MS008782 (gene) Bitter gourd (TR) v1

Overview
NameMS008782
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncalmodulin-like
Locationscaffold4: 3683877 .. 3684140 (+)
RNA-Seq ExpressionMS008782
SyntenyMS008782
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCGCAAAGGAAAGCAGTACCGGCCGAAGCTGTCGCGAGAGCAGCTCAGCCAAATCTTCAGAAACCACGACGACGATGGCGACGGGCGCCTCAACGCCGACGAGCTCACCGAAGCCTTCCGCTACATCGGTGCATTTCTCCCCTCCTACAGAGCTCGCCACGCCCTTTTCCACACTGATTCCGATCGAGATGGATTCATCGGCGAGAAAGATTTCGAATCTCTCCTTAATTATGCCGAGAAACGTAAATACACCATCATT

mRNA sequence

ATGGCTCGCAAAGGAAAGCAGTACCGGCCGAAGCTGTCGCGAGAGCAGCTCAGCCAAATCTTCAGAAACCACGACGACGATGGCGACGGGCGCCTCAACGCCGACGAGCTCACCGAAGCCTTCCGCTACATCGGTGCATTTCTCCCCTCCTACAGAGCTCGCCACGCCCTTTTCCACACTGATTCCGATCGAGATGGATTCATCGGCGAGAAAGATTTCGAATCTCTCCTTAATTATGCCGAGAAACGTAAATACACCATCATT

Coding sequence (CDS)

ATGGCTCGCAAAGGAAAGCAGTACCGGCCGAAGCTGTCGCGAGAGCAGCTCAGCCAAATCTTCAGAAACCACGACGACGATGGCGACGGGCGCCTCAACGCCGACGAGCTCACCGAAGCCTTCCGCTACATCGGTGCATTTCTCCCCTCCTACAGAGCTCGCCACGCCCTTTTCCACACTGATTCCGATCGAGATGGATTCATCGGCGAGAAAGATTTCGAATCTCTCCTTAATTATGCCGAGAAACGTAAATACACCATCATT

Protein sequence

MARKGKQYRPKLSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFIGEKDFESLLNYAEKRKYTII
Homology
BLAST of MS008782 vs. NCBI nr
Match: KAG6608481.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037808.1 putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 147.5 bits (371), Expect = 5.4e-32
Identity = 75/90 (83.33%), Postives = 79/90 (87.78%), Query Frame = 0

Query: 1  MARKGK--QYRPKLSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALF 60
          MARK K  Q RPKLSREQLS IFRNHD DGDGRL+A ELTEAFRYIGAFLPSYRA HAL 
Sbjct: 1  MARKNKMSQPRPKLSREQLSVIFRNHDGDGDGRLSATELTEAFRYIGAFLPSYRAHHALR 60

Query: 61 HTDSDRDGFIGEKDFESLLNYAEKRKYTII 89
          +TDSDRDGFIGEKDFESL+NY EKR YTI+
Sbjct: 61 YTDSDRDGFIGEKDFESLVNYTEKRHYTIV 90

BLAST of MS008782 vs. NCBI nr
Match: KAG7024248.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 146.7 bits (369), Expect = 9.1e-32
Identity = 74/90 (82.22%), Postives = 77/90 (85.56%), Query Frame = 0

Query: 1  MARKGKQY--RPKLSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALF 60
          MARK K Y  RPKLSREQLS IFRNHD DGDGRL+  ELT AFRYIGAFLPSYRA HAL 
Sbjct: 1  MARKSKTYQPRPKLSREQLSVIFRNHDGDGDGRLSTTELTAAFRYIGAFLPSYRALHALL 60

Query: 61 HTDSDRDGFIGEKDFESLLNYAEKRKYTII 89
          HTDSD DGFIGEKDFE L+NYAEKRKYT+I
Sbjct: 61 HTDSDGDGFIGEKDFELLVNYAEKRKYTVI 90

