLPPHIVLEMPALSPTMNQGNIAIWRKKEGDKIEVGDVICEIETDK 256

Query: 181 ATLEFESLEEGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKAT 240
           ATLEFE LEEGYLAKIL PEGSKDV VGKPIA+TVEDPAD+ESVK+AVSSS GIKEDK  
Sbjct: 257 ATLEFECLEEGYLAKILEPEGSKDVPVGKPIAITVEDPADVESVKSAVSSSSGIKEDKPA 316

Query: 241 HSTTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKG 300
            ST + +VGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKG
Sbjct: 317 QSTVKNEVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKG 376

Query: 301 LS--SLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESKQNT 360
            S  SLS EK SP V +QASSTASSESKS +KQSD+FEDLPNSQIRKVIAKRLLESKQNT
Sbjct: 377 SSEVSLSIEKRSPEVRSQASSTASSESKSSVKQSDAFEDLPNSQIRKVIAKRLLESKQNT 436

Query: 361 PHLYLSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEV 420
           PHLYLSTDVILDPLLS RKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDN KGEV
Sbjct: 437 PHLYLSTDVILDPLLSLRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNTKGEV 496

Query: 421 VFCDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTF 480
           VFCDS+DISIAVATEKGLMTPIVRNADQKT+S ISSEVKELAEKAR GKLKPDEFQGGTF
Sbjct: 497 VFCDSVDISIAVATEKGLMTPIVRNADQKTLSAISSEVKELAEKARTGKLKPDEFQGGTF 556

Query: 481 SISNLGMFP------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADH 540
           SISNLGMFP            AGILAVGRGNKVVEPV   DG ERPVVVNKMNLTLSADH
Sbjct: 557 SISNLGMFPVDHFCAIINPPQAGILAVGRGNKVVEPVI-VDGIERPVVVNKMNLTLSADH 616

Query: 541 RVFDGKVGGEFFSALQANFSNIQRLLL 552
           RVFDGKVGGEF +ALQANFSNIQRLLL
Sbjct: 617 RVFDGKVGGEFLTALQANFSNIQRLLL 642

BLAST of MS009115 vs. ExPASy Swiss-Prot
Match: Q0WQF7 (Dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LTA3 PE=1 SV=2)

HSP 1 Score: 668.7 bits (1724), Expect = 5.7e-191
Identity = 371/562 (66.01%), Postives = 434/562 (77.22%), Query Frame = 0

Query: 5   TSDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLEEGFL 64
           T   S  VL MPALSPTM+ GN+ KW KKEGDK+ VGDVLCEIETDKAT+EFES EEGFL
Sbjct: 80  TGPISQTVLAMPALSPTMSHGNVVKWMKKEGDKVEVGDVLCEIETDKATVEFESQEEGFL 139

Query: 65  AKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDK-NEDGAQ 124
           AKILV EGSKD+PV +PIAI VE+ DDI  V A  + G    K++T   Q  K +E   Q
Sbjct: 140 AKILVTEGSKDIPVNEPIAIMVEEEDDIKNVPA-TIEGGRDGKEETSAHQVMKPDESTQQ 199

Query: 125 ASSVEINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKATLEF 184
            SS++ + S LPPH++L MPALSPTMNQGNIA W KKEGDKIEVGDVI EIETDKATLEF
Sbjct: 200 KSSIQPDASDLPPHVVLEMPALSPTMNQGNIAKWWKKEGDKIEVGDVIGEIETDKATLEF 259

Query: 185 ESLEEGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHSTTR 244
           ESLEEGYLAKIL PEGSKDVAVGKPIA+ VED   IE++K++ + S  +   K    +  
Sbjct: 260 ESLEEGYLAKILIPEGSKDVAVGKPIALIVEDAESIEAIKSSSAGSSEVDTVKEVPDSV- 319

Query: 245 KDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGK-GLSSL 304
            D  T +     +ISPAAKLLI EHGL+ASS++ASG +GTLLK DV+AAI SGK   SS 
Sbjct: 320 VDKPTERKAGFTKISPAAKLLILEHGLEASSIEASGPYGTLLKSDVVAAIASGKASKSSA 379

Query: 305 SKEKSSPGVHAQASSTASSESKSPIKQSD-SFEDLPNSQIRKVIAKRLLESKQNTPHLYL 364
           S +K  P    +  S +SS SK  + QSD ++ED PNSQIRK+IAKRLLESKQ  PHLYL
Sbjct: 380 STKKKQPS--KETPSKSSSTSKPSVTQSDNNYEDFPNSQIRKIIAKRLLESKQKIPHLYL 439

Query: 365 STDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCDS 424
            +DV+LDPLL+FRK+L+E H VKVSVNDIVIKAVAVALRNV  ANA+WD EKG++V CDS
Sbjct: 440 QSDVVLDPLLAFRKELQENHGVKVSVNDIVIKAVAVALRNVRQANAFWDAEKGDIVMCDS 499

Query: 425 IDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTFSISNL 484
           +DISIAVATEKGLMTPI++NADQK++S IS EVKELA+KAR+GKL P EFQGGTFSISNL
Sbjct: 500 VDISIAVATEKGLMTPIIKNADQKSISAISLEVKELAQKARSGKLAPHEFQGGTFSISNL 559

Query: 485 GMFP------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFDG 544
           GM+P            AGILAVGRGNKVVEPV G DG E+P VV KMN+TLSADHR+FDG
Sbjct: 560 GMYPVDNFCAIINPPQAGILAVGRGNKVVEPVIGLDGIEKPSVVTKMNVTLSADHRIFDG 619

Query: 545 KVGGEFFSALQANFSNIQRLLL 552
           +VG  F S L++NF +++RLLL
Sbjct: 620 QVGASFMSELRSNFEDVRRLLL 637

BLAST of MS009115 vs. ExPASy Swiss-Prot
Match: Q8BMF4 (Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial OS=Mus musculus OX=10090 GN=Dlat PE=1 SV=2)

HSP 1 Score: 358.2 bits (918), Expect = 1.6e-97
Identity = 230/560 (41.07%), Postives = 314/560 (56.07%), Query Frame = 0

Query: 10  HAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLEEGFLAKILV 69
           H  + +P+LSPTM  G IA+W KKEG+KI+ GD++ E+ETDKAT+ FESLEE ++AKILV
Sbjct: 90  HQKVPLPSLSPTMQAGTIARWEKKEGEKISEGDLIAEVETDKATVGFESLEECYMAKILV 149

Query: 70  PEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNEDGAQASSVEI 129
           PEG++DVPVG  I ITVE P DI       +  A+                   A S   
Sbjct: 150 PEGTRDVPVGSIICITVEKPQDIEAFKNYTLDLAAAAAPQAAPAAAPAPAAAPAAPSASA 209

Query: 130 NTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKATLEFESLEEG 189
             S  P H+ + +PALSPTM  G +  W KK G+K+  GD++ EIETDKAT+ FE  EEG
Sbjct: 210 PGSSYPTHMQIVLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEG 269

Query: 190 YLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKA------------ 249
           YLAKIL PEG++DV +G P+ + VE   DI +  +   + V   + +A            
Sbjct: 270 YLAKILVPEGTRDVPLGAPLCIIVEKQEDIAAFADYRPTEVTSLKPQAAPPAPPPVAAVP 329

Query: 250 -THSTTRKDVGTSKGGSVAR--ISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIK 309
            T          +  G   R  +SP AK L AE G+D + +K +G  G ++K D+     
Sbjct: 330 PTPQPVAPTPSAAPAGPKGRVFVSPLAKKLAAEKGIDLTQVKGTGPEGRIIKKDI----- 389

Query: 310 SGKGLSSLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESKQ 369
                 S    K++P   A  +      + +P   +  F D+P S IR+VIA+RL++SKQ
Sbjct: 390 -----DSFVPSKAAPAAAAAMAPPGPRVAPAP---AGVFTDIPISNIRRVIAQRLMQSKQ 449

Query: 370 NTPHLYLSTDVILDPLLSFRKDLKE--KHDVKVSVNDIVIKAVAVALRNVPGANAYWDN- 429
             PH YLS DV +  +L  RK+L +  +   K+SVND +IKA A+A   VP AN+ W + 
Sbjct: 450 TIPHYYLSVDVNMGEVLLVRKELNKMLEGKGKISVNDFIIKASALACLKVPEANSSWMDT 509

Query: 430 --EKGEVVFCDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPD 489
              +  VV     D+S+AV+T  GL+TPIV NA  K +  I+S+V  LA KAR GKL+P 
Sbjct: 510 VIRQNHVV-----DVSVAVSTPAGLITPIVFNAHIKGLETIASDVVSLASKAREGKLQPH 569

Query: 490 EFQGGTFSISNLGMF------------PAGILAVGRGNKVVEPVTGNDGTERPVVVNKMN 538
           EFQGGTF+ISNLGMF             A ILA+G     + P     G +   V + M+
Sbjct: 570 EFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLIPADNEKGFD---VASVMS 628

BLAST of MS009115 vs. ExPASy Swiss-Prot
Match: P36413 (Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=pdhC PE=1 SV=2)

HSP 1 Score: 350.9 bits (899), Expect = 2.6e-95
Identity = 220/569 (38.66%), Postives = 318/569 (55.89%), Query Frame = 0

Query: 13  LEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE-EGFLAKILVPE 72
           + MPALSP+M +GNI +W+KKEGD+I  GDV+ E+ETDKAT++F+  +  G+LAKIL+PE
Sbjct: 86  ITMPALSPSMTEGNIVQWKKKEGDQIKAGDVIAEVETDKATMDFQYEDGNGYLAKILIPE 145

Query: 73  GSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNEDGAQASSVEINT 132
           G+K + + +PIAI V   +DI   +  +   +S      V  +  K +  A   S    T
Sbjct: 146 GTKGIEINKPIAIIVSKKEDIES-AVKNYKPSSQASSTPVQEEAPKPKQEAPKKS----T 205

Query: 133 SKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKATLEFESLE-EGY 192
              P H ++GMPALSP+M  G IA+W KKEGD+I+ GD I E+ETDKAT++F+  +  GY
Sbjct: 206 KTYPAHKVVGMPALSPSMETGGIASWTKKEGDQIKAGDAIAEVETDKATMDFQYEDGNGY 265

Query: 193 LAKILAPEGSKDVAVGKPIAVTVEDPADIE----------------SVKNAVSSSVGIKE 252
           LAKIL P G+  + + +P+ + V++  D +                S + +  SS     
Sbjct: 266 LAKILVPGGTSGIQINQPVCIIVKNKEDCDKFADYSVEEQSSSSSSSSQESTPSSSSSSS 325

Query: 253 DKATHSTTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIK 312
            ++T S +     T K G     +PAA+   +  G D S++  +G +  +LK DVL  + 
Sbjct: 326 QESTPSQSSSQQTTRKSGERIFATPAARFEASSKGYDLSAINGTGPNNRILKADVLEFV- 385

Query: 313 SGKGLSSLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESKQ 372
                     +K       Q  +T +++  +    S  F D+P+S IRKV A RL ESKQ
Sbjct: 386 ---------PQKQEVAQQQQQQTTTTTKKPTTPTSSGEFTDIPHSNIRKVTAARLTESKQ 445

Query: 373 NTPHLYLSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKG 432
             PH YL+ +  +D LL  R +L   + VK+SVND ++KA A ALR+ P  N+ W ++  
Sbjct: 446 TIPHYYLTMECRVDKLLKLRSELNAMNTVKISVNDFIVKASAAALRDNPVVNSTWTDQ-- 505

Query: 433 EVVFCDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGG 492
            +    +IDI++AV T +GL TPIVR  D K ++ IS+ VK+LAEKA+ GKL P EF+ G
Sbjct: 506 FIRRYHNIDINVAVNTPQGLFTPIVRGVDMKGLNSISTSVKQLAEKAQNGKLHPSEFESG 565

