MS008658 (gene) Bitter gourd (TR) v1

Overview
NameMS008658
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPhosphatase 2C family protein
Locationscaffold4: 2891355 .. 2893062 (+)
RNA-Seq ExpressionMS008658
SyntenyMS008658
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAATCTGCATATCCACTGCATCTTCTCAGATTCGCCATGAAACCGAAGGCAGCGTTATTTGCAGAAACGAGCTCATCTACGAACAAAACGGCTCCATCTGTGGGATTCAGAAGCTCGATTCCATCTGTTCCAAGCAAGGAGCCAAAGGAATCAACCAAGACGCAGCCTTTTTCTACCAGGTAATTAATTTTAATTCCCTCTTCGCGTTTCGCTTCCATTTTCACACACTGGTCTCTGTTCTGAACTCTGTTTTCGCCAGAACTACGGAGCAGAGAACACGGCCCTGCTCGGAGTTTTTGATGGGCACGGGAAAAACGGCCACGTCGTCAGCAACATGGTAAAAAATCGCCTCCCTTTGCTGTTGATTGGGCAGATAAATGAAAGAAATCGCGAAGAGCAGTTTGTTACTGCGTTTAAGGTGATGGACAAGGAGATTAAGCTTCATGAGAATCTGGATTGCTGGTGCAGTGGATCCACGGCTGTAATTGCAGTAAAAGAGGGAGAAGATTTGGTAATTGGTAATTTGGGGGATTCGAGGGCGGTGTTGGGGAGAATGGGCGAGGGCGGAATCGAAGCCGTTCAGTTAACGACTGATCTGAAGCCTGGTTTACCCGGTAAGTAAGATCCCGTCGTATAATTAAATGTACCCAACTCATCTAAGTTTTAGCAAATAATGTGAAAATTGTGGGTGCAGATGAAGGGGAGAGAATAAGGAGGAATAATGGACGGGTGTTGGCGCTGAAAGAAGAGCCGCATATACAGAGGGTGTGGCTGCCGAATGAAGACGCGCCGGGGCTGGCCATGTCCAGAGCTTTTGGAGACTTTGCTCTTAAAAACCATGGCATTATTGCTCTCCCCGACGTCTCCCACCGCCGTCTGACTTCCGACGACCGTTTCATCGTACTCGCCACTGATGGGGTAATCTAAACCTCAACTTCAACTCTAAAAACATGTTTAACTCATGGATTAATCTATTTTAATTTTGTGTTTAACCGATCCAACGTGTTTGATAACTTTTAATTGAAAATACATAAGACTTGACGGATGAAACGTATTTATATGTGACTACTATGGAAAATTTGCCAGTTTGTTGTTTTTATTTTAGTTCTCTATTTTTACATTTATCTTCCTAATTATGATCCAAATATTTGATAAAATTTAGAAAATTGACCGTTATATATTATAGCTTCTCCTTTTCTTTTATGCCCCCTTAGTTGTCATTTCTTAGTATTTGAATAGGTTTGATACAATATTGAGTTTCGTGCCTTTTAATTTTGTGCATATTGTGTTATAAAATACTCATTTAACTTATTAGAATACACGATTGAAATTTGTGAAAGTAATTAGACATTTTGAAGTTAAATAAATAAATACTTTGATTTTGAAAAATTAGAGGCAAAATTAACACATAATATAATTTGTTTGCTCCATTATTAGATGTTAGATTGGATGATGTTGGGCAATTTTTAGGAAAAGATAATTAGATTTACCTTAATTCATGTGTTGGAATTTTGGGTATTTATTTTCCAGATTTGGGACGTGCTAAGCAACGACCAAGTGGCGTCCATCGTGTGGGAGGCGGAGAACGAAGAAGCGGCGGCGAAAGCGGTGGTGGAGGCGGCGGCGGAGGCTTGGAAACGCTACCCTTCTTCAAAACTAGACGACTGCACCGTCGTTTGCCTGTTTTTACAGAACCAAGCCGAG

mRNA sequence

ATGGGAATCTGCATATCCACTGCATCTTCTCAGATTCGCCATGAAACCGAAGGCAGCGTTATTTGCAGAAACGAGCTCATCTACGAACAAAACGGCTCCATCTGTGGGATTCAGAAGCTCGATTCCATCTGTTCCAAGCAAGGAGCCAAAGGAATCAACCAAGACGCAGCCTTTTTCTACCAGAACTACGGAGCAGAGAACACGGCCCTGCTCGGAGTTTTTGATGGGCACGGGAAAAACGGCCACGTCGTCAGCAACATGGTAAAAAATCGCCTCCCTTTGCTGTTGATTGGGCAGATAAATGAAAGAAATCGCGAAGAGCAGTTTGTTACTGCGTTTAAGGTGATGGACAAGGAGATTAAGCTTCATGAGAATCTGGATTGCTGGTGCAGTGGATCCACGGCTGTAATTGCAGTAAAAGAGGGAGAAGATTTGGTAATTGGTAATTTGGGGGATTCGAGGGCGGTGTTGGGGAGAATGGGCGAGGGCGGAATCGAAGCCGTTCAGTTAACGACTGATCTGAAGCCTGGTTTACCCGATGAAGGGGAGAGAATAAGGAGGAATAATGGACGGGTGTTGGCGCTGAAAGAAGAGCCGCATATACAGAGGGTGTGGCTGCCGAATGAAGACGCGCCGGGGCTGGCCATGTCCAGAGCTTTTGGAGACTTTGCTCTTAAAAACCATGGCATTATTGCTCTCCCCGACGTCTCCCACCGCCGTCTGACTTCCGACGACCGTTTCATCGTACTCGCCACTGATGGGATTTGGGACGTGCTAAGCAACGACCAAGTGGCGTCCATCGTGTGGGAGGCGGAGAACGAAGAAGCGGCGGCGAAAGCGGTGGTGGAGGCGGCGGCGGAGGCTTGGAAACGCTACCCTTCTTCAAAACTAGACGACTGCACCGTCGTTTGCCTGTTTTTACAGAACCAAGCCGAG

Coding sequence (CDS)

ATGGGAATCTGCATATCCACTGCATCTTCTCAGATTCGCCATGAAACCGAAGGCAGCGTTATTTGCAGAAACGAGCTCATCTACGAACAAAACGGCTCCATCTGTGGGATTCAGAAGCTCGATTCCATCTGTTCCAAGCAAGGAGCCAAAGGAATCAACCAAGACGCAGCCTTTTTCTACCAGAACTACGGAGCAGAGAACACGGCCCTGCTCGGAGTTTTTGATGGGCACGGGAAAAACGGCCACGTCGTCAGCAACATGGTAAAAAATCGCCTCCCTTTGCTGTTGATTGGGCAGATAAATGAAAGAAATCGCGAAGAGCAGTTTGTTACTGCGTTTAAGGTGATGGACAAGGAGATTAAGCTTCATGAGAATCTGGATTGCTGGTGCAGTGGATCCACGGCTGTAATTGCAGTAAAAGAGGGAGAAGATTTGGTAATTGGTAATTTGGGGGATTCGAGGGCGGTGTTGGGGAGAATGGGCGAGGGCGGAATCGAAGCCGTTCAGTTAACGACTGATCTGAAGCCTGGTTTACCCGATGAAGGGGAGAGAATAAGGAGGAATAATGGACGGGTGTTGGCGCTGAAAGAAGAGCCGCATATACAGAGGGTGTGGCTGCCGAATGAAGACGCGCCGGGGCTGGCCATGTCCAGAGCTTTTGGAGACTTTGCTCTTAAAAACCATGGCATTATTGCTCTCCCCGACGTCTCCCACCGCCGTCTGACTTCCGACGACCGTTTCATCGTACTCGCCACTGATGGGATTTGGGACGTGCTAAGCAACGACCAAGTGGCGTCCATCGTGTGGGAGGCGGAGAACGAAGAAGCGGCGGCGAAAGCGGTGGTGGAGGCGGCGGCGGAGGCTTGGAAACGCTACCCTTCTTCAAAACTAGACGACTGCACCGTCGTTTGCCTGTTTTTACAGAACCAAGCCGAG

Protein sequence

MGICISTASSQIRHETEGSVICRNELIYEQNGSICGIQKLDSICSKQGAKGINQDAAFFYQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNREEQFVTAFKVMDKEIKLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEAVQLTTDLKPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWKRYPSSKLDDCTVVCLFLQNQAE
Homology
BLAST of MS008658 vs. NCBI nr
Match: XP_022154915.1 (probable protein phosphatase 2C 72 [Momordica charantia])

HSP 1 Score: 621.3 bits (1601), Expect = 4.5e-174
Identity = 308/312 (98.72%), Postives = 308/312 (98.72%), Query Frame = 0

Query: 1   MGICISTASSQIRHETEGSVICRNELIYEQNGSICGIQKLDSICSKQGAKGINQDAAFFY 60
           MGICISTASSQIRHETEGSVICRNELIYEQNGSICGIQKLD ICSKQGAKGINQDAAFFY
Sbjct: 1   MGICISTASSQIRHETEGSVICRNELIYEQNGSICGIQKLDYICSKQGAKGINQDAAFFY 60

Query: 61  QNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNREEQFVTAFKVMDKEI 120
           QNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNREEQFVTAFKVMDKEI
Sbjct: 61  QNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNREEQFVTAFKVMDKEI 120

Query: 121 KLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEAVQLTTDLKPGLPD 180
           KLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEAVQLTTDLKPGLPD
Sbjct: 121 KLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEAVQLTTDLKPGLPD 180

Query: 181 EGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRR 240
           EGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRR
Sbjct: 181 EGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRR 240

Query: 241 LTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWKRYPSSKLDDC 300
           LTSDDRFIVLATDGIWDVLSNDQV SIVWEAENEEAAAKA VEAAAEAWKRYPSSKLDDC
Sbjct: 241 LTSDDRFIVLATDGIWDVLSNDQVVSIVWEAENEEAAAKAAVEAAAEAWKRYPSSKLDDC 300

Query: 301 TVVCLFLQNQAE 313
           TVV LFLQNQAE
Sbjct: 301 TVVWLFLQNQAE 312

BLAST of MS008658 vs. NCBI nr
Match: XP_038899305.1 (probable protein phosphatase 2C 72 [Benincasa hispida])

HSP 1 Score: 464.2 bits (1193), Expect = 9.2e-127
Identity = 241/321 (75.08%), Postives = 269/321 (83.80%), Query Frame = 0

Query: 1   MGICISTASSQIRHETE--GSVICRNELIYEQNGSICGIQKLDSICSKQGAKGINQDAAF 60
           MGICIS+ASSQIRHET   GS+   +   Y    S+CGIQKL+SICSKQG+KG+NQDAAF
Sbjct: 1   MGICISSASSQIRHETNGVGSIFEEDYKSY----SVCGIQKLESICSKQGSKGLNQDAAF 60

Query: 61  FYQNY-GAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNR----------EE 120
            YQ + G E + L+GVFDGHG+NGH+VSN+VKNRLP LL+GQI  ++           +E
Sbjct: 61  SYQEFGGVEESDLVGVFDGHGQNGHIVSNIVKNRLPSLLLGQITAKSSLMQSKSFDKWKE 120

Query: 121 QFVTAFKVMDKEIKLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEA 180
            F T+FKVMDKEIKL ENLDC CSGSTAV+ VK+G++LVIGNLGDSRAV+GRM + GIEA
Sbjct: 121 AFETSFKVMDKEIKLQENLDCSCSGSTAVVLVKQGDNLVIGNLGDSRAVMGRMRDDGIEA 180

Query: 181 VQLTTDLKPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKN 240
           VQLTTDLKPGL  EGERIR   GRVLALKEE HIQRVWLPNEDAPGLAMSRAFGDFALK+
Sbjct: 181 VQLTTDLKPGLASEGERIRSCKGRVLALKEEAHIQRVWLPNEDAPGLAMSRAFGDFALKD 240

Query: 241 HGIIALPDVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAE 300
           +GIIA+PDVS RRLTS DRFIVLATDGIWDVLSNDQVASIVW AE+EEAAAKAVVEAAAE
Sbjct: 241 YGIIAIPDVSFRRLTSHDRFIVLATDGIWDVLSNDQVASIVWAAESEEAAAKAVVEAAAE 300

Query: 301 AWKRYPSSKLDDCTVVCLFLQ 309
           AWKRYPSSK DDCT VC FLQ
Sbjct: 301 AWKRYPSSKHDDCTAVCHFLQ 317

BLAST of MS008658 vs. NCBI nr
Match: XP_023524315.1 (probable protein phosphatase 2C 72 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 459.1 bits (1180), Expect = 2.9e-125
Identity = 236/318 (74.21%), Postives = 262/318 (82.39%), Query Frame = 0

Query: 1   MGICISTASSQIRHETEGSVIC-RNELIYEQNGSICGIQKLDSICSKQGAKGINQDAAFF 60
           MGICIS ASSQIRHET+ + IC  NEL +E+       Q+ DS+CSKQG KGINQDAAF 
Sbjct: 1   MGICISAASSQIRHETKVTAICAANELAFEE-------QRSDSVCSKQGNKGINQDAAFV 60

Query: 61  YQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNRE-----EQFVTAFK 120
           YQ YG +  AL GVFDGHG NGH VSNM K+RLP LL+  +++   +     E  VTAFK
Sbjct: 61  YQEYGEQGRALFGVFDGHGTNGHTVSNMAKSRLPPLLLSAVSKAETDFVGWKEAMVTAFK 120

Query: 121 VMDKEIKLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEAVQLTTDL 180
           VMDKEIKL ENLDC  SG+TAVI +K+GEDLVI NLGDSRAV+GR+ E GIEAVQLTTDL
Sbjct: 121 VMDKEIKLQENLDCSFSGTTAVIILKQGEDLVIANLGDSRAVMGRVTENGIEAVQLTTDL 180

Query: 181 KPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALP 240
           KPGLPDE ERIR+ NGRVLAL+EEPHIQRVWLP EDAPGLAMSRAFGDFALK HGIIALP
Sbjct: 181 KPGLPDEAERIRKCNGRVLALQEEPHIQRVWLPKEDAPGLAMSRAFGDFALKEHGIIALP 240

Query: 241 DVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWKRYPS 300
           DVS+RRL  DD  IVLATDGIWDVLSND+VASIVW+AE+EEAAAKAVVEAA EAWKRYP+
Sbjct: 241 DVSYRRLAPDDHSIVLATDGIWDVLSNDEVASIVWKAESEEAAAKAVVEAATEAWKRYPA 300

Query: 301 SKLDDCTVVCLFLQNQAE 313
           SKLDD TVVC FLQNQ +
Sbjct: 301 SKLDDRTVVCHFLQNQRQ 311

BLAST of MS008658 vs. NCBI nr
Match: KAG6608387.1 (putative protein phosphatase 2C 72, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 458.0 bits (1177), Expect = 6.6e-125
Identity = 236/318 (74.21%), Postives = 262/318 (82.39%), Query Frame = 0

Query: 1   MGICISTASSQIRHETEGSVIC-RNELIYEQNGSICGIQKLDSICSKQGAKGINQDAAFF 60
           MGICIS ASSQIRHET+ + IC  NEL +E+       Q+ DS+CSKQG KGINQDAAF 
Sbjct: 1   MGICISAASSQIRHETKVTAICAANELAFEE-------QRSDSVCSKQGNKGINQDAAFV 60

Query: 61  YQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNRE-----EQFVTAFK 120
           YQ YG +  AL GVFDGHG NGH VSNM KNRLP LL+  +++   +     E  VTAFK
Sbjct: 61  YQEYGEQGRALFGVFDGHGTNGHTVSNMAKNRLPALLLSAVSKAETDFVGWKEAMVTAFK 120

Query: 121 VMDKEIKLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEAVQLTTDL 180
           VMDKEIKL ENLDC  SG+TAVI +K+GEDLVI NLGDSRAV+GR+ E GIEAVQLTTDL
Sbjct: 121 VMDKEIKLQENLDCSFSGTTAVIILKQGEDLVIANLGDSRAVMGRVTENGIEAVQLTTDL 180

Query: 181 KPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALP 240
           KPGLPDE ERIR+ NGRVLAL+EEPHIQRVWLP EDAPGLAMSRAFGDFALK HGIIALP
Sbjct: 181 KPGLPDEAERIRKCNGRVLALQEEPHIQRVWLPKEDAPGLAMSRAFGDFALKEHGIIALP 240

Query: 241 DVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWKRYPS 300
           DVS+RRL  DD FIVLATDGIWDVLSND+VASIV +AE+EEAAAKAVVEAA EAWKRYP+
Sbjct: 241 DVSYRRLAPDDHFIVLATDGIWDVLSNDEVASIVSKAESEEAAAKAVVEAATEAWKRYPA 300

Query: 301 SKLDDCTVVCLFLQNQAE 313
           SKLDD TVVC FLQN+ +
Sbjct: 301 SKLDDRTVVCHFLQNKRQ 311

BLAST of MS008658 vs. NCBI nr
Match: XP_022941073.1 (probable protein phosphatase 2C 72 [Cucurbita moschata])

HSP 1 Score: 454.5 bits (1168), Expect = 7.3e-124
Identity = 235/318 (73.90%), Postives = 262/318 (82.39%), Query Frame = 0

Query: 1   MGICISTASSQIRHETEGSVIC-RNELIYEQNGSICGIQKLDSICSKQGAKGINQDAAFF 60
           MGICIS ASSQIRHET+ + IC  NEL +E+       Q+ DS+CSKQG KGINQDAAF 
Sbjct: 1   MGICISAASSQIRHETKVTAICAANELAFEE-------QRSDSVCSKQGNKGINQDAAFV 60

Query: 61  YQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNRE-----EQFVTAFK 120
           YQ YG +  AL GVFDGHG NGH VSNM K+RLP LL+  +++   +     E  VTAFK
Sbjct: 61  YQEYGEQGRALFGVFDGHGTNGHTVSNMAKSRLPPLLLSAMSKAETDFVGWKEAMVTAFK 120

Query: 121 VMDKEIKLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEAVQLTTDL 180
           VMDKEIKL ENLDC  SG+TAVI +K+GEDLVI NLGDSRAV+GR+ E GIEAVQLTTDL
Sbjct: 121 VMDKEIKLQENLDCSFSGTTAVIILKQGEDLVIANLGDSRAVMGRVTENGIEAVQLTTDL 180

Query: 181 KPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALP 240
           KPGLPDE ERIR+ NGRVLAL+EEPHIQRVWLP EDAPGLAMSRAFGDFALK HGIIALP
Sbjct: 181 KPGLPDEAERIRKCNGRVLALQEEPHIQRVWLPKEDAPGLAMSRAFGDFALKEHGIIALP 240

Query: 241 DVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWKRYPS 300
           DVS+RRL  DD FIVLATDGIWDVLSND+VASIV +AE+EEAAAKAVVEAA EAWKRYP+
Sbjct: 241 DVSYRRLAPDDHFIVLATDGIWDVLSNDEVASIVSKAESEEAAAKAVVEAATEAWKRYPA 300

Query: 301 SKLDDCTVVCLFLQNQAE 313
           SKLDD TVVC FLQN+ +
Sbjct: 301 SKLDDRTVVCHFLQNKRQ 311

BLAST of MS008658 vs. ExPASy Swiss-Prot
Match: Q9XGZ9 (Probable protein phosphatase 2C 72 OS=Arabidopsis thaliana OX=3702 GN=At5g26010 PE=2 SV=2)

HSP 1 Score: 326.6 bits (836), Expect = 3.0e-88
Identity = 177/333 (53.15%), Postives = 230/333 (69.07%), Query Frame = 0

Query: 1   MGICISTASSQIR-HET----EGSVICRNELI-YEQNGSICGIQKLDSICSKQGAKGINQ 60
           MG C S  SSQ   HE     +G+V+C  E    +Q+     + +L S+CS QG K +NQ
Sbjct: 1   MGHCFSLPSSQSEIHEDNEHGDGNVVCYGEEFGLDQD---LPVHRLGSVCSIQGTKVLNQ 60

Query: 61  DAAFFYQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNREEQF----- 120
           D A  YQ YG  +T L GVFDGHGKNGH+VS MV+NRLP +L+    E N+E        
Sbjct: 61  DHAVLYQGYGTRDTELCGVFDGHGKNGHMVSKMVRNRLPSVLLALKEELNQESNVCEEEA 120

Query: 121 -------VTAFKVMDKEIKLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGE 180
                   TAF+++D+E+ L +  +C  SGST V+A+ +G+DLVI NLGDSRAVLG M E
Sbjct: 121 SKWEKACFTAFRLIDRELNL-QVFNCSFSGSTGVVAITQGDDLVIANLGDSRAVLGTMTE 180

Query: 181 GG-IEAVQLTTDLKPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFG 240
            G I+AVQLT+DL P +P E ERIR   GRV A+K EP  QRVWLPN++ PGLAMSRAFG
Sbjct: 181 DGEIKAVQLTSDLTPDVPSEAERIRMCKGRVFAMKTEPSSQRVWLPNQNIPGLAMSRAFG 240

Query: 241 DFALKNHGIIALPDVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEA-AAKA 300
           DF LK+HG+IA+P++S  R+TS D+F+VLATDG+WD+LSND+V S++W +  ++A AAK 
Sbjct: 241 DFRLKDHGVIAVPEISQHRITSKDQFLVLATDGVWDMLSNDEVVSLIWSSGKKQASAAKM 300

Query: 301 VVEAAAEAW-KRYPSSKLDDCTVVCLFLQNQAE 313
           V EAA  AW KR   +K+DD TV+CLFLQN+ +
Sbjct: 301 VAEAAEAAWKKRLKYTKVDDITVICLFLQNKEQ 329

BLAST of MS008658 vs. ExPASy Swiss-Prot
Match: Q8H4S6 (Probable protein phosphatase 2C 64 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0566200 PE=2 SV=2)

HSP 1 Score: 292.0 bits (746), Expect = 8.2e-78
Identity = 143/288 (49.65%), Postives = 200/288 (69.44%), Query Frame = 0

Query: 36  GIQKLDSICSKQGAKGINQDAAFFYQNYGAENTALL-GVFDGHGKNGHVVSNMVKNRLPL 95
           G  +  ++ ++QG KGINQDA   +  +G E+  +L GVFDGHG +GHVV+  V++ LPL
Sbjct: 49  GRSRTATVYTQQGRKGINQDAMLVWDGFGGEDDGVLCGVFDGHGPHGHVVARRVRDSLPL 108

Query: 96  LLIGQINERNRE-------EQFVTAFKVMDKEIKLHENLDCWCSGSTAVIAVKEGEDLVI 155
            L+    +   +       + F  A+K MDK+++ H +LDC+CSGSTAV  +K G DL +
Sbjct: 109 RLMSAARDSGADMPAAAWRKAFARAYKAMDKDLRSHPSLDCFCSGSTAVTVLKLGSDLYM 168

Query: 156 GNLGDSRAVLG--RMGEGGIEAVQLTTDLKPGLPDEGERIRRNNGRVLALKEEPHIQRVW 215
            N+GDSRAVLG      GG+ AVQLT DLKP +P E ERI++  GRV AL++EP + RVW
Sbjct: 169 ANIGDSRAVLGSREATGGGMVAVQLTVDLKPDVPSEAERIKKCRGRVFALQDEPEVPRVW 228

Query: 216 LPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRRLTSDDRFIVLATDGIWDVLSNDQVA 275
           LP +DAPGLAM+RAFGDF LK++G+I++P+  H  LT  D+F++LA+DG+WDVLSN +  
Sbjct: 229 LPFDDAPGLAMARAFGDFCLKDYGVISVPEFFHWSLTEKDQFVILASDGVWDVLSNQEAV 288

Query: 276 SIVWEAENEEAAAKAVVEAAAEAWK-RYPSSKLDDCTVVCLFLQNQAE 313
            IV  + +   AAK++VEAA   WK +YP+SK+DDC VVCL+L  + +
Sbjct: 289 DIVSASPSRSKAAKSLVEAATREWKTKYPTSKIDDCAVVCLYLDGKMD 336

BLAST of MS008658 vs. ExPASy Swiss-Prot
Match: O82637 (Probable protein phosphatase 2C 61 OS=Arabidopsis thaliana OX=3702 GN=At4g32950 PE=3 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.5e-76
Identity = 158/323 (48.92%), Postives = 214/323 (66.25%), Query Frame = 0

Query: 1   MGICI------STASSQIRHETEGSVICRNELIYEQNGSICGIQKLDSICSKQGAKGINQ 60
           MG C       ST  SQI   T+      N ++Y  +  +   Q L S+ S  G KG+NQ
Sbjct: 1   MGFCFCLSSGGSTDKSQIYEITDYGQ--ENAVLYSDHHVV--PQNLGSVSSLAGGKGLNQ 60

Query: 61  DAAFFYQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERN--REEQFV-- 120
           DAA  +  YG E  AL GVFDGHG  G  VS  V+N+LP +L+G +N  +  R+ + +  
Sbjct: 61  DAAILHLGYGTEEGALCGVFDGHGPRGAFVSKNVRNQLPSILLGHMNNHSVTRDWKLICE 120

Query: 121 TAFKVMDKEI-KLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAV-LGRMGEGGIEAV 180
           T+   MDK I K+ +  DC  SG+TAV+AVK G  +++ NLGDSRAV +G   +G  +  
Sbjct: 121 TSCLEMDKRILKVKKIHDCSASGTTAVLAVKHGNQVMVANLGDSRAVMIGTSEDGETKVA 180

Query: 181 QLTTDLKPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNH 240
           QLT DLKP +P E ERIR+ NGRVLAL+ EPHI RVWLP E+ PGLAMSRAFGDF LK++
Sbjct: 181 QLTNDLKPSVPSEAERIRKRNGRVLALESEPHILRVWLPTENRPGLAMSRAFGDFLLKSY 240

Query: 241 GIIALPDVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEA 300
           G+IA P VS  ++TS D+F++LA+DG+WDVLSN++VA++V ++ +E  AA  V EAA  A
Sbjct: 241 GVIATPQVSTHQITSSDQFLLLASDGVWDVLSNEEVATVVMKSASEAGAANEVAEAATNA 300

Query: 301 W-KRYPSSKLDDCTVVCLFLQNQ 311
           W +++P+ K+DD +VVCL L  +
Sbjct: 301 WIQKFPTVKIDDISVVCLSLNKK 319

BLAST of MS008658 vs. ExPASy Swiss-Prot
Match: Q9LR65 (Probable protein phosphatase 2C 1 OS=Arabidopsis thaliana OX=3702 GN=PPC6-6 PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.4e-76
Identity = 143/312 (45.83%), Postives = 207/312 (66.35%), Query Frame = 0

Query: 36  GIQKLDSICSKQGAKGINQDAAFFYQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLL 95
           G  +   I ++QG KGINQDA   ++++ +++    GVFDGHG +GH+V+  V++ LP+ 
Sbjct: 56  GKSRSSCIFTQQGRKGINQDAMIVWEDFMSKDVTFCGVFDGHGPHGHLVARKVRDSLPVK 115

Query: 96  LIGQIN----------------------------ERNR-----EEQFVTAFKVMDKEIKL 155
           L+  +N                            E ++     EE F+ +F  MDKE++ 
Sbjct: 116 LLSLLNSIKSKQNGPIGTRASKSDSLEAEKEESTEEDKLNFLWEEAFLKSFNAMDKELRS 175

Query: 156 HENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLG-RMGEGGIEAVQLTTDLKPGLPDE 215
           H NL+C+CSG TAV  +K+G +L +GN+GDSRA+LG +     + AVQLT DLKP LP E
Sbjct: 176 HPNLECFCSGCTAVTIIKQGSNLYMGNIGDSRAILGSKDSNDSMIAVQLTVDLKPDLPRE 235

Query: 216 GERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRRL 275
            ERI++  GRV AL++EP + RVWLP ++APGLAM+RAFGDF LK++G+I++P+ SHR L
Sbjct: 236 AERIKQCKGRVFALQDEPEVSRVWLPFDNAPGLAMARAFGDFCLKDYGVISIPEFSHRVL 295

Query: 276 TSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWK-RYPSSKLDDC 313
           T  D+FIVLA+DG+WDVLSN++V  +V  A +  +AA+ VV++A   WK +YP+SK+DDC
Sbjct: 296 TDRDQFIVLASDGVWDVLSNEEVVEVVASATSRASAARLVVDSAVREWKLKYPTSKMDDC 355

BLAST of MS008658 vs. ExPASy Swiss-Prot
Match: Q6K6N7 (Probable protein phosphatase 2C 14 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0471500 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 4.5e-76
Identity = 147/307 (47.88%), Postives = 206/307 (67.10%), Query Frame = 0

Query: 42  SICSKQGAKGINQDAAFFYQNY-GAENTALLGVFDGHGKNGHVVSNMVKNRLPLLL---- 101
           S+ + QG KG+NQDA   ++N+   E+T   GVFDGHG NGH+V+  V++ LP+ L    
Sbjct: 131 SLYTLQGKKGVNQDAMIVWENFCSREDTIFCGVFDGHGPNGHLVAKRVRDLLPIKLGADL 190

Query: 102 -------------------------IGQINERNRE---------EQFVTAFKVMDKEIKL 161
                                    +G+  E+N E           F+ AF VMD+++KL
Sbjct: 191 GTDEGRQTSTSSIKSNGDETGSPGNMGRDAEQNGEYPEIFTALRTSFLRAFNVMDRDLKL 250

Query: 162 HENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLG-RMGEGGIEAVQLTTDLKPGLPDE 221
           H+++DC+ SG+TAV  +K+G +L+IGNLGDSRA+LG R  +  + AVQLT DLKP +P E
Sbjct: 251 HKSIDCFFSGTTAVAVLKQGRNLIIGNLGDSRAILGTRDKDNQLMAVQLTVDLKPNIPSE 310

Query: 222 GERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRRL 281
            +RIR+  GR+ AL EEP + RVWLP  ++PGLAM+RAFGDF LK++G+I++P+VS+ R+
Sbjct: 311 AQRIRQRRGRIFALPEEPEVARVWLPKYNSPGLAMARAFGDFCLKDYGLISMPEVSYHRI 370

Query: 282 TSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWK-RYPSSKLDDC 308
           T  D F+VLATDG+WDVLSN +V SIV  A +  +AA+ +VE+A  AW+ R+P+SK+DDC
Sbjct: 371 TEKDEFVVLATDGVWDVLSNTEVVSIVNRATSRASAARLLVESAHRAWRARFPTSKIDDC 430

BLAST of MS008658 vs. ExPASy TrEMBL
Match: A0A6J1DKZ8 (Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111022032 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.2e-174
Identity = 308/312 (98.72%), Postives = 308/312 (98.72%), Query Frame = 0

Query: 1   MGICISTASSQIRHETEGSVICRNELIYEQNGSICGIQKLDSICSKQGAKGINQDAAFFY 60
           MGICISTASSQIRHETEGSVICRNELIYEQNGSICGIQKLD ICSKQGAKGINQDAAFFY
Sbjct: 1   MGICISTASSQIRHETEGSVICRNELIYEQNGSICGIQKLDYICSKQGAKGINQDAAFFY 60

Query: 61  QNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNREEQFVTAFKVMDKEI 120
           QNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNREEQFVTAFKVMDKEI
Sbjct: 61  QNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNREEQFVTAFKVMDKEI 120

Query: 121 KLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEAVQLTTDLKPGLPD 180
           KLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEAVQLTTDLKPGLPD
Sbjct: 121 KLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEAVQLTTDLKPGLPD 180

Query: 181 EGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRR 240
           EGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRR
Sbjct: 181 EGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRR 240

Query: 241 LTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWKRYPSSKLDDC 300
           LTSDDRFIVLATDGIWDVLSNDQV SIVWEAENEEAAAKA VEAAAEAWKRYPSSKLDDC
Sbjct: 241 LTSDDRFIVLATDGIWDVLSNDQVVSIVWEAENEEAAAKAAVEAAAEAWKRYPSSKLDDC 300

Query: 301 TVVCLFLQNQAE 313
           TVV LFLQNQAE
Sbjct: 301 TVVWLFLQNQAE 312

BLAST of MS008658 vs. ExPASy TrEMBL
Match: A0A6J1J2J0 (Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111481232 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.5e-124
Identity = 235/318 (73.90%), Postives = 261/318 (82.08%), Query Frame = 0

Query: 1   MGICISTASSQIRHETEGSVIC-RNELIYEQNGSICGIQKLDSICSKQGAKGINQDAAFF 60
           MGICIS ASSQIRHET+ + IC  NEL +E+       Q+ DS+CSKQG KGINQDAAF 
Sbjct: 1   MGICISAASSQIRHETKVTAICTTNELGFEE-------QRPDSVCSKQGNKGINQDAAFV 60

Query: 61  YQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNR-----EEQFVTAFK 120
           YQ YG +  AL GVFDGHG NGH VSNM KNRLP LL+  +++        +E  VTAFK
Sbjct: 61  YQEYGEQGRALFGVFDGHGTNGHTVSNMAKNRLPPLLLSAMSKAETNFVGWKEAMVTAFK 120

Query: 121 VMDKEIKLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEAVQLTTDL 180
           VMDKEIKL E LDC  SG+TAVI +K+GEDLVI NLGDSRAV+GR+ +  IEAVQLTTDL
Sbjct: 121 VMDKEIKLQETLDCSFSGTTAVIILKQGEDLVIANLGDSRAVMGRVTDDDIEAVQLTTDL 180

Query: 181 KPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALP 240
           KPGLPDE ERIR  NGRVLAL+EEPHIQRVWLPNEDAPGLAMSRAFGDFALK HGIIALP
Sbjct: 181 KPGLPDEAERIRNCNGRVLALQEEPHIQRVWLPNEDAPGLAMSRAFGDFALKEHGIIALP 240

Query: 241 DVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWKRYPS 300
           DVSHRRL  DD FIVLATDGIWDVLSN++VASIV +AE+EEAAAKAVVEAA EAWKRYP+
Sbjct: 241 DVSHRRLAPDDHFIVLATDGIWDVLSNNEVASIVSKAESEEAAAKAVVEAATEAWKRYPA 300

Query: 301 SKLDDCTVVCLFLQNQAE 313
           SKLDDCTVVC FLQN+ +
Sbjct: 301 SKLDDCTVVCHFLQNKRQ 311

BLAST of MS008658 vs. ExPASy TrEMBL
Match: A0A6J1FR27 (Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111446470 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.5e-124
Identity = 235/318 (73.90%), Postives = 262/318 (82.39%), Query Frame = 0

Query: 1   MGICISTASSQIRHETEGSVIC-RNELIYEQNGSICGIQKLDSICSKQGAKGINQDAAFF 60
           MGICIS ASSQIRHET+ + IC  NEL +E+       Q+ DS+CSKQG KGINQDAAF 
Sbjct: 1   MGICISAASSQIRHETKVTAICAANELAFEE-------QRSDSVCSKQGNKGINQDAAFV 60

Query: 61  YQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNRE-----EQFVTAFK 120
           YQ YG +  AL GVFDGHG NGH VSNM K+RLP LL+  +++   +     E  VTAFK
Sbjct: 61  YQEYGEQGRALFGVFDGHGTNGHTVSNMAKSRLPPLLLSAMSKAETDFVGWKEAMVTAFK 120

Query: 121 VMDKEIKLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGEGGIEAVQLTTDL 180
           VMDKEIKL ENLDC  SG+TAVI +K+GEDLVI NLGDSRAV+GR+ E GIEAVQLTTDL
Sbjct: 121 VMDKEIKLQENLDCSFSGTTAVIILKQGEDLVIANLGDSRAVMGRVTENGIEAVQLTTDL 180

Query: 181 KPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALP 240
           KPGLPDE ERIR+ NGRVLAL+EEPHIQRVWLP EDAPGLAMSRAFGDFALK HGIIALP
Sbjct: 181 KPGLPDEAERIRKCNGRVLALQEEPHIQRVWLPKEDAPGLAMSRAFGDFALKEHGIIALP 240

Query: 241 DVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWKRYPS 300
           DVS+RRL  DD FIVLATDGIWDVLSND+VASIV +AE+EEAAAKAVVEAA EAWKRYP+
Sbjct: 241 DVSYRRLAPDDHFIVLATDGIWDVLSNDEVASIVSKAESEEAAAKAVVEAATEAWKRYPA 300

Query: 301 SKLDDCTVVCLFLQNQAE 313
           SKLDD TVVC FLQN+ +
Sbjct: 301 SKLDDRTVVCHFLQNKRQ 311

BLAST of MS008658 vs. ExPASy TrEMBL
Match: A0A0A0L2Q1 (Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=Csa_4G645920 PE=3 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 9.6e-122
Identity = 236/322 (73.29%), Postives = 266/322 (82.61%), Query Frame = 0

Query: 1   MGICISTASSQIRHETE--GSVICRNELIYEQNGSICGIQKLDSICSKQGAKGINQDAAF 60
           MGICIS+ASSQIRHET   GS+I   +  Y+   S+CGIQKL+SICSKQG+KGINQDAAF
Sbjct: 1   MGICISSASSQIRHETNKVGSIIIEED-HYKSCYSVCGIQKLESICSKQGSKGINQDAAF 60

Query: 61  FYQNYG-AENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNR----------EE 120
            YQ YG  E + L GVFDGHG NGH+VSN+VKNRLP LL+G++  ++           +E
Sbjct: 61  AYQEYGEVEESDLFGVFDGHGPNGHIVSNLVKNRLPSLLLGEVTAKSSLVKRKSFNAWKE 120

Query: 121 QFVTAFKVMDKEIKLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGE-GGIE 180
            F T+FKVMDKEIKL ENLD  CSGSTAV+ VK+ + LVIGNLGDSRAV+GRM E GGI+
Sbjct: 121 AFETSFKVMDKEIKLQENLDGSCSGSTAVVMVKQEDYLVIGNLGDSRAVMGRMREDGGIK 180

Query: 181 AVQLTTDLKPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALK 240
           AVQLTTDLKPGL  EGERIRR  GRVLALK+E HIQRVWLPNED+PGLAMSRAFGDFALK
Sbjct: 181 AVQLTTDLKPGLESEGERIRRCKGRVLALKDEAHIQRVWLPNEDSPGLAMSRAFGDFALK 240

Query: 241 NHGIIALPDVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAA 300
           ++GII LPDVS R LTS DRFIVLATDGIWDVLSND+VASIVW AE+EEAAAKAVV+AA 
Sbjct: 241 DYGIINLPDVSFRPLTSLDRFIVLATDGIWDVLSNDEVASIVWAAESEEAAAKAVVDAAT 300

Query: 301 EAWKRYPSSKLDDCTVVCLFLQ 309
           EAWKR+PSSK DDCTVVC FLQ
Sbjct: 301 EAWKRWPSSKQDDCTVVCHFLQ 321

BLAST of MS008658 vs. ExPASy TrEMBL
Match: A0A1S3BTW6 (Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103493643 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.8e-121
Identity = 235/322 (72.98%), Postives = 268/322 (83.23%), Query Frame = 0

Query: 1   MGICISTASSQIRHETE--GSVICRNELIYEQNGSICGIQKLDSICSKQGAKGINQDAAF 60
           MGICIS+ASSQIRHET   GS+I  ++  Y+   S+CGIQKL+SICSKQG+KGINQDAAF
Sbjct: 1   MGICISSASSQIRHETNKVGSIIEEDD-HYKSCYSVCGIQKLESICSKQGSKGINQDAAF 60

Query: 61  FYQNYG-AENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNR----------EE 120
            YQ +G  E + L GVFDGHG+NGH+VSN+VKNRLP LL+ ++  ++           +E
Sbjct: 61  AYQEFGEVEESDLFGVFDGHGQNGHIVSNLVKNRLPSLLLREVTAKSSLVKRKSFNTWKE 120

Query: 121 QFVTAFKVMDKEIKLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRM-GEGGIE 180
            F T+FKVMDKEIKL ENLD   SGSTAV+ VK+G+ LVIGNLGDSRAV+GRM  +GGI+
Sbjct: 121 AFETSFKVMDKEIKLQENLDGSFSGSTAVVIVKQGDYLVIGNLGDSRAVMGRMRDDGGIK 180

Query: 181 AVQLTTDLKPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALK 240
           AVQLTTDLKPGL  EGERIRR  GRVLALK+E HIQRVWLPNEDAPGLAMSRAFGDFALK
Sbjct: 181 AVQLTTDLKPGLASEGERIRRCKGRVLALKDEAHIQRVWLPNEDAPGLAMSRAFGDFALK 240

Query: 241 NHGIIALPDVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAA 300
           ++GII LPDVS R LTS DRFIVLATDGIWDVLSND+VASIVW AE+EEAAAKAVV+AAA
Sbjct: 241 DYGIINLPDVSFRPLTSHDRFIVLATDGIWDVLSNDEVASIVWAAESEEAAAKAVVDAAA 300

Query: 301 EAWKRYPSSKLDDCTVVCLFLQ 309
           EAWKRYPSSK DDCTV+C FLQ
Sbjct: 301 EAWKRYPSSKHDDCTVICHFLQ 321

BLAST of MS008658 vs. TAIR 10
Match: AT5G26010.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 326.6 bits (836), Expect = 2.1e-89
Identity = 177/333 (53.15%), Postives = 230/333 (69.07%), Query Frame = 0

Query: 1   MGICISTASSQIR-HET----EGSVICRNELI-YEQNGSICGIQKLDSICSKQGAKGINQ 60
           MG C S  SSQ   HE     +G+V+C  E    +Q+     + +L S+CS QG K +NQ
Sbjct: 1   MGHCFSLPSSQSEIHEDNEHGDGNVVCYGEEFGLDQD---LPVHRLGSVCSIQGTKVLNQ 60

Query: 61  DAAFFYQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERNREEQF----- 120
           D A  YQ YG  +T L GVFDGHGKNGH+VS MV+NRLP +L+    E N+E        
Sbjct: 61  DHAVLYQGYGTRDTELCGVFDGHGKNGHMVSKMVRNRLPSVLLALKEELNQESNVCEEEA 120

Query: 121 -------VTAFKVMDKEIKLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLGRMGE 180
                   TAF+++D+E+ L +  +C  SGST V+A+ +G+DLVI NLGDSRAVLG M E
Sbjct: 121 SKWEKACFTAFRLIDRELNL-QVFNCSFSGSTGVVAITQGDDLVIANLGDSRAVLGTMTE 180

Query: 181 GG-IEAVQLTTDLKPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFG 240
            G I+AVQLT+DL P +P E ERIR   GRV A+K EP  QRVWLPN++ PGLAMSRAFG
Sbjct: 181 DGEIKAVQLTSDLTPDVPSEAERIRMCKGRVFAMKTEPSSQRVWLPNQNIPGLAMSRAFG 240

Query: 241 DFALKNHGIIALPDVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEA-AAKA 300
           DF LK+HG+IA+P++S  R+TS D+F+VLATDG+WD+LSND+V S++W +  ++A AAK 
Sbjct: 241 DFRLKDHGVIAVPEISQHRITSKDQFLVLATDGVWDMLSNDEVVSLIWSSGKKQASAAKM 300

Query: 301 VVEAAAEAW-KRYPSSKLDDCTVVCLFLQNQAE 313
           V EAA  AW KR   +K+DD TV+CLFLQN+ +
Sbjct: 301 VAEAAEAAWKKRLKYTKVDDITVICLFLQNKEQ 329

BLAST of MS008658 vs. TAIR 10
Match: AT4G32950.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.1e-77
Identity = 158/323 (48.92%), Postives = 214/323 (66.25%), Query Frame = 0

Query: 1   MGICI------STASSQIRHETEGSVICRNELIYEQNGSICGIQKLDSICSKQGAKGINQ 60
           MG C       ST  SQI   T+      N ++Y  +  +   Q L S+ S  G KG+NQ
Sbjct: 1   MGFCFCLSSGGSTDKSQIYEITDYGQ--ENAVLYSDHHVV--PQNLGSVSSLAGGKGLNQ 60

Query: 61  DAAFFYQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLLIGQINERN--REEQFV-- 120
           DAA  +  YG E  AL GVFDGHG  G  VS  V+N+LP +L+G +N  +  R+ + +  
Sbjct: 61  DAAILHLGYGTEEGALCGVFDGHGPRGAFVSKNVRNQLPSILLGHMNNHSVTRDWKLICE 120

Query: 121 TAFKVMDKEI-KLHENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAV-LGRMGEGGIEAV 180
           T+   MDK I K+ +  DC  SG+TAV+AVK G  +++ NLGDSRAV +G   +G  +  
Sbjct: 121 TSCLEMDKRILKVKKIHDCSASGTTAVLAVKHGNQVMVANLGDSRAVMIGTSEDGETKVA 180

Query: 181 QLTTDLKPGLPDEGERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNH 240
           QLT DLKP +P E ERIR+ NGRVLAL+ EPHI RVWLP E+ PGLAMSRAFGDF LK++
Sbjct: 181 QLTNDLKPSVPSEAERIRKRNGRVLALESEPHILRVWLPTENRPGLAMSRAFGDFLLKSY 240

Query: 241 GIIALPDVSHRRLTSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEA 300
           G+IA P VS  ++TS D+F++LA+DG+WDVLSN++VA++V ++ +E  AA  V EAA  A
Sbjct: 241 GVIATPQVSTHQITSSDQFLLLASDGVWDVLSNEEVATVVMKSASEAGAANEVAEAATNA 300

Query: 301 W-KRYPSSKLDDCTVVCLFLQNQ 311
           W +++P+ K+DD +VVCL L  +
Sbjct: 301 WIQKFPTVKIDDISVVCLSLNKK 319

BLAST of MS008658 vs. TAIR 10
Match: AT1G03590.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.4e-77
Identity = 143/312 (45.83%), Postives = 207/312 (66.35%), Query Frame = 0

Query: 36  GIQKLDSICSKQGAKGINQDAAFFYQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLL 95
           G  +   I ++QG KGINQDA   ++++ +++    GVFDGHG +GH+V+  V++ LP+ 
Sbjct: 56  GKSRSSCIFTQQGRKGINQDAMIVWEDFMSKDVTFCGVFDGHGPHGHLVARKVRDSLPVK 115

Query: 96  LIGQIN----------------------------ERNR-----EEQFVTAFKVMDKEIKL 155
           L+  +N                            E ++     EE F+ +F  MDKE++ 
Sbjct: 116 LLSLLNSIKSKQNGPIGTRASKSDSLEAEKEESTEEDKLNFLWEEAFLKSFNAMDKELRS 175

Query: 156 HENLDCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLG-RMGEGGIEAVQLTTDLKPGLPDE 215
           H NL+C+CSG TAV  +K+G +L +GN+GDSRA+LG +     + AVQLT DLKP LP E
Sbjct: 176 HPNLECFCSGCTAVTIIKQGSNLYMGNIGDSRAILGSKDSNDSMIAVQLTVDLKPDLPRE 235

Query: 216 GERIRRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRRL 275
            ERI++  GRV AL++EP + RVWLP ++APGLAM+RAFGDF LK++G+I++P+ SHR L
Sbjct: 236 AERIKQCKGRVFALQDEPEVSRVWLPFDNAPGLAMARAFGDFCLKDYGVISIPEFSHRVL 295

Query: 276 TSDDRFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWK-RYPSSKLDDC 313
           T  D+FIVLA+DG+WDVLSN++V  +V  A +  +AA+ VV++A   WK +YP+SK+DDC
Sbjct: 296 TDRDQFIVLASDGVWDVLSNEEVVEVVASATSRASAARLVVDSAVREWKLKYPTSKMDDC 355

BLAST of MS008658 vs. TAIR 10
Match: AT4G03415.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 282.3 bits (721), Expect = 4.6e-76
Identity = 144/308 (46.75%), Postives = 200/308 (64.94%), Query Frame = 0

Query: 43  ICSKQGAKGINQDAAFFYQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLL------ 102
           I ++QG KGINQDA   ++++ +E+    GVFDGHG  GH+V+  V++ LP+ L      
Sbjct: 70  IFTQQGRKGINQDAMIVWEDFMSEDVTFCGVFDGHGPYGHLVARKVRDTLPVKLQFFFQT 129

Query: 103 ------------------IGQINERNRE------------EQFVTAFKVMDKEIKLHENL 162
                                + E  +E            E F+ +FK MDKE++ H NL
Sbjct: 130 LQSKQNCSKGTRFRRNSSKSAVQEAVKEGSDEDKLKGLWGEAFLKSFKAMDKELRSHPNL 189

Query: 163 DCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLG-RMGEGGIEAVQLTTDLKPGLPDEGERI 222
           DC+CSGST V  +K+G +L +GN+GDSRA+LG +     + A QLT DLKP LP E ERI
Sbjct: 190 DCFCSGSTGVTILKQGSNLFMGNIGDSRAILGSKDSNDSMVATQLTVDLKPDLPREAERI 249

Query: 223 RRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRRLTSDD 282
           +R  GRV A+++EP + RVWLP +DAPGLAM+RAFGDF LK +G+I++P+ +HR LT  D
Sbjct: 250 KRCKGRVFAMEDEPEVPRVWLPYDDAPGLAMARAFGDFCLKEYGVISVPEFTHRVLTDRD 309

Query: 283 RFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWK-RYPSSKLDDCTVVC 313
           +FIVLA+DG+WDVLSN++V  IV  A +  +AA+ +V +AA  WK +YP+SK+DDC VVC
Sbjct: 310 QFIVLASDGVWDVLSNEEVVDIVASATSRASAARTLVNSAAREWKLKYPTSKMDDCAVVC 369

BLAST of MS008658 vs. TAIR 10
Match: AT4G03415.2 (Protein phosphatase 2C family protein )

HSP 1 Score: 282.3 bits (721), Expect = 4.6e-76
Identity = 144/308 (46.75%), Postives = 200/308 (64.94%), Query Frame = 0

Query: 43  ICSKQGAKGINQDAAFFYQNYGAENTALLGVFDGHGKNGHVVSNMVKNRLPLLL------ 102
           I ++QG KGINQDA   ++++ +E+    GVFDGHG  GH+V+  V++ LP+ L      
Sbjct: 70  IFTQQGRKGINQDAMIVWEDFMSEDVTFCGVFDGHGPYGHLVARKVRDTLPVKLQFFFQT 129

Query: 103 ------------------IGQINERNRE------------EQFVTAFKVMDKEIKLHENL 162
                                + E  +E            E F+ +FK MDKE++ H NL
Sbjct: 130 LQSKQNCSKGTRFRRNSSKSAVQEAVKEGSDEDKLKGLWGEAFLKSFKAMDKELRSHPNL 189

Query: 163 DCWCSGSTAVIAVKEGEDLVIGNLGDSRAVLG-RMGEGGIEAVQLTTDLKPGLPDEGERI 222
           DC+CSGST V  +K+G +L +GN+GDSRA+LG +     + A QLT DLKP LP E ERI
Sbjct: 190 DCFCSGSTGVTILKQGSNLFMGNIGDSRAILGSKDSNDSMVATQLTVDLKPDLPREAERI 249

Query: 223 RRNNGRVLALKEEPHIQRVWLPNEDAPGLAMSRAFGDFALKNHGIIALPDVSHRRLTSDD 282
           +R  GRV A+++EP + RVWLP +DAPGLAM+RAFGDF LK +G+I++P+ +HR LT  D
Sbjct: 250 KRCKGRVFAMEDEPEVPRVWLPYDDAPGLAMARAFGDFCLKEYGVISVPEFTHRVLTDRD 309

Query: 283 RFIVLATDGIWDVLSNDQVASIVWEAENEEAAAKAVVEAAAEAWK-RYPSSKLDDCTVVC 313
           +FIVLA+DG+WDVLSN++V  IV  A +  +AA+ +V +AA  WK +YP+SK+DDC VVC
Sbjct: 310 QFIVLASDGVWDVLSNEEVVDIVASATSRASAARTLVNSAAREWKLKYPTSKMDDCAVVC 369

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154915.14.5e-17498.72probable protein phosphatase 2C 72 [Momordica charantia][more]
XP_038899305.19.2e-12775.08probable protein phosphatase 2C 72 [Benincasa hispida][more]
XP_023524315.12.9e-12574.21probable protein phosphatase 2C 72 [Cucurbita pepo subsp. pepo][more]
KAG6608387.16.6e-12574.21putative protein phosphatase 2C 72, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022941073.17.3e-12473.90probable protein phosphatase 2C 72 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9XGZ93.0e-8853.15Probable protein phosphatase 2C 72 OS=Arabidopsis thaliana OX=3702 GN=At5g26010 ... [more]
Q8H4S68.2e-7849.65Probable protein phosphatase 2C 64 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
O826371.5e-7648.92Probable protein phosphatase 2C 61 OS=Arabidopsis thaliana OX=3702 GN=At4g32950 ... [more]
Q9LR653.4e-7645.83Probable protein phosphatase 2C 1 OS=Arabidopsis thaliana OX=3702 GN=PPC6-6 PE=1... [more]
Q6K6N74.5e-7647.88Probable protein phosphatase 2C 14 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Match NameE-valueIdentityDescription
A0A6J1DKZ82.2e-17498.72Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1J2J03.5e-12473.90Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111481232... [more]
A0A6J1FR273.5e-12473.90Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC1114464... [more]
A0A0A0L2Q19.6e-12273.29Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=Csa_4G645920 ... [more]
A0A1S3BTW62.8e-12172.98Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103493643 PE=... [more]
Match NameE-valueIdentityDescription
AT5G26010.12.1e-8953.15Protein phosphatase 2C family protein [more]
AT4G32950.11.1e-7748.92Protein phosphatase 2C family protein [more]
AT1G03590.12.4e-7745.83Protein phosphatase 2C family protein [more]
AT4G03415.14.6e-7646.75Protein phosphatase 2C family protein [more]
AT4G03415.24.6e-7646.75Protein phosphatase 2C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001932PPM-type phosphatase domainSMARTSM00332PP2C_4coord: 24..305
e-value: 1.3E-69
score: 247.3
IPR001932PPM-type phosphatase domainPFAMPF00481PP2Ccoord: 44..285
e-value: 1.7E-45
score: 155.7
IPR001932PPM-type phosphatase domainPROSITEPS51746PPM_2coord: 40..307
score: 46.368454
IPR001932PPM-type phosphatase domainCDDcd00143PP2Cccoord: 42..307
e-value: 6.81587E-69
score: 213.342
IPR036457PPM-type phosphatase domain superfamilyGENE3D3.60.40.10coord: 39..312
e-value: 1.3E-74
score: 253.2
IPR036457PPM-type phosphatase domain superfamilySUPERFAMILY81606PP2C-likecoord: 42..309
NoneNo IPR availablePANTHERPTHR47992:SF57PROTEIN PHOSPHATASE 2C 72-RELATEDcoord: 1..310
NoneNo IPR availablePANTHERPTHR47992ALPHABET, ISOFORM E-RELATEDcoord: 1..310
IPR000222PPM-type phosphatase, divalent cation bindingPROSITEPS01032PPM_1coord: 70..78

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS008658.1MS008658.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0043169 cation binding
molecular_function GO:0016791 phosphatase activity