MS008616 (gene) Bitter gourd (TR) v1

BLAST of MS008616 vs. NCBI nr
Match: XP_022145048.1 (protein BIG GRAIN 1-like B [Momordica charantia])

HSP 1 Score: 801.6 bits (2069), Expect = 3.3e-228
Identity = 425/429 (99.07%), Postives = 426/429 (99.30%), Query Frame = 0

Query: 1   MNGQEIRARGERFRNGGDGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMI 60
           MNGQEIRARGERFRNGGDGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMI
Sbjct: 1   MNGQEIRARGERFRNGGDGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMI 60

Query: 61  GKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVL 120
           GKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVL
Sbjct: 61  GKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVL 120

Query: 121 FFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQR 180
           FFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQR
Sbjct: 121 FFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQR 180

Query: 181 QNREKPQSKQSNQFVINKESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFT 240
           QNREKPQSKQSNQFVINKESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFT
Sbjct: 181 QNREKPQSKQSNQFVINKESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFT 240

Query: 241 AGTPKKTGNSMTSTTSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDR 300
           AGTPKKTGNSMTSTTSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDR
Sbjct: 241 AGTPKKTGNSMTSTTSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDR 300

Query: 301 RTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDYQQNRKKN 360
           RTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDYQQNRKKN
Sbjct: 301 RTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDYQQNRKKN 360

Query: 361 DFLIRNFHHPDNDLYEEEEEE---DRASCSSSDLFELDHLAVMGGDRYHEELPVYETTTR 420
           DFLIRNFHHPDNDLYEEEEEE   DRASCSSSDLFELDHLAVMGGDRYHEELPVYETTTR
Sbjct: 361 DFLIRNFHHPDNDLYEEEEEEEEDDRASCSSSDLFELDHLAVMGGDRYHEELPVYETTTR 420

Query: 421 VETNRAIRH 427
           V+TNRAIRH
Sbjct: 421 VDTNRAIRH 429

BLAST of MS008616 vs. NCBI nr
Match: XP_038899103.1 (protein BIG GRAIN 1-like A [Benincasa hispida])

HSP 1 Score: 684.9 bits (1766), Expect = 4.5e-193
Identity = 377/435 (86.67%), Postives = 399/435 (91.72%), Query Frame = 0

Query: 1   MNGQEIRARGERFRNGGDG-QSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKM 60
           M+G+E+R RGERFRNGG   QSFSSSLLDEIYRSIDDGGDKRSGELKFYR KV+KKQGK 
Sbjct: 1   MSGEEVRVRGERFRNGGGNVQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKT 60

Query: 61  IGKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDV 120
           IGKA SD+EDEEIASLHRALLIERWIEKKVAEKVS QRRRSLTEAE KFQLYQHDREEDV
Sbjct: 61  IGKATSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEIKFQLYQHDREEDV 120

Query: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQ 180
           LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTS+SAP PEK+E KQ
Sbjct: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKTEAKQ 180

Query: 181 RQNREKPQSKQSNQFVINKE-SRDFDE----KSKSRALKIYSNLKKVKQPISPGGRLSSF 240
           RQ+REKPQSK SNQFV NKE S  FDE    KSKSRALKIYSNLKKVKQPISPGGRLSSF
Sbjct: 181 RQSREKPQSKHSNQFVENKEKSGGFDENAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240

Query: 241 INSIFTAGTPKKTGNSMTSTT-SEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSRE 300
           +NSIFTAGTPKKTGNS++ST+ SEDPN+ERKSKSGQTSTCSSA SFSRSCL+KNSPCS E
Sbjct: 241 LNSIFTAGTPKKTGNSVSSTSVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSE 300

Query: 301 KLRNSDRRTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEA-TAAARKILTD 360
           KLRN DRRTVRFYPVSTIVDEYCRPCGHKSLYEE+ KFQ+K K +T E+ TAAARKILTD
Sbjct: 301 KLRNGDRRTVRFYPVSTIVDEYCRPCGHKSLYEEDPKFQIKNKAETAESTTAAARKILTD 360

Query: 361 YQQNRKKNDFLIRNFHHPDNDLYEEEEEEDRASCSSSDLFELDHLAVM-GGDRYHEELPV 420
           YQQ +KKNDFLIRNFHHPDNDL EE+E+E  ASCSSSDLFELDHL VM GGDRY EELPV
Sbjct: 361 YQQVKKKNDFLIRNFHHPDNDLSEEDEDE-TASCSSSDLFELDHLTVMNGGDRYREELPV 420

Query: 421 YETTTRVETNRAIRH 427
           YETTTRV+TNRAIRH
Sbjct: 421 YETTTRVDTNRAIRH 434

BLAST of MS008616 vs. NCBI nr
Match: XP_022977029.1 (protein BIG GRAIN 1-like B [Cucurbita maxima])

HSP 1 Score: 679.5 bits (1752), Expect = 1.9e-191
Identity = 375/436 (86.01%), Postives = 393/436 (90.14%), Query Frame = 0

Query: 1   MNGQEIRARGERFRNGGD-GQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKM 60
           M+GQE+R RGERFR+GG   QSFSSSLLDEIYRSIDDGGDKRSGELKFYR KV+KKQGKM
Sbjct: 1   MSGQEVRVRGERFRDGGGIVQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKM 60

Query: 61  IGKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDV 120
           IGKAQSD+EDEEIASLHRALLIERWIEKKVAEKVS QRRRSLTEAE KFQLYQ DREEDV
Sbjct: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEIKFQLYQQDREEDV 120

Query: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQ 180
           LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTS+SAP PEK+E KQ
Sbjct: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKTEEKQ 180

Query: 181 RQNREKPQSKQSNQFVINKE-SRDFDE----KSKSRALKIYSNLKKVKQPISPGGRLSSF 240
           RQ+REKP SKQ NQFV N E SR FDE    KSKSRALKIYSNLKKVKQPISPGGRLSSF
Sbjct: 181 RQSREKPPSKQINQFVENNEKSRGFDESAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240

Query: 241 INSIFTAGTPKKTGNSMTST-TSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSRE 300
           +NSIFTAGTPKKTGNS+TST  SED N+ERKSKSGQTSTCSSA SFSRSCL+KNSPCS E
Sbjct: 241 LNSIFTAGTPKKTGNSVTSTRASEDHNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSE 300

Query: 301 KLRNSDRRTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDY 360
           KLRN+D RTVRFYPVSTIVDEY RPCGHKSL EEE KF+VK K +  E+TAA RKI+TDY
Sbjct: 301 KLRNADTRTVRFYPVSTIVDEYSRPCGHKSLCEEEPKFEVKNKAEAAESTAAVRKIVTDY 360

Query: 361 QQNRKKNDFLIRNFHHPDNDLYEEEEEE---DRASCSSSDLFELDHLAVMGGDRYHEELP 420
           QQ RKKNDFLIR FHHPDNDLYEEEEEE   D ASCSSSDLFELDHL VM GDRY EELP
Sbjct: 361 QQIRKKNDFLIRKFHHPDNDLYEEEEEEEEDDTASCSSSDLFELDHLTVMSGDRYREELP 420

Query: 421 VYETTTRVETNRAIRH 427
           VYETTTRV+TNRAIRH
Sbjct: 421 VYETTTRVDTNRAIRH 436

BLAST of MS008616 vs. NCBI nr
Match: XP_022936713.1 (protein BIG GRAIN 1-like B [Cucurbita moschata])

HSP 1 Score: 678.7 bits (1750), Expect = 3.2e-191
Identity = 373/434 (85.94%), Postives = 395/434 (91.01%), Query Frame = 0

Query: 1   MNGQEIRARGERFRNGGD-GQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKM 60
           M+GQE+RARGERFR+GG   QSFSSSLLDEIYRSIDDGGDKRSGELKFYR KV+KKQGKM
Sbjct: 1   MSGQEVRARGERFRDGGGIVQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKM 60

Query: 61  IGKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDV 120
           IGKAQSD+EDEEIASLHRALLIERWIEKKVAEKVS QRRRSLTEAE KFQLYQ +REEDV
Sbjct: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEIKFQLYQQEREEDV 120

Query: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQ 180
           LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTS+SAP PEK+E KQ
Sbjct: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKTEEKQ 180

Query: 181 RQNREKPQSKQSNQFVINKE-SRDFDE----KSKSRALKIYSNLKKVKQPISPGGRLSSF 240
           RQ+REKP +KQ NQFV N E SR FDE    KSKSRALKIYSNLKKVKQPISPGGRLSSF
Sbjct: 181 RQSREKPPAKQINQFVENNEKSRGFDESAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240

Query: 241 INSIFTAGTPKKTGNSMTST-TSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSRE 300
           +NSIFTAGTPKKTGNS+TST  SED N+ERKSKSGQTSTCSSA SFSRSCL+KNSPCS E
Sbjct: 241 LNSIFTAGTPKKTGNSVTSTGVSEDHNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSE 300

Query: 301 KLRNSDRRTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDY 360
           KLRN+D RTVRFYPVSTIVDEY RPCGHKSL EE+ KF+VK K +T EATAA RKI+TDY
Sbjct: 301 KLRNADTRTVRFYPVSTIVDEYSRPCGHKSLCEEQPKFEVKNKAETAEATAAVRKIVTDY 360

Query: 361 QQNRKKNDFLIRNFHHPDNDLYEEE-EEEDRASCSSSDLFELDHLAVMGGDRYHEELPVY 420
           QQ RKKNDFLIR FHHPDNDLYEEE EE+D ASCSSSDLFELDHL VM GDRY EELPVY
Sbjct: 361 QQIRKKNDFLIRKFHHPDNDLYEEEQEEDDTASCSSSDLFELDHLTVMSGDRYREELPVY 420

Query: 421 ETTTRVETNRAIRH 427
           ETTTR++TNRAIRH
Sbjct: 421 ETTTRMDTNRAIRH 434

BLAST of MS008616 vs. NCBI nr
Match: KAG7024366.1 (Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 675.6 bits (1742), Expect = 2.7e-190
Identity = 375/435 (86.21%), Postives = 395/435 (90.80%), Query Frame = 0

Query: 1   MNGQEIRARGERFRNGGD-GQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKM 60
           M+GQE+RARGERFR+GG   QSFSSSLLDEIYRSIDDGGDKRSGELKFYR KV+KKQGKM
Sbjct: 1   MSGQEVRARGERFRDGGGIVQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKM 60

Query: 61  IGKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDV 120
           IGKAQSD+EDEEIASLHRALLIERWIEKKVAEKVS  RRRSLTEAE KFQLYQ +REEDV
Sbjct: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSALRRRSLTEAEIKFQLYQQEREEDV 120

Query: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQ 180
           LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTS+SAP PEK+E KQ
Sbjct: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKTEEKQ 180

Query: 181 RQNREKPQSKQSNQFVINKE-SRDFDE----KSKSRALKIYSNLKKVKQPISPGGRLSSF 240
           RQ+REKP SKQ NQFV N E SR FDE    KSKSRALKIYSNLKKVKQPISPGGRLSSF
Sbjct: 181 RQSREKPPSKQINQFVENNEKSRGFDESAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240

Query: 241 INSIFTAGTPKKTGNSMTST-TSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSRE 300
           +NSIFTAGTPKKTGNS+TST  SED N+ERKSKSGQTSTCSSATSFSRSCL+KNSPCS E
Sbjct: 241 LNSIFTAGTPKKTGNSVTSTGVSEDHNSERKSKSGQTSTCSSATSFSRSCLSKNSPCS-E 300

Query: 301 KLRNSDRRTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDY 360
           KLRN+D RTVRFYPVSTIVDEY RPCGHKSL EE+ KF+VK K +T EATAA RKI+TDY
Sbjct: 301 KLRNADTRTVRFYPVSTIVDEYSRPCGHKSLCEEQPKFEVKNKAETAEATAAVRKIVTDY 360

Query: 361 QQNRKKNDFLIRNFHHPDNDLYEEE--EEEDRASCSSSDLFELDHLAVMGGDRYHEELPV 420
           QQ RKKNDFLIR FHHPDNDLYEEE  EE+D ASCSSSDLFELDHL VM GDRY EELPV
Sbjct: 361 QQIRKKNDFLIRKFHHPDNDLYEEEEQEEDDTASCSSSDLFELDHLTVMSGDRYREELPV 420

Query: 421 YETTTRVETNRAIRH 427
           YETTTRV+TNRAIRH
Sbjct: 421 YETTTRVDTNRAIRH 434

BLAST of MS008616 vs. ExPASy Swiss-Prot
Match: Q9SLL2 (Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 5.8e-42
Identity = 166/426 (38.97%), Postives = 225/426 (52.82%), Query Frame = 0

Query: 12  RFRNGGDGQ--SFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMIGKAQSDIED 71
           R RN  D +  SFSS+LLD+IYRSIDD     S   K       K Q +         ED
Sbjct: 16  RLRNRRDHRHPSFSSTLLDQIYRSIDDSSTNSSSMRK------TKHQNR---------ED 75

Query: 72  EEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVLFFSSTSSSS 131
                                 +VS  RR     ++    +      E V F  S+SSSS
Sbjct: 76  ---------------------TRVSANRRDDFNRSKNLKTI------EPVFFKHSSSSSS 135

Query: 132 DSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQRQNREKPQSK 191
           DSS  GFSSS+++  Y     +P   A   PKP+RT++     E+ E   + +R    +K
Sbjct: 136 DSS--GFSSSESDYFYRRSKSSP---AISHPKPIRTTV-----ERFERSPQNHRPNSSNK 195

Query: 192 QSNQFVINKESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFT-AGTPKKTG 251
           Q +   +         K+KS+ALKIYS+LKKVKQPISPGGRL++F+NSIFT AG  KK  
Sbjct: 196 QEHGSFL---------KTKSKALKIYSDLKKVKQPISPGGRLATFLNSIFTGAGNTKKLN 255

Query: 252 NSMTSTTSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDRRTVRFYPV 311
              T+ TS    T   + +  T+TCSSA+SFSRSCL+K +P S EK     +R+VRF PV
Sbjct: 256 KINTTVTS---TTAAAAAASSTTTCSSASSFSRSCLSK-TPSSSEK----SKRSVRFCPV 315

Query: 312 STIVDEYC--------RPCGHKSLYEEELKFQVKEKV--QTTEATAAARKILTDYQQNRK 371
           + I DE          +  G+     E ++  ++ +V  +      AA+++L  YQ   K
Sbjct: 316 NVIFDEDSSKYNNKNNKVYGNNEREYESIRHTLENRVMEENRRVIEAAKELLRSYQ---K 364

Query: 372 KNDFLIRNFHHPDNDLYEEEEEEDRASCSSSDLFELDHLAVMGGDRYHEELPVYETTTRV 425
           KN  +I      D    EE++++D  SC+SSDLFELD+L+ +G DRY EELPVYE TTR+
Sbjct: 376 KNKEVIEVSVEDD----EEDDDDDALSCTSSDLFELDNLSAIGIDRYREELPVYE-TTRL 364

BLAST of MS008616 vs. ExPASy Swiss-Prot
Match: Q9LVK2 (Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 8.4e-41
Identity = 155/413 (37.53%), Postives = 218/413 (52.78%), Query Frame = 0

Query: 21  SFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMIGKAQSDIEDEEIASLHRALL 80
           SFSS+LLD+IYRSIDD       E       + KK+     +  S  ED EI+ ++    
Sbjct: 18  SFSSTLLDQIYRSIDDSSPPPPLE------SIKKKKHHHQQRNASLHEDREISPIYHRRS 77

Query: 81  IERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSD 140
           I    E+                + RK    +H          S SSSSDSS  GFSSS+
Sbjct: 78  IAADFER----------------SRRKTDFLRH----------SNSSSSDSS--GFSSSE 137

Query: 141 TESMYG--SKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQRQNREKPQSKQSNQFVINK 200
           ++S +G    S +P   ++ +PKP+RTS    +    +P           K+   F+   
Sbjct: 138 SDSFHGRSKSSASPPSSSRQQPKPIRTSSVDHSSAVQKP-----------KELGGFL--- 197

Query: 201 ESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFT--AGTPKKTGNSMTSTTS 260
                  ++KS+ALKIYS+LKKVKQPISPGGRL++F+NS+FT  A  PKK   + T    
Sbjct: 198 -------RTKSKALKIYSDLKKVKQPISPGGRLATFLNSLFTNAATNPKKHKKTTTVAVV 257

Query: 261 EDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDRRTVRFYPVSTIVDE-- 320
           E+P++        +STCSSA+SFSRSCL+K +P S  K     +R+VRF PV+ I+DE  
Sbjct: 258 EEPHS--------SSTCSSASSFSRSCLSK-TPSSSGK----SKRSVRFCPVNVILDEDS 317

Query: 321 -YCRPCGHKS--LYEEELKFQVKEKVQTTEATAAARKILTDYQQNRKKNDFLIRNFHHPD 380
            +  P  + +  LY+     +V+E  +  +   AA+ +L  Y    K     I N     
Sbjct: 318 SFTMPYAYNNERLYDNNEAKRVEEHRRVIQ---AAKDLLRTYHNKNKVTTTNINN----- 351

Query: 381 NDLYEEEEEEDRASCSSSDLFELDHLAVMGGDRYHEELPVYETTTRVETNRAI 425
               EE++E+D ASC+SSDLFEL++L+ +G +RY EELPVYETT     NR I
Sbjct: 378 ---VEEDDEDDAASCASSDLFELENLSAIGIERYREELPVYETTRLDNMNRVI 351

BLAST of MS008616 vs. ExPASy Swiss-Prot
Match: Q9LYH0 (Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 7.9e-23
Identity = 133/415 (32.05%), Postives = 189/415 (45.54%), Query Frame = 0

Query: 21  SFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMIGKAQSDIEDEEIASLHRALL 80
           SFSS+LLDEIY SID               K  K Q                        
Sbjct: 9   SFSSTLLDEIYNSIDP--------------KTQKTQ------------------------ 68

Query: 81  IERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSD 140
                   V    +T +++S+       +    DR     FF S SSSSDS+   FSSSD
Sbjct: 69  ------PYVGSVNTTTKKQSIVTRSVPDRKIHRDR-----FFGSVSSSSDSNSSIFSSSD 128

Query: 141 TESMYGSK--SLTPSCFAKFRPKPVRTSISAPAPEKSEPKQRQNRE----KPQSKQSNQF 200
           TE  +G K  S  P CF   + KP +T        + +    QNR           S+  
Sbjct: 129 TELTHGKKTTSSRPLCFGPSKTKPRKT--------EDKTLFHQNRATRVYDDYDYASDVP 188

Query: 201 VINKESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFTAGTPKKTGNSMTS- 260
             N+   +++     R++K   N KK K P SPGGR+ +F+NS+F+  +  K  N++ S 
Sbjct: 189 KFNRHDENWENTRNRRSVKSSGNQKKPKTPASPGGRIVNFLNSLFSNNS--KQSNAVKSY 248

Query: 261 ---TTSEDPNTERKSKS---GQTSTCSSATSFSRSCLNKNSPCSREKLRNSDRRTVRFYP 320
              T+ +D    RK+ +     T+TCSSA+SFSRSC+NK      EK     +R+VRF P
Sbjct: 249 PRKTSYDDSAYVRKTSNDYHSSTTTCSSASSFSRSCMNK----GYEKSSGRIKRSVRFSP 308

Query: 321 VSTIVDEYCRPCGHKSLYEEELKFQVKEKVQ---TTEATAAARKILTDYQQNRKKNDFLI 380
           V+ IV E               +  VK+ V+          AR+ L DY +N + +    
Sbjct: 309 VNVIVPESFTSKEEDYFSNGNARKSVKKNVEDGGRRSVEEIAREFLRDYHKNHENSLVKT 356

Query: 381 RNFHHPDNDLYEEEEEEDRASCSSSDLFELDHLAVMGGDRYH----EELPVYETT 416
             F   ++D  E+++++D AS SSSDLFELD   ++G   +H    +ELPVYETT
Sbjct: 369 NGFEDYEDD-DEDDDDDDVASDSSSDLFELD---LVGNHHHHNVYGDELPVYETT 356

BLAST of MS008616 vs. ExPASy Swiss-Prot
Match: Q9M2B3 (Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.1e-20
Identity = 129/420 (30.71%), Postives = 190/420 (45.24%), Query Frame = 0

Query: 21  SFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMIGKAQSDIEDEEIASLHRALL 80
           SFSSS+LD +YRSID+  D    +LK     + +           + +D+++ +L RA++
Sbjct: 11  SFSSSVLDSVYRSIDE-SDGLQSDLK---GSINENVSSSSSSPSPNKKDDKLTTLRRAIM 70

Query: 81  IERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSD 140
                                            D E  +   SST++++ S    FSSS+
Sbjct: 71  ---------------------------------DEEHWLYARSSTTTTNSSDSSSFSSSE 130

Query: 141 TESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQRQNREKPQSKQSNQFVINKES 200
            ES    + L                      + +E  +R   E+ ++K++   V++ +S
Sbjct: 131 AESYRTKRRLR---------------------KLAEQGKRSGDERQRTKRT---VMDNDS 190

Query: 201 RDF---DEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFTAGTPKKTGNSMTSTTSE 260
           R F   D+  K +A+KI   LK+ KQP+SPG RL+SF+NSIF +   K    S+  TT  
Sbjct: 191 RLFSKSDDDKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQSNAKKVKLCSVGKTTD- 250

Query: 261 DPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDRRTVRFYPVSTIVDEYCR 320
                   KS  + +C S T       N N+ C + +      R++RFYPV   +D  CR
Sbjct: 251 -------VKSSSSKSCFSRTRNKTD--NNNNNCKKLE------RSIRFYPVRVTIDGDCR 310

Query: 321 PCGHKSLY-------EEELKFQVKEKVQT----TEATAAARKI-LTDYQQNRKKNDFLIR 380
               K +        E   K  VKE+++T    TE T   R I L D+ ++ K       
Sbjct: 311 DYAQKHITRVRKPIPEFTAKKSVKEEIKTNDHHTEFTCITRNIGLKDFVRSNK------- 335

Query: 381 NFHHPDNDLYE-EEEEEDRASCSSSDLFELDHLAVMGGDRYHEELPVYETTTRVETNRAI 425
                    YE +EEEED  S SSSDLFELD   + G  RY +ELPVYETT   +TN+AI
Sbjct: 371 ---------YEGKEEEEDAWSHSSSDLFELDSYRI-GMGRYLKELPVYETTD-FKTNQAI 335

BLAST of MS008616 vs. ExPASy Swiss-Prot
Match: B8APC7 (Protein BIG GRAIN 1 OS=Oryza sativa subsp. indica OX=39946 GN=BG1 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 5.1e-14
Identity = 91/307 (29.64%), Postives = 133/307 (43.32%), Query Frame = 0

Query: 123 SSTSSSSD-SSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQRQ 182
           ++TSSSS+ SS+GGFSSS+ ES +             R +P+RT++   AP  +  K   
Sbjct: 87  ATTSSSSECSSYGGFSSSEAESSHHR-----------RLRPIRTTVPGGAPGPAPEK--- 146

Query: 183 NREKPQSKQSNQFVINKESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFTA 242
                                   K+K     I + L+ +++P SPG RL+ F+NSIF  
Sbjct: 147 ------------------------KAKKPGASIRAKLRDLRKPASPGARLAGFLNSIFAG 206

Query: 243 GTPKKTGNSMTSTTSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDRR 302
                            P T   + +G  S CS+A+S+SRSCL+K         R   +R
Sbjct: 207 --------------KRAPATPPSATAGAESACSTASSYSRSCLSKT-----PSTRGQAKR 266

Query: 303 TVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDYQQNRKKND 362
           TVRF    T           +SL    +  + +  V+  +     R  +           
Sbjct: 267 TVRFLDSDT-----------ESLASSTVVDRRRVPVEAVQQMLLQRMEM----------- 299

Query: 363 FLIRNFHHPDNDLYEEEEEEDRASCSSSDLFELDHLAVM--GGDRYHEELPVYETTTRVE 422
                         E +E++D +S +SSDLFEL++ A +   G  Y +ELPVYE TTRV 
Sbjct: 327 --------------ESDEDDDESSDASSDLFELENFAAIAPAGAAYRDELPVYE-TTRVA 299

Query: 423 TNRAIRH 427
            NRAI H
Sbjct: 387 LNRAIGH 299

BLAST of MS008616 vs. ExPASy TrEMBL
Match: A0A6J1CUW5 (protein BIG GRAIN 1-like B OS=Momordica charantia OX=3673 GN=LOC111014541 PE=3 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 1.6e-228
Identity = 425/429 (99.07%), Postives = 426/429 (99.30%), Query Frame = 0

Query: 1   MNGQEIRARGERFRNGGDGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMI 60
           MNGQEIRARGERFRNGGDGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMI
Sbjct: 1   MNGQEIRARGERFRNGGDGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMI 60

Query: 61  GKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVL 120
           GKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVL
Sbjct: 61  GKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVL 120

Query: 121 FFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQR 180
           FFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQR
Sbjct: 121 FFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQR 180

Query: 181 QNREKPQSKQSNQFVINKESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFT 240
           QNREKPQSKQSNQFVINKESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFT
Sbjct: 181 QNREKPQSKQSNQFVINKESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFT 240

Query: 241 AGTPKKTGNSMTSTTSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDR 300
           AGTPKKTGNSMTSTTSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDR
Sbjct: 241 AGTPKKTGNSMTSTTSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDR 300

Query: 301 RTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDYQQNRKKN 360
           RTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDYQQNRKKN
Sbjct: 301 RTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDYQQNRKKN 360

Query: 361 DFLIRNFHHPDNDLYEEEEEE---DRASCSSSDLFELDHLAVMGGDRYHEELPVYETTTR 420
           DFLIRNFHHPDNDLYEEEEEE   DRASCSSSDLFELDHLAVMGGDRYHEELPVYETTTR
Sbjct: 361 DFLIRNFHHPDNDLYEEEEEEEEDDRASCSSSDLFELDHLAVMGGDRYHEELPVYETTTR 420

Query: 421 VETNRAIRH 427
           V+TNRAIRH
Sbjct: 421 VDTNRAIRH 429

BLAST of MS008616 vs. ExPASy TrEMBL
Match: A0A6J1IQB0 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111477221 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 9.2e-192
Identity = 375/436 (86.01%), Postives = 393/436 (90.14%), Query Frame = 0

Query: 1   MNGQEIRARGERFRNGGD-GQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKM 60
           M+GQE+R RGERFR+GG   QSFSSSLLDEIYRSIDDGGDKRSGELKFYR KV+KKQGKM
Sbjct: 1   MSGQEVRVRGERFRDGGGIVQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKM 60

Query: 61  IGKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDV 120
           IGKAQSD+EDEEIASLHRALLIERWIEKKVAEKVS QRRRSLTEAE KFQLYQ DREEDV
Sbjct: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEIKFQLYQQDREEDV 120

Query: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQ 180
           LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTS+SAP PEK+E KQ
Sbjct: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKTEEKQ 180

Query: 181 RQNREKPQSKQSNQFVINKE-SRDFDE----KSKSRALKIYSNLKKVKQPISPGGRLSSF 240
           RQ+REKP SKQ NQFV N E SR FDE    KSKSRALKIYSNLKKVKQPISPGGRLSSF
Sbjct: 181 RQSREKPPSKQINQFVENNEKSRGFDESAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240

Query: 241 INSIFTAGTPKKTGNSMTST-TSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSRE 300
           +NSIFTAGTPKKTGNS+TST  SED N+ERKSKSGQTSTCSSA SFSRSCL+KNSPCS E
Sbjct: 241 LNSIFTAGTPKKTGNSVTSTRASEDHNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSE 300

Query: 301 KLRNSDRRTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDY 360
           KLRN+D RTVRFYPVSTIVDEY RPCGHKSL EEE KF+VK K +  E+TAA RKI+TDY
Sbjct: 301 KLRNADTRTVRFYPVSTIVDEYSRPCGHKSLCEEEPKFEVKNKAEAAESTAAVRKIVTDY 360

Query: 361 QQNRKKNDFLIRNFHHPDNDLYEEEEEE---DRASCSSSDLFELDHLAVMGGDRYHEELP 420
           QQ RKKNDFLIR FHHPDNDLYEEEEEE   D ASCSSSDLFELDHL VM GDRY EELP
Sbjct: 361 QQIRKKNDFLIRKFHHPDNDLYEEEEEEEEDDTASCSSSDLFELDHLTVMSGDRYREELP 420

Query: 421 VYETTTRVETNRAIRH 427
           VYETTTRV+TNRAIRH
Sbjct: 421 VYETTTRVDTNRAIRH 436

BLAST of MS008616 vs. ExPASy TrEMBL
Match: A0A6J1FE05 (protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111443225 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.6e-191
Identity = 373/434 (85.94%), Postives = 395/434 (91.01%), Query Frame = 0

Query: 1   MNGQEIRARGERFRNGGD-GQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKM 60
           M+GQE+RARGERFR+GG   QSFSSSLLDEIYRSIDDGGDKRSGELKFYR KV+KKQGKM
Sbjct: 1   MSGQEVRARGERFRDGGGIVQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKM 60

Query: 61  IGKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDV 120
           IGKAQSD+EDEEIASLHRALLIERWIEKKVAEKVS QRRRSLTEAE KFQLYQ +REEDV
Sbjct: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEIKFQLYQQEREEDV 120

Query: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQ 180
           LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTS+SAP PEK+E KQ
Sbjct: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKTEEKQ 180

Query: 181 RQNREKPQSKQSNQFVINKE-SRDFDE----KSKSRALKIYSNLKKVKQPISPGGRLSSF 240
           RQ+REKP +KQ NQFV N E SR FDE    KSKSRALKIYSNLKKVKQPISPGGRLSSF
Sbjct: 181 RQSREKPPAKQINQFVENNEKSRGFDESAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240

Query: 241 INSIFTAGTPKKTGNSMTST-TSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSRE 300
           +NSIFTAGTPKKTGNS+TST  SED N+ERKSKSGQTSTCSSA SFSRSCL+KNSPCS E
Sbjct: 241 LNSIFTAGTPKKTGNSVTSTGVSEDHNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSE 300

Query: 301 KLRNSDRRTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDY 360
           KLRN+D RTVRFYPVSTIVDEY RPCGHKSL EE+ KF+VK K +T EATAA RKI+TDY
Sbjct: 301 KLRNADTRTVRFYPVSTIVDEYSRPCGHKSLCEEQPKFEVKNKAETAEATAAVRKIVTDY 360

Query: 361 QQNRKKNDFLIRNFHHPDNDLYEEE-EEEDRASCSSSDLFELDHLAVMGGDRYHEELPVY 420
           QQ RKKNDFLIR FHHPDNDLYEEE EE+D ASCSSSDLFELDHL VM GDRY EELPVY
Sbjct: 361 QQIRKKNDFLIRKFHHPDNDLYEEEQEEDDTASCSSSDLFELDHLTVMSGDRYREELPVY 420

Query: 421 ETTTRVETNRAIRH 427
           ETTTR++TNRAIRH
Sbjct: 421 ETTTRMDTNRAIRH 434

BLAST of MS008616 vs. ExPASy TrEMBL
Match: A0A0A0L438 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G646410 PE=3 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 1.2e-186
Identity = 371/440 (84.32%), Postives = 399/440 (90.68%), Query Frame = 0

Query: 1   MNGQEIRAR-GERFRNG--GDGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQG 60
           M+G+E+R R GERFRNG  G+GQSFSSSLLDEIYRSIDDGGDKRSGELKFYR KV+KKQG
Sbjct: 1   MSGEEVRVRSGERFRNGGCGNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQG 60

Query: 61  KMIGKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREE 120
           K+IGKAQSD+EDEEIASLHRALLIERWIEKKVAEKVS QRRRSLTE E KFQLYQHDREE
Sbjct: 61  KVIGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEGEIKFQLYQHDREE 120

Query: 121 DVLFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEP 180
           DVLFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKP+RTS+SAP PEK+E 
Sbjct: 121 DVLFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPIRTSVSAPPPEKTEA 180

Query: 181 KQRQNREKPQSKQSNQFVINKE-SRDFDE----KSKSRALKIYSNLKKVKQPISPGGRLS 240
           KQRQ+REKPQSKQSNQFV NKE SR FDE    KSKSRALKIYSNLKKVKQPISPGGRLS
Sbjct: 181 KQRQSREKPQSKQSNQFVENKEKSRGFDENAMIKSKSRALKIYSNLKKVKQPISPGGRLS 240

Query: 241 SFINSIFTAGTPKKTGNSMTSTT-SEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCS 300
           SF+NSIFTAGTPKKTGNS++STT SEDPN+ERKSKSGQTSTCSSATSFSRSCL+K+SPCS
Sbjct: 241 SFLNSIFTAGTPKKTGNSVSSTTLSEDPNSERKSKSGQTSTCSSATSFSRSCLSKSSPCS 300

Query: 301 REKLRNSDRRTVRFYPVSTIVDEYCRPCGHKSLYEEE-LKFQVKEKVQTTEATAAARKIL 360
            EKLRN+DRRTVRFYPVSTIVDEYCRPCGHKSLYEEE  KFQ+K K ++ E+T  +RK  
Sbjct: 301 SEKLRNADRRTVRFYPVSTIVDEYCRPCGHKSLYEEEDPKFQIKNKAESAEST--SRKNT 360

Query: 361 TDYQQN-RKKNDFLIRNFHHPDNDLYEEEEEEDRASCSSSDLFELDHLAVMGG---DRYH 420
           TDYQQ  RKKNDFLIRNFHHPDNDL EE++E   ASCSSSDLFELDHL  M G   +RY 
Sbjct: 361 TDYQQQMRKKNDFLIRNFHHPDNDLSEEDDE--TASCSSSDLFELDHLREMSGGCSNRYR 420

Query: 421 EELPVYETTTRVETNRAIRH 427
           EELPVYE+TTRV+TNRAIRH
Sbjct: 421 EELPVYESTTRVDTNRAIRH 436

BLAST of MS008616 vs. ExPASy TrEMBL
Match: A0A6J1FHL7 (protein BIG GRAIN 1-like A isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445856 PE=3 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.6e-186
Identity = 366/433 (84.53%), Postives = 385/433 (88.91%), Query Frame = 0

Query: 1   MNGQEIRARGERFRN-GGDGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKM 60
           M+GQE+RARGER R+ GG GQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKV+KKQGK 
Sbjct: 84  MSGQEVRARGERLRDGGGKGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVLKKQGKT 143

Query: 61  IGKAQSDIEDEEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDV 120
           IG       DEEIASLHRALLIERWIEKKVAEKVS QRRRSLTEAE KFQLYQHDREEDV
Sbjct: 144 IG-------DEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEMKFQLYQHDREEDV 203

Query: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQ 180
           LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTS+SA  PEK+E KQ
Sbjct: 204 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSALPPEKTEAKQ 263

Query: 181 RQNREKPQSKQSNQFVINKESRDFDE----KSKSRALKIYSNLKKVKQPISPGGRLSSFI 240
           RQ+REKPQSKQSNQFV NKESRD DE    KSKSRALKIY NLKKVKQPISPGGRLSSF+
Sbjct: 264 RQSREKPQSKQSNQFVENKESRDLDENAMIKSKSRALKIYDNLKKVKQPISPGGRLSSFL 323

Query: 241 NSIFTAGTPKKTGNSMTST-TSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREK 300
           NSIFTAGTPKK  +S+TST  SEDPN+ERKSKSGQTSTCSSATSFSRSCL+KNSPCS EK
Sbjct: 324 NSIFTAGTPKKQAHSVTSTGASEDPNSERKSKSGQTSTCSSATSFSRSCLSKNSPCSSEK 383

Query: 301 LRNSDRRTVRFYPVSTIVDEYCRPCGHKSLYEEELKFQVKEKVQTTEATAAARKILTDYQ 360
           L N DRRTVRFYPVSTIVDE+C+PCGHKSLY+E  KFQVK+K QT EATAAAR ILTDYQ
Sbjct: 384 LGNVDRRTVRFYPVSTIVDEHCKPCGHKSLYDEGPKFQVKDKPQTAEATAAARNILTDYQ 443

Query: 361 QNRKKNDFLIRNFHHPDNDLY-EEEEEEDRASCSSSDLFELDHLAVMGGDRYHEELPVYE 420
           Q R K DFL R FHHP+N LY EEEEE+D ASCSSSDLFELDHL VM GDRY EELPVYE
Sbjct: 444 QIRNKKDFLARKFHHPNNHLYQEEEEEDDTASCSSSDLFELDHLTVMSGDRYREELPVYE 503

Query: 421 TTTRVETNRAIRH 427
            TT V+TNRAIRH
Sbjct: 504 RTTGVDTNRAIRH 509

BLAST of MS008616 vs. TAIR 10
Match: AT1G54200.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1084 Blast hits to 581 proteins in 136 species: Archae - 0; Bacteria - 72; Metazoa - 212; Fungi - 78; Plants - 102; Viruses - 0; Other Eukaryotes - 620 (source: NCBI BLink). )

HSP 1 Score: 173.3 bits (438), Expect = 4.1e-43
Identity = 166/426 (38.97%), Postives = 225/426 (52.82%), Query Frame = 0

Query: 12  RFRNGGDGQ--SFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMIGKAQSDIED 71
           R RN  D +  SFSS+LLD+IYRSIDD     S   K       K Q +         ED
Sbjct: 16  RLRNRRDHRHPSFSSTLLDQIYRSIDDSSTNSSSMRK------TKHQNR---------ED 75

Query: 72  EEIASLHRALLIERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVLFFSSTSSSS 131
                                 +VS  RR     ++    +      E V F  S+SSSS
Sbjct: 76  ---------------------TRVSANRRDDFNRSKNLKTI------EPVFFKHSSSSSS 135

Query: 132 DSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQRQNREKPQSK 191
           DSS  GFSSS+++  Y     +P   A   PKP+RT++     E+ E   + +R    +K
Sbjct: 136 DSS--GFSSSESDYFYRRSKSSP---AISHPKPIRTTV-----ERFERSPQNHRPNSSNK 195

Query: 192 QSNQFVINKESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFT-AGTPKKTG 251
           Q +   +         K+KS+ALKIYS+LKKVKQPISPGGRL++F+NSIFT AG  KK  
Sbjct: 196 QEHGSFL---------KTKSKALKIYSDLKKVKQPISPGGRLATFLNSIFTGAGNTKKLN 255

Query: 252 NSMTSTTSEDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDRRTVRFYPV 311
              T+ TS    T   + +  T+TCSSA+SFSRSCL+K +P S EK     +R+VRF PV
Sbjct: 256 KINTTVTS---TTAAAAAASSTTTCSSASSFSRSCLSK-TPSSSEK----SKRSVRFCPV 315

Query: 312 STIVDEYC--------RPCGHKSLYEEELKFQVKEKV--QTTEATAAARKILTDYQQNRK 371
           + I DE          +  G+     E ++  ++ +V  +      AA+++L  YQ   K
Sbjct: 316 NVIFDEDSSKYNNKNNKVYGNNEREYESIRHTLENRVMEENRRVIEAAKELLRSYQ---K 364

Query: 372 KNDFLIRNFHHPDNDLYEEEEEEDRASCSSSDLFELDHLAVMGGDRYHEELPVYETTTRV 425
           KN  +I      D    EE++++D  SC+SSDLFELD+L+ +G DRY EELPVYE TTR+
Sbjct: 376 KNKEVIEVSVEDD----EEDDDDDALSCTSSDLFELDNLSAIGIDRYREELPVYE-TTRL 364

BLAST of MS008616 vs. TAIR 10
Match: AT3G13980.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 1485 Blast hits to 418 proteins in 98 species: Archae - 0; Bacteria - 6; Metazoa - 246; Fungi - 61; Plants - 107; Viruses - 6; Other Eukaryotes - 1059 (source: NCBI BLink). )

HSP 1 Score: 169.5 bits (428), Expect = 6.0e-42
Identity = 155/413 (37.53%), Postives = 218/413 (52.78%), Query Frame = 0

Query: 21  SFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMIGKAQSDIEDEEIASLHRALL 80
           SFSS+LLD+IYRSIDD       E       + KK+     +  S  ED EI+ ++    
Sbjct: 18  SFSSTLLDQIYRSIDDSSPPPPLE------SIKKKKHHHQQRNASLHEDREISPIYHRRS 77

Query: 81  IERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSD 140
           I    E+                + RK    +H          S SSSSDSS  GFSSS+
Sbjct: 78  IAADFER----------------SRRKTDFLRH----------SNSSSSDSS--GFSSSE 137

Query: 141 TESMYG--SKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQRQNREKPQSKQSNQFVINK 200
           ++S +G    S +P   ++ +PKP+RTS    +    +P           K+   F+   
Sbjct: 138 SDSFHGRSKSSASPPSSSRQQPKPIRTSSVDHSSAVQKP-----------KELGGFL--- 197

Query: 201 ESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFT--AGTPKKTGNSMTSTTS 260
                  ++KS+ALKIYS+LKKVKQPISPGGRL++F+NS+FT  A  PKK   + T    
Sbjct: 198 -------RTKSKALKIYSDLKKVKQPISPGGRLATFLNSLFTNAATNPKKHKKTTTVAVV 257

Query: 261 EDPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDRRTVRFYPVSTIVDE-- 320
           E+P++        +STCSSA+SFSRSCL+K +P S  K     +R+VRF PV+ I+DE  
Sbjct: 258 EEPHS--------SSTCSSASSFSRSCLSK-TPSSSGK----SKRSVRFCPVNVILDEDS 317

Query: 321 -YCRPCGHKS--LYEEELKFQVKEKVQTTEATAAARKILTDYQQNRKKNDFLIRNFHHPD 380
            +  P  + +  LY+     +V+E  +  +   AA+ +L  Y    K     I N     
Sbjct: 318 SFTMPYAYNNERLYDNNEAKRVEEHRRVIQ---AAKDLLRTYHNKNKVTTTNINN----- 351

Query: 381 NDLYEEEEEEDRASCSSSDLFELDHLAVMGGDRYHEELPVYETTTRVETNRAI 425
               EE++E+D ASC+SSDLFEL++L+ +G +RY EELPVYETT     NR I
Sbjct: 378 ---VEEDDEDDAASCASSDLFELENLSAIGIERYREELPVYETTRLDNMNRVI 351

BLAST of MS008616 vs. TAIR 10
Match: AT5G12050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 109.8 bits (273), Expect = 5.6e-24
Identity = 133/415 (32.05%), Postives = 189/415 (45.54%), Query Frame = 0

Query: 21  SFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMIGKAQSDIEDEEIASLHRALL 80
           SFSS+LLDEIY SID               K  K Q                        
Sbjct: 9   SFSSTLLDEIYNSIDP--------------KTQKTQ------------------------ 68

Query: 81  IERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSD 140
                   V    +T +++S+       +    DR     FF S SSSSDS+   FSSSD
Sbjct: 69  ------PYVGSVNTTTKKQSIVTRSVPDRKIHRDR-----FFGSVSSSSDSNSSIFSSSD 128

Query: 141 TESMYGSK--SLTPSCFAKFRPKPVRTSISAPAPEKSEPKQRQNRE----KPQSKQSNQF 200
           TE  +G K  S  P CF   + KP +T        + +    QNR           S+  
Sbjct: 129 TELTHGKKTTSSRPLCFGPSKTKPRKT--------EDKTLFHQNRATRVYDDYDYASDVP 188

Query: 201 VINKESRDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFTAGTPKKTGNSMTS- 260
             N+   +++     R++K   N KK K P SPGGR+ +F+NS+F+  +  K  N++ S 
Sbjct: 189 KFNRHDENWENTRNRRSVKSSGNQKKPKTPASPGGRIVNFLNSLFSNNS--KQSNAVKSY 248

Query: 261 ---TTSEDPNTERKSKS---GQTSTCSSATSFSRSCLNKNSPCSREKLRNSDRRTVRFYP 320
              T+ +D    RK+ +     T+TCSSA+SFSRSC+NK      EK     +R+VRF P
Sbjct: 249 PRKTSYDDSAYVRKTSNDYHSSTTTCSSASSFSRSCMNK----GYEKSSGRIKRSVRFSP 308

Query: 321 VSTIVDEYCRPCGHKSLYEEELKFQVKEKVQ---TTEATAAARKILTDYQQNRKKNDFLI 380
           V+ IV E               +  VK+ V+          AR+ L DY +N + +    
Sbjct: 309 VNVIVPESFTSKEEDYFSNGNARKSVKKNVEDGGRRSVEEIAREFLRDYHKNHENSLVKT 356

Query: 381 RNFHHPDNDLYEEEEEEDRASCSSSDLFELDHLAVMGGDRYH----EELPVYETT 416
             F   ++D  E+++++D AS SSSDLFELD   ++G   +H    +ELPVYETT
Sbjct: 369 NGFEDYEDD-DEDDDDDDVASDSSSDLFELD---LVGNHHHHNVYGDELPVYETT 356

BLAST of MS008616 vs. TAIR 10
Match: AT3G42800.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 853 Blast hits to 340 proteins in 98 species: Archae - 0; Bacteria - 94; Metazoa - 145; Fungi - 62; Plants - 95; Viruses - 0; Other Eukaryotes - 457 (source: NCBI BLink). )

HSP 1 Score: 101.7 bits (252), Expect = 1.5e-21
Identity = 129/420 (30.71%), Postives = 190/420 (45.24%), Query Frame = 0

Query: 21  SFSSSLLDEIYRSIDDGGDKRSGELKFYRHKVMKKQGKMIGKAQSDIEDEEIASLHRALL 80
           SFSSS+LD +YRSID+  D    +LK     + +           + +D+++ +L RA++
Sbjct: 11  SFSSSVLDSVYRSIDE-SDGLQSDLK---GSINENVSSSSSSPSPNKKDDKLTTLRRAIM 70

Query: 81  IERWIEKKVAEKVSTQRRRSLTEAERKFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSD 140
                                            D E  +   SST++++ S    FSSS+
Sbjct: 71  ---------------------------------DEEHWLYARSSTTTTNSSDSSSFSSSE 130

Query: 141 TESMYGSKSLTPSCFAKFRPKPVRTSISAPAPEKSEPKQRQNREKPQSKQSNQFVINKES 200
            ES    + L                      + +E  +R   E+ ++K++   V++ +S
Sbjct: 131 AESYRTKRRLR---------------------KLAEQGKRSGDERQRTKRT---VMDNDS 190

Query: 201 RDF---DEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIFTAGTPKKTGNSMTSTTSE 260
           R F   D+  K +A+KI   LK+ KQP+SPG RL+SF+NSIF +   K    S+  TT  
Sbjct: 191 RLFSKSDDDKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQSNAKKVKLCSVGKTTD- 250

Query: 261 DPNTERKSKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNSDRRTVRFYPVSTIVDEYCR 320
                   KS  + +C S T       N N+ C + +      R++RFYPV   +D  CR
Sbjct: 251 -------VKSSSSKSCFSRTRNKTD--NNNNNCKKLE------RSIRFYPVRVTIDGDCR 310

Query: 321 PCGHKSLY-------EEELKFQVKEKVQT----TEATAAARKI-LTDYQQNRKKNDFLIR 380
               K +        E   K  VKE+++T    TE T   R I L D+ ++ K       
Sbjct: 311 DYAQKHITRVRKPIPEFTAKKSVKEEIKTNDHHTEFTCITRNIGLKDFVRSNK------- 335

Query: 381 NFHHPDNDLYE-EEEEEDRASCSSSDLFELDHLAVMGGDRYHEELPVYETTTRVETNRAI 425
                    YE +EEEED  S SSSDLFELD   + G  RY +ELPVYETT   +TN+AI
Sbjct: 371 ---------YEGKEEEEDAWSHSSSDLFELDSYRI-GMGRYLKELPVYETTD-FKTNQAI 335

BLAST of MS008616 vs. TAIR 10
Match: AT1G69160.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 173 Blast hits to 172 proteins in 54 species: Archae - 0; Bacteria - 0; Metazoa - 25; Fungi - 33; Plants - 84; Viruses - 2; Other Eukaryotes - 29 (source: NCBI BLink). )

HSP 1 Score: 49.7 bits (117), Expect = 6.9e-06
Identity = 70/253 (27.67%), Postives = 111/253 (43.87%), Query Frame = 0

Query: 201 RDFDEKSKSRALKIYSNLKKVKQPISPGGRLSSFINSIF-TAGTPKKTGNSMTSTTSEDP 260
           +D  EK +   +K      + KQP SPGG+++SF+NS+F  AG+ K    S + T   DP
Sbjct: 93  QDHHEKHEVTTIKERLGNVRHKQPSSPGGKIASFLNSLFHQAGSKKNKSKSKSKTKPTDP 152

Query: 261 NTERK---------------------SKSGQTSTCSSATSFSRSCLNKNSPCSREKLRNS 320
             E +                     S+S  T+T ++A+S S+S ++ +S         S
Sbjct: 153 EVEEEIPGGGWMRRRRRSSISHFFSSSRSTSTTTTTTASSSSKSLISSSS---------S 212

Query: 321 DRRTVRFYPVSTIVDEYCRPCGH----KSLYEEELKFQ-----VKEKVQTTEATAAARKI 380
             RT   Y ++T    Y +   +    K + EEE K       + EK++  E+ +  ++I
Sbjct: 213 GFRTPPPY-LNTPTKNYKQFLNYTSATKQVGEEETKTNKEYSWLDEKLKVMESLSENQRI 272

Query: 381 LTDYQQNRKKNDFLIRNFHHPDNDLYEEEEEEDRASCSSSDLFELDHLAVMGGDRYHEEL 423
            +D +                D  +  E E++   S SSSDLFEL +  +  G      L
Sbjct: 273 WSDDED------------IDDDRRIKREGEDDGMESDSSSDLFELQNYELSRGG-----L 318