Query: 493 TFSISNLGMF------------PAGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSA 552
           TF+ISNLGM              A ILAVG     V  V  N           +++TLS 
Sbjct: 566 TFTISNLGMLGIKQFAAVINPPQAAILAVGTTETRV--VLSNKPDSPYETATILSVTLSC 625

BLAST of MS009115 vs. ExPASy Swiss-Prot
Match: P08461 (Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial OS=Rattus norvegicus OX=10116 GN=Dlat PE=1 SV=3)

HSP 1 Score: 349.4 bits (895), Expect = 7.6e-95
Identity = 232/575 (40.35%), Postives = 318/575 (55.30%), Query Frame = 0

Query: 10  HAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLEEGFLAKILV 69
           H  + +P+LSPTM  G IA+W KKEG+KI+ GD++ E+ETDKAT+ FESLEE ++AKILV
Sbjct: 82  HQKVPLPSLSPTMQAGTIARWEKKEGEKISEGDLIAEVETDKATVGFESLEECYMAKILV 141

Query: 70  PEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNEDGAQASSVEI 129
           PEG++DVPVG  I ITVE P DI       +  A+                   A S   
Sbjct: 142 PEGTRDVPVGSIICITVEKPQDIEAFKNYTLDSATA-ATQAAPAPAAAPAAAPAAPSASA 201

Query: 130 NTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKATLEFESLEEG 189
             S  P H+ + +PALSPTM  G +  W KK G+K+  GD++ EIETDKAT+ FE  EEG
Sbjct: 202 PGSSYPVHMQIVLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEG 261

Query: 190 YLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATH---------- 249
           YLAKIL PEG++DV +G P+ + VE   DI +  +   + V   + +A            
Sbjct: 262 YLAKILVPEGTRDVPLGTPLCIIVEKQEDIAAFADYRPTEVTSLKPQAPPPVPPPVAAVP 321

Query: 250 ------STTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAI 309
                 + T         G V  +SP AK L AE G+D + +K +G  G ++K D+    
Sbjct: 322 PIPQPLAPTPSAAPAGPKGRVF-VSPLAKKLAAEKGIDLTQVKGTGPEGRIIKKDI---- 381

Query: 310 KSGKGLSSLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESK 369
                  S    K++P     A++ A    +     +  F D+P S IR+VIA+RL++SK
Sbjct: 382 ------DSFVPTKAAPA----AAAAAPPGPRVAPTPAGVFIDIPISNIRRVIAQRLMQSK 441

Query: 370 QNTPHLYLSTDVILDPLLSFRKDLKE--KHDVKVSVNDIVIKAVAVALRNVPGANAYWDN 429
           Q  PH YLS DV +  +L  RK+L +  +   K+SVND +IKA A+A   VP AN+ W +
Sbjct: 442 QTIPHYYLSVDVNMGEVLLVRKELNKMLEGKGKISVNDFIIKASALACLKVPEANSSWMD 501

Query: 430 ---EKGEVVFCDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKP 489
               +  VV     D+S+AV+T  GL+TPIV NA  K +  I+S+V  LA KAR GKL+P
Sbjct: 502 TVIRQNHVV-----DVSVAVSTPAGLITPIVFNAHIKGLETIASDVVSLASKAREGKLQP 561

Query: 490 DEFQGGTFSISNLGMF------------PAGILAVGRGNKVVEPVTGNDGTERPVVVNKM 549
            EFQGGTF+ISNLGMF             A ILA+G     + P     G +   V + M
Sbjct: 562 HEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLIPADNEKGFD---VASVM 621

Query: 550 NLTLSADHRVFDGKVGGEFFSALQANFSNIQRLLL 552
           ++TLS DHRV DG VG ++ +  +        +LL
Sbjct: 622 SVTLSCDHRVVDGAVGAQWLAEFKKYLEKPVTMLL 632

BLAST of MS009115 vs. ExPASy Swiss-Prot
Match: P10515 (Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial OS=Homo sapiens OX=9606 GN=DLAT PE=1 SV=3)

HSP 1 Score: 347.4 bits (890), Expect = 2.9e-94
Identity = 228/564 (40.43%), Postives = 310/564 (54.96%), Query Frame = 0

Query: 10  HAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLEEGFLAKILV 69
           H  + +P+LSPTM  G IA+W KKEGDKI  GD++ E+ETDKAT+ FESLEE ++AKILV
Sbjct: 91  HQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILV 150

Query: 70  PEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNEDGAQASSVEI 129
            EG++DVP+G  I ITV  P+DI       +  ++                     S + 
Sbjct: 151 AEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQA 210

Query: 130 NTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKATLEFESLEEG 189
             S  PPH+ + +PALSPTM  G +  W KK G+K+  GD++ EIETDKAT+ FE  EEG
Sbjct: 211 PGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEG 270

Query: 190 YLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSV-GIKED------------- 249
           YLAKIL PEG++DV +G P+ + VE  ADI +  +   + V  +K               
Sbjct: 271 YLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPTPPPVAAVP 330

Query: 250 ---KATHSTTRKDVGTSKGGSVAR--ISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVL 309
              +    T       +  G   R  +SP AK L  E G+D + +K +G  G + K D+ 
Sbjct: 331 PTPQPLAPTPSAPCPATPAGPKGRVFVSPLAKKLAVEKGIDLTQVKGTGPDGRITKKDI- 390

Query: 310 AAIKSGKGLSSLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLL 369
                     S    K +P   A    T    +  P   +  F D+P S IR+VIA+RL+
Sbjct: 391 ---------DSFVPSKVAPAPAAVVPPTGPGMAPVP---TGVFTDIPISNIRRVIAQRLM 450

Query: 370 ESKQNTPHLYLSTDVILDPLLSFRKDLKE--KHDVKVSVNDIVIKAVAVALRNVPGANAY 429
           +SKQ  PH YLS DV +  +L  RK+L +  +   K+SVND +IKA A+A   VP AN+ 
Sbjct: 451 QSKQTIPHYYLSIDVNMGEVLLVRKELNKILEGRSKISVNDFIIKASALACLKVPEANSS 510

