MS008448 (gene) Bitter gourd (TR) v1

Overview


Name	MS008448
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ribosomal RNA large subunit methyltransferase H
Location	scaffold4: 1489943 .. 1491470 (+)
RNA-Seq Expression	MS008448
Synteny	MS008448

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAATGGACAGAGAGCGGTGCCAATACGGGTTTTGACTGTGGGGAAGAAGAGGTCTCGTGGCGTGCAACTCGTCGTTGATGAGTATATTGAAAAGCTTAAGCATTACTGTAACGTAGAAGATGTTCAACTGCGGTCAAATCCTAGGAATGCGCGGTATGCGACTGTACTGTTCGTGTTAATTAGTAAATTTTCTTATCACCTTCTTATCTCTGACGAATGATGCTAGTCTTATCAAAACTTCTTCGTTCTATGTATTGATGTTTGAATTTTAGGGATCCCAGGGCTCAGGTTGACGATGAAGATATGGCTGTACTGAATCTTCTCAAGTCTGACGATTGGGTTTGTCTCTCAACATTCATTTTTGTCGGATCACTATATATTTTGTTGCTCTGGCTGTGCTCGTTGGTATAAACTAGTTTAGAACTCTCTATCTCAATTACATCATTTGTCAGCCAAAAATGTGAAACAAAATTGATTTCTTGAACTTTTTACTTTAATATAGATAAGTCACATGGCCCCCAAGTCACAGCTCAGATGGCAAGGGTTTGAAGTCTCTACTAGCTTAGAGGCCTTAAGTTCGAATCTTACAGGGAGCTTAATACCAAAAATCTTTCGAGTCCTGGTCCTAGAGCGGGGGTCATATATATATATGTATGTATGTATGTATGTATGTATGTATATGTATATGTATGTATGTAGATGGGTCACAGTAGTGCTTCTTTTCCAACAGAAAAACATAGGTTGCAATGTGCTTCTCTTTCTAACCCTCAGATAAAAACTGATCTGTTGAATGGTTTATAGTAGCGGCGCTTACAGCTTAGTCTCCATTCTGCATATGCACCTGCAGGTTGTTATGTTGGATGAGAATGGGAAAGAGATGAGCTCTGAGCGGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGTGTGTACTATGTCCAACTCAAATCAAATTGGCTGCTTCATTATCATCAGTTCATCACTACTGGACAGTATTCATGATAGTTAATTTTTCAAAGTTATTAGCTAAGGAGTTTGGACTGGAAATATAAAGCTATGCTTATGGTACCTCCCAGTTTAGTTAGCCCACATGTTCATCTTCCATTTCAGTTTAGATTTCACCACGTGATAGTCTCTTGCTTTCTAATTTTATGTAATGGACATGCACAGATGTTTGTCTACTATATATGGTTTTTTTAAGGGTTTTTTTAAAGCTTAATTATGCTTTCTGATTAACACCATGCTATAATGGTTTCAGAAAACAATTTTTAGGAGAGTCAAGGGGTGTGCCAAGATTGGACATGGCATTTTGTAGATGAATCTTGATTAGGTACTGTCTCTTGATTTCTTCTTTTCTTCAGGGTGCTTCAAGATTATCATTTTGCATTGGGGGACCTTATGGCCATGGGCAACAACTGCGACAACGAGCTAACTTAACAATCAAGCTGTCCTCCATGGTCTTAAATCATCAAATTGCATTACTTGTACTTATGGAACAACTTTACAGGTTACTTTTTTTTACC

mRNA sequence

ATGGACAATGGACAGAGAGCGGTGCCAATACGGGTTTTGACTGTGGGGAAGAAGAGGTCTCGTGGCGTGCAACTCGTCGTTGATGAGTATATTGAAAAGCTTAAGCATTACTGTAACGTAGAAGATGTTCAACTGCGGTCAAATCCTAGGAATGCGCGGGATCCCAGGGCTCAGGTTGACGATGAAGATATGGCTGTACTGAATCTTCTCAAGTCTGACGATTGGGTTGTTATGTTGGATGAGAATGGGAAAGAGATGAGCTCTGAGCGGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGGTGCTTCAAGATTATCATTTTGCATTGGGGGACCTTATGGCCATGGGCAACAACTGCGACAACGAGCTAACTTAACAATCAAGCTGTCCTCCATGGTCTTAAATCATCAAATTGCATTACTTGTACTTATGGAACAACTTTACAGGTTACTTTTTTTTACC

