MS008718 (gene) Bitter gourd (TR) v1

BLAST of MS008718 vs. NCBI nr
Match: XP_022146589.1 (cytochrome P450 81D1-like [Momordica charantia])

HSP 1 Score: 1047.0 bits (2706), Expect = 5.6e-302
Identity = 516/525 (98.29%), Postives = 522/525 (99.43%), Query Frame = 0

Query: 1   VHSIILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRT 60
           +HS ILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRT
Sbjct: 1   MHSKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRT 60

Query: 61  LADISNRHGPVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNL 120
           LADISNRHGPVVFLRFGSRPVLVVSSPS AEECLTKNDVVFANRPRLLISKCFGYNNTNL
Sbjct: 61  LADISNRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNL 120

Query: 121 VWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSF 180
           VWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSF
Sbjct: 121 VWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSF 180

Query: 181 FDLTFNVMLRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGF 240
           FDLTFNVMLRMIAGKRFYGDDVDDADE+AKIFRELQAESTRLTGISNIGDFVPMMTWLGF
Sbjct: 181 FDLTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGF 240

Query: 241 GWSVEKKMMDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQES 300
           GWSVEKKMMDCHYKRDAFMQ+LIEEQRKRSTAETESEIDDSFRDGR+KTMIEVLLELQES
Sbjct: 241 GWSVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQES 300

Query: 301 EPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLI 360
           EPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEID+QVGQDRLI
Sbjct: 301 EPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDBQVGQDRLI 360

Query: 361 DESDMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHND 420
           DESDMAHLPYLRGII ETFRMYPPAPLMVPHQSSEDC+VGGHHIPRGTMLYVNLWAIHND
Sbjct: 361 DESDMAHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHND 420

Query: 421 PKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCF 480
           PKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCF
Sbjct: 421 PKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCF 480

Query: 481 EWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKT 526
           EWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKT
Sbjct: 481 EWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKT 525

BLAST of MS008718 vs. NCBI nr
Match: XP_038896683.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 823.9 bits (2127), Expect = 7.7e-235
Identity = 403/523 (77.06%), Postives = 462/523 (88.34%), Query Frame = 0

Query: 3   SIILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLA 62
           +++  A+GAIFLY PLFLVLYILTEHFLHKIRNLPPTPFPS+P++GHL+LL KPIYR+L 
Sbjct: 30  NLMAMAIGAIFLYIPLFLVLYILTEHFLHKIRNLPPTPFPSIPILGHLHLLKKPIYRSLY 89

Query: 63  DISNRHGPVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVW 122
           +IS+R+GPVVFLR G R +L+VSSPS AEECLTKND+VFANRPRLLISKCFGYNNTNLVW
Sbjct: 90  NISHRYGPVVFLRLGYRSILIVSSPSIAEECLTKNDIVFANRPRLLISKCFGYNNTNLVW 149

Query: 123 SSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFD 182
           SSYGDHWR+LRRI TVE+LSTHRL +LS VRLEEVRSLI++L K ENQ  ++ M+H FFD
Sbjct: 150 SSYGDHWRNLRRICTVEILSTHRLHMLSIVRLEEVRSLIQRLLKCENQ--LVNMKHEFFD 209

Query: 183 LTFNVMLRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGW 242
           LTFN+MLRMI GKRFYGDDVDD D EAK+FRELQAESTRL+G SNIGDF+P++ WLGFG 
Sbjct: 210 LTFNLMLRMIVGKRFYGDDVDDVD-EAKLFRELQAESTRLSGKSNIGDFIPLIKWLGFGS 269

Query: 243 SVEKKMMDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEP 302
           ++EK MM+C  +RD  MQSLIE+ R+R      +EIDDSFRDGRKKTMIEVLLELQESEP
Sbjct: 270 TLEKDMMNCQNRRDTLMQSLIEQHRRRRM----TEIDDSFRDGRKKTMIEVLLELQESEP 329

Query: 303 DHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDE 362
           + Y DE+I+ALML++L AGTETS + MEWALSLLLNHP +LKKAQ EID QVG +R IDE
Sbjct: 330 EQYNDETIRALMLLMLVAGTETSGSIMEWALSLLLNHPTILKKAQTEIDHQVGHERSIDE 389

Query: 363 SDMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPK 422
           SDMAHLPYLRGIINET RMYPPAPL+VPH+SSEDC+VGG+HIPRGT+LY+N+WAI NDPK
Sbjct: 390 SDMAHLPYLRGIINETLRMYPPAPLLVPHESSEDCSVGGYHIPRGTVLYINIWAIQNDPK 449

Query: 423 IWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEW 482
           IWA+P KFDPDRF+  ES+   Y FNLMPFG GRRGCPGEGLGLR+IGLVLGSLIQCFEW
Sbjct: 450 IWAHPRKFDPDRFNGHESE--NYRFNLMPFGLGRRGCPGEGLGLRMIGLVLGSLIQCFEW 509

Query: 483 ERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKT 526
           ERP E+LVDMTEGIA++MPKAQ L AKCRPRPII NL SQTK+
Sbjct: 510 ERPSEQLVDMTEGIAITMPKAQHLHAKCRPRPIIVNLLSQTKS 543

BLAST of MS008718 vs. NCBI nr
Match: XP_022940352.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 811.2 bits (2094), Expect = 5.1e-231
Identity = 397/518 (76.64%), Postives = 467/518 (90.15%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRH 68
           MGAI +Y PLF VLY+LTE FLH+IRNLPPTPF SLP+IGHL+LL KP+YRTLA+ISNR+
Sbjct: 1   MGAIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 69  GPVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDH 128
           GPVVFLRFGSR VLVVSSPSAAEECL+KNDVVFA+RPRLLISK FGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 129 WRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVM 188
           WR+LRRISTVE+LSTHRLQ+L+ VRL+EV+SLI +LAK ENQ  +  M ++FFDLTFNVM
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKGENQ--VQDMNNAFFDLTFNVM 180

Query: 189 LRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 248
           LRMI GKRFYG+DV+DAD EA  FRELQ++ST+L G +N+GDF+P+M+WLG GW+ EK++
Sbjct: 181 LRMIVGKRFYGEDVEDAD-EAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEI 240

Query: 249 MDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEPDHYTDE 308
           M+CH KRDAFMQ+LI + + R+    E+E D+SFRDGRKKT+IEVLLELQES+PD Y DE
Sbjct: 241 MECHQKRDAFMQNLINQHKNRA---IETEPDNSFRDGRKKTLIEVLLELQESDPDQYNDE 300

Query: 309 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 368
           +I+ALMLVLL AGTETS+NTMEWALSL+LN+PEVL+KAQ+EID +VG DRL+DESDMA++
Sbjct: 301 TIRALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANV 360

Query: 369 PYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 428
           P+LRGIINET RMYPPAPLMVPH+SS+DC+VGG+HIPRGTML+VNLWAIHNDPKIWA+P 
Sbjct: 361 PFLRGIINETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPR 420

Query: 429 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 488
           KFDPDRF ++ES+K   GF LMPFGSGRRGCPGEGLGLR+IGLVLGSLIQCFEWERPGE+
Sbjct: 421 KFDPDRFVKVESEK--LGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEE 480

Query: 489 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKT 526
           LVDMTEG+AVSMPKA+ LQAKC+PRPI+  L  SQTK+
Sbjct: 481 LVDMTEGVAVSMPKARHLQAKCKPRPIVDRLHLSQTKS 510

BLAST of MS008718 vs. NCBI nr
Match: XP_023523281.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 803.5 bits (2074), Expect = 1.1e-228
Identity = 393/518 (75.87%), Postives = 466/518 (89.96%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRH 68
           MGAI +Y PLFLVLY+LTE FLH+IRNLPPTPF SLP+IGHL+LL KP+YRTLA+ISNR+
Sbjct: 1   MGAIIVYIPLFLVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 69  GPVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDH 128
           GPVVFLRFGSR VLVVSSPSAAEECL+KNDVVFA+RPRLLISK FGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 129 WRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVM 188
           WR+LRRISTVE+LSTHRLQ+L+ VR +EV+SLI +LA+ ENQ  +  M ++FFDLTFNVM
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRSDEVKSLIGRLARGENQ--VQDMNNAFFDLTFNVM 180

Query: 189 LRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 248
           LRMI GKRFYG+DV+DAD EA  FRELQ++ST+L G +N+ DF+P+M+WLG GW+ EK++
Sbjct: 181 LRMIVGKRFYGEDVEDAD-EAHRFRELQSKSTQLGGKTNMEDFIPLMSWLGLGWAAEKEI 240

Query: 249 MDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEPDHYTDE 308
           M+CH KRDAFMQ+LI + + R+    E+E D+SFRDGRKKT+IEVL+ELQES+PD Y DE
Sbjct: 241 MECHQKRDAFMQNLINQHKNRA---IETEPDNSFRDGRKKTLIEVLVELQESDPDQYNDE 300

Query: 309 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 368
           +I+ALMLVLL AGTETS+NTMEWALSL+LN+PEVL+KAQ+EID +VG DRL+DESDMA++
Sbjct: 301 TIRALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANV 360

Query: 369 PYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 428
           P+LRGIINET RMYPPAPLMVPH+SS+DC+VGG+ IPRGTML+VNLWAIHNDPKIWA+P 
Sbjct: 361 PFLRGIINETLRMYPPAPLMVPHESSKDCSVGGYQIPRGTMLFVNLWAIHNDPKIWADPR 420

Query: 429 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 488
           KFDPDRF +++S+K  +GF LMPFGSGRRGCPGEGLGLR+IGLVLGSLIQCFEWERPGE 
Sbjct: 421 KFDPDRFVKVDSEK--HGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGED 480

Query: 489 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKT 526
           LVDMTEG+AVSMPKA+ LQAKC+PRPI+A L  SQTK+
Sbjct: 481 LVDMTEGVAVSMPKARHLQAKCKPRPIVARLHLSQTKS 510

BLAST of MS008718 vs. NCBI nr
Match: XP_008452620.2 (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 802.4 bits (2071), Expect = 2.4e-228
Identity = 388/518 (74.90%), Postives = 455/518 (87.84%), Query Frame = 0

Query: 8   AMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNR 67
           AMGA+FLY PLFLVLY+LT+HFLH IRNLPP PFPSLP++GHL+LLNKPIYR L +ISNR
Sbjct: 4   AMGALFLYIPLFLVLYLLTKHFLHNIRNLPPXPFPSLPILGHLHLLNKPIYRALYNISNR 63

Query: 68  HGPVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGD 127
           +GPVVFLR GSR VL+VSS SAAEECLTKND++FANRPRLL+SKCFGYNNTNLVWSSYGD
Sbjct: 64  YGPVVFLRLGSRSVLIVSSASAAEECLTKNDIIFANRPRLLLSKCFGYNNTNLVWSSYGD 123

Query: 128 HWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNV 187
            WR+LRRI TVE+LSTHRL +LS VR EEVRSLI++L K ENQ  ++ M+H FFDL+FN+
Sbjct: 124 IWRNLRRICTVEILSTHRLHMLSVVRFEEVRSLIQRLLKRENQ--VVNMKHVFFDLSFNL 183

Query: 188 MLRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKK 247
           MLRMI GKRFYGDDVDD  EEAK+FRELQA+S+ L+G SN+GDF+P+M+WLGF  ++EK+
Sbjct: 184 MLRMIVGKRFYGDDVDDV-EEAKLFRELQAKSSHLSGKSNMGDFIPLMSWLGFDSTLEKE 243

Query: 248 MMDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEPDHYTD 307
           MMDC   RDA MQSLIE+ R+R       EIDDSFRDGRKKT+IEVLLELQESEP+ Y D
Sbjct: 244 MMDCQNGRDALMQSLIEQHRRRRIV----EIDDSFRDGRKKTLIEVLLELQESEPEQYND 303

Query: 308 ESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAH 367
           E+I+ LML++L AGTETS + MEWALSLLLNHPE+LKKAQ+EID+QVG  R I+ESDMA 
Sbjct: 304 ETIRGLMLLMLVAGTETSGSIMEWALSLLLNHPEILKKAQIEIDNQVGNQRPIEESDMAR 363

Query: 368 LPYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKIWANP 427
           LPYLRGIINET RMYPPAPL+VPH+SS+DC+VGG+H+PRGTMLY+N+WAI NDPKIWA+P
Sbjct: 364 LPYLRGIINETLRMYPPAPLLVPHESSQDCSVGGYHVPRGTMLYINIWAIQNDPKIWAHP 423