Query: 430 WDN---EKGEVVFCDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGK 489
           W +    +  VV     D+S+AV+T  GL+TPIV NA  K V  I+++V  LA KAR GK
Sbjct: 511 WMDTVIRQNHVV-----DVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKAREGK 570

Query: 490 LKPDEFQGGTFSISNLGMF------------PAGILAVGRGNKVVEPVTGNDGTERPVVV 538
           L+P EFQGGTF+ISNLGMF             A ILA+G     + P     G +   V 
Sbjct: 571 LQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFD---VA 630

BLAST of MS009115 vs. ExPASy TrEMBL
Match: A0A6J1BY49 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Momordica charantia OX=3673 GN=LOC111006352 PE=3 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.1e-293
Identity = 540/563 (95.91%), Postives = 544/563 (96.63%), Query Frame = 0

Query: 1   KLIMTSDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 60
           +   ++DSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE
Sbjct: 75  RFFSSTDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 134

Query: 61  EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED 120
           EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED
Sbjct: 135 EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED 194

Query: 121 GAQASSVEINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT 180
           GAQASSVEIN SKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT
Sbjct: 195 GAQASSVEINPSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT 254

Query: 181 LEFESLEEGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS 240
           LEFESLEEGYLAK LAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS
Sbjct: 255 LEFESLEEGYLAKKLAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS 314

Query: 241 TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS 300
           TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS
Sbjct: 315 TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS 374

Query: 301 SLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESKQNTPHLY 360
           SLSKEKSSPGV AQASSTASSESKSPIKQSD FEDLPNSQIRKVIAKRLLESKQNTPHLY
Sbjct: 375 SLSKEKSSPGVRAQASSTASSESKSPIKQSDPFEDLPNSQIRKVIAKRLLESKQNTPHLY 434

Query: 361 LSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCD 420
           LSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCD
Sbjct: 435 LSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCD 494

Query: 421 SIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTFSISN 480
           SIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGG+FSISN
Sbjct: 495 SIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGSFSISN 554

Query: 481 LGMFP------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFD 540
           LGMFP            AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFD
Sbjct: 555 LGMFPVDHFCAIINPPQAGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFD 614

Query: 541 GKVGGEFFSALQANFSNIQRLLL 552
           GKVGGEFFSALQANFSNIQRLLL
Sbjct: 615 GKVGGEFFSALQANFSNIQRLLL 637

BLAST of MS009115 vs. ExPASy TrEMBL
Match: A0A6J1BWJ2 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Momordica charantia OX=3673 GN=LOC111006352 PE=3 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.1e-293
Identity = 540/563 (95.91%), Postives = 544/563 (96.63%), Query Frame = 0

Query: 1   KLIMTSDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 60
           +   ++DSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE
Sbjct: 76  RFFSSTDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 135

Query: 61  EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED 120
           EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED
Sbjct: 136 EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDKNED 195

Query: 121 GAQASSVEINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT 180
           GAQASSVEIN SKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT
Sbjct: 196 GAQASSVEINPSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKAT 255

Query: 181 LEFESLEEGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS 240
           LEFESLEEGYLAK LAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS
Sbjct: 256 LEFESLEEGYLAKKLAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHS 315

Query: 241 TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS 300
           TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS
Sbjct: 316 TTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKGLS 375

Query: 301 SLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESKQNTPHLY 360
           SLSKEKSSPGV AQASSTASSESKSPIKQSD FEDLPNSQIRKVIAKRLLESKQNTPHLY
Sbjct: 376 SLSKEKSSPGVRAQASSTASSESKSPIKQSDPFEDLPNSQIRKVIAKRLLESKQNTPHLY 435

Query: 361 LSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCD 420
           LSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCD
Sbjct: 436 LSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCD 495

Query: 421 SIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTFSISN 480
           SIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGG+FSISN
Sbjct: 496 SIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGSFSISN 555

Query: 481 LGMFP------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFD 540
           LGMFP            AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFD
Sbjct: 556 LGMFPVDHFCAIINPPQAGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFD 615

Query: 541 GKVGGEFFSALQANFSNIQRLLL 552
           GKVGGEFFSALQANFSNIQRLLL
Sbjct: 616 GKVGGEFFSALQANFSNIQRLLL 638

BLAST of MS009115 vs. ExPASy TrEMBL
Match: A0A6J1JRL5 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita maxima OX=3661 GN=LOC111487198 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.3e-259
Identity = 489/567 (86.24%), Postives = 511/567 (90.12%), Query Frame = 0

Query: 1   KLIMTSDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 60
           +   ++DSSHAVL MPALSPTMNQGNIAKWRK EGDKIAVGDVLCEIETDKATLEFESLE
Sbjct: 77  RFFSSTDSSHAVLGMPALSPTMNQGNIAKWRKNEGDKIAVGDVLCEIETDKATLEFESLE 136

Query: 61  EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASV--IKDDTVGGQEDKN 120
           EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIH+V A+ VSGA    +KD+ +G Q++KN
Sbjct: 137 EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHKVLANGVSGAREPDVKDNAIGDQQEKN 196

Query: 121 EDGAQASSVEINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDK 180
           ED AQASS EIN SKLPPHI+L MPALSPTMNQGNIA WRKKEGDKIEVGDVICEIETDK
Sbjct: 197 EDRAQASSAEINPSKLPPHIVLEMPALSPTMNQGNIAIWRKKEGDKIEVGDVICEIETDK 256

Query: 181 ATLEFESLEEGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKAT 240
           ATLEFE LEEGYLAKIL PEGSKDV VGKPIA+TVEDPAD+ESVK+AVSSS GIKEDK  
Sbjct: 257 ATLEFECLEEGYLAKILEPEGSKDVPVGKPIAITVEDPADVESVKSAVSSSSGIKEDKPA 316

Query: 241 HSTTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKG 300
            ST + +VGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKG
Sbjct: 317 QSTVKNEVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKG 376