Coding sequence (CDS)

ATGGACAATGGACAGAGAGCGGTGCCAATACGGGTTTTGACTGTGGGGAAGAAGAGGTCTCGTGGCGTGCAACTCGTCGTTGATGAGTATATTGAAAAGCTTAAGCATTACTGTAACGTAGAAGATGTTCAACTGCGGTCAAATCCTAGGAATGCGCGGGATCCCAGGGCTCAGGTTGACGATGAAGATATGGCTGTACTGAATCTTCTCAAGTCTGACGATTGGGTTGTTATGTTGGATGAGAATGGGAAAGAGATGAGCTCTGAGCGGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGGTGCTTCAAGATTATCATTTTGCATTGGGGGACCTTATGGCCATGGGCAACAACTGCGACAACGAGCTAACTTAACAATCAAGCTGTCCTCCATGGTCTTAAATCATCAAATTGCATTACTTGTACTTATGGAACAACTTTACAGGTTACTTTTTTTTACC

Protein sequence

MDNGQRAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMAVLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRANLTIKLSSMVLNHQIALLVLMEQLYRLLFFT

Homology

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BLAST of MS008448 vs. NCBI nr
Match: XP_022131264.1 (putative RNA methyltransferase At5g10620 isoform X2 [Momordica charantia])

HSP 1 Score: 280.8 bits (717), Expect = 7.1e-72
Identity = 144/145 (99.31%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA
Sbjct: 30  RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 89

Query: 66  VLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 125
           V+NLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN
Sbjct: 90  VVNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 149

Query: 126 LTIKLSSMVLNHQIALLVLMEQLYR 151
           LTIKLSSMVLNHQIALLVLMEQLYR
Sbjct: 150 LTIKLSSMVLNHQIALLVLMEQLYR 174

[Close]

BLAST of MS008448 vs. NCBI nr
Match: XP_022159857.1 (putative RNA methyltransferase At5g10620 isoform X1 [Momordica charantia])

HSP 1 Score: 280.8 bits (717), Expect = 7.1e-72
Identity = 144/145 (99.31%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA
Sbjct: 32  RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 91

Query: 66  VLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 125
           V+NLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN
Sbjct: 92  VVNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 151

Query: 126 LTIKLSSMVLNHQIALLVLMEQLYR 151
           LTIKLSSMVLNHQIALLVLMEQLYR
Sbjct: 152 LTIKLSSMVLNHQIALLVLMEQLYR 176

[Close]

BLAST of MS008448 vs. NCBI nr
Match: XP_038896820.1 (putative RNA methyltransferase At5g10620 isoform X1 [Benincasa hispida])

HSP 1 Score: 277.3 bits (708), Expect = 7.9e-71
Identity = 141/152 (92.76%), Postives = 150/152 (98.68%), Query Frame = 0


Query: 1   MDNGQRAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVD 60
           MDNGQRAVPIRVLTVGKKRSRGVQL+VDEYIEKLK+YC++EDV LRSNPRNARDPRAQVD
Sbjct: 24  MDNGQRAVPIRVLTVGKKRSRGVQLLVDEYIEKLKNYCHIEDVLLRSNPRNARDPRAQVD 83

Query: 61  DEDMAVLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQL 120
           DEDMAV+NLLKSDDWVVMLDENGKEMSSE+MA+LVADAG TGASRLSFCIGGPYGHGQQL
Sbjct: 84  DEDMAVVNLLKSDDWVVMLDENGKEMSSEQMADLVADAGTTGASRLSFCIGGPYGHGQQL 143

Query: 121 RQRANLTIKLSSMVLNHQIALLVLMEQLYRLL 153
           RQRANL+IKLSSMVLNHQIALLVLMEQLYR+L
Sbjct: 144 RQRANLSIKLSSMVLNHQIALLVLMEQLYRVL 175

[Close]

BLAST of MS008448 vs. NCBI nr
Match: XP_038896822.1 (putative RNA methyltransferase At5g10620 isoform X3 [Benincasa hispida])

HSP 1 Score: 276.6 bits (706), Expect = 1.3e-70
Identity = 140/150 (93.33%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MDNGQRAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVD 60
           MDNGQRAVPIRVLTVGKKRSRGVQL+VDEYIEKLK+YC++EDV LRSNPRNARDPRAQVD
Sbjct: 24  MDNGQRAVPIRVLTVGKKRSRGVQLLVDEYIEKLKNYCHIEDVLLRSNPRNARDPRAQVD 83

Query: 61  DEDMAVLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQL 120
           DEDMAV+NLLKSDDWVVMLDENGKEMSSE+MA+LVADAG TGASRLSFCIGGPYGHGQQL
Sbjct: 84  DEDMAVVNLLKSDDWVVMLDENGKEMSSEQMADLVADAGTTGASRLSFCIGGPYGHGQQL 143

Query: 121 RQRANLTIKLSSMVLNHQIALLVLMEQLYR 151
           RQRANL+IKLSSMVLNHQIALLVLMEQLYR
Sbjct: 144 RQRANLSIKLSSMVLNHQIALLVLMEQLYR 173

[Close]

BLAST of MS008448 vs. NCBI nr
Match: KAG7037551.1 (putative RNA methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 273.1 bits (697), Expect = 1.5e-69
Identity = 137/150 (91.33%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MDNGQRAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVD 60
           MDN QRAVPIRVLTVGKKRSRGVQL++DEYI+KL +YCNVEDVQLRSNPRNARDPRAQVD
Sbjct: 1   MDNEQRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVD 60

Query: 61  DEDMAVLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQL 120
           DED+AV+NLLKSDDWVVMLDENGKE+SSE+MAELVADAGNTGASRL+FCIGGPYGHGQQL
Sbjct: 61  DEDIAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQL 120

Query: 121 RQRANLTIKLSSMVLNHQIALLVLMEQLYR 151
           RQRAN++IKLSSMVLNHQIALLVLMEQLYR
Sbjct: 121 RQRANVSIKLSSMVLNHQIALLVLMEQLYR 150

[Close]

BLAST of MS008448 vs. ExPASy Swiss-Prot
Match: Q9LXB4 (Putative RNA methyltransferase At5g10620 OS=Arabidopsis thaliana OX=3702 GN=At5g10620 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 9.1e-54
Identity = 104/145 (71.72%), Postives = 130/145 (89.66%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RA+PIRV+TVGKKRS GV+L+VDEY  KLK YC+ ED  +RSNPRNA+D RAQV+DE++A
Sbjct: 37  RALPIRVITVGKKRSEGVRLLVDEYKIKLKPYCSFEDSLVRSNPRNAQDVRAQVEDEEVA 96

Query: 66  VLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 125
           ++ L+ SDDWVV+LDE G+++ SE+MAEL+ DAGN+GASR+SFCIGG YGHG Q+R+RAN
Sbjct: 97  MMKLIGSDDWVVVLDERGRDIDSEQMAELLGDAGNSGASRISFCIGGAYGHGTQVRKRAN 156

Query: 126 LTIKLSSMVLNHQIALLVLMEQLYR 151
           +TI+LSSMVLNHQIAL+VLMEQLYR
Sbjct: 157 VTIRLSSMVLNHQIALVVLMEQLYR 181

[Close]

BLAST of MS008448 vs. ExPASy Swiss-Prot
Match: A0M0D0 (Ribosomal RNA large subunit methyltransferase H OS=Gramella forsetii (strain KT0803) OX=411154 GN=rlmH PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.1e-18
Identity = 53/146 (36.30%), Postives = 84/146 (57.53%), Query Frame = 0


Query: 10  IRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVE-----DVQLRSNPRNARDPRAQVDDEDM 69
           I+++ +GK   + ++ ++  Y ++L+HY   E     D++   N     D   Q   E  
Sbjct: 3   IKLVCIGKTDKKELEALIKIYSDRLQHYIKFEFEIIPDLKKTKN----LDENQQKSKEGE 62

Query: 70  AVLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRA 129
            +L+ +++ D+VV+LDENGK+ SSE  +E +    NTG  RL F IGGPYG  +++ +RA
Sbjct: 63  LILSGVQNSDFVVLLDENGKQFSSESFSEYIQKRMNTGLKRLIFVIGGPYGFSEEVYKRA 122

Query: 130 NLTIKLSSMVLNHQIALLVLMEQLYR 151
           +  I LS M  +HQ+  L   EQLYR
Sbjct: 123 DSKISLSKMTFSHQMVRLFFTEQLYR 144

[Close]

BLAST of MS008448 vs. ExPASy Swiss-Prot
Match: A0PXE8 (Ribosomal RNA large subunit methyltransferase H OS=Clostridium novyi (strain NT) OX=386415 GN=rlmH PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 2.3e-17
Identity = 52/145 (35.86%), Postives = 82/145 (56.55%), Query Frame = 0


Query: 10  IRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQL--RSNPRNA--RDPRAQVDDEDMA 69
           I ++ VGK + + ++L +DEY ++L  YC +  V+L     P NA  +D       E   
Sbjct: 3   ITIICVGKLKEKYLKLAIDEYSKRLSRYCKLNIVELNDEKTPDNASEKDELIIKQKEGSK 62

Query: 70  VLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 129
           +L  +K + +V+ LD NGK  SSE +AEL+ D G  G S ++  IGG  G  +++  RAN
Sbjct: 63  ILAHVKENMYVIALDLNGKMASSEELAELIGDLGLKGKSNIALVIGGSLGLSKEVLNRAN 122

Query: 130 LTIKLSSMVLNHQIALLVLMEQLYR 151
             +  S M   HQ+  ++L+EQ+YR
Sbjct: 123 YKLSFSKMTFPHQLMRVILLEQIYR 147

[Close]

BLAST of MS008448 vs. ExPASy Swiss-Prot
Match: Q1DCX5 (Ribosomal RNA large subunit methyltransferase H OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=rlmH PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 4.0e-17
Identity = 56/141 (39.72%), Postives = 78/141 (55.32%), Query Frame = 0


Query: 10  IRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMAVLNL 69
           +R+L++GK RS   +  V EY ++L HY   E V++         P      E  A+L+ 
Sbjct: 3   VRLLSIGKDRSGLYEPAVQEYAKRLGHYTRFELVEMAEASGKRLKPGDAKAAEAEAILSK 62

Query: 70  LKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRANLTIK 129
            K  DW+V LDE G  + S  ++  V  A  TGA  L F IGG  G   ++R  ANLT+ 
Sbjct: 63  RKPQDWLVALDERGSLLDSVELSRYVGKA-QTGAKDLLFIIGGDEGLDARVRDAANLTLS 122

Query: 130 LSSMVLNHQIALLVLMEQLYR 151
           LS M L H++A +VL+EQLYR
Sbjct: 123 LSKMTLPHRLARVVLIEQLYR 142

[Close]

BLAST of MS008448 vs. ExPASy Swiss-Prot
Match: B2TRC7 (Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain Eklund 17B / Type B) OX=935198 GN=rlmH PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.4e-16
Identity = 50/145 (34.48%), Postives = 85/145 (58.62%), Query Frame = 0


Query: 10  IRVLTVGKKRSRGVQLVVDEYIEKLKHYC--NVEDVQLRSNPRNA--RDPRAQVDDEDMA 69
           I +++VGK + + ++  +DEY ++L  YC  N+ ++Q    P NA  +D     D E   
Sbjct: 3   ITIISVGKLKEKYLKHAIDEYSKRLSRYCKLNILELQDEQTPDNASEKDELIIKDKEGNK 62

Query: 70  VLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 129
           +LN +K + +V+ LD  GK MSSE +++ + + G  G S L F IGG  G  + + +R+N
Sbjct: 63  ILNSIKDNMYVITLDLKGKMMSSEELSKFIDNCGVRGNSNLCFVIGGSLGLSEAVLKRSN 122

Query: 130 LTIKLSSMVLNHQIALLVLMEQLYR 151
            ++  S M   HQ+  ++L+EQ+YR
Sbjct: 123 HSLCFSKMTFPHQLFRVMLLEQIYR 147

[Close]

BLAST of MS008448 vs. ExPASy TrEMBL
Match: A0A6J1E0Z2 (putative RNA methyltransferase At5g10620 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111026123 PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 3.5e-72
Identity = 144/145 (99.31%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA
Sbjct: 32  RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 91

Query: 66  VLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 125
           V+NLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN
Sbjct: 92  VVNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 151

Query: 126 LTIKLSSMVLNHQIALLVLMEQLYR 151
           LTIKLSSMVLNHQIALLVLMEQLYR
Sbjct: 152 LTIKLSSMVLNHQIALLVLMEQLYR 176

[Close]

BLAST of MS008448 vs. ExPASy TrEMBL
Match: A0A6J1BPR4 (putative RNA methyltransferase At5g10620 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111026123 PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 3.5e-72
Identity = 144/145 (99.31%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA
Sbjct: 30  RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 89

Query: 66  VLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 125
           V+NLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN
Sbjct: 90  VVNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 149

Query: 126 LTIKLSSMVLNHQIALLVLMEQLYR 151
           LTIKLSSMVLNHQIALLVLMEQLYR
Sbjct: 150 LTIKLSSMVLNHQIALLVLMEQLYR 174

[Close]

BLAST of MS008448 vs. ExPASy TrEMBL
Match: A0A6J1BP95 (putative RNA methyltransferase At5g10620 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111026123 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.4e-68
Identity = 141/145 (97.24%), Postives = 142/145 (97.93%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNA   RAQVDDEDMA
Sbjct: 32  RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNA---RAQVDDEDMA 91

Query: 66  VLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 125
           V+NLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN
Sbjct: 92  VVNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 151

Query: 126 LTIKLSSMVLNHQIALLVLMEQLYR 151
           LTIKLSSMVLNHQIALLVLMEQLYR
Sbjct: 152 LTIKLSSMVLNHQIALLVLMEQLYR 173

[Close]

BLAST of MS008448 vs. ExPASy TrEMBL
Match: A0A6J1FPF2 (putative RNA methyltransferase At5g10620 OS=Cucurbita moschata OX=3662 GN=LOC111446064 PE=3 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 2.0e-67
Identity = 133/145 (91.72%), Postives = 144/145 (99.31%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQL++DEYI+KL +YCNVEDVQLRSNPRNARDPRAQVDDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 85

Query: 66  VLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 125
           V+NLLKSDDWVVMLDENGKE+SSE+MAELVADAGNTGASRL+FCIGGPYGHGQQLRQRAN
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 145

Query: 126 LTIKLSSMVLNHQIALLVLMEQLYR 151
           ++IKLSSMVLNHQIALLVLMEQLYR
Sbjct: 146 VSIKLSSMVLNHQIALLVLMEQLYR 170

[Close]

BLAST of MS008448 vs. ExPASy TrEMBL
Match: A0A1S3CQZ8 (putative RNA methyltransferase At5g10620 OS=Cucumis melo OX=3656 GN=LOC103503770 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 5.7e-67
Identity = 132/145 (91.03%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQL+VDEYI+KLK+YC+VEDVQLRSNPRNARDP+AQ+DDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLVDEYIDKLKNYCHVEDVQLRSNPRNARDPKAQIDDEDVA 85

Query: 66  VLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 125
           V+NLLKSDDWVVMLDENGKE+SSE+MAELVADAGNTGASRLSFCIGGPYGHGQ+LR+RAN
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLSFCIGGPYGHGQKLRERAN 145

Query: 126 LTIKLSSMVLNHQIALLVLMEQLYR 151
           L+IKLSSMVLNHQIALLVLMEQLYR
Sbjct: 146 LSIKLSSMVLNHQIALLVLMEQLYR 170

[Close]

BLAST of MS008448 vs. TAIR 10
Match: AT5G10620.1 (methyltransferases )

HSP 1 Score: 211.1 bits (536), Expect = 6.5e-55
Identity = 104/145 (71.72%), Postives = 130/145 (89.66%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RA+PIRV+TVGKKRS GV+L+VDEY  KLK YC+ ED  +RSNPRNA+D RAQV+DE++A
Sbjct: 37  RALPIRVITVGKKRSEGVRLLVDEYKIKLKPYCSFEDSLVRSNPRNAQDVRAQVEDEEVA 96

Query: 66  VLNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 125
           ++ L+ SDDWVV+LDE G+++ SE+MAEL+ DAGN+GASR+SFCIGG YGHG Q+R+RAN
Sbjct: 97  MMKLIGSDDWVVVLDERGRDIDSEQMAELLGDAGNSGASRISFCIGGAYGHGTQVRKRAN 156

Query: 126 LTIKLSSMVLNHQIALLVLMEQLYR 151
           +TI+LSSMVLNHQIAL+VLMEQLYR
Sbjct: 157 VTIRLSSMVLNHQIALVVLMEQLYR 181

[Close]

BLAST of MS008448 vs. TAIR 10
Match: AT5G10605.1 (methyltransferases )

HSP 1 Score: 55.1 bits (131), Expect = 5.9e-08
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 0


Query: 111 GGPYGHGQQLRQRANLTIKLSSMVLNHQIALLVLME 147
           G  YGHG+QLR+RAN+ I+LSSMVLNH IAL+VLME
Sbjct: 21  GVAYGHGRQLRKRANVAIRLSSMVLNHHIALVVLME 56

[Close]

BLAST of MS008448 vs. TAIR 10
Match: AT5G10605.2 (methyltransferases )

HSP 1 Score: 55.1 bits (131), Expect = 5.9e-08
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 0


Query: 111 GGPYGHGQQLRQRANLTIKLSSMVLNHQIALLVLME 147
           G  YGHG+QLR+RAN+ I+LSSMVLNH IAL+VLME
Sbjct: 21  GVAYGHGRQLRKRANVAIRLSSMVLNHHIALVVLME 56

The following BLAST results are available for this feature:

BLAST of MS008448 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131264.1	7.1e-72	99.31	putative RNA methyltransferase At5g10620 isoform X2 [Momordica charantia]	[more]
XP_022159857.1	7.1e-72	99.31	putative RNA methyltransferase At5g10620 isoform X1 [Momordica charantia]	[more]
XP_038896820.1	7.9e-71	92.76	putative RNA methyltransferase At5g10620 isoform X1 [Benincasa hispida]	[more]
XP_038896822.1	1.3e-70	93.33	putative RNA methyltransferase At5g10620 isoform X3 [Benincasa hispida]	[more]
KAG7037551.1	1.5e-69	91.33	putative RNA methyltransferase [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MS008448 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LXB4	9.1e-54	71.72	Putative RNA methyltransferase At5g10620 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
A0M0D0	8.1e-18	36.30	Ribosomal RNA large subunit methyltransferase H OS=Gramella forsetii (strain KT0...	[more]
A0PXE8	2.3e-17	35.86	Ribosomal RNA large subunit methyltransferase H OS=Clostridium novyi (strain NT)...	[more]
Q1DCX5	4.0e-17	39.72	Ribosomal RNA large subunit methyltransferase H OS=Myxococcus xanthus (strain DK...	[more]
B2TRC7	3.4e-16	34.48	Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain...	[more]

BLAST of MS008448 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E0Z2	3.5e-72	99.31	putative RNA methyltransferase At5g10620 isoform X1 OS=Momordica charantia OX=36...	[more]
A0A6J1BPR4	3.5e-72	99.31	putative RNA methyltransferase At5g10620 isoform X2 OS=Momordica charantia OX=36...	[more]
A0A6J1BP95	1.4e-68	97.24	putative RNA methyltransferase At5g10620 isoform X3 OS=Momordica charantia OX=36...	[more]
A0A6J1FPF2	2.0e-67	91.72	putative RNA methyltransferase At5g10620 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A1S3CQZ8	5.7e-67	91.03	putative RNA methyltransferase At5g10620 OS=Cucumis melo OX=3656 GN=LOC103503770...	[more]

BLAST of MS008448 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10620.1	6.5e-55	71.72	methyltransferases	[more]
AT5G10605.1	5.9e-08	75.00	methyltransferases	[more]
AT5G10605.2	5.9e-08	75.00	methyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 7..153 e-value: 2.9E-42 score: 145.8
IPR003742	RNA methyltransferase RlmH	PIRSF	PIRSF004505	MT_bac	coord: 8..154 e-value: 2.0E-46 score: 155.9
IPR003742	RNA methyltransferase RlmH	PFAM	PF02590	SPOUT_MTase	coord: 10..151 e-value: 2.2E-40 score: 138.2
IPR003742	RNA methyltransferase RlmH	PANTHER	PTHR33603	METHYLTRANSFERASE	coord: 5..151
IPR003742	RNA methyltransferase RlmH	CDD	cd18081	RlmH-like	coord: 10..150 e-value: 9.12279E-55 score: 167.619
None	No IPR available	SFLD	SFLDS00070	SPOUT_Methyltransferase	coord: 4..152 e-value: 0.0 score: 233.6
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 10..151

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS008448.1	MS008448.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006364	rRNA processing
molecular_function	GO:0008168	methyltransferase activity