Query: 428 TKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGE 487
            +FDPDRF+ +ES+K  Y FNLMPFG GRRGCPGEGLGLR+IGLVLGSLIQCFEWERP E
Sbjct: 424 KEFDPDRFNDVESEK--YKFNLMPFGLGRRGCPGEGLGLRMIGLVLGSLIQCFEWERPTE 483

Query: 488 KLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKT 526
           +LVD+TEGIA++MPKAQ L AKCRPRP+I N+  Q K+
Sbjct: 484 ELVDLTEGIAITMPKAQNLHAKCRPRPMIVNILPQAKS 512

BLAST of MS008718 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 4.8e-139
Identity = 249/491 (50.71%), Postives = 337/491 (68.64%), Query Frame = 0

Query: 34  RNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRHGPVVFLRFGSRPVLVVSSPSAAEEC 93
           RNLPP+P  +LP+IGHL+L+ K ++R+L D+S ++G V  L+ G+R VLVVSSP+AAEEC
Sbjct: 30  RNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEEC 89

Query: 94  LTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHWRSLRRISTVEVLSTHRLQILSTVR 153
            TKND+VFANRP  ++ K  GYN T +V S YG+HWR+LRR++ VE+ S   L    ++R
Sbjct: 90  FTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIR 149

Query: 154 LEEVRSLIRKLAKSENQD-KILTMRHSFFDLTFNVMLRMIAGKRFYGDDVDDADEEAKIF 213
            +EV+ L+  L +S  QD   + M+    +L+FNV +RM+AGKR++G DVD   +EAK+F
Sbjct: 150 EDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTMRMVAGKRYFGQDVD--SDEAKLF 209

Query: 214 RELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKMMDCHYKRDAFMQSLIEEQRKRSTA 273
           R L  E     G SN GDFVP + W+ F  + EKK+     + DAF+Q LI E R     
Sbjct: 210 RALIGEVFEHAGASNPGDFVPFLRWIDFK-NYEKKVSKISQEMDAFLQRLIHESRINK-- 269

Query: 274 ETESEIDDSFRDGRKKTMIEVLLELQESEPDHYTDESIKALMLVLLTAGTETSVNTMEWA 333
                           TMI+ LL LQES+P++YTD+ IK +++VLL AGT+TS  T+EWA
Sbjct: 270 -------------NNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDTSAVTVEWA 329

Query: 334 LSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHLPYLRGIINETFRMYPPAPLMVPHQ 393
           +SLLLNHPE L+KA+ EI+ QVG +RLI+E D+  L YL  II+ETFR+ P AP++VPH+
Sbjct: 330 MSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPAAPMLVPHE 389

Query: 394 SSEDCTVGGHHIPRGTMLYVNLWAIHNDPKIWANPTKFDPDRFDRLESDKYGYGFNLMPF 453
           SS+DC V G+ +P+GT+L VN WAIH DP+ W  PT F P+R   +E +       LMPF
Sbjct: 390 SSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGVELEP----SKLMPF 449

Query: 454 GSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEKLVDMTEGIAVSMPKAQPLQAKCRP 513
           G GRR CPG GL  R++GL LG+LIQCFEW+R GE  +DM EG  ++MPKAQPL+A C+P
Sbjct: 450 GMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLEALCKP 498

Query: 514 RPIIANLPSQT 524
           R I+  + S+T
Sbjct: 510 RNILHKVVSET 498

BLAST of MS008718 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 9.4e-127
Identity = 246/518 (47.49%), Postives = 337/518 (65.06%), Query Frame = 0

Query: 12  IFLYFPLFLVLYILTEHFLH-KIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADIS----- 71
           + LY    L+  I++  FL  K +NLPP+P   LP+IGHL LL  PI+RTL   S     
Sbjct: 8   VVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 67

Query: 72  NRHGPVVFLRFGSRPVLVVSSPS-AAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSS 131
           N  G V+ LR GSR V VVSS   AAEEC  KNDVV ANRP+++I K  GYNNTN++ + 
Sbjct: 68  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 127

Query: 132 YGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDK-ILTMRHSFFDL 191
           YGDHWR+LRR+ T+E+ STHRL     VR +EVR LI +L++     K ++ ++    DL
Sbjct: 128 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDL 187

Query: 192 TFNVMLRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWS 251
           TFN ++RM+ GKR+YG++  D +EEAK  R+L A+    T   N  D+VP++       S
Sbjct: 188 TFNNIMRMMTGKRYYGEETTD-EEEAKRVRKLVADVGANTSSGNAVDYVPILRLFS---S 247

Query: 252 VEKKMMDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEPD 311
            E ++     + D F+Q LI+++R +    T              TMI+ LL LQ+S+ +
Sbjct: 248 YENRVKKLGEETDKFLQGLIDDKRGQQETGT--------------TMIDHLLVLQKSDIE 307

Query: 312 HYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDES 371
           +YTD+ IK ++L+++ AGT TS  T+EWALS LLNHP+V+ KA+ EID++VG DRLI+E+
Sbjct: 308 YYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEA 367

Query: 372 DMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKI 431
           D++ LPYL+ I+ ET R++P  PL+VPH +SEDC +G + +PRGT L VN WAIH DP  
Sbjct: 368 DLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNT 427

Query: 432 WANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWE 491
           W +P  F P+RF++ E  +      L+ FG GRR CPG GL  RI+GL LGSLIQCFEWE
Sbjct: 428 WDDPDSFKPERFEKEEEAQ-----KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWE 487

Query: 492 RPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPS 522
           R G   VDM EG+  ++PKA PL+A C+ RP +  + S
Sbjct: 488 RVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKIIS 502

BLAST of MS008718 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 2.1e-126
Identity = 242/516 (46.90%), Postives = 328/516 (63.57%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIR----NLPPTPFPSLPLIGHLYLLNKPIYRTLADI 68
           M  I+L   LF     L+   L   R    NLPP+P    P+IGHL+LL  P++R    +
Sbjct: 8   METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67

Query: 69  SN--RHGPVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVW 128
           S    +  +  L  GSR V VVSS + AEEC TKNDVV ANRP  L+ K  GYN+T +V 
Sbjct: 68  SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127

Query: 129 SSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKI-LTMRHSFF 188
           ++YGD WR+LRRI T+E+ S+ RL    ++R +E+R LI  LAK+     + + M+  F 
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187