Query: 301 LS--SLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESKQNT 360
            S  SLS EK SP V +QASSTASSESKS +KQSD+FEDLPNSQIRKVIAKRLLESKQNT
Sbjct: 377 SSEVSLSIEKRSPEVRSQASSTASSESKSSVKQSDAFEDLPNSQIRKVIAKRLLESKQNT 436

Query: 361 PHLYLSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEV 420
           PHLYLSTDVILDPLLS RKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDN KGEV
Sbjct: 437 PHLYLSTDVILDPLLSLRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNTKGEV 496

Query: 421 VFCDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTF 480
           VFCDS+DISIAVATEKGLMTPIVRNADQKT+S ISSEVKELAEKAR GKLKPDEFQGGTF
Sbjct: 497 VFCDSVDISIAVATEKGLMTPIVRNADQKTLSAISSEVKELAEKARTGKLKPDEFQGGTF 556

Query: 481 SISNLGMFP------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADH 540
           SISNLGMFP            AGILAVGRGNKVVEPV   DG ERPVVVNKMNLTLSADH
Sbjct: 557 SISNLGMFPVDHFCAIINPPQAGILAVGRGNKVVEPVI-VDGIERPVVVNKMNLTLSADH 616

Query: 541 RVFDGKVGGEFFSALQANFSNIQRLLL 552
           RVFDGKVGGEF +ALQANFSNIQRLLL
Sbjct: 617 RVFDGKVGGEFLTALQANFSNIQRLLL 642

BLAST of MS009115 vs. ExPASy TrEMBL
Match: A0A6J1JPN0 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita maxima OX=3661 GN=LOC111487198 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.3e-259
Identity = 489/567 (86.24%), Postives = 511/567 (90.12%), Query Frame = 0

Query: 1   KLIMTSDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 60
           +   ++DSSHAVL MPALSPTMNQGNIAKWRK EGDKIAVGDVLCEIETDKATLEFESLE
Sbjct: 76  RFFSSTDSSHAVLGMPALSPTMNQGNIAKWRKNEGDKIAVGDVLCEIETDKATLEFESLE 135

Query: 61  EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASV--IKDDTVGGQEDKN 120
           EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIH+V A+ VSGA    +KD+ +G Q++KN
Sbjct: 136 EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHKVLANGVSGAREPDVKDNAIGDQQEKN 195

Query: 121 EDGAQASSVEINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDK 180
           ED AQASS EIN SKLPPHI+L MPALSPTMNQGNIA WRKKEGDKIEVGDVICEIETDK
Sbjct: 196 EDRAQASSAEINPSKLPPHIVLEMPALSPTMNQGNIAIWRKKEGDKIEVGDVICEIETDK 255

Query: 181 ATLEFESLEEGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKAT 240
           ATLEFE LEEGYLAKIL PEGSKDV VGKPIA+TVEDPAD+ESVK+AVSSS GIKEDK  
Sbjct: 256 ATLEFECLEEGYLAKILEPEGSKDVPVGKPIAITVEDPADVESVKSAVSSSSGIKEDKPA 315

Query: 241 HSTTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKG 300
            ST + +VGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKG
Sbjct: 316 QSTVKNEVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKG 375

Query: 301 LS--SLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESKQNT 360
            S  SLS EK SP V +QASSTASSESKS +KQSD+FEDLPNSQIRKVIAKRLLESKQNT
Sbjct: 376 SSEVSLSIEKRSPEVRSQASSTASSESKSSVKQSDAFEDLPNSQIRKVIAKRLLESKQNT 435

Query: 361 PHLYLSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEV 420
           PHLYLSTDVILDPLLS RKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDN KGEV
Sbjct: 436 PHLYLSTDVILDPLLSLRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNTKGEV 495

Query: 421 VFCDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTF 480
           VFCDS+DISIAVATEKGLMTPIVRNADQKT+S ISSEVKELAEKAR GKLKPDEFQGGTF
Sbjct: 496 VFCDSVDISIAVATEKGLMTPIVRNADQKTLSAISSEVKELAEKARTGKLKPDEFQGGTF 555

Query: 481 SISNLGMFP------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADH 540
           SISNLGMFP            AGILAVGRGNKVVEPV   DG ERPVVVNKMNLTLSADH
Sbjct: 556 SISNLGMFPVDHFCAIINPPQAGILAVGRGNKVVEPVI-VDGIERPVVVNKMNLTLSADH 615

Query: 541 RVFDGKVGGEFFSALQANFSNIQRLLL 552
           RVFDGKVGGEF +ALQANFSNIQRLLL
Sbjct: 616 RVFDGKVGGEFLTALQANFSNIQRLLL 641

BLAST of MS009115 vs. ExPASy TrEMBL
Match: A0A6J1H962 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita moschata OX=3662 GN=LOC111461220 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.5e-258
Identity = 488/567 (86.07%), Postives = 510/567 (89.95%), Query Frame = 0

Query: 1   KLIMTSDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLE 60
           +   ++DSSHAVL MPALSPTMNQGNIAKWRK EGDKIAVGDVLCEIETDKATLEFESLE
Sbjct: 77  RFFSSTDSSHAVLGMPALSPTMNQGNIAKWRKNEGDKIAVGDVLCEIETDKATLEFESLE 136

Query: 61  EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASV--IKDDTVGGQEDKN 120
           EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIH+V A+ VSGA    +KD+ +G Q++KN
Sbjct: 137 EGFLAKILVPEGSKDVPVGQPIAITVEDPDDIHKVLANGVSGAREPDVKDNAIGDQQEKN 196

Query: 121 EDGAQASSVEINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDK 180
           ED AQASS EIN SKLPPHI+L MPALSPTMNQGNIA WRKKEGDKIEVGDVICEIETDK
Sbjct: 197 EDRAQASSAEINPSKLPPHIVLEMPALSPTMNQGNIAIWRKKEGDKIEVGDVICEIETDK 256

Query: 181 ATLEFESLEEGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKAT 240
           ATLEFE LEEGYLAKILAPEGSKDV VGKPIA+TVED AD+ESVK+AVSSS GIKEDK  
Sbjct: 257 ATLEFECLEEGYLAKILAPEGSKDVPVGKPIAITVEDLADVESVKSAVSSSSGIKEDKPA 316

Query: 241 HSTTRKDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKG 300
            ST + +VGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKG
Sbjct: 317 QSTVKNEVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGKG 376

Query: 301 LS--SLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESKQNT 360
            S  SLS EK SP V +QASSTASSESKS +KQSD+FED PNSQIRKVIAKRLLESKQNT
Sbjct: 377 SSEVSLSIEKRSPEVRSQASSTASSESKSSVKQSDAFEDFPNSQIRKVIAKRLLESKQNT 436

Query: 361 PHLYLSTDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEV 420
           PHLYLSTDVILDPLLS RKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDN KGEV
Sbjct: 437 PHLYLSTDVILDPLLSLRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNTKGEV 496

Query: 421 VFCDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTF 480
           VFCDS+DISIAVATEKGLMTPIVRNADQKT+S ISSEVKELAEKAR GKLKPDEFQGGTF
Sbjct: 497 VFCDSVDISIAVATEKGLMTPIVRNADQKTLSAISSEVKELAEKARTGKLKPDEFQGGTF 556

Query: 481 SISNLGMFP------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADH 540
           SISNLGMFP            AGILAVGRGNKVVEPV   DG ERPVVVNKMNLTLSADH
Sbjct: 557 SISNLGMFPVDQFCAIINPPQAGILAVGRGNKVVEPVI-VDGIERPVVVNKMNLTLSADH 616

Query: 541 RVFDGKVGGEFFSALQANFSNIQRLLL 552
           RVFDGKVGGEF +ALQANFSNIQRLLL
Sbjct: 617 RVFDGKVGGEFLTALQANFSNIQRLLL 642

BLAST of MS009115 vs. TAIR 10
Match: AT3G52200.1 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 668.7 bits (1724), Expect = 4.0e-192
Identity = 371/562 (66.01%), Postives = 434/562 (77.22%), Query Frame = 0

Query: 5   TSDSSHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLEEGFL 64
           T   S  VL MPALSPTM+ GN+ KW KKEGDK+ VGDVLCEIETDKAT+EFES EEGFL
Sbjct: 80  TGPISQTVLAMPALSPTMSHGNVVKWMKKEGDKVEVGDVLCEIETDKATVEFESQEEGFL 139

Query: 65  AKILVPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDK-NEDGAQ 124
           AKILV EGSKD+PV +PIAI VE+ DDI  V A  + G    K++T   Q  K +E   Q
Sbjct: 140 AKILVTEGSKDIPVNEPIAIMVEEEDDIKNVPA-TIEGGRDGKEETSAHQVMKPDESTQQ 199

Query: 125 ASSVEINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKATLEF 184
            SS++ + S LPPH++L MPALSPTMNQGNIA W KKEGDKIEVGDVI EIETDKATLEF
Sbjct: 200 KSSIQPDASDLPPHVVLEMPALSPTMNQGNIAKWWKKEGDKIEVGDVIGEIETDKATLEF 259

Query: 185 ESLEEGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHSTTR 244
           ESLEEGYLAKIL PEGSKDVAVGKPIA+ VED   IE++K++ + S  +   K    +  
Sbjct: 260 ESLEEGYLAKILIPEGSKDVAVGKPIALIVEDAESIEAIKSSSAGSSEVDTVKEVPDSV- 319

Query: 245 KDVGTSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGK-GLSSL 304
            D  T +     +ISPAAKLLI EHGL+ASS++ASG +GTLLK DV+AAI SGK   SS 
Sbjct: 320 VDKPTERKAGFTKISPAAKLLILEHGLEASSIEASGPYGTLLKSDVVAAIASGKASKSSA 379

Query: 305 SKEKSSPGVHAQASSTASSESKSPIKQSD-SFEDLPNSQIRKVIAKRLLESKQNTPHLYL 364
           S +K  P    +  S +SS SK  + QSD ++ED PNSQIRK+IAKRLLESKQ  PHLYL
Sbjct: 380 STKKKQPS--KETPSKSSSTSKPSVTQSDNNYEDFPNSQIRKIIAKRLLESKQKIPHLYL 439

Query: 365 STDVILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCDS 424
            +DV+LDPLL+FRK+L+E H VKVSVNDIVIKAVAVALRNV  ANA+WD EKG++V CDS
Sbjct: 440 QSDVVLDPLLAFRKELQENHGVKVSVNDIVIKAVAVALRNVRQANAFWDAEKGDIVMCDS 499

Query: 425 IDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTFSISNL 484
           +DISIAVATEKGLMTPI++NADQK++S IS EVKELA+KAR+GKL P EFQGGTFSISNL
Sbjct: 500 VDISIAVATEKGLMTPIIKNADQKSISAISLEVKELAQKARSGKLAPHEFQGGTFSISNL 559

Query: 485 GMFP------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFDG 544
           GM+P            AGILAVGRGNKVVEPV G DG E+P VV KMN+TLSADHR+FDG
Sbjct: 560 GMYPVDNFCAIINPPQAGILAVGRGNKVVEPVIGLDGIEKPSVVTKMNVTLSADHRIFDG 619

Query: 545 KVGGEFFSALQANFSNIQRLLL 552
           +VG  F S L++NF +++RLLL
Sbjct: 620 QVGASFMSELRSNFEDVRRLLL 637

BLAST of MS009115 vs. TAIR 10
Match: AT3G52200.2 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 667.9 bits (1722), Expect = 6.9e-192
Identity = 370/558 (66.31%), Postives = 433/558 (77.60%), Query Frame = 0

Query: 9   SHAVLEMPALSPTMNQGNIAKWRKKEGDKIAVGDVLCEIETDKATLEFESLEEGFLAKIL 68
           S  VL MPALSPTM+ GN+ KW KKEGDK+ VGDVLCEIETDKAT+EFES EEGFLAKIL
Sbjct: 160 SQTVLAMPALSPTMSHGNVVKWMKKEGDKVEVGDVLCEIETDKATVEFESQEEGFLAKIL 219

Query: 69  VPEGSKDVPVGQPIAITVEDPDDIHRVSADDVSGASVIKDDTVGGQEDK-NEDGAQASSV 128
           V EGSKD+PV +PIAI VE+ DDI  V A  + G    K++T   Q  K +E   Q SS+
Sbjct: 220 VTEGSKDIPVNEPIAIMVEEEDDIKNVPA-TIEGGRDGKEETSAHQVMKPDESTQQKSSI 279

Query: 129 EINTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKATLEFESLE 188
           + + S LPPH++L MPALSPTMNQGNIA W KKEGDKIEVGDVI EIETDKATLEFESLE
Sbjct: 280 QPDASDLPPHVVLEMPALSPTMNQGNIAKWWKKEGDKIEVGDVIGEIETDKATLEFESLE 339

Query: 189 EGYLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVGIKEDKATHSTTRKDVG 248
           EGYLAKIL PEGSKDVAVGKPIA+ VED   IE++K++ + S  +   K    +   D  
Sbjct: 340 EGYLAKILIPEGSKDVAVGKPIALIVEDAESIEAIKSSSAGSSEVDTVKEVPDSV-VDKP 399

Query: 249 TSKGGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAIKSGK-GLSSLSKEK 308
           T +     +ISPAAKLLI EHGL+ASS++ASG +GTLLK DV+AAI SGK   SS S +K
Sbjct: 400 TERKAGFTKISPAAKLLILEHGLEASSIEASGPYGTLLKSDVVAAIASGKASKSSASTKK 459

Query: 309 SSPGVHAQASSTASSESKSPIKQSD-SFEDLPNSQIRKVIAKRLLESKQNTPHLYLSTDV 368
             P    +  S +SS SK  + QSD ++ED PNSQIRK+IAKRLLESKQ  PHLYL +DV
Sbjct: 460 KQPS--KETPSKSSSTSKPSVTQSDNNYEDFPNSQIRKIIAKRLLESKQKIPHLYLQSDV 519

Query: 369 ILDPLLSFRKDLKEKHDVKVSVNDIVIKAVAVALRNVPGANAYWDNEKGEVVFCDSIDIS 428
           +LDPLL+FRK+L+E H VKVSVNDIVIKAVAVALRNV  ANA+WD EKG++V CDS+DIS
Sbjct: 520 VLDPLLAFRKELQENHGVKVSVNDIVIKAVAVALRNVRQANAFWDAEKGDIVMCDSVDIS 579

Query: 429 IAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPDEFQGGTFSISNLGMFP 488
           IAVATEKGLMTPI++NADQK++S IS EVKELA+KAR+GKL P EFQGGTFSISNLGM+P
Sbjct: 580 IAVATEKGLMTPIIKNADQKSISAISLEVKELAQKARSGKLAPHEFQGGTFSISNLGMYP 639

Query: 489 ------------AGILAVGRGNKVVEPVTGNDGTERPVVVNKMNLTLSADHRVFDGKVGG 548
                       AGILAVGRGNKVVEPV G DG E+P VV KMN+TLSADHR+FDG+VG 
Sbjct: 640 VDNFCAIINPPQAGILAVGRGNKVVEPVIGLDGIEKPSVVTKMNVTLSADHRIFDGQVGA 699

Query: 549 EFFSALQANFSNIQRLLL 552
            F S L++NF +++RLLL
Sbjct: 700 SFMSELRSNFEDVRRLLL 713

BLAST of MS009115 vs. TAIR 10
Match: AT3G13930.1 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 287.0 bits (733), Expect = 3.3e-77
Identity = 184/457 (40.26%), Postives = 264/457 (57.77%), Query Frame = 0

Query: 130 NTSKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKATLEFESLEEG 189
           ++S LPPH  +GMP+LSPTM +GNIA W KKEGDK+  G+V+CE+ETDKAT+E E +EEG
Sbjct: 104 SSSDLPPHQEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEG 163

Query: 190 YLAKILAPEGSKDVAVGKPIAVTVEDPADIESVKN-AVSSSVG------------IKEDK 249
           +LAKI+  EG+K++ VG+ IA+TVED  DI+  K+   SS  G             KE+K
Sbjct: 164 FLAKIVKEEGAKEIQVGEVIAITVEDEDDIQKFKDYTPSSDTGPAAPEAKPAPSLPKEEK 223

Query: 250 ATHSTTRKDVGTSKGGSVAR-----ISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLA 309
                +  +   SK  S         SP A+ L  ++ +  SS+K +G  G ++K DV  
Sbjct: 224 VEKPASAPEAKISKPSSAPSEDRIFASPLARKLAEDNNVPLSSIKGTGPEGRIVKADVED 283

Query: 310 AIKSGKGLSSLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLE 369
            + SG      SKE +       A  +   +SK P   +  + D+P++QIRKV A RL  
Sbjct: 284 FLASG------SKETT-------AKPSKQVDSKVP---ALDYVDIPHTQIRKVTASRLAF 343

Query: 370 SKQNTPHLYLSTDVILDPLLSFRKDLKEKHDV----KVSVNDIVIKAVAVALRNVPGANA 429
           SKQ  PH YL+ D  +D ++  R  L    +     ++SVND+VIKA A+ALR VP  N+
Sbjct: 344 SKQTIPHYYLTVDTCVDKMMGLRSQLNSFQEASGGKRISVNDLVIKAAALALRKVPQCNS 403

Query: 430 YWDNEKGEVVFCDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLK 489
            W +E   +    +++I++AV TE GL  P+V++AD+K +S I  EV+ LA+KA+   LK
Sbjct: 404 SWTDE--YIRQFKNVNINVAVQTENGLYVPVVKDADKKGLSTIGEEVRFLAQKAKENSLK 463

Query: 490 PDEFQGGTFSISNLG------MF-------PAGILAVGRGNKVVEPVTGNDGTERPVVVN 549
           P++++GGTF++SNLG       F        A ILA+G   K V P TG D      V +
Sbjct: 464 PEDYEGGTFTVSNLGGPFGIKQFCAVINPPQAAILAIGSAEKRVVPGTGPDQYN---VAS 523

Query: 550 KMNLTLSADHRVFDGKVGGEFFSALQANFSNIQRLLL 552
            M++TLS DHRV DG +G E+  A +      + +LL
Sbjct: 524 YMSVTLSCDHRVIDGAIGAEWLKAFKGYIETPESMLL 539

BLAST of MS009115 vs. TAIR 10
Match: AT1G54220.1 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 285.8 bits (730), Expect = 7.4e-77
Identity = 181/455 (39.78%), Postives = 260/455 (57.14%), Query Frame = 0

Query: 132 SKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKATLEFESLEEGYL 191
           S LPPH  +GMP+LSPTM +GNIA W KKEGDK+  G+V+CE+ETDKAT+E E +EEGYL
Sbjct: 106 SDLPPHQEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYL 165

Query: 192 AKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVG-------------IKEDKAT 251
           AKI+  EGSK++ VG+ IA+TVED  DI   K+   SS                KE+K  
Sbjct: 166 AKIVKAEGSKEIQVGEVIAITVEDEEDIGKFKDYTPSSTADAAPTKAEPTPAPPKEEKVK 225

Query: 252 HSTTRKDVGTSK-----GGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAI 311
             ++  +   SK      G     SP A+ L  ++ +  S ++ +G  G ++K D+    
Sbjct: 226 QPSSPPEPKASKPSTPPTGDRVFASPLARKLAEDNNVPLSDIEGTGPEGRIVKADI---- 285

Query: 312 KSGKGLSSLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESK 371
                     +  +S G  A A  + S++SK+P   +  + D+P+SQIRKV A RL  SK
Sbjct: 286 ---------DEYLASSGKGATAKPSKSTDSKAP---ALDYVDIPHSQIRKVTASRLAFSK 345

Query: 372 QNTPHLYLSTDVILDPLLSFRKDLKEKHDV----KVSVNDIVIKAVAVALRNVPGANAYW 431
           Q  PH YL+ D  +D L++ R  L    +     ++SVND+V+KA A+ALR VP  N+ W
Sbjct: 346 QTIPHYYLTVDTCVDKLMALRSQLNSFKEASGGKRISVNDLVVKAAALALRKVPQCNSSW 405

Query: 432 DNEKGEVVFCDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPD 491
            ++   +    +++I++AV TE GL  P+V++AD+K +S I  EV+ LA+KA+   LKP+
Sbjct: 406 TDD--YIRQFKNVNINVAVQTENGLYVPVVKDADRKGLSTIGEEVRLLAQKAKENSLKPE 465

Query: 492 EFQGGTFSISNLG------MF-------PAGILAVGRGNKVVEPVTGNDGTERPVVVNKM 551
           +++GGTF++SNLG       F        A ILAVG   K V P  G D        + M
Sbjct: 466 DYEGGTFTVSNLGGPFGIKQFCAVVNPPQAAILAVGSAEKRVVPGNGPDQFN---FASYM 525

BLAST of MS009115 vs. TAIR 10
Match: AT1G54220.2 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 285.8 bits (730), Expect = 7.4e-77
Identity = 181/455 (39.78%), Postives = 260/455 (57.14%), Query Frame = 0

Query: 132 SKLPPHIILGMPALSPTMNQGNIANWRKKEGDKIEVGDVICEIETDKATLEFESLEEGYL 191
           S LPPH  +GMP+LSPTM +GNIA W KKEGDK+  G+V+CE+ETDKAT+E E +EEGYL
Sbjct: 106 SDLPPHQEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYL 165

Query: 192 AKILAPEGSKDVAVGKPIAVTVEDPADIESVKNAVSSSVG-------------IKEDKAT 251
           AKI+  EGSK++ VG+ IA+TVED  DI   K+   SS                KE+K  
Sbjct: 166 AKIVKAEGSKEIQVGEVIAITVEDEEDIGKFKDYTPSSTADAAPTKAEPTPAPPKEEKVK 225

Query: 252 HSTTRKDVGTSK-----GGSVARISPAAKLLIAEHGLDASSLKASGSHGTLLKGDVLAAI 311
             ++  +   SK      G     SP A+ L  ++ +  S ++ +G  G ++K D+    
Sbjct: 226 QPSSPPEPKASKPSTPPTGDRVFASPLARKLAEDNNVPLSDIEGTGPEGRIVKADI---- 285

Query: 312 KSGKGLSSLSKEKSSPGVHAQASSTASSESKSPIKQSDSFEDLPNSQIRKVIAKRLLESK 371
                     +  +S G  A A  + S++SK+P   +  + D+P+SQIRKV A RL  SK
Sbjct: 286 ---------DEYLASSGKGATAKPSKSTDSKAP---ALDYVDIPHSQIRKVTASRLAFSK 345

Query: 372 QNTPHLYLSTDVILDPLLSFRKDLKEKHDV----KVSVNDIVIKAVAVALRNVPGANAYW 431
           Q  PH YL+ D  +D L++ R  L    +     ++SVND+V+KA A+ALR VP  N+ W
Sbjct: 346 QTIPHYYLTVDTCVDKLMALRSQLNSFKEASGGKRISVNDLVVKAAALALRKVPQCNSSW 405

Query: 432 DNEKGEVVFCDSIDISIAVATEKGLMTPIVRNADQKTVSMISSEVKELAEKARAGKLKPD 491
            ++   +    +++I++AV TE GL  P+V++AD+K +S I  EV+ LA+KA+   LKP+
Sbjct: 406 TDD--YIRQFKNVNINVAVQTENGLYVPVVKDADRKGLSTIGEEVRLLAQKAKENSLKPE 465

Query: 492 EFQGGTFSISNLG------MF-------PAGILAVGRGNKVVEPVTGNDGTERPVVVNKM 551
           +++GGTF++SNLG       F        A ILAVG   K V P  G D        + M
Sbjct: 466 DYEGGTFTVSNLGGPFGIKQFCAVVNPPQAAILAVGSAEKRVVPGNGPDQFN---FASYM 525