BLAST of MS008782 vs. NCBI nr
Match: KAG6591370.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 144.1 bits (362), Expect = 5.9e-31
Identity = 73/88 (82.95%), Postives = 75/88 (85.23%), Query Frame = 0

Query: 1  MARKGKQY--RPKLSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALF 60
          MARK K Y  RPKLSREQLS IFRNHD DGDGRL+  ELT AFRYIGAFLPSYRA HAL 
Sbjct: 1  MARKSKTYQPRPKLSREQLSVIFRNHDGDGDGRLSTTELTAAFRYIGAFLPSYRALHALL 60

Query: 61 HTDSDRDGFIGEKDFESLLNYAEKRKYT 87
          HTDSD DGFIGEKDFE L+NYAEKRKYT
Sbjct: 61 HTDSDGDGFIGEKDFELLVNYAEKRKYT 88

BLAST of MS008782 vs. NCBI nr
Match: KGN55185.1 (hypothetical protein Csa_012915 [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 1.7e-30
Identity = 71/90 (78.89%), Postives = 77/90 (85.56%), Query Frame = 0

Query: 1  MARKGKQYRP--KLSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALF 60
          M+RK K Y P  KLSREQL  IFRNHD D DGRL+  ELTEAFRYIGAFLP+YRARHAL 
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HTDSDRDGFIGEKDFESLLNYAEKRKYTII 89
          H DSDRDGFIGEKDFESL+NYAE+R+YTII
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

BLAST of MS008782 vs. NCBI nr
Match: KAA0064376.1 (putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK20211.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa])

HSP 1 Score: 137.1 bits (344), Expect = 7.2e-29
Identity = 67/90 (74.44%), Postives = 75/90 (83.33%), Query Frame = 0

Query: 1  MARKGKQYRP--KLSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALF 60
          M+R+ K Y P  +LSREQL  IFRNHD D DGRL+  ELTEAFRYIGAFLP YRARHALF
Sbjct: 1  MSRRSKTYHPRARLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPPYRARHALF 60

Query: 61 HTDSDRDGFIGEKDFESLLNYAEKRKYTII 89
          H DSDRDGFI EKDFE L+NYAE+R+YT+I
Sbjct: 61 HADSDRDGFISEKDFELLVNYAEQRQYTVI 90

BLAST of MS008782 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 2.8e-07
Identity = 25/65 (38.46%), Postives = 41/65 (63.08%), Query Frame = 0

Query: 13  SREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFIGEKD 72
           S+E+L Q+F + D DGDG+++A EL   F  +G ++    A+ A+   D+D DG +G +D
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFED 120

Query: 73  FESLL 78
           F  L+
Sbjct: 121 FVGLM 125

BLAST of MS008782 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.4e-06
Identity = 24/58 (41.38%), Postives = 36/58 (62.07%), Query Frame = 0

Query: 16 QLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFIGEKDF 74
          +L Q+FR +D +GDG+++A+EL    R +GA L     R  +   DSDRDGF+   +F
Sbjct: 5  ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 62

BLAST of MS008782 vs. ExPASy Swiss-Prot
Match: Q6L4D4 (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 GN=CML15 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.7e-04
Identity = 21/68 (30.88%), Postives = 36/68 (52.94%), Query Frame = 0

Query: 9   RPKLSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFI 68
           RP  + ++  ++FR  D +GDGR++  EL   FR +G  +        +   DSD DG+I
Sbjct: 48  RPPAAEDETERVFRKFDANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYI 107

Query: 69  GEKDFESL 77
              +F ++
Sbjct: 108 SLGEFAAI 115

BLAST of MS008782 vs. ExPASy Swiss-Prot
Match: Q39419 (Polcalcin Bet v 4 OS=Betula pendula OX=3505 GN=BETV4 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.7e-04
Identity = 21/60 (35.00%), Postives = 37/60 (61.67%), Query Frame = 0