Query: 189 DLTFNVMLRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFG 248
            LT N ++RM+AGKRFYGD  ++ D EAK  R+L AE     G  N  D+ P++ ++   
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTEN-DNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--- 247

Query: 249 WSVEKKMMDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESE 308
            + EK +     + D F+QSL+ E+R                  +  TMI+ LL LQE++
Sbjct: 248 TNYEKHVKKLAGRVDEFLQSLVNEKRVEKV--------------KGNTMIDHLLSLQETQ 307

Query: 309 PDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLID 368
           PD+YTD  IK ++LV++ AGT+TS  T+EWA+S LLNHPEVL+KA+ EIDDQ+G DRL++
Sbjct: 308 PDYYTDVIIKGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVE 367

Query: 369 ESDMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDP 428
           E D+  LPYL+ I++ET R+YP AP+++PH +SEDC V G+ +PRGT++ VN WAIH DP
Sbjct: 368 EQDIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDP 427

Query: 429 KIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFE 488
           K+W  P KF P+RF++   DK      LMPFG GRR CPG GL  R++ L LGSL+QCFE
Sbjct: 428 KLWEEPEKFKPERFEKKGEDK-----KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFE 487

Query: 489 WERPGEKLVDMTEG-IAVSMPKAQPLQAKCRPRPII 517
           WER  EK +DM E     +M KA  LQA C+ RPI+
Sbjct: 488 WERVEEKYLDMRESEKGTTMRKATSLQAMCKARPIV 500

BLAST of MS008718 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 451.1 bits (1159), Expect = 1.8e-125
Identity = 235/514 (45.72%), Postives = 338/514 (65.76%), Query Frame = 0

Query: 5   ILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADI 64
           IL+ +     Y  LF +  I+      K +NLPP P PSLP+IG+L+ L +P++RT   +
Sbjct: 3   ILSLLSYSVFYLALFFIFNIVIR--ARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGL 62

Query: 65  SNRHGPVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSS 124
           S ++G V  L FGSR V+VVSS S  ++C TKNDVV ANRPR L  K   YN T L  +S
Sbjct: 63  SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 122

Query: 125 YGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLA-KSENQDKILTMRHSFFDL 184
           YG+HWR+LRRI+ ++VLS HR+   S +R +E + LI +LA  S      + +    +D+
Sbjct: 123 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDM 182

Query: 185 TFNVMLRMIAGKRFYGDDVDDAD-EEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGW 244
           TFN ++RMI+GKR+YG+D D +D +EA  FR++ +E  +L+G +N  DF+P++ +L F  
Sbjct: 183 TFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFE- 242

Query: 245 SVEKKMMDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEP 304
           ++EK++ D   K DAF++ LIEE R      T+ E        R  TMI+ LL LQ+S+P
Sbjct: 243 NLEKRLKDISGKTDAFLRGLIEEHR------TKKE--------RANTMIDHLLNLQDSQP 302

Query: 305 DHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDE 364
           ++YTD+ IK L L +L AGT++S  T+EW++S LLNHPEVLKK + E+D  VGQDRL+DE
Sbjct: 303 EYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDE 362

Query: 365 SDMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPK 424
           SD+  L YL+ +INET R+Y PAPL++PH +S++C +GG+ +P+ T++ +N WAIH DP+
Sbjct: 363 SDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPE 422

Query: 425 IWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEW 484
           +W   T F P+RF     +K G    L+ FG GRR CPGEGL +R I + L  LIQCF+W
Sbjct: 423 LWTEATTFKPERF-----EKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDW 482

Query: 485 ERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPII 517
           +      +D+ E    ++ K  PL+A C+ RP+I
Sbjct: 483 KLINGDKIDLAERDGFTLTKLVPLKAMCKSRPVI 493

BLAST of MS008718 vs. ExPASy Swiss-Prot
Match: Q0WTF4 (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.8e-125
Identity = 235/515 (45.63%), Postives = 339/515 (65.83%), Query Frame = 0

Query: 12  IFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRHGPV 71
           I L   LFL+ Y L      K  NLPP+P  SLP++GH  LL  P++R    +S  HGP+
Sbjct: 6   IVLPLALFLLAYKLFFTSKTKRFNLPPSPPYSLPILGHHNLLKPPVHRLFHRLSKTHGPI 65

Query: 72  VFLRFGSRPVLVVSSPSAAEECLT-KNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHWR 131
             L+FGSR  +V+SS S A +C T +ND++ +NRP  L +K   YN T +  + YGDHWR
Sbjct: 66  FSLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWR 125

Query: 132 SLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVMLR 191
           +LRRI ++E+LS++RL     +R +E+  ++ +L++  N++  + +     DLTFN ++R
Sbjct: 126 NLRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVNKE--IELEPLLSDLTFNNIVR 185

Query: 192 MIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKMMD 251
           M+ GKR+YGD+V + +EEA +F++L A+    +G  + GD++P M    FG S EKK+  
Sbjct: 186 MVTGKRYYGDEVHN-EEEANVFKKLVADINDCSGARHPGDYLPFMKM--FGGSFEKKVKA 245

Query: 252 CHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEPDHYTDESI 311
                D  +Q L+EE +               RD    TM+  LL LQ++EP++YTD +I
Sbjct: 246 LAEAMDEILQRLLEECK---------------RDKDGNTMVNHLLSLQQNEPEYYTDVTI 305

Query: 312 KALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHLPY 371
           K LML ++ AGT+TS  T+EWA+S LLNHPE L+KA++EID+++GQ+RLIDE D+A+LPY
Sbjct: 306 KGLMLGMMIAGTDTSAVTLEWAMSSLLNHPEALEKAKLEIDEKIGQERLIDEPDIANLPY 365

Query: 372 LRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKIWANPTKF 431
           L+ I++ETFR+YP APL+VP   +ED  VGG+ +PRGTM+ VN WAIH DP++W  P KF
Sbjct: 366 LQNIVSETFRLYPAAPLLVPRSPTEDIKVGGYDVPRGTMVMVNAWAIHRDPELWNEPEKF 425

Query: 432 DPDRFDRLESDKYGYGFN-LMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEKL 491
            P+RF+  E    G   + LMPFG+GRR CPG GLG +I+ L LGSLIQCF+W++   + 
Sbjct: 426 KPERFNGGEGGGRGEDVHKLMPFGNGRRSCPGAGLGQKIVTLALGSLIQCFDWQKVNGEA 485

Query: 492 VDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTK 525
           +DMTE   ++M K  PL A C+ RPI++ L +  K
Sbjct: 486 IDMTETPGMAMRKKIPLSALCQSRPIMSKLQAHLK 500

BLAST of MS008718 vs. ExPASy TrEMBL
Match: A0A6J1CYI5 (cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV=1)

HSP 1 Score: 1047.7 bits (2708), Expect = 1.6e-302
Identity = 517/525 (98.48%), Postives = 522/525 (99.43%), Query Frame = 0

Query: 1   VHSIILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRT 60
           +HS ILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRT
Sbjct: 1   MHSKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRT 60

Query: 61  LADISNRHGPVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNL 120
           LADISNRHGPVVFLRFGSRPVLVVSSPS AEECLTKNDVVFANRPRLLISKCFGYNNTNL
Sbjct: 61  LADISNRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNL 120

Query: 121 VWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSF 180
           VWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSF
Sbjct: 121 VWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSF 180

Query: 181 FDLTFNVMLRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGF 240
           FDLTFNVMLRMIAGKRFYGDDVDDADE+AKIFRELQAESTRLTGISNIGDFVPMMTWLGF
Sbjct: 181 FDLTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGF 240

Query: 241 GWSVEKKMMDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQES 300
           GWSVEKKMMDCHYKRDAFMQ+LIEEQRKRSTAETESEIDDSFRDGR+KTMIEVLLELQES
Sbjct: 241 GWSVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQES 300

Query: 301 EPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLI 360
           EPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLI
Sbjct: 301 EPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLI 360

Query: 361 DESDMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHND 420
           DESDMAHLPYLRGII ETFRMYPPAPLMVPHQSSEDC+VGGHHIPRGTMLYVNLWAIHND
Sbjct: 361 DESDMAHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHND 420

Query: 421 PKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCF 480
           PKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCF
Sbjct: 421 PKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCF 480

Query: 481 EWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKT 526
           EWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKT
Sbjct: 481 EWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKT 525

BLAST of MS008718 vs. ExPASy TrEMBL
Match: A0A6J1FJC9 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445994 PE=3 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.5e-231
Identity = 397/518 (76.64%), Postives = 467/518 (90.15%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRH 68
           MGAI +Y PLF VLY+LTE FLH+IRNLPPTPF SLP+IGHL+LL KP+YRTLA+ISNR+
Sbjct: 1   MGAIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 69  GPVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDH 128
           GPVVFLRFGSR VLVVSSPSAAEECL+KNDVVFA+RPRLLISK FGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 129 WRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVM 188
           WR+LRRISTVE+LSTHRLQ+L+ VRL+EV+SLI +LAK ENQ  +  M ++FFDLTFNVM
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKGENQ--VQDMNNAFFDLTFNVM 180

Query: 189 LRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 248
           LRMI GKRFYG+DV+DAD EA  FRELQ++ST+L G +N+GDF+P+M+WLG GW+ EK++
Sbjct: 181 LRMIVGKRFYGEDVEDAD-EAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEI 240

Query: 249 MDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEPDHYTDE 308
           M+CH KRDAFMQ+LI + + R+    E+E D+SFRDGRKKT+IEVLLELQES+PD Y DE
Sbjct: 241 MECHQKRDAFMQNLINQHKNRA---IETEPDNSFRDGRKKTLIEVLLELQESDPDQYNDE 300

Query: 309 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 368
           +I+ALMLVLL AGTETS+NTMEWALSL+LN+PEVL+KAQ+EID +VG DRL+DESDMA++
Sbjct: 301 TIRALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANV 360

Query: 369 PYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 428
           P+LRGIINET RMYPPAPLMVPH+SS+DC+VGG+HIPRGTML+VNLWAIHNDPKIWA+P 
Sbjct: 361 PFLRGIINETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPR 420

Query: 429 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 488
           KFDPDRF ++ES+K   GF LMPFGSGRRGCPGEGLGLR+IGLVLGSLIQCFEWERPGE+
Sbjct: 421 KFDPDRFVKVESEK--LGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEE 480

Query: 489 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKT 526
           LVDMTEG+AVSMPKA+ LQAKC+PRPI+  L  SQTK+
Sbjct: 481 LVDMTEGVAVSMPKARHLQAKCKPRPIVDRLHLSQTKS 510

BLAST of MS008718 vs. ExPASy TrEMBL
Match: A0A1S3BUA3 (LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493587 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.2e-228
Identity = 388/518 (74.90%), Postives = 455/518 (87.84%), Query Frame = 0

Query: 8   AMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNR 67
           AMGA+FLY PLFLVLY+LT+HFLH IRNLPP PFPSLP++GHL+LLNKPIYR L +ISNR
Sbjct: 4   AMGALFLYIPLFLVLYLLTKHFLHNIRNLPPXPFPSLPILGHLHLLNKPIYRALYNISNR 63

Query: 68  HGPVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGD 127
           +GPVVFLR GSR VL+VSS SAAEECLTKND++FANRPRLL+SKCFGYNNTNLVWSSYGD
Sbjct: 64  YGPVVFLRLGSRSVLIVSSASAAEECLTKNDIIFANRPRLLLSKCFGYNNTNLVWSSYGD 123

Query: 128 HWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNV 187
            WR+LRRI TVE+LSTHRL +LS VR EEVRSLI++L K ENQ  ++ M+H FFDL+FN+
Sbjct: 124 IWRNLRRICTVEILSTHRLHMLSVVRFEEVRSLIQRLLKRENQ--VVNMKHVFFDLSFNL 183

Query: 188 MLRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKK 247
           MLRMI GKRFYGDDVDD  EEAK+FRELQA+S+ L+G SN+GDF+P+M+WLGF  ++EK+
Sbjct: 184 MLRMIVGKRFYGDDVDDV-EEAKLFRELQAKSSHLSGKSNMGDFIPLMSWLGFDSTLEKE 243

Query: 248 MMDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEPDHYTD 307
           MMDC   RDA MQSLIE+ R+R       EIDDSFRDGRKKT+IEVLLELQESEP+ Y D
Sbjct: 244 MMDCQNGRDALMQSLIEQHRRRRIV----EIDDSFRDGRKKTLIEVLLELQESEPEQYND 303

Query: 308 ESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAH 367
           E+I+ LML++L AGTETS + MEWALSLLLNHPE+LKKAQ+EID+QVG  R I+ESDMA 
Sbjct: 304 ETIRGLMLLMLVAGTETSGSIMEWALSLLLNHPEILKKAQIEIDNQVGNQRPIEESDMAR 363

Query: 368 LPYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKIWANP 427
           LPYLRGIINET RMYPPAPL+VPH+SS+DC+VGG+H+PRGTMLY+N+WAI NDPKIWA+P
Sbjct: 364 LPYLRGIINETLRMYPPAPLLVPHESSQDCSVGGYHVPRGTMLYINIWAIQNDPKIWAHP 423

Query: 428 TKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGE 487
            +FDPDRF+ +ES+K  Y FNLMPFG GRRGCPGEGLGLR+IGLVLGSLIQCFEWERP E
Sbjct: 424 KEFDPDRFNDVESEK--YKFNLMPFGLGRRGCPGEGLGLRMIGLVLGSLIQCFEWERPTE 483

Query: 488 KLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKT 526
           +LVD+TEGIA++MPKAQ L AKCRPRP+I N+  Q K+
Sbjct: 484 ELVDLTEGIAITMPKAQNLHAKCRPRPMIVNILPQAKS 512

BLAST of MS008718 vs. ExPASy TrEMBL
Match: A0A0A0KZW4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642310 PE=3 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 3.4e-228
Identity = 387/517 (74.85%), Postives = 455/517 (88.01%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRH 68
           MGAIFLY P FLVLY+LT+HFLH IRNLPPTPFPSLP++GHL+LLNKPIYR L +ISNR+
Sbjct: 9   MGAIFLYIPPFLVLYLLTKHFLHNIRNLPPTPFPSLPILGHLHLLNKPIYRALYNISNRY 68

Query: 69  GPVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDH 128
           GPVVFLR GSR VL+VSSPSAAEECLTKND++FANRP LL+SKCFGYNNTNLVWSSYGDH
Sbjct: 69  GPVVFLRLGSRSVLIVSSPSAAEECLTKNDIIFANRPHLLLSKCFGYNNTNLVWSSYGDH 128

Query: 129 WRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVM 188
           WR+LRRI TVE+LSTHRL +L  VR EEVRSLI++L K ENQ  ++ M+H FFDL+FN+M
Sbjct: 129 WRNLRRICTVEILSTHRLHMLYIVRFEEVRSLIQRLVKRENQ--VVNMKHVFFDLSFNLM 188

Query: 189 LRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 248
           LRMI GKRFYGDDVDD  EEAK+FRELQAES+RL+G SN+GDF+P+M+WLGF  ++EK+M
Sbjct: 189 LRMIVGKRFYGDDVDDV-EEAKLFRELQAESSRLSGKSNMGDFIPLMSWLGFDSTLEKEM 248

Query: 249 MDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEPDHYTDE 308
           +DC   RDA MQSLIE+ R+R  A    EID SFRDGRKKT+IEVLLELQESEP+ YTDE
Sbjct: 249 IDCQNGRDALMQSLIEQHRRRRIA----EIDYSFRDGRKKTLIEVLLELQESEPEQYTDE 308

Query: 309 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 368
           +I+ALML++L AGTETS + MEWALSLLLNHPE+LKKAQ EID+QVG  RL++ESDMA L
Sbjct: 309 TIRALMLLMLVAGTETSGSIMEWALSLLLNHPEILKKAQTEIDNQVGHKRLMEESDMARL 368

Query: 369 PYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 428
           PYLRGIINET RMYPPAPL+VPH+SS+DC+VGG+H+PRGTMLY+N+WAI NDPK W +P 
Sbjct: 369 PYLRGIINETLRMYPPAPLLVPHESSQDCSVGGYHVPRGTMLYINIWAIQNDPKFWTHPR 428

Query: 429 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 488
           KFDP+RF+ +ES+   Y FNLM FG GRRGCPGEGLGLR+IGLVLGSLIQCFEWERP E+
Sbjct: 429 KFDPERFNDVESE--NYKFNLMAFGLGRRGCPGEGLGLRMIGLVLGSLIQCFEWERPTEE 488

Query: 489 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKT 526
           LVD+TEGIA++MPKAQ L AKCRPRP++ ++ SQTK+
Sbjct: 489 LVDLTEGIAITMPKAQNLHAKCRPRPMVVDILSQTKS 516

BLAST of MS008718 vs. ExPASy TrEMBL
Match: A0A6J1J228 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119 PE=3 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.3e-227
Identity = 393/518 (75.87%), Postives = 464/518 (89.58%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRH 68
           MGAI LY PLFLVLY+LTE F+HKIRNLPPTPF SLP+IGHL+LL KP+YRTLA+ISNR+
Sbjct: 1   MGAIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 69  GPVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDH 128
           GPVVFLRFGSR VLVVSSPSAAEECL+KNDVVFA+RPRLLISK FGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 129 WRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVM 188
           WR+LRRISTVE+LSTHRLQ+L+ VRL+EV+SLI +LA+ ENQ  +  M ++FFDLTFNVM
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARGENQ--VQDMNNAFFDLTFNVM 180

Query: 189 LRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 248
           LRMI GKRFYG+DV+DAD EA  FRELQ++ST+L G +N+GDF+P+M+WLG G + EK++
Sbjct: 181 LRMIVGKRFYGEDVEDAD-EAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEI 240

Query: 249 MDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEPDHYTDE 308
           M+CH KRDAFMQ+LI + + R+    E+E  + FRDGRKKT+IEVLLELQES+PD Y DE
Sbjct: 241 MECHQKRDAFMQNLINQHKNRA---IETEPHNLFRDGRKKTLIEVLLELQESDPDQYNDE 300

Query: 309 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 368
           +I+ALMLVLL AGTETS+NTMEWALSL+LN+PEVL+KAQ+EID +VG DRL+DESDMA++
Sbjct: 301 TIRALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANV 360

Query: 369 PYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 428
           P+LRGIINET RMYPPAPLMVPH+SS+DC+VGG+HIPRGTML+VNLWAIHNDPKIWA+P 
Sbjct: 361 PFLRGIINETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPR 420

Query: 429 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 488
           KFDPDRF +++S+K   GF L+PFGSGRRGCPGEGLGLR+IGLVLGSLIQCFEWERPGE 
Sbjct: 421 KFDPDRFVKVDSEK--LGFKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGED 480

Query: 489 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKT 526
           LVDMTEG+AVSMPKA+ LQAKC+PRPI+  L  SQTK+
Sbjct: 481 LVDMTEGVAVSMPKARHLQAKCKPRPIVDRLHLSQTKS 510

BLAST of MS008718 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 471.9 bits (1213), Expect = 6.9e-133
Identity = 243/493 (49.29%), Postives = 333/493 (67.55%), Query Frame = 0

Query: 35  NLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRHG--PVVFLRFGSRPVLVVSSPSAAEE 94
           NLPP+P  +LP+IGHL LL  P++R    +S   G  P++ LR G+R V VVSS S AEE
Sbjct: 29  NLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIISLRLGNRLVFVVSSHSLAEE 88

Query: 95  CLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHWRSLRRISTVEVLSTHRLQILSTV 154
           C TKNDVV ANR   L SK   Y  T +V +SYGDHWR+LRRI  VE+ S HRL   S++
Sbjct: 89  CFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLRRIGAVEIFSAHRLNSFSSI 148

Query: 155 RLEEVRSLIRKLAKSENQD-KILTMRHSFFDLTFNVMLRMIAGKRFYGDDVDDADEEAKI 214
           R +E+  LI  L+++ + +   + M+  F +LTFN ++RM+AGK +YGD  +D D EAK 
Sbjct: 149 RRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIRMLAGKCYYGDGAED-DPEAKR 208

Query: 215 FRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKMMDCHYKRDAFMQSLIEEQRKRST 274
            REL AE     G  N  D++P++TW+      EK++     + D F+Q L++E+R+   
Sbjct: 209 VRELIAEGMGCFGAGNTADYLPILTWI---TGSEKRIKKIASRLDEFLQGLVDERREG-- 268

Query: 275 AETESEIDDSFRDGRKKTMIEVLLELQESEPDHYTDESIKALMLVLLTAGTETSVNTMEW 334
                      ++ R+ TM++ LL LQE++P++YTD  IK +ML L+ AGT+TS  T+EW
Sbjct: 269 -----------KEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTDTSAVTLEW 328

Query: 335 ALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHLPYLRGIINETFRMYPPAPLMVPH 394
            LS LLNHP++L KA+ EID++VG +RL++ESD++HLPYL+ I++E+ R+YP +PL+VPH
Sbjct: 329 TLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASPLLVPH 388

Query: 395 QSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKIWANPTKFDPDRFDRLESDKYGYGFNLMP 454
            +SEDC VGG+H+PRGTML  N WAIH DPKIW +PT F P+RF     +K G    L+ 
Sbjct: 389 VASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERF-----EKEGEAQKLLG 448

Query: 455 FGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEKLVDMTE-GIAVSMPKAQPLQAKC 514
           FG GRR CPG GL  R+  L +GSLIQCFEWER GE+ VDMTE G  V MPKA PL A C
Sbjct: 449 FGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLVAMC 499

Query: 515 RPRPIIANLPSQT 524
           + RP++  + +++
Sbjct: 509 KARPVVGKILNES 499

BLAST of MS008718 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 464.9 bits (1195), Expect = 8.4e-131
Identity = 243/508 (47.83%), Postives = 343/508 (67.52%), Query Frame = 0

Query: 18  LFLVLYILTEHFLHKIR---NLPPTPFPSLPLIGHLYLLNKPIYRTLADISN--RHGPVV 77
           LF+VL ++  + + K++   NLPP+P  SLP+IGHL LL  PI+RT   +S    + P+ 
Sbjct: 11  LFVVLSLI--YLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIF 70

Query: 78  FLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHWRSL 137
            LR G+R V V SS S AEEC TKNDVV ANRP  +++K   Y+ T ++ +SYGDHWR+L
Sbjct: 71  SLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNL 130

Query: 138 RRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKI-LTMRHSFFDLTFNVMLRM 197
           RRI +VE+ S HRL    ++R +E+R L+ +L+++ +Q+ + + M+    DLTFN +LRM
Sbjct: 131 RRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRM 190

Query: 198 IAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKMMDC 257
           +AGKR+YGD V+D D EAK  R+L A+     G  N  D++P++  +      E ++   
Sbjct: 191 VAGKRYYGDGVED-DPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVS---DYETRVKKL 250

Query: 258 HYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEPDHYTDESIK 317
             + D F+Q L++E+R+                 +  TMI+ LL LQES+PD++TD  IK
Sbjct: 251 AGRLDEFLQGLVDEKREAKE--------------KGNTMIDHLLTLQESQPDYFTDRIIK 310

Query: 318 ALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHLPYL 377
             ML L+ AGT+TS  T+EWALS +LNHP+VL KA+ EID ++G DRL+DESD+++LPYL
Sbjct: 311 GNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYL 370

Query: 378 RGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKIWANPTKFD 437
           + I++ET R+YP AP+++PH +SEDC V G+ +PRGT+L  N+WAIH DP++W +P  F 
Sbjct: 371 QNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFK 430

Query: 438 PDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEKLVD 497
           P+RF     +K G    LMPFG GRR CPG GL  R+I L LGSLIQC EWE+ GE+ VD
Sbjct: 431 PERF-----EKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VD 490

Query: 498 MTEGIAVSMPKAQPLQAKCRPRPIIANL 520
           M+EG  V+MPKA+PL+A CR RP +  +
Sbjct: 491 MSEGKGVTMPKAKPLEAMCRARPSVVKI 492

BLAST of MS008718 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 458.8 bits (1179), Expect = 6.0e-129
Identity = 239/517 (46.23%), Postives = 340/517 (65.76%), Query Frame = 0

Query: 6   LAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADIS 65
           + A+  IF +  + L L  L      K+ NLPP+P  +LP+IGHL LL  P++R    +S
Sbjct: 1   MEALMLIFTFCFIVLSLIFLIGRIKRKL-NLPPSPAWALPVIGHLRLLKPPLHRVFLSVS 60

Query: 66  NRHG--PVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWS 125
              G  P++ LR G+R + VVSS S AEEC TKNDV+ ANR   + +K   Y N+ +V +
Sbjct: 61  QSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSA 120

Query: 126 SYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQD-KILTMRHSFFD 185
           SY +HWR+LRRI  +E+ S HRL   S++R +E+R LI +L ++ +     + M+  F D
Sbjct: 121 SYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSD 180

Query: 186 LTFNVMLRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGW 245
           LTFN ++RM+AGK +YGD  +D D EAK  R L AE+   +G  N  D++P++TW+ +  
Sbjct: 181 LTFNNIIRMLAGKCYYGDGKED-DPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYSE 240

Query: 246 SVEKKMMDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEP 305
           +  KK+     + D F+Q L++E+R+              ++ ++ TM++ LL LQE++P
Sbjct: 241 TRIKKLAG---RLDEFLQGLVDEKREG-------------KEKKENTMVDHLLCLQETQP 300

Query: 306 DHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDE 365
           ++Y D  IK  ML L+  GT+T+  T+EWALS LLN+PEVL KA+ EID  +G DRL++E
Sbjct: 301 EYYMDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEE 360

Query: 366 SDMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPK 425
           SD+ +LPYL+ I++ET R+YP AP+++PH +S+DC VGG+ +PRGTML  N WAIH DP 
Sbjct: 361 SDIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPL 420

Query: 426 IWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEW 485
           +W +PT F P+RF     +K G    LMPFG GRR CPG GL  R++ L LGSLIQCFEW
Sbjct: 421 LWDDPTSFKPERF-----EKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEW 480

Query: 486 ERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANL 520
           ER GE+ VDMTEG  ++MPKA+PL+A CR R  +  +
Sbjct: 481 ERIGEEEVDMTEGPGLTMPKARPLEAMCRARDFVGKI 494

BLAST of MS008718 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 456.1 bits (1172), Expect = 3.9e-128
Identity = 244/511 (47.75%), Postives = 332/511 (64.97%), Query Frame = 0

Query: 12  IFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRHG-- 71
           I  +  LF +   L      K+ NLPP+P  SLP+IGHL+LL  P++RT   +S   G  
Sbjct: 8   ILSFLSLFTLAIFLLTRSKRKL-NLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNA 67

Query: 72  PVVFLRFGSRPVLVVSSPSAAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHW 131
           PV  LR G+R V V+SS S AEEC TKNDVV ANRP+  ISK  GYN T L+ +SYGDHW
Sbjct: 68  PVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHW 127

Query: 132 RSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKI-LTMRHSFFDLTFNVM 191
           R+LRRI+ VE+ STHRL     +R +E+R LI  L++      + + M+    +L  N  
Sbjct: 128 RNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTT 187

Query: 192 LRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 251
           +RM+AGKR++G+D DD    AK+ + L +E+    G  N  D++ ++ W+    S EK++
Sbjct: 188 IRMLAGKRYFGEDNDD----AKLVKNLVSEAVTSAGAGNPIDYLSILRWVS---SYEKRI 247

Query: 252 MDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEPDHYTDE 311
            +   + D F+Q L++E+R    AE E          + +TMI+ LL LQ+ +PD+YTD 
Sbjct: 248 KNLGNRFDTFLQKLVDEKR----AEKE----------KGETMIDHLLALQDIQPDYYTDV 307

Query: 312 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 371
            IK ++L L+ AGT+TS  T+EWA+S LLNHPE+LKKA++EID++VG DRL+DESD+ +L
Sbjct: 308 IIKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNL 367

Query: 372 PYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 431
            YL+ I+ ET RMYP  PL++PH SSEDC VGG+ IP GTM+  N WA+H DP++W +P 
Sbjct: 368 SYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPE 427

Query: 432 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 491
            F P+RF     +K G    L+ FG GRR CPG GL  R+I   LGSL+QCFEWER GE 
Sbjct: 428 IFKPERF-----EKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGED 487

Query: 492 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANL 520
            VDMTE    ++PKA PL+A C+ R I+  L
Sbjct: 488 FVDMTEDKGATLPKAIPLRAMCKARSIVDKL 491

BLAST of MS008718 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 455.3 bits (1170), Expect = 6.7e-128
Identity = 246/518 (47.49%), Postives = 337/518 (65.06%), Query Frame = 0

Query: 12  IFLYFPLFLVLYILTEHFLH-KIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADIS----- 71
           + LY    L+  I++  FL  K +NLPP+P   LP+IGHL LL  PI+RTL   S     
Sbjct: 8   VVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 67

Query: 72  NRHGPVVFLRFGSRPVLVVSSPS-AAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSS 131
           N  G V+ LR GSR V VVSS   AAEEC  KNDVV ANRP+++I K  GYNNTN++ + 
Sbjct: 68  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 127

Query: 132 YGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDK-ILTMRHSFFDL 191
           YGDHWR+LRR+ T+E+ STHRL     VR +EVR LI +L++     K ++ ++    DL
Sbjct: 128 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDL 187

Query: 192 TFNVMLRMIAGKRFYGDDVDDADEEAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWS 251
           TFN ++RM+ GKR+YG++  D +EEAK  R+L A+    T   N  D+VP++       S
Sbjct: 188 TFNNIMRMMTGKRYYGEETTD-EEEAKRVRKLVADVGANTSSGNAVDYVPILRLFS---S 247

Query: 252 VEKKMMDCHYKRDAFMQSLIEEQRKRSTAETESEIDDSFRDGRKKTMIEVLLELQESEPD 311
            E ++     + D F+Q LI+++R +    T              TMI+ LL LQ+S+ +
Sbjct: 248 YENRVKKLGEETDKFLQGLIDDKRGQQETGT--------------TMIDHLLVLQKSDIE 307

Query: 312 HYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDES 371
           +YTD+ IK ++L+++ AGT TS  T+EWALS LLNHP+V+ KA+ EID++VG DRLI+E+
Sbjct: 308 YYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEA 367

Query: 372 DMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCTVGGHHIPRGTMLYVNLWAIHNDPKI 431
           D++ LPYL+ I+ ET R++P  PL+VPH +SEDC +G + +PRGT L VN WAIH DP  
Sbjct: 368 DLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNT 427

Query: 432 WANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWE 491
           W +P  F P+RF++ E  +      L+ FG GRR CPG GL  RI+GL LGSLIQCFEWE
Sbjct: 428 WDDPDSFKPERFEKEEEAQ-----KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWE 487

Query: 492 RPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPS 522
           R G   VDM EG+  ++PKA PL+A C+ RP +  + S
Sbjct: 488 RVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKIIS 502