Query: 14 REQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFIGEKDF 73
          + +  +IF+  D +GDG+++A EL EA + +G+  P    +H +   D+D DGFI  ++F
Sbjct: 9  KAERERIFKRFDANGDGKISAAELGEALKTLGSITPD-EVKHMMAEIDTDGDGFISFQEF 67

BLAST of MS008782 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 44.7 bits (104), Expect = 6.4e-04
Identity = 18/66 (27.27%), Postives = 37/66 (56.06%), Query Frame = 0

Query: 11  KLSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFIGE 70
           ++   +L ++F+  D +GDGR+  +EL ++   +G ++P       +   D++ DG +  
Sbjct: 60  RIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDI 119

Query: 71  KDFESL 77
            +FESL
Sbjct: 120 DEFESL 125

BLAST of MS008782 vs. ExPASy TrEMBL
Match: A0A0A0KZR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639730 PE=4 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.3e-31
Identity = 71/90 (78.89%), Postives = 77/90 (85.56%), Query Frame = 0

Query: 1  MARKGKQYRP--KLSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALF 60
          M+RK K Y P  KLSREQL  IFRNHD D DGRL+  ELTEAFRYIGAFLP+YRARHAL 
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HTDSDRDGFIGEKDFESLLNYAEKRKYTII 89
          H DSDRDGFIGEKDFESL+NYAE+R+YTII
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

BLAST of MS008782 vs. ExPASy TrEMBL
Match: A0A5A7VDB9 (Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003480 PE=4 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.5e-29
Identity = 67/90 (74.44%), Postives = 75/90 (83.33%), Query Frame = 0

Query: 1  MARKGKQYRP--KLSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALF 60
          M+R+ K Y P  +LSREQL  IFRNHD D DGRL+  ELTEAFRYIGAFLP YRARHALF
Sbjct: 1  MSRRSKTYHPRARLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPPYRARHALF 60

Query: 61 HTDSDRDGFIGEKDFESLLNYAEKRKYTII 89
          H DSDRDGFI EKDFE L+NYAE+R+YT+I
Sbjct: 61 HADSDRDGFISEKDFELLVNYAEQRQYTVI 90

BLAST of MS008782 vs. ExPASy TrEMBL
Match: A0A5A7UY46 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00260 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.2e-13
Identity = 40/78 (51.28%), Postives = 53/78 (67.95%), Query Frame = 0

Query: 11 KLSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFIGE 70
          KL +E++ QIFR HD +GDGRL+  EL+ AF   GAF P YRA   LF  D D DGFI E
Sbjct: 14 KLQKEEMKQIFREHDFNGDGRLSIKELSRAFGSFGAFFPLYRAAFGLFVADDDGDGFISE 73

Query: 71 KDFESLLNYAEKRKYTII 89
          ++ E +++YA K KY ++
Sbjct: 74 QELEKVVDYAIKCKYNLL 91

BLAST of MS008782 vs. ExPASy TrEMBL
Match: A5AWP8 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_023174 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.7e-13
Identity = 38/76 (50.00%), Postives = 53/76 (69.74%), Query Frame = 0

Query: 12 LSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFIGEK 71
          L  +QL +IF+ HD +GDG+LN  EL +AF+++GA +P +RA  AL H D+D DG I EK
Sbjct: 15 LPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTDGDGCISEK 74

Query: 72 DFESLLNYAEKRKYTI 88
          + + L+ YA K  YTI
Sbjct: 75 EMKDLVEYAAKFGYTI 90

BLAST of MS008782 vs. ExPASy TrEMBL
Match: A0A5N6RQV2 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018544 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.0e-12
Identity = 39/75 (52.00%), Postives = 54/75 (72.00%), Query Frame = 0

Query: 13 SREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFIGEKD 72
          +R+QL  IFR+HD DGDG+L+  E+  AF Y+G+   SYRA  AL H DSD DG++GE++
Sbjct: 16 TRDQLMNIFRSHDLDGDGKLSRKEVKAAFGYLGSRWDSYRAVLALLHADSDHDGYVGEEE 75

Query: 73 FESLLNYAEKRKYTI 88
           + L++YA K  YTI
Sbjct: 76 LKKLVDYALKCGYTI 90

BLAST of MS008782 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 55.8 bits (133), Expect = 2.0e-08
Identity = 25/65 (38.46%), Postives = 41/65 (63.08%), Query Frame = 0

Query: 13  SREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFIGEKD 72
           S+E+L Q+F + D DGDG+++A EL   F  +G ++    A+ A+   D+D DG +G +D
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFED 120

Query: 73  FESLL 78
           F  L+
Sbjct: 121 FVGLM 125

BLAST of MS008782 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 44.7 bits (104), Expect = 4.6e-05
Identity = 18/66 (27.27%), Postives = 37/66 (56.06%), Query Frame = 0

Query: 11  KLSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFIGE 70
           ++   +L ++F+  D +GDGR+  +EL ++   +G ++P       +   D++ DG +  
Sbjct: 60  RIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDI 119

Query: 71  KDFESL 77
            +FESL
Sbjct: 120 DEFESL 125

BLAST of MS008782 vs. TAIR 10
Match: AT3G59440.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 43.5 bits (101), Expect = 1.0e-04
Identity = 19/60 (31.67%), Postives = 34/60 (56.67%), Query Frame = 0

Query: 17  LSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFIGEKDFESL 76
           L ++F+  D +GDGR+  +EL ++   +G F+P       +   D++ DG +   +FESL
Sbjct: 52  LKRVFQMFDKNGDGRITKEELNDSLENLGIFMPDKDLIQMIQKMDANGDGCVDINEFESL 111

BLAST of MS008782 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 41.6 bits (96), Expect = 3.9e-04
Identity = 19/65 (29.23%), Postives = 36/65 (55.38%), Query Frame = 0

Query: 12 LSREQLSQIFRNHDDDGDGRLNADELTEAFRYIGAFLPSYRARHALFHTDSDRDGFIGEK 71
          + R +LS++F+  D +GDG++  +EL + F+ +G  +P       +   D + DG +   
Sbjct: 1  MDRGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDID 60

Query: 72 DFESL 77
          +F SL
Sbjct: 61 EFGSL 65

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6608481.15.4e-3283.33Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037808.1... [more]
KAG7024248.19.1e-3282.22Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6591370.15.9e-3182.95Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia][more]
KGN55185.11.7e-3078.89hypothetical protein Csa_012915 [Cucumis sativus][more]
KAA0064376.17.2e-2974.44putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK20211.1 pu... [more]
Match NameE-valueIdentityDescription
Q8L3R22.8e-0738.46Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ... [more]
Q0DJV61.4e-0641.38Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q6L4D41.7e-0430.88Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q394191.7e-0435.00Polcalcin Bet v 4 OS=Betula pendula OX=3505 GN=BETV4 PE=1 SV=1[more]
O228456.4e-0427.27Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KZR68.3e-3178.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639730 PE=4 SV=1[more]
A0A5A7VDB93.5e-2974.44Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A5A7UY461.2e-1351.28Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A5AWP82.7e-1350.00Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_023174 PE=4 SV=1[more]
A0A5N6RQV21.0e-1252.00Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018544 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G50770.12.0e-0838.46calmodulin-like 41 [more]
AT2G43290.14.6e-0527.27Calcium-binding EF-hand family protein [more]
AT3G59440.11.0e-0431.67Calcium-binding EF-hand family protein [more]
AT4G12860.13.9e-0429.23EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.238.10coord: 1..84
e-value: 7.3E-12
score: 47.5
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 11..83
NoneNo IPR availablePANTHERPTHR23050:SF451EF-HAND PAIR PROTEINcoord: 11..83
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 18..42
e-value: 1.6E-5
score: 24.3
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 12..47
score: 13.397589
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 25..37
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 61..73
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 8..79

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS008782.1MS008